RLG00000013932
BHLH Family

Transcription factor bHLH3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006620
Physical Location & Seq
Forward (+)
44458275 .. 44459114
840 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013932

Sequence Viewer

Length: 663 bp
ATGGGTGAGAAGCTTGGGGTGAATGAGGAAGAAAAGGTCATAGTGGAGTCAGTACTAGGCACCGAAGCGTGCCAGGTGTTGATCTCATTGGCTTCGGAGAACTTGTTGTCCGACTTGGCTAGGCCTCCGGGACAATTGGGAGTGCAGCAGGGGCTGTCCCAGCTTGTCCAAGGGTCCACTGGTCCAAGACACCAAGGGTGGTGGGTTGGAGACGAGAGTTCTAGTAGACATAGCAACTTAGAAGAAGAATGTGGAGGTGAGTGGGTGCTTGGGAAGCTCCACTCATGTTTTGGTGGTTTGGGTGAGGATAACTATGCGAGGAGGTTGGATCGGTTGTCAGATGTGGAAATGTTTTACCTGACTTCAATGTACTTTAAATTTCAACTTGATTCACCGTATGGTCCTAGGGAGTCGTACAAGTCTTGTAAATCAATTTGGGCTTCAAATGTTGCTAGTTGCTTACATCATTACCAGTCGAGGTCATTTTTAGCTAGATTGGCAGGGTTCCAAACAGTGGTGTTTGTACCTATGAAATCAGGAGTTGTGGAGCTTGGTTCAGTCAAATCAATTCCGGAAGAACAGAATATTGTGGATGTGGTCAGAAGTGTATTTGGGGAATCTAGCTCTGTTCAAGCAAAGAATCTTTTGACTTACAAGCAATAG

Protein Analysis

221

Amino Acids

24.3

Weight (kDa)

5.42

Isoelectric Point (pI)

