FvH4_2g23910

HUA2-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005320
Physical Location & Seq
Reverse (-)
19535589 .. 19546910
11322 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g23910.t3

Sequence Viewer

Length: 4317 bp
ATGGCGCCTAGCCGGAGGAAAGGAGCTAGCAAAGCCGCTCAGGCCGCCGCCGCTCGCCGCCAGTGGAAGGTCGGCGATCTTGTGCTTGCTAAAGTCAAAGGCTTCCCGGCTTGGCCTGCTACGGTAAGTGAGCCGGAGAAGTGGGGTTACTCTGCTGATTGGAAGAAAGTACTCGTTTTCTTCTTTGGAACACAACAGATTGCTTTCTGCAACCCTGCTGATGTTGAGGCATTTACTGAGGAGAAGAAACAGTCTCTTCTTGGCAAGCGTCATGGGAAGGGTGCTGATTTTGTTCGTGCAGTCCAGGAGATTATTGATAGTTTTGAGAAGTTGAAGAAGGAGGACCAAGTTGATGAGTTCAACTCTAGTGCAAATGGTGGGAACTCAGTTGACTCTTCATGCAATTTTGGTTCAAAGGATCAGAAAGAAGCTCCTGAAGCTATTCTTGATTTACATCCAAAATCTTCATCTTCTACCATTGATAAAAATGAACCAAGTAACTCTGTTGAGGATGCTTCAGCTACTGCACTAGTAGATGCCACCCTTGATAAGGAGGACTTGATAGAGGAGCCTGCTGCTACAACAATGGTTTCTGAAACACCTGTGCCAACAACATGCTCTTCCAAAAAAAGATCTGGAGAGTTGCGATTACAGAGCTGCGTCTCAAAAAGTGAGGAAGCACCAGCTCGTAGGTCAAGAAGCTCATCCAGGACGGAATTACGTAGATTAGGGAGCTTCATAATGCCATGTGATGATGATGCCAAGAATGCTGGGTATGTATCTGCTAATGCAGTTCAGGACAGAAGCTTAAGAAGGAATAAACGAACACGGAAATCACCTGATGCCTCCGTGTGTGATAATGTTAAATTGGCAGCTTCTGTTTCAAATGGTTGTGTTGAAGACGATGGTTCTGAAGTTGTTCCAGTTGATTCTGGCACATTTAGTTTGAACGACGGCAGTGTTATTGATTCTGGTTGCAAAGGAGAACACTCGGACGCTGTTGCTGAATGCATGGAAGGAGATGCTGAGTTGATTAAAGGACTTGATCTTCAAATAAAGGCTGTTAGTAAGAAGAAAAGGAAACCAAACAGAAAGAGAGGTACTAATGATGCAGCTGAGCCTATTGCTATATTGGACAAGGAGACGGTTCAAGAAGTTAATTTGCAAAGCAGTAGTCAAAGCATGCAGACTGATTGTGGAAATATGAATGGAAATTTTTCCAAGGAAGATGGAGATGAGCACCTGCCGTTGGTGAAACGAGCCAGGGTTCGTATGAATAAACCATCTTCTGTTGAGGAAGTTGATAGCTCTTCACATATTGAAGAAAGTCTGAAGGAAGTCATGCTCATTCCATCAGGGTTGATCAGCACATCTCCAATATGTGATGACATTTGCCCTAGTGGTAGGGACTCATTTGTGGTAAATGTATCTCTAGATAATACTACACCTTCAAGAGTTGGCACTCAAAGTTTGGAAAATAGGCCTCAGCTTTGGAACTCGAAGAAAGACCAGTCTTTTGGTGGTTTGGCTGATGGTGAAGCTGTTTTACCTCCATCTAAGCGTCTTCATCGTGCACTAGAAGCCATGTCAGCTAATGCAGCTGAGGATGATGAAAGATGTAACTATGATTCATCAGCCATGAGGACATCTACTATTGATTGTAATAATTCTTCCGGGAACAAATCCGTTACTATAAATGTGGAAAGTTATTCAGGGAATGGTCTGGGACTGCATAGTGAAGACTCTTTTGGCAATAATGCTTCGGGGTCCTCTACCAGTCCAAACCGAGTAATCCTGGAGGAAAATACTAAGTCAGCAATGGAAGTGGATGTATGTGATCAAAGAAGGAATAGTCCTGACACTCGGAACAATCAATCCGTTAATGGTTTTCCAGATTCTGGGAACCGTTCTAGTGGTGAAATTCTTTCTGCTGGTTCTACCGGCTGTTGTGCTATTGGAACTGCAGTTCAGACCAGAAGTCTAGGGAATTTATCACCTAGTATGGAGAGAAGAGATGCTGGTACTGAATGTAATCAAGGTTCAATGGTAGAGTGTCCTCAAAAAGATGAAGGTCATGCAAAATTTGAATCAAGTAATAATGCAGAAAATCTTGGTACTGATTGTGAGAAAATTGAATCAAGGATTAAAGATGAAATTGGTGATACTAATTGCGATACTTTTGAGCATACGTTGAAGAGCTTAGATCCTGTACCAGGCACCAGCCATGGATTTGTTGAAGTTCCACATTGTGTTGATGCTAGTCCACTTCACTGTGGTGCAGAGAGCCCTAGGGAAAAAATCAAGTGTCTGGATCCTCAGTCACAAGAGAACAAAGACGTAAAGGACATGTTGGATGCTCTAAAAGAGGTCAAGCATACACACATAGAGAAAGATCCTAGTTCAGTTTCCTGTCCAAATGAGTACTTGACTGAAAAACATGTTGCTGGTGTCCGGTCAAGTCCTAACTTAACAGATGGAGGAGATTCTCTTGCACAAGCATCACCTCCTAATACTTCAGCCTGTCGTATTTCTACTTCTGATAGTAGTAATATTCTTCAGGACAACGGCAGTTGTAGTCCGGATGTTGATTTGCAGCACAAGAGAACATCTACTCCACCAGTTGATGAGGATGAAAGGTCAGAAGCAGTGGTTTGTCAGAGATCAAAGTCTGTGAGCAGGTATGCAGAAGCACTTGCTGCTCTGTCATCCTTTGAGACAATTCTAGGAACCTTGACAAGGACCAAGGACAGCATTGGTCGAGCAACTCGTGTAGCTATTGATTGTGGAAAGATTGGTGTGGCTTCTAAGGTGTTGGAAATTTTGGCACGTCATCTGGAAAATGAGTCGAGCTTACACCGTAGGATCGATTTCTTTTTCCTGGTGGATTCAATTGCTCAGCATTCTCGAGGTGTGAAAGGTGATATTGGTGGTATGTTTTCTTCTGCTATTCAAGCAGTGCTACCACGGTTATTGTCAGCTGCTGCTCCTCCTGGAAGTTCAGCTAATGAGAATCGTAGGCAGTGTCTAAAGGTTTTGAAACTTTGGTTGGAGAGAAGGATTGTCCCCGAGTCCATAATCCGTCGCCATATGCGAGAACTTGATACTATTGGTGGCTCTTCTGCTGGTGCCTATTGCCGGCGCTCCTCTAGAACAGAAAGGTCTTTGGATGATCCTCTTAGAGAGATGGAGGGTATGCTTGTCGATGAATACGGAAGCAATTCAAGTTTTCAACTTCCGGGATTCTGTATGCCCCGCATGCTCAAGGATGAAAATGGTGGAAGTGATTCTGATGGGGAAATTTTTGAGGCTGTCACCCCTGAACATAACCCTCTAACTAATGAAGAACATGAAGTCGCTCCTGCAACTGAAAGGCATAGGCATATCTTGGAAGACGTTGATGGAGAACTTGAAATGGAGGATGTGGCTCCGTCTTGTGATGTTGACATGAGTTCATCTTGCGGTGTTGCTGGAGTCAATGTTGTGCAGGCTTCACATAATCAGTTTGAACAACATTTTTCACATCCCTTTGCCCCTCCACTACCTCAAGATGTGCCTCCCTCATCTCCTCCACTACCATCGTCTCCTCCGCCCCCACCAGCAGCGGCTCCTCTGCCCCCACCTCATGTCATTCACCCTCCATGTGCTACGTCTGATTTGAATCCATACACTGACTCACATAATGTGCATGACAGCAGAGTTCCACCTCCGCCTCTGCAGTTGAATGGGCCAAGAATTAACCAAGCAATTCCTGATGCAGTGCACTATCATGGCGCTGAATCTAGAGATCTTCACAGGCAGATGCCTGATTCTACTTCCTGCTCGTATAGTAGTTTTCCTTCAAATTCAGGGAGAAATATTCCACAAACTGATGGGCCTACATTCCATGGCAAAGGTTACCCTTTACGGCCACCTCATGCTCCACCGTCAAATCAGTTTTCTTATGTAAAAGGAGACCATCATGTAAAGCCACGGCGTGAAGTCCCGCCGCCTTATCACAACAGATTTGACTTCATGCAGAATGGGGACCGAGAACACTATTACAATAATCATGAAAGAATGAAACCAGCTCCTTATGAGCCCCGTGAGAACTGGAGGTTTCCTGCACATTCTTATTCTGGTCCTCGCTATCCTGAAAAGGGCAAGGCGTCTTATGGAAATGCTCCGTTTGCTGGCCCCCCTCGTGGACCAACAAGATTACCTGGTCATGGGTGGAGATTTCCTCCACGGTCAGCAAACCACAGACACTCTTTTATACCGCCTTATGATGGTCCGATACCTGTCACCAACAGAGGTCCAGGATTTTGGCGGCCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CTD_bind GSHPx PWWP

Protein Analysis

1439

Amino Acids

156.54

Weight (kDa)

5.73

Isoelectric Point (pI)

61.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CID PF04818 839 - 963 1.6e-14 CID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1251
Acc36I ACCTGC 2 cut(s) 1251, 2667
