Rorug06G0198400

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004582
Physical Location & Seq
Reverse (-)
31940989 .. 31943668
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0198400.1

Sequence Viewer

Length: 1170 bp
ATGTCTCACAGGAAGTTTGAGCACCCCAGGCATGGATCCCTTGGATTTCTTCCAAGGAAGCGAGCTGCTCGTCACAGAGGAAAAGTGAAGGCTTTCCCCAAGGATGACCCATCCAAGCCCTGCAAGCTCACTGCTTTCCTTGGTTACAAGGCTGGGATGACTCACATTGTCAGGGATGTCGAGAAACCTGGATCAAAGCTGCACAAGAAGGAGACATGTGAGGCTGTCACTATCATTGAGGCTCCTCCAATGGTCGTTGTTGGTGTTGTGGGTTATGTGAAGACACCCCGCGGCCTTCGTTCTTTGAACACAGTGTGGGCTCAGCATCTCAGTGAGGAGGTTAAGAGGAGGTTTTACAAGAACTGGTGCAAGTCCAAGAAGAAGGCTTTCGCAAAGTACTCCAAGACCTATGAAACTGACGAAGGAAAGAAGAATATTGAAGGGCAGTTTGAGAAACTGATTAAATATGCTACTGTTATTCGAGTTTTGGCTCACACTCAGATCAGGAAGATGAAGGGTTTGAAGCAGAAGAAAGCACATCTCATGGAGATCCAGGTGAATGGTGGCACAATTGCTCAAAAGGTTGAATTTGCAAAAAGCTTCTTTGAGAAGCAAATCCCTATTGATGCCGTCTTCCAGAAAGATGAAATGATTGACATCATTGGTGTGACAAAGGGTAAGGGTTATGAAGGTGTTGTTACTCGTTGGGGTGTCACCCGGCTTCCCCGTAAGACCCATAGGGGTCTGCGTAAGGTTGCTTGTATTGGTGCATGGCATCCTGCCAGAGTTTCATTTACAGTTGCCAGGGCTGGTCAAAATGGATACCATCACCGTACTGAAATGAACAAGAAAATTTACAAGCTTGGAAAGGCCGGACAAGAGTCACACACTGCTGTCACTGAGTTTGACAGGACTGAGAAAGACATCACTCCAATTGGTGGCTTCCCTCATTATGGTGTGGTGAAAGATGACTACATTCTGATCAAGGGATGCTGTGTTGGACCCAAGAAGAGGGTTGTTACCCTTCGCCAGTCCCTGCTCAAGCAGACCTCTCGTCTTGCTCTTGAGGATATCAAGCTCAAGTTCATCGATACCTCCTCAAAGTTCGGGCATGGTCGCTTCCAGACTACACAGGAGAAACAGAAGTACTATGGACGACTCAAGGCATAA

Protein Analysis

389

Amino Acids

44.32

Weight (kDa)

10.14

Isoelectric Point (pI)

