pycom04g19910

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004291
Physical Location & Seq
Reverse (-)
21520591 .. 21521095
505 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19910.1

Sequence Viewer

Length: 459 bp
ATGCCGCTAAAAACACCGGCGGGTTACGGCGGCACCACCGTCACCGCCGCCCAAATCGACCCGCAAACCTCCAAATACCTCACCTTCACCTCCCGCCCTGTACCCCAGACCTCCTCCCAACCCCTGTACCCCACTCTGAACTCCCAAACCGTGTACCCGACGAGTCGCCCATGGCCCGAGATCTTCTCCCTCACCTCCTTCTCCGTACCCTACAACTACGCCGACGCAATTGCACGAAGCAAGCGCAACACCGCCTACTTCCGCGTCAACTACGTCATGGCGACGCTCTTCATCGTCTTCTGCAGCCTCCTCTGGCACCCGATCTCTATGATCATCTTCCTCATCGTCCTCGTCGCCTGGCTCGCCCTCTACTTCTTCCGCACCACCCCCGTTGTTCTGTTCAATCAGAGCTTCGACGACAGGATCGTCGCCGTCGTGCTGGGATTGGTCACTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

153

Amino Acids

17.17

Weight (kDa)

9.56

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 53 - 151 6.8e-31 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AccB1I GGYRCC 2 cut(s) 32, 315
AccII CGCG 1 cut(s) 264
AclWI GGATC 1 cut(s) 431
AfaI GTAC 4 cut(s) 102, 128, 155, 207
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 1 cut(s) 411
AluI AGCT 1 cut(s) 411
AlwI GGATC 1 cut(s) 431
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 303
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 174
AsuHPI GGTGA 4 cut(s) 34, 73, 79, 184
AvaI CYCGRG 1 cut(s) 176
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BanI GGYRCC 2 cut(s) 32, 315
BbsI GAAGAC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 315
BceAI ACGGC 2 cut(s) 43, 416
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 1 cut(s) 358
BclI TGATCA 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 301
BglII AGATCT 1 cut(s) 180
BisI GCNGC 4 cut(s) 5, 31, 48, 304
BlsI GCNGC 4 cut(s) 6, 32, 49, 305
Bme1390I CCNGG 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 34, 317
BmrFI CCNGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 289
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BsaJI CCNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 16
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 1 cut(s) 170
BseRI GAGGAG 2 cut(s) 103, 299
BseXI GCAGC 1 cut(s) 315
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 1 cut(s) 175
BshNI GGYRCC 2 cut(s) 32, 315
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 17
BsnI GGCC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 176
Bsp143I GATC 4 cut(s) 180, 321, 330, 423
Bsp19I CCATGG 1 cut(s) 170
BspANI GGCC 1 cut(s) 175
BspFNI CGCG 1 cut(s) 264
BspLI GGNNCC 2 cut(s) 34, 317
BspMAI CTGCAG 1 cut(s) 305
BspPI GGATC 1 cut(s) 431
BspQI GCTCTTC 1 cut(s) 293
BspT107I GGYRCC 2 cut(s) 32, 315
BsrFI RCCGGY 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 4 cut(s) 180, 321, 330, 423
BssT1I CCWWGG 1 cut(s) 170
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 40, 151
Bst6I CTCTTC 1 cut(s) 293
BstC8I GCNNGC 2 cut(s) 242, 363
BstDSI CCRYGG 1 cut(s) 170
BstFNI CGCG 1 cut(s) 264
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 4 cut(s) 183, 324, 333, 426
BstMBI GATC 4 cut(s) 180, 321, 330, 423
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 301
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 315
BstV2I GAAGAC 1 cut(s) 289
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 1 cut(s) 175
BtgI CCRYGG 1 cut(s) 170
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 2 cut(s) 242, 363
