Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0006620

Physical Location & Seq

Genomic Coordinates Reverse (-)

21888207 .. 21889715

Gene Structure 1509 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g18950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGGTGAGAAATTTTGGCTGAATGAAGAAGACAAGGTCGTGATGGAGTCAGTACTGGGTACCGAAGCTTGCCAGTTCTTGATCTCACTGGCTTCTGAAAATGTTTTGCCAGAGTTGATTAGGCCGCCGGGTAAATTGGGTGTGCAGCAGGGTCTGTGCCAGCTTGTCAATGGGTCGAATTGGAATTATGCCATTTTCTGGCAGGCTGTTAGCAAGAAATCTGATCGATCTGCCTTGATTTGGGGTGATGGTCATTGCAGAGACACCAAGGGCGGTGGAGCTGGGGACGTGAATTCTAGTCGGGATGGTAGTTTGGAGGCAATGCAGAAGAAAGAAGAGGTGAAGAAGTGGGTGCTTGAGAAGCTCCACGCGTGTTTTGGCGGTTTGAATGGGGATAATTATGCAAGGAGGTTGGATGGGGTGTCAGATGTGGAGATGTTTCATCTCACTTCAATGTGTTATGCATTTCAACTTGAATCGATCTCACACTGCGGTCCTGGGGAGTCGTACAAGTCCGGAAAATCGATTTGGGTTTCGGATGCCGGTAGTTGTTTACATCATTACCAGTCGAGGTCATTTTTAGCAAGATTGGCTGGGTTTCAGACAGTGGTGTTTGTACCGATGAAATCAGGAGTTGTGGAACTCGGTTCGGTCAAATCAACTTCGGAAGAACAAAGTTATGTGGATATGGTCAGAAGTGCATTTGGGGAGTCTAGTCCTATTCAGGCAAAGGCATTTCCAATGATATTTGGACGTGAACTAAGTCTCGGGGGTCCAAAATCACAATCCGTCAACATTTCCTTTACTCCAAAAGTAGAAGAAGATTTTGCATTTCCTCCAGAATCGTTTGAATTACAATCAGTAGGCACTTCAAATGGATGTCGAAGTGACGATAGTGAAGTAAAACTGTTTCCGCAAATGAACCAAATGATGGTTGACGGTTTCAATGTTCAGACAAGAGTCTCAAGTTCGGAGCTGCTCAAGGATGAGTCCTCGGCACAAATTGATGAGCGGAAACCCAGAAAGAGAGGGAGAAAGCCTGCCAATGGGAGAGAAGAACCATTGAATCATGTGGAAGCAGAACGGCAGAGGCGTGAGAAGCTTAACCAGAGGTTCTATGCACTAAGAGCTGTTGTTCCTAACATATCGAAGATGGATAAAGCCTCTCTTCTTGGTGATGCCATTACCTATATCACCGATCTACAGATGAAGATCAGAGTCATGGAAACTGAAAAGCAGATGGTAAACAACAAGGGAAAGCAGTTGCCGGTTCCAGAGATTGATTTTCAGGAACGCCTCGAGGATGCAGTTGTGAGGATGAACTGCCCATTGGATTCTCACCCGGTTTCTGAAGTCTTCAGAACACTTCGGGAACATAAAATTGTAGCTCAAGAGTCTAACGTTTCCATAACAGACAACGATAAGGTTATTCATACATTCTCCATTCAAACTCAAAGCGGTGATGCCGAGCAATTAAAGGAGAAGCTGGTGGCCTGTCTTTCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009892 GO:0010468 GO:0010556 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048519 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

503

Amino Acids

56.02

Weight (kDa)

6.02

Isoelectric Point (pI)