30.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 1 - 370 4.4e-195 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 938
AccII CGCG 1 cut(s) 291
AciI CCGC 2 cut(s) 289, 291
AclWI GGATC 4 cut(s) 30, 43, 199, 544
AcsI RAATTY 2 cut(s) 587, 852
AdeI CACNNNGTG 1 cut(s) 315
AfaI GTAC 3 cut(s) 398, 835, 1148
AfiI CCNNNNNNNGG 4 cut(s) 32, 938, 953, 1011
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 4 cut(s) 307, 440, 523, 587
AhdI GACNNNNNGTC 1 cut(s) 1053
AjnI CCWGG 4 cut(s) 26, 187, 552, 803
AluBI AGCT 6 cut(s) 65, 127, 199, 600, 862, 1078
AluI AGCT 6 cut(s) 65, 127, 199, 600, 862, 1078
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 2 cut(s) 9, 206
AlwI GGATC 4 cut(s) 30, 43, 199, 544
AlwNI CAGNNNCTG 1 cut(s) 1036
AoxI GGCC 2 cut(s) 292, 870
ApeKI GCWGC 2 cut(s) 65, 199
ApoI RAATTY 2 cut(s) 587, 852
Asp700I GAANNNNTTC 2 cut(s) 92, 386
AspS9I GGNCC 1 cut(s) 1001
AsuC2I CCSGG 1 cut(s) 718
AsuHPI GGTGA 4 cut(s) 568, 706, 821, 973
AvaII GGWCC 1 cut(s) 1001
BamHI GGATCC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 322
BbsI GAAGAC 2 cut(s) 287, 625
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 52, 186
BccI CCATC 2 cut(s) 118, 834
BceAI ACGGC 1 cut(s) 614
BcgI CGANNNNNNTGC 2 cut(s) 1034, 1068
BciT130I CCWGG 4 cut(s) 28, 189, 554, 805
BciVI GTATCC 1 cut(s) 815
BclI TGATCA 1 cut(s) 981
BcnI CCSGG 1 cut(s) 718
BcoDI GTCTC 2 cut(s) 9, 206
BfuI GTATCC 1 cut(s) 815
BisI GCNGC 3 cut(s) 66, 200, 292
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 3 cut(s) 67, 201, 293
BmcAI AGTACT 2 cut(s) 398, 1148
Bme1390I CCNGG 5 cut(s) 28, 189, 554, 718, 805
Bme18I GGWCC 1 cut(s) 1001
BmeRI GACNNNNNGTC 1 cut(s) 1053
BmgT120I GGNCC 1 cut(s) 1001
BmiI GGNNCC 3 cut(s) 37, 243, 1003
BmrFI CCNGG 5 cut(s) 28, 189, 554, 718, 805
BmsI GCATC 4 cut(s) 334, 616, 784, 980
BoxI GACNNNNGTC 1 cut(s) 880
BpiI GAAGAC 2 cut(s) 287, 625
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 4 cut(s) 1025, 1064, 1085, 1145
BpuMI CCSGG 1 cut(s) 718
Bsa29I ATCGAT 1 cut(s) 1089
BsaBI GATNNNNATC 1 cut(s) 656
BsaJI CCNNGG 7 cut(s) 26, 40, 53, 99, 139, 289, 804
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 938, 953, 1011
Bse1I ACTGG 2 cut(s) 368, 1030
Bse8I GATNNNNATC 1 cut(s) 656
BseBI CCWGG 4 cut(s) 28, 189, 554, 805
BseCI ATCGAT 1 cut(s) 1089
BseDI CCNNGG 7 cut(s) 26, 40, 53, 99, 139, 289, 804
BseGI GGATG 6 cut(s) 109, 110, 162, 181, 775, 995
BseJI GATNNNNATC 1 cut(s) 656
BseLI CCNNNNNNNGG 4 cut(s) 32, 938, 953, 1011
BseMII CTCAG 5 cut(s) 335, 343, 512, 891, 906
BseNI ACTGG 2 cut(s) 368, 1030
BseRI GAGGAG 4 cut(s) 234, 350, 361, 1087
BseXI GCAGC 2 cut(s) 52, 186
BseYI CCCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 185
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 2 cut(s) 294, 872
BshVI ATCGAT 1 cut(s) 1089
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 2 cut(s) 718, 873
BslFI GGGAC 1 cut(s) 1018
BslI CCNNNNNNNGG 4 cut(s) 32, 938, 953, 1011
BsmAI GTCTC 2 cut(s) 9, 206
BsmFI GGGAC 1 cut(s) 1018
BsnI GGCC 2 cut(s) 294, 872
Bsp1286I GDGCHC 2 cut(s) 24, 322
Bsp143I GATC 5 cut(s) 35, 191, 501, 549, 981
Bsp1720I GCTNAGC 1 cut(s) 321
BspACI CCGC 2 cut(s) 289, 291
BspANI GGCC 2 cut(s) 294, 872
BspCNI CTCAG 5 cut(s) 334, 342, 511, 892, 907
BspDI ATCGAT 1 cut(s) 1089
