Rw6G027700

Chlorophyllase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008747
Physical Location & Seq
Reverse (-)
51635902 .. 51637063
1162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027700.1

Sequence Viewer

Length: 927 bp
ATGGCATATGTTTTTGATGAAGGAAAGTTCCCCTTTAAATTCATTACAGAGGAAACTCCATTTTCTTCCTGTTTCGATTCCTCAGCCCCTCCAAAACCATTGTTAATTGCAACACCAACTGTAAGCGGCACTTATCCAGTACTCTTGCTTCTTCATGGCTTCTACCTTCGCAACTACTTCTACAAAGGAATACTCCAACACATAGCCTCTCATGGATTTATAGTAGTTGGACCTCAGTTATATAGCTTGGTGCCGGCAACTGGACCTGAAGAAATTGAATCAGCTGCAAAAGTGGTAAACTGGTTACCAGAAGGCCTTCAATCTCTTCTGCCTGAAAATGTTGTCGCGGACTTGACCAAATTTGCTCTCTCAGGCCACAGTAGAGGAGGCAAAGCAGCATTTGCACTAGCACTTGGATATGCCAAAGCTACTTTGTCCCTCAAATTTTCATTGCTAATTGGGATTGACCCTGTCGCAGGCGCCAACCAATATTGCAGAACTAATCCCCACATCCTCACCTATGTCCCTCGCTCTTTCGACCTGAATATTCCCGTCGCGGTAATTGGCACTGGCCTTGGACCGGAAAAGAAGAACGATTGCATGTCGCAACCGTGTGCTCCCGAGGGTTTGAACCACAAGGAGTTCTTCAACGAGAGCAAACCCCCATGTGCACATTTTGTGGCCAAGGACTATGGTCACATGGACATGTTGGACGACGATCTACCGGGCGTGGTTGGAGCCATGTCAGGATGTATGTGTAAAAATGGAAGCGGTCCGAAGGACCTAATGAGGAAATCTGTGGGCGGAATTGTTGTGGCTTTTTTGAATGCATATTTGAATGGTGAAGATGGAGATCTTCTTGCTATTGTAGCTGATCCTGCTCTTTCTCCTGCAAGGCTTGAACCTGCGGAGTTCATCAATGCGTAA

Protein Analysis

308

Amino Acids

33.02

Weight (kDa)