50.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	49 - 235	9.6e-46	bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH	PF00010	356 - 402	6.4e-12	Helix-loop-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006620)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16430
fragaria_vesca	FvH4_2g22150 FvH4_2g22150
malus_domestica	MD05G1202500.v1.1 MD05G1202900.v1.1 MD10G1190000.v1.1
prunus_persica	Prupe.8G228700_v2.0.a1 Prupe.8G228700_v2.0.a1 Prupe.8G228700_v2.0.a1 Prupe.8G228700_v2.0.a1
pyrus_communis	pycom05g18900 pycom05g18950 pycom10g16360 pycom10g16370
rosa_chinensis	RchiOBHm_Chr6g0288981
rosa_laevigata	RLG00000012333 RLG00000013932
rosa_multiflora	Rmu_sc0000666.1_g000010
rosa_roxburghii	Rroxscaffold_7G00179620
rosa_rugosa	Rorug06G0199900
rosa_samantha	Rh6AG311600 Rh6BG318100 Rh6CG325000 Rh6DG310800
rosa_wichuraiana	Rw6G013790 Rw6G026880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1298
Acc65I	GGTACC	1 cut(s)	59
AccB1I	GGYRCC	1 cut(s)	59
AccB7I	CCANNNNNTGG	2 cut(s)	198, 931
AccBSI	CCGCTC	1 cut(s)	1012
AccII	CGCG	1 cut(s)	371
AccIII	TCCGGA	1 cut(s)	515
AciI	CCGC	7 cut(s)	125, 273, 381, 492, 914, 1012, 1458
AclI	AACGTT	1 cut(s)	1401
AcsI	RAATTY	2 cut(s)	11, 292
AcuI	CTGAAG	2 cut(s)	1342, 1371
AfaI	GTAC	4 cut(s)	54, 61, 509, 618
AfiI	CCNNNNNNNGG	4 cut(s)	198, 240, 931, 1046
AflIII	ACRYGT	1 cut(s)	369
AjiI	CACGTC	2 cut(s)	289, 755
AjnI	CCWGG	1 cut(s)	496
AjuI	GAANNNNNNNTTGG	4 cut(s)	511, 543, 1313, 1345
AluBI	AGCT	9 cut(s)	68, 163, 281, 364, 976, 1102, 1130, 1388, 1486
AluI	AGCT	9 cut(s)	68, 163, 281, 364, 976, 1102, 1130, 1388, 1486
Alw26I	GTCTC	3 cut(s)	255, 770, 967
AlwNI	CAGNNNCTG	1 cut(s)	154
Ama87I	CYCGRG	2 cut(s)	767, 1298
Aor13HI	TCCGGA	1 cut(s)	515
AoxI	GGCC	2 cut(s)	122, 1491
ApeKI	GCWGC	2 cut(s)	145, 976
ApoI	RAATTY	2 cut(s)	11, 292
Asp718I	GGTACC	1 cut(s)	59
AspS9I	GGNCC	2 cut(s)	494, 773
AsuC2I	CCSGG	2 cut(s)	129, 1343
AsuHPI	GGTGA	7 cut(s)	17, 257, 352, 1186, 1187, 1331, 1472
AvaI	CYCGRG	2 cut(s)	767, 1298
AvaII	GGWCC	2 cut(s)	494, 773
BanI	GGYRCC	1 cut(s)	59
BbsI	GAAGAC	2 cut(s)	36, 1348
BbvI	GCAGC	2 cut(s)	157, 963
BccI	CCATC	7 cut(s)	37, 242, 299, 410, 925, 1147, 1234