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 3 cut(s) 37, 243, 1003
BspPI GGATC 4 cut(s) 30, 43, 199, 544
BsrI ACTGG 2 cut(s) 368, 1030
BssECI CCNNGG 7 cut(s) 26, 40, 53, 99, 139, 289, 804
BssMI GATC 5 cut(s) 35, 191, 501, 549, 981
BssT1I CCWWGG 4 cut(s) 40, 53, 99, 139
Bst2UI CCWGG 4 cut(s) 28, 189, 554, 805
Bst4CI ACNGT 4 cut(s) 313, 475, 799, 833
Bst6I CTCTTC 1 cut(s) 1004
BstC8I GCNNGC 2 cut(s) 63, 125
BstDEI CTNAG 5 cut(s) 321, 329, 498, 900, 915
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 6 cut(s) 109, 110, 162, 181, 775, 995
BstFNI CGCG 1 cut(s) 291
BstKTI GATC 5 cut(s) 38, 194, 504, 552, 984
BstMAI GTCTC 2 cut(s) 9, 206
BstMBI GATC 5 cut(s) 35, 191, 501, 549, 981
BstMWI GCNNNNNNNGC 2 cut(s) 28, 124
BstNI CCWGG 4 cut(s) 28, 189, 554, 805
BstNSI RCATGY 1 cut(s) 219
BstPAI GACNNNNGTC 1 cut(s) 880
BstSCI CCNGG 5 cut(s) 26, 187, 552, 716, 803
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 52, 186
BstV2I GAAGAC 2 cut(s) 287, 625
BstX2I RGATCY 2 cut(s) 35, 549
BstXI CCANNNNNNTGG 1 cut(s) 560
BstYI RGATCY 2 cut(s) 35, 549
Bsu15I ATCGAT 1 cut(s) 1089
BsuI GTATCC 1 cut(s) 815
BsuRI GGCC 2 cut(s) 294, 872
BsuTUI ATCGAT 1 cut(s) 1089
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 6 cut(s) 109, 110, 162, 181, 775, 995
BtsI GCAGTG 2 cut(s) 129, 888
BtsIMutI CAGTG 5 cut(s) 129, 318, 337, 888, 897
Cac8I GCNNGC 2 cut(s) 63, 125
CaiI CAGNNNCTG 1 cut(s) 1036
Cfr13I GGNCC 1 cut(s) 1001
Cfr42I CCGCGG 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 1089
Csp6I GTAC 3 cut(s) 397, 834, 1147
CviAII CATG 5 cut(s) 32, 216, 544, 771, 1112
CviQI GTAC 3 cut(s) 397, 834, 1147
DdeI CTNAG 5 cut(s) 321, 329, 498, 900, 915
DpnI GATC 5 cut(s) 37, 193, 503, 551, 983
DpnII GATC 5 cut(s) 35, 191, 501, 549, 981
DraIII CACNNNGTG 1 cut(s) 315
DriI GACNNNNNGTC 1 cut(s) 1053
Eam1104I CTCTTC 1 cut(s) 1004
Eam1105I GACNNNNNGTC 1 cut(s) 1053
EarI CTCTTC 1 cut(s) 1004
Eco130I CCWWGG 4 cut(s) 40, 53, 99, 139
Eco24I GRGCYC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 1072
Eco47I GGWCC 1 cut(s) 1001
EcoRII CCWGG 4 cut(s) 26, 187, 552, 803
EcoRV GATATC 1 cut(s) 1072
EcoT14I CCWWGG 4 cut(s) 40, 53, 99, 139
EcoT38I GRGCYC 1 cut(s) 322
ErhI CCWWGG 4 cut(s) 40, 53, 99, 139
FaeI CATG 5 cut(s) 35, 219, 547, 774, 1115
FaqI GGGAC 1 cut(s) 1018
FatI CATG 5 cut(s) 31, 215, 543, 770, 1111
FauI CCCGC 1 cut(s) 296
FbaI TGATCA 1 cut(s) 981
Fnu4HI GCNGC 3 cut(s) 66, 200, 292
FokI GGATG 6 cut(s) 97, 116, 169, 188, 762, 1002
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 3 cut(s) 66, 200, 292
GluI GCNGC 3 cut(s) 66, 200, 292
GsaI CCCAGC 1 cut(s) 156
HaeIII GGCC 2 cut(s) 294, 872
HapII CCGG 2 cut(s) 718, 873
Hin1II CATG 5 cut(s) 35, 219, 547, 774, 1115
HindIII AAGCTT 2 cut(s) 598, 860
HinfI GANTC 3 cut(s) 160, 881, 1158
HpaII CCGG 2 cut(s) 718, 873
HphI GGTGA 4 cut(s) 568, 706, 821, 973
Hpy188I TCNGA 2 cut(s) 501, 981
Hpy188III TCNNGA 5 cut(s) 181, 505, 637, 1064, 1123
HpyAV CCTTC 9 cut(s) 82, 202, 305, 376, 416, 434, 508, 683, 1034
HpyCH4III ACNGT 4 cut(s) 313, 475, 799, 833
HpyCH4V TGCA 5 cut(s) 123, 202, 369, 593, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 124
HpyF3I CTNAG 5 cut(s) 321, 329, 498, 900, 915
Hsp92II CATG 5 cut(s) 35, 219, 547, 774, 1115
Ksp22I TGATCA 1 cut(s) 981