AccB1I GGYRCC 3 cut(s) 4, 2216, 3125
AccB7I CCANNNNNTGG 1 cut(s) 1898
AccBSI CCGCTC 2 cut(s) 38, 53
AccIII TCCGGA 1 cut(s) 2578
AclWI GGATC 7 cut(s) 426, 2198, 2306, 2319, 2387, 2870, 3164
AcoI YGGCCR 1 cut(s) 3905
AcsI RAATTY 7 cut(s) 1213, 1920, 1987, 2081, 2817, 3297, 3841
AcuI CTGAAG 6 cut(s) 456, 501, 933, 1352, 2499, 2540
AcyI GRCGYC 2 cut(s) 5, 4145
AdeI CACNNNGTG 2 cut(s) 2249, 3974
AfaI GTAC 6 cut(s) 171, 1102, 2023, 2116, 2211, 2423
AflII CTTAAG 1 cut(s) 808
AflIII ACRYGT 2 cut(s) 2346, 2437
AhdI GACNNNNNGTC 1 cut(s) 1977
AhlI ACTAGT 1 cut(s) 529
AjiI CACGTC 1 cut(s) 2828
AjnI CCWGG 9 cut(s) 303, 707, 1262, 1794, 2212, 2877, 2989, 4198, 4294
AjuI GAANNNNNNNTTGG 4 cut(s) 1731, 1763, 3029, 3061
AleI CACNNNNGTG 1 cut(s) 2274
AloI GAACNNNNNNTCC 6 cut(s) 1859, 1891, 1950, 1982, 2596, 2628
Alw21I GWGCWC 3 cut(s) 1242, 1576, 3762
Alw26I GTCTC 6 cut(s) 258, 667, 1136, 2708, 3585, 3945
Alw44I GTGCAC 2 cut(s) 1572, 3758
AlwI GGATC 7 cut(s) 426, 2198, 2306, 2319, 2387, 2870, 3164
AlwNI CAGNNNCTG 4 cut(s) 524, 878, 1898, 2981
Ama87I CYCGRG 2 cut(s) 2904, 3065
Aor13HI TCCGGA 1 cut(s) 2578
AoxI GGCC 8 cut(s) 42, 113, 1481, 3725, 3872, 3905, 4171, 4307
ApaLI GTGCAC 2 cut(s) 1572, 3758
ApoI RAATTY 7 cut(s) 1213, 1920, 1987, 2081, 2817, 3297, 3841
ArsI GACNNNNNNTTYG 4 cut(s) 1159, 1191, 1499, 1531
Asp700I GAANNNNTTC 5 cut(s) 441, 918, 1216, 3217, 3283
AspA2I CCTAGG 1 cut(s) 2288
AspLEI GCGC 3 cut(s) 7, 3141, 3773
AsuC2I CCSGG 3 cut(s) 107, 1675, 3237
AsuNHI GCTAGC 1 cut(s) 26
AvaI CYCGRG 2 cut(s) 2904, 3065
AvaII GGWCC 8 cut(s) 343, 1767, 2739, 4024, 4118, 4184, 4268, 4292
AvrII CCTAGG 1 cut(s) 2288
BaeGI GKGCMC 2 cut(s) 1576, 3762
BamHI GGATCC 1 cut(s) 2311
BanI GGYRCC 3 cut(s) 4, 2216, 3125
BanII GRGCYC 2 cut(s) 2288, 4080
BauI CACGAG 2 cut(s) 2766, 4179
BbsI GAAGAC 4 cut(s) 906, 1556, 1746, 3396
Bbv12I GWGCWC 3 cut(s) 1242, 1576, 3762
BbvCI CCTCAGC 2 cut(s) 1485, 1602
BceAI ACGGC 5 cut(s) 970, 1231, 2581, 3920, 3986
BciT130I CCWGG 9 cut(s) 305, 709, 1264, 1796, 2214, 2879, 2991, 4200, 4296
BclI TGATCA 2 cut(s) 1362, 1837
BcnI CCSGG 3 cut(s) 107, 1675, 3237
BcoDI GTCTC 6 cut(s) 258, 667, 1136, 2708, 3585, 3945
BcuI ACTAGT 1 cut(s) 529
BfmI CTRYAG 2 cut(s) 1962, 3713
BfoI RGCGCY 3 cut(s) 8, 3142, 3774
BfrI CTTAAG 1 cut(s) 808
BfuAI ACCTGC 2 cut(s) 1251, 2667
BglI GCCNNNNNGGC 1 cut(s) 41
BglII AGATCT 2 cut(s) 632, 3784
BlnI CCTAGG 1 cut(s) 2288
BlpI GCTNAGC 2 cut(s) 1116, 2895
BmcAI AGTACT 2 cut(s) 171, 2423