BceAI	ACGGC	1 cut(s)	1100
BciT130I	CCWGG	1 cut(s)	498
BcnI	CCSGG	2 cut(s)	129, 1343
BcoDI	GTCTC	3 cut(s)	255, 770, 967
BfaI	CTAG	2 cut(s)	297, 714
BfmI	CTRYAG	1 cut(s)	1202
BisI	GCNGC	3 cut(s)	125, 146, 977
BlsI	GCNGC	3 cut(s)	126, 147, 978
BmcAI	AGTACT	1 cut(s)	54
Bme1390I	CCNGG	3 cut(s)	129, 498, 1343
Bme18I	GGWCC	2 cut(s)	494, 773
BmeT110I	CYCGRG	2 cut(s)	767, 1298
BmgBI	CACGTC	2 cut(s)	289, 755
BmgT120I	GGNCC	2 cut(s)	494, 773
BmiI	GGNNCC	3 cut(s)	61, 774, 1272
BmrFI	CCNGG	3 cut(s)	129, 498, 1343
BmrI	ACTGGG	1 cut(s)	65
BmsI	GCATC	4 cut(s)	529, 1168, 1294, 1453
BmuI	ACTGGG	1 cut(s)	65
BoxI	GACNNNNGTC	1 cut(s)	959
BpiI	GAAGAC	2 cut(s)	36, 1348
BpmI	CTGGAG	1 cut(s)	822
BpuEI	CTTGAG	4 cut(s)	377, 949, 965, 1374
BpuMI	CCSGG	2 cut(s)	129, 1343
Bsa29I	ATCGAT	3 cut(s)	226, 479, 524
BsaBI	GATNNNNATC	1 cut(s)	1211
BsaJI	CCNNGG	3 cut(s)	267, 497, 993
BsaWI	WCCGGW	1 cut(s)	515
Bsc4I	CCNNNNNNNGG	4 cut(s)	198, 240, 931, 1046
Bse118I	RCCGGY	2 cut(s)	542, 1267
Bse1I	ACTGG	4 cut(s)	60, 73, 93, 565
Bse3DI	GCAATG	2 cut(s)	253, 327
Bse8I	GATNNNNATC	1 cut(s)	1211
BseAI	TCCGGA	1 cut(s)	515
BseBI	CCWGG	1 cut(s)	498
BseCI	ATCGAT	3 cut(s)	226, 479, 524
BseDI	CCNNGG	3 cut(s)	267, 497, 993
BseGI	GGATG	7 cut(s)	310, 421, 544, 884, 991, 1309, 1323
BseJI	GATNNNNATC	1 cut(s)	1211
BseLI	CCNNNNNNNGG	4 cut(s)	198, 240, 931, 1046
BseMI	GCAATG	2 cut(s)	253, 327
BseNI	ACTGG	4 cut(s)	60, 73, 93, 565
BseXI	GCAGC	2 cut(s)	157, 963
BseYI	CCCAGC	2 cut(s)	281, 593
BsgI	GTGCAG	1 cut(s)	164
Bsh1236I	CGCG	1 cut(s)	371
BshFI	GGCC	2 cut(s)	124, 1493
BshNI	GGYRCC	1 cut(s)	59
BshVI	ATCGAT	3 cut(s)	226, 479, 524
BsiHKCI	CYCGRG	2 cut(s)	767, 1298
BsiSI	CCGG	5 cut(s)	128, 516, 543, 1268, 1343
BslFI	GGGAC	1 cut(s)	299
BslI	CCNNNNNNNGG	4 cut(s)	198, 240, 931, 1046
BsmAI	GTCTC	3 cut(s)	255, 770, 967
BsmFI	GGGAC	1 cut(s)	299
BsnI	GGCC	2 cut(s)	124, 1493
BsoBI	CYCGRG	2 cut(s)	767, 1298
Bsp13I	TCCGGA	1 cut(s)	515
Bsp143I	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
BspACI	CCGC	7 cut(s)	125, 273, 381, 492, 914, 1012, 1458
BspANI	GGCC	2 cut(s)	124, 1493
BspDI	ATCGAT	3 cut(s)	226, 479, 524
BspEI	TCCGGA	1 cut(s)	515
BspFNI	CGCG	1 cut(s)	371