KspI CCGCGG 1 cut(s) 292
Kzo9I GATC 5 cut(s) 35, 191, 501, 549, 981
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 2 cut(s) 52, 186
LweI GCATC 4 cut(s) 334, 616, 784, 980
MaeIII GTNAC 9 cut(s) 71, 143, 226, 667, 697, 712, 882, 895, 1018
MalI GATC 5 cut(s) 37, 193, 503, 551, 983
MboI GATC 5 cut(s) 35, 191, 501, 549, 981
MboII GAAGA 8 cut(s) 41, 292, 391, 442, 520, 541, 625, 1021
MfeI CAATTG 2 cut(s) 570, 933
MflI RGATCY 2 cut(s) 35, 549
MhlI GDGCHC 2 cut(s) 24, 322
MluCI AATT 4 cut(s) 570, 587, 852, 933
MlyI GAGTC 3 cut(s) 154, 890, 1152
MmeI TCCRAC 1 cut(s) 979
MroXI GAANNNNTTC 2 cut(s) 92, 386
MseI TTAA 2 cut(s) 342, 462
MslI CAYNNNNRTG 3 cut(s) 330, 665, 954
MspA1I CMGCKG 1 cut(s) 291
MspI CCGG 2 cut(s) 718, 873
MspR9I CCNGG 5 cut(s) 28, 189, 554, 718, 805
MunI CAATTG 2 cut(s) 570, 933
MvaI CCWGG 4 cut(s) 28, 189, 554, 805
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 2 cut(s) 28, 124
NciI CCSGG 1 cut(s) 718
NdeII GATC 5 cut(s) 35, 191, 501, 549, 981
NlaIII CATG 5 cut(s) 35, 219, 547, 774, 1115
NlaIV GGNNCC 3 cut(s) 37, 243, 1003
NmuCI GTSAC 6 cut(s) 71, 226, 667, 712, 882, 895
NspI RCATGY 1 cut(s) 219
PciI ACATGT 1 cut(s) 215
PdmI GAANNNNTTC 2 cut(s) 92, 386
PflMI CCANNNNNTGG 1 cut(s) 938
PkrI GCNGC 3 cut(s) 67, 201, 293
PleI GAGTC 3 cut(s) 154, 889, 1152
PpsI GAGTC 3 cut(s) 154, 889, 1152
PscI ACATGT 1 cut(s) 215
PshAI GACNNNNGTC 1 cut(s) 880
Psp6I CCWGG 4 cut(s) 26, 187, 552, 803
PspFI CCCAGC 1 cut(s) 152
PspGI CCWGG 4 cut(s) 26, 187, 552, 803
PspN4I GGNNCC 3 cut(s) 37, 243, 1003
PspPI GGNCC 1 cut(s) 1001
PstNI CAGNNNCTG 1 cut(s) 1036
PsuI RGATCY 2 cut(s) 35, 549
RsaI GTAC 3 cut(s) 398, 835, 1148
RsaNI GTAC 3 cut(s) 397, 834, 1147
RseI CAYNNNNRTG 3 cut(s) 330, 665, 954
SacII CCGCGG 1 cut(s) 292
SaqAI TTAA 2 cut(s) 342, 462
SatI GCNGC 3 cut(s) 66, 200, 292
Sau3AI GATC 5 cut(s) 35, 191, 501, 549, 981
Sau96I GGNCC 1 cut(s) 1001
ScaI AGTACT 2 cut(s) 398, 1148
SchI GAGTC 3 cut(s) 154, 890, 1152
ScrFI CCNGG 5 cut(s) 28, 189, 554, 718, 805
SduI GDGCHC 2 cut(s) 24, 322
SfaNI GCATC 4 cut(s) 334, 616, 784, 980
Sfr303I CCGCGG 1 cut(s) 292
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 1 cut(s) 1001
SmiMI CAYNNNNRTG 3 cut(s) 330, 665, 954
SmlI CTYRAG 4 cut(s) 1040, 1064, 1079, 1160
SmoI CTYRAG 4 cut(s) 1040, 1064, 1079, 1160
Sse9I AATT 4 cut(s) 570, 587, 852, 933
SsiI CCGC 2 cut(s) 289, 291
SspI AATATT 1 cut(s) 436
StyD4I CCNGG 5 cut(s) 26, 187, 552, 716, 803
StyI CCWWGG 4 cut(s) 40, 53, 99, 139
TaaI ACNGT 4 cut(s) 313, 475, 799, 833
TaqI TCGA 3 cut(s) 180, 481, 1089
TasI AATT 4 cut(s) 570, 587, 852, 933
TatI WGTACW 2 cut(s) 396, 1146
TauI GCSGC 1 cut(s) 294
Tru1I TTAA 2 cut(s) 342, 462
Tru9I TTAA 2 cut(s) 342, 462
TscAI CASTG 5 cut(s) 136, 318, 337, 895, 904
TseFI GTSAC 6 cut(s) 71, 226, 667, 712, 882, 895
TseI GCWGC 2 cut(s) 65, 199
Tsp45I GTSAC 6 cut(s) 71, 226, 667, 712, 882, 895
TspDTI ATGAA 7 cut(s) 426, 527, 660, 702, 780, 857, 1075
TspRI CASTG 5 cut(s) 136, 318, 337, 895, 904
Van91I CCANNNNNTGG 1 cut(s) 938
VpaK11BI GGWCC 1 cut(s) 1001
XapI RAATTY 2 cut(s) 587, 852
XceI RCATGY 1 cut(s) 219
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmnI GAANNNNTTC 2 cut(s) 92, 386
ZrmI AGTACT 2 cut(s) 398, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.