Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002658

Physical Location & Seq

Genomic Coordinates Reverse (-)

19088054 .. 19098047

Gene Structure 9994 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g23330.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5217 bp

ATGGAGGGGTTGAGATCTGGCAGGTTGAAGGTTGAGAGAGAGAAGCAAACAGTGGGACAGGAGAATAACGGTGGGGATGAGCCGGAATTTGATGATGATAAGGAGAAGAAGGTGAATGGTGTAGTGCCAATTATGGGGAGGGTTTTAAGGTCACGGGTTGTGGGGAATGGTGGTGGTGAGGGAGGAGGGGAGGTTGGGAAGAGGAGGAGGTTGGTGGATGATGGGGAGGGTAAGAATGGGAAGGAAAAGAAGTGGGATGGTAATGTGGGCATTGGTGGGAGGGTTCTGCGGTCGAGGAGTGTGGTGAATGGTGGGGGTGACAAGGGGGAGGGAGATGGTGGTGAGATGGAGGATGTGGGAGTTGTGGAGAAAAGGAAGAGGCAGAGGTTGGTTAAGGAGGGTGAGAATGTTGTTGACGGTGGTATTCAGAAGAAGGGGGATGGTAAAGTGCTGAATGCTGGCCGGGTTCTGCGGTCAAGGACTGTGGTGAATGGTGATTGTGATAAGGCGGAGAGCGGTGGTGATCAGGTGAAGAGTGATGGTAGTGAGGCGGAGAGTAATGGCGGTAAGGTGGAGAGTGGTAGTGGTAAGGCAGAGAGTGATGGTGTAAAGGCAGAGAGTGGTTGTGCGAAGGCAGAGAGTGATGTTGATAAGGCAGAGAGTGATGGTGGGAAGGCGGAGAGTGGTGGTGATGAGGCAGAGAGAGCTGCTATTAAGGCAGAGAGTAATGGTGATAAAGTGGAGAGTGATAGTGAAATGGATGAGCGTGAAAGTGTGGAAGTAGCTGAGTTTGGGAAGAATAGTAGGTTGGTTGGTAATGGTGGCGGTGTGGATAAAAAGCAGGTGAAGGAAACAGAGGTGGGTGGTAAAGCGCCAGTTGCTTGTCGGGTTCTGCGGTCACGGACTGTGGTGAATGGTGGGTGTATGGCAGAGAATGATGGTGCTCTGGTTCTTCCATGTAGACGGGATGATGGGTCTCAGAAGAATTTCCGTGATGTGATAAATGAAGGAGAGGATCAGTTGGTTACAGGGGTTACAAAGAAGTTGAAAGGTAAGCGTGGGAGACCACCCAAGGTTGAAAAAGAAGAAAGTGGGAGACCGGGTGATGGATTCAGGAAGTTGAAAGGTAAGCGTGGGAGACCTCAAGTTTCAGAAAAGGAAGAGAGTGATCTCTTGGATGGTCGGTTTAGAAAGAAGTTACGATCTGGGAAGAACAGTAGTGAGGTTTTGAAAGGAAATCTGGACAAGGAAGGAAATGTATACTTAAGAAGCAAATTGGGTTTGAAGTCACAAAGAGTGAAACCAAGTAAATTGACTAATGGTTCATATCTGGAAAGGAAGAGGATTGGGAAAGAATCGGATGTGAAGGCCTCTTCTCAAACTAAAAGGGATAAAAGAGGAAAAGGTTCAGAGAGTGTGGACAATGTGGACAGGGATGTACATAAAAAACAGAAACAGAAACAAAGCAGAGATAAACAACAGGAAAGCAAGTCGAAGGATGGCTTACGTGAGCTAAAACAGGCAGTGAGCGAAAAAATAGTTAAAATGATTCTGGCTGCAGGCTGGAAAATCGAACGTAGGCCGAGGAATGGCAAAGAGTACATGGATGCAGTGTACGTATGTCCAGCAGGAAAGACTCACTGGTCAGTAACCAAGGCTTACAATTCTCTTAAAATGAGTTGTGAAAATGTTGATCCAATGGCTTGCAAGAGCAGTTTTAAATTTACGCTGATACCACCAGAGGAACTCAGCATGCTACAGAGGGTCGGGAGCGACAAGAAGAAGAAGAAGAAGAAGGGAAAGGGTGGAGGACAGGGAAAAAATAATGCCAAGAAGAAGGCAAAGAATGGGGATACCTCTGATGGAGAGATTGAAGAGAAGAGGAGAAAGAAACTGGGTAAGTCACTGAAAGGAAAACATCTGCTTTTTGAGAAGGATGCATCAAAAAGCACAGTATGTGAGGGAAAGCTATGTTCGGTCCAGCATCACAAGCGGCAGAAGACTCAGAATAGAAAGCGATGTGCTTTGTTAGTTCGCAACTCTGAGAATGCAGATTCCGAAAATGATGGCTACATACCATATGATGGAAAGAGAACAGTACTTGCTTGGATGATTGACTTGGGAACCCTGTCACTTAATTCAAAGTTGAAGTACATGAACAAGAGGAAGAGACAAGTGCTGCTTGAGGGTAAAATTGCCAGAGATGGAATTCATTGTGGTTGCTGTGACGAAACCATCTCACTTTCAGAATTTGTAACTCATACTAGAAGTGATTACTCTGAGCCTCTTAGATATATACTTACAGACTCTGGATCTTCCCTTTTGCAATGCCTGCTAAACTCATGGAATAAACAGGAAGAGTCTGAATGCCGGGGATTCCACTCTGTAGAGGTTACTATGGAAGACCCAAATGATGATACTTGTGGGATCTGTGGAGACGGAGGAGATTTGATATGTTGTGATGGTTGTCCATCAACATTCCACAAAAGTTGCTTGGAGATAAAGAAGTTCCCATCTGGTGACTGGCACTGTGTATACTGCTCATGCAAATTCTGTGGGATGTTTGATGAGAATATGTCTGAAAGGGATGGTAGTGAGGATGTAGCTGCGTCAGTATTACTTACCTGCCATTTGTGTGAGGAAAAATATCATCAATGCTGTATTCAGGCAAAGGATGCTGTAAATGGTGATTCCAGCAGTCCATCCTTCTGTGCAAAGAATTGCCAAGAGCTATTTGAGAAACTGGAGAGCCTTCTCGGAGTTAGGCATGAAGTGGAAGAAGGGTTTTCATTGACTCTCCTTCGCCGATTTGATGTCGGTGATACACCACAGAAGGTTGATTGCAAATCAAAGCTAATTGAATGCAATGCCAAGCTGGCTGTTGCATTTTTAATTATGGATGAGTGTTTTCTGCCAATGGTTGACCACAGAAGTGGAGTCAATCTGATCCATAACATTCTTTATAATCGTGGGTCAAATTTTAACCGACTGAACTACGGTAGTTTCTTCACTGCAATTCTAGAGAGGGGTGATGAGATCATATCTGCCGCAACCATTAGGATCCATGGAAACTATTTAGCAGAGATGCCGTTTATTGGAACCCGCTATATGTATAGGCGTCAAGGAATGTGCCGCCGCCTTCTGAGTGCGATTGAATCTGCTCTCTGCTCTCTAAAAGTTGAAAGATTGGTCATACCTGCAATCTCAGAATTGACGGAAACATGGACTTCTGTTTTTGGTTTTAAGTCCCTTGAAGAATCAAGCAAGCAGAAGATGAAGAACATGAATATCTTGGTTTTCCCTGGTGTAAATATTTTACAGAAACCATTGTTGAACCAGTTAACAGAAACTAACGAGAGTCCTGTGAAAGGTTTGAGTTTCGCTGATCTCAAGCATGACGAAACCTTGAAGGATGTGGTTTGTAATACTGATGAGGGACATTTGGCTCGATCTGACGGTGAAGCACCTGCACCGTGTGTGCATGAGGGAAATGATGAGCTTGCTGCTGTTCAATCTGACACACAGAATATCAGTTCTGTATCTCATGATTATCTGGAGAGCAAGAGCAATGCTATCCCAATTCCTACTGATTCAGTGTGTGATGTTCTCGAGCTAACCAAAGAGATAAATGAATACCAAAATTCTGCTAATGCGAGGGCATTGGAATTGGTCAGCAAACAGAACCAGCCAGAGCTGTTTTGCGATACTGCTGAGGGTTTCTTGGTTGGAGCAGACATTGAGGCAACGGAATCCTTTAAACACAAGGCAAATGGTGAACTTGTTGCTGTTGAATCTGATACAATGCTTAATTGTGGTGTTTCAGATAATAATGTGGAGGGTGAAACCAATACTACGGCAATTCGTACTGGTTCAATTAGTGATGCTCATGAACAAACTGAAGATTCACAGCCTGTTTGTGGTGTTTCTCATGATAAACCAGAGGAAAACAATACTCTGACAATTCATACTGGTTCGATTACTGATGCTCATCAACAAACTGAAGAGAAAATTGAACTTCAAAATTCAGCTTCTGTTCCTGATGTTGAGGAAGTGGAATTGGGCACCGAACACAACCAACATAGCATGTCTAAAGTGGAAAGGACTTCTGATCTTCATTCTGAAGGTATTGATTCTCATGATAAACCAGAGGAAAACAATACTCTGACAATTCCTACTGGTCCAATTACTGATGCTCATCAACAAACTGAAGAGAGAACTGAACTTCAAAATTCAGCTTCTGTTCCTGATGTTGAGGAAGTGGAATTGGGCACCGAACACAACCAACATAGCATGTCTAAAGTGGAAAGGACATCTGATCTTCATTCTGAAGGTATTGATTCTGGTATAGAAGTTCTTTGTGCTTTAGGGGAGGGTACTGAAAACAGGGACAGTACGCCAATAATAGAAGATGGCATCGTTAAACCCCTTGGTCCACATGATGAAGAATATTTTCACCCGTGTACTGCAAATAATGCTACTGAACTCCAGAATGGATCTTTAGTTCATGAATCCAAAGTTTCCGGTGAAAATATTGTTGGCACTGATTCAGAGATTACATCTCAGATATCTCATGATGCAAGGGACCATAAGCATCATGATTCTCAACATTTGCAAGTTGAAGAATCTATTTCCCGTCCTGATCAAAAGATGCTTGAGACTAGTGAAGTGGAGAATAACCATTCTTGTATTCAGGACGCTACTATAGTGACGGAAGGGGTGCATCCAGAATCTGTCAAGTGTTCTGCCAGAATTCTGACCAAAACTAGCAAGGTAGCCAGTGATGAATTTCACCAGGCTCCCCCGTTGAGTCTTGAAAAAGAAGAAGATACAACTGTGCTGTTTGGCTGCAGCATTACTTGCATCTCTGGCAATGGGCAGGACACTCACAAAGTTGTATCAACTCCAGCTGATAACAATTCTCATCCTTCGTGTGGGGCTAATAAAAGTAATGGGACTTCTGTAGCCCCTGGTGTCGACTCAACTTCCTGTGGGACTAATATCGACAGTAATGGGAAATCCAAGGCCCCTGCTGTTGACTTCACTTCCAACGGGGATCATGTGAACACCAAATCCCTGCTGCAATCTGATTTTGATTCTGATGTTGATAATAACAGTCTCAGACAGCCCAACTCGAGCAATACCTGTGCATCTGGTGGGGTTAACTCATTTGGTGCTTCGGAGGTGACAGTCTTATCAAACCAGGGCCAGGCTAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010646 GO:0010648 GO:0016567 GO:0016740 GO:0017015 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0023051 GO:0023057 GO:0030510 GO:0030514 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045892 GO:0045934 GO:0046332 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0070013 GO:0070410 GO:0070412 GO:0070647 GO:0071704 GO:0080090 GO:0090092 GO:0090101 GO:0090287 GO:0090288 GO:0140096 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1903844 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AT_hook Jas PHD

Protein Analysis

1739

Amino Acids

189.65

Weight (kDa)

5.64

Isoelectric Point (pI)

46.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7028	PF22970	500 - 559	3.7e-10	Domain of unknown function (DUF7028)
TDBD	PF16135	730 - 778	7.2e-14	Tify domain binding domain
IDM1_C	PF23209	970 - 1109	1.1e-58	Increased DNA methylation 1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0002658)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36670 AT5G36670 AT5G36670 AT5G36670 AT5G36670 AT5G36740 AT5G36740 AT5G36740 AT5G36740 AT5G36740
fragaria_vesca	FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330 FvH4_2g23330
malus_domestica	MD03G1262700.v1.1 MD11G1283400.v1.1
prunus_persica	Prupe.8G239000_v2.0.a1
pyrus_communis	pycom03g21020 pycom11g25090
rosa_chinensis	RchiOBHm_Chr6g0290741 RchiOBHm_Chr6g0290751
rosa_laevigata	RLG00000012182
rosa_multiflora	Rmu_co8421285.1_g000001 Rmu_sc0000043.1_g000020
rosa_roxburghii	Rroxscaffold_7G00176760
rosa_rugosa	Rorug06G0214400 Rorug06G0214500 Rorug06G0214600
rosa_samantha	Rh6AG325000 Rh6BG332600 Rh6CG338300 Rh6DG325800
rosa_wichuraiana	Rw6G028220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2964
AarI	CACCTGC	2 cut(s)	832, 3475
AasI	GACNNNNNNGTC	1 cut(s)	1642
Acc36I	ACCTGC	5 cut(s)	12, 832, 2633, 3205, 3475
AccB1I	GGYRCC	2 cut(s)	4061, 4268
AccB7I	CCANNNNNTGG	1 cut(s)	2084
AccBSI	CCGCTC	1 cut(s)	516
AccI	GTMKAC	4 cut(s)	961, 1260, 2535, 4974
