FvH4_6g02681

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004291
Physical Location & Seq
Forward (+)
1537335 .. 1538336
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02681.t1

Sequence Viewer

Length: 600 bp
ATGTCGCTAAACCCACCCGCGGGTTACGGCACCAACACCGCCGCTCAAACCTCCGCCACCAAAGACCTCACCCTTGACTCGCCCTCCCAACCCGTGCACCCCACGCGCCGCCCATGGTCCGAGCTCTTCTCCCCCTCCGCCTTCTCCGCCCCTTACAGCTACGCCGACGCCAAGAGCCGCGCGAAGCACAACCTCAGCTACTTCCGCCTCAACTACGCCATGGTGGTGCTCTTCATCGTCTTCCTCAGCCTCTTATGGCACCCCGTCTCCATGATCGTCTTCCTCATCATCCTCATCTACTGGGTCTTCGGTTACTTCTCACGCGCCCCCGGCCCCGTCGTCCTCTTTAACCGGAGCTTCGACGACGGCGCCGTCCTCTTCGCCCTCAGCCTCCTCACCGTGGTGGCGCTTGTGCTCACGCACGTGGGAGTGAATGTGCTGGTCTCTCTGGTTGTTGCCGTGGTGGTTGTGGGCCTCCACGCCGCGTTCCGGGGCACCGAAGACTTGTATTCTCACGAAGAACAAGACTGGGATGCTGAAGGCGGGTTAGTCTCCGTTGTTGGATCAGAGCCGTCGCGCCCGGCCATTACTCGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

200

Amino Acids

21.8

Weight (kDa)

