MD05G1200800.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004582
Physical Location & Seq
Forward (+)
32813649 .. 32814766
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1200800.v1.1.491

Sequence Viewer

Length: 720 bp
ATGACTGGTTCTGGTTCTCCTTGCGGAGCCTGCAAGTTCTTGAGAAGAAAATGTGTAAGAGGGTGTATTTTTGCACCTTACTTCTGCCATGAACAAGGTGCTACCCATTTCTCGGCCATCCACAAGGTTTTTGGCGCAAGCAATGCGTCGAAGCTGCTTGCTCACCTTCCGGTGGGTGATCGTTGTGAAGCCGCCATGACAATATCATATGAAGCTCAAGCCAGGCTTCAAGATCCCATTTATGGTTGTGTTTCTCATATTTTTTCTCTCCAACAGCAGGTGGTCAGCTTACAAGCACAATTAGATTCCCTCAAGGATCAAGCAGCTCAAAGCTTTCTCAAGGGCTCTGCTGTAGCAAACCCTAATGAGAAATTTTATGGGAAAGCTCCTTCTCATACAGAAGTTCATAGTTGGTTCCATGAGCTAGAAAGTTCTTCAAACACTTTTCCACAATTCAATTCAAATCAAACCAATGATGCTGGAACCACATCATACTATGAGAATGGAATGATGAACTCAAATAACTCTGTTAATGGGAGTCATGAAAATTCAGTGAATATCCCAGATGAAAATGTCTCCTCATATGGCAGCTTTGAAGAGGGCACTTCTCATTCCATGTCTTCTTTTGACGTGCAAACCAATTTCAACAAGCAATGGGGTTTTCATGATGCTAATGAGCTTCAATCAGTGGCTTTCAGCTACATTGACCAGCATTCATGA

Protein Analysis

240

Amino Acids

26.26

Weight (kDa)

