FvH4_2g22330

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0004291
Physical Location & Seq
Forward (+)
18335758 .. 18337588
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22330.t3

Sequence Viewer

Length: 603 bp
ATGTCTTCCAATTCCAAATCCCCCTACGGCACCCTCCCTCCCACCGCCGCCGCCGACTCGTTCCCAACCCCATCCACCACCTTCTCACGCGCCAGGAGCGTCACCAGCTCCTACTTCGCCACGCGCCGCCCATGGCTCGAGCTCATCCAGCCCTTCTCCAGCTTCACGCGCCCCTTCACGTTCGGCGAGGCCACGCTCCGCATCAAGAGCAACCTCAACCACTTCCGCGTCAACTACGCCATGGCTGTCCTCTTCATCCTCTTCCTCAGCCTCCTCTGGCACCCCGTCTCCCTCATCGTCTTCCTCCTCGTCTTCGTCGCCTGGTTCTTCCTCTACTTCTTCCGCGACGGGCCCCTCGTCCTCTTCAACTGGATCGTCGACGACCGCATCGTCCTCGCCGTCCTCGCCGTCGTCACAATCGTCGCTCTCGTCTTCACTCACGTCTGGCTCAACGTCCTCGTCTCCCTTCTCATCGCCGTCGCTCTCGTGCTGCTGCACGCGGCGTTTCGCGGCACTGAGGATTTGTATTCCGAGGACCTTGAAGGCGGCGGCTTGTTCTCCGTCGTCGGCAGTCCCACGCGAAACGGCTACACCGGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

201

Amino Acids

22.38

Weight (kDa)