Bme18I GGWCC 8 cut(s) 343, 1767, 2739, 4024, 4118, 4184, 4268, 4292
BmeRI GACNNNNNGTC 1 cut(s) 1977
BmeT110I CYCGRG 2 cut(s) 2904, 3065
BmgBI CACGTC 1 cut(s) 2828
BmtI GCTAGC 1 cut(s) 30
BpiI GAAGAC 4 cut(s) 906, 1556, 1746, 3396
BplI GAGNNNNNCTC 4 cut(s) 347, 379, 4204, 4236
BpmI CTGGAG 4 cut(s) 657, 1817, 3489, 4111
Bpu10I CCTNAGC 3 cut(s) 39, 1485, 1602
Bpu1102I GCTNAGC 2 cut(s) 1116, 2895
BpuEI CTTGAG 2 cut(s) 3245, 3528
BpuMI CCSGG 3 cut(s) 107, 1675, 3237
Bsa29I ATCGAT 1 cut(s) 2865
BsaAI YACGTR 1 cut(s) 722
BsaBI GATNNNNATC 2 cut(s) 453, 3657
BsaHI GRCGYC 2 cut(s) 5, 4145
BsaI GGTCTC 1 cut(s) 3945
BsaJI CCNNGG 9 cut(s) 1221, 1263, 2224, 2288, 2742, 2963, 3883, 3968, 4223
BsaWI WCCGGW 2 cut(s) 2451, 2578
BsaXI ACNNNNNCTCC 4 cut(s) 2596, 2626, 3462, 3492
Bse118I RCCGGY 2 cut(s) 1940, 3135
Bse1I ACTGG 6 cut(s) 61, 923, 1510, 1776, 2618, 4094
Bse3DI GCAATG 1 cut(s) 1824
Bse8I GATNNNNATC 2 cut(s) 453, 3657
BseAI TCCGGA 1 cut(s) 2578
BseBI CCWGG 9 cut(s) 305, 709, 1264, 1796, 2214, 2879, 2991, 4200, 4296
BseCI ATCGAT 1 cut(s) 2865
BseDI CCNNGG 9 cut(s) 1221, 1263, 2224, 2288, 2742, 2963, 3883, 3968, 4223
BseJI GATNNNNATC 2 cut(s) 453, 3657
BseMI GCAATG 1 cut(s) 1824
BseMII CTCAG 9 cut(s) 53, 228, 399, 1017, 1107, 1499, 1593, 2330, 2909
BseNI ACTGG 6 cut(s) 61, 923, 1510, 1776, 2618, 4094
BseRI GAGGAG 8 cut(s) 254, 581, 2493, 2976, 3133, 3555, 3573, 3597
BseSI GKGCMC 2 cut(s) 1576, 3762
BseYI CCCAGC 1 cut(s) 770
BsgI GTGCAG 5 cut(s) 318, 510, 2298, 3503, 4086
BshFI GGCC 8 cut(s) 44, 115, 1483, 3727, 3874, 3907, 4173, 4309
BshNI GGYRCC 3 cut(s) 4, 2216, 3125
BshVI ATCGAT 1 cut(s) 2865
BsiHKAI GWGCWC 3 cut(s) 1242, 1576, 3762
BsiHKCI CYCGRG 2 cut(s) 2904, 3065
BsiSI CCGG 9 cut(s) 13, 107, 134, 1674, 1941, 2452, 2579, 3136, 3236
BslFI GGGAC 5 cut(s) 1421, 1740, 3047, 3965, 4037
BsmAI GTCTC 6 cut(s) 258, 667, 1136, 2708, 3585, 3945
BsmBI CGTCTC 3 cut(s) 667, 1136, 3585
BsmFI GGGAC 5 cut(s) 1421, 1740, 3047, 3965, 4037
BsmI GAATGC 3 cut(s) 772, 1013, 2899
BsnI GGCC 8 cut(s) 44, 115, 1483, 3727, 3874, 3907, 4173, 4309
Bso31I GGTCTC 1 cut(s) 3945
BsoBI CYCGRG 2 cut(s) 2904, 3065
Bsp1286I GDGCHC 5 cut(s) 1242, 1576, 2288, 3762, 4080
Bsp13I TCCGGA 1 cut(s) 2578
Bsp1720I GCTNAGC 2 cut(s) 1116, 2895
Bsp19I CCATGG 2 cut(s) 2224, 3883
BspANI GGCC 8 cut(s) 44, 115, 1483, 3727, 3874, 3907, 4173, 4309
BspCNI CTCAG 9 cut(s) 52, 229, 398, 1018, 1108, 1498, 1594, 2329, 2908
BspDI ATCGAT 1 cut(s) 2865
BspEI TCCGGA 1 cut(s) 2578
