Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0006620

Physical Location & Seq

Genomic Coordinates Reverse (-)

18225294 .. 18228876

Gene Structure 3583 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g22150.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1515 bp

ATGGGTGAGAAGCTTTGGGTGAATGAAGAAGAAAAGGCCGTAGTGGAGTCAGTACTGGGTACAGAAGCATGCCAGTTGTTGATCTCATTGGCTTCGGAGAACTTGTTGTCCGACTTGGCTAGGCCTCCAGGACAATTGGGTGTGCAGCAGGGGCTGTCCCAGCTTGTCCAAGGGTCCAGTTGGAATTATGCTGTCTTCTGGCAGGTTGTTGGATTGAAATCTGGTGGAGCCGCCTTGATTTGGGGCGATGGACATTGTAAAGATACCAAGGGTGGTGGAGTTGGAGACGAGAGCTCTACTAGGGATGGCAACTTGGAGGGAGTGCAGAAGAAGAATGAAGAGGTGAGGAAGTGGGTGCTTGGGAAGCTCCACTCATGTTTCGGTGGTTCGGGTGAGGATAACTATGCAAGGAGGTTGGATAGGTTGTCTGATGTGGAAATGTTTTACCTGACTTCAATGTACTTTAAATTTCAACTTGATTCACCGTATGGTCCTGGGGAGTCGTACAAGTCTGGTAAATCAATTTGGGTTTCGAACGTTGCTAGTTGCTTACATCATTACCAGTCGAGATCGTTTTTAGCTAGATTGGCAGGGTTCCAAACAGTTGTGTTTGTACCCATGAAATCAGGAGTTGTGGAGCTTGGTTCAGTCAAATCAATTCTGGAAGAACAGAATGTTGTGGATATGGTCAGAAGTGTATTTGGGGTTCCTAGCTCTGTCCAGGCAAAGATATATCCAAAGATTTTCGGGCATGAGCTTAGTCTCGGTGGTCCAAAACCACAACCGATAAACATTGCCTTCTGCCCAAAGGTTGAAACAGACTCTTCATTTCCTTCAGAATCTTTTGACTTACAAGCAATAGGCACTTCAAATGGATGTCGCACTGAGGATAGTGAAGTTAAACTGTTTCCACACTTGAGCCAAATTATGGGTGGGAATTTCAGTTCTACAGCTGGTGTTTCAAGTTCAGAGCTGCCTAAAGACGAGTCATCAGCACAAATTGATGAAAGGAAACCTAGAAAGAGAGGGAGAAAGCCTGCCAATGGAAGAGAAGAACCCTTGAATCATGTGGAAGCAGAGCGGCAGAGGCGTGAGAAGCTTAACCAGCGGTTCTACGCACTTAGAGCTGTTGTTCCCAACATATCAAAGATGGATAAAGCCTCTCTCCTTGGTGATGCCATTACCTATATCAATGATCTACAGACGAAGATCAGGGTCATGGAAACTGAGAAGCAGATGGGAAGCAGCAGCCACATGCAGTTCCCGATTCCAGAGATTGATTTCCAGGAACGACAAGAGGATGCTGTTGTGACGATGAACTGCCCACTGGGTTCTCACCCTGTTTCTGATGTCATCAGAACACTTAGGGAACATAGCATTGTAGCTCAAGATACTAATGTGTCCGTAACAGAGGATGACACTGTTATTCATACATTCTCCATTCCAACCCAAGGTGGTGATGCCGAGCAGTTAAAGGAGAAGCTGGTGGCTGCTCTTTCAAAATCATCCGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009892 GO:0010468 GO:0010556 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048519 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

505

Amino Acids

55.38

Weight (kDa)

5.77

Isoelectric Point (pI)