9.15

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 39 - 177 3.3e-46 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AccB1I GGYRCC 2 cut(s) 29, 279
AccI GTMKAC 1 cut(s) 378
AccII CGCG 8 cut(s) 90, 124, 169, 228, 345, 500, 510, 580
AclWI GGATC 1 cut(s) 380
AgsI TTSAA 2 cut(s) 367, 542
AjiI CACGTC 1 cut(s) 442
AjnI CCWGG 2 cut(s) 92, 320
AluBI AGCT 3 cut(s) 108, 142, 162
AluI AGCT 3 cut(s) 108, 142, 162
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 292, 466
AlwI GGATC 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 2 cut(s) 189, 350
ApaI GGGCCC 1 cut(s) 354
ApeKI GCWGC 2 cut(s) 490, 493
AspLEI GCGC 3 cut(s) 92, 126, 171
AspS9I GGNCC 3 cut(s) 350, 351, 535
AsuHPI GGTGA 1 cut(s) 94
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 535
BaeGI GKGCMC 1 cut(s) 354
BanI GGYRCC 2 cut(s) 29, 279
BanII GRGCYC 2 cut(s) 144, 354
BauI CACGAG 1 cut(s) 485
BbsI GAAGAC 3 cut(s) 292, 304, 424
Bbv12I GWGCWC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 2 cut(s) 477, 480
BccI CCATC 1 cut(s) 79
BceAI ACGGC 5 cut(s) 43, 383, 392, 461, 601
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BciT130I CCWGG 2 cut(s) 94, 322
BcoDI GTCTC 2 cut(s) 292, 466
BisI GCNGC 9 cut(s) 48, 51, 127, 491, 494, 501, 511, 547, 550
BlsI GCNGC 9 cut(s) 49, 52, 128, 492, 495, 502, 512, 548, 551
Bme1390I CCNGG 2 cut(s) 94, 322
Bme18I GGWCC 1 cut(s) 535
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 3 cut(s) 350, 351, 535
BmiI GGNNCC 4 cut(s) 31, 281, 352, 353
BmrFI CCNGG 2 cut(s) 94, 322
BmsI GCATC 2 cut(s) 210, 396
BpiI GAAGAC 3 cut(s) 292, 304, 424
BpmI CTGGAG 1 cut(s) 142
Bpu10I CCTNAGC 1 cut(s) 266
BsaJI CCNNGG 3 cut(s) 131, 240, 531
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 272, 302
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 374
BseBI CCWGG 2 cut(s) 94, 322
BseDI CCNNGG 3 cut(s) 131, 240, 531
BseGI GGATG 3 cut(s) 71, 144, 255
BseMII CTCAG 2 cut(s) 280, 507
BseNI ACTGG 1 cut(s) 374
BseRI GAGGAG 2 cut(s) 263, 296
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 2 cut(s) 477, 480
BsgI GTGCAG 1 cut(s) 479
Bsh1236I CGCG 8 cut(s) 90, 124, 169, 228, 345, 500, 510, 580
Bsh1285I CGRYCG 1 cut(s) 385
BshFI GGCC 2 cut(s) 191, 352
BshNI GGYRCC 2 cut(s) 29, 279
BsiEI CGRYCG 1 cut(s) 385
BsiHKAI GWGCWC 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 594
BslFI GGGAC 1 cut(s) 558
BsmAI GTCTC 2 cut(s) 292, 466
BsmBI CGTCTC 2 cut(s) 292, 466
BsmFI GGGAC 1 cut(s) 558
BsnI GGCC 2 cut(s) 191, 352
BsoBI CYCGRG 1 cut(s) 137
Bsp120I GGGCCC 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 144, 354
Bsp143I GATC 1 cut(s) 372
Bsp19I CCATGG 2 cut(s) 131, 240
BspANI GGCC 2 cut(s) 191, 352
BspCNI CTCAG 2 cut(s) 279, 508
BspFNI CGCG 8 cut(s) 90, 124, 169, 228, 345, 500, 510, 580
BspLI GGNNCC 4 cut(s) 31, 281, 352, 353
BspPI GGATC 1 cut(s) 380
BspT107I GGYRCC 2 cut(s) 29, 279
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 1 cut(s) 374
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 3 cut(s) 131, 240, 531
BssMI GATC 1 cut(s) 372
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 2 cut(s) 131, 240
Bst2BI CACGAG 1 cut(s) 485
Bst2UI CCWGG 2 cut(s) 94, 322
Bst6I CTCTTC 3 cut(s) 257, 266, 368
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 2 cut(s) 266, 516
BstDSI CCRYGG 2 cut(s) 131, 240
BstF5I GGATG 3 cut(s) 71, 144, 255
BstFNI CGCG 8 cut(s) 90, 124, 169, 228, 345, 500, 510, 580
BstHHI GCGC 3 cut(s) 92, 126, 171
BstKTI GATC 1 cut(s) 375
BstMAI GTCTC 2 cut(s) 292, 466
BstMBI GATC 1 cut(s) 372
BstMCI CGRYCG 1 cut(s) 385
BstMWI GCNNNNNNNGC 7 cut(s) 96, 105, 148, 168, 207, 404, 594
BstNI CCWGG 2 cut(s) 94, 322
BstSCI CCNGG 2 cut(s) 92, 320
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 8 cut(s) 90, 124, 169, 228, 345, 500, 510, 580
BstV1I GCAGC 2 cut(s) 477, 480
BstV2I GAAGAC 3 cut(s) 292, 304, 424
BsuRI GGCC 2 cut(s) 191, 352
BtgI CCRYGG 2 cut(s) 131, 240
BtgZI GCGATG 1 cut(s) 457
BtrI CACGTC 1 cut(s) 442
BtsCI GGATG 3 cut(s) 71, 144, 255
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 1 cut(s) 498
CfoI GCGC 3 cut(s) 92, 126, 171
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 3 cut(s) 350, 351, 535
CseI GACGC 2 cut(s) 88, 217