5.6

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chlorophyllase PF07224 3 - 306 3.5e-133 Chlorophyllase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 913
AccB1I GGYRCC 2 cut(s) 250, 479
AccII CGCG 2 cut(s) 347, 557
AciI CCGC 6 cut(s) 126, 347, 557, 771, 804, 908
AclWI GGATC 1 cut(s) 869
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 3 cut(s) 38, 359, 443
AcuI CTGAAG 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 480
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 3 cut(s) 260, 476, 580
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 7 cut(s) 278, 320, 631, 649, 826, 838, 902
AluBI AGCT 4 cut(s) 246, 284, 428, 872
AluI AGCT 4 cut(s) 246, 284, 428, 872
Alw21I GWGCWC 2 cut(s) 619, 673
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 1 cut(s) 869
Ama87I CYCGRG 1 cut(s) 620
AoxI GGCC 4 cut(s) 313, 373, 571, 681
ApaLI GTGCAC 1 cut(s) 669
ApeKI GCWGC 2 cut(s) 284, 395
ApoI RAATTY 3 cut(s) 38, 359, 443
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 5 cut(s) 230, 263, 578, 773, 781
AsuC2I CCSGG 1 cut(s) 726
AsuHPI GGTGA 2 cut(s) 508, 854
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 5 cut(s) 230, 263, 578, 773, 781
BaeGI GKGCMC 1 cut(s) 673
BalI TGGCCA 1 cut(s) 683
BanI GGYRCC 2 cut(s) 250, 479
BarI GAAGNNNNNNTAC 2 cut(s) 132, 164
Bbv12I GWGCWC 2 cut(s) 619, 673
BbvCI CCTCAGC 1 cut(s) 82
BbvI GCAGC 2 cut(s) 271, 407
BccI CCATC 1 cut(s) 842
BcnI CCSGG 1 cut(s) 726
BfaI CTAG 1 cut(s) 407
BfoI RGCGCY 1 cut(s) 483
BfuAI ACCTGC 1 cut(s) 913
BglII AGATCT 1 cut(s) 853
BisI GCNGC 3 cut(s) 127, 285, 396
BlsI GCNGC 3 cut(s) 128, 286, 397
BmcAI AGTACT 1 cut(s) 141
Bme1390I CCNGG 1 cut(s) 726
Bme18I GGWCC 5 cut(s) 230, 263, 578, 773, 781
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 5 cut(s) 230, 263, 578, 773, 781
BmiI GGNNCC 3 cut(s) 252, 481, 739
BmrFI CCNGG 1 cut(s) 726
BoxI GACNNNNGTC 1 cut(s) 693
Bpu10I CCTNAGC 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 726
BsaBI GATNNNNATC 1 cut(s) 852
BsaHI GRCGYC 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 574, 621, 684
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 476, 580
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 4 cut(s) 137, 265, 305, 574
Bse3DI GCAATG 1 cut(s) 449
Bse8I GATNNNNATC 1 cut(s) 852
BseDI CCNNGG 3 cut(s) 574, 621, 684
BseGI GGATG 2 cut(s) 510, 755
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 3 cut(s) 260, 476, 580
BseMI GCAATG 1 cut(s) 449
BseMII CTCAG 3 cut(s) 96, 248, 384
BseNI ACTGG 4 cut(s) 137, 265, 305, 574
BseRI GAGGAG 1 cut(s) 399
BseSI GKGCMC 1 cut(s) 673
BseXI GCAGC 2 cut(s) 271, 407
Bsh1236I CGCG 2 cut(s) 347, 557
BshFI GGCC 4 cut(s) 315, 375, 573, 683
BshNI GGYRCC 2 cut(s) 250, 479
BsiHKAI GWGCWC 2 cut(s) 619, 673
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 3 cut(s) 254, 581, 725
BslFI GGGAC 2 cut(s) 421, 509
BslI CCNNNNNNNGG 3 cut(s) 260, 476, 580
BsmFI GGGAC 2 cut(s) 421, 509
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 4 cut(s) 315, 375, 573, 683
BsoBI CYCGRG 1 cut(s) 620
Bsp1286I GDGCHC 2 cut(s) 619, 673
Bsp143I GATC 3 cut(s) 718, 853, 874
BspACI CCGC 6 cut(s) 126, 347, 557, 771, 804, 908
BspANI GGCC 4 cut(s) 315, 375, 573, 683
BspCNI CTCAG 3 cut(s) 95, 247, 383
BspFNI CGCG 2 cut(s) 347, 557
BspLI GGNNCC 3 cut(s) 252, 481, 739
BspMI ACCTGC 1 cut(s) 913
BspPI GGATC 1 cut(s) 869
BspT107I GGYRCC 2 cut(s) 250, 479
BsrDI GCAATG 1 cut(s) 449
