MD05G1204600.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004291
Physical Location & Seq
Forward (+)
33420526 .. 33422535
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1204600.v1.1.491

Sequence Viewer

Length: 603 bp
ATGTCTTTAGATTCGCCACCCCGCTACGGCACACTCCCATCCGCCTCCTTCACCACCGCCACGCCCGAACCATACTCACGCGCCAGGGCCACGACCCAGTCCTTCTTCGCCACGCGCCGCCCATGGCTCGAGCTCGTTCAGCCCTTCTCTAGCTTCACGCGCCCCTACACCTTCGGCGAAGCCACCGTCCGAATCAAGCGCAACCTCGGCCACTTCCGCGTGAACTACACTATGCTTGTCCTCTTCGTCCTCTTCCTCAGCCTCCTCTGGCACCCCTTCTCCCTCATCGTCTTCCTCCTCGTCTTCGTCGCCTGGTTCTTCCTCTACTTCTTCCGCGACGAACCGCTCGTAATTTTCCACCGAATCGTCGACGACCGCGTCGTTTTGGGGATTCTCGCCGTCATCACCATCGTGGCTCTGGTCTTAACGCACGTGTGGCTGAACGTGCTGGTATCGGTTCTGATTGGGCTCGCGATTGTTGGGTTGCACGCGGCGTTTAGGGGGACGGATGATTTGTATGCCGACGAGCAGGAAGCTGCCGAAGGTGGGTTGCTTTCGGTTGTGGTGGGCAGTCCCAGGCGAACACGGTACACCCGCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

201

Amino Acids

22.74

Weight (kDa)