BspLI	GGNNCC	3 cut(s)	61, 774, 1272
BspT107I	GGYRCC	1 cut(s)	59
BsrBI	CCGCTC	1 cut(s)	1012
BsrDI	GCAATG	2 cut(s)	253, 327
BsrFI	RCCGGY	2 cut(s)	542, 1267
BsrI	ACTGG	4 cut(s)	60, 73, 93, 565
BssAI	RCCGGY	2 cut(s)	542, 1267
BssECI	CCNNGG	3 cut(s)	267, 497, 993
BssMI	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
BssT1I	CCWWGG	1 cut(s)	267
Bst2UI	CCWGG	1 cut(s)	498
Bst4CI	ACNGT	3 cut(s)	607, 909, 941
Bst6I	CTCTTC	2 cut(s)	330, 1173
BstC8I	GCNNGC	4 cut(s)	70, 161, 204, 1041
BstDEI	CTNAG	2 cut(s)	761, 1124
BstF5I	GGATG	7 cut(s)	310, 421, 544, 884, 991, 1309, 1323
BstFNI	CGCG	1 cut(s)	371
BstKTI	GATC	6 cut(s)	84, 226, 230, 483, 1201, 1215
BstMAI	GTCTC	3 cut(s)	255, 770, 967
BstMBI	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
BstMWI	GCNNNNNNNGC	4 cut(s)	361, 590, 1099, 1127
BstNI	CCWGG	1 cut(s)	498
BstPAI	GACNNNNGTC	1 cut(s)	959
BstSCI	CCNGG	3 cut(s)	127, 496, 1341
BstSFI	CTRYAG	1 cut(s)	1202
BstUI	CGCG	1 cut(s)	371
BstV1I	GCAGC	2 cut(s)	157, 963
BstV2I	GAAGAC	2 cut(s)	36, 1348
Bsu15I	ATCGAT	3 cut(s)	226, 479, 524
BsuRI	GGCC	2 cut(s)	124, 1493
BsuTUI	ATCGAT	3 cut(s)	226, 479, 524
BtrI	CACGTC	2 cut(s)	289, 755
BtsCI	GGATG	7 cut(s)	310, 421, 544, 884, 991, 1309, 1323
BtsI	GCAGTG	1 cut(s)	487
BtsIMutI	CAGTG	3 cut(s)	86, 487, 612
Cac8I	GCNNGC	4 cut(s)	70, 161, 204, 1041
CaiI	CAGNNNCTG	1 cut(s)	154
Cfr10I	RCCGGY	2 cut(s)	542, 1267
Cfr13I	GGNCC	2 cut(s)	494, 773
ClaI	ATCGAT	3 cut(s)	226, 479, 524
Csp6I	GTAC	4 cut(s)	53, 60, 508, 617
CspCI	CAANNNNNGTGG	2 cut(s)	256, 291
CviAII	CATG	2 cut(s)	1070, 1222
CviQI	GTAC	4 cut(s)	53, 60, 508, 617
DdeI	CTNAG	2 cut(s)	761, 1124
DpnI	GATC	6 cut(s)	83, 225, 229, 482, 1200, 1214
DpnII	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
Eam1104I	CTCTTC	2 cut(s)	330, 1173
EarI	CTCTTC	2 cut(s)	330, 1173
Eco130I	CCWWGG	1 cut(s)	267
Eco47I	GGWCC	2 cut(s)	494, 773
Eco57I	CTGAAG	2 cut(s)	1342, 1371
Eco88I	CYCGRG	2 cut(s)	767, 1298
EcoRI	GAATTC	1 cut(s)	292
EcoRII	CCWGG	1 cut(s)	496
EcoT14I	CCWWGG	1 cut(s)	267
EcoT22I	ATGCAT	1 cut(s)	466
ErhI	CCWWGG	1 cut(s)	267
FaeI	CATG	2 cut(s)	1073, 1225
FalI	AAGNNNNNCTT	2 cut(s)	1152, 1184
FaqI	GGGAC	1 cut(s)	299
FatI	CATG	2 cut(s)	1069, 1221
