MD03G1259100.v1.1

Chlorophyllase-1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008747
Physical Location & Seq
Forward (+)
34618317 .. 34621012
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1259100.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGCACTTGTGGAAAAAAATATGTCTTCTGCTCAGCCTGCACTGACTACATCTGTTTTTAATCTAGGAAATTGCCCCTTCAAATGCATCACGGAGGAGACACCACTGTCTTCCTGCTTTGATTCTTCAGCACCTCCAAAGCCCTTGTTCATTGTTACACCGACGGTAGCAGGCACTTACCCTCTAATCTTGCTTCATCACGGCTTCTACCTCCGCAACTACTTCTACAAGGAGTTACTCCAGCACATAGCTTCACATGGATTCATAGCTGTAGCACCTCAGTTATGTGGGGTTGTGCCTCCATGTGGACCTGAAGAAGTAGAGTCGGGAGCAAAAGTCATAAACTGGTTGCCAAAAGGCCTCCAACCTCTGCTGCCAGAAAATGTTGTAGCAGACTTCACCAAATTTGCTCTTGCAGGCCACAGCAGAGGTGGGAAAACAGCATTTGCAATAGCACTTGGGCGTGCCAAAACTTCCCTGTCCCTCAAAATTTCACTCCTAATAGGCATTGATCCTGTGGCCGGTGCAAATCGATACTGCAGAACACGTCCTCGTATCCTCACCTATTCCCCTCAATCTTTCGACCTCTCGATTCCAGTCATAGTGATCGGCGCTGGCCTGGGACCGGAAGGTAAAAATGCTTGCATGTCAAAACCTTGTGCCCCAGATGGCGTGAACCACAAGGATTTCTTCTATGAGTGCAAGCCTCCATGTGCACATTTTGTTGTGAAGGACTATGGTCACATGGACATGTTGGACGACGATCCACAGGGCATGGTTGGGGCAGTGTCGGGGTGCATGTGTAAAAACGGGACAGGACCGAGGGAGCTAATGAGGAAGACTGTGGGTGGGATTGTTGTGGCGTTTCTGAATGCTTATTTGAATGGTGATGATAGGGATTTGGTTGCCATTGTTGGTGATCCTGATAGTTCTCCTGCAAAGCTTGAACCTGTGGAGTTCATTAGAGCATAA

Protein Analysis

324

Amino Acids

34.62

Weight (kDa)

