Rroxscaffold_7G00179860

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0004582
Physical Location & Seq
Reverse (-)
19336020 .. 19336675
656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179860.1

Sequence Viewer

Length: 564 bp
ATGGCAGGGTCTGCCGGTTCTCCTTGTGGAGCCTGCAAGTTCCTGAGAAGAAAATGTGCCAGGGGTTGCATTTTTGCACCTTATTTCTGCCATGAACAAGGTGCTACACATTTTGCAGCCATCCACAAGGTTTTCGGAGCAAGCAATGTGTCGAAATTGCTTGCTCAACTCCCCATTAATGATCGTTGCAGAGCTGTGCTTACAATTTCGTATGAAGCTCAAGCCAGGGTTCAAGATCCTATTTATGGCTGTGTTTCCCATATTTTTGCTCTCCAACAACAGGTTATTAATCTACAGGCACAACTAGCTTCAGCCAAGGAACAAGCAGCTAAAAGCAATTCCACTGGCTCTGACAATTCAATGAACCCAAGTTGGCTTCAGTACTCGGAAAATTTAAACATGACGAATCAGCAACATCCCATCTCTTTCGGGAATGACGAAAATTTCATGGTTCCAGAAGTACTAAATGACATATATGGAGGCTTTGGGGAGTCTGCTAATTCCATGAGCTCATTAGATCACATGCAAACAAACAGTATGCAGTGGAGTTTTGATCAAGCCTAA

Protein Analysis

187

Amino Acids

20.48

Weight (kDa)