Fnu4HI	GCNGC	3 cut(s)	125, 146, 977
FokI	GGATG	7 cut(s)	317, 428, 551, 891, 998, 1316, 1330
Fsp4HI	GCNGC	3 cut(s)	125, 146, 977
FspBI	CTAG	2 cut(s)	297, 714
GluI	GCNGC	3 cut(s)	125, 146, 977
GsaI	CCCAGC	2 cut(s)	285, 597
GsuI	CTGGAG	1 cut(s)	822
HaeIII	GGCC	2 cut(s)	124, 1493
HapII	CCGG	5 cut(s)	128, 516, 543, 1268, 1343
Hin1II	CATG	2 cut(s)	1073, 1225
HincII	GTYRAC	2 cut(s)	793, 937
HindII	GTYRAC	2 cut(s)	793, 937
HindIII	AAGCTT	2 cut(s)	66, 1100
HpaII	CCGG	5 cut(s)	128, 516, 543, 1268, 1343
HphI	GGTGA	7 cut(s)	17, 257, 352, 1186, 1187, 1331, 1472
Hpy166II	GTNNAC	5 cut(s)	554, 758, 793, 937, 1246
Hpy8I	GTNNAC	5 cut(s)	554, 758, 793, 937, 1246
HpyCH4III	ACNGT	3 cut(s)	607, 909, 941
HpyCH4IV	ACGT	3 cut(s)	288, 754, 1401
HpyCH4V	TGCA	9 cut(s)	145, 258, 325, 404, 464, 701, 830, 1121, 1307
HpyF10VI	GCNNNNNNNGC	4 cut(s)	361, 590, 1099, 1127
HpyF3I	CTNAG	2 cut(s)	761, 1124
HpySE526I	ACGT	3 cut(s)	288, 754, 1401
Hsp92II	CATG	2 cut(s)	1073, 1225
Kpn2I	TCCGGA	1 cut(s)	515
KpnI	GGTACC	1 cut(s)	63
Kzo9I	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
LmnI	GCTCC	3 cut(s)	278, 369, 973
Lsp1109I	GCAGC	2 cut(s)	157, 963
LweI	GCATC	4 cut(s)	529, 1168, 1294, 1453
MaeI	CTAG	2 cut(s)	297, 714
MaeII	ACGT	3 cut(s)	288, 754, 1401
MaeIII	GTNAC	1 cut(s)	887
MalI	GATC	6 cut(s)	83, 225, 229, 482, 1200, 1214
MbiI	CCGCTC	1 cut(s)	1012
MboI	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
MluI	ACGCGT	1 cut(s)	369
MlyI	GAGTC	7 cut(s)	56, 512, 719, 969, 998, 1227, 1403
MmeI	TCCRAC	1 cut(s)	393
Mph1103I	ATGCAT	1 cut(s)	466
MroI	TCCGGA	1 cut(s)	515
MseI	TTAA	2 cut(s)	1104, 1475
MslI	CAYNNNNRTG	1 cut(s)	452
MspI	CCGG	5 cut(s)	128, 516, 543, 1268, 1343
MspR9I	CCNGG	3 cut(s)	129, 498, 1343
MvaI	CCWGG	1 cut(s)	498
MvnI	CGCG	1 cut(s)	371
MwoI	GCNNNNNNNGC	4 cut(s)	361, 590, 1099, 1127
NciI	CCSGG	2 cut(s)	129, 1343
NdeII	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
NlaIII	CATG	2 cut(s)	1073, 1225
NlaIV	GGNNCC	3 cut(s)	61, 774, 1272
NmeAIII	GCCGAG	2 cut(s)	974, 1492
NmuCI	GTSAC	1 cut(s)	887
NsiI	ATGCAT	1 cut(s)	466
PaeR7I	CTCGAG	1 cut(s)	1298
PcsI	WCGNNNNNNNCGW	1 cut(s)	1090
PfeI	GAWTC	4 cut(s)	476, 842, 1066, 1334