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 4 cut(s) 137, 265, 305, 574
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 3 cut(s) 574, 621, 684
BssMI GATC 3 cut(s) 718, 853, 874
BssNI GRCGYC 1 cut(s) 480
BssT1I CCWWGG 2 cut(s) 574, 684
Bst4CI ACNGT 3 cut(s) 121, 380, 612
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 480
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 255, 478
BstDEI CTNAG 3 cut(s) 82, 234, 370
BstEII GGTNACC 1 cut(s) 303
BstENI CCTNNNNNAGG 1 cut(s) 474
BstF5I GGATG 2 cut(s) 510, 755
BstFNI CGCG 2 cut(s) 347, 557
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 3 cut(s) 721, 856, 877
BstMBI GATC 3 cut(s) 718, 853, 874
BstMWI GCNNNNNNNGC 3 cut(s) 401, 869, 878
BstNSI RCATGY 2 cut(s) 604, 709
BstPAI GACNNNNGTC 1 cut(s) 693
BstPI GGTNACC 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 724
BstSLI GKGCMC 1 cut(s) 673
BstUI CGCG 2 cut(s) 347, 557
BstV1I GCAGC 2 cut(s) 271, 407
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuRI GGCC 4 cut(s) 315, 375, 573, 683
BtsCI GGATG 2 cut(s) 510, 755
BtsIMutI CAGTG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 913
Cac8I GCNNGC 2 cut(s) 255, 478
CfoI GCGC 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 5 cut(s) 230, 263, 578, 773, 781
CpoI CGGWCCG 1 cut(s) 773
Csp6I GTAC 1 cut(s) 140
CspI CGGWCCG 1 cut(s) 773
CviAII CATG 7 cut(s) 155, 212, 601, 666, 700, 706, 742
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 3 cut(s) 82, 234, 370
DinI GGCGCC 1 cut(s) 481
DpnI GATC 3 cut(s) 720, 855, 876
DpnII GATC 3 cut(s) 718, 853, 874
DraI TTTAAA 1 cut(s) 37
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 819
Eco130I CCWWGG 2 cut(s) 574, 684
Eco147I AGGCCT 1 cut(s) 315
Eco47I GGWCC 5 cut(s) 230, 263, 578, 773, 781
Eco57I CTGAAG 1 cut(s) 288
Eco88I CYCGRG 1 cut(s) 620
Eco91I GGTNACC 1 cut(s) 303
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 781
EcoO65I GGTNACC 1 cut(s) 303
EcoT14I CCWWGG 2 cut(s) 574, 684
EcoT22I ATGCAT 1 cut(s) 832
EgeI GGCGCC 1 cut(s) 481
EheI GGCGCC 1 cut(s) 481
ErhI CCWWGG 2 cut(s) 574, 684
FaeI CATG 7 cut(s) 158, 215, 604, 669, 703, 709, 745
FalI AAGNNNNNCTT 6 cut(s) 17, 49, 115, 147, 629, 661
FaqI GGGAC 2 cut(s) 421, 509
FatI CATG 7 cut(s) 154, 211, 600, 665, 699, 705, 741
FauNDI CATATG 1 cut(s) 7
Fnu4HI GCNGC 3 cut(s) 127, 285, 396
FokI GGATG 2 cut(s) 497, 762
Fsp4HI GCNGC 3 cut(s) 127, 285, 396
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 481
GluI GCNGC 3 cut(s) 127, 285, 396
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 4 cut(s) 315, 375, 573, 683
HapII CCGG 3 cut(s) 254, 581, 725
HhaI GCGC 1 cut(s) 482
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 7 cut(s) 158, 215, 604, 669, 703, 709, 745
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HinfI GANTC 2 cut(s) 77, 278
HpaII CCGG 3 cut(s) 254, 581, 725
HphI GGTGA 2 cut(s) 508, 854
Hpy166II GTNNAC 2 cut(s) 298, 671
Hpy188I TCNGA 1 cut(s) 777
Hpy188III TCNNGA 2 cut(s) 620, 747
Hpy8I GTNNAC 2 cut(s) 298, 671
Hpy99I CGWCG 2 cut(s) 557, 719
HpyAV CCTTC 5 cut(s) 14, 176, 305, 326, 772
HpyCH4III ACNGT 3 cut(s) 121, 380, 612
HpyCH4V TGCA 8 cut(s) 110, 287, 404, 495, 600, 671, 830, 893
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 869, 878
HpyF3I CTNAG 3 cut(s) 82, 234, 370
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 7 cut(s) 158, 215, 604, 669, 703, 709, 745
HspAI GCGC 1 cut(s) 480
KasI GGCGCC 1 cut(s) 479
KroI GCCGGC 1 cut(s) 253
KroNI GCCGGC 1 cut(s) 255
