Rroxscaffold_6G00428320

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004291
Physical Location & Seq
Forward (+)
48766817 .. 48767407
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428320.1

Sequence Viewer

Length: 591 bp
ATGTCGCTAAACCCACCCGCGGGTTACGGCACCATCACCGGCGGTGAAACCTCGACCAAAGACGTCACCTTTGACTCGACCTCCCAAACCGTGTACCCCACGCGCCGCCCATGGTCCGAGCTTTTCTCCCCCACCGCCTTCTCCGTCCCCTACAGCTACGCCGACGCCATGAGCCGCGTGAAGCACAACCTCAGCTACTTCCGTCTCAACTACGCCATGGTCGTGCTCTTCATCGTCTTCCTCAGCCTCCTATGGCACCCCGTCTCCATGATCGTCTTCTTGATCATCCTCATCTACTGGATCTTCGGTTACTTCTCACGCGCCCCCGGCCCCGTCGTCCTCTTCAACCGGACCTTCGACGACGGCGCCGTCCTCTTCGCCCTCAGCCTCCTCACCGTGGTGGCGCTTGTGCTCACGCGCGTGGGGCTGAACGTGCTGGTCTCTCTGGTCGTCGCCGTGGTCGTTGTGGGCCTCCACGCCGCGTTTCGGGTCACAGAAGACCTGTATTCCGATCCGGAGAGTGCTGCTGATGGTGGGTTGGTTTCCGTTGTTGGATCGGAGCCGTCTCGCCCGGCCCACACCCGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

196

Amino Acids

21.51

Weight (kDa)