PflFI	GACNNNGTC	1 cut(s)	35
PflMI	CCANNNNNTGG	2 cut(s)	198, 931
PkrI	GCNGC	3 cut(s)	126, 147, 978
PleI	GAGTC	7 cut(s)	55, 511, 718, 968, 997, 1226, 1402
PpsI	GAGTC	7 cut(s)	55, 511, 718, 968, 997, 1226, 1402
PshAI	GACNNNNGTC	1 cut(s)	959
Psp1406I	AACGTT	1 cut(s)	1401
Psp6I	CCWGG	1 cut(s)	496
PspFI	CCCAGC	2 cut(s)	281, 593
PspGI	CCWGG	1 cut(s)	496
PspN4I	GGNNCC	3 cut(s)	61, 774, 1272
PspPI	GGNCC	2 cut(s)	494, 773
PspXI	VCTCGAGB	1 cut(s)	1298
PstNI	CAGNNNCTG	1 cut(s)	154
PsyI	GACNNNGTC	1 cut(s)	35
RsaI	GTAC	4 cut(s)	54, 61, 509, 618
RsaNI	GTAC	4 cut(s)	53, 60, 508, 617
RseI	CAYNNNNRTG	1 cut(s)	452
SaqAI	TTAA	2 cut(s)	1104, 1475
SatI	GCNGC	3 cut(s)	125, 146, 977
Sau3AI	GATC	6 cut(s)	81, 223, 227, 480, 1198, 1212
Sau96I	GGNCC	2 cut(s)	494, 773
ScaI	AGTACT	1 cut(s)	54
SchI	GAGTC	7 cut(s)	56, 512, 719, 969, 998, 1227, 1403
ScrFI	CCNGG	3 cut(s)	129, 498, 1343
SfaNI	GCATC	4 cut(s)	529, 1168, 1294, 1453
SfcI	CTRYAG	1 cut(s)	1202
Sfr274I	CTCGAG	1 cut(s)	1298
SinI	GGWCC	2 cut(s)	494, 773
SlaI	CTCGAG	1 cut(s)	1298
SmiMI	CAYNNNNRTG	1 cut(s)	452
SmlI	CTYRAG	5 cut(s)	356, 964, 980, 1298, 1389
SmoI	CTYRAG	5 cut(s)	356, 964, 980, 1298, 1389
SsiI	CCGC	7 cut(s)	125, 273, 381, 492, 914, 1012, 1458
SspMI	CTAG	2 cut(s)	297, 714
StyD4I	CCNGG	3 cut(s)	127, 496, 1341
StyI	CCWWGG	1 cut(s)	267
TaaI	ACNGT	3 cut(s)	607, 909, 941
TaiI	ACGT	3 cut(s)	291, 757, 1404
TaqI	TCGA	8 cut(s)	176, 226, 479, 524, 569, 883, 1148, 1299
TaqII	GACCGA	1 cut(s)	640
TatI	WGTACW	1 cut(s)	52
TauI	GCSGC	1 cut(s)	127
TfiI	GAWTC	4 cut(s)	476, 842, 1066, 1334
Tru1I	TTAA	2 cut(s)	1104, 1475
Tru9I	TTAA	2 cut(s)	1104, 1475
TscAI	CASTG	3 cut(s)	93, 494, 612
TseFI	GTSAC	1 cut(s)	887
TseI	GCWGC	2 cut(s)	145, 976
Tsp45I	GTSAC	1 cut(s)	887
TspDTI	ATGAA	7 cut(s)	39, 431, 638, 935, 1223, 1334, 1421
TspGWI	ACGGA	1 cut(s)	778
TspRI	CASTG	3 cut(s)	93, 494, 612
Tth111I	GACNNNGTC	1 cut(s)	35
Van91I	CCANNNNNTGG	2 cut(s)	198, 931
VpaK11BI	GGWCC	2 cut(s)	494, 773
XapI	RAATTY	2 cut(s)	11, 292
XcmI	CCANNNNNNNNNTGG	2 cut(s)	167, 374
XhoI	CTCGAG	1 cut(s)	1298
XspI	CTAG	2 cut(s)	297, 714
ZrmI	AGTACT	1 cut(s)	54
Zsp2I	ATGCAT	1 cut(s)	466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.