47.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	49 - 234	2.7e-47	bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH	PF00010	355 - 401	2.5e-12	Helix-loop-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006620)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16430
fragaria_vesca	FvH4_2g22150 FvH4_2g22150
malus_domestica	MD05G1202500.v1.1 MD05G1202900.v1.1 MD10G1190000.v1.1
prunus_persica	Prupe.8G228700_v2.0.a1 Prupe.8G228700_v2.0.a1 Prupe.8G228700_v2.0.a1 Prupe.8G228700_v2.0.a1
pyrus_communis	pycom05g18900 pycom05g18950 pycom10g16360 pycom10g16370
rosa_chinensis	RchiOBHm_Chr6g0288981
rosa_laevigata	RLG00000012333 RLG00000013932
rosa_multiflora	Rmu_sc0000666.1_g000010
rosa_roxburghii	Rroxscaffold_7G00179620
rosa_rugosa	Rorug06G0199900
rosa_samantha	Rh6AG311600 Rh6BG318100 Rh6CG325000 Rh6DG310800
rosa_wichuraiana	Rw6G013790 Rw6G026880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	193
AccB7I	CCANNNNNTGG	1 cut(s)	928
AccBSI	CCGCTC	1 cut(s)	1081
AciI	CCGC	3 cut(s)	231, 1081, 1108
AclI	AACGTT	1 cut(s)	537
AcsI	RAATTY	2 cut(s)	467, 937
AcuI	CTGAAG	1 cut(s)	819
AfaI	GTAC	5 cut(s)	54, 61, 461, 506, 615
AfiI	CCNNNNNNNGG	4 cut(s)	240, 928, 1043, 1451
AgsI	TTSAA	8 cut(s)	217, 456, 473, 815, 870, 963, 1063, 1500
AjnI	CCWGG	4 cut(s)	127, 493, 720, 1284
AloI	GAACNNNNNNTCC	4 cut(s)	92, 124, 928, 960
Alw21I	GWGCWC	1 cut(s)	296
Alw26I	GTCTC	2 cut(s)	279, 767
AlwNI	CAGNNNCTG	1 cut(s)	154
AoxI	GGCC	2 cut(s)	36, 122
ApeKI	GCWGC	5 cut(s)	145, 973, 1245, 1248, 1490
ApoI	RAATTY	2 cut(s)	467, 937
AspS9I	GGNCC	3 cut(s)	174, 491, 770
AsuHPI	GGTGA	8 cut(s)	17, 31, 355, 404, 474, 1184, 1328, 1469
AsuII	TTCGAA	1 cut(s)	533
AvaII	GGWCC	3 cut(s)	174, 491, 770
BaeI	ACNNNNGTAYC	2 cut(s)	1383, 1416
BanII	GRGCYC	1 cut(s)	296
BbsI	GAAGAC	1 cut(s)	187
Bbv12I	GWGCWC	1 cut(s)	296
BbvI	GCAGC	5 cut(s)	157, 960, 1257, 1260, 1477
BccI	CCATC	4 cut(s)	242, 299, 1144, 1231
BceAI	ACGGC	1 cut(s)	23
BciT130I	CCWGG	4 cut(s)	129, 495, 722, 1286
BcoDI	GTCTC	2 cut(s)	279, 767
BfaI	CTAG	6 cut(s)	120, 300, 543, 582, 711, 1017
BfmI	CTRYAG	2 cut(s)	948, 1199
BfuAI	ACCTGC	1 cut(s)	193
