RLG00000012311

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004291
Physical Location & Seq
Reverse (-)
17397898 .. 17399561
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012311

Sequence Viewer

Length: 603 bp
ATGTCTTCCTCCAATTCCAAAGCCGCCCCCTACGGCACCCTCCCTCCCCCCGCCGCCGCGTACACAACCCCAACCACGACGTTCTCACGCGCCAGAGACGCCACACAGGCCGTCTTCGCCACGCGCCGCCCATGGCTCGAGCTGGTCCAGCCTTTCTCCAGCTTCACGCGCCCCGACACTTTTGGCATGGCCACGCTCCGAATCAAGACCAACCTCAACCACTTCCGGGTCAACTACGCCATGGCCGTCCTCTTCATCCTCTTCCTCAGCCTCCTCTGGCACCCCGTCTCCCTCATCGTCTTCCTCCTCGTCTTCGTCGCCTGGTTCTTCCTCTACTTCTTCCGCGACGCTCCCCTCGTCCTCTTCAATTGGATCATCGACGACCGCATCGTCCTCGCCGTCCTCGCCCTCGTCACCATCGTCGCTCTCGTCTTCACCAGCGTCTGGTTGAACGTCCTCGTCTCCATTCTCGTTGGGATCGCGCTCGTGCTGTTACACGCCGCCTTTCGGGGCTTTGAGGATTTGTATAGGGAGGACCTTGAAGGCGGTGGCTTGTTTTCGGTCGTGGGCAGCCCCACCCGTAATGGCTACACCGGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

201

Amino Acids

22.38

Weight (kDa)