6.27

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 34 - 173 1.3e-46 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 371
AccB1I GGYRCC 4 cut(s) 29, 258, 368, 494
AccBSI CCGCTC 1 cut(s) 44
AccII CGCG 7 cut(s) 20, 106, 180, 182, 324, 485, 577
AclWI GGATC 1 cut(s) 571
AcoI YGGCCR 1 cut(s) 582
AcuI CTGAAG 1 cut(s) 558
AcvI CACGTG 1 cut(s) 424
AcyI GRCGYC 2 cut(s) 168, 369
AfiI CCNNNNNNNGG 5 cut(s) 19, 20, 400, 489, 591
AleI CACNNNNGTG 2 cut(s) 401, 422
AluBI AGCT 4 cut(s) 124, 159, 198, 357
AluI AGCT 4 cut(s) 124, 159, 198, 357
Alw21I GWGCWC 4 cut(s) 99, 126, 231, 417
Alw26I GTCTC 3 cut(s) 271, 448, 556
Alw44I GTGCAC 1 cut(s) 95
AlwI GGATC 1 cut(s) 571
AoxI GGCC 3 cut(s) 331, 472, 582
ApaLI GTGCAC 1 cut(s) 95
AspLEI GCGC 6 cut(s) 108, 182, 326, 371, 409, 579
AspS9I GGNCC 3 cut(s) 117, 332, 472
AsuC2I CCSGG 3 cut(s) 330, 491, 581
AsuHPI GGTGA 2 cut(s) 61, 388
AvaII GGWCC 1 cut(s) 117
BaeGI GKGCMC 2 cut(s) 99, 497
BanI GGYRCC 4 cut(s) 29, 258, 368, 494
BanII GRGCYC 1 cut(s) 126
BbrPI CACGTG 1 cut(s) 424
BbsI GAAGAC 4 cut(s) 232, 271, 298, 507
Bbv12I GWGCWC 4 cut(s) 99, 126, 231, 417
BbvCI CCTCAGC 3 cut(s) 194, 245, 386
BceAI ACGGC 5 cut(s) 43, 356, 382, 443, 556
BcnI CCSGG 3 cut(s) 330, 491, 581
BcoDI GTCTC 3 cut(s) 271, 448, 556
BfoI RGCGCY 2 cut(s) 372, 410
BisI GCNGC 4 cut(s) 42, 109, 178, 483
BlsI GCNGC 4 cut(s) 43, 110, 179, 484
Bme1390I CCNGG 3 cut(s) 330, 491, 581
Bme18I GGWCC 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 117, 332, 472
BmiI GGNNCC 5 cut(s) 31, 260, 334, 370, 496
BmrFI CCNGG 3 cut(s) 330, 491, 581
BmrI ACTGGG 2 cut(s) 310, 538
BmsI GCATC 1 cut(s) 523
BmuI ACTGGG 2 cut(s) 310, 538
BpiI GAAGAC 4 cut(s) 232, 271, 298, 507
BplI GAGNNNNNCTC 2 cut(s) 113, 145
Bpu10I CCTNAGC 3 cut(s) 194, 245, 386
BpuMI CCSGG 3 cut(s) 330, 491, 581
BsaAI YACGTR 1 cut(s) 424
BsaHI GRCGYC 2 cut(s) 168, 369
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 7 cut(s) 18, 113, 219, 328, 399, 459, 490
BsaWI WCCGGW 1 cut(s) 351
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 251, 281, 420, 450
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 20, 400, 489, 591
Bse1I ACTGG 2 cut(s) 305, 533
BseDI CCNNGG 7 cut(s) 18, 113, 219, 328, 399, 459, 490
BseGI GGATG 2 cut(s) 288, 538
BseLI CCNNNNNNNGG 5 cut(s) 19, 20, 400, 489, 591
BseMII CTCAG 3 cut(s) 208, 259, 400
BseNI ACTGG 2 cut(s) 305, 533
BseRI GAGGAG 1 cut(s) 383
BseSI GKGCMC 2 cut(s) 99, 497
Bsh1236I CGCG 7 cut(s) 20, 106, 180, 182, 324, 485, 577
BshFI GGCC 3 cut(s) 333, 474, 584
BshNI GGYRCC 4 cut(s) 29, 258, 368, 494
BsiHKAI GWGCWC 4 cut(s) 99, 126, 231, 417
BsiSI CCGG 4 cut(s) 330, 352, 490, 581
BslI CCNNNNNNNGG 5 cut(s) 19, 20, 400, 489, 591
BsmAI GTCTC 3 cut(s) 271, 448, 556
BsmBI CGTCTC 1 cut(s) 271
BsnI GGCC 3 cut(s) 333, 474, 584
Bso31I GGTCTC 1 cut(s) 448
Bsp1286I GDGCHC 5 cut(s) 99, 126, 231, 417, 497
Bsp143I GATC 2 cut(s) 273, 563
Bsp19I CCATGG 2 cut(s) 113, 219
BspANI GGCC 3 cut(s) 333, 474, 584
BspCNI CTCAG 3 cut(s) 207, 258, 399
BspFNI CGCG 7 cut(s) 20, 106, 180, 182, 324, 485, 577
BspLI GGNNCC 5 cut(s) 31, 260, 334, 370, 496
BspPI GGATC 1 cut(s) 571
BspQI GCTCTTC 2 cut(s) 131, 236
BspT107I GGYRCC 4 cut(s) 29, 258, 368, 494
BspTNI GGTCTC 1 cut(s) 448
BsrBI CCGCTC 1 cut(s) 44
BsrI ACTGG 2 cut(s) 305, 533
BssECI CCNNGG 7 cut(s) 18, 113, 219, 328, 399, 459, 490
BssMI GATC 2 cut(s) 273, 563
BssNI GRCGYC 2 cut(s) 168, 369
BssT1I CCWWGG 2 cut(s) 113, 219
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 3 cut(s) 131, 236, 383
BstACI GRCGYC 2 cut(s) 168, 369
BstBAI YACGTR 1 cut(s) 424
BstDEI CTNAG 3 cut(s) 194, 245, 386
BstDSI CCRYGG 5 cut(s) 18, 113, 219, 399, 459
BstF5I GGATG 2 cut(s) 288, 538
BstFNI CGCG 7 cut(s) 20, 106, 180, 182, 324, 485, 577
BstH2I RGCGCY 2 cut(s) 372, 410
BstHHI GCGC 6 cut(s) 108, 182, 326, 371, 409, 579
BstKTI GATC 2 cut(s) 276, 566
BstMAI GTCTC 3 cut(s) 271, 448, 556
BstMBI GATC 2 cut(s) 273, 563
BstMWI GCNNNNNNNGC 4 cut(s) 103, 146, 204, 330
BstSCI CCNGG 3 cut(s) 328, 489, 579
BstSLI GKGCMC 2 cut(s) 99, 497
BstUI CGCG 7 cut(s) 20, 106, 180, 182, 324, 485, 577
BstV2I GAAGAC 4 cut(s) 232, 271, 298, 507
BsuRI GGCC 3 cut(s) 333, 474, 584
BtgI CCRYGG 5 cut(s) 18, 113, 219, 399, 459
BtsCI GGATG 2 cut(s) 288, 538
CfoI GCGC 6 cut(s) 108, 182, 326, 371, 409, 579
Cfr13I GGNCC 3 cut(s) 117, 332, 472
Cfr42I CCGCGG 1 cut(s) 21
CseI GACGC 1 cut(s) 176
CviAII CATG 3 cut(s) 114, 220, 271
DdeI CTNAG 3 cut(s) 194, 245, 386
DinI GGCGCC 1 cut(s) 370
