Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002982

Physical Location & Seq

Genomic Coordinates Forward (+)

19773098 .. 19777168

Gene Structure 4071 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g24221.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2484 bp

ATGGTCAATAGTGGTGGTACCGGCAGTAACCAAGGGATGTCATCCAAGAAGAGACGAGAGAAGACTAAAGCAGTAGCTCTTTGTTGTACAAAGAAAAAGCCAGCTGAAGCTTCTCCAGGGAGTGTAAATAAGCCTCTGAGTGAAATACATCCAGAGGCATGTAGAAAACGCAGTGAACCTGAGCCGGCAGAGGCTATGGCGGTTCAGCCTAAGGAAGAATTGGAAATGGGAGCCGATGAAGGTAGAGATTGGGATGATCCTATTGCATGCCAGCTTGAAGAACTATTGTTGTCTTATTTGCAAGCAATTTTCCAGAGTGCAATCAAGCGGATTACTGAATGTGGGTACAGTGAGGAGGTTGCTGTGAAGGCTGTTTCTAGAGGCGGCCTTTACATAGGCGGTAAGGACTCTGTATTGAACATTGTAAATGATACTCTGGAATTTCTCGAGGGAGGGAAGGATACTGATACTTCAAGAGAAAATGAGTTTGATAATTTACAGCACCTGGTGGAGTACACGATGCTAGAAATGATTAGTGTGCTTAGGGAGGTCAGGCCATCCTTATCAGTTGCAGAAGCAATGTGGTGGTTATTGATATGTGACCTAAACATTTTGCTAGCGTGTACAATGGAAGGAGATCTATCAAGTGTTCTTGCTTTTGAAGAATCCTCAACAGAGAGCTCCCCTGATTCTTCCTCTTCCCAATTACAATCAGAGGCAAATAATGAAACAAACCATCCTAATCCTAGCAAAGAAAATTCCTCCAAGCCATCACTTCCCAACTCTCAGAACCACCCTTCTCTCTCTGAAACAGTTAAATTTGGAAATTTATCAAACTCTGCTAACCCTGAAACGCCAGAAGGCAAGGAGGAGAGTTTGTCATCAATGCTTGATAGTTTGGAGAAATGCTTGGGATCCACAAGGGATTATGTCCTATCTTCATCTCAGACATCTACCTCTGAAGAAAAATCAGGAGCTAGTAGAAAGGGGCGGACCAAGGCAGAATTAGCAGCACTTCGGAAGAGATCCTTGCAGATGGAGAGAAACTACAGGGCTTATAGGTCAAAAGGAGGCTTCAAATCAGGGAAGCTTGCTGCATTTGGTAGTTTTCTTATGGAAAGGAGGGCAAAGCCTCCATCTGATCTTCCTGAAGCTGGAACAAAAGATTCCTCCTCAAAGGTTACTGTACCAGGAGGAACCAGTAATCCATCTGCTTCACCTGCAAGAGGTTGCTCCTCAACAGTACCTTCAAAAGGTACCACATCTTCATTGTCTTCTGTGGATACCAAGCAGAAACAGAAGTCTGATTCAGAAGAAAAATCTGAAACTAAGACTCCAGTCTGCACCTCTGTTCCCCCCAAGACTGTTGATTACTGTGCTGGCATCCCATATGATGAGACTTTAGGGAAGTATGTCCCACAAGATAAGAAGGATGAGCTGATTATGAAGCTAGTTCCCCAGTTGCAGAAACTGCAGAATGAGCTACAGAGTTGGACAGAGTGGGCCAATGAGAAGGTTATGCAGGTTTCTCGTAGACTTGGCAAAGAACGACCTGAACTTAAAGCACTGAGGCAAGAGAAGGAAGAAGCGGGAAAATTTGAAAAAGAGAAACAAATGCTGGATGAGAATACCATGAAGAGGCTGTCTGAGATGGAGCATGCCTTGAATAATGCATCCAGCCAGGTGGACGGTGCTAACGCAACTATCTCTAGGCTTCAGGAGAACAATTCCACTCTCAAGAAGCAGTTGAGTTCTGCCAAAATACTGTCTATAGAATCAGCTGCTAACCTTCGAGAAGCATTAGAGAGGGAGAAGAAAGCACTGAAAAAGGTCCAGGCATGGGAGGGGCAGAAGAGTTCATTGAAGGAGGAGCTTGAAACAAAAAAGAACAAGGTGGCTGTGTTGCAACAGGATTTAGGCAAGGCAAAAAATACTCTTCAACAAATTGAGGCCAGATGGAAGCGGGTGATAAGGGAAAAGGAAATAATACTCGCACAGGCTGCCTCTATGAGAAATGAAAGAGAACAACTTGAAGCTTTGACATTAGTGGAGGAGGCCAAGATGAAACTCAAGGCAGAAGATGATATGCAGAAGTATGTGGAAGAGATCAAATTACTTGAGAGGAAGTTATCTGAACTTCAAATAGAATCTGACTCCTCAAGAATAGCAGCGCTAAGAAGGGATGCTACTGGAAGCTTTGGGGCTGGCCTGGCAGACAGGAAAAATGTGGTGGGTACTAAGGCAAACCAAAGTTCTACCGTCTCTAAGAAGTTAACAAATTCCCGGGGGACTGAAAGTTTGAGACAGGACCGCGAGTGTGTGATGTGCCTATCTGAGGAGATGTCAGTGGTGTTCCTCCCGTGCGCACATCAGGTCTTGTGTGCAACATGCAATGAGCTGCATCTGAAACAAGGAATGAAGGACTGCCCTTCATGTAGAGCTTTAATTCAGCGAAGGATTAAAGTGCAATATGCTCGTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009987 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

