MD10G1191700.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004291
Physical Location & Seq
Forward (+)
28835577 .. 28836740
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1191700.v1.1.491

Sequence Viewer

Length: 591 bp
ATGTCTTTAGATTCGCCACCGCACTACGGCACACATCCTTCCACTACCGCCTCGCCCGAACCCTTCTCACGCGCCAGAACCACGACCCAGTCCTTCTTCGCAACGCGCCGCCCATGGCTCGAGCTCGTCTATCCCCTCTCCAGCTTCACGCGCCCCTACACCTTCGGCGAAGCCACCGTCCGAATCAAGCGCAACCTCGGCCACTTCAGCGTAAACTACACGATGCTCGTCCTCTTTGTCCTCTTCCTCAGCCTCCTCTGGCACCCAGTCTCCCTCATTGTCTTCCTCCTCGTCTTCATCGCCTGGTTCTTCCTCTACTTCTTCCGCGACGAACCGCTCGTAATTTTCCACCGAATCGTCGACGACCGCGTCATTTTGGGGATTCTCGCCGTCGTCACCGTCGTGGCTCTGGTTTTCACGCACGTGTGGCTGAACGTGCTGGTGTCGATTCTGATTGGGGTCGCGATTGTTGGGTTGCACGCGGCGTTTAGGGGAACGGAGGATTTGTATGCCGACGAGCAGGAAGCTGCCGAAGGTGGGTTGCTCTCGGTTGTGGGCAGTCCCCCGCGGACTCGGTACAGCGGCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

197

Amino Acids

22.12

Weight (kDa)

