RchiOBHm_Chr6g0285231

Sterol 3-beta-glucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001484
Physical Location & Seq
Forward (+)
48572896 .. 48580957
8062 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25584

Sequence Viewer

Length: 2133 bp
ATGAAGCACACCATCATCACTCATACCCCTCCATCGCCACCCACTTTTATCGAACGTGCCCGTCTCTTCCGGACAGAATCCAATTCTCTCTTTCTTTTTCTTCTCCGATTCTCTCTCTCCCTCTCCCTCTCCGATTCTCAATTTCGAATCTCACAGCGTTCTCAGAATCCAAGCCACGCTCTTCTTCTTCTTCTCGCTTCCGATCTGGATCAGGACCGGCCGCGCGGTCCGGAGTGTCATCCGGAACAGGCGGCGGTGATGGCGGACGATCGCCGGAGTAGTTCCGAGAGTTCTGTAGAAGTTGCGGGGAGAATTGAGCAGGAAATTGACGGCGGAAGTGGCGGGAAAAGATTAGAGTTGGAGAACGGAGAGGCTAGTGGCGGAGGCGGAGGCGTTACTGCTCCGGTGAATAACGGCGGCGGCAGTAGCTCTACAGGGAATTCAGGAAGTTCTGGCTTTAGCTTTTCGAAAACGAACGCATTGCCAGTGGAGATATCACCGACTCATGAAAGGCCAGAACCACATTCAGTTCCAGCAAAGCTGGAGAGATCAAAAACGGAGAGCACCAGACCTAATGTATTGTCCAAAGAGGCAGCAAAGATTTTTGACGACAAAACTTCAGTCCATCAGAAGTTAAAGCTGTTAAATAGAATAGCCACTGTGAAAGATGACGGAACTGTAGAATTCGATGTTCCAGGGGATGTTGAATCTGAATCTTTTGGTGTTGGATCCACTGCTGGTTGTACCGATGTTGCTGATGAGCCTCTTGATGCAACAGAGCTCCAATGCATACCACCAATGCAAATTGTAATGCTTATTGTTGGAACGCGTGGAGATGTGCAGCCGTTTATTGCAATTGGCAAGCGTTTGCAGGACTATGGCCATCGTGTAAGACTAGCTACTCATTCAAATTTTAAAGAGTTTGTCTTGACTGCTGGTTTGGAGTTCTACCCTTTAGGCGGTGATCCAAAAGTTCTTGCTGGCTATATGGTTAAAAATAAAGGCTTCTTGCCATCAGGGCCTTCGGAGATTCCCATTCAGCGTAACCAAATAAAGGAAATTATATATTCCTTACTTCCAGCTTGCAAAGAGCCTGATGTAGATTCTGGTATCCCCTTTAAAGCGGATGCAATTATTGCAAACCCCCCAGCATATGGGCATACTCATGTGGCGGAGGCACTGAAAATACCTCTTCATATATTTTTTACAATGCCATGGACGCCAACAAGTGAATTTCCACATCCCTTGTCCCGCGTTAAACAACAAACTGGATATAGACTATCGTATCAAATTGTTGACTCCTTAATATGGCTTGGAATACGGGACATGATTAATGATGTTCGGAAGAAAAAATTGAGATTACGACCTGTCACATATTTAAGCGGTTCACAAGGCTCTGAGTCTGATGTCCCACATGGATACATATGGAGTCCTCATCTTGTTCCGAAACCAAAAGATTGGGGACCTAAGGTTGATGTGGTGGGATTTTGCTTCCTTGATCTTGCATCAAATTATGAACCTCCACAACCACTAGTGGAATGGCTTAAAGCTGGTGACAAGCCTATATACATTGGGTTTGGTAGCCTTCCTGTTCAAGAGCCAGAGAAAATGACCCAAATAATCGTCGATGCATTAGAAAAGACAGGACAGAGAGGCATCATTAACAAAGGCTGGGGTGGCCTTGGCAACTTGGCTGAACCAAAGGAATCCATATACTTATTGGACAACTGCCCTCATGACTGGCTGTTTCTACAATGCAAGGCAGTGGTGCATCATGGAGGTGCTGGAACAACGGCTGCTGGTCTAAAAGCTGCATGTCCGACAACAATTGTTCCTTTCTTTGGAGACCAACCCTTCTGGGGAGAGCGAGTACATGCCAGGGGAGTAGGCCCTGCACCCATTGCAGTTGATGAGTTCTCACTTCCCAAATTGGTTGATGCTATAAATTACATGCTTGATCCCAAGGTGAAGGAGCGTGCTATTGAACTTGCCAAGGATATGGAGAATGAGGATGGTGTGACAGGAGCAGTCATGGCCTTCTTTAAGCATCTTCCTCGCAAAAAGCCCGAGTCTGAGCCAGAAACAGAGTCATCGAGCCTTTGCTCTATAAGTAGATGTTTTGGTTGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

