FvH4_2g23050

Chlorophyllase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008747
Physical Location & Seq
Reverse (-)
18908520 .. 18910124
1605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23050.t1

Sequence Viewer

Length: 927 bp
ATGGCATCTGTTTTTGATGAAGGAAAGCTCCCCTTCAAGTTCATTACAGAGGAAACACCATTCTCCTCCTGTTTCGATTCCTCAGCCCCTCCAAAACCTTTGTTAATTGCGACACCAACTGTAAACGGAACTTATCCAGTACTCTTGCTTCTTCATGGCTTCTACCTCCGCAACTACTTCTACAAAGGGATACTCCAACACATAGCCTCTCATGGATTCATAGCTGTTGGACCTCAGTTATACAGCTTGGTGCCTTCAACTGGACCTGAAGAAATTGAATCAACTGCAAAAGTGGTGAACTGGTTACCACAAGGCCTTCAATCTCTTCTGCCTGAAAACGTTGTCGCAGACTTGACCAAAGTTGCTCTATCAGGCCATAGCAGAGGAGGCAAAGCAGCATTTGCACTAGCACTTGGGCATGCCAAGGCTTCTTTGTCCCTTGATTTTTCAGTGCTAATTGGGATTGACCCCGTAGCGGGTGCCAACCAATATTGCAGAACTAACCCCCACATCCTCACCTATGTCCCCTGCTCTTTCGACCTTAACATTCCCATCGCGGTGATTGGCACTGGCCTTGGACCGGAAAAGAAGAACGATTGCATGTCGCAACCGTGCGCTTCCGATGGTGTGAACCACAAGGAGTTCTTCAACGAGAGCAAACCTCCATGTGCACATTTTGTGGCCAAGGACTATGGTCACATGGACATGTTGGACGACGACCCACCTGGCTTGGTTGGAGCCCTGTCGGGATGTTTGTGCAAGAATGGAAGCGGTCCCAAGGACCTAATGAGGAAATCTGTGGGCGGAATTGTTGTGGCTTTTCTGAAGGCATATTTGAATGGTGAAGATGGAGATCTTCTTGCTATTGTAGCTGATCCTGGTCTTTCTCCTGCAAAGCTTGATCCTGTGAAGTTCATCACAGCATGA

Protein Analysis

309

Amino Acids

32.7

Weight (kDa)