9.51

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 39 - 178 1.4e-45 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AccB1I GGYRCC 2 cut(s) 35, 279
AccB7I CCANNNNNTGG 1 cut(s) 444
AccII CGCG 6 cut(s) 59, 90, 124, 169, 345, 482
AciI CCGC 9 cut(s) 24, 51, 54, 57, 127, 343, 385, 501, 546
AclWI GGATC 2 cut(s) 380, 485
AcoI YGGCCR 2 cut(s) 189, 243
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 3 cut(s) 226, 444, 507
AgsI TTSAA 3 cut(s) 367, 451, 542
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 2 cut(s) 142, 162
AluI AGCT 2 cut(s) 142, 162
Alw26I GTCTC 3 cut(s) 90, 292, 466
AlwI GGATC 2 cut(s) 380, 485
AlwNI CAGNNNCTG 1 cut(s) 444
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 3 cut(s) 108, 189, 243
ApeKI GCWGC 1 cut(s) 570
AspLEI GCGC 4 cut(s) 92, 126, 171, 484
AspS9I GGNCC 2 cut(s) 145, 535
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 406, 427
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 2 cut(s) 145, 535
BalI TGGCCA 1 cut(s) 191
BanI GGYRCC 2 cut(s) 35, 279
BauI CACGAG 1 cut(s) 485
BbsI GAAGAC 4 cut(s) 106, 292, 304, 424
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 1 cut(s) 582
BccI CCATC 1 cut(s) 425
BceAI ACGGC 4 cut(s) 49, 95, 230, 383
BcgI CGANNNNNNTGC 2 cut(s) 376, 410
BciT130I CCWGG 1 cut(s) 322
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 90, 292, 466
BfaI CTAG 1 cut(s) 601
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 6 cut(s) 24, 54, 57, 127, 501, 571
BlsI GCNGC 6 cut(s) 25, 55, 58, 128, 502, 572
Bme1390I CCNGG 2 cut(s) 227, 322
Bme18I GGWCC 2 cut(s) 145, 535
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 145, 535
BmiI GGNNCC 2 cut(s) 37, 281
BmrFI CCNGG 2 cut(s) 227, 322
BmsI GCATC 1 cut(s) 396
BpiI GAAGAC 4 cut(s) 106, 292, 304, 424
BpmI CTGGAG 1 cut(s) 142
Bpu10I CCTNAGC 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 227
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 131, 240
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 444, 507
Bse118I RCCGGY 1 cut(s) 593
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 131, 240
BseGI GGATG 1 cut(s) 255
BseLI CCNNNNNNNGG 3 cut(s) 226, 444, 507
BseMII CTCAG 1 cut(s) 280
BseRI GAGGAG 2 cut(s) 263, 296
BseXI GCAGC 1 cut(s) 582
Bsh1236I CGCG 6 cut(s) 59, 90, 124, 169, 345, 482
Bsh1285I CGRYCG 2 cut(s) 385, 564
BshFI GGCC 3 cut(s) 110, 191, 245
BshNI GGYRCC 2 cut(s) 35, 279
BsiEI CGRYCG 2 cut(s) 385, 564
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 2 cut(s) 226, 594
BslI CCNNNNNNNGG 3 cut(s) 226, 444, 507
BsmAI GTCTC 3 cut(s) 90, 292, 466
BsmBI CGTCTC 3 cut(s) 90, 292, 466
BsnI GGCC 3 cut(s) 110, 191, 245
BsoBI CYCGRG 1 cut(s) 137
Bsp143I GATC 2 cut(s) 372, 477
Bsp19I CCATGG 2 cut(s) 131, 240
BspACI CCGC 9 cut(s) 24, 51, 54, 57, 127, 343, 385, 501, 546
BspANI GGCC 3 cut(s) 110, 191, 245
BspCNI CTCAG 1 cut(s) 279
BspFNI CGCG 6 cut(s) 59, 90, 124, 169, 345, 482
BspLI GGNNCC 2 cut(s) 37, 281
BspPI GGATC 2 cut(s) 380, 485
BspT107I GGYRCC 2 cut(s) 35, 279
BsrFI RCCGGY 1 cut(s) 593
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 2 cut(s) 131, 240
BssMI GATC 2 cut(s) 372, 477
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 2 cut(s) 131, 240
Bst2BI CACGAG 1 cut(s) 485
Bst2UI CCWGG 1 cut(s) 322
Bst6I CTCTTC 3 cut(s) 257, 266, 368
BstACI GRCGYC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 266
BstDSI CCRYGG 2 cut(s) 131, 240
BstF5I GGATG 1 cut(s) 255
BstFNI CGCG 6 cut(s) 59, 90, 124, 169, 345, 482
BstHHI GCGC 4 cut(s) 92, 126, 171, 484
BstKTI GATC 2 cut(s) 375, 480
BstMAI GTCTC 3 cut(s) 90, 292, 466
BstMBI GATC 2 cut(s) 372, 477
BstMCI CGRYCG 2 cut(s) 385, 564
BstMWI GCNNNNNNNGC 7 cut(s) 98, 107, 116, 148, 168, 404, 594
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 225, 320
BstUI CGCG 6 cut(s) 59, 90, 124, 169, 345, 482
BstV1I GCAGC 1 cut(s) 582
BstV2I GAAGAC 4 cut(s) 106, 292, 304, 424
BsuRI GGCC 3 cut(s) 110, 191, 245
BtgI CCRYGG 2 cut(s) 131, 240
BtsCI GGATG 1 cut(s) 255
CaiI CAGNNNCTG 1 cut(s) 444
CfoI GCGC 4 cut(s) 92, 126, 171, 484
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 2 cut(s) 145, 535
CseI GACGC 3 cut(s) 107, 356, 430
Csp6I GTAC 1 cut(s) 61
CviAII CATG 3 cut(s) 132, 187, 241