6.27

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 8 - 106 7.1e-35 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 230
AcsI RAATTY 2 cut(s) 391, 442
AcuI CTGAAG 2 cut(s) 294, 362
AfaI GTAC 2 cut(s) 383, 462
AfiI CCNNNNNNNGG 2 cut(s) 245, 280
AgsI TTSAA 2 cut(s) 233, 360
AjnI CCWGG 2 cut(s) 59, 224
AluBI AGCT 5 cut(s) 194, 218, 308, 329, 510
AluI AGCT 5 cut(s) 194, 218, 308, 329, 510
Alw21I GWGCWC 1 cut(s) 512
AlwI GGATC 1 cut(s) 230
AlwNI CAGNNNCTG 1 cut(s) 11
ApeKI GCWGC 2 cut(s) 116, 326
ApoI RAATTY 2 cut(s) 391, 442
AseI ATTAAT 2 cut(s) 177, 288
BanII GRGCYC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 512
BbvI GCAGC 2 cut(s) 128, 338
BccI CCATC 2 cut(s) 128, 428
BciT130I CCWGG 2 cut(s) 61, 226
BclI TGATCA 1 cut(s) 553
BfaI CTAG 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 293
BisI GCNGC 2 cut(s) 117, 327
BlsI GCNGC 2 cut(s) 118, 328
BmcAI AGTACT 2 cut(s) 383, 462
Bme1390I CCNGG 2 cut(s) 61, 226
BmiI GGNNCC 2 cut(s) 31, 453
BmrFI CCNGG 2 cut(s) 61, 226
BpuEI CTTGAG 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 60, 225, 315
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 280
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 151
BseBI CCWGG 2 cut(s) 61, 226
BseDI CCNNGG 3 cut(s) 60, 225, 315
BseGI GGATG 2 cut(s) 120, 415
BseLI CCNNNNNNNGG 2 cut(s) 245, 280
BseMI GCAATG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 1 cut(s) 349
BseXI GCAGC 2 cut(s) 128, 338
BsiHKAI GWGCWC 1 cut(s) 512
BsiSI CCGG 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 245, 280
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 4 cut(s) 181, 235, 517, 553
BspCNI CTCAG 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 31, 453
BspPI GGATC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 14
BsrI ACTGG 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 3 cut(s) 60, 225, 315
BssMI GATC 4 cut(s) 181, 235, 517, 553
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 61, 226
Bst4CI ACNGT 1 cut(s) 536
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 34, 142, 162
BstDEI CTNAG 1 cut(s) 44
BstF5I GGATG 2 cut(s) 120, 415
BstKTI GATC 4 cut(s) 184, 238, 520, 556
BstMBI GATC 4 cut(s) 181, 235, 517, 553
BstMWI GCNNNNNNNGC 2 cut(s) 11, 305
BstNI CCWGG 2 cut(s) 61, 226
BstNSI RCATGY 1 cut(s) 526
BstSCI CCNGG 2 cut(s) 59, 224
BstSFI CTRYAG 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 128, 338
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BtsCI GGATG 2 cut(s) 120, 415
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 2 cut(s) 342, 548
Cac8I GCNNGC 3 cut(s) 34, 142, 162
CaiI CAGNNNCTG 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 14
Csp6I GTAC 2 cut(s) 382, 461
CviAII CATG 5 cut(s) 92, 400, 448, 505, 523
CviQI GTAC 2 cut(s) 382, 461
DdeI CTNAG 1 cut(s) 44
DpnI GATC 4 cut(s) 183, 237, 519, 555
DpnII GATC 4 cut(s) 181, 235, 517, 553
DraI TTTAAA 1 cut(s) 396
Ecl136II GAGCTC 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 512
Eco53kI GAGCTC 1 cut(s) 510
Eco57I CTGAAG 2 cut(s) 294, 362
EcoICRI GAGCTC 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 59, 224
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 5 cut(s) 95, 403, 451, 508, 526
FatI CATG 5 cut(s) 91, 399, 447, 504, 522
FbaI TGATCA 1 cut(s) 553
Fnu4HI GCNGC 2 cut(s) 117, 327
FokI GGATG 2 cut(s) 107, 402
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 2 cut(s) 117, 327
FspBI CTAG 1 cut(s) 305
GluI GCNGC 2 cut(s) 117, 327
HapII CCGG 1 cut(s) 15
Hin1II CATG 5 cut(s) 95, 403, 451, 508, 526
HinfI GANTC 2 cut(s) 406, 491
HpaII CCGG 1 cut(s) 15
Hpy188I TCNGA 3 cut(s) 137, 352, 388
Hpy188III TCNNGA 4 cut(s) 43, 233, 430, 455
HpyCH4III ACNGT 1 cut(s) 536
HpyCH4V TGCA 7 cut(s) 36, 69, 77, 116, 189, 526, 541
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 305
HpyF3I CTNAG 1 cut(s) 44
Hsp92II CATG 5 cut(s) 95, 403, 451, 508, 526
Ksp22I TGATCA 1 cut(s) 553
Kzo9I GATC 4 cut(s) 181, 235, 517, 553
LmnI GCTCC 2 cut(s) 29, 137
Lsp1109I GCAGC 2 cut(s) 128, 338
MaeI CTAG 1 cut(s) 305
MalI GATC 4 cut(s) 183, 237, 519, 555
MboI GATC 4 cut(s) 181, 235, 517, 553
MboII GAAGA 1 cut(s) 60
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 512
MluCI AATT 7 cut(s) 155, 204, 337, 355, 391, 442, 499
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 1 cut(s) 473
MseI TTAA 3 cut(s) 177, 288, 395
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 2 cut(s) 61, 226
MvaI CCWGG 2 cut(s) 61, 226
MwoI GCNNNNNNNGC 2 cut(s) 11, 305
NdeII GATC 4 cut(s) 181, 235, 517, 553
NlaIII CATG 5 cut(s) 95, 403, 451, 508, 526
NlaIV GGNNCC 2 cut(s) 31, 453
NspI RCATGY 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 2 cut(s) 118, 328
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PshBI ATTAAT 2 cut(s) 177, 288
Psp124BI GAGCTC 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 59, 224
PspGI CCWGG 2 cut(s) 59, 224
PspN4I GGNNCC 2 cut(s) 31, 453
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 235
RsaI GTAC 2 cut(s) 383, 462
RsaNI GTAC 2 cut(s) 382, 461
SacI GAGCTC 1 cut(s) 512
SaqAI TTAA 3 cut(s) 177, 288, 395
SatI GCNGC 2 cut(s) 117, 327
Sau3AI GATC 4 cut(s) 181, 235, 517, 553
ScaI AGTACT 2 cut(s) 383, 462
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 61, 226
SduI GDGCHC 1 cut(s) 512
SetI ASST 9 cut(s) 82, 103, 132, 196, 220, 285, 310, 331, 512
SfcI CTRYAG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 219
SmoI CTYRAG 1 cut(s) 219
Sse9I AATT 7 cut(s) 155, 204, 337, 355, 391, 442, 499
SspMI CTAG 1 cut(s) 305
SstI GAGCTC 1 cut(s) 512
StyD4I CCNGG 2 cut(s) 59, 224
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 536
TaqI TCGA 1 cut(s) 152
TasI AATT 7 cut(s) 155, 204, 337, 355, 391, 442, 499
TatI WGTACW 2 cut(s) 381, 460
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 3 cut(s) 177, 288, 395
Tru9I TTAA 3 cut(s) 177, 288, 395
TscAI CASTG 2 cut(s) 349, 548
TseI GCWGC 2 cut(s) 116, 326
TspDTI ATGAA 4 cut(s) 108, 228, 377, 436
TspRI CASTG 2 cut(s) 349, 548
VspI ATTAAT 2 cut(s) 177, 288
XapI RAATTY 2 cut(s) 391, 442
XceI RCATGY 1 cut(s) 526
XspI CTAG 1 cut(s) 305
ZrmI AGTACT 2 cut(s) 383, 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.