Rroxscaffold_6G00426610

Sterol 3-beta-glucosyltransferase UGT80A2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001484
Physical Location & Seq
Reverse (-)
47284096 .. 47291486
7391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00426610.1

Sequence Viewer

Length: 1329 bp
ATGCTGATCGTTGGTACGCGAGGAGATGTTCAGCCATTTGTTGCTATTGGAAAGAAATTGCAGGAATATGGCCATAGGGTTAGGCTAGCAACTCATGCAAATTTCAAAGATTTTGTCCTTACTGCTGGGTTGGAGTTCTTTCCTTTAGGTGGAGATCCGAAAGTTCTTGCCGAATATATGGTCAAGAATAAAGGTTTCTTGCCATCGGGACCTTCGGAAGTATCTATTCAGCGACAGCAAATAAAAGAAATAATATTTTCTTTACTTCCTGCGTGTAAGGAACCTGATCCTGATTCCAAAGTCCCGTTTAAAGCAGATGCAATAATTGCCAACCCACCAGCATATGGGCACTCTCATGTTGCTGAGGCACTAAAAATACCGATTCATATATTCTTCACAATGCCATGGACGCCTACCAGTGAATTCCCTCATCCTCTATCCCGTGTCAAGCAACATATTGGATATAGACTATCATATCAAATAGTCGATGCATTAATTTGGCTAGGAATTCGAGACATGATAAATGAGTTTAGGAAGAAAATGCTGAAGCTGAGACGTACCACATATTTAAGTGGATACTATAGTACTCCTCCTGACGTTCCCTATGGGTATATATGGAGTCCTCATCTTGTTCCCAAACCTAAAGATTGGGGTCCTAAGATTGATGTCGTTGGGTTTTGTTTTCTTGACCTTGCTTCAAATTATGAACCTCCTGATTCACTTGTGAAGTGGCTTGGAGCTGGTGAACAGCCTGTCTATGTTGGATTTGGTAGCCTTCCTCTTCAAGAACCAGAGAAGATGACCAATATTATTCTTCAAGCTCTGGAAATTACTGGACAGAGAGGCATTATCAACAAAGGCTGGGGTGGCCTTGGTAATTCGGTAGAACCAAATGATTCTGTGTACTTAGTGGACAATTGCCCCCATGATTGGCTCTTCCTACGATGCTCTGCCGTGGTACATCATGGAGGTGCTGGGACAACTGCTGCTGGTTTGAAAGCTGCGTGTCCAACTACGATTGTGCCATTCTTTGGAGATCAGCCATTTTGGGGTGAGAGGGTGCATGCCAGAGGAGTAGGTCCTGCACCAATCCCAGTTGATGAGTTCTCACTTGAAAAATTGGTTGATGCCATACGGTTCATGTTAGATCCAAAGGTTAAAGAGCGTGCTGTTGAAATTGCCAAAGCAATGGAGAATGAAGATGGGGTCGCCGGAGCAGTGAAGGCCTTTCATAAGCACTTTCCTCGCAATAAAGCTCAATATGAGTCATTGCCGGCTCGGAAAGGGCATTTTTCTATCAGGCGGTGTTTTGGCTACTCCTATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0016740 GO:0016757 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

442

Amino Acids

49.25

Weight (kDa)