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 1 cut(s) 266
DpnI GATC 2 cut(s) 374, 479
DpnII GATC 2 cut(s) 372, 477
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 2 cut(s) 189, 243
Eam1104I CTCTTC 3 cut(s) 257, 266, 368
EarI CTCTTC 3 cut(s) 257, 266, 368
Eco130I CCWWGG 2 cut(s) 131, 240
Eco47I GGWCC 2 cut(s) 145, 535
Eco88I CYCGRG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 2 cut(s) 131, 240
ErhI CCWWGG 2 cut(s) 131, 240
Esp3I CGTCTC 3 cut(s) 90, 292, 466
FaeI CATG 3 cut(s) 135, 190, 244
FaiI YATR 4 cut(s) 133, 188, 242, 528
FatI CATG 3 cut(s) 131, 186, 240
FauI CCCGC 1 cut(s) 58
Fnu4HI GCNGC 6 cut(s) 24, 54, 57, 127, 501, 571
FokI GGATG 1 cut(s) 242
Fsp4HI GCNGC 6 cut(s) 24, 54, 57, 127, 501, 571
FspBI CTAG 1 cut(s) 601
GlaI GCGC 4 cut(s) 91, 125, 170, 483
GluI GCNGC 6 cut(s) 24, 54, 57, 127, 501, 571
GsuI CTGGAG 1 cut(s) 142
HaeIII GGCC 3 cut(s) 110, 191, 245
HapII CCGG 2 cut(s) 226, 594
HgaI GACGC 3 cut(s) 107, 356, 430
HhaI GCGC 4 cut(s) 92, 126, 171, 484
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 3 cut(s) 135, 190, 244
Hin6I GCGC 4 cut(s) 90, 124, 169, 482
HinP1I GCGC 4 cut(s) 90, 124, 169, 482
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HinfI GANTC 1 cut(s) 201
HpaII CCGG 2 cut(s) 226, 594
HphI GGTGA 2 cut(s) 406, 427
Hpy166II GTNNAC 2 cut(s) 63, 232
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 1 cut(s) 205
Hpy8I GTNNAC 2 cut(s) 63, 232
Hpy99I CGWCG 5 cut(s) 82, 320, 350, 383, 425
HpyAV CCTTC 1 cut(s) 536
HpyCH4IV ACGT 2 cut(s) 80, 453
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 107, 116, 148, 168, 404, 594
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 2 cut(s) 80, 453
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 3 cut(s) 135, 190, 244
HspAI GCGC 4 cut(s) 90, 124, 169, 482
Kzo9I GATC 2 cut(s) 372, 477
LmnI GCTCC 2 cut(s) 201, 355
Lsp1109I GCAGC 1 cut(s) 582
LweI GCATC 1 cut(s) 396
MaeI CTAG 1 cut(s) 601
MaeII ACGT 2 cut(s) 80, 453
MaeIII GTNAC 2 cut(s) 412, 492
MalI GATC 2 cut(s) 374, 479
MboI GATC 2 cut(s) 372, 477
MboII GAAGA 9 cut(s) 106, 244, 253, 292, 304, 319, 331, 355, 424
MfeI CAATTG 1 cut(s) 367
MlsI TGGCCA 1 cut(s) 191
MluCI AATT 2 cut(s) 13, 367
MluNI TGGCCA 1 cut(s) 191
Mox20I TGGCCA 1 cut(s) 191
MscI TGGCCA 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 191
MspI CCGG 2 cut(s) 226, 594
MspR9I CCNGG 2 cut(s) 227, 322
MunI CAATTG 1 cut(s) 367
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 6 cut(s) 59, 90, 124, 169, 345, 482
MwoI GCNNNNNNNGC 7 cut(s) 98, 107, 116, 148, 168, 404, 594
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 2 cut(s) 131, 240
NdeII GATC 2 cut(s) 372, 477
NlaIII CATG 3 cut(s) 135, 190, 244
NlaIV GGNNCC 2 cut(s) 37, 281
NmuCI GTSAC 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 137
PcsI WCGNNNNNNNCGW 6 cut(s) 243, 354, 387, 396, 417, 426
PfeI GAWTC 1 cut(s) 201
PflMI CCANNNNNTGG 1 cut(s) 444
PkrI GCNGC 6 cut(s) 25, 55, 58, 128, 502, 572
PpuMI RGGWCCY 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 2 cut(s) 37, 281
PspPI GGNCC 2 cut(s) 145, 535
PspPPI RGGWCCY 1 cut(s) 535
PspXI VCTCGAGB 1 cut(s) 137
PstNI CAGNNNCTG 1 cut(s) 444
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SatI GCNGC 6 cut(s) 24, 54, 57, 127, 501, 571
Sau3AI GATC 2 cut(s) 372, 477
Sau96I GGNCC 2 cut(s) 145, 535
ScrFI CCNGG 2 cut(s) 227, 322
SetI ASST 6 cut(s) 83, 144, 164, 216, 456, 540
SfaNI GCATC 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 137
SinI GGWCC 2 cut(s) 145, 535
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 2 cut(s) 13, 367
SsiI CCGC 9 cut(s) 24, 51, 54, 57, 127, 343, 385, 501, 546
SspMI CTAG 1 cut(s) 601
StyD4I CCNGG 2 cut(s) 225, 320
StyI CCWWGG 2 cut(s) 131, 240
TaiI ACGT 2 cut(s) 83, 456
TaqI TCGA 2 cut(s) 138, 378
TaqII GACCGA 1 cut(s) 550
TasI AATT 2 cut(s) 13, 367
TauI GCSGC 5 cut(s) 26, 56, 59, 129, 503
TfiI GAWTC 1 cut(s) 201
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 1 cut(s) 570
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 1 cut(s) 244
Van91I CCANNNNNTGG 1 cut(s) 444
VpaK11BI GGWCC 2 cut(s) 145, 535
XhoI CTCGAG 1 cut(s) 137
XspI CTAG 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.