AclWI	GGATC	9 cut(s)	1023, 1688, 2320, 2435, 2941, 3055, 3068, 4501, 5061
AcoI	YGGCCR	1 cut(s)	460
AcuI	CTGAAG	5 cut(s)	3918, 4020, 4140, 4227, 4347
AcyI	GRCGYC	1 cut(s)	3118
AdeI	CACNNNGTG	1 cut(s)	3476
AfaI	GTAC	9 cut(s)	1440, 1601, 1616, 2100, 2153, 3865, 4375, 4393, 4462
AfiI	CCNNNNNNNGG	9 cut(s)	134, 1106, 1589, 2084, 3095, 3368, 3917, 4931, 5206
AflII	CTTAAG	1 cut(s)	1264
AhlI	ACTAGT	1 cut(s)	4658
AjnI	CCWGG	5 cut(s)	3301, 4791, 4966, 5199, 5205
AjuI	GAANNNNNNNTTGG	4 cut(s)	791, 823, 4518, 4550
AleI	CACNNNNGTG	1 cut(s)	2931
AloI	GAACNNNNNNTCC	4 cut(s)	4485, 4517, 5054, 5086
Alw21I	GWGCWC	1 cut(s)	946
Alw26I	GTCTC	8 cut(s)	981, 1057, 1090, 1132, 2164, 2430, 4649, 5120
AlwI	GGATC	9 cut(s)	1023, 1688, 2320, 2435, 2941, 3055, 3068, 4501, 5061
AlwNI	CAGNNNCTG	4 cut(s)	2309, 4031, 4238, 4730
Ama87I	CYCGRG	2 cut(s)	3608, 5131
AoxI	GGCC	5 cut(s)	460, 1368, 1580, 5022, 5203
ApeKI	GCWGC	8 cut(s)	707, 1556, 2179, 2606, 3503, 4845, 4848, 5077
Asp700I	GAANNNNTTC	3 cut(s)	986, 1405, 4449
AspLEI	GCGC	1 cut(s)	874
AspS9I	GGNCC	6 cut(s)	1978, 4178, 4430, 4582, 5023, 5203
AsuC2I	CCSGG	3 cut(s)	464, 1101, 2372
AvaI	CYCGRG	2 cut(s)	3608, 5131
AvaII	GGWCC	4 cut(s)	1978, 4178, 4430, 4582
BaeGI	GKGCMC	2 cut(s)	4064, 4271
BaeI	ACNNNNGTAYC	2 cut(s)	4818, 4851
BamHI	GGATCC	1 cut(s)	3060
BanI	GGYRCC	2 cut(s)	4061, 4268
BarI	GAAGNNNNNNTAC	2 cut(s)	4939, 4971
BbsI	GAAGAC	2 cut(s)	2006, 2409
Bbv12I	GWGCWC	1 cut(s)	946
BbvCI	CCTCAGC	1 cut(s)	3711
BbvI	GCAGC	8 cut(s)	694, 1543, 2166, 2593, 3490, 4832, 4860, 5064
BceAI	ACGGC	2 cut(s)	3073, 3870
BciT130I	CCWGG	5 cut(s)	3303, 4793, 4968, 5201, 5207
BciVI	GTATCC	1 cut(s)	1846
BclI	TGATCA	2 cut(s)	523, 4639
BcnI	CCSGG	3 cut(s)	464, 1101, 2372
BcoDI	GTCTC	8 cut(s)	981, 1057, 1090, 1132, 2164, 2430, 4649, 5120
BcuI	ACTAGT	1 cut(s)	4658
BfaI	CTAG	5 cut(s)	2265, 3020, 4659, 4764, 5211
BfmI	CTRYAG	6 cut(s)	1557, 1757, 2385, 4701, 4846, 4959
BfoI	RGCGCY	1 cut(s)	875
BfrI	CTTAAG	1 cut(s)	1264
BfuAI	ACCTGC	5 cut(s)	12, 832, 2633, 3205, 3475
BfuI	GTATCC	1 cut(s)	1846
BglI	GCCNNNNNGGC	1 cut(s)	2876
BglII	AGATCT	1 cut(s)	14
BmcAI	AGTACT	1 cut(s)	2100
Bme1390I	CCNGG	8 cut(s)	464, 1101, 2372, 3303, 4793, 4968, 5201, 5207
Bme18I	GGWCC	4 cut(s)	1978, 4178, 4430, 4582
BmeT110I	CYCGRG	2 cut(s)	3608, 5131
BmgT120I	GGNCC	6 cut(s)	1978, 4178, 4430, 4582, 5023, 5203
BmiI	GGNNCC	8 cut(s)	2125, 3062, 3100, 4063, 4270, 4583, 4797, 5025
BmrFI	CCNGG	8 cut(s)	464, 1101, 2372, 3303, 4793, 4968, 5201, 5207
BmrI	ACTGGG	1 cut(s)	1904
BmuI	ACTGGG	1 cut(s)	1904
BpiI	GAAGAC	2 cut(s)	2006, 2409
