MD12G1243000.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004291
Physical Location & Seq
Reverse (-)
31389535 .. 31390191
657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1243000.v1.1.491

Sequence Viewer

Length: 657 bp
ATGCCGCTAAAACCACAGTCGAGTTACGGCACCACCACCATCACCGCCGCCCAAATCGACCCCCAAACGACCAAAGACCTCACCTTTACCTCCCGCCCCAAACCCCAGACCTCCTTCCAACCCCTGTACCCCACTATCACCTCCCAATCCGTGTACCCGACGCGCCGCCCATGGCCCGAGCTCTTTTCTCTCACCTCCTTCTCCGTCCCATCCAACTACGCCGACGCCATGGCGCGAATCAAGCTCAACACCGCCTACTTCCGCGTCAACTACACCATGGCGGCGCTCTTCATCGTCTTCCTCAGCCTCCTCTGGCACCCGATCTCCATGATCGTCTTCCTCATCGTCCTCGTCGCCTGGCTCGCCCTCTACTTCTTCCGCACCACCCCCGTCGCTCTGTACAACCAGAGCCTCGACGACAGGGTCGTTGCCATCGCGCTGGGATTGGTCACTGTCGTGGCGCTGGTATTCACTCACGTGGGTTTGAATGTGCTGGTGGCGCTGATCGTCGCCGTCGTGATTATGGGGCTCCACGCGGCGTTTCGAGTCACGGAAGATTTGTTTCTCGACGAGGAGACCGCCGCGGAAAACGGGTTGGCCTCGGTTGTTGCCAGCCAGCAGTCCCTACGCCTGCCGACTTCTTACACCCGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

219

Amino Acids

24.21

Weight (kDa)