5.82

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chlorophyllase PF07224 2 - 305 2.9e-131 Chlorophyllase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 250, 479
AccII CGCG 1 cut(s) 557
AciI CCGC 5 cut(s) 169, 476, 557, 771, 804
AclI AACGTT 1 cut(s) 339
AclWI GGATC 2 cut(s) 869, 896
AcoI YGGCCR 1 cut(s) 681
AcuI CTGAAG 2 cut(s) 288, 845
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 4 cut(s) 260, 475, 476, 580
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 6 cut(s) 37, 258, 278, 320, 649, 838
AjnI CCWGG 2 cut(s) 724, 877
AluBI AGCT 5 cut(s) 28, 224, 246, 872, 898
AluI AGCT 5 cut(s) 28, 224, 246, 872, 898
Alw21I GWGCWC 1 cut(s) 673
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 2 cut(s) 869, 896
AoxI GGCC 4 cut(s) 313, 373, 571, 681
ApaLI GTGCAC 1 cut(s) 669
ApeKI GCWGC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 5 cut(s) 230, 263, 578, 773, 781
AsuHPI GGTGA 4 cut(s) 307, 508, 571, 854
AvaII GGWCC 5 cut(s) 230, 263, 578, 773, 781
BaeGI GKGCMC 1 cut(s) 673
BalI TGGCCA 1 cut(s) 683
BanI GGYRCC 2 cut(s) 250, 479
BanII GRGCYC 1 cut(s) 742
BarI GAAGNNNNNNTAC 2 cut(s) 132, 164
Bbv12I GWGCWC 1 cut(s) 673
BbvCI CCTCAGC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 407
BccI CCATC 3 cut(s) 560, 617, 842
BciT130I CCWGG 2 cut(s) 726, 879
BciVI GTATCC 1 cut(s) 183
BfaI CTAG 1 cut(s) 407
BfuI GTATCC 1 cut(s) 183
BglII AGATCT 1 cut(s) 853
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
BmcAI AGTACT 1 cut(s) 141
Bme1390I CCNGG 2 cut(s) 726, 879
Bme18I GGWCC 5 cut(s) 230, 263, 578, 773, 781
BmgT120I GGNCC 5 cut(s) 230, 263, 578, 773, 781
BmiI GGNNCC 4 cut(s) 252, 481, 739, 775
BmrFI CCNGG 2 cut(s) 726, 879
BmsI GCATC 1 cut(s) 14
BoxI GACNNNNGTC 1 cut(s) 693
BplI GAGNNNNNCTC 2 cut(s) 646, 678
Bpu10I CCTNAGC 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 852
BsaJI CCNNGG 4 cut(s) 423, 574, 684, 777
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 475, 476, 580
Bse1I ACTGG 4 cut(s) 137, 265, 305, 574
Bse8I GATNNNNATC 1 cut(s) 852
BseBI CCWGG 2 cut(s) 726, 879
BseDI CCNNGG 4 cut(s) 423, 574, 684, 777
BseGI GGATG 2 cut(s) 510, 755
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 4 cut(s) 260, 475, 476, 580
BseMII CTCAG 2 cut(s) 96, 248
BseNI ACTGG 4 cut(s) 137, 265, 305, 574
BseRI GAGGAG 2 cut(s) 55, 399
BseSI GKGCMC 1 cut(s) 673
BseXI GCAGC 1 cut(s) 407
Bsh1236I CGCG 1 cut(s) 557
BshFI GGCC 4 cut(s) 315, 375, 573, 683
BshNI GGYRCC 2 cut(s) 250, 479
BsiHKAI GWGCWC 1 cut(s) 673
BsiSI CCGG 1 cut(s) 581
BslFI GGGAC 3 cut(s) 421, 509, 759
BslI CCNNNNNNNGG 4 cut(s) 260, 475, 476, 580
BsmFI GGGAC 3 cut(s) 421, 509, 759
BsnI GGCC 4 cut(s) 315, 375, 573, 683
Bsp1286I GDGCHC 2 cut(s) 673, 742
Bsp143I GATC 3 cut(s) 853, 874, 901
BspACI CCGC 5 cut(s) 169, 476, 557, 771, 804
BspANI GGCC 4 cut(s) 315, 375, 573, 683
BspCNI CTCAG 2 cut(s) 95, 247
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 4 cut(s) 252, 481, 739, 775
BspPI GGATC 2 cut(s) 869, 896
BspT107I GGYRCC 2 cut(s) 250, 479
BsrI ACTGG 4 cut(s) 137, 265, 305, 574
