MD04G1225900.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004291
Physical Location & Seq
Reverse (-)
30562007 .. 30562663
657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1225900.v1.1.491

Sequence Viewer

Length: 657 bp
ATGCCGCTAAAAACACCGGCGGGTTACGGCGGCACCACCGTCACCGCCGCCCAAATCGACCCGCAAACCTCCAAATACCTCACCCTTACCTCCCGCCCTGTACCCCAGACCTCCTCCCAACCCCTGTACCCAACTCTCAACTCCCAAACCGTGTACCCGACGAGTCGCCCATGGCCCGAGATCTTCTCCCTCACCTCCTTCTCCGTACCCTACAACTACGCCGACGCAATTGCACGAATCAAGCGCAACACCGCCTACTTCCGCGTCAACTACGTCATGGCGACGCTCTTCATCGTCTTCTGCAGCCTCCTCTGGCACCCGATCTCTATGATCGTCTTCCTCATCGTCCTCGTCGCCTGGCTCGCCCTCTACTTCTTCCGCACCACCCCCGTTGTTCTGTTCAATCAGAGCTTCGACGACAGGGTCGTCGCCGTCGTGCTGGGATTGGTCACTGTCGTGGCGCTGGTATTCACTCACGTGGGTTTAAACGTGCTGGTGGCACTGATCGTCGCCGTCGTGGTTATTGGGCTCCACGCGGCGTTTCGCGTGACGGAAGACTTGTTTCTCGACGAGGAGACCGCCGCGGAAAATGGGTTGGTCTCGGTTGTTTCTAGCCAGCAGACCCTCCCCCCGCCGACTTCTTACACCCGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

219

Amino Acids

24.08

Weight (kDa)