BpmI	CTGGAG	4 cut(s)	2765, 3575, 4469, 4887
Bpu10I	CCTNAGC	1 cut(s)	3711
BpuEI	CTTGAG	4 cut(s)	1128, 2204, 3374, 4673
BpuMI	CCSGG	3 cut(s)	464, 1101, 2372
BsaAI	YACGTR	2 cut(s)	1508, 1618
BsaBI	GATNNNNATC	2 cut(s)	3987, 4194
BsaHI	GRCGYC	1 cut(s)	3118
BsaI	GGTCTC	4 cut(s)	981, 1057, 1090, 1132
BsaWI	WCCGGW	1 cut(s)	4520
BsaXI	ACNNNNNCTCC	4 cut(s)	177, 207, 1087, 1117
Bsc4I	CCNNNNNNNGG	9 cut(s)	134, 1106, 1589, 2084, 3095, 3368, 3917, 4931, 5206
Bse3DI	GCAATG	4 cut(s)	2333, 2872, 3574, 4876
Bse8I	GATNNNNATC	2 cut(s)	3987, 4194
BseBI	CCWGG	5 cut(s)	3303, 4793, 4968, 5201, 5207
BseJI	GATNNNNATC	2 cut(s)	3987, 4194
BseLI	CCNNNNNNNGG	9 cut(s)	134, 1106, 1589, 2084, 3095, 3368, 3917, 4931, 5206
BseMI	GCAATG	4 cut(s)	2333, 2872, 3574, 4876
BseRI	GAGGAG	6 cut(s)	198, 217, 220, 310, 1897, 2457
BseSI	GKGCMC	2 cut(s)	4064, 4271
BseXI	GCAGC	8 cut(s)	694, 1543, 2166, 2593, 3490, 4832, 4860, 5064
BsgI	GTGCAG	1 cut(s)	3453
Bsh1285I	CGRYCG	1 cut(s)	293
BshFI	GGCC	5 cut(s)	462, 1370, 1582, 5024, 5205
BshNI	GGYRCC	2 cut(s)	4061, 4268
BsiEI	CGRYCG	1 cut(s)	293
BsiHKAI	GWGCWC	1 cut(s)	946
BsiHKCI	CYCGRG	2 cut(s)	3608, 5131
BsiSI	CCGG	5 cut(s)	83, 463, 1100, 2371, 4521
BslFI	GGGAC	7 cut(s)	69, 3232, 3450, 4400, 4595, 4966, 5005
BslI	CCNNNNNNNGG	9 cut(s)	134, 1106, 1589, 2084, 3095, 3368, 3917, 4931, 5206
BsmAI	GTCTC	8 cut(s)	981, 1057, 1090, 1132, 2164, 2430, 4649, 5120
BsmBI	CGTCTC	1 cut(s)	2430
BsmFI	GGGAC	7 cut(s)	69, 3232, 3450, 4400, 4595, 4966, 5005
BsmI	GAATGC	4 cut(s)	460, 2053, 2372, 2867
BsnI	GGCC	5 cut(s)	462, 1370, 1582, 5024, 5205
Bso31I	GGTCTC	4 cut(s)	981, 1057, 1090, 1132
BsoBI	CYCGRG	2 cut(s)	3608, 5131
Bsp1286I	GDGCHC	3 cut(s)	946, 4064, 4271
Bsp1407I	TGTACA	1 cut(s)	1438
Bsp19I	CCATGG	1 cut(s)	3064
BspANI	GGCC	5 cut(s)	462, 1370, 1582, 5024, 5205
BspHI	TCATGA	7 cut(s)	3544, 3886, 3928, 4135, 4504, 4570, 4594
BspLI	GGNNCC	8 cut(s)	2125, 3062, 3100, 4063, 4270, 4583, 4797, 5025
BspMAI	CTGCAG	2 cut(s)	1561, 4850
BspMI	ACCTGC	5 cut(s)	12, 832, 2633, 3205, 3475
BspPI	GGATC	9 cut(s)	1023, 1688, 2320, 2435, 2941, 3055, 3068, 4501, 5061
BspT107I	GGYRCC	2 cut(s)	4061, 4268
BspTI	CTTAAG	1 cut(s)	1264
BspTNI	GGTCTC	4 cut(s)	981, 1057, 1090, 1132
BsrBI	CCGCTC	1 cut(s)	516
BsrDI	GCAATG	4 cut(s)	2333, 2872, 3574, 4876
BsrGI	TGTACA	1 cut(s)	1438
BssNAI	GTATAC	2 cut(s)	1261, 2536
BssNI	GRCGYC	1 cut(s)	3118
BssT1I	CCWWGG	5 cut(s)	1071, 1653, 3064, 4426, 5019
Bst1107I	GTATAC	2 cut(s)	1261, 2536
Bst2UI	CCWGG	5 cut(s)	3303, 4793, 4968, 5201, 5207
BstACI	GRCGYC	1 cut(s)	3118