BisI	GCNGC	7 cut(s)	146, 231, 974, 1082, 1246, 1249, 1491
BlsI	GCNGC	7 cut(s)	147, 232, 975, 1083, 1247, 1250, 1492
BmcAI	AGTACT	1 cut(s)	54
Bme1390I	CCNGG	4 cut(s)	129, 495, 722, 1286
Bme18I	GGWCC	3 cut(s)	174, 491, 770
BmgT120I	GGNCC	3 cut(s)	174, 491, 770
BmiI	GGNNCC	4 cut(s)	175, 229, 596, 708
BmrFI	CCNGG	4 cut(s)	129, 495, 722, 1286
BmrI	ACTGGG	2 cut(s)	65, 1337
BmsI	GCATC	3 cut(s)	1165, 1291, 1450
BmuI	ACTGGG	2 cut(s)	65, 1337
BpiI	GAAGAC	1 cut(s)	187
BpmI	CTGGAG	1 cut(s)	111
Bpu14I	TTCGAA	1 cut(s)	533
BpuEI	CTTGAG	2 cut(s)	937, 1371
BsaBI	GATNNNNATC	1 cut(s)	217
BsaJI	CCNNGG	5 cut(s)	169, 267, 494, 1168, 1450
BsaXI	ACNNNNNCTCC	2 cut(s)	89, 119
Bsc4I	CCNNNNNNNGG	4 cut(s)	240, 928, 1043, 1451
Bse1I	ACTGG	5 cut(s)	60, 73, 177, 562, 1332
Bse3DI	GCAATG	1 cut(s)	792
Bse8I	GATNNNNATC	1 cut(s)	217
BseBI	CCWGG	4 cut(s)	129, 495, 722, 1286
BseDI	CCNNGG	5 cut(s)	169, 267, 494, 1168, 1450
BseGI	GGATG	5 cut(s)	310, 881, 1306, 1420, 1505
BseJI	GATNNNNATC	1 cut(s)	217
BseLI	CCNNNNNNNGG	4 cut(s)	240, 928, 1043, 1451
BseMI	GCAATG	1 cut(s)	792
BseMII	CTCAG	2 cut(s)	876, 1218
BseNI	ACTGG	5 cut(s)	60, 73, 177, 562, 1332
BseXI	GCAGC	5 cut(s)	157, 960, 1257, 1260, 1477
BseYI	CCCAGC	1 cut(s)	159
BsgI	GTGCAG	2 cut(s)	164, 344
BshFI	GGCC	2 cut(s)	38, 124
BsiHKAI	GWGCWC	1 cut(s)	296
BslFI	GGGAC	1 cut(s)	142
BslI	CCNNNNNNNGG	4 cut(s)	240, 928, 1043, 1451
BsmAI	GTCTC	2 cut(s)	279, 767
BsmBI	CGTCTC	1 cut(s)	279
BsmFI	GGGAC	1 cut(s)	142
BsnI	GGCC	2 cut(s)	38, 124
Bsp119I	TTCGAA	1 cut(s)	533
Bsp1286I	GDGCHC	1 cut(s)	296
Bsp143I	GATC	4 cut(s)	81, 569, 1195, 1209
BspACI	CCGC	3 cut(s)	231, 1081, 1108
BspANI	GGCC	2 cut(s)	38, 124
BspCNI	CTCAG	2 cut(s)	877, 1219
BspLI	GGNNCC	4 cut(s)	175, 229, 596, 708
BspMI	ACCTGC	1 cut(s)	193
BspT104I	TTCGAA	1 cut(s)	533
BsrBI	CCGCTC	1 cut(s)	1081
BsrDI	GCAATG	1 cut(s)	792
BsrI	ACTGG	5 cut(s)	60, 73, 177, 562, 1332
BssECI	CCNNGG	5 cut(s)	169, 267, 494, 1168, 1450
BssMI	GATC	4 cut(s)	81, 569, 1195, 1209
BssT1I	CCWWGG	4 cut(s)	169, 267, 1168, 1450
Bst2UI	CCWGG	4 cut(s)	129, 495, 722, 1286
Bst4CI	ACNGT	4 cut(s)	486, 604, 906, 1423
Bst6I	CTCTTC	3 cut(s)	333, 829, 1042
BstBI	TTCGAA	1 cut(s)	533