8.8

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 1 - 142 1.7e-30 Glycosyltransferase family 28 N-terminal domain
UDPGT PF00201 252 - 393 7e-06 UDP-glucoronosyl and UDP-glucosyl transferase
EryCIII-like_C PF06722 299 - 398 6e-13 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 344, 1031
AccII CGCG 1 cut(s) 19
AciI CCGC 1 cut(s) 1303
AclWI GGATC 3 cut(s) 149, 281, 1142
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 3 cut(s) 100, 422, 507
AcuI CTGAAG 1 cut(s) 566
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 5 cut(s) 16, 559, 586, 905, 960
AfiI CCNNNNNNNGG 5 cut(s) 149, 344, 930, 1031, 1049
AgsI TTSAA 7 cut(s) 106, 699, 785, 818, 997, 1115, 1175
AluBI AGCT 5 cut(s) 550, 740, 821, 1001, 1256
AluI AGCT 5 cut(s) 550, 740, 821, 1001, 1256
Alw26I GTCTC 2 cut(s) 507, 547
AlwI GGATC 3 cut(s) 149, 281, 1142
AoxI GGCC 3 cut(s) 70, 868, 1224
ApeKI GCWGC 2 cut(s) 986, 1001
ApoI RAATTY 3 cut(s) 100, 422, 507
ArsI GACNNNNNNTTYG 4 cut(s) 99, 131, 165, 197
AseI ATTAAT 1 cut(s) 494
AspS9I GGNCC 3 cut(s) 209, 653, 1079
AsuHPI GGTGA 2 cut(s) 755, 1064
AsuNHI GCTAGC 1 cut(s) 85
AvaII GGWCC 3 cut(s) 209, 653, 1079
BaeGI GKGCMC 1 cut(s) 351
BaeI ACNNNNGTAYC 2 cut(s) 568, 601
BalI TGGCCA 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 973, 988
BccI CCATC 2 cut(s) 211, 1196
BceAI ACGGC 1 cut(s) 938
BcgI CGANNNNNNTGC 2 cut(s) 1226, 1260
BciVI GTATCC 1 cut(s) 569
BcoDI GTCTC 2 cut(s) 507, 547
BfaI CTAG 2 cut(s) 86, 503
BfmI CTRYAG 1 cut(s) 580
BfuI GTATCC 1 cut(s) 569
BisI GCNGC 2 cut(s) 987, 1002
BlsI GCNGC 2 cut(s) 988, 1003
BmcAI AGTACT 1 cut(s) 586
Bme18I GGWCC 3 cut(s) 209, 653, 1079
BmgT120I GGNCC 3 cut(s) 209, 653, 1079
BmiI GGNNCC 3 cut(s) 210, 282, 654
BmrI ACTGGG 1 cut(s) 1088
BmsI GCATC 4 cut(s) 307, 478, 935, 1117
BmtI GCTAGC 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 1088
Bpu10I CCTNAGC 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 410
BsaJI CCNNGG 3 cut(s) 404, 871, 954
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 344, 930, 1031, 1049
Bse118I RCCGGY 1 cut(s) 1273
Bse1I ACTGG 3 cut(s) 417, 838, 1094
Bse3DI GCAATG 2 cut(s) 1194, 1268
BseDI CCNNGG 3 cut(s) 404, 871, 954
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 5 cut(s) 149, 344, 930, 1031, 1049
BseMI GCAATG 2 cut(s) 1194, 1268
BseMII CTCAG 2 cut(s) 354, 542
BseNI ACTGG 3 cut(s) 417, 838, 1094
BseRI GAGGAG 3 cut(s) 36, 579, 1086
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 2 cut(s) 973, 988
BseYI CCCAGC 3 cut(s) 125, 861, 974
BsgI GTGCAG 1 cut(s) 1068
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 3 cut(s) 72, 870, 1226
BsiSI CCGG 2 cut(s) 1212, 1274
BslFI GGGAC 3 cut(s) 222, 287, 991
BslI CCNNNNNNNGG 5 cut(s) 149, 344, 930, 1031, 1049
BsmAI GTCTC 2 cut(s) 507, 547
BsmBI CGTCTC 1 cut(s) 547
BsmFI GGGAC 3 cut(s) 222, 287, 991
BsnI GGCC 3 cut(s) 72, 870, 1226
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 5 cut(s) 6, 154, 286, 1036, 1147
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 1 cut(s) 1303
BspANI GGCC 3 cut(s) 72, 870, 1226
BspCNI CTCAG 2 cut(s) 355, 543
BspFNI CGCG 1 cut(s) 19
BspHI TCATGA 1 cut(s) 1325
BspLI GGNNCC 3 cut(s) 210, 282, 654
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 3 cut(s) 149, 281, 1142
BspQI GCTCTTC 1 cut(s) 941