DpnI GATC 2 cut(s) 275, 565
DpnII GATC 2 cut(s) 273, 563
DrdI GACNNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 1 cut(s) 371
EaeI YGGCCR 1 cut(s) 582
Eam1104I CTCTTC 3 cut(s) 131, 236, 383
EarI CTCTTC 3 cut(s) 131, 236, 383
EciI GGCGGA 4 cut(s) 43, 127, 136, 194
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 2 cut(s) 113, 219
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 558
Eco72I CACGTG 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 124
EcoT14I CCWWGG 2 cut(s) 113, 219
EcoT38I GRGCYC 1 cut(s) 126
EgeI GGCGCC 1 cut(s) 370
EheI GGCGCC 1 cut(s) 370
ErhI CCWWGG 2 cut(s) 113, 219
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 3 cut(s) 117, 223, 274
FaiI YATR 4 cut(s) 115, 221, 256, 272
FatI CATG 3 cut(s) 113, 219, 270
FauI CCCGC 3 cut(s) 13, 25, 536
Fnu4HI GCNGC 4 cut(s) 42, 109, 178, 483
FokI GGATG 2 cut(s) 275, 545
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 4 cut(s) 42, 109, 178, 483
GlaI GCGC 6 cut(s) 107, 181, 325, 370, 408, 578
GluI GCNGC 4 cut(s) 42, 109, 178, 483
HaeII RGCGCY 2 cut(s) 372, 410
HaeIII GGCC 3 cut(s) 333, 474, 584
HapII CCGG 4 cut(s) 330, 352, 490, 581
HgaI GACGC 1 cut(s) 176
HhaI GCGC 6 cut(s) 108, 182, 326, 371, 409, 579
Hin1I GRCGYC 2 cut(s) 168, 369
Hin1II CATG 3 cut(s) 117, 223, 274
Hin6I GCGC 6 cut(s) 106, 180, 324, 369, 407, 577
HinP1I GCGC 6 cut(s) 106, 180, 324, 369, 407, 577
HinfI GANTC 1 cut(s) 77
HpaII CCGG 4 cut(s) 330, 352, 490, 581
HphI GGTGA 2 cut(s) 61, 388
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 2 cut(s) 121, 568
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 5 cut(s) 170, 341, 365, 368, 577
HpyAV CCTTC 2 cut(s) 151, 533
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 146, 204, 330
HpyF3I CTNAG 3 cut(s) 194, 245, 386
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 2 cut(s) 168, 369
Hsp92II CATG 3 cut(s) 117, 223, 274
HspAI GCGC 6 cut(s) 106, 180, 324, 369, 407, 577
KasI GGCGCC 1 cut(s) 368
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 2 cut(s) 273, 563
LguI GCTCTTC 2 cut(s) 131, 236
LmnI GCTCC 1 cut(s) 354
LpnPI CCDG 8 cut(s) 286, 343, 365, 425, 434, 503, 514, 594
LweI GCATC 1 cut(s) 523
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 2 cut(s) 23, 311
MalI GATC 2 cut(s) 275, 565
MbiI CCGCTC 1 cut(s) 44
MboI GATC 2 cut(s) 273, 563
MboII GAAGA 8 cut(s) 118, 223, 232, 271, 298, 370, 512, 530
MhlI GDGCHC 5 cut(s) 99, 126, 231, 417, 497
Mly113I GGCGCC 1 cut(s) 369
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 541
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 3 cut(s) 224, 401, 422
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 4 cut(s) 330, 352, 490, 581
MspR9I CCNGG 3 cut(s) 330, 491, 581
MvnI CGCG 7 cut(s) 20, 106, 180, 182, 324, 485, 577
MwoI GCNNNNNNNGC 4 cut(s) 103, 146, 204, 330
NarI GGCGCC 1 cut(s) 369
NciI CCSGG 3 cut(s) 330, 491, 581
NcoI CCATGG 2 cut(s) 113, 219
NdeII GATC 2 cut(s) 273, 563
NlaIII CATG 3 cut(s) 117, 223, 274
NlaIV GGNNCC 5 cut(s) 31, 260, 334, 370, 496
OliI CACNNNNGTG 2 cut(s) 401, 422
PciSI GCTCTTC 2 cut(s) 131, 236
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PkrI GCNGC 4 cut(s) 43, 110, 179, 484
PleI GAGTC 1 cut(s) 71
PluTI GGCGCC 1 cut(s) 372
PmaCI CACGTG 1 cut(s) 424
PmlI CACGTG 1 cut(s) 424
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 126
PspCI CACGTG 1 cut(s) 424
PspN4I GGNNCC 5 cut(s) 31, 260, 334, 370, 496
PspPI GGNCC 3 cut(s) 117, 332, 472
RseI CAYNNNNRTG 3 cut(s) 224, 401, 422
SacI GAGCTC 1 cut(s) 126
SacII CCGCGG 1 cut(s) 21
SapI GCTCTTC 2 cut(s) 131, 236
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 4 cut(s) 42, 109, 178, 483
Sau3AI GATC 2 cut(s) 273, 563
Sau96I GGNCC 3 cut(s) 117, 332, 472
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 330, 491, 581
SduI GDGCHC 5 cut(s) 99, 126, 231, 417, 497
SetI ASST 8 cut(s) 53, 69, 126, 161, 195, 200, 359, 426
SfaNI GCATC 1 cut(s) 523
SfoI GGCGCC 1 cut(s) 370
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 1 cut(s) 117
SmiMI CAYNNNNRTG 3 cut(s) 224, 401, 422
SspDI GGCGCC 1 cut(s) 368
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 3 cut(s) 328, 489, 579
StyI CCWWGG 2 cut(s) 113, 219
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 426
TaqI TCGA 1 cut(s) 360
TauI GCSGC 4 cut(s) 44, 111, 180, 485
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TspDTI ATGAA 1 cut(s) 223
TspGWI ACGGA 1 cut(s) 544
VneI GTGCAC 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.