BspHI TCATGA 1 cut(s) 4048
BspMAI CTGCAG 2 cut(s) 1966, 3717
BspMI ACCTGC 2 cut(s) 1251, 2667
BspOI GCTAGC 1 cut(s) 30
BspPI GGATC 7 cut(s) 426, 2198, 2306, 2319, 2387, 2870, 3164
BspQI GCTCTTC 4 cut(s) 625, 1315, 2189, 3121
BspT107I GGYRCC 3 cut(s) 4, 2216, 3125
BspTI CTTAAG 1 cut(s) 808
BspTNI GGTCTC 1 cut(s) 3945
BsrBI CCGCTC 2 cut(s) 38, 53
BsrDI GCAATG 1 cut(s) 1824
BsrFI RCCGGY 2 cut(s) 1940, 3135
BsrI ACTGG 6 cut(s) 61, 923, 1510, 1776, 2618, 4094
BssAI RCCGGY 2 cut(s) 1940, 3135
BssECI CCNNGG 9 cut(s) 1221, 1263, 2224, 2288, 2742, 2963, 3883, 3968, 4223
BssNI GRCGYC 2 cut(s) 5, 4145
BssSI CACGAG 2 cut(s) 2766, 4179
BssT1I CCWWGG 5 cut(s) 1221, 2224, 2288, 2742, 3883
Bst2BI CACGAG 2 cut(s) 2766, 4179
Bst2UI CCWGG 9 cut(s) 305, 709, 1264, 1796, 2214, 2879, 2991, 4200, 4296
Bst4CI ACNGT 9 cut(s) 124, 252, 1147, 1907, 2273, 2858, 2967, 3924, 4227
Bst6I CTCTTC 7 cut(s) 261, 400, 625, 1315, 2005, 2189, 3121
BstACI GRCGYC 2 cut(s) 5, 4145
BstAFI CTTAAG 1 cut(s) 808
BstAPI GCANNNNNTGC 1 cut(s) 2696
BstBAI YACGTR 1 cut(s) 722
BstDSI CCRYGG 5 cut(s) 2224, 2963, 3883, 3968, 4223
BstEII GGTNACC 1 cut(s) 3893
BstENI CCTNNNNNAGG 3 cut(s) 548, 2211, 2734
BstH2I RGCGCY 3 cut(s) 8, 3142, 3774
BstHHI GCGC 3 cut(s) 7, 3141, 3773
BstMAI GTCTC 6 cut(s) 258, 667, 1136, 2708, 3585, 3945
BstNI CCWGG 9 cut(s) 305, 709, 1264, 1796, 2214, 2879, 2991, 4200, 4296
BstNSI RCATGY 5 cut(s) 618, 1186, 2350, 2441, 3259
BstPI GGTNACC 1 cut(s) 3893
BstSFI CTRYAG 2 cut(s) 1962, 3713
BstSLI GKGCMC 2 cut(s) 1576, 3762
BstSNI TACGTA 1 cut(s) 722
BstV2I GAAGAC 4 cut(s) 906, 1556, 1746, 3396
BstX2I RGATCY 5 cut(s) 632, 2203, 2311, 2392, 3784
BstXI CCANNNNNNTGG 2 cut(s) 1517, 4302
BstYI RGATCY 5 cut(s) 632, 2203, 2311, 2392, 3784
Bsu15I ATCGAT 1 cut(s) 2865
BsuRI GGCC 8 cut(s) 44, 115, 1483, 3727, 3874, 3907, 4173, 4309
BsuTUI ATCGAT 1 cut(s) 2865
BtgI CCRYGG 5 cut(s) 2224, 2963, 3883, 3968, 4223
BtrI CACGTC 1 cut(s) 2828
BtsI GCAGTG 5 cut(s) 964, 2652, 2961, 3026, 3762
BtsIMutI CAGTG 8 cut(s) 68, 964, 2269, 2652, 2961, 3026, 3666, 3762
BveI ACCTGC 2 cut(s) 1251, 2667
CaiI CAGNNNCTG 4 cut(s) 524, 878, 1898, 2981
CciI TCATGA 1 cut(s) 4048
CfoI GCGC 3 cut(s) 7, 3141, 3773
Cfr10I RCCGGY 2 cut(s) 1940, 3135
ClaI ATCGAT 1 cut(s) 2865
CseI GACGC 5 cut(s) 257, 649, 1004, 1550, 4134
CsiI ACCWGGT 1 cut(s) 4198
Csp6I GTAC 6 cut(s) 170, 1101, 2022, 2115, 2210, 2422
CspCI CAANNNNNGTGG 4 cut(s) 1806, 1841, 2952, 2987