710

Amino Acids

77.45

Weight (kDa)

6.02

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 269 - 412 6.5e-35 Glycosyltransferase family 28 N-terminal domain
UDPGT PF00201 518 - 664 6e-06 UDP-glucoronosyl and UDP-glucosyl transferase
EryCIII-like_C PF06722 570 - 669 2.4e-11 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1154
AccII CGCG 4 cut(s) 223, 225, 829, 1254
AccIII TCCGGA 3 cut(s) 69, 229, 241
AclWI GGATC 5 cut(s) 216, 723, 736, 959, 1952
AcoI YGGCCR 2 cut(s) 218, 880
AcsI RAATTY 4 cut(s) 439, 683, 910, 1232
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 1 cut(s) 1220
AfaI GTAC 2 cut(s) 745, 1872
AfiI CCNNNNNNNGG 6 cut(s) 959, 1054, 1154, 1308, 1841, 1859
AflIII ACRYGT 1 cut(s) 827
AgsI TTSAA 4 cut(s) 707, 909, 1595, 1985
AhlI ACTAGT 1 cut(s) 1531
AjnI CCWGG 2 cut(s) 694, 1877
AluBI AGCT 9 cut(s) 429, 462, 541, 640, 781, 899, 1082, 1550, 1811
AluI AGCT 9 cut(s) 429, 462, 541, 640, 781, 899, 1082, 1550, 1811
Alw21I GWGCWC 2 cut(s) 566, 783
Alw26I GTCTC 2 cut(s) 68, 1839
AlwI GGATC 5 cut(s) 216, 723, 736, 959, 1952
Ama87I CYCGRG 1 cut(s) 2066
Aor13HI TCCGGA 3 cut(s) 69, 229, 241
AoxI GGCC 7 cut(s) 218, 512, 880, 1019, 1678, 1888, 2034
ApeKI GCWGC 4 cut(s) 593, 841, 1796, 1811
ApoI RAATTY 4 cut(s) 439, 683, 910, 1232
ArsI GACNNNNNNTTYG 4 cut(s) 606, 638, 922, 954
AseI ATTAAT 1 cut(s) 1332
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 5 cut(s) 214, 227, 1019, 1463, 1889
AsuHPI GGTGA 6 cut(s) 268, 418, 489, 974, 1565, 1978
AsuII TTCGAA 2 cut(s) 145, 467
AvaI CYCGRG 1 cut(s) 2066
AvaII GGWCC 3 cut(s) 214, 227, 1463
AxyI CCTNAGG 1 cut(s) 1467
BaeGI GKGCMC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 1268, 1301
BalI TGGCCA 1 cut(s) 882
BamHI GGATCC 1 cut(s) 728
BanII GRGCYC 1 cut(s) 783
Bbv12I GWGCWC 2 cut(s) 566, 783
BbvI GCAGC 4 cut(s) 605, 853, 1783, 1798
BccI CCATC 7 cut(s) 20, 40, 253, 633, 891, 1021, 2006
BceAI ACGGC 4 cut(s) 346, 430, 829, 1809
BcgI CGANNNNNNTGC 4 cut(s) 463, 497, 2036, 2070
BciT130I CCWGG 2 cut(s) 696, 1879
BciVI GTATCC 2 cut(s) 1121, 1412