BstAFI	CTTAAG	1 cut(s)	1264
BstAPI	GCANNNNNTGC	4 cut(s)	2332, 2675, 4472, 4857
BstAUI	TGTACA	1 cut(s)	1438
BstBAI	YACGTR	2 cut(s)	1508, 1618
BstC8I	GCNNGC	8 cut(s)	460, 1561, 1705, 1754, 2333, 2877, 3266, 3501
BstDSI	CCRYGG	1 cut(s)	3064
BstENI	CCTNNNNNAGG	1 cut(s)	3366
BstH2I	RGCGCY	1 cut(s)	875
BstHHI	GCGC	1 cut(s)	874
BstMAI	GTCTC	8 cut(s)	981, 1057, 1090, 1132, 2164, 2430, 4649, 5120
BstMCI	CGRYCG	1 cut(s)	293
BstNI	CCWGG	5 cut(s)	3303, 4793, 4968, 5201, 5207
BstNSI	RCATGY	3 cut(s)	1756, 4087, 4294
BstSCI	CCNGG	8 cut(s)	462, 1099, 2370, 3301, 4791, 4966, 5199, 5205
BstSFI	CTRYAG	6 cut(s)	1557, 1757, 2385, 4701, 4846, 4959
BstSLI	GKGCMC	2 cut(s)	4064, 4271
BstSNI	TACGTA	1 cut(s)	1618
BstV1I	GCAGC	8 cut(s)	694, 1543, 2166, 2593, 3490, 4832, 4860, 5064
BstV2I	GAAGAC	2 cut(s)	2006, 2409
BstX2I	RGATCY	5 cut(s)	14, 2312, 2427, 3060, 4493
BstXI	CCANNNNNNTGG	1 cut(s)	2933
BstYI	RGATCY	5 cut(s)	14, 2312, 2427, 3060, 4493
BstZ17I	GTATAC	2 cut(s)	1261, 2536
BsuI	GTATCC	1 cut(s)	1846
BsuRI	GGCC	5 cut(s)	462, 1370, 1582, 5024, 5205
BtgI	CCRYGG	1 cut(s)	3064
BtgZI	GCGATG	1 cut(s)	2032
BtsI	GCAGTG	3 cut(s)	1530, 1617, 3009
BveI	ACCTGC	5 cut(s)	12, 832, 2633, 3205, 3475
Cac8I	GCNNGC	8 cut(s)	460, 1561, 1705, 1754, 2333, 2877, 3266, 3501
CaiI	CAGNNNCTG	4 cut(s)	2309, 4031, 4238, 4730
CciI	TCATGA	7 cut(s)	3544, 3886, 3928, 4135, 4504, 4570, 4594
CfoI	GCGC	1 cut(s)	874
Cfr13I	GGNCC	6 cut(s)	1978, 4178, 4430, 4582, 5023, 5203
CseI	GACGC	3 cut(s)	2598, 3107, 4703
Csp6I	GTAC	9 cut(s)	1439, 1600, 1615, 2099, 2152, 3864, 4374, 4392, 4461
CspCI	CAANNNNNGTGG	6 cut(s)	465, 500, 2471, 2506, 2536, 2571
CviQI	GTAC	9 cut(s)	1439, 1600, 1615, 2099, 2152, 3864, 4374, 4392, 4461
DraI	TTTAAA	2 cut(s)	1720, 3757
DraIII	CACNNNGTG	1 cut(s)	3476
DrdI	GACNNNNNNGTC	1 cut(s)	1642
DseDI	GACNNNNNNGTC	1 cut(s)	1642
EaeI	YGGCCR	1 cut(s)	460
EciI	GGCGGA	3 cut(s)	524, 566, 692
Eco105I	TACGTA	1 cut(s)	1618
Eco130I	CCWWGG	5 cut(s)	1071, 1653, 3064, 4426, 5019
Eco147I	AGGCCT	1 cut(s)	1370
Eco31I	GGTCTC	4 cut(s)	981, 1057, 1090, 1132
Eco32I	GATATC	1 cut(s)	4566
Eco47I	GGWCC	4 cut(s)	1978, 4178, 4430, 4582
Eco57I	CTGAAG	5 cut(s)	3918, 4020, 4140, 4227, 4347
Eco88I	CYCGRG	2 cut(s)	3608, 5131
EcoNI	CCTNNNNNAGG	1 cut(s)	3366
EcoO109I	RGGNCCY	1 cut(s)	5023
EcoRI	GAATTC	2 cut(s)	2208, 4749
EcoRII	CCWGG	5 cut(s)	3301, 4791, 4966, 5199, 5205
EcoRV	GATATC	1 cut(s)	4566
EcoT14I	CCWWGG	5 cut(s)	1071, 1653, 3064, 4426, 5019
EcoT22I	ATGCAT	1 cut(s)	1942
ErhI	CCWWGG	5 cut(s)	1071, 1653, 3064, 4426, 5019