CviQI GTAC 6 cut(s) 170, 1101, 2022, 2115, 2210, 2422
DinI GGCGCC 1 cut(s) 6
DraIII CACNNNGTG 2 cut(s) 2249, 3974
DriI GACNNNNNGTC 1 cut(s) 1977
EaeI YGGCCR 1 cut(s) 3905
Eam1104I CTCTTC 7 cut(s) 261, 400, 625, 1315, 2005, 2189, 3121
Eam1105I GACNNNNNGTC 1 cut(s) 1977
EarI CTCTTC 7 cut(s) 261, 400, 625, 1315, 2005, 2189, 3121
EciI GGCGGA 2 cut(s) 3576, 3696
Eco105I TACGTA 1 cut(s) 722
Eco130I CCWWGG 5 cut(s) 1221, 2224, 2288, 2742, 3883
Eco147I AGGCCT 1 cut(s) 1483
Eco24I GRGCYC 2 cut(s) 2288, 4080
Eco31I GGTCTC 1 cut(s) 3945
Eco47I GGWCC 8 cut(s) 343, 1767, 2739, 4024, 4118, 4184, 4268, 4292
Eco57I CTGAAG 6 cut(s) 456, 501, 933, 1352, 2499, 2540
Eco88I CYCGRG 2 cut(s) 2904, 3065
Eco91I GGTNACC 1 cut(s) 3893
EcoNI CCTNNNNNAGG 3 cut(s) 548, 2211, 2734
EcoO109I RGGNCCY 1 cut(s) 1767
EcoO65I GGTNACC 1 cut(s) 3893
EcoRII CCWGG 9 cut(s) 303, 707, 1262, 1794, 2212, 2877, 2989, 4198, 4294
EcoT14I CCWWGG 5 cut(s) 1221, 2224, 2288, 2742, 3883
EcoT22I ATGCAT 1 cut(s) 1013
EcoT38I GRGCYC 2 cut(s) 2288, 4080
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 5 cut(s) 1221, 2224, 2288, 2742, 3883
Esp3I CGTCTC 3 cut(s) 667, 1136, 3585
FalI AAGNNNNNCTT 4 cut(s) 429, 461, 542, 574
FaqI GGGAC 5 cut(s) 1421, 1740, 3047, 3965, 4037
FauI CCCGC 2 cut(s) 3260, 3990
FauNDI CATATG 1 cut(s) 3087
FbaI TGATCA 2 cut(s) 1362, 1837
FriOI GRGCYC 2 cut(s) 2288, 4080
GlaI GCGC 3 cut(s) 6, 3140, 3772
GsaI CCCAGC 1 cut(s) 774
GsuI CTGGAG 4 cut(s) 657, 1817, 3489, 4111
HaeII RGCGCY 3 cut(s) 8, 3142, 3774
HaeIII GGCC 8 cut(s) 44, 115, 1483, 3727, 3874, 3907, 4173, 4309
HapII CCGG 9 cut(s) 13, 107, 134, 1674, 1941, 2452, 2579, 3136, 3236
HgaI GACGC 5 cut(s) 257, 649, 1004, 1550, 4134
HhaI GCGC 3 cut(s) 7, 3141, 3773
Hin1I GRCGYC 2 cut(s) 5, 4145
Hin6I GCGC 3 cut(s) 5, 3139, 3771
HinP1I GCGC 3 cut(s) 5, 3139, 3771
HincII GTYRAC 2 cut(s) 391, 3442
HindII GTYRAC 2 cut(s) 391, 3442
HindIII AAGCTT 1 cut(s) 805
HpaII CCGG 9 cut(s) 13, 107, 134, 1674, 1941, 2452, 2579, 3136, 3236
Hpy166II GTNNAC 6 cut(s) 391, 1574, 2264, 3442, 3760, 4184
Hpy8I GTNNAC 6 cut(s) 391, 1574, 2264, 3442, 3760, 4184
Hpy99I CGWCG 2 cut(s) 956, 3084
HpyCH4III ACNGT 9 cut(s) 124, 252, 1147, 1907, 2273, 2858, 2967, 3924, 4227
HpyCH4IV ACGT 6 cut(s) 721, 2189, 2337, 2827, 3393, 3647
HpySE526I ACGT 6 cut(s) 721, 2189, 2337, 2827, 3393, 3647
Hsp92I GRCGYC 2 cut(s) 5, 4145
HspAI GCGC 3 cut(s) 5, 3139, 3771
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 2578
KroI GCCGGC 1 cut(s) 3135