BcoDI GTCTC 2 cut(s) 68, 1839
BcuI ACTAGT 1 cut(s) 1531
BfaI CTAG 3 cut(s) 375, 896, 1532
BfmI CTRYAG 3 cut(s) 294, 432, 678
BfuI GTATCC 2 cut(s) 1121, 1412
BglI GCCNNNNNGGC 1 cut(s) 1018
BisI GCNGC 8 cut(s) 221, 252, 418, 421, 594, 842, 1797, 1812
BlsI GCNGC 8 cut(s) 222, 253, 419, 422, 595, 843, 1798, 1813
Bme1390I CCNGG 2 cut(s) 696, 1879
Bme18I GGWCC 3 cut(s) 214, 227, 1463
BmeT110I CYCGRG 1 cut(s) 2066
BmgT120I GGNCC 5 cut(s) 214, 227, 1019, 1463, 1889
BmiI GGNNCC 2 cut(s) 730, 1464
BmrFI CCNGG 2 cut(s) 696, 1879
BmsI GCATC 8 cut(s) 760, 1117, 1514, 1618, 1665, 1780, 1927, 2056
BpmI CTGGAG 1 cut(s) 563
Bpu14I TTCGAA 2 cut(s) 145, 467
BsaBI GATNNNNATC 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 1220
BsaI GGTCTC 1 cut(s) 1839
BsaJI CCNNGG 6 cut(s) 695, 1214, 1681, 1878, 1962, 1992
BsaWI WCCGGW 4 cut(s) 69, 229, 241, 403
BsaXI ACNNNNNCTCC 2 cut(s) 1854, 1884
Bsc4I CCNNNNNNNGG 6 cut(s) 959, 1054, 1154, 1308, 1841, 1859
Bse118I RCCGGY 1 cut(s) 216
Bse1I ACTGG 3 cut(s) 485, 1273, 1745
Bse21I CCTNAGG 1 cut(s) 1467
Bse3DI GCAATG 2 cut(s) 479, 1899
Bse8I GATNNNNATC 1 cut(s) 207
BseAI TCCGGA 3 cut(s) 69, 229, 241
BseBI CCWGG 2 cut(s) 696, 1879
BseDI CCNNGG 6 cut(s) 695, 1214, 1681, 1878, 1962, 1992
BseGI GGATG 5 cut(s) 238, 706, 1132, 1240, 2017
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 6 cut(s) 959, 1054, 1154, 1308, 1841, 1859
BseMI GCAATG 2 cut(s) 479, 1899
BseMII CTCAG 3 cut(s) 176, 1389, 2064
BseNI ACTGG 3 cut(s) 485, 1273, 1745
BseSI GKGCMC 1 cut(s) 61
BseX3I CGGCCG 1 cut(s) 218
BseXI GCAGC 4 cut(s) 605, 853, 1783, 1798
BseYI CCCAGC 2 cut(s) 1147, 1671
BsgI GTGCAG 2 cut(s) 860, 1878
Bsh1236I CGCG 4 cut(s) 223, 225, 829, 1254
Bsh1285I CGRYCG 2 cut(s) 221, 271
BshFI GGCC 7 cut(s) 220, 514, 882, 1021, 1680, 1890, 2036
BsiEI CGRYCG 2 cut(s) 221, 271
BsiHKAI GWGCWC 2 cut(s) 566, 783
BsiHKCI CYCGRG 1 cut(s) 2066
BsiSI CCGG 6 cut(s) 70, 217, 230, 242, 274, 404
BslFI GGGAC 4 cut(s) 1234, 1337, 1394, 1476
BslI CCNNNNNNNGG 6 cut(s) 959, 1054, 1154, 1308, 1841, 1859
BsmAI GTCTC 2 cut(s) 68, 1839
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 4 cut(s) 1234, 1337, 1394, 1476
BsnI GGCC 7 cut(s) 220, 514, 882, 1021, 1680, 1890, 2036
Bso31I GGTCTC 1 cut(s) 1839