Esp3I	CGTCTC	1 cut(s)	2430
FalI	AAGNNNNNCTT	4 cut(s)	1487, 1519, 2166, 2198
FaqI	GGGAC	7 cut(s)	69, 3232, 3450, 4400, 4595, 4966, 5005
FauI	CCCGC	1 cut(s)	3110
FauNDI	CATATG	1 cut(s)	2080
FbaI	TGATCA	2 cut(s)	523, 4639
FblI	GTMKAC	4 cut(s)	961, 1260, 2535, 4974
FspBI	CTAG	5 cut(s)	2265, 3020, 4659, 4764, 5211
GlaI	GCGC	1 cut(s)	873
GsuI	CTGGAG	4 cut(s)	2765, 3575, 4469, 4887
HaeII	RGCGCY	1 cut(s)	875
HaeIII	GGCC	5 cut(s)	462, 1370, 1582, 5024, 5205
HapII	CCGG	5 cut(s)	83, 463, 1100, 2371, 4521
HgaI	GACGC	3 cut(s)	2598, 3107, 4703
HhaI	GCGC	1 cut(s)	874
Hin1I	GRCGYC	1 cut(s)	3118
Hin6I	GCGC	1 cut(s)	872
HinP1I	GCGC	1 cut(s)	872
HincII	GTYRAC	6 cut(s)	415, 2923, 3342, 4975, 5035, 5161
HindII	GTYRAC	6 cut(s)	415, 2923, 3342, 4975, 5035, 5161
HpaI	GTTAAC	2 cut(s)	3342, 5161
HpaII	CCGG	5 cut(s)	83, 463, 1100, 2371, 4521
HpyCH4IV	ACGT	3 cut(s)	1507, 1576, 1617
HpySE526I	ACGT	3 cut(s)	1507, 1576, 1617
Hsp92I	GRCGYC	1 cut(s)	3118
HspAI	GCGC	1 cut(s)	872
Ksp22I	TGATCA	2 cut(s)	523, 4639
KspAI	GTTAAC	2 cut(s)	3342, 5161
LmnI	GCTCC	3 cut(s)	1770, 3728, 4801
Lsp1109I	GCAGC	8 cut(s)	694, 1543, 2166, 2593, 3490, 4832, 4860, 5064
MaeI	CTAG	5 cut(s)	2265, 3020, 4659, 4764, 5211
MaeII	ACGT	3 cut(s)	1507, 1576, 1617
MbiI	CCGCTC	1 cut(s)	516
MflI	RGATCY	5 cut(s)	14, 2312, 2427, 3060, 4493
MhlI	GDGCHC	3 cut(s)	946, 4064, 4271
MlyI	GAGTC	9 cut(s)	1630, 1996, 2300, 2369, 2788, 2946, 3367, 4816, 4970
MmeI	TCCRAC	2 cut(s)	3706, 5070
Mph1103I	ATGCAT	1 cut(s)	1942
MroXI	GAANNNNTTC	3 cut(s)	986, 1405, 4449
MslI	CAYNNNNRTG	2 cut(s)	2634, 2931
MspA1I	CMGCKG	1 cut(s)	4907
MspCI	CTTAAG	1 cut(s)	1264
MspI	CCGG	5 cut(s)	83, 463, 1100, 2371, 4521
MspR9I	CCNGG	8 cut(s)	464, 1101, 2372, 3303, 4793, 4968, 5201, 5207
Mva1269I	GAATGC	4 cut(s)	460, 2053, 2372, 2867
MvaI	CCWGG	5 cut(s)	3303, 4793, 4968, 5201, 5207
NciI	CCSGG	3 cut(s)	464, 1101, 2372
NcoI	CCATGG	1 cut(s)	3064
NdeI	CATATG	1 cut(s)	2080
NlaIV	GGNNCC	8 cut(s)	2125, 3062, 3100, 4063, 4270, 4583, 4797, 5025
NmeAIII	GCCGAG	1 cut(s)	1608
NsiI	ATGCAT	1 cut(s)	1942
NspI	RCATGY	3 cut(s)	1756, 4087, 4294
OliI	CACNNNNGTG	1 cut(s)	2931
PaeI	GCATGC	1 cut(s)	1756
PaeR7I	CTCGAG	2 cut(s)	3608, 5131
PagI	TCATGA	7 cut(s)	3544, 3886, 3928, 4135, 4504, 4570, 4594
PaqCI	CACCTGC	2 cut(s)	832, 3475
PceI	AGGCCT	1 cut(s)	1370
PctI	GAATGC	4 cut(s)	460, 2053, 2372, 2867
PdmI	GAANNNNTTC	3 cut(s)	986, 1405, 4449
PflMI	CCANNNNNTGG	1 cut(s)	2084
PleI	GAGTC	9 cut(s)	1630, 1996, 2300, 2368, 2788, 2945, 3366, 4815, 4970
PpsI	GAGTC	9 cut(s)	1630, 1996, 2300, 2368, 2788, 2945, 3366, 4815, 4970