KroNI GCCGGC 1 cut(s) 3137
Ksp22I TGATCA 2 cut(s) 1362, 1837
LguI GCTCTTC 4 cut(s) 625, 1315, 2189, 3121
MabI ACCWGGT 1 cut(s) 4198
MaeII ACGT 6 cut(s) 721, 2189, 2337, 2827, 3393, 3647
MaeIII GTNAC 8 cut(s) 146, 497, 1619, 1687, 2319, 3310, 3893, 4279
MbiI CCGCTC 2 cut(s) 38, 53
MfeI CAATTG 1 cut(s) 2889
MflI RGATCY 5 cut(s) 632, 2203, 2311, 2392, 3784
MhlI GDGCHC 5 cut(s) 1242, 1576, 2288, 3762, 4080
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 7 cut(s) 386, 1403, 1736, 2852, 3077, 3480, 3665
MmeI TCCRAC 3 cut(s) 2331, 2793, 3027
Mph1103I ATGCAT 1 cut(s) 1013
MroI TCCGGA 1 cut(s) 2578
MroNI GCCGGC 1 cut(s) 3135
MroXI GAANNNNTTC 5 cut(s) 441, 918, 1216, 3217, 3283
MseI TTAA 8 cut(s) 809, 864, 1035, 1158, 1881, 2145, 2468, 3735
MslI CAYNNNNRTG 2 cut(s) 2274, 3765
MspA1I CMGCKG 4 cut(s) 1115, 1601, 2978, 3602
MspCI CTTAAG 1 cut(s) 808
MspI CCGG 9 cut(s) 13, 107, 134, 1674, 1941, 2452, 2579, 3136, 3236
MunI CAATTG 1 cut(s) 2889
Mva1269I GAATGC 3 cut(s) 772, 1013, 2899
MvaI CCWGG 9 cut(s) 305, 709, 1264, 1796, 2214, 2879, 2991, 4200, 4296
NaeI GCCGGC 1 cut(s) 3137
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 3 cut(s) 107, 1675, 3237
NcoI CCATGG 2 cut(s) 2224, 3883
NdeI CATATG 1 cut(s) 3087
NgoMIV GCCGGC 1 cut(s) 3135
NheI GCTAGC 1 cut(s) 26
NmuCI GTSAC 3 cut(s) 2319, 3310, 4279
NsiI ATGCAT 1 cut(s) 1013
NspI RCATGY 5 cut(s) 618, 1186, 2350, 2441, 3259
OliI CACNNNNGTG 1 cut(s) 2274
PaeI GCATGC 2 cut(s) 1186, 3259
PaeR7I CTCGAG 1 cut(s) 2904
PagI TCATGA 1 cut(s) 4048
PaqCI CACCTGC 1 cut(s) 1251
PceI AGGCCT 1 cut(s) 1483
PciI ACATGT 2 cut(s) 2346, 2437
PciSI GCTCTTC 4 cut(s) 625, 1315, 2189, 3121
PcsI WCGNNNNNNNCGW 2 cut(s) 2764, 3088
PctI GAATGC 3 cut(s) 772, 1013, 2899
PdiI GCCGGC 1 cut(s) 3137
PdmI GAANNNNTTC 5 cut(s) 441, 918, 1216, 3217, 3283
PflMI CCANNNNNTGG 1 cut(s) 1898
PfoI TCCNGGA 7 cut(s) 303, 707, 1673, 1794, 2989, 3235, 4294
PleI GAGTC 7 cut(s) 386, 1403, 1736, 2851, 3076, 3479, 3665
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 7 cut(s) 386, 1403, 1736, 2851, 3076, 3479, 3665
Ppu21I YACGTR 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 1767
PscI ACATGT 2 cut(s) 2346, 2437
Psp5II RGGWCCY 1 cut(s) 1767
Psp6I CCWGG 9 cut(s) 303, 707, 1262, 1794, 2212, 2877, 2989, 4198, 4294
PspEI GGTNACC 1 cut(s) 3893
PspFI CCCAGC 1 cut(s) 770
PspGI CCWGG 9 cut(s) 303, 707, 1262, 1794, 2212, 2877, 2989, 4198, 4294
PspPPI RGGWCCY 1 cut(s) 1767
PsrI GAACNNNNNNTAC 2 cut(s) 2023, 2055