BstC8I	GCNNGC	2 cut(s)	70, 1038
BstDEI	CTNAG	5 cut(s)	758, 885, 1121, 1227, 1364
BstF5I	GGATG	5 cut(s)	310, 881, 1306, 1420, 1505
BstKTI	GATC	4 cut(s)	84, 572, 1198, 1212
BstMAI	GTCTC	2 cut(s)	279, 767
BstMBI	GATC	4 cut(s)	81, 569, 1195, 1209
BstMWI	GCNNNNNNNGC	8 cut(s)	151, 160, 364, 587, 1087, 1096, 1105, 1124
BstNI	CCWGG	4 cut(s)	129, 495, 722, 1286
BstNSI	RCATGY	2 cut(s)	72, 1258
BstSCI	CCNGG	4 cut(s)	127, 493, 720, 1284
BstSFI	CTRYAG	2 cut(s)	948, 1199
BstV1I	GCAGC	5 cut(s)	157, 960, 1257, 1260, 1477
BstV2I	GAAGAC	1 cut(s)	187
BsuRI	GGCC	2 cut(s)	38, 124
BtgZI	GCGATG	1 cut(s)	261
BtsCI	GGATG	5 cut(s)	310, 881, 1306, 1420, 1505
BtsIMutI	CAGTG	3 cut(s)	882, 1325, 1419
BveI	ACCTGC	1 cut(s)	193
Cac8I	GCNNGC	2 cut(s)	70, 1038
CaiI	CAGNNNCTG	1 cut(s)	154
Cfr13I	GGNCC	3 cut(s)	174, 491, 770
Csp6I	GTAC	5 cut(s)	53, 60, 460, 505, 614
CspCI	CAANNNNNGTGG	2 cut(s)	256, 291
CviAII	CATG	7 cut(s)	69, 375, 619, 752, 1067, 1219, 1255
CviQI	GTAC	5 cut(s)	53, 60, 460, 505, 614
DdeI	CTNAG	5 cut(s)	758, 885, 1121, 1227, 1364
DpnI	GATC	4 cut(s)	83, 571, 1197, 1211
DpnII	GATC	4 cut(s)	81, 569, 1195, 1209
DraI	TTTAAA	1 cut(s)	466
Eam1104I	CTCTTC	3 cut(s)	333, 829, 1042
EarI	CTCTTC	3 cut(s)	333, 829, 1042
Ecl136II	GAGCTC	1 cut(s)	294
Eco130I	CCWWGG	4 cut(s)	169, 267, 1168, 1450
Eco147I	AGGCCT	1 cut(s)	124
Eco24I	GRGCYC	1 cut(s)	296
Eco47I	GGWCC	3 cut(s)	174, 491, 770
Eco53kI	GAGCTC	1 cut(s)	294
Eco57I	CTGAAG	1 cut(s)	819
EcoICRI	GAGCTC	1 cut(s)	294
EcoRII	CCWGG	4 cut(s)	127, 493, 720, 1284
EcoT14I	CCWWGG	4 cut(s)	169, 267, 1168, 1450
EcoT38I	GRGCYC	1 cut(s)	296
ErhI	CCWWGG	4 cut(s)	169, 267, 1168, 1450
Esp3I	CGTCTC	1 cut(s)	279
FaeI	CATG	7 cut(s)	72, 378, 622, 755, 1070, 1222, 1258
FaqI	GGGAC	1 cut(s)	142
FatI	CATG	7 cut(s)	68, 374, 618, 751, 1066, 1218, 1254
Fnu4HI	GCNGC	7 cut(s)	146, 231, 974, 1082, 1246, 1249, 1491
FokI	GGATG	5 cut(s)	317, 888, 1313, 1427, 1492
FriOI	GRGCYC	1 cut(s)	296
Fsp4HI	GCNGC	7 cut(s)	146, 231, 974, 1082, 1246, 1249, 1491
FspBI	CTAG	6 cut(s)	120, 300, 543, 582, 711, 1017
GluI	GCNGC	7 cut(s)	146, 231, 974, 1082, 1246, 1249, 1491
GsaI	CCCAGC	1 cut(s)	163
GsuI	CTGGAG	1 cut(s)	111