BsrDI GCAATG 2 cut(s) 1194, 1268
BsrFI RCCGGY 1 cut(s) 1273
BsrI ACTGG 3 cut(s) 417, 838, 1094
BssAI RCCGGY 1 cut(s) 1273
BssECI CCNNGG 3 cut(s) 404, 871, 954
BssMI GATC 5 cut(s) 6, 154, 286, 1036, 1147
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 2 cut(s) 404, 871
Bst4CI ACNGT 1 cut(s) 1137
Bst6I CTCTTC 2 cut(s) 786, 941
BstACI GRCGYC 1 cut(s) 410
BstAPI GCANNNNNTGC 2 cut(s) 95, 326
BstC8I GCNNGC 4 cut(s) 87, 1065, 1167, 1275
BstDEI CTNAG 4 cut(s) 363, 551, 657, 907
BstDSI CCRYGG 2 cut(s) 404, 954
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 19
BstKTI GATC 5 cut(s) 9, 157, 289, 1039, 1150
BstMAI GTCTC 2 cut(s) 507, 547
BstMBI GATC 5 cut(s) 6, 154, 286, 1036, 1147
BstMWI GCNNNNNNNGC 5 cut(s) 95, 326, 409, 867, 1223
BstNSI RCATGY 1 cut(s) 1067
BstSFI CTRYAG 1 cut(s) 580
BstSLI GKGCMC 1 cut(s) 351
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 973, 988
BstX2I RGATCY 2 cut(s) 154, 1147
BstXI CCANNNNNNTGG 1 cut(s) 1189
BstYI RGATCY 2 cut(s) 154, 1147
BsuI GTATCC 1 cut(s) 569
BsuRI GGCC 3 cut(s) 72, 870, 1226
BtgI CCRYGG 2 cut(s) 404, 954
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 1224
BtsIMutI CAGTG 2 cut(s) 424, 1224
Cac8I GCNNGC 4 cut(s) 87, 1065, 1167, 1275
CciI TCATGA 1 cut(s) 1325
Cfr10I RCCGGY 1 cut(s) 1273
Cfr13I GGNCC 3 cut(s) 209, 653, 1079
CseI GACGC 1 cut(s) 418
Csp6I GTAC 5 cut(s) 15, 558, 585, 904, 959
CviAII CATG 9 cut(s) 95, 356, 405, 517, 926, 965, 1064, 1141, 1326
CviQI GTAC 5 cut(s) 15, 558, 585, 904, 959
DdeI CTNAG 4 cut(s) 363, 551, 657, 907
DpnI GATC 5 cut(s) 8, 156, 288, 1038, 1149
DpnII GATC 5 cut(s) 6, 154, 286, 1036, 1147
DraI TTTAAA 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 2 cut(s) 786, 941
EarI CTCTTC 2 cut(s) 786, 941
Eco130I CCWWGG 2 cut(s) 404, 871
Eco147I AGGCCT 1 cut(s) 1226
Eco47I GGWCC 3 cut(s) 209, 653, 1079
Eco57I CTGAAG 1 cut(s) 566
EcoO109I RGGNCCY 3 cut(s) 209, 653, 1079
EcoRI GAATTC 2 cut(s) 422, 507
EcoT14I CCWWGG 2 cut(s) 404, 871
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 2 cut(s) 404, 871
Esp3I CGTCTC 1 cut(s) 547
FaeI CATG 9 cut(s) 98, 359, 408, 520, 929, 968, 1067, 1144, 1329
FaqI GGGAC 3 cut(s) 222, 287, 991
FatI CATG 9 cut(s) 94, 355, 404, 516, 925, 964, 1063, 1140, 1325
FauNDI CATATG 1 cut(s) 343
Fnu4HI GCNGC 2 cut(s) 987, 1002
FokI GGATG 1 cut(s) 417
Fsp4HI GCNGC 2 cut(s) 987, 1002
FspBI CTAG 2 cut(s) 86, 503
GluI GCNGC 2 cut(s) 987, 1002
GsaI CCCAGC 3 cut(s) 129, 865, 978
HaeIII GGCC 3 cut(s) 72, 870, 1226
HapII CCGG 2 cut(s) 1212, 1274
HgaI GACGC 1 cut(s) 418
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 9 cut(s) 98, 359, 408, 520, 929, 968, 1067, 1144, 1329
HinfI GANTC 6 cut(s) 293, 382, 619, 716, 896, 1265
HpaII CCGG 2 cut(s) 1212, 1274
HphI GGTGA 2 cut(s) 755, 1064
Hpy166II GTNNAC 3 cut(s) 746, 904, 913
Hpy188I TCNGA 3 cut(s) 159, 217, 1281
Hpy8I GTNNAC 3 cut(s) 746, 904, 913
HpyAV CCTTC 3 cut(s) 222, 785, 1216
HpyCH4III ACNGT 1 cut(s) 1137
HpyCH4IV ACGT 2 cut(s) 556, 597
HpyCH4V TGCA 6 cut(s) 61, 98, 320, 491, 1063, 1085
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 326, 409, 867, 1223
HpyF3I CTNAG 4 cut(s) 363, 551, 657, 907
HpySE526I ACGT 2 cut(s) 556, 597
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 9 cut(s) 98, 359, 408, 520, 929, 968, 1067, 1144, 1329
KroI GCCGGC 1 cut(s) 1273