6.4

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 32 - 171 6.4e-47 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AatII GACGTC 1 cut(s) 66
AccB1I GGYRCC 3 cut(s) 29, 255, 365
AccII CGCG 7 cut(s) 20, 103, 177, 321, 418, 420, 482
AccIII TCCGGA 1 cut(s) 514
AciI CCGC 7 cut(s) 18, 20, 42, 106, 135, 175, 480
AclWI GGATC 3 cut(s) 308, 506, 562
AcyI GRCGYC 3 cut(s) 63, 165, 366
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 5 cut(s) 19, 20, 397, 486, 582
AgsI TTSAA 1 cut(s) 346
AleI CACNNNNGTG 2 cut(s) 398, 419
AluBI AGCT 3 cut(s) 121, 156, 195
AluI AGCT 3 cut(s) 121, 156, 195
Alw21I GWGCWC 2 cut(s) 228, 414
Alw26I GTCTC 4 cut(s) 209, 268, 445, 570
AlwI GGATC 3 cut(s) 308, 506, 562
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 3 cut(s) 328, 469, 573
ApeKI GCWGC 1 cut(s) 524
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 5 cut(s) 105, 323, 368, 406, 420
AspS9I GGNCC 5 cut(s) 114, 329, 351, 469, 574
AsuC2I CCSGG 3 cut(s) 327, 572, 583
AsuHPI GGTGA 4 cut(s) 28, 56, 58, 385
AvaII GGWCC 2 cut(s) 114, 351
BanI GGYRCC 3 cut(s) 29, 255, 365
BbsI GAAGAC 3 cut(s) 229, 268, 504
Bbv12I GWGCWC 2 cut(s) 228, 414
BbvCI CCTCAGC 3 cut(s) 191, 242, 383
BbvI GCAGC 1 cut(s) 511
BccI CCATC 2 cut(s) 41, 524
BceAI ACGGC 5 cut(s) 43, 353, 379, 440, 547
BclI TGATCA 1 cut(s) 282
BcnI CCSGG 3 cut(s) 327, 572, 583
BcoDI GTCTC 4 cut(s) 209, 268, 445, 570
BfmI CTRYAG 1 cut(s) 151
BfoI RGCGCY 2 cut(s) 369, 407
BisI GCNGC 4 cut(s) 106, 175, 480, 525
BlsI GCNGC 4 cut(s) 107, 176, 481, 526
Bme1390I CCNGG 3 cut(s) 327, 572, 583
Bme18I GGWCC 2 cut(s) 114, 351
BmgT120I GGNCC 5 cut(s) 114, 329, 351, 469, 574
BmiI GGNNCC 5 cut(s) 31, 257, 331, 367, 561
BmrFI CCNGG 3 cut(s) 327, 572, 583
BpiI GAAGAC 3 cut(s) 229, 268, 504
BplI GAGNNNNNCTC 2 cut(s) 110, 142
Bpu10I CCTNAGC 3 cut(s) 191, 242, 383
BpuMI CCSGG 3 cut(s) 327, 572, 583
BsaHI GRCGYC 3 cut(s) 63, 165, 366
BsaI GGTCTC 1 cut(s) 445
BsaJI CCNNGG 6 cut(s) 18, 110, 216, 325, 396, 456
BsaWI WCCGGW 2 cut(s) 348, 514
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 248, 278
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 20, 397, 486, 582
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 302
BseAI TCCGGA 1 cut(s) 514
BseDI CCNNGG 6 cut(s) 18, 110, 216, 325, 396, 456
BseGI GGATG 1 cut(s) 285
BseLI CCNNNNNNNGG 5 cut(s) 19, 20, 397, 486, 582
BseMII CTCAG 3 cut(s) 205, 256, 397
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 1 cut(s) 511
Bsh1236I CGCG 7 cut(s) 20, 103, 177, 321, 418, 420, 482
BshFI GGCC 3 cut(s) 330, 471, 575
BshNI GGYRCC 3 cut(s) 29, 255, 365
BsiHKAI GWGCWC 2 cut(s) 228, 414
BsiSI CCGG 6 cut(s) 39, 327, 349, 515, 572, 583
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 5 cut(s) 19, 20, 397, 486, 582
BsmAI GTCTC 4 cut(s) 209, 268, 445, 570
BsmBI CGTCTC 3 cut(s) 209, 268, 570
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 3 cut(s) 330, 471, 575
Bso31I GGTCTC 1 cut(s) 445
Bsp1286I GDGCHC 2 cut(s) 228, 414
Bsp13I TCCGGA 1 cut(s) 514
Bsp143I GATC 5 cut(s) 270, 282, 300, 511, 554
Bsp19I CCATGG 2 cut(s) 110, 216
BspACI CCGC 7 cut(s) 18, 20, 42, 106, 135, 175, 480
BspANI GGCC 3 cut(s) 330, 471, 575