BsoBI CYCGRG 1 cut(s) 2066
Bsp119I TTCGAA 2 cut(s) 145, 467
Bsp1286I GDGCHC 3 cut(s) 61, 566, 783
Bsp13I TCCGGA 3 cut(s) 69, 229, 241
Bsp143I GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
Bsp19I CCATGG 1 cut(s) 1214
BspANI GGCC 7 cut(s) 220, 514, 882, 1021, 1680, 1890, 2036
BspCNI CTCAG 3 cut(s) 175, 1390, 2065
BspEI TCCGGA 3 cut(s) 69, 229, 241
BspFNI CGCG 4 cut(s) 223, 225, 829, 1254
BspHI TCATGA 2 cut(s) 505, 1735
BspLI GGNNCC 2 cut(s) 730, 1464
BspPI GGATC 5 cut(s) 216, 723, 736, 959, 1952
BspQI GCTCTTC 1 cut(s) 186
BspT104I TTCGAA 2 cut(s) 145, 467
BspTNI GGTCTC 1 cut(s) 1839
BsrDI GCAATG 2 cut(s) 479, 1899
BsrFI RCCGGY 1 cut(s) 216
BsrI ACTGG 3 cut(s) 485, 1273, 1745
BssAI RCCGGY 1 cut(s) 216
BssECI CCNNGG 6 cut(s) 695, 1214, 1681, 1878, 1962, 1992
BssMI GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
BssNI GRCGYC 1 cut(s) 1220
BssT1I CCWWGG 4 cut(s) 1214, 1681, 1962, 1992
Bst2UI CCWGG 2 cut(s) 696, 1879
Bst4CI ACNGT 2 cut(s) 661, 679
Bst6I CTCTTC 3 cut(s) 71, 186, 1197
BstACI GRCGYC 1 cut(s) 1220
BstAPI GCANNNNNTGC 2 cut(s) 1136, 1901
BstBI TTCGAA 2 cut(s) 145, 467
BstC8I GCNNGC 4 cut(s) 863, 982, 1084, 1977
BstDEI CTNAG 4 cut(s) 162, 1398, 1467, 2073
BstDSI CCRYGG 1 cut(s) 1214
BstF5I GGATG 5 cut(s) 238, 706, 1132, 1240, 2017
BstFNI CGCG 4 cut(s) 223, 225, 829, 1254
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 8 cut(s) 205, 211, 271, 551, 731, 967, 1501, 1960
BstMAI GTCTC 2 cut(s) 68, 1839
BstMBI GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
BstMCI CGRYCG 2 cut(s) 221, 271
BstMWI GCNNNNNNNGC 9 cut(s) 260, 339, 426, 1018, 1136, 1219, 1677, 1901, 2033
BstNI CCWGG 2 cut(s) 696, 1879
BstNSI RCATGY 3 cut(s) 1818, 1877, 1954
BstSCI CCNGG 2 cut(s) 694, 1877
BstSFI CTRYAG 3 cut(s) 294, 432, 678
BstSLI GKGCMC 1 cut(s) 61
BstUI CGCG 4 cut(s) 223, 225, 829, 1254
BstV1I GCAGC 4 cut(s) 605, 853, 1783, 1798
BstX2I RGATCY 1 cut(s) 728
BstXI CCANNNNNNTGG 2 cut(s) 1458, 1999
BstYI RGATCY 1 cut(s) 728
BstZI CGGCCG 1 cut(s) 218
Bsu36I CCTNAGG 1 cut(s) 1467
BsuI GTATCC 2 cut(s) 1121, 1412
BsuRI GGCC 7 cut(s) 220, 514, 882, 1021, 1680, 1890, 2036
BtgI CCRYGG 1 cut(s) 1214
BtgZI GCGATG 1 cut(s) 18
BtsCI GGATG 5 cut(s) 238, 706, 1132, 1240, 2017
BtsI GCAGTG 2 cut(s) 732, 1770