7.07

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 33 - 174 1.6e-49 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 261
AccBSI CCGCTC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 360
AccII CGCG 8 cut(s) 72, 106, 151, 327, 369, 464, 482, 568
AcoI YGGCCR 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 190
AcvI CACGTG 1 cut(s) 424
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 2 cut(s) 26, 537
AflIII ACRYGT 1 cut(s) 423
AjnI CCWGG 1 cut(s) 302
AleI CACNNNNGTG 2 cut(s) 401, 422
AluBI AGCT 3 cut(s) 124, 144, 527
AluI AGCT 3 cut(s) 124, 144, 527
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 274
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 527
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspLEI GCGC 4 cut(s) 74, 108, 153, 192
AsuHPI GGTGA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 119
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 1 cut(s) 126
BbrPI CACGTG 1 cut(s) 424
BbsI GAAGAC 2 cut(s) 274, 286
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 514
BceAI ACGGC 2 cut(s) 43, 374
BciT130I CCWGG 1 cut(s) 304
BcoDI GTCTC 1 cut(s) 274
BisI GCNGC 4 cut(s) 109, 483, 528, 583
BlsI GCNGC 4 cut(s) 110, 484, 529, 584
Bme1390I CCNGG 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 304
BmrI ACTGGG 2 cut(s) 82, 260
BmsI GCATC 1 cut(s) 213
BmuI ACTGGG 2 cut(s) 82, 260
BpiI GAAGAC 2 cut(s) 274, 286
BpmI CTGGAG 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 424
BsaJI CCNNGG 3 cut(s) 113, 196, 566
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 491, 521
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 537
Bse1I ACTGG 2 cut(s) 88, 266
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 3 cut(s) 113, 196, 566
BseGI GGATG 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 26, 537
BseMII CTCAG 1 cut(s) 262
BseNI ACTGG 2 cut(s) 88, 266
BseRI GAGGAG 2 cut(s) 245, 278
BseXI GCAGC 1 cut(s) 514
Bsh1236I CGCG 8 cut(s) 72, 106, 151, 327, 369, 464, 482, 568
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 261
BsiEI CGRYCG 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 119
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 2 cut(s) 26, 537
BsmAI GTCTC 1 cut(s) 274
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 1 cut(s) 201
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 113
Bsp68I TCGCGA 1 cut(s) 464
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 261
BspFNI CGCG 8 cut(s) 72, 106, 151, 327, 369, 464, 482, 568
BspLI GGNNCC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 261
BsrBI CCGCTC 1 cut(s) 337
BsrI ACTGG 2 cut(s) 88, 266
BssECI CCNNGG 3 cut(s) 113, 196, 566
BssT1I CCWWGG 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 178, 400
Bst6I CTCTTC 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 1 cut(s) 248
BstDSI CCRYGG 2 cut(s) 113, 566
BstF5I GGATG 1 cut(s) 34
BstFNI CGCG 8 cut(s) 72, 106, 151, 327, 369, 464, 482, 568
BstHHI GCGC 4 cut(s) 74, 108, 153, 192
BstMAI GTCTC 1 cut(s) 274
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 5 cut(s) 150, 198, 207, 427, 436
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstUI CGCG 8 cut(s) 72, 106, 151, 327, 369, 464, 482, 568
BstV1I GCAGC 1 cut(s) 514
BstV2I GAAGAC 2 cut(s) 274, 286
BsuRI GGCC 1 cut(s) 201
BtgI CCRYGG 2 cut(s) 113, 566
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 1 cut(s) 34
BtuMI TCGCGA 1 cut(s) 464
Cac8I GCNNGC 1 cut(s) 480
CfoI GCGC 4 cut(s) 74, 108, 153, 192
Cfr42I CCGCGG 1 cut(s) 569
CseI GACGC 1 cut(s) 358
Csp6I GTAC 1 cut(s) 577
CviAII CATG 1 cut(s) 114
CviJI RGCY 9 cut(s) 118, 124, 144, 173, 201, 252, 407, 430, 527
CviKI_1 RGCY 9 cut(s) 118, 124, 144, 173, 201, 252, 407, 430, 527
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 1 cut(s) 248
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 248
EarI CTCTTC 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 190
Eco72I CACGTG 1 cut(s) 424
Eco88I CYCGRG 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 1 cut(s) 117
FaiI YATR 2 cut(s) 115, 510
FaqI GGGAC 1 cut(s) 546
FatI CATG 1 cut(s) 113
FauI CCCGC 1 cut(s) 573
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 4 cut(s) 109, 483, 528, 583
FokI GGATG 1 cut(s) 21
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 4 cut(s) 109, 483, 528, 583
GlaI GCGC 4 cut(s) 73, 107, 152, 191
GluI GCNGC 4 cut(s) 109, 483, 528, 583
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 1 cut(s) 201
HgaI GACGC 1 cut(s) 358
HhaI GCGC 4 cut(s) 74, 108, 153, 192
Hin1II CATG 1 cut(s) 117
Hin6I GCGC 4 cut(s) 72, 106, 151, 190
HinP1I GCGC 4 cut(s) 72, 106, 151, 190
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 6 cut(s) 11, 183, 354, 382, 448, 571
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 2 cut(s) 214, 361
Hpy188I TCNGA 2 cut(s) 182, 453
Hpy188III TCNNGA 1 cut(s) 463
Hpy8I GTNNAC 2 cut(s) 214, 361
Hpy99I CGWCG 6 cut(s) 332, 362, 365, 395, 404, 518
HpyAV CCTTC 5 cut(s) 48, 73, 103, 172, 527
HpyCH4III ACNGT 2 cut(s) 178, 400
HpyCH4IV ACGT 2 cut(s) 423, 435
HpyCH4V TGCA 1 cut(s) 478
HpyF10VI GCNNNNNNNGC 5 cut(s) 150, 198, 207, 427, 436
HpyF3I CTNAG 1 cut(s) 248
HpySE526I ACGT 2 cut(s) 423, 435
Hsp92II CATG 1 cut(s) 117
HspAI GCGC 4 cut(s) 72, 106, 151, 190
KspI CCGCGG 1 cut(s) 569
Lsp1109I GCAGC 1 cut(s) 514
LweI GCATC 1 cut(s) 213
MaeII ACGT 2 cut(s) 423, 435
MaeIII GTNAC 1 cut(s) 394
MbiI CCGCTC 1 cut(s) 337
MboII GAAGA 6 cut(s) 88, 235, 274, 286, 301, 313
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 1 cut(s) 342
MlyI GAGTC 1 cut(s) 565
MslI CAYNNNNRTG 2 cut(s) 401, 422
MspA1I CMGCKG 2 cut(s) 568, 582
MspR9I CCNGG 1 cut(s) 304
MvaI CCWGG 1 cut(s) 304
MvnI CGCG 8 cut(s) 72, 106, 151, 327, 369, 464, 482, 568
MwoI GCNNNNNNNGC 5 cut(s) 150, 198, 207, 427, 436
NcoI CCATGG 1 cut(s) 113
NlaIII CATG 1 cut(s) 117
NlaIV GGNNCC 1 cut(s) 263
NmeAIII GCCGAG 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 394
NruI TCGCGA 1 cut(s) 464
OliI CACNNNNGTG 2 cut(s) 401, 422
PaeR7I CTCGAG 1 cut(s) 119
PcsI WCGNNNNNNNCGW 3 cut(s) 336, 366, 399
PfeI GAWTC 5 cut(s) 11, 183, 354, 382, 448
PflFI GACNNNGTC 2 cut(s) 88, 368
PkrI GCNGC 4 cut(s) 110, 484, 529, 584
PleI GAGTC 1 cut(s) 565
PmaCI CACGTG 1 cut(s) 424
PmlI CACGTG 1 cut(s) 424
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 302
PspCI CACGTG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 263
PspXI VCTCGAGB 1 cut(s) 119
PsyI GACNNNGTC 2 cut(s) 88, 368
RruI TCGCGA 1 cut(s) 464
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
RseI CAYNNNNRTG 2 cut(s) 401, 422
SacI GAGCTC 1 cut(s) 126
SacII CCGCGG 1 cut(s) 569
SalI GTCGAC 1 cut(s) 359
SatI GCNGC 4 cut(s) 109, 483, 528, 583
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 1 cut(s) 304
SduI GDGCHC 1 cut(s) 126
SetI ASST 8 cut(s) 126, 146, 164, 198, 426, 438, 529, 538
SfaNI GCATC 1 cut(s) 213
Sfr274I CTCGAG 1 cut(s) 119
Sfr303I CCGCGG 1 cut(s) 569
SgrBI CCGCGG 1 cut(s) 569
SgrDI CGTCGACG 1 cut(s) 359
SlaI CTCGAG 1 cut(s) 119
SmiMI CAYNNNNRTG 2 cut(s) 401, 422
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 342
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 113
TaaI ACNGT 2 cut(s) 178, 400
TaiI ACGT 2 cut(s) 426, 438
TaqI TCGA 3 cut(s) 120, 360, 446
TasI AATT 1 cut(s) 342
TauI GCSGC 3 cut(s) 111, 485, 585
TfiI GAWTC 5 cut(s) 11, 183, 354, 382, 448
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 1 cut(s) 527
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 1 cut(s) 286
TspGWI ACGGA 1 cut(s) 512
Tth111I GACNNNGTC 2 cut(s) 88, 368
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.