7.86

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 53 - 192 5.4e-47 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 422, 425
AccB1I GGYRCC 2 cut(s) 32, 315
AccII CGCG 4 cut(s) 264, 536, 546, 584
AcvI CACGTG 1 cut(s) 478
AfaI GTAC 4 cut(s) 102, 128, 155, 207
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 1 cut(s) 356
AleI CACNNNNGTG 2 cut(s) 455, 476
AluBI AGCT 1 cut(s) 411
AluI AGCT 1 cut(s) 411
Alw26I GTCTC 2 cut(s) 569, 604
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 303
AspLEI GCGC 2 cut(s) 246, 463
AspS9I GGNCC 1 cut(s) 174
AsuC2I CCSGG 1 cut(s) 649
AsuHPI GGTGA 3 cut(s) 34, 73, 184
AvaI CYCGRG 1 cut(s) 176
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BanI GGYRCC 2 cut(s) 32, 315
BanII GRGCYC 1 cut(s) 531
BbrPI CACGTG 1 cut(s) 478
BbsI GAAGAC 3 cut(s) 289, 328, 561
BbvI GCAGC 1 cut(s) 315
BceAI ACGGC 3 cut(s) 43, 416, 497
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 1 cut(s) 358
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 2 cut(s) 569, 604
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 1 cut(s) 301
BfoI RGCGCY 1 cut(s) 464
BglII AGATCT 1 cut(s) 180
BisI GCNGC 6 cut(s) 5, 31, 48, 304, 537, 582
BlsI GCNGC 6 cut(s) 6, 32, 49, 305, 538, 583
Bme1390I CCNGG 2 cut(s) 358, 649
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 3 cut(s) 34, 317, 530
BmrFI CCNGG 2 cut(s) 358, 649
BpiI GAAGAC 3 cut(s) 289, 328, 561
BplI GAGNNNNNCTC 2 cut(s) 170, 202
BpuMI CCSGG 1 cut(s) 649
BsaAI YACGTR 1 cut(s) 478
BsaI GGTCTC 2 cut(s) 569, 604
BsaJI CCNNGG 2 cut(s) 170, 582
Bse118I RCCGGY 1 cut(s) 16
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 2 cut(s) 170, 582
BseRI GAGGAG 3 cut(s) 103, 299, 587
BseXI GCAGC 1 cut(s) 315
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 4 cut(s) 264, 536, 546, 584
BshFI GGCC 1 cut(s) 175
BshNI GGYRCC 2 cut(s) 32, 315
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 17, 649
BsmAI GTCTC 2 cut(s) 569, 604
BsnI GGCC 1 cut(s) 175
Bso31I GGTCTC 2 cut(s) 569, 604
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 4 cut(s) 180, 321, 330, 504
Bsp19I CCATGG 1 cut(s) 170
BspANI GGCC 1 cut(s) 175
BspFNI CGCG 4 cut(s) 264, 536, 546, 584
BspLI GGNNCC 3 cut(s) 34, 317, 530
BspMAI CTGCAG 1 cut(s) 305
BspQI GCTCTTC 1 cut(s) 293
BspT107I GGYRCC 2 cut(s) 32, 315
BspTNI GGTCTC 2 cut(s) 569, 604
BsrFI RCCGGY 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 170, 582
BssMI GATC 4 cut(s) 180, 321, 330, 504
BssT1I CCWWGG 1 cut(s) 170
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 3 cut(s) 40, 151, 454
Bst6I CTCTTC 1 cut(s) 293
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 2 cut(s) 363, 617
BstDSI CCRYGG 2 cut(s) 170, 582
BstFNI CGCG 4 cut(s) 264, 536, 546, 584
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 2 cut(s) 246, 463
BstKTI GATC 4 cut(s) 183, 324, 333, 507
BstMAI GTCTC 2 cut(s) 569, 604
BstMBI GATC 4 cut(s) 180, 321, 330, 504
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 358
BstSCI CCNGG 2 cut(s) 356, 647
BstSFI CTRYAG 1 cut(s) 301
BstUI CGCG 4 cut(s) 264, 536, 546, 584
BstV1I GCAGC 1 cut(s) 315
BstV2I GAAGAC 3 cut(s) 289, 328, 561
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuRI GGCC 1 cut(s) 175
BtgI CCRYGG 2 cut(s) 170, 582
BtsIMutI CAGTG 2 cut(s) 450, 500
Cac8I GCNNGC 2 cut(s) 363, 617
CfoI GCGC 2 cut(s) 246, 463
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 174
Cfr42I CCGCGG 1 cut(s) 585
CseI GACGC 3 cut(s) 233, 253, 292
Csp6I GTAC 4 cut(s) 101, 127, 154, 206
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviAII CATG 2 cut(s) 171, 277
CviJI RGCY 6 cut(s) 175, 306, 361, 411, 529, 615
CviKI_1 RGCY 6 cut(s) 175, 306, 361, 411, 529, 615
CviQI GTAC 4 cut(s) 101, 127, 154, 206
DpnI GATC 4 cut(s) 182, 323, 332, 506
DpnII GATC 4 cut(s) 180, 321, 330, 504
DraI TTTAAA 1 cut(s) 486
DrdI GACNNNNNNGTC 2 cut(s) 422, 425
DseDI GACNNNNNNGTC 2 cut(s) 422, 425
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 531
Eco31I GGTCTC 2 cut(s) 569, 604
Eco72I CACGTG 1 cut(s) 478
Eco88I CYCGRG 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 170