828

Amino Acids

91.49

Weight (kDa)

7.94

Isoelectric Point (pI)

60.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIR2-like_helical	PF20235	100 - 211	3.4e-21	PIR2-like, helical domain
zf-C3HC4_3	PF13920	770 - 818	1.9e-11	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002982)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03365 AT1G03365 AT4G03000 AT4G03000 AT4G03000 AT4G03000 AT4G03000 AT4G03000
fragaria_vesca	FvH4_2g24221 FvH4_2g24221 FvH4_2g24221
malus_domestica	MD03G1272000.v1.1 MD11G1292600.v1.1
prunus_persica	Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1
pyrus_communis	pycom03g21660 pycom11g25900
rosa_chinensis	RchiOBHm_Chr6g0292011
rosa_laevigata	RLG00000012070
rosa_multiflora	Rmu_co8265653.1_g000001 Rmu_co8382791.1_g000001 Rmu_sc0008666.1_g000003
rosa_roxburghii	Rroxscaffold_7G00175490 Rroxscaffold_7G00178030
rosa_rugosa	Rorug06G0222500 Rorug06G0222600 Rorug06G0222700 Rorug06G0222800 Rorug06G0222900
rosa_samantha	Rh6AG336400 Rh6BG343700 Rh6CG350000 Rh6DG336700
rosa_wichuraiana	Rw6G029280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1228
Acc16I	TGCGCA	1 cut(s)	2365
Acc36I	ACCTGC	2 cut(s)	1228, 1513
Acc65I	GGTACC	2 cut(s)	17, 1256
AccB1I	GGYRCC	2 cut(s)	17, 1256
AccI	GTMKAC	1 cut(s)	1534
AccII	CGCG	1 cut(s)	2313
AciI	CCGC	8 cut(s)	200, 328, 384, 399, 991, 1589, 1963, 2311
AclWI	GGATC	4 cut(s)	251, 909, 922, 1020
AcsI	RAATTY	6 cut(s)	440, 757, 818, 826, 1595, 2278
AcuI	CTGAAG	4 cut(s)	126, 981, 1170, 1700
AdeI	CACNNNGTG	1 cut(s)	508
AfaI	GTAC	9 cut(s)	19, 88, 347, 515, 625, 1188, 1245, 1258, 2236
AfeI	AGCGCT	1 cut(s)	2172
AfiI	CCNNNNNNNGG	6 cut(s)	1154, 1226, 1253, 1638, 1840, 2335
AjnI	CCWGG	6 cut(s)	115, 504, 1189, 1680, 1833, 2208
AloI	GAACNNNNNNTCC	2 cut(s)	1189, 1221
Alw21I	GWGCWC	1 cut(s)	683
Alw26I	GTCTC	4 cut(s)	46, 1391, 2266, 2296
AlwI	GGATC	4 cut(s)	251, 909, 922, 1020
AlwNI	CAGNNNCTG	2 cut(s)	505, 1471
Ama87I	CYCGRG	2 cut(s)	446, 2283
Aor51HI	AGCGCT	1 cut(s)	2172
AoxI	GGCC	6 cut(s)	385, 554, 1503, 1950, 2055, 2206
ApeKI	GCWGC	6 cut(s)	1010, 1094, 1781, 2000, 2168, 2398
ApoI	RAATTY	6 cut(s)	440, 757, 818, 826, 1595, 2278
ArsI	GACNNNNNNTTYG	2 cut(s)	2281, 2313
Asp718I	GGTACC	2 cut(s)	17, 1256
AspLEI	GCGC	2 cut(s)	2173, 2366
AspS9I	GGNCC	4 cut(s)	993, 1503, 1831, 2308
AsuC2I	CCSGG	2 cut(s)	2284, 2285
AsuHPI	GGTGA	2 cut(s)	1209, 1978
AsuNHI	GCTAGC	1 cut(s)	616