BtsIMutI CAGTG 5 cut(s) 492, 657, 732, 1178, 1770
Cac8I GCNNGC 4 cut(s) 863, 982, 1084, 1977
CciI TCATGA 2 cut(s) 505, 1735
CfoI GCGC 1 cut(s) 225
Cfr10I RCCGGY 1 cut(s) 216
Cfr13I GGNCC 5 cut(s) 214, 227, 1019, 1463, 1889
CpoI CGGWCCG 1 cut(s) 227
CseI GACGC 1 cut(s) 1228
Csp6I GTAC 2 cut(s) 744, 1871
CspCI CAANNNNNGTGG 2 cut(s) 1227, 1262
CspI CGGWCCG 1 cut(s) 227
CviQI GTAC 2 cut(s) 744, 1871
DdeI CTNAG 4 cut(s) 162, 1398, 1467, 2073
DpnI GATC 8 cut(s) 204, 210, 270, 550, 730, 966, 1500, 1959
DpnII GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
DraI TTTAAA 2 cut(s) 916, 1120
EaeI YGGCCR 2 cut(s) 218, 880
EagI CGGCCG 1 cut(s) 218
Eam1104I CTCTTC 3 cut(s) 71, 186, 1197
EarI CTCTTC 3 cut(s) 71, 186, 1197
EciI GGCGGA 5 cut(s) 278, 348, 396, 402, 1187
Ecl136II GAGCTC 1 cut(s) 781
EclXI CGGCCG 1 cut(s) 218
Eco130I CCWWGG 4 cut(s) 1214, 1681, 1962, 1992
Eco24I GRGCYC 1 cut(s) 783
Eco31I GGTCTC 1 cut(s) 1839
Eco32I GATATC 1 cut(s) 495
Eco47I GGWCC 3 cut(s) 214, 227, 1463
Eco52I CGGCCG 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 603
Eco81I CCTNAGG 1 cut(s) 1467
Eco88I CYCGRG 1 cut(s) 2066
EcoICRI GAGCTC 1 cut(s) 781
EcoO109I RGGNCCY 3 cut(s) 1019, 1463, 1889
EcoRI GAATTC 2 cut(s) 439, 683
EcoRII CCWGG 2 cut(s) 694, 1877
EcoRV GATATC 1 cut(s) 495
EcoT14I CCWWGG 4 cut(s) 1214, 1681, 1962, 1992
EcoT22I ATGCAT 2 cut(s) 791, 1633
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 4 cut(s) 1214, 1681, 1962, 1992
Esp3I CGTCTC 1 cut(s) 68
FaqI GGGAC 4 cut(s) 1234, 1337, 1394, 1476
FauI CCCGC 3 cut(s) 298, 335, 1259
FauNDI CATATG 2 cut(s) 1153, 1424
Fnu4HI GCNGC 8 cut(s) 221, 252, 418, 421, 594, 842, 1797, 1812
FokI GGATG 5 cut(s) 225, 713, 1139, 1227, 2024
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 8 cut(s) 221, 252, 418, 421, 594, 842, 1797, 1812
FspBI CTAG 3 cut(s) 375, 896, 1532
GlaI GCGC 1 cut(s) 224
GluI GCNGC 8 cut(s) 221, 252, 418, 421, 594, 842, 1797, 1812
GsaI CCCAGC 2 cut(s) 1151, 1675
GsuI CTGGAG 1 cut(s) 563
HaeIII GGCC 7 cut(s) 220, 514, 882, 1021, 1680, 1890, 2036
HapII CCGG 6 cut(s) 70, 217, 230, 242, 274, 404
HgaI GACGC 1 cut(s) 1228
HhaI GCGC 1 cut(s) 225
Hin1I GRCGYC 1 cut(s) 1220
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 1 cut(s) 1297
HindII GTYRAC 1 cut(s) 1297