CviAII CATG 2 cut(s) 132, 241
DdeI CTNAG 2 cut(s) 266, 516
DpnI GATC 1 cut(s) 374
DpnII GATC 1 cut(s) 372
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 3 cut(s) 257, 266, 368
EarI CTCTTC 3 cut(s) 257, 266, 368
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 131, 240
Eco24I GRGCYC 2 cut(s) 144, 354
Eco47I GGWCC 1 cut(s) 535
Eco53kI GAGCTC 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 137
EcoICRI GAGCTC 1 cut(s) 142
EcoO109I RGGNCCY 2 cut(s) 351, 535
EcoRII CCWGG 2 cut(s) 92, 320
EcoT14I CCWWGG 2 cut(s) 131, 240
EcoT38I GRGCYC 2 cut(s) 144, 354
ErhI CCWWGG 2 cut(s) 131, 240
Esp3I CGTCTC 2 cut(s) 292, 466
FaeI CATG 2 cut(s) 135, 244
FaiI YATR 2 cut(s) 133, 242
FaqI GGGAC 1 cut(s) 558
FatI CATG 2 cut(s) 131, 240
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 9 cut(s) 48, 51, 127, 491, 494, 501, 511, 547, 550
FokI GGATG 3 cut(s) 58, 131, 242
FriOI GRGCYC 2 cut(s) 144, 354
Fsp4HI GCNGC 9 cut(s) 48, 51, 127, 491, 494, 501, 511, 547, 550
GlaI GCGC 3 cut(s) 91, 125, 170
GluI GCNGC 9 cut(s) 48, 51, 127, 491, 494, 501, 511, 547, 550
GsuI CTGGAG 1 cut(s) 142
HaeIII GGCC 2 cut(s) 191, 352
HapII CCGG 1 cut(s) 594
HgaI GACGC 2 cut(s) 88, 217
HhaI GCGC 3 cut(s) 92, 126, 171
Hin1II CATG 2 cut(s) 135, 244
Hin6I GCGC 3 cut(s) 90, 124, 169
HinP1I GCGC 3 cut(s) 90, 124, 169
HincII GTYRAC 2 cut(s) 232, 379
HindII GTYRAC 2 cut(s) 232, 379
HinfI GANTC 1 cut(s) 56
HpaII CCGG 1 cut(s) 594
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 232, 379
Hpy188I TCNGA 1 cut(s) 532
Hpy188III TCNNGA 1 cut(s) 205
Hpy8I GTNNAC 2 cut(s) 232, 379
Hpy99I CGWCG 9 cut(s) 320, 350, 380, 383, 413, 425, 482, 566, 569
HpyAV CCTTC 5 cut(s) 91, 163, 184, 476, 536
HpyCH4IV ACGT 3 cut(s) 179, 441, 453
HpyCH4V TGCA 1 cut(s) 496
HpyF10VI GCNNNNNNNGC 7 cut(s) 96, 105, 148, 168, 207, 404, 594
HpyF3I CTNAG 2 cut(s) 266, 516
HpySE526I ACGT 3 cut(s) 179, 441, 453
Hsp92II CATG 2 cut(s) 135, 244
HspAI GCGC 3 cut(s) 90, 124, 169
Kzo9I GATC 1 cut(s) 372
LmnI GCTCC 3 cut(s) 96, 113, 201
Lsp1109I GCAGC 2 cut(s) 477, 480
LweI GCATC 2 cut(s) 210, 396
MaeII ACGT 3 cut(s) 179, 441, 453
MaeIII GTNAC 2 cut(s) 100, 412
MalI GATC 1 cut(s) 374
MboI GATC 1 cut(s) 372
MboII GAAGA 8 cut(s) 244, 253, 292, 304, 319, 331, 355, 424
MhlI GDGCHC 2 cut(s) 144, 354
MluCI AATT 1 cut(s) 10
MlyI GAGTC 1 cut(s) 50
MspI CCGG 1 cut(s) 594
MspR9I CCNGG 2 cut(s) 94, 322
MvaI CCWGG 2 cut(s) 94, 322
MvnI CGCG 8 cut(s) 90, 124, 169, 228, 345, 500, 510, 580
MwoI GCNNNNNNNGC 7 cut(s) 96, 105, 148, 168, 207, 404, 594
NcoI CCATGG 2 cut(s) 131, 240
NdeII GATC 1 cut(s) 372
NlaIII CATG 2 cut(s) 135, 244
NlaIV GGNNCC 4 cut(s) 31, 281, 352, 353
NmuCI GTSAC 2 cut(s) 100, 412
PaeR7I CTCGAG 1 cut(s) 137
PcsI WCGNNNNNNNCGW 5 cut(s) 354, 387, 396, 417, 426
PkrI GCNGC 9 cut(s) 49, 52, 128, 492, 495, 502, 512, 548, 551
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 535
Psp124BI GAGCTC 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 2 cut(s) 92, 320
PspGI CCWGG 2 cut(s) 92, 320
PspN4I GGNNCC 4 cut(s) 31, 281, 352, 353
PspOMI GGGCCC 1 cut(s) 350
PspPI GGNCC 3 cut(s) 350, 351, 535
PspPPI RGGWCCY 1 cut(s) 535
PspXI VCTCGAGB 1 cut(s) 137
SacI GAGCTC 1 cut(s) 144
SalI GTCGAC 1 cut(s) 377
SatI GCNGC 9 cut(s) 48, 51, 127, 491, 494, 501, 511, 547, 550
Sau3AI GATC 1 cut(s) 372
Sau96I GGNCC 3 cut(s) 350, 351, 535
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 2 cut(s) 94, 322
SduI GDGCHC 2 cut(s) 144, 354
SetI ASST 9 cut(s) 83, 110, 144, 164, 182, 216, 444, 456, 540
SfaNI GCATC 2 cut(s) 210, 396
Sfr274I CTCGAG 1 cut(s) 137
SgrDI CGTCGACG 1 cut(s) 377
SinI GGWCC 1 cut(s) 535
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 1 cut(s) 10
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 2 cut(s) 92, 320
StyI CCWWGG 2 cut(s) 131, 240
TaiI ACGT 3 cut(s) 182, 444, 456
TaqI TCGA 2 cut(s) 138, 378
TasI AATT 1 cut(s) 10
TauI GCSGC 7 cut(s) 50, 53, 129, 503, 513, 549, 552
TscAI CASTG 1 cut(s) 520
TseFI GTSAC 2 cut(s) 100, 412
TseI GCWGC 2 cut(s) 490, 493
Tsp45I GTSAC 2 cut(s) 100, 412
TspDTI ATGAA 1 cut(s) 244
TspGWI ACGGA 1 cut(s) 550
TspRI CASTG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 535
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.