HaeIII	GGCC	2 cut(s)	38, 124
Hin1II	CATG	7 cut(s)	72, 378, 622, 755, 1070, 1222, 1258
HindIII	AAGCTT	2 cut(s)	11, 1097
HinfI	GANTC	8 cut(s)	47, 479, 500, 821, 839, 986, 1063, 1267
HphI	GGTGA	8 cut(s)	17, 31, 355, 404, 474, 1184, 1328, 1469
Hpy188I	TCNGA	8 cut(s)	97, 112, 430, 692, 838, 970, 1348, 1358
Hpy188III	TCNNGA	6 cut(s)	567, 627, 662, 1264, 1271, 1388
HpyAV	CCTTC	2 cut(s)	808, 843
HpyCH4III	ACNGT	4 cut(s)	486, 604, 906, 1423
HpyCH4IV	ACGT	1 cut(s)	537
HpyCH4V	TGCA	4 cut(s)	145, 325, 407, 1258
HpyF10VI	GCNNNNNNNGC	8 cut(s)	151, 160, 364, 587, 1087, 1096, 1105, 1124
HpyF3I	CTNAG	5 cut(s)	758, 885, 1121, 1227, 1364
HpySE526I	ACGT	1 cut(s)	537
Hsp92II	CATG	7 cut(s)	72, 378, 622, 755, 1070, 1222, 1258
Kzo9I	GATC	4 cut(s)	81, 569, 1195, 1209
LmnI	GCTCC	3 cut(s)	227, 372, 637
Lsp1109I	GCAGC	5 cut(s)	157, 960, 1257, 1260, 1477
LweI	GCATC	3 cut(s)	1165, 1291, 1450
MaeI	CTAG	6 cut(s)	120, 300, 543, 582, 711, 1017
MaeII	ACGT	1 cut(s)	537
MaeIII	GTNAC	2 cut(s)	1309, 1405
MalI	GATC	4 cut(s)	83, 571, 1197, 1211
MbiI	CCGCTC	1 cut(s)	1081
MboI	GATC	4 cut(s)	81, 569, 1195, 1209
MfeI	CAATTG	1 cut(s)	134
MhlI	GDGCHC	1 cut(s)	296
MluCI	AATT	8 cut(s)	134, 184, 467, 522, 657, 924, 937, 999
MlyI	GAGTC	4 cut(s)	56, 509, 815, 995
MmeI	TCCRAC	6 cut(s)	135, 161, 190, 262, 396, 1469
MseI	TTAA	4 cut(s)	465, 900, 1101, 1472
MspA1I	CMGCKG	2 cut(s)	953, 1108
MspR9I	CCNGG	4 cut(s)	129, 495, 722, 1286
MunI	CAATTG	1 cut(s)	134
MvaI	CCWGG	4 cut(s)	129, 495, 722, 1286
MwoI	GCNNNNNNNGC	8 cut(s)	151, 160, 364, 587, 1087, 1096, 1105, 1124
NdeII	GATC	4 cut(s)	81, 569, 1195, 1209
NlaIII	CATG	7 cut(s)	72, 378, 622, 755, 1070, 1222, 1258
NlaIV	GGNNCC	4 cut(s)	175, 229, 596, 708
NmeAIII	GCCGAG	1 cut(s)	1489
NmuCI	GTSAC	1 cut(s)	1309
NspI	RCATGY	2 cut(s)	72, 1258
NspV	TTCGAA	1 cut(s)	533
PaeI	GCATGC	1 cut(s)	72
PceI	AGGCCT	1 cut(s)	124
PfeI	GAWTC	4 cut(s)	479, 839, 1063, 1267
PflMI	CCANNNNNTGG	1 cut(s)	928
PfoI	TCCNGGA	2 cut(s)	127, 1284
PkrI	GCNGC	7 cut(s)	147, 232, 975, 1083, 1247, 1250, 1492
PleI	GAGTC	4 cut(s)	55, 508, 815, 994
PpsI	GAGTC	4 cut(s)	55, 508, 815, 994
Psp124BI	GAGCTC	1 cut(s)	296