HpaII CCGG 6 cut(s) 70, 217, 230, 242, 274, 404
HphI GGTGA 6 cut(s) 268, 418, 489, 974, 1565, 1978
Hpy166II GTNNAC 2 cut(s) 1297, 1388
Hpy8I GTNNAC 2 cut(s) 1297, 1388
Hpy99I CGWCG 1 cut(s) 1628
HpyAV CCTTC 5 cut(s) 1032, 1595, 1864, 1963, 2047
HpyCH4III ACNGT 2 cut(s) 661, 679
HpyCH4IV ACGT 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 9 cut(s) 260, 339, 426, 1018, 1136, 1219, 1677, 1901, 2033
HpyF3I CTNAG 4 cut(s) 162, 1398, 1467, 2073
HpySE526I ACGT 1 cut(s) 55
Hsp92I GRCGYC 1 cut(s) 1220
HspAI GCGC 1 cut(s) 223
Kpn2I TCCGGA 3 cut(s) 69, 229, 241
Kzo9I GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
LguI GCTCTTC 1 cut(s) 186
LmnI GCTCC 4 cut(s) 406, 786, 1972, 2024
Lsp1109I GCAGC 4 cut(s) 605, 853, 1783, 1798
LweI GCATC 8 cut(s) 760, 1117, 1514, 1618, 1665, 1780, 1927, 2056
MaeI CTAG 3 cut(s) 375, 896, 1532
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 5 cut(s) 394, 1043, 1369, 1553, 2017
MalI GATC 8 cut(s) 204, 210, 270, 550, 730, 966, 1500, 1959
MboI GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
MboII GAAGA 9 cut(s) 58, 92, 173, 176, 179, 182, 1184, 1357, 2042
MfeI CAATTG 2 cut(s) 855, 1827
MflI RGATCY 1 cut(s) 728
MhlI GDGCHC 3 cut(s) 61, 566, 783
MlsI TGGCCA 1 cut(s) 882
MluI ACGCGT 1 cut(s) 827
MluNI TGGCCA 1 cut(s) 882
MlyI GAGTC 6 cut(s) 496, 1292, 1409, 1438, 2078, 2096
MmeI TCCRAC 4 cut(s) 339, 706, 802, 1844
Mox20I TGGCCA 1 cut(s) 882
Mph1103I ATGCAT 2 cut(s) 791, 1633
MroI TCCGGA 3 cut(s) 69, 229, 241
MscI TGGCCA 1 cut(s) 882
MslI CAYNNNNRTG 2 cut(s) 1164, 1779
Msp20I TGGCCA 1 cut(s) 882
MspI CCGG 6 cut(s) 70, 217, 230, 242, 274, 404
MspR9I CCNGG 2 cut(s) 696, 1879
MunI CAATTG 2 cut(s) 855, 1827
MvaI CCWGG 2 cut(s) 696, 1879
MvnI CGCG 4 cut(s) 223, 225, 829, 1254
MwoI GCNNNNNNNGC 9 cut(s) 260, 339, 426, 1018, 1136, 1219, 1677, 1901, 2033
NcoI CCATGG 1 cut(s) 1214
NdeI CATATG 2 cut(s) 1153, 1424
NdeII GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
NlaIV GGNNCC 2 cut(s) 730, 1464
NmuCI GTSAC 3 cut(s) 1369, 1553, 2017
NsiI ATGCAT 2 cut(s) 791, 1633
NspI RCATGY 3 cut(s) 1818, 1877, 1954
NspV TTCGAA 2 cut(s) 145, 467
PagI TCATGA 2 cut(s) 505, 1735
PciSI GCTCTTC 1 cut(s) 186
PflMI CCANNNNNTGG 1 cut(s) 1154