PstI CTGCAG 2 cut(s) 1966, 3717
PstNI CAGNNNCTG 4 cut(s) 524, 878, 1898, 2981
PsuI RGATCY 5 cut(s) 632, 2203, 2311, 2392, 3784
PvuII CAGCTG 3 cut(s) 1115, 1601, 2978
RsaI GTAC 6 cut(s) 171, 1102, 2023, 2116, 2211, 2423
RsaNI GTAC 6 cut(s) 170, 1101, 2022, 2115, 2210, 2422
RseI CAYNNNNRTG 2 cut(s) 2274, 3765
SapI GCTCTTC 4 cut(s) 625, 1315, 2189, 3121
SaqAI TTAA 8 cut(s) 809, 864, 1035, 1158, 1881, 2145, 2468, 3735
ScaI AGTACT 2 cut(s) 171, 2423
SchI GAGTC 7 cut(s) 386, 1403, 1736, 2852, 3077, 3480, 3665
SduI GDGCHC 5 cut(s) 1242, 1576, 2288, 3762, 4080
SexAI ACCWGGT 1 cut(s) 4198
SfcI CTRYAG 2 cut(s) 1962, 3713
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 2904
SinI GGWCC 8 cut(s) 343, 1767, 2739, 4024, 4118, 4184, 4268, 4292
SlaI CTCGAG 1 cut(s) 2904
SmiMI CAYNNNNRTG 2 cut(s) 2274, 3765
SmlI CTYRAG 4 cut(s) 808, 2904, 3260, 3543
SmoI CTYRAG 4 cut(s) 808, 2904, 3260, 3543
SnaBI TACGTA 1 cut(s) 722
SpeI ACTAGT 1 cut(s) 529
SphI GCATGC 2 cut(s) 1186, 3259
SseBI AGGCCT 1 cut(s) 1483
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 2 cut(s) 2551, 3856
StuI AGGCCT 1 cut(s) 1483
StyI CCWWGG 5 cut(s) 1221, 2224, 2288, 2742, 3883
TaaI ACNGT 9 cut(s) 124, 252, 1147, 1907, 2273, 2858, 2967, 3924, 4227
TaiI ACGT 6 cut(s) 724, 2192, 2340, 2830, 3396, 3650
TaqI TCGA 6 cut(s) 1499, 2758, 2846, 2865, 2905, 3201
TaqII GACCGA 1 cut(s) 4041
TatI WGTACW 2 cut(s) 169, 2421
TauI GCSGC 8 cut(s) 38, 47, 50, 53, 60, 3605, 3988, 4309
Tru1I TTAA 8 cut(s) 809, 864, 1035, 1158, 1881, 2145, 2468, 3735
Tru9I TTAA 8 cut(s) 809, 864, 1035, 1158, 1881, 2145, 2468, 3735
TscAI CASTG 8 cut(s) 68, 964, 2276, 2652, 2961, 3026, 3673, 3762
TseFI GTSAC 3 cut(s) 2319, 3310, 4279
Tsp45I GTSAC 3 cut(s) 2319, 3310, 4279
TspGWI ACGGA 9 cut(s) 728, 838, 844, 1675, 1867, 3068, 3225, 3417, 4152
TspRI CASTG 8 cut(s) 68, 964, 2276, 2652, 2961, 3026, 3673, 3762
Van91I CCANNNNNTGG 1 cut(s) 1898
Vha464I CTTAAG 1 cut(s) 808
VneI GTGCAC 2 cut(s) 1572, 3758
VpaK11BI GGWCC 8 cut(s) 343, 1767, 2739, 4024, 4118, 4184, 4268, 4292
XagI CCTNNNNNAGG 3 cut(s) 548, 2211, 2734
XapI RAATTY 7 cut(s) 1213, 1920, 1987, 2081, 2817, 3297, 3841
XbaI TCTAGA 3 cut(s) 1432, 3146, 3779
XceI RCATGY 5 cut(s) 618, 1186, 2350, 2441, 3259
XcmI CCANNNNNNNNNTGG 1 cut(s) 1517
XhoI CTCGAG 1 cut(s) 2904
XmaJI CCTAGG 1 cut(s) 2288
XmnI GAANNNNTTC 5 cut(s) 441, 918, 1216, 3217, 3283
ZrmI AGTACT 2 cut(s) 171, 2423
Zsp2I ATGCAT 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.