Psp1406I	AACGTT	1 cut(s)	537
Psp6I	CCWGG	4 cut(s)	127, 493, 720, 1284
PspFI	CCCAGC	1 cut(s)	159
PspGI	CCWGG	4 cut(s)	127, 493, 720, 1284
PspN4I	GGNNCC	4 cut(s)	175, 229, 596, 708
PspPI	GGNCC	3 cut(s)	174, 491, 770
PsrI	GAACNNNNNNTAC	2 cut(s)	690, 722
PstNI	CAGNNNCTG	1 cut(s)	154
PvuII	CAGCTG	1 cut(s)	953
RsaI	GTAC	5 cut(s)	54, 61, 461, 506, 615
RsaNI	GTAC	5 cut(s)	53, 60, 460, 505, 614
SacI	GAGCTC	1 cut(s)	296
SaqAI	TTAA	4 cut(s)	465, 900, 1101, 1472
SatI	GCNGC	7 cut(s)	146, 231, 974, 1082, 1246, 1249, 1491
Sau3AI	GATC	4 cut(s)	81, 569, 1195, 1209
Sau96I	GGNCC	3 cut(s)	174, 491, 770
ScaI	AGTACT	1 cut(s)	54
SchI	GAGTC	4 cut(s)	56, 509, 815, 995
ScrFI	CCNGG	4 cut(s)	129, 495, 722, 1286
SduI	GDGCHC	1 cut(s)	296
SfaNI	GCATC	3 cut(s)	1165, 1291, 1450
SfcI	CTRYAG	2 cut(s)	948, 1199
SfuI	TTCGAA	1 cut(s)	533
SinI	GGWCC	3 cut(s)	174, 491, 770
SmlI	CTYRAG	2 cut(s)	916, 1386
SmoI	CTYRAG	2 cut(s)	916, 1386
SphI	GCATGC	1 cut(s)	72
Sse9I	AATT	8 cut(s)	134, 184, 467, 522, 657, 924, 937, 999
SseBI	AGGCCT	1 cut(s)	124
SsiI	CCGC	3 cut(s)	231, 1081, 1108
SspMI	CTAG	6 cut(s)	120, 300, 543, 582, 711, 1017
SstI	GAGCTC	1 cut(s)	296
StuI	AGGCCT	1 cut(s)	124
StyD4I	CCNGG	4 cut(s)	127, 493, 720, 1284
StyI	CCWWGG	4 cut(s)	169, 267, 1168, 1450
TaaI	ACNGT	4 cut(s)	486, 604, 906, 1423
TaiI	ACGT	1 cut(s)	540
TaqI	TCGA	2 cut(s)	533, 566
TasI	AATT	8 cut(s)	134, 184, 467, 522, 657, 924, 937, 999
TatI	WGTACW	2 cut(s)	52, 459
TauI	GCSGC	2 cut(s)	233, 1084
TfiI	GAWTC	4 cut(s)	479, 839, 1063, 1267
Tru1I	TTAA	4 cut(s)	465, 900, 1101, 1472
Tru9I	TTAA	4 cut(s)	465, 900, 1101, 1472
TscAI	CASTG	3 cut(s)	889, 1332, 1426
TseFI	GTSAC	1 cut(s)	1309
TseI	GCWGC	5 cut(s)	145, 973, 1245, 1248, 1490
Tsp45I	GTSAC	1 cut(s)	1309
TspDTI	ATGAA	7 cut(s)	39, 351, 635, 816, 1020, 1331, 1418
TspGWI	ACGGA	2 cut(s)	1393, 1498
TspRI	CASTG	3 cut(s)	889, 1332, 1426
Van91I	CCANNNNNTGG	1 cut(s)	928
VpaK11BI	GGWCC	3 cut(s)	174, 491, 770
XapI	RAATTY	2 cut(s)	467, 937
XceI	RCATGY	2 cut(s)	72, 1258
XcmI	CCANNNNNNNNNTGG	1 cut(s)	929
XspI	CTAG	6 cut(s)	120, 300, 543, 582, 711, 1017
ZrmI	AGTACT	1 cut(s)	54

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.