8.92

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 52 - 192 1.8e-46 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AccB1I GGYRCC 2 cut(s) 29, 315
AccII CGCG 6 cut(s) 163, 235, 264, 437, 536, 584
AcvI CACGTG 1 cut(s) 478
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 3 cut(s) 128, 155, 401
AgsI TTSAA 1 cut(s) 487
AjnI CCWGG 1 cut(s) 356
AleI CACNNNNGTG 2 cut(s) 455, 476
AluBI AGCT 2 cut(s) 181, 244
AluI AGCT 2 cut(s) 181, 244
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 2 cut(s) 173, 597
AspLEI GCGC 6 cut(s) 165, 235, 286, 439, 463, 502
AspS9I GGNCC 1 cut(s) 174
AsuC2I CCSGG 1 cut(s) 649
AsuHPI GGTGA 4 cut(s) 34, 73, 130, 184
AvaI CYCGRG 1 cut(s) 176
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BanI GGYRCC 2 cut(s) 29, 315
BanII GRGCYC 2 cut(s) 183, 531
BbrPI CACGTG 1 cut(s) 478
BbsI GAAGAC 2 cut(s) 289, 328
Bbv12I GWGCWC 1 cut(s) 183
BbvCI CCTCAGC 1 cut(s) 302
BccI CCATC 3 cut(s) 47, 217, 440
BceAI ACGGC 2 cut(s) 43, 497
BciT130I CCWGG 1 cut(s) 358
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 1 cut(s) 569
BfoI RGCGCY 3 cut(s) 287, 464, 503
BisI GCNGC 6 cut(s) 5, 48, 166, 282, 537, 582
BlsI GCNGC 6 cut(s) 6, 49, 167, 283, 538, 583
Bme1390I CCNGG 2 cut(s) 358, 649
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 3 cut(s) 31, 317, 530
BmrFI CCNGG 2 cut(s) 358, 649
BpiI GAAGAC 2 cut(s) 289, 328
Bpu10I CCTNAGC 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 649
BsaAI YACGTR 1 cut(s) 478
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 5 cut(s) 170, 228, 276, 582, 600
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 5 cut(s) 170, 228, 276, 582, 600
BseGI GGATG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 316
BseRI GAGGAG 2 cut(s) 299, 587
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 6 cut(s) 163, 235, 264, 437, 536, 584
BshFI GGCC 2 cut(s) 175, 599
BshNI GGYRCC 2 cut(s) 29, 315
BsiHKAI GWGCWC 1 cut(s) 183
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 649
BslFI GGGAC 2 cut(s) 191, 607
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 2 cut(s) 191, 607
BsnI GGCC 2 cut(s) 175, 599
Bso31I GGTCTC 1 cut(s) 569
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 183, 531
Bsp1407I TGTACA 1 cut(s) 399
Bsp143I GATC 3 cut(s) 321, 330, 504
Bsp19I CCATGG 3 cut(s) 170, 228, 276
BspANI GGCC 2 cut(s) 175, 599
BspCNI CTCAG 1 cut(s) 315
BspFNI CGCG 6 cut(s) 163, 235, 264, 437, 536, 584
BspLI GGNNCC 3 cut(s) 31, 317, 530
BspQI GCTCTTC 1 cut(s) 293
BspT107I GGYRCC 2 cut(s) 29, 315
BspTNI GGTCTC 1 cut(s) 569
BsrGI TGTACA 1 cut(s) 399
BssECI CCNNGG 5 cut(s) 170, 228, 276, 582, 600
BssMI GATC 3 cut(s) 321, 330, 504
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 3 cut(s) 170, 228, 276
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 18, 454
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 225
BstAUI TGTACA 1 cut(s) 399
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 4 cut(s) 363, 613, 617, 632
BstDEI CTNAG 1 cut(s) 302
BstDSI CCRYGG 4 cut(s) 170, 228, 276, 582
BstF5I GGATG 1 cut(s) 209
BstFNI CGCG 6 cut(s) 163, 235, 264, 437, 536, 584
BstH2I RGCGCY 3 cut(s) 287, 464, 503
BstHHI GCGC 6 cut(s) 165, 235, 286, 439, 463, 502
BstKTI GATC 3 cut(s) 324, 333, 507
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 3 cut(s) 321, 330, 504
BstMWI GCNNNNNNNGC 3 cut(s) 241, 362, 499
BstNI CCWGG 1 cut(s) 358
BstSCI CCNGG 2 cut(s) 356, 647
BstUI CGCG 6 cut(s) 163, 235, 264, 437, 536, 584
BstV2I GAAGAC 2 cut(s) 289, 328
BstXI CCANNNNNNTGG 1 cut(s) 439
BsuRI GGCC 2 cut(s) 175, 599
BtgI CCRYGG 4 cut(s) 170, 228, 276, 582
BtgZI GCGATG 1 cut(s) 418
BtsCI GGATG 1 cut(s) 209
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 4 cut(s) 363, 613, 617, 632
CfoI GCGC 6 cut(s) 165, 235, 286, 439, 463, 502
Cfr13I GGNCC 1 cut(s) 174
Cfr42I CCGCGG 1 cut(s) 585
CseI GACGC 3 cut(s) 169, 233, 253
Csp6I GTAC 3 cut(s) 127, 154, 400
CviAII CATG 4 cut(s) 171, 229, 277, 328
CviJI RGCY 9 cut(s) 175, 181, 244, 306, 361, 411, 529, 599, 615
CviKI_1 RGCY 9 cut(s) 175, 181, 244, 306, 361, 411, 529, 599, 615
CviQI GTAC 3 cut(s) 127, 154, 400
DdeI CTNAG 1 cut(s) 302
DpnI GATC 3 cut(s) 323, 332, 506
DpnII GATC 3 cut(s) 321, 330, 504