9.76

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 36 - 177 3.4e-50 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
AccB1I GGYRCC 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 369
AccII CGCG 9 cut(s) 81, 115, 160, 219, 336, 378, 473, 491, 597
AcoI YGGCCR 1 cut(s) 208
AcvI CACGTG 1 cut(s) 433
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 2 cut(s) 26, 546
AflIII ACRYGT 1 cut(s) 432
AjnI CCWGG 3 cut(s) 83, 311, 575
AleI CACNNNNGTG 1 cut(s) 410
AluBI AGCT 3 cut(s) 133, 153, 536
AluI AGCT 3 cut(s) 133, 153, 536
Alw21I GWGCWC 1 cut(s) 135
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 2 cut(s) 87, 208
ApeKI GCWGC 1 cut(s) 536
ArsI GACNNNNNNTTYG 4 cut(s) 171, 203, 496, 528
AspLEI GCGC 4 cut(s) 83, 117, 162, 201
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 2 cut(s) 43, 397
AvaI CYCGRG 1 cut(s) 128
BanI GGYRCC 1 cut(s) 270
BanII GRGCYC 2 cut(s) 135, 471
BbrPI CACGTG 1 cut(s) 433
BbsI GAAGAC 2 cut(s) 283, 295
Bbv12I GWGCWC 1 cut(s) 135
BbvCI CCTCAGC 1 cut(s) 257
BbvI GCAGC 1 cut(s) 523
BccI CCATC 2 cut(s) 46, 416
BceAI ACGGC 2 cut(s) 43, 383
BciT130I CCWGG 3 cut(s) 85, 313, 577
BfaI CTAG 1 cut(s) 150
BisI GCNGC 3 cut(s) 118, 492, 537
BlsI GCNGC 3 cut(s) 119, 493, 538
Bme1390I CCNGG 3 cut(s) 85, 313, 577
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 3 cut(s) 85, 313, 577
BmrI ACTGGG 1 cut(s) 91
BmuI ACTGGG 1 cut(s) 91
BpiI GAAGAC 2 cut(s) 283, 295
Bpu10I CCTNAGC 1 cut(s) 257
BsaAI YACGTR 1 cut(s) 433
BsaJI CCNNGG 4 cut(s) 84, 122, 205, 575
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 546
Bse1I ACTGG 1 cut(s) 97
BseBI CCWGG 3 cut(s) 85, 313, 577
BseDI CCNNGG 4 cut(s) 84, 122, 205, 575
BseGI GGATG 2 cut(s) 38, 514
BseLI CCNNNNNNNGG 2 cut(s) 26, 546
BseMII CTCAG 1 cut(s) 271
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 254, 287
BseXI GCAGC 1 cut(s) 523
Bsh1236I CGCG 9 cut(s) 81, 115, 160, 219, 336, 378, 473, 491, 597
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 2 cut(s) 89, 210
BshNI GGYRCC 1 cut(s) 270
BsiEI CGRYCG 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 128
BslFI GGGAC 2 cut(s) 517, 558
BslI CCNNNNNNNGG 2 cut(s) 26, 546
BsmFI GGGAC 2 cut(s) 517, 558
BsnI GGCC 2 cut(s) 89, 210
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 135, 471
Bsp19I CCATGG 1 cut(s) 122
Bsp68I TCGCGA 1 cut(s) 473
BspANI GGCC 2 cut(s) 89, 210
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 9 cut(s) 81, 115, 160, 219, 336, 378, 473, 491, 597
BspLI GGNNCC 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 270
BsrBI CCGCTC 1 cut(s) 346
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 4 cut(s) 84, 122, 205, 575
BssT1I CCWWGG 1 cut(s) 122
Bst2UI CCWGG 3 cut(s) 85, 313, 577
Bst4CI ACNGT 2 cut(s) 187, 588
Bst6I CTCTTC 2 cut(s) 248, 257
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 2 cut(s) 471, 489
BstDEI CTNAG 1 cut(s) 257
BstDSI CCRYGG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 38, 514
BstFNI CGCG 9 cut(s) 81, 115, 160, 219, 336, 378, 473, 491, 597
BstHHI GCGC 4 cut(s) 83, 117, 162, 201
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 6 cut(s) 139, 159, 207, 216, 436, 445
BstNI CCWGG 3 cut(s) 85, 313, 577
BstSCI CCNGG 3 cut(s) 83, 311, 575
BstUI CGCG 9 cut(s) 81, 115, 160, 219, 336, 378, 473, 491, 597
BstV1I GCAGC 1 cut(s) 523
BstV2I GAAGAC 2 cut(s) 283, 295
BsuRI GGCC 2 cut(s) 89, 210
BtgI CCRYGG 1 cut(s) 122
BtsCI GGATG 2 cut(s) 38, 514
BtuMI TCGCGA 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 471, 489
CfoI GCGC 4 cut(s) 83, 117, 162, 201
Cfr13I GGNCC 1 cut(s) 87
CseI GACGC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 589
CviAII CATG 1 cut(s) 123
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 1 cut(s) 257
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 2 cut(s) 248, 257