Ppu21I	YACGTR	2 cut(s)	1508, 1618
PsiI	TTATAA	1 cut(s)	2964
Psp6I	CCWGG	5 cut(s)	3301, 4791, 4966, 5199, 5205
PspGI	CCWGG	5 cut(s)	3301, 4791, 4966, 5199, 5205
PspN4I	GGNNCC	8 cut(s)	2125, 3062, 3100, 4063, 4270, 4583, 4797, 5025
PspPI	GGNCC	6 cut(s)	1978, 4178, 4430, 4582, 5023, 5203
PspXI	VCTCGAGB	1 cut(s)	5131
PstI	CTGCAG	2 cut(s)	1561, 4850
PstNI	CAGNNNCTG	4 cut(s)	2309, 4031, 4238, 4730
PsuI	RGATCY	5 cut(s)	14, 2312, 2427, 3060, 4493
PvuII	CAGCTG	1 cut(s)	4907
RsaI	GTAC	9 cut(s)	1440, 1601, 1616, 2100, 2153, 3865, 4375, 4393, 4462
RsaNI	GTAC	9 cut(s)	1439, 1600, 1615, 2099, 2152, 3864, 4374, 4392, 4461
RseI	CAYNNNNRTG	2 cut(s)	2634, 2931
SalI	GTCGAC	1 cut(s)	4973
Sau96I	GGNCC	6 cut(s)	1978, 4178, 4430, 4582, 5023, 5203
ScaI	AGTACT	1 cut(s)	2100
SchI	GAGTC	9 cut(s)	1630, 1996, 2300, 2369, 2788, 2946, 3367, 4816, 4970
ScrFI	CCNGG	8 cut(s)	464, 1101, 2372, 3303, 4793, 4968, 5201, 5207
SduI	GDGCHC	3 cut(s)	946, 4064, 4271
SfcI	CTRYAG	6 cut(s)	1557, 1757, 2385, 4701, 4846, 4959
Sfr274I	CTCGAG	2 cut(s)	3608, 5131
SinI	GGWCC	4 cut(s)	1978, 4178, 4430, 4582
SlaI	CTCGAG	2 cut(s)	3608, 5131
SmiMI	CAYNNNNRTG	2 cut(s)	2634, 2931
SmlI	CTYRAG	7 cut(s)	1143, 1264, 2183, 3389, 3608, 4652, 5131
SmoI	CTYRAG	7 cut(s)	1143, 1264, 2183, 3389, 3608, 4652, 5131
SnaBI	TACGTA	1 cut(s)	1618
SpeI	ACTAGT	1 cut(s)	4658
SphI	GCATGC	1 cut(s)	1756
SseBI	AGGCCT	1 cut(s)	1370
SspI	AATATT	3 cut(s)	3313, 4448, 4531
SspMI	CTAG	5 cut(s)	2265, 3020, 4659, 4764, 5211
StuI	AGGCCT	1 cut(s)	1370
StyD4I	CCNGG	8 cut(s)	462, 1099, 2370, 3301, 4791, 4966, 5199, 5205
StyI	CCWWGG	5 cut(s)	1071, 1653, 3064, 4426, 5019
TaiI	ACGT	3 cut(s)	1510, 1579, 1620
TaqI	TCGA	9 cut(s)	293, 1493, 1572, 3448, 3609, 3974, 4974, 5001, 5132
TaqII	GACCGA	1 cut(s)	1966
TatI	WGTACW	5 cut(s)	1438, 1599, 2098, 2151, 4460
TauI	GCSGC	4 cut(s)	1996, 3050, 3135, 3138
TseI	GCWGC	8 cut(s)	707, 1556, 2179, 2606, 3503, 4845, 4848, 5077
TspGWI	ACGGA	6 cut(s)	916, 980, 2454, 3230, 3761, 4724
Van91I	CCANNNNNTGG	1 cut(s)	2084
Vha464I	CTTAAG	1 cut(s)	1264
VpaK11BI	GGWCC	4 cut(s)	1978, 4178, 4430, 4582
XagI	CCTNNNNNAGG	1 cut(s)	3366
XbaI	TCTAGA	1 cut(s)	3019
XceI	RCATGY	3 cut(s)	1756, 4087, 4294
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2520
XhoI	CTCGAG	2 cut(s)	3608, 5131
XmiI	GTMKAC	4 cut(s)	961, 1260, 2535, 4974
XmnI	GAANNNNTTC	3 cut(s)	986, 1405, 4449
XspI	CTAG	5 cut(s)	2265, 3020, 4659, 4764, 5211
ZrmI	AGTACT	1 cut(s)	2100
Zsp2I	ATGCAT	1 cut(s)	1942

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.