FvH4_2g21940

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004582
Physical Location & Seq
Forward (+)
18115174 .. 18116229
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21940.t1

Sequence Viewer

Length: 564 bp
ATGACAGGGTCTGCTGGGTCTCCTTGTGGAGCCTGCAAGTTCTTGAGAAGAAAATGTGTCAGGGGTTGCATTTTTGCACCTTATTTCTGCCATGAACAAGGCGCTACACATTTTGCAGCCATCCACAGGGTTTTTGGGGCGAGCAATGTGTCTAAATTGCTTGCGCAACTCCCCATTAATGATCGTTGCAGTGCTGCGCTTACAATTTCGTATGAAGCTCAAGCCAGGGTTCAAGATCCTGTTTATGGCTGTGTTTCCCATATTTTTGCTCTCCAACAACAGGTTATTAATCTACAAGCACAACTAGCTTCAGCCAAGGAACAAGCATCTAAAAGCAATTCCACTGGCTCTGACAATTCATTGAACCCAAGTTGGCTTCAGTACTCAGAAAATTTAAACATGATGAATCAGCAACATCCTAACTGTTTCGGGAATGACGAAAATTTCATGGTTCCTGAACTTCTAAATGACATATATGGAAGGTTTGGGGAGTCTTCTAATTCCCTGAGCTCATTAGATCACATCCAAACAAACTGTATGCAATGGAGTTTTGATCAAGAGTAA

Protein Analysis

188

Amino Acids

20.66

Weight (kDa)