56.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 82 - 204 1.3e-26 bHLH-MYC and R2R3-MYB transcription factors N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 514
AccI GTMKAC 1 cut(s) 226
AccIII TCCGGA 1 cut(s) 571
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 1 cut(s) 377
AfaI GTAC 4 cut(s) 54, 371, 416, 525
AfiI CCNNNNNNNGG 1 cut(s) 514
AgsI TTSAA 4 cut(s) 366, 383, 444, 632
AjnI CCWGG 1 cut(s) 72
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 6 cut(s) 13, 163, 277, 491, 550, 624
AluI AGCT 6 cut(s) 13, 163, 277, 491, 550, 624
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 1 cut(s) 336
AlwNI CAGNNNCTG 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 1 cut(s) 122
ApeKI GCWGC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 377
AspA2I CCTAGG 1 cut(s) 404
AspS9I GGNCC 3 cut(s) 174, 182, 401
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 5 cut(s) 17, 31, 269, 314, 384
AvaII GGWCC 3 cut(s) 174, 182, 401
AvrII CCTAGG 1 cut(s) 404
BanI GGYRCC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 157
BciT130I CCWGG 1 cut(s) 74
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 7 cut(s) 56, 120, 222, 405, 453, 492, 621
BisI GCNGC 1 cut(s) 146
BlnI CCTAGG 1 cut(s) 404
BlsI GCNGC 1 cut(s) 147
BmcAI AGTACT 1 cut(s) 54
Bme1390I CCNGG 2 cut(s) 74, 129
Bme18I GGWCC 3 cut(s) 174, 182, 401
BmgT120I GGNCC 3 cut(s) 174, 182, 401
BmiI GGNNCC 3 cut(s) 61, 175, 506
BmrFI CCNGG 2 cut(s) 74, 129
BpuMI CCSGG 1 cut(s) 129
BsaJI CCNNGG 3 cut(s) 169, 193, 404
BsaWI WCCGGW 1 cut(s) 571
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse1I ACTGG 2 cut(s) 184, 472
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 3 cut(s) 169, 193, 404
BseGI GGATG 1 cut(s) 598
BseLI CCNNNNNNNGG 1 cut(s) 514
BseNI ACTGG 2 cut(s) 184, 472
BseRI GAGGAG 1 cut(s) 334
BseXI GCAGC 1 cut(s) 157
BseYI CCCAGC 1 cut(s) 159
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 1 cut(s) 124
BshNI GGYRCC 1 cut(s) 59
BsiSI CCGG 2 cut(s) 128, 572
BslFI GGGAC 2 cut(s) 142, 144
BslI CCNNNNNNNGG 1 cut(s) 514
BsmAI GTCTC 1 cut(s) 204
BsmBI CGTCTC 1 cut(s) 204
BsmFI GGGAC 2 cut(s) 142, 144
BsnI GGCC 1 cut(s) 124
Bsp13I TCCGGA 1 cut(s) 571
Bsp143I GATC 2 cut(s) 81, 328
BspANI GGCC 1 cut(s) 124
BspEI TCCGGA 1 cut(s) 571
BspLI GGNNCC 3 cut(s) 61, 175, 506
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 59
BsrI ACTGG 2 cut(s) 184, 472
BssECI CCNNGG 3 cut(s) 169, 193, 404
BssMI GATC 2 cut(s) 81, 328
BssT1I CCWWGG 3 cut(s) 169, 193, 404
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 2 cut(s) 396, 514
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 598
BstKTI GATC 2 cut(s) 84, 331
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 2 cut(s) 81, 328
BstMWI GCNNNNNNNGC 4 cut(s) 151, 160, 274, 497
BstNI CCWGG 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 72, 127
BstV1I GCAGC 1 cut(s) 157
BsuRI GGCC 1 cut(s) 124
BtsCI GGATG 1 cut(s) 598
BtsIMutI CAGTG 2 cut(s) 177, 519
Cac8I GCNNGC 1 cut(s) 70
CaiI CAGNNNCTG 1 cut(s) 154
Cfr13I GGNCC 3 cut(s) 174, 182, 401
Csp6I GTAC 4 cut(s) 53, 370, 415, 524
CspCI CAANNNNNGTGG 2 cut(s) 182, 217
CviAII CATG 1 cut(s) 285
CviQI GTAC 4 cut(s) 53, 370, 415, 524
DdeI CTNAG 1 cut(s) 238
DpnI GATC 2 cut(s) 83, 330
DpnII GATC 2 cut(s) 81, 328
DraI TTTAAA 1 cut(s) 376
Eco130I CCWWGG 3 cut(s) 169, 193, 404
Eco147I AGGCCT 1 cut(s) 124
Eco47I GGWCC 3 cut(s) 174, 182, 401