BspCNI CTCAG 3 cut(s) 204, 255, 396
BspEI TCCGGA 1 cut(s) 514
BspFNI CGCG 7 cut(s) 20, 103, 177, 321, 418, 420, 482
BspLI GGNNCC 5 cut(s) 31, 257, 331, 367, 561
BspPI GGATC 3 cut(s) 308, 506, 562
BspQI GCTCTTC 1 cut(s) 233
BspT107I GGYRCC 3 cut(s) 29, 255, 365
BspTNI GGTCTC 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 6 cut(s) 18, 110, 216, 325, 396, 456
BssMI GATC 5 cut(s) 270, 282, 300, 511, 554
BssNI GRCGYC 3 cut(s) 63, 165, 366
BssT1I CCWWGG 2 cut(s) 110, 216
Bst4CI ACNGT 2 cut(s) 91, 397
Bst6I CTCTTC 3 cut(s) 233, 347, 380
BstACI GRCGYC 3 cut(s) 63, 165, 366
BstDEI CTNAG 3 cut(s) 191, 242, 383
BstDSI CCRYGG 5 cut(s) 18, 110, 216, 396, 456
BstF5I GGATG 1 cut(s) 285
BstFNI CGCG 7 cut(s) 20, 103, 177, 321, 418, 420, 482
BstH2I RGCGCY 2 cut(s) 369, 407
BstHHI GCGC 5 cut(s) 105, 323, 368, 406, 420
BstKTI GATC 5 cut(s) 273, 285, 303, 514, 557
BstMAI GTCTC 4 cut(s) 209, 268, 445, 570
BstMBI GATC 5 cut(s) 270, 282, 300, 511, 554
BstMWI GCNNNNNNNGC 3 cut(s) 327, 424, 433
BstSCI CCNGG 3 cut(s) 325, 570, 581
BstSFI CTRYAG 1 cut(s) 151
BstUI CGCG 7 cut(s) 20, 103, 177, 321, 418, 420, 482
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 3 cut(s) 229, 268, 504
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BsuRI GGCC 3 cut(s) 330, 471, 575
BtgI CCRYGG 5 cut(s) 18, 110, 216, 396, 456
BtsCI GGATG 1 cut(s) 285
CfoI GCGC 5 cut(s) 105, 323, 368, 406, 420
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 5 cut(s) 114, 329, 351, 469, 574
Cfr42I CCGCGG 1 cut(s) 21
CseI GACGC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 94
CviAII CATG 4 cut(s) 111, 169, 217, 268
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 3 cut(s) 191, 242, 383
DinI GGCGCC 1 cut(s) 367
DpnI GATC 5 cut(s) 272, 284, 302, 513, 556
DpnII GATC 5 cut(s) 270, 282, 300, 511, 554
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 3 cut(s) 233, 347, 380
EarI CTCTTC 3 cut(s) 233, 347, 380
Eco130I CCWWGG 2 cut(s) 110, 216
Eco31I GGTCTC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 114, 351
EcoT14I CCWWGG 2 cut(s) 110, 216
EgeI GGCGCC 1 cut(s) 367
EheI GGCGCC 1 cut(s) 367
ErhI CCWWGG 2 cut(s) 110, 216
Esp3I CGTCTC 3 cut(s) 209, 268, 570
FaeI CATG 4 cut(s) 114, 172, 220, 271
FaiI YATR 5 cut(s) 112, 170, 218, 253, 269
FaqI GGGAC 1 cut(s) 131
FatI CATG 4 cut(s) 110, 168, 216, 267
FauI CCCGC 2 cut(s) 13, 25
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 4 cut(s) 106, 175, 480, 525
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 4 cut(s) 106, 175, 480, 525
GlaI GCGC 5 cut(s) 104, 322, 367, 405, 419
GluI GCNGC 4 cut(s) 106, 175, 480, 525
HaeII RGCGCY 2 cut(s) 369, 407
HaeIII GGCC 3 cut(s) 330, 471, 575
HapII CCGG 6 cut(s) 39, 327, 349, 515, 572, 583
HgaI GACGC 1 cut(s) 173
HhaI GCGC 5 cut(s) 105, 323, 368, 406, 420
Hin1I GRCGYC 3 cut(s) 63, 165, 366
Hin1II CATG 4 cut(s) 114, 172, 220, 271
Hin6I GCGC 5 cut(s) 103, 321, 366, 404, 418
HinP1I GCGC 5 cut(s) 103, 321, 366, 404, 418
HinfI GANTC 1 cut(s) 74
HpaII CCGG 6 cut(s) 39, 327, 349, 515, 572, 583
HphI GGTGA 4 cut(s) 28, 56, 58, 385
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 118, 511, 559
Hpy188III TCNNGA 2 cut(s) 280, 515