6.04

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 8 - 106 1e-34 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 165
AclWI GGATC 1 cut(s) 230
AcsI RAATTY 2 cut(s) 391, 442
AcuI CTGAAG 2 cut(s) 294, 362
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 3 cut(s) 126, 245, 280
AgsI TTSAA 2 cut(s) 233, 364
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 3 cut(s) 218, 308, 510
AluI AGCT 3 cut(s) 218, 308, 510
Alw21I GWGCWC 1 cut(s) 512
Alw26I GTCTC 1 cut(s) 24
AlwI GGATC 1 cut(s) 230
AlwNI CAGNNNCTG 1 cut(s) 11
ApeKI GCWGC 2 cut(s) 116, 194
ApoI RAATTY 2 cut(s) 391, 442
AseI ATTAAT 2 cut(s) 177, 288
AspLEI GCGC 3 cut(s) 104, 166, 199
BanII GRGCYC 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 2 cut(s) 128, 181
BccI CCATC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 226
BclI TGATCA 1 cut(s) 553
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 105
BisI GCNGC 2 cut(s) 117, 195
BlsI GCNGC 2 cut(s) 118, 196
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 226
BmiI GGNNCC 2 cut(s) 31, 453
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 506
BpuEI CTTGAG 2 cut(s) 64, 204
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 225, 315
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 245, 280
Bse1I ACTGG 1 cut(s) 349
Bse3DI GCAATG 2 cut(s) 151, 548
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 225, 315
BseGI GGATG 3 cut(s) 120, 415, 522
BseLI CCNNNNNNNGG 3 cut(s) 126, 245, 280
BseMI GCAATG 2 cut(s) 151, 548
BseMII CTCAG 2 cut(s) 399, 497
BseNI ACTGG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 128, 181
BseYI CCCAGC 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 512
BslI CCNNNNNNNGG 3 cut(s) 126, 245, 280
BsmAI GTCTC 1 cut(s) 24
Bso31I GGTCTC 1 cut(s) 24
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 4 cut(s) 181, 235, 517, 553
BspCNI CTCAG 2 cut(s) 398, 498
BspLI GGNNCC 2 cut(s) 31, 453
BspPI GGATC 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 24
BsrDI GCAATG 2 cut(s) 151, 548
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 225, 315
BssMI GATC 4 cut(s) 181, 235, 517, 553
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 2 cut(s) 425, 536
BstC8I GCNNGC 3 cut(s) 34, 142, 162
BstDEI CTNAG 2 cut(s) 385, 506
BstF5I GGATG 3 cut(s) 120, 415, 522
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 3 cut(s) 104, 166, 199
BstKTI GATC 4 cut(s) 184, 238, 520, 556
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 4 cut(s) 181, 235, 517, 553
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 128, 181
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BtsCI GGATG 3 cut(s) 120, 415, 522
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 2 cut(s) 196, 342
Cac8I GCNNGC 3 cut(s) 34, 142, 162
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 3 cut(s) 104, 166, 199
Csp6I GTAC 1 cut(s) 382
CviAII CATG 3 cut(s) 92, 400, 448
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 2 cut(s) 385, 506
DpnI GATC 4 cut(s) 183, 237, 519, 555
DpnII GATC 4 cut(s) 181, 235, 517, 553
DraI TTTAAA 1 cut(s) 396
Ecl136II GAGCTC 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 512
Eco31I GGTCTC 1 cut(s) 24
Eco53kI GAGCTC 1 cut(s) 510
Eco57I CTGAAG 2 cut(s) 294, 362
EcoICRI GAGCTC 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 3 cut(s) 95, 403, 451
FatI CATG 3 cut(s) 91, 399, 447
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 2 cut(s) 117, 195
FokI GGATG 3 cut(s) 107, 402, 509
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 2 cut(s) 117, 195
FspBI CTAG 1 cut(s) 305
FspI TGCGCA 1 cut(s) 165
GlaI GCGC 3 cut(s) 103, 165, 198
GluI GCNGC 2 cut(s) 117, 195
GsaI CCCAGC 1 cut(s) 18
HaeII RGCGCY 1 cut(s) 105
HhaI GCGC 3 cut(s) 104, 166, 199
Hin1II CATG 3 cut(s) 95, 403, 451
Hin6I GCGC 3 cut(s) 102, 164, 197
HinP1I GCGC 3 cut(s) 102, 164, 197
HinfI GANTC 2 cut(s) 406, 491
Hpy188I TCNGA 2 cut(s) 352, 388
Hpy188III TCNNGA 5 cut(s) 43, 233, 430, 455, 557
HpyAV CCTTC 1 cut(s) 474
HpyCH4III ACNGT 2 cut(s) 425, 536
HpyCH4V TGCA 6 cut(s) 36, 69, 77, 116, 189, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 2 cut(s) 385, 506
Hsp92II CATG 3 cut(s) 95, 403, 451
HspAI GCGC 3 cut(s) 102, 164, 197
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 4 cut(s) 181, 235, 517, 553
LmnI GCTCC 1 cut(s) 29
Lsp1109I GCAGC 2 cut(s) 128, 181
LweI GCATC 1 cut(s) 335
MaeI CTAG 1 cut(s) 305
MalI GATC 4 cut(s) 183, 237, 519, 555
MboI GATC 4 cut(s) 181, 235, 517, 553
MboII GAAGA 2 cut(s) 60, 486
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 512
MluCI AATT 7 cut(s) 155, 204, 337, 355, 391, 442, 499
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 298
MseI TTAA 3 cut(s) 177, 288, 395
MspR9I CCNGG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeII GATC 4 cut(s) 181, 235, 517, 553
NlaIII CATG 3 cut(s) 95, 403, 451
NlaIV GGNNCC 2 cut(s) 31, 453
NsbI TGCGCA 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PfeI GAWTC 1 cut(s) 406
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 2 cut(s) 118, 196
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PshBI ATTAAT 2 cut(s) 177, 288
Psp124BI GAGCTC 1 cut(s) 512
Psp6I CCWGG 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 2 cut(s) 31, 453
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SacI GAGCTC 1 cut(s) 512
SaqAI TTAA 3 cut(s) 177, 288, 395
SatI GCNGC 2 cut(s) 117, 195
Sau3AI GATC 4 cut(s) 181, 235, 517, 553
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 1 cut(s) 512
SetI ASST 6 cut(s) 82, 220, 285, 310, 485, 512
SfaNI GCATC 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 43, 219
SmoI CTYRAG 2 cut(s) 43, 219
Sse9I AATT 7 cut(s) 155, 204, 337, 355, 391, 442, 499
SspMI CTAG 1 cut(s) 305
SstI GAGCTC 1 cut(s) 512
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 2 cut(s) 425, 536
TasI AATT 7 cut(s) 155, 204, 337, 355, 391, 442, 499
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 3 cut(s) 177, 288, 395
Tru9I TTAA 3 cut(s) 177, 288, 395
TscAI CASTG 2 cut(s) 196, 349
TseI GCWGC 2 cut(s) 116, 194
TspDTI ATGAA 5 cut(s) 108, 228, 348, 419, 436
TspRI CASTG 2 cut(s) 196, 349
Tth111I GACNNNGTC 1 cut(s) 7
VspI ATTAAT 2 cut(s) 177, 288
XapI RAATTY 2 cut(s) 391, 442
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XspI CTAG 1 cut(s) 305
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.