KroNI GCCGGC 1 cut(s) 1275
Kzo9I GATC 5 cut(s) 6, 154, 286, 1036, 1147
LguI GCTCTTC 1 cut(s) 941
LmnI GCTCC 2 cut(s) 737, 1214
Lsp1109I GCAGC 2 cut(s) 973, 988
LweI GCATC 4 cut(s) 307, 478, 935, 1117
MaeI CTAG 2 cut(s) 86, 503
MaeII ACGT 2 cut(s) 556, 597
MalI GATC 5 cut(s) 8, 156, 288, 1038, 1149
MboI GATC 5 cut(s) 6, 154, 286, 1036, 1147
MboII GAAGA 7 cut(s) 385, 547, 773, 806, 808, 928, 1211
MfeI CAATTG 1 cut(s) 916
MflI RGATCY 2 cut(s) 154, 1147
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 72
MluNI TGGCCA 1 cut(s) 72
MlyI GAGTC 2 cut(s) 628, 1274
MmeI TCCRAC 3 cut(s) 111, 742, 1034
Mox20I TGGCCA 1 cut(s) 72
Mph1103I ATGCAT 1 cut(s) 493
MroNI GCCGGC 1 cut(s) 1273
MscI TGGCCA 1 cut(s) 72
MseI TTAA 4 cut(s) 309, 494, 569, 1158
MslI CAYNNNNRTG 2 cut(s) 354, 969
Msp20I TGGCCA 1 cut(s) 72
MspI CCGG 2 cut(s) 1212, 1274
MunI CAATTG 1 cut(s) 916
MvnI CGCG 1 cut(s) 19
MwoI GCNNNNNNNGC 5 cut(s) 95, 326, 409, 867, 1223
NaeI GCCGGC 1 cut(s) 1275
NcoI CCATGG 1 cut(s) 404
NdeI CATATG 1 cut(s) 343
NdeII GATC 5 cut(s) 6, 154, 286, 1036, 1147
NgoMIV GCCGGC 1 cut(s) 1273
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 9 cut(s) 98, 359, 408, 520, 929, 968, 1067, 1144, 1329
NlaIV GGNNCC 3 cut(s) 210, 282, 654
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 1 cut(s) 1067
PaeI GCATGC 1 cut(s) 1067
PagI TCATGA 1 cut(s) 1325
PceI AGGCCT 1 cut(s) 1226
PciSI GCTCTTC 1 cut(s) 941
PdiI GCCGGC 1 cut(s) 1275
PfeI GAWTC 4 cut(s) 293, 382, 716, 896
PflMI CCANNNNNTGG 2 cut(s) 344, 1031
PkrI GCNGC 2 cut(s) 988, 1003
PleI GAGTC 2 cut(s) 627, 1273
PpsI GAGTC 2 cut(s) 627, 1273
PpuMI RGGWCCY 3 cut(s) 209, 653, 1079
PshBI ATTAAT 1 cut(s) 494
Psp5II RGGWCCY 3 cut(s) 209, 653, 1079
PspFI CCCAGC 3 cut(s) 125, 861, 974
PspN4I GGNNCC 3 cut(s) 210, 282, 654
PspPI GGNCC 3 cut(s) 209, 653, 1079
PspPPI RGGWCCY 3 cut(s) 209, 653, 1079
PsuI RGATCY 2 cut(s) 154, 1147
RsaI GTAC 5 cut(s) 16, 559, 586, 905, 960
RsaNI GTAC 5 cut(s) 15, 558, 585, 904, 959
RseI CAYNNNNRTG 2 cut(s) 354, 969
SapI GCTCTTC 1 cut(s) 941
SaqAI TTAA 4 cut(s) 309, 494, 569, 1158
SatI GCNGC 2 cut(s) 987, 1002
Sau3AI GATC 5 cut(s) 6, 154, 286, 1036, 1147
Sau96I GGNCC 3 cut(s) 209, 653, 1079
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 2 cut(s) 628, 1274
SduI GDGCHC 1 cut(s) 351
SfaNI GCATC 4 cut(s) 307, 478, 935, 1117
SfcI CTRYAG 1 cut(s) 580
SinI GGWCC 3 cut(s) 209, 653, 1079
SmiMI CAYNNNNRTG 2 cut(s) 354, 969
SphI GCATGC 1 cut(s) 1067
SseBI AGGCCT 1 cut(s) 1226
SsiI CCGC 1 cut(s) 1303
SspI AATATT 2 cut(s) 255, 808
SspMI CTAG 2 cut(s) 86, 503
StuI AGGCCT 1 cut(s) 1226
StyI CCWWGG 2 cut(s) 404, 871
TaaI ACNGT 1 cut(s) 1137
TaiI ACGT 2 cut(s) 559, 600
TaqI TCGA 2 cut(s) 486, 511
TatI WGTACW 2 cut(s) 584, 903
TfiI GAWTC 4 cut(s) 293, 382, 716, 896
Tru1I TTAA 4 cut(s) 309, 494, 569, 1158
Tru9I TTAA 4 cut(s) 309, 494, 569, 1158
TscAI CASTG 2 cut(s) 424, 1224
TseI GCWGC 2 cut(s) 986, 1001
TspDTI ATGAA 6 cut(s) 374, 720, 1129, 1212, 1220, 1314
TspRI CASTG 2 cut(s) 424, 1224
Van91I CCANNNNNTGG 2 cut(s) 344, 1031
VpaK11BI GGWCC 3 cut(s) 209, 653, 1079
VspI ATTAAT 1 cut(s) 494
XapI RAATTY 3 cut(s) 100, 422, 507
XceI RCATGY 1 cut(s) 1067
XspI CTAG 2 cut(s) 86, 503
ZrmI AGTACT 1 cut(s) 586
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.