7.04

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chlorophyllase PF07224 16 - 321 4.1e-131 Chlorophyllase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 106
AciI CCGC 1 cut(s) 214
AclWI GGATC 3 cut(s) 506, 758, 914
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 2 cut(s) 404, 489
AcuI CTGAAG 2 cut(s) 111, 333
AfiI CCNNNNNNNGG 3 cut(s) 305, 521, 625
AflIII ACRYGT 2 cut(s) 545, 750
AgsI TTSAA 3 cut(s) 82, 883, 947
AjiI CACGTC 1 cut(s) 548
AjnI CCWGG 1 cut(s) 618
AluBI AGCT 4 cut(s) 251, 269, 829, 943
AluI AGCT 4 cut(s) 251, 269, 829, 943
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 1 cut(s) 92
Alw44I GTGCAC 1 cut(s) 714
AlwI GGATC 3 cut(s) 506, 758, 914
AoxI GGCC 4 cut(s) 358, 418, 519, 616
ApaLI GTGCAC 1 cut(s) 714
ApeKI GCWGC 1 cut(s) 373
ApoI RAATTY 2 cut(s) 404, 489
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 3 cut(s) 308, 623, 818
AsuHPI GGTGA 4 cut(s) 391, 553, 899, 929
AvaII GGWCC 3 cut(s) 308, 623, 818
BaeGI GKGCMC 2 cut(s) 664, 718
BbsI GAAGAC 3 cut(s) 18, 102, 845
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 1 cut(s) 360
BccI CCATC 1 cut(s) 662
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 620
BciVI GTATCC 1 cut(s) 566
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 65
BfmI CTRYAG 2 cut(s) 270, 538
BfoI RGCGCY 1 cut(s) 615
BfuI GTATCC 1 cut(s) 566
BisI GCNGC 1 cut(s) 374
BlpI GCTNAGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 375
Bme1390I CCNGG 1 cut(s) 620
Bme18I GGWCC 3 cut(s) 308, 623, 818
BmgBI CACGTC 1 cut(s) 548
BmgT120I GGNCC 3 cut(s) 308, 623, 818
BmiI GGNNCC 1 cut(s) 624
BmrFI CCNGG 1 cut(s) 620
BmsI GCATC 1 cut(s) 96
BoxI GACNNNNGTC 1 cut(s) 738
BpiI GAAGAC 3 cut(s) 18, 102, 845
BpmI CTGGAG 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 532
BsaJI CCNNGG 2 cut(s) 619, 821
BsaWI WCCGGW 1 cut(s) 625
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 305, 521, 625
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 2 cut(s) 350, 596
BseBI CCWGG 1 cut(s) 620
BseCI ATCGAT 1 cut(s) 532
BseDI CCNNGG 2 cut(s) 619, 821
BseLI CCNNNNNNNGG 3 cut(s) 305, 521, 625
BseMII CTCAG 2 cut(s) 47, 293
BseNI ACTGG 2 cut(s) 350, 596
BseRI GAGGAG 1 cut(s) 110
BseSI GKGCMC 2 cut(s) 664, 718
BseXI GCAGC 1 cut(s) 360
BsgI GTGCAG 1 cut(s) 24
BshFI GGCC 4 cut(s) 360, 420, 521, 618
BshVI ATCGAT 1 cut(s) 532
BsiHKAI GWGCWC 1 cut(s) 718
BsiSI CCGG 2 cut(s) 522, 626
BslFI GGGAC 3 cut(s) 466, 636, 826
BslI CCNNNNNNNGG 3 cut(s) 305, 521, 625
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 3 cut(s) 466, 636, 826
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 4 cut(s) 360, 420, 521, 618
Bsp1286I GDGCHC 2 cut(s) 664, 718
Bsp143I GATC 4 cut(s) 511, 606, 763, 919
Bsp1720I GCTNAGC 1 cut(s) 33
BspACI CCGC 1 cut(s) 214
BspANI GGCC 4 cut(s) 360, 420, 521, 618
BspCNI CTCAG 2 cut(s) 46, 292
BspDI ATCGAT 1 cut(s) 532
BspLI GGNNCC 1 cut(s) 624
BspMAI CTGCAG 1 cut(s) 542
BspPI GGATC 3 cut(s) 506, 758, 914
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 2 cut(s) 350, 596
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 2 cut(s) 619, 821
BssMI GATC 4 cut(s) 511, 606, 763, 919
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 3 cut(s) 108, 166, 844
BstC8I GCNNGC 7 cut(s) 39, 172, 418, 465, 616, 643, 704
BstDEI CTNAG 2 cut(s) 33, 279
BstH2I RGCGCY 1 cut(s) 615
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 4 cut(s) 514, 609, 766, 922
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 4 cut(s) 511, 606, 763, 919
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 1 cut(s) 620
BstNSI RCATGY 3 cut(s) 649, 754, 802
BstPAI GACNNNNGTC 1 cut(s) 738
BstSCI CCNGG 1 cut(s) 618
BstSFI CTRYAG 2 cut(s) 270, 538
BstSLI GKGCMC 2 cut(s) 664, 718
BstV1I GCAGC 1 cut(s) 360
BstV2I GAAGAC 3 cut(s) 18, 102, 845
Bsu15I ATCGAT 1 cut(s) 532
BsuI GTATCC 1 cut(s) 566
BsuRI GGCC 4 cut(s) 360, 420, 521, 618
BsuTUI ATCGAT 1 cut(s) 532
BtrI CACGTC 1 cut(s) 548
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 3 cut(s) 41, 104, 792
Cac8I GCNNGC 7 cut(s) 39, 172, 418, 465, 616, 643, 704
CfoI GCGC 1 cut(s) 614
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 3 cut(s) 308, 623, 818
ClaI ATCGAT 1 cut(s) 532
CviAII CATG 8 cut(s) 257, 303, 646, 711, 745, 751, 775, 799