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 3 cut(s) 170, 228, 276
Eco24I GRGCYC 2 cut(s) 183, 531
Eco31I GGTCTC 1 cut(s) 569
Eco53kI GAGCTC 1 cut(s) 181
Eco72I CACGTG 1 cut(s) 478
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 3 cut(s) 170, 228, 276
EcoT38I GRGCYC 2 cut(s) 183, 531
ErhI CCWWGG 3 cut(s) 170, 228, 276
FaeI CATG 4 cut(s) 174, 232, 280, 331
FaiI YATR 5 cut(s) 172, 230, 278, 329, 524
FaqI GGGAC 2 cut(s) 191, 607
FatI CATG 4 cut(s) 170, 228, 276, 327
FauI CCCGC 1 cut(s) 101
Fnu4HI GCNGC 6 cut(s) 5, 48, 166, 282, 537, 582
FokI GGATG 1 cut(s) 196
FriOI GRGCYC 2 cut(s) 183, 531
Fsp4HI GCNGC 6 cut(s) 5, 48, 166, 282, 537, 582
GlaI GCGC 6 cut(s) 164, 234, 285, 438, 462, 501
GluI GCNGC 6 cut(s) 5, 48, 166, 282, 537, 582
GsaI CCCAGC 1 cut(s) 443
HaeII RGCGCY 3 cut(s) 287, 464, 503
HaeIII GGCC 2 cut(s) 175, 599
HapII CCGG 1 cut(s) 649
HgaI GACGC 3 cut(s) 169, 233, 253
HhaI GCGC 6 cut(s) 165, 235, 286, 439, 463, 502
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 4 cut(s) 174, 232, 280, 331
Hin6I GCGC 6 cut(s) 163, 233, 284, 437, 461, 500
HinP1I GCGC 6 cut(s) 163, 233, 284, 437, 461, 500
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 2 cut(s) 237, 546
HpaII CCGG 1 cut(s) 649
HphI GGTGA 4 cut(s) 34, 73, 130, 184
Hpy166II GTNNAC 2 cut(s) 154, 268
Hpy188III TCNNGA 2 cut(s) 517, 566
Hpy8I GTNNAC 2 cut(s) 154, 268
Hpy99I CGWCG 8 cut(s) 163, 227, 356, 395, 419, 512, 518, 572
HpyAV CCTTC 2 cut(s) 124, 208
HpyCH4III ACNGT 2 cut(s) 18, 454
HpyCH4IV ACGT 1 cut(s) 477
HpyF10VI GCNNNNNNNGC 3 cut(s) 241, 362, 499
HpyF3I CTNAG 1 cut(s) 302
HpySE526I ACGT 1 cut(s) 477
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 4 cut(s) 174, 232, 280, 331
HspAI GCGC 6 cut(s) 163, 233, 284, 437, 461, 500
KspI CCGCGG 1 cut(s) 585
Kzo9I GATC 3 cut(s) 321, 330, 504
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 1 cut(s) 534
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 3 cut(s) 23, 448, 547
MalI GATC 3 cut(s) 323, 332, 506
MboI GATC 3 cut(s) 321, 330, 504
MboII GAAGA 5 cut(s) 280, 289, 328, 367, 566
MhlI GDGCHC 2 cut(s) 183, 531
MlyI GAGTC 1 cut(s) 555
MmeI TCCRAC 2 cut(s) 142, 237
MslI CAYNNNNRTG 2 cut(s) 455, 476
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 1 cut(s) 649
MspR9I CCNGG 2 cut(s) 358, 649
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 6 cut(s) 163, 235, 264, 437, 536, 584
MwoI GCNNNNNNNGC 3 cut(s) 241, 362, 499
NciI CCSGG 1 cut(s) 649
NcoI CCATGG 3 cut(s) 170, 228, 276
NdeII GATC 3 cut(s) 321, 330, 504
NlaIII CATG 4 cut(s) 174, 232, 280, 331
NlaIV GGNNCC 3 cut(s) 31, 317, 530
NmuCI GTSAC 2 cut(s) 448, 547
OliI CACNNNNGTG 2 cut(s) 455, 476
PciSI GCTCTTC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 2 cut(s) 423, 513
PfeI GAWTC 1 cut(s) 237
PflFI GACNNNGTC 1 cut(s) 422
PkrI GCNGC 6 cut(s) 6, 49, 167, 283, 538, 583
PleI GAGTC 1 cut(s) 554
PmaCI CACGTG 1 cut(s) 478
PmlI CACGTG 1 cut(s) 478
PpsI GAGTC 1 cut(s) 554
Ppu21I YACGTR 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 356
PspCI CACGTG 1 cut(s) 478
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 3 cut(s) 31, 317, 530
PspPI GGNCC 1 cut(s) 174
PsyI GACNNNGTC 1 cut(s) 422
RsaI GTAC 3 cut(s) 128, 155, 401
RsaNI GTAC 3 cut(s) 127, 154, 400
RseI CAYNNNNRTG 2 cut(s) 455, 476
SacI GAGCTC 1 cut(s) 183
SacII CCGCGG 1 cut(s) 585
SapI GCTCTTC 1 cut(s) 293
SatI GCNGC 6 cut(s) 5, 48, 166, 282, 537, 582
Sau3AI GATC 3 cut(s) 321, 330, 504
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 2 cut(s) 358, 649
SduI GDGCHC 2 cut(s) 183, 531
SetI ASST 9 cut(s) 81, 86, 92, 113, 143, 183, 197, 246, 480
Sfr303I CCGCGG 1 cut(s) 585
SgrBI CCGCGG 1 cut(s) 585
SmiMI CAYNNNNRTG 2 cut(s) 455, 476
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 356, 647
StyI CCWWGG 3 cut(s) 170, 228, 276
TaaI ACNGT 2 cut(s) 18, 454
TaiI ACGT 1 cut(s) 480
TaqI TCGA 5 cut(s) 20, 57, 414, 544, 567
TatI WGTACW 1 cut(s) 399
TauI GCSGC 6 cut(s) 7, 50, 168, 284, 539, 584
TfiI GAWTC 1 cut(s) 237
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 2 cut(s) 448, 547
Tsp45I GTSAC 2 cut(s) 448, 547
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 3 cut(s) 139, 193, 566
TspRI CASTG 1 cut(s) 457
Tth111I GACNNNGTC 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.