BssECI CCNNGG 4 cut(s) 423, 574, 684, 777
BssMI GATC 3 cut(s) 853, 874, 901
BssT1I CCWWGG 4 cut(s) 423, 574, 684, 777
Bst2UI CCWGG 2 cut(s) 726, 879
Bst4CI ACNGT 2 cut(s) 121, 612
Bst6I CTCTTC 1 cut(s) 330
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 2 cut(s) 82, 234
BstEII GGTNACC 1 cut(s) 303
BstF5I GGATG 2 cut(s) 510, 755
BstFNI CGCG 1 cut(s) 557
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 3 cut(s) 856, 877, 904
BstMBI GATC 3 cut(s) 853, 874, 901
BstMWI GCNNNNNNNGC 3 cut(s) 387, 401, 869
BstNI CCWGG 2 cut(s) 726, 879
BstNSI RCATGY 3 cut(s) 422, 604, 709
BstPAI GACNNNNGTC 1 cut(s) 693
BstPI GGTNACC 1 cut(s) 303
BstSCI CCNGG 2 cut(s) 724, 877
BstSLI GKGCMC 1 cut(s) 673
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 1 cut(s) 407
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuI GTATCC 1 cut(s) 183
BsuRI GGCC 4 cut(s) 315, 375, 573, 683
BtgZI GCGATG 1 cut(s) 538
BtsCI GGATG 2 cut(s) 510, 755
BtsIMutI CAGTG 2 cut(s) 456, 567
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 1 cut(s) 617
Cfr13I GGNCC 5 cut(s) 230, 263, 578, 773, 781
Csp6I GTAC 1 cut(s) 140
CspCI CAANNNNNGTGG 2 cut(s) 711, 746
CviAII CATG 8 cut(s) 155, 212, 419, 601, 666, 700, 706, 924
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 2 cut(s) 82, 234
DpnI GATC 3 cut(s) 855, 876, 903
DpnII GATC 3 cut(s) 853, 874, 901
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 819
Eco130I CCWWGG 4 cut(s) 423, 574, 684, 777
Eco147I AGGCCT 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 742
Eco47I GGWCC 5 cut(s) 230, 263, 578, 773, 781
Eco57I CTGAAG 2 cut(s) 288, 845
Eco91I GGTNACC 1 cut(s) 303
EcoO109I RGGNCCY 1 cut(s) 781
EcoO65I GGTNACC 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 724, 877
EcoT14I CCWWGG 4 cut(s) 423, 574, 684, 777
EcoT38I GRGCYC 1 cut(s) 742
ErhI CCWWGG 4 cut(s) 423, 574, 684, 777
FaeI CATG 8 cut(s) 158, 215, 422, 604, 669, 703, 709, 927
FalI AAGNNNNNCTT 4 cut(s) 17, 49, 629, 661
FaqI GGGAC 3 cut(s) 421, 509, 759
FatI CATG 8 cut(s) 154, 211, 418, 600, 665, 699, 705, 923
FauI CCCGC 1 cut(s) 469
Fnu4HI GCNGC 1 cut(s) 396
FokI GGATG 2 cut(s) 497, 762
FriOI GRGCYC 1 cut(s) 742
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 616
GluI GCNGC 1 cut(s) 396
HaeIII GGCC 4 cut(s) 315, 375, 573, 683
HapII CCGG 1 cut(s) 581
HhaI GCGC 1 cut(s) 617
Hin1II CATG 8 cut(s) 158, 215, 422, 604, 669, 703, 709, 927
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 3 cut(s) 77, 216, 278
HpaII CCGG 1 cut(s) 581
HphI GGTGA 4 cut(s) 307, 508, 571, 854
Hpy166II GTNNAC 4 cut(s) 124, 298, 631, 671
Hpy188I TCNGA 2 cut(s) 622, 825
Hpy188III TCNNGA 1 cut(s) 747
Hpy8I GTNNAC 4 cut(s) 124, 298, 631, 671
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 5 cut(s) 14, 43, 264, 326, 820
HpyCH4III ACNGT 2 cut(s) 121, 612
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 7 cut(s) 287, 404, 495, 600, 671, 759, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 387, 401, 869
HpyF3I CTNAG 2 cut(s) 82, 234