DdeI CTNAG 2 cut(s) 33, 279
DpnI GATC 4 cut(s) 513, 608, 765, 921
DpnII GATC 4 cut(s) 511, 606, 763, 919
DrdI GACNNNNNNGTC 1 cut(s) 106
DseDI GACNNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 519
Eco147I AGGCCT 1 cut(s) 360
Eco47I GGWCC 3 cut(s) 308, 623, 818
Eco57I CTGAAG 2 cut(s) 111, 333
EcoRII CCWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 89
FaeI CATG 8 cut(s) 260, 306, 649, 714, 748, 754, 778, 802
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 3 cut(s) 466, 636, 826
FatI CATG 8 cut(s) 256, 302, 645, 710, 744, 750, 774, 798
Fnu4HI GCNGC 1 cut(s) 374
Fsp4HI GCNGC 1 cut(s) 374
FspBI CTAG 1 cut(s) 65
GlaI GCGC 1 cut(s) 613
GluI GCNGC 1 cut(s) 374
GsuI CTGGAG 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 615
HaeIII GGCC 4 cut(s) 360, 420, 521, 618
HapII CCGG 2 cut(s) 522, 626
HhaI GCGC 1 cut(s) 614
Hin1II CATG 8 cut(s) 260, 306, 649, 714, 748, 754, 778, 802
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HindIII AAGCTT 1 cut(s) 941
HinfI GANTC 4 cut(s) 122, 261, 323, 592
HpaII CCGG 2 cut(s) 522, 626
HphI GGTGA 4 cut(s) 391, 553, 899, 929
Hpy166II GTNNAC 3 cut(s) 308, 676, 716
Hpy188I TCNGA 1 cut(s) 870
Hpy188III TCNNGA 3 cut(s) 327, 589, 923
Hpy8I GTNNAC 3 cut(s) 308, 676, 716
Hpy99I CGWCG 2 cut(s) 166, 764
HpyAV CCTTC 3 cut(s) 88, 623, 724
HpyCH4III ACNGT 3 cut(s) 108, 166, 844
HpyCH4IV ACGT 1 cut(s) 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 2 cut(s) 33, 279
HpySE526I ACGT 1 cut(s) 547
Hsp92II CATG 8 cut(s) 260, 306, 649, 714, 748, 754, 778, 802
HspAI GCGC 1 cut(s) 612
Kzo9I GATC 4 cut(s) 511, 606, 763, 919
LmnI GCTCC 2 cut(s) 329, 826
Lsp1109I GCAGC 1 cut(s) 360
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 65
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 3 cut(s) 154, 234, 740
MalI GATC 4 cut(s) 513, 608, 765, 921
MboI GATC 4 cut(s) 511, 606, 763, 919
MboII GAAGA 6 cut(s) 18, 102, 117, 326, 682, 850
MhlI GDGCHC 2 cut(s) 664, 718
MluCI AATT 3 cut(s) 70, 404, 489
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 388, 735
Mph1103I ATGCAT 1 cut(s) 89
MseI TTAA 1 cut(s) 60
MslI CAYNNNNRTG 1 cut(s) 749
MspI CCGG 2 cut(s) 522, 626
MspR9I CCNGG 1 cut(s) 620
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 1 cut(s) 620
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 4 cut(s) 511, 606, 763, 919
NlaIII CATG 8 cut(s) 260, 306, 649, 714, 748, 754, 778, 802
NlaIV GGNNCC 1 cut(s) 624
NmuCI GTSAC 1 cut(s) 740
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 3 cut(s) 649, 754, 802
PceI AGGCCT 1 cut(s) 360
PciI ACATGT 1 cut(s) 750
PctI GAATGC 1 cut(s) 877
PfeI GAWTC 3 cut(s) 122, 261, 592
PkrI GCNGC 1 cut(s) 375
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PscI ACATGT 1 cut(s) 750
PshAI GACNNNNGTC 1 cut(s) 738
Psp6I CCWGG 1 cut(s) 618
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 1 cut(s) 624
PspPI GGNCC 3 cut(s) 308, 623, 818
PstI CTGCAG 1 cut(s) 542
RseI CAYNNNNRTG 1 cut(s) 749
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 1 cut(s) 374
Sau3AI GATC 4 cut(s) 511, 606, 763, 919
Sau96I GGNCC 3 cut(s) 308, 623, 818
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 620
SduI GDGCHC 2 cut(s) 664, 718
SfaNI GCATC 1 cut(s) 96
SfcI CTRYAG 2 cut(s) 270, 538
SinI GGWCC 3 cut(s) 308, 623, 818
SmiMI CAYNNNNRTG 1 cut(s) 749
Sse9I AATT 3 cut(s) 70, 404, 489
SseBI AGGCCT 1 cut(s) 360
SsiI CCGC 1 cut(s) 214
SspMI CTAG 1 cut(s) 65
StuI AGGCCT 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 618
TaaI ACNGT 3 cut(s) 108, 166, 844
TaiI ACGT 1 cut(s) 550
TaqI TCGA 3 cut(s) 532, 582, 590
TaqII GACCGA 1 cut(s) 835
TasI AATT 3 cut(s) 70, 404, 489
TfiI GAWTC 3 cut(s) 122, 261, 592
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 3 cut(s) 48, 111, 792
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 1 cut(s) 373
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 4 cut(s) 139, 185, 253, 949
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 3 cut(s) 48, 111, 792
VneI GTGCAC 1 cut(s) 714
VpaK11BI GGWCC 3 cut(s) 308, 623, 818
XapI RAATTY 2 cut(s) 404, 489
XceI RCATGY 3 cut(s) 649, 754, 802
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XspI CTAG 1 cut(s) 65
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.