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 5 cut(s) 167, 338, 362, 365, 455
HpyAV CCTTC 2 cut(s) 148, 364
HpyCH4III ACNGT 2 cut(s) 91, 397
HpyCH4IV ACGT 2 cut(s) 63, 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 424, 433
HpyF3I CTNAG 3 cut(s) 191, 242, 383
HpySE526I ACGT 2 cut(s) 63, 432
Hsp92I GRCGYC 3 cut(s) 63, 165, 366
Hsp92II CATG 4 cut(s) 114, 172, 220, 271
HspAI GCGC 5 cut(s) 103, 321, 366, 404, 418
KasI GGCGCC 1 cut(s) 365
Kpn2I TCCGGA 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 282
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 5 cut(s) 270, 282, 300, 511, 554
LguI GCTCTTC 1 cut(s) 233
LmnI GCTCC 1 cut(s) 559
LpnPI CCDG 9 cut(s) 52, 283, 340, 362, 422, 431, 515, 528, 585
Lsp1109I GCAGC 1 cut(s) 511
MaeII ACGT 2 cut(s) 63, 432
MaeIII GTNAC 4 cut(s) 23, 64, 308, 490
MalI GATC 5 cut(s) 272, 284, 302, 513, 556
MboI GATC 5 cut(s) 270, 282, 300, 511, 554
MboII GAAGA 7 cut(s) 220, 229, 268, 295, 334, 367, 509
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 2 cut(s) 228, 414
Mly113I GGCGCC 1 cut(s) 366
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 532
MroI TCCGGA 1 cut(s) 514
MslI CAYNNNNRTG 3 cut(s) 221, 398, 419
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 6 cut(s) 39, 327, 349, 515, 572, 583
MspR9I CCNGG 3 cut(s) 327, 572, 583
MvnI CGCG 7 cut(s) 20, 103, 177, 321, 418, 420, 482
MwoI GCNNNNNNNGC 3 cut(s) 327, 424, 433
NarI GGCGCC 1 cut(s) 366
NciI CCSGG 3 cut(s) 327, 572, 583
NcoI CCATGG 2 cut(s) 110, 216
NdeII GATC 5 cut(s) 270, 282, 300, 511, 554
NlaIII CATG 4 cut(s) 114, 172, 220, 271
NlaIV GGNNCC 5 cut(s) 31, 257, 331, 367, 561
NmuCI GTSAC 2 cut(s) 64, 490
OliI CACNNNNGTG 2 cut(s) 398, 419
PciSI GCTCTTC 1 cut(s) 233
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 366, 459
PkrI GCNGC 4 cut(s) 107, 176, 481, 526
PleI GAGTC 1 cut(s) 68
PluTI GGCGCC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 68
PspN4I GGNNCC 5 cut(s) 31, 257, 331, 367, 561
PspPI GGNCC 5 cut(s) 114, 329, 351, 469, 574
PsuI RGATCY 1 cut(s) 300
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 3 cut(s) 221, 398, 419
SacII CCGCGG 1 cut(s) 21
SapI GCTCTTC 1 cut(s) 233
SatI GCNGC 4 cut(s) 106, 175, 480, 525
Sau3AI GATC 5 cut(s) 270, 282, 300, 511, 554
Sau96I GGNCC 5 cut(s) 114, 329, 351, 469, 574
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 3 cut(s) 327, 572, 583
SduI GDGCHC 2 cut(s) 228, 414
SfcI CTRYAG 1 cut(s) 151
SfoI GGCGCC 1 cut(s) 367
Sfr303I CCGCGG 1 cut(s) 21
SgrAI CRCCGGYG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 2 cut(s) 114, 351
SmiMI CAYNNNNRTG 3 cut(s) 221, 398, 419
SsiI CCGC 7 cut(s) 18, 20, 42, 106, 135, 175, 480
SspDI GGCGCC 1 cut(s) 365
StyD4I CCNGG 3 cut(s) 325, 570, 581
StyI CCWWGG 2 cut(s) 110, 216
TaaI ACNGT 2 cut(s) 91, 397
TaiI ACGT 2 cut(s) 66, 435
TaqI TCGA 3 cut(s) 53, 77, 357
TauI GCSGC 3 cut(s) 108, 177, 482
TseFI GTSAC 2 cut(s) 64, 490
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 2 cut(s) 64, 490
TspDTI ATGAA 1 cut(s) 220
TspGWI ACGGA 3 cut(s) 133, 191, 535
VpaK11BI GGWCC 2 cut(s) 114, 351
ZraI GACGTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.