EcoT38I GRGCYC 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 2 cut(s) 174, 280
FaiI YATR 3 cut(s) 172, 278, 329
FatI CATG 2 cut(s) 170, 276
FauI CCCGC 4 cut(s) 13, 69, 101, 639
Fnu4HI GCNGC 6 cut(s) 5, 31, 48, 304, 537, 582
FriOI GRGCYC 1 cut(s) 531
Fsp4HI GCNGC 6 cut(s) 5, 31, 48, 304, 537, 582
FspBI CTAG 1 cut(s) 612
GlaI GCGC 2 cut(s) 245, 462
GluI GCNGC 6 cut(s) 5, 31, 48, 304, 537, 582
GsaI CCCAGC 1 cut(s) 443
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 1 cut(s) 175
HapII CCGG 2 cut(s) 17, 649
HgaI GACGC 3 cut(s) 233, 253, 292
HhaI GCGC 2 cut(s) 246, 463
Hin1II CATG 2 cut(s) 174, 280
Hin6I GCGC 2 cut(s) 244, 461
HinP1I GCGC 2 cut(s) 244, 461
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 2 cut(s) 163, 237
HpaII CCGG 2 cut(s) 17, 649
HphI GGTGA 3 cut(s) 34, 73, 184
Hpy166II GTNNAC 2 cut(s) 154, 268
Hpy188I TCNGA 1 cut(s) 408
Hpy188III TCNNGA 1 cut(s) 566
Hpy8I GTNNAC 2 cut(s) 154, 268
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 3 cut(s) 40, 151, 454
HpyCH4IV ACGT 3 cut(s) 273, 477, 489
HpyCH4V TGCA 2 cut(s) 233, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpySE526I ACGT 3 cut(s) 273, 477, 489
Hsp92II CATG 2 cut(s) 174, 280
HspAI GCGC 2 cut(s) 244, 461
KspI CCGCGG 1 cut(s) 585
Kzo9I GATC 4 cut(s) 180, 321, 330, 504
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 1 cut(s) 315
MaeI CTAG 1 cut(s) 612
MaeII ACGT 3 cut(s) 273, 477, 489
MaeIII GTNAC 4 cut(s) 23, 40, 448, 547
MalI GATC 4 cut(s) 182, 323, 332, 506
MboI GATC 4 cut(s) 180, 321, 330, 504
MboII GAAGA 6 cut(s) 175, 280, 289, 328, 367, 566
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 1 cut(s) 228
MlyI GAGTC 1 cut(s) 172
MseI TTAA 1 cut(s) 485
MslI CAYNNNNRTG 2 cut(s) 455, 476
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 2 cut(s) 17, 649
MspR9I CCNGG 2 cut(s) 358, 649
MssI GTTTAAAC 1 cut(s) 486
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 4 cut(s) 264, 536, 546, 584
MwoI GCNNNNNNNGC 1 cut(s) 362
NciI CCSGG 1 cut(s) 649
NcoI CCATGG 1 cut(s) 170
NdeII GATC 4 cut(s) 180, 321, 330, 504
NlaIII CATG 2 cut(s) 174, 280
NlaIV GGNNCC 3 cut(s) 34, 317, 530
NmuCI GTSAC 3 cut(s) 40, 448, 547
OliI CACNNNNGTG 2 cut(s) 455, 476
PciSI GCTCTTC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 3 cut(s) 423, 432, 513
PfeI GAWTC 1 cut(s) 237
PflFI GACNNNGTC 1 cut(s) 422
PkrI GCNGC 6 cut(s) 6, 32, 49, 305, 538, 583
PleI GAGTC 1 cut(s) 171
PmaCI CACGTG 1 cut(s) 478
PmeI GTTTAAAC 1 cut(s) 486
PmlI CACGTG 1 cut(s) 478
PpsI GAGTC 1 cut(s) 171
Ppu21I YACGTR 1 cut(s) 478
Psp6I CCWGG 1 cut(s) 356
PspCI CACGTG 1 cut(s) 478
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 3 cut(s) 34, 317, 530
PspPI GGNCC 1 cut(s) 174
PstI CTGCAG 1 cut(s) 305
PsuI RGATCY 1 cut(s) 180
PsyI GACNNNGTC 1 cut(s) 422
RsaI GTAC 4 cut(s) 102, 128, 155, 207
RsaNI GTAC 4 cut(s) 101, 127, 154, 206
RseI CAYNNNNRTG 2 cut(s) 455, 476
SacII CCGCGG 1 cut(s) 585
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 6 cut(s) 5, 31, 48, 304, 537, 582
Sau3AI GATC 4 cut(s) 180, 321, 330, 504
Sau96I GGNCC 1 cut(s) 174
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 2 cut(s) 358, 649
SduI GDGCHC 1 cut(s) 531
SetI ASST 9 cut(s) 71, 81, 92, 113, 197, 276, 413, 480, 492
SfcI CTRYAG 1 cut(s) 301
Sfr303I CCGCGG 1 cut(s) 585
SgrAI CRCCGGYG 1 cut(s) 16
SgrBI CCGCGG 1 cut(s) 585
SmiMI CAYNNNNRTG 2 cut(s) 455, 476
Sse9I AATT 1 cut(s) 228
SspMI CTAG 1 cut(s) 612
StyD4I CCNGG 2 cut(s) 356, 647
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 3 cut(s) 40, 151, 454
TaiI ACGT 3 cut(s) 276, 480, 492
TaqI TCGA 3 cut(s) 57, 414, 567
TasI AATT 1 cut(s) 228
TauI GCSGC 5 cut(s) 7, 33, 50, 539, 584
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 2 cut(s) 457, 507
TseFI GTSAC 3 cut(s) 40, 448, 547
TseI GCWGC 1 cut(s) 303
Tsp45I GTSAC 3 cut(s) 40, 448, 547
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 2 cut(s) 193, 566
TspRI CASTG 2 cut(s) 457, 507
Tth111I GACNNNGTC 1 cut(s) 422
XspI CTAG 1 cut(s) 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.