EarI CTCTTC 2 cut(s) 248, 257
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 122
Eco24I GRGCYC 2 cut(s) 135, 471
Eco53kI GAGCTC 1 cut(s) 133
Eco72I CACGTG 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 133
EcoRII CCWGG 3 cut(s) 83, 311, 575
EcoT14I CCWWGG 1 cut(s) 122
EcoT38I GRGCYC 2 cut(s) 135, 471
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 1 cut(s) 126
FaiI YATR 4 cut(s) 73, 124, 233, 519
FaqI GGGAC 2 cut(s) 517, 558
FatI CATG 1 cut(s) 122
FauI CCCGC 1 cut(s) 29
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 118, 492, 537
FokI GGATG 2 cut(s) 25, 521
FriOI GRGCYC 2 cut(s) 135, 471
Fsp4HI GCNGC 3 cut(s) 118, 492, 537
FspBI CTAG 1 cut(s) 150
GlaI GCGC 4 cut(s) 82, 116, 161, 200
GluI GCNGC 3 cut(s) 118, 492, 537
HaeIII GGCC 2 cut(s) 89, 210
HgaI GACGC 1 cut(s) 367
HhaI GCGC 4 cut(s) 83, 117, 162, 201
Hin1II CATG 1 cut(s) 126
Hin6I GCGC 4 cut(s) 81, 115, 160, 199
HinP1I GCGC 4 cut(s) 81, 115, 160, 199
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 4 cut(s) 11, 192, 363, 391
HphI GGTGA 2 cut(s) 43, 397
Hpy166II GTNNAC 3 cut(s) 223, 370, 591
Hpy188I TCNGA 2 cut(s) 191, 462
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 3 cut(s) 223, 370, 591
Hpy99I CGWCG 6 cut(s) 311, 341, 371, 374, 383, 527
HpyAV CCTTC 6 cut(s) 58, 112, 154, 181, 286, 536
HpyCH4III ACNGT 2 cut(s) 187, 588
HpyCH4IV ACGT 2 cut(s) 432, 444
HpyCH4V TGCA 1 cut(s) 487
HpyF10VI GCNNNNNNNGC 6 cut(s) 139, 159, 207, 216, 436, 445
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 2 cut(s) 432, 444
Hsp92II CATG 1 cut(s) 126
HspAI GCGC 4 cut(s) 81, 115, 160, 199
Lsp1109I GCAGC 1 cut(s) 523
MaeI CTAG 1 cut(s) 150
MaeII ACGT 2 cut(s) 432, 444
MbiI CCGCTC 1 cut(s) 346
MboII GAAGA 7 cut(s) 97, 235, 244, 283, 295, 310, 322
MhlI GDGCHC 2 cut(s) 135, 471
MluCI AATT 1 cut(s) 351
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 1 cut(s) 410
MspR9I CCNGG 3 cut(s) 85, 313, 577
MvaI CCWGG 3 cut(s) 85, 313, 577
MvnI CGCG 9 cut(s) 81, 115, 160, 219, 336, 378, 473, 491, 597
MwoI GCNNNNNNNGC 6 cut(s) 139, 159, 207, 216, 436, 445
NcoI CCATGG 1 cut(s) 122
NlaIII CATG 1 cut(s) 126
NlaIV GGNNCC 1 cut(s) 272
NmeAIII GCCGAG 1 cut(s) 186
NruI TCGCGA 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 128
PcsI WCGNNNNNNNCGW 3 cut(s) 345, 375, 378
PfeI GAWTC 4 cut(s) 11, 192, 363, 391
PflFI GACNNNGTC 2 cut(s) 97, 377
PkrI GCNGC 3 cut(s) 119, 493, 538
PmaCI CACGTG 1 cut(s) 433
PmlI CACGTG 1 cut(s) 433
Ppu21I YACGTR 1 cut(s) 433
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 3 cut(s) 83, 311, 575
PspCI CACGTG 1 cut(s) 433
PspGI CCWGG 3 cut(s) 83, 311, 575
PspN4I GGNNCC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 87
PspXI VCTCGAGB 1 cut(s) 128
PsyI GACNNNGTC 2 cut(s) 97, 377
RruI TCGCGA 1 cut(s) 473
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
RseI CAYNNNNRTG 1 cut(s) 410
SacI GAGCTC 1 cut(s) 135
SalI GTCGAC 1 cut(s) 368
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 3 cut(s) 118, 492, 537
Sau96I GGNCC 1 cut(s) 87
ScrFI CCNGG 3 cut(s) 85, 313, 577
SduI GDGCHC 2 cut(s) 135, 471
SetI ASST 8 cut(s) 135, 155, 173, 207, 435, 447, 538, 547
Sfr274I CTCGAG 1 cut(s) 128
SgrDI CGTCGACG 1 cut(s) 368
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 351
SspMI CTAG 1 cut(s) 150
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 83, 311, 575
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 187, 588
TaiI ACGT 2 cut(s) 435, 447
TaqI TCGA 2 cut(s) 129, 369
TasI AATT 1 cut(s) 351
TauI GCSGC 2 cut(s) 120, 494
TfiI GAWTC 4 cut(s) 11, 192, 363, 391
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TseI GCWGC 1 cut(s) 536
TspGWI ACGGA 1 cut(s) 521
Tth111I GACNNNGTC 2 cut(s) 97, 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.