PkrI GCNGC 8 cut(s) 222, 253, 419, 422, 595, 843, 1798, 1813
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 6 cut(s) 496, 1292, 1408, 1437, 2077, 2095
PpsI GAGTC 6 cut(s) 496, 1292, 1408, 1437, 2077, 2095
PpuMI RGGWCCY 1 cut(s) 1463
PshBI ATTAAT 1 cut(s) 1332
Psp124BI GAGCTC 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 1463
Psp6I CCWGG 2 cut(s) 694, 1877
PspFI CCCAGC 2 cut(s) 1147, 1671
PspGI CCWGG 2 cut(s) 694, 1877
PspN4I GGNNCC 2 cut(s) 730, 1464
PspPI GGNCC 5 cut(s) 214, 227, 1019, 1463, 1889
PspPPI RGGWCCY 1 cut(s) 1463
PsuI RGATCY 1 cut(s) 728
PvuI CGATCG 1 cut(s) 271
RsaI GTAC 2 cut(s) 745, 1872
RsaNI GTAC 2 cut(s) 744, 1871
RseI CAYNNNNRTG 2 cut(s) 1164, 1779
Rsr2I CGGWCCG 1 cut(s) 227
RsrII CGGWCCG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 783
SapI GCTCTTC 1 cut(s) 186
SatI GCNGC 8 cut(s) 221, 252, 418, 421, 594, 842, 1797, 1812
Sau3AI GATC 8 cut(s) 202, 208, 268, 548, 728, 964, 1498, 1957
Sau96I GGNCC 5 cut(s) 214, 227, 1019, 1463, 1889
SchI GAGTC 6 cut(s) 496, 1292, 1409, 1438, 2078, 2096
ScrFI CCNGG 2 cut(s) 696, 1879
SduI GDGCHC 3 cut(s) 61, 566, 783
SfaNI GCATC 8 cut(s) 760, 1117, 1514, 1618, 1665, 1780, 1927, 2056
SfcI CTRYAG 3 cut(s) 294, 432, 678
SfuI TTCGAA 2 cut(s) 145, 467
SinI GGWCC 3 cut(s) 214, 227, 1463
SmiMI CAYNNNNRTG 2 cut(s) 1164, 1779
SpeI ACTAGT 1 cut(s) 1531
SspMI CTAG 3 cut(s) 375, 896, 1532
SstI GAGCTC 1 cut(s) 783
StyD4I CCNGG 2 cut(s) 694, 1877
StyI CCWWGG 4 cut(s) 1214, 1681, 1962, 1992
TaaI ACNGT 2 cut(s) 661, 679
TaiI ACGT 1 cut(s) 58
TaqI TCGA 6 cut(s) 51, 145, 467, 687, 1626, 2093
TatI WGTACW 1 cut(s) 1870
TauI GCSGC 4 cut(s) 223, 254, 420, 423
TscAI CASTG 5 cut(s) 492, 664, 739, 1185, 1770
TseFI GTSAC 3 cut(s) 1369, 1553, 2017
TseI GCWGC 4 cut(s) 593, 841, 1796, 1811
Tsp45I GTSAC 3 cut(s) 1369, 1553, 2017
TspDTI ATGAA 4 cut(s) 17, 522, 1184, 1530
TspGWI ACGGA 3 cut(s) 381, 572, 687
TspRI CASTG 5 cut(s) 492, 664, 739, 1185, 1770
Van91I CCANNNNNTGG 1 cut(s) 1154
VpaK11BI GGWCC 3 cut(s) 214, 227, 1463
VspI ATTAAT 1 cut(s) 1332
XapI RAATTY 4 cut(s) 439, 683, 910, 1232
XceI RCATGY 3 cut(s) 1818, 1877, 1954
XcmI CCANNNNNNNNNTGG 2 cut(s) 1536, 1717
XspI CTAG 3 cut(s) 375, 896, 1532
Zsp2I ATGCAT 2 cut(s) 791, 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.