CfoI GCGC 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 174
CseI GACGC 3 cut(s) 233, 253, 292
Csp6I GTAC 4 cut(s) 101, 127, 154, 206
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviAII CATG 2 cut(s) 171, 277
CviJI RGCY 4 cut(s) 175, 306, 361, 411
CviKI_1 RGCY 4 cut(s) 175, 306, 361, 411
CviQI GTAC 4 cut(s) 101, 127, 154, 206
DpnI GATC 4 cut(s) 182, 323, 332, 425
DpnII GATC 4 cut(s) 180, 321, 330, 423
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 2 cut(s) 174, 280
FaiI YATR 3 cut(s) 172, 278, 329
FatI CATG 2 cut(s) 170, 276
FauI CCCGC 3 cut(s) 13, 69, 101
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 5, 31, 48, 304
Fsp4HI GCNGC 4 cut(s) 5, 31, 48, 304
GlaI GCGC 1 cut(s) 245
GluI GCNGC 4 cut(s) 5, 31, 48, 304
GsaI CCCAGC 1 cut(s) 443
HaeIII GGCC 1 cut(s) 175
HapII CCGG 1 cut(s) 17
HgaI GACGC 3 cut(s) 233, 253, 292
HhaI GCGC 1 cut(s) 246
Hin1II CATG 2 cut(s) 174, 280
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 1 cut(s) 163
HpaII CCGG 1 cut(s) 17
HphI GGTGA 4 cut(s) 34, 73, 79, 184
Hpy166II GTNNAC 2 cut(s) 154, 268
Hpy188I TCNGA 2 cut(s) 138, 408
Hpy8I GTNNAC 2 cut(s) 154, 268
Hpy99I CGWCG 7 cut(s) 163, 227, 286, 356, 419, 431, 437
HpyAV CCTTC 2 cut(s) 94, 208
HpyCH4III ACNGT 2 cut(s) 40, 151
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 233, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 2 cut(s) 174, 280
HspAI GCGC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 4 cut(s) 180, 321, 330, 423
LguI GCTCTTC 1 cut(s) 293
LpnPI CCDG 9 cut(s) 30, 111, 119, 137, 298, 343, 370, 406, 425
Lsp1109I GCAGC 1 cut(s) 315
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 3 cut(s) 23, 40, 448
MalI GATC 4 cut(s) 182, 323, 332, 425
MboI GATC 4 cut(s) 180, 321, 330, 423
MboII GAAGA 5 cut(s) 175, 280, 289, 328, 367
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 180
MluCI AATT 1 cut(s) 228
MlyI GAGTC 1 cut(s) 172
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 358
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 1 cut(s) 362
NcoI CCATGG 1 cut(s) 170
NdeII GATC 4 cut(s) 180, 321, 330, 423
NlaIII CATG 2 cut(s) 174, 280
NlaIV GGNNCC 2 cut(s) 34, 317
NmuCI GTSAC 2 cut(s) 40, 448
PciSI GCTCTTC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 2 cut(s) 423, 432
PkrI GCNGC 4 cut(s) 6, 32, 49, 305
PleI GAGTC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 356
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 2 cut(s) 34, 317
PspPI GGNCC 1 cut(s) 174
PstI CTGCAG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 4 cut(s) 102, 128, 155, 207
RsaNI GTAC 4 cut(s) 101, 127, 154, 206
SapI GCTCTTC 1 cut(s) 293
SatI GCNGC 4 cut(s) 5, 31, 48, 304
Sau3AI GATC 4 cut(s) 180, 321, 330, 423
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 1 cut(s) 358
SetI ASST 8 cut(s) 71, 81, 86, 92, 113, 197, 276, 413
SfcI CTRYAG 1 cut(s) 301
SgrAI CRCCGGYG 1 cut(s) 16
Sse9I AATT 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 356
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 2 cut(s) 40, 151
TaiI ACGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 57, 414
TasI AATT 1 cut(s) 228
TauI GCSGC 3 cut(s) 7, 33, 50
TseFI GTSAC 2 cut(s) 40, 448
TseI GCWGC 1 cut(s) 303
Tsp45I GTSAC 2 cut(s) 40, 448
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.