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 8 cut(s) 158, 215, 422, 604, 669, 703, 709, 927
HspAI GCGC 1 cut(s) 615
Kzo9I GATC 3 cut(s) 853, 874, 901
LmnI GCTCC 2 cut(s) 33, 737
Lsp1109I GCAGC 1 cut(s) 407
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 407
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 2 cut(s) 303, 695
MalI GATC 3 cut(s) 855, 876, 903
MboI GATC 3 cut(s) 853, 874, 901
MboII GAAGA 7 cut(s) 143, 281, 317, 601, 637, 848, 857
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 2 cut(s) 673, 742
MlsI TGGCCA 1 cut(s) 683
MluCI AATT 4 cut(s) 105, 273, 456, 807
MluNI TGGCCA 1 cut(s) 683
MmeI TCCRAC 4 cut(s) 208, 220, 690, 715
Mox20I TGGCCA 1 cut(s) 683
MscI TGGCCA 1 cut(s) 683
MseI TTAA 2 cut(s) 104, 543
MslI CAYNNNNRTG 2 cut(s) 557, 704
Msp20I TGGCCA 1 cut(s) 683
MspI CCGG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 726, 879
MvaI CCWGG 2 cut(s) 726, 879
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 3 cut(s) 387, 401, 869
NdeII GATC 3 cut(s) 853, 874, 901
NlaIII CATG 8 cut(s) 158, 215, 422, 604, 669, 703, 709, 927
NlaIV GGNNCC 4 cut(s) 252, 481, 739, 775
NmuCI GTSAC 1 cut(s) 695
NspI RCATGY 3 cut(s) 422, 604, 709
PaeI GCATGC 1 cut(s) 422
PceI AGGCCT 1 cut(s) 315
PciI ACATGT 1 cut(s) 705
PfeI GAWTC 3 cut(s) 77, 216, 278
PkrI GCNGC 1 cut(s) 397
PpuMI RGGWCCY 1 cut(s) 781
PscI ACATGT 1 cut(s) 705
PshAI GACNNNNGTC 1 cut(s) 693
Psp1406I AACGTT 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 781
Psp6I CCWGG 2 cut(s) 724, 877
PspEI GGTNACC 1 cut(s) 303
PspGI CCWGG 2 cut(s) 724, 877
PspN4I GGNNCC 4 cut(s) 252, 481, 739, 775
PspPI GGNCC 5 cut(s) 230, 263, 578, 773, 781
PspPPI RGGWCCY 1 cut(s) 781
PsuI RGATCY 1 cut(s) 853
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
RseI CAYNNNNRTG 2 cut(s) 557, 704
SaqAI TTAA 2 cut(s) 104, 543
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 3 cut(s) 853, 874, 901
Sau96I GGNCC 5 cut(s) 230, 263, 578, 773, 781
ScaI AGTACT 1 cut(s) 141
ScrFI CCNGG 2 cut(s) 726, 879
SduI GDGCHC 2 cut(s) 673, 742
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 5 cut(s) 230, 263, 578, 773, 781
SmiMI CAYNNNNRTG 2 cut(s) 557, 704
SphI GCATGC 1 cut(s) 422
Sse9I AATT 4 cut(s) 105, 273, 456, 807
SseBI AGGCCT 1 cut(s) 315
SsiI CCGC 5 cut(s) 169, 476, 557, 771, 804
SspI AATATT 1 cut(s) 491
SspMI CTAG 1 cut(s) 407
StuI AGGCCT 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 724, 877
StyI CCWWGG 4 cut(s) 423, 574, 684, 777
TaaI ACNGT 2 cut(s) 121, 612
TaiI ACGT 1 cut(s) 342
TaqI TCGA 2 cut(s) 75, 537
TasI AATT 4 cut(s) 105, 273, 456, 807
TatI WGTACW 1 cut(s) 139
TfiI GAWTC 3 cut(s) 77, 216, 278
Tru1I TTAA 2 cut(s) 104, 543
Tru9I TTAA 2 cut(s) 104, 543
TscAI CASTG 2 cut(s) 456, 574
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 5 cut(s) 31, 33, 143, 208, 904
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 456, 574
VneI GTGCAC 1 cut(s) 669
VpaK11BI GGWCC 5 cut(s) 230, 263, 578, 773, 781
XceI RCATGY 3 cut(s) 422, 604, 709
XspI CTAG 1 cut(s) 407
ZrmI AGTACT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.