5.79

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 7 - 105 9.1e-34 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 268
Acc36I ACCTGC 1 cut(s) 268
AciI CCGC 2 cut(s) 24, 192
AclWI GGATC 2 cut(s) 227, 324
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 2 cut(s) 371, 547
AfiI CCNNNNNNNGG 4 cut(s) 112, 172, 242, 277
AgsI TTSAA 7 cut(s) 230, 438, 457, 462, 596, 646, 683
AjiI CACGTC 1 cut(s) 631
AjnI CCWGG 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 580
AlwI GGATC 2 cut(s) 227, 324
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 3 cut(s) 154, 323, 588
ApoI RAATTY 2 cut(s) 371, 547
AspLEI GCGC 1 cut(s) 137
AsuHPI GGTGA 2 cut(s) 155, 188
BaeGI GKGCMC 1 cut(s) 605
BanII GRGCYC 1 cut(s) 347
BbsI GAAGAC 1 cut(s) 612
BbvI GCAGC 3 cut(s) 141, 335, 600
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 580
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 4 cut(s) 155, 192, 324, 589
BlsI GCNGC 4 cut(s) 156, 193, 325, 590
Bme1390I CCNGG 1 cut(s) 223
BmgBI CACGTC 1 cut(s) 631
BmiI GGNNCC 3 cut(s) 28, 416, 484
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 2 cut(s) 466, 658
BpiI GAAGAC 1 cut(s) 612
BpuEI CTTGAG 4 cut(s) 61, 201, 296, 323
BsaWI WCCGGW 1 cut(s) 169
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 172, 242, 277
Bse1I ACTGG 1 cut(s) 10
Bse3DI GCAATG 2 cut(s) 148, 659
BseBI CCWGG 1 cut(s) 223
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 4 cut(s) 112, 172, 242, 277
BseMI GCAATG 2 cut(s) 148, 659
BseNI ACTGG 1 cut(s) 10
BseRI GAGGAG 1 cut(s) 568
BseSI GKGCMC 1 cut(s) 605
BseXI GCAGC 3 cut(s) 141, 335, 600
BshFI GGCC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 170
BslI CCNNNNNNNGG 4 cut(s) 112, 172, 242, 277
BsmAI GTCTC 1 cut(s) 580
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 347, 605
Bsp143I GATC 3 cut(s) 178, 232, 316
BspACI CCGC 2 cut(s) 24, 192
BspANI GGCC 1 cut(s) 116
BspHI TCATGA 3 cut(s) 541, 664, 716
BspLI GGNNCC 3 cut(s) 28, 416, 484
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 2 cut(s) 227, 324
BsrDI GCAATG 2 cut(s) 148, 659
BsrI ACTGG 1 cut(s) 10
BssMI GATC 3 cut(s) 178, 232, 316
Bst2UI CCWGG 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 591
BstAPI GCANNNNNTGC 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 31, 139, 159
BstF5I GGATG 1 cut(s) 117
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 3 cut(s) 181, 235, 319
BstMAI GTCTC 1 cut(s) 580
BstMBI GATC 3 cut(s) 178, 232, 316
BstMWI GCNNNNNNNGC 2 cut(s) 30, 143
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 605
BstV1I GCAGC 3 cut(s) 141, 335, 600
BstV2I GAAGAC 1 cut(s) 612
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BsuRI GGCC 1 cut(s) 116
BtrI CACGTC 1 cut(s) 631
BtsCI GGATG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 558, 693
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 3 cut(s) 31, 139, 159
CciI TCATGA 3 cut(s) 541, 664, 716
CfoI GCGC 1 cut(s) 137
CseI GACGC 1 cut(s) 135
CviAII CATG 7 cut(s) 89, 196, 419, 542, 616, 665, 717
DpnI GATC 3 cut(s) 180, 234, 318
DpnII GATC 3 cut(s) 178, 232, 316
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 347
FaeI CATG 7 cut(s) 92, 199, 422, 545, 619, 668, 720
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FatI CATG 7 cut(s) 88, 195, 418, 541, 615, 664, 716
FauNDI CATATG 2 cut(s) 208, 583
Fnu4HI GCNGC 4 cut(s) 155, 192, 324, 589
FokI GGATG 1 cut(s) 104
FriOI GRGCYC 1 cut(s) 347
Fsp4HI GCNGC 4 cut(s) 155, 192, 324, 589
FspBI CTAG 1 cut(s) 425
GlaI GCGC 1 cut(s) 136
GluI GCNGC 4 cut(s) 155, 192, 324, 589
HaeIII GGCC 1 cut(s) 116
HapII CCGG 1 cut(s) 170
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 137
Hin1II CATG 7 cut(s) 92, 199, 422, 545, 619, 668, 720
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HindIII AAGCTT 1 cut(s) 331
HinfI GANTC 2 cut(s) 305, 538
HpaII CCGG 1 cut(s) 170
HphI GGTGA 2 cut(s) 155, 188
Hpy188III TCNNGA 5 cut(s) 40, 230, 542, 665, 717
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 176, 399
HpyCH4IV ACGT 1 cut(s) 630
HpyCH4V TGCA 3 cut(s) 33, 74, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 143
HpySE526I ACGT 1 cut(s) 630
Hsp92II CATG 7 cut(s) 92, 199, 422, 545, 619, 668, 720
HspAI GCGC 1 cut(s) 135
Kzo9I GATC 3 cut(s) 178, 232, 316
LmnI GCTCC 2 cut(s) 26, 391
LpnPI CCDG 7 cut(s) 43, 183, 208, 235, 263, 465, 576
Lsp1109I GCAGC 3 cut(s) 141, 335, 600
LweI GCATC 2 cut(s) 466, 658
MaeI CTAG 1 cut(s) 425
MaeII ACGT 1 cut(s) 630
MalI GATC 3 cut(s) 180, 234, 318
MboI GATC 3 cut(s) 178, 232, 316
MboII GAAGA 4 cut(s) 57, 426, 608, 612
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 2 cut(s) 347, 605
MluCI AATT 6 cut(s) 299, 371, 452, 457, 547, 640
MlyI GAGTC 1 cut(s) 547
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 4 cut(s) 53, 320, 589, 592
MseI TTAA 1 cut(s) 531
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 223
Mva1269I GAATGC 1 cut(s) 712
MvaI CCWGG 1 cut(s) 223
MwoI GCNNNNNNNGC 2 cut(s) 30, 143
NdeI CATATG 2 cut(s) 208, 583
NdeII GATC 3 cut(s) 178, 232, 316
NlaIII CATG 7 cut(s) 92, 199, 422, 545, 619, 668, 720
NlaIV GGNNCC 3 cut(s) 28, 416, 484
NmeAIII GCCGAG 1 cut(s) 92
PagI TCATGA 3 cut(s) 541, 664, 716
PaqCI CACCTGC 1 cut(s) 268
PctI GAATGC 1 cut(s) 712
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 4 cut(s) 156, 193, 325, 590
PleI GAGTC 1 cut(s) 546
PpsI GAGTC 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 3 cut(s) 28, 416, 484
PsuI RGATCY 1 cut(s) 232
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 4 cut(s) 155, 192, 324, 589
Sau3AI GATC 3 cut(s) 178, 232, 316
SchI GAGTC 1 cut(s) 547
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 2 cut(s) 347, 605
SfaNI GCATC 2 cut(s) 466, 658
SfcI CTRYAG 1 cut(s) 351
SmlI CTYRAG 4 cut(s) 40, 216, 311, 338
SmoI CTYRAG 4 cut(s) 40, 216, 311, 338
Sse9I AATT 6 cut(s) 299, 371, 452, 457, 547, 640
SsiI CCGC 2 cut(s) 24, 192
SspMI CTAG 1 cut(s) 425
StyD4I CCNGG 1 cut(s) 221
TaiI ACGT 1 cut(s) 633
TaqI TCGA 1 cut(s) 149
TasI AATT 6 cut(s) 299, 371, 452, 457, 547, 640
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 2 cut(s) 558, 693
TseI GCWGC 3 cut(s) 154, 323, 588
TspDTI ATGAA 8 cut(s) 105, 225, 395, 527, 558, 582, 653, 705
TspRI CASTG 2 cut(s) 558, 693
XapI RAATTY 2 cut(s) 371, 547
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.