Kzo9I GATC 3 cut(s) 718, 853, 874
LmnI GCTCC 2 cut(s) 622, 737
Lsp1109I GCAGC 2 cut(s) 271, 407
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 2 cut(s) 303, 695
MalI GATC 3 cut(s) 720, 855, 876
MboI GATC 3 cut(s) 718, 853, 874
MboII GAAGA 8 cut(s) 57, 143, 281, 317, 601, 637, 848, 857
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 2 cut(s) 619, 673
MlsI TGGCCA 1 cut(s) 683
MluCI AATT 8 cut(s) 38, 105, 273, 359, 443, 456, 561, 807
MluNI TGGCCA 1 cut(s) 683
Mly113I GGCGCC 1 cut(s) 480
MmeI TCCRAC 4 cut(s) 208, 220, 690, 715
Mox20I TGGCCA 1 cut(s) 683
Mph1103I ATGCAT 1 cut(s) 832
MroNI GCCGGC 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 1 cut(s) 683
MseI TTAA 2 cut(s) 36, 104
MslI CAYNNNNRTG 1 cut(s) 704
Msp20I TGGCCA 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 3 cut(s) 254, 581, 725
MspR9I CCNGG 1 cut(s) 726
Mva1269I GAATGC 1 cut(s) 832
MvnI CGCG 2 cut(s) 347, 557
MwoI GCNNNNNNNGC 3 cut(s) 401, 869, 878
NaeI GCCGGC 1 cut(s) 255
NarI GGCGCC 1 cut(s) 480
NciI CCSGG 1 cut(s) 726
NdeI CATATG 1 cut(s) 7
NdeII GATC 3 cut(s) 718, 853, 874
NgoMIV GCCGGC 1 cut(s) 253
NlaIII CATG 7 cut(s) 158, 215, 604, 669, 703, 709, 745
NlaIV GGNNCC 3 cut(s) 252, 481, 739
NmuCI GTSAC 1 cut(s) 695
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 2 cut(s) 604, 709
PceI AGGCCT 1 cut(s) 315
PciI ACATGT 1 cut(s) 705
PctI GAATGC 1 cut(s) 832
PdiI GCCGGC 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 2 cut(s) 77, 278
PflFI GACNNNGTC 1 cut(s) 470
PkrI GCNGC 3 cut(s) 128, 286, 397
PluTI GGCGCC 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 781
PscI ACATGT 1 cut(s) 705
PshAI GACNNNNGTC 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 781
PspEI GGTNACC 1 cut(s) 303
PspN4I GGNNCC 3 cut(s) 252, 481, 739
PspPI GGNCC 5 cut(s) 230, 263, 578, 773, 781
PspPPI RGGWCCY 1 cut(s) 781
PsuI RGATCY 1 cut(s) 853
PsyI GACNNNGTC 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
RseI CAYNNNNRTG 1 cut(s) 704
Rsr2I CGGWCCG 1 cut(s) 773
RsrII CGGWCCG 1 cut(s) 773
SaqAI TTAA 2 cut(s) 36, 104
SatI GCNGC 3 cut(s) 127, 285, 396
Sau3AI GATC 3 cut(s) 718, 853, 874
Sau96I GGNCC 5 cut(s) 230, 263, 578, 773, 781
ScaI AGTACT 1 cut(s) 141
ScrFI CCNGG 1 cut(s) 726
SduI GDGCHC 2 cut(s) 619, 673
SfoI GGCGCC 1 cut(s) 481
SinI GGWCC 5 cut(s) 230, 263, 578, 773, 781
SmiMI CAYNNNNRTG 1 cut(s) 704
Sse9I AATT 8 cut(s) 38, 105, 273, 359, 443, 456, 561, 807
SseBI AGGCCT 1 cut(s) 315
SsiI CCGC 6 cut(s) 126, 347, 557, 771, 804, 908
SspDI GGCGCC 1 cut(s) 479
SspI AATATT 2 cut(s) 491, 547
SspMI CTAG 1 cut(s) 407
StuI AGGCCT 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 724
StyI CCWWGG 2 cut(s) 574, 684
TaaI ACNGT 3 cut(s) 121, 380, 612
TaqI TCGA 2 cut(s) 75, 537
TasI AATT 8 cut(s) 38, 105, 273, 359, 443, 456, 561, 807
TatI WGTACW 1 cut(s) 139
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 77, 278
Tru1I TTAA 2 cut(s) 36, 104
Tru9I TTAA 2 cut(s) 36, 104
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 2 cut(s) 284, 395
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 5 cut(s) 31, 33, 143, 438, 904
TspRI CASTG 1 cut(s) 574
Tth111I GACNNNGTC 1 cut(s) 470
VneI GTGCAC 1 cut(s) 669
VpaK11BI GGWCC 5 cut(s) 230, 263, 578, 773, 781
XagI CCTNNNNNAGG 1 cut(s) 474
XapI RAATTY 3 cut(s) 38, 359, 443
XceI RCATGY 2 cut(s) 604, 709
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 1 cut(s) 407
ZrmI AGTACT 1 cut(s) 141
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.