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 3 cut(s) 169, 193, 404
ErhI CCWWGG 3 cut(s) 169, 193, 404
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 1 cut(s) 288
FaiI YATR 6 cut(s) 41, 231, 286, 315, 399, 530
FaqI GGGAC 2 cut(s) 142, 144
FatI CATG 1 cut(s) 284
FblI GTMKAC 1 cut(s) 226
Fnu4HI GCNGC 1 cut(s) 146
FokI GGATG 1 cut(s) 605
Fsp4HI GCNGC 1 cut(s) 146
FspBI CTAG 7 cut(s) 56, 120, 222, 405, 453, 492, 621
GluI GCNGC 1 cut(s) 146
GsaI CCCAGC 1 cut(s) 163
HaeIII GGCC 1 cut(s) 124
HapII CCGG 2 cut(s) 128, 572
Hin1II CATG 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 5 cut(s) 47, 389, 410, 617, 640
HpaII CCGG 2 cut(s) 128, 572
HphI GGTGA 5 cut(s) 17, 31, 269, 314, 384
Hpy166II GTNNAC 2 cut(s) 177, 227
Hpy188I TCNGA 4 cut(s) 97, 112, 340, 602
Hpy188III TCNNGA 2 cut(s) 537, 572
Hpy8I GTNNAC 2 cut(s) 177, 227
HpyCH4III ACNGT 2 cut(s) 396, 514
HpyCH4V TGCA 1 cut(s) 145
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 160, 274, 497
HpyF3I CTNAG 1 cut(s) 238
Hsp92II CATG 1 cut(s) 288
Kpn2I TCCGGA 1 cut(s) 571
Kzo9I GATC 2 cut(s) 81, 328
LmnI GCTCC 2 cut(s) 282, 547
Lsp1109I GCAGC 1 cut(s) 157
MaeI CTAG 7 cut(s) 56, 120, 222, 405, 453, 492, 621
MalI GATC 2 cut(s) 83, 330
MboI GATC 2 cut(s) 81, 328
MboII GAAGA 4 cut(s) 41, 254, 257, 587
MfeI CAATTG 1 cut(s) 134
MluCI AATT 4 cut(s) 134, 377, 432, 567
MlyI GAGTC 2 cut(s) 56, 419
MmeI TCCRAC 3 cut(s) 135, 187, 306
MnlI CCTC 7 cut(s) 19, 135, 248, 298, 312, 315, 471
MroI TCCGGA 1 cut(s) 571
MseI TTAA 1 cut(s) 375
MspI CCGG 2 cut(s) 128, 572
MspR9I CCNGG 2 cut(s) 74, 129
MunI CAATTG 1 cut(s) 134
MvaI CCWGG 1 cut(s) 74
MwoI GCNNNNNNNGC 4 cut(s) 151, 160, 274, 497
NciI CCSGG 1 cut(s) 129
NdeII GATC 2 cut(s) 81, 328
NlaIII CATG 1 cut(s) 288
NlaIV GGNNCC 3 cut(s) 61, 175, 506
PceI AGGCCT 1 cut(s) 124
PfeI GAWTC 3 cut(s) 389, 617, 640
PflMI CCANNNNNTGG 1 cut(s) 514
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 1 cut(s) 147
PleI GAGTC 2 cut(s) 55, 418
PpsI GAGTC 2 cut(s) 55, 418
Psp6I CCWGG 1 cut(s) 72
PspFI CCCAGC 1 cut(s) 159
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 3 cut(s) 61, 175, 506
PspPI GGNCC 3 cut(s) 174, 182, 401
PstNI CAGNNNCTG 1 cut(s) 154
RsaI GTAC 4 cut(s) 54, 371, 416, 525
RsaNI GTAC 4 cut(s) 53, 370, 415, 524
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 146
Sau3AI GATC 2 cut(s) 81, 328
Sau96I GGNCC 3 cut(s) 174, 182, 401
ScaI AGTACT 1 cut(s) 54
SchI GAGTC 2 cut(s) 56, 419
ScrFI CCNGG 2 cut(s) 74, 129
SinI GGWCC 3 cut(s) 174, 182, 401
Sse9I AATT 4 cut(s) 134, 377, 432, 567
SseBI AGGCCT 1 cut(s) 124
SspI AATATT 1 cut(s) 586
SspMI CTAG 7 cut(s) 56, 120, 222, 405, 453, 492, 621
StuI AGGCCT 1 cut(s) 124
StyD4I CCNGG 2 cut(s) 72, 127
StyI CCWWGG 3 cut(s) 169, 193, 404
TaaI ACNGT 2 cut(s) 396, 514
TaqI TCGA 1 cut(s) 476
TasI AATT 4 cut(s) 134, 377, 432, 567
TatI WGTACW 2 cut(s) 52, 369
TfiI GAWTC 3 cut(s) 389, 617, 640
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 184, 519
TseI GCWGC 1 cut(s) 145
TspDTI ATGAA 1 cut(s) 545
TspRI CASTG 2 cut(s) 184, 519
Van91I CCANNNNNTGG 1 cut(s) 514
VpaK11BI GGWCC 3 cut(s) 174, 182, 401
XapI RAATTY 1 cut(s) 377
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 287
XmaJI CCTAGG 1 cut(s) 404
XmiI GTMKAC 1 cut(s) 226
XspI CTAG 7 cut(s) 56, 120, 222, 405, 453, 492, 621
ZrmI AGTACT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.