AvaI	CYCGRG	2 cut(s)	446, 2283
AvaII	GGWCC	3 cut(s)	993, 1831, 2308
AxyI	CCTNAGG	1 cut(s)	210
BamHI	GGATCC	1 cut(s)	914
BanI	GGYRCC	2 cut(s)	17, 1256
BanII	GRGCYC	1 cut(s)	683
BarI	GAAGNNNNNNTAC	4 cut(s)	1249, 1281, 2170, 2202
BbsI	GAAGAC	2 cut(s)	68, 1266
Bbv12I	GWGCWC	1 cut(s)	683
BbvI	GCAGC	6 cut(s)	1022, 1081, 1768, 1987, 2180, 2385
BccI	CCATC	8 cut(s)	565, 744, 778, 1030, 1144, 1216, 1645, 1950
BcgI	CGANNNNNNTGC	3 cut(s)	1511, 1545, 2456
BciT130I	CCWGG	6 cut(s)	117, 506, 1191, 1682, 1835, 2210
BciVI	GTATCC	2 cut(s)	454, 1276
BcnI	CCSGG	2 cut(s)	2284, 2285
BcoDI	GTCTC	4 cut(s)	46, 1391, 2266, 2296
BfaI	CTAG	7 cut(s)	378, 524, 617, 747, 978, 1451, 1710
BfmI	CTRYAG	4 cut(s)	1048, 1472, 1484, 1770
BfoI	RGCGCY	1 cut(s)	2174
BfuAI	ACCTGC	2 cut(s)	1228, 1513
BfuI	GTATCC	2 cut(s)	454, 1276
BglII	AGATCT	1 cut(s)	637
BisI	GCNGC	7 cut(s)	385, 1011, 1095, 1782, 2001, 2169, 2399
BlsI	GCNGC	7 cut(s)	386, 1012, 1096, 1783, 2002, 2170, 2400
Bme1390I	CCNGG	8 cut(s)	117, 506, 1191, 1682, 1835, 2210, 2284, 2285
Bme18I	GGWCC	3 cut(s)	993, 1831, 2308
BmeT110I	CYCGRG	2 cut(s)	446, 2283
BmgT120I	GGNCC	4 cut(s)	993, 1503, 1831, 2308
BmiI	GGNNCC	5 cut(s)	19, 232, 916, 1198, 1258
BmrFI	CCNGG	8 cut(s)	117, 506, 1191, 1682, 1835, 2210, 2284, 2285
BmrI	ACTGGG	1 cut(s)	1453
BmsI	GCATC	5 cut(s)	510, 1392, 1682, 2173, 2410
BmtI	GCTAGC	1 cut(s)	620
BmuI	ACTGGG	1 cut(s)	1453
BoxI	GACNNNNGTC	1 cut(s)	1337
BpiI	GAAGAC	2 cut(s)	68, 1266
BpmI	CTGGAG	2 cut(s)	99, 1320
Bpu10I	CCTNAGC	2 cut(s)	180, 542
BpuEI	CTTGAG	4 cut(s)	1721, 2054, 2138, 2143
BpuMI	CCSGG	2 cut(s)	2284, 2285
BsaJI	CCNNGG	5 cut(s)	31, 116, 996, 2283, 2284
BsaXI	ACNNNNNCTCC	4 cut(s)	863, 893, 1318, 1348
Bsc4I	CCNNNNNNNGG	6 cut(s)	1154, 1226, 1253, 1638, 1840, 2335
Bse118I	RCCGGY	2 cut(s)	20, 184
Bse1I	ACTGG	4 cut(s)	1200, 1337, 1459, 2194
Bse21I	CCTNAGG	1 cut(s)	210
Bse3DI	GCAATG	2 cut(s)	585, 2398
BseBI	CCWGG	6 cut(s)	117, 506, 1191, 1682, 1835, 2210
BseDI	CCNNGG	5 cut(s)	31, 116, 996, 2283, 2284
BseLI	CCNNNNNNNGG	6 cut(s)	1154, 1226, 1253, 1638, 1840, 2335
BseMI	GCAATG	2 cut(s)	585, 2398
BseMII	CTCAG	7 cut(s)	128, 171, 800, 959, 1559, 1638, 2325
BseNI	ACTGG	4 cut(s)	1200, 1337, 1459, 2194
BseRI	GAGGAG	8 cut(s)	368, 884, 1162, 1225, 1883, 2066, 2146, 2351
BseXI	GCAGC	6 cut(s)	1022, 1081, 1768, 1987, 2180, 2385
BsgI	GTGCAG	1 cut(s)	1327
Bsh1236I	CGCG	1 cut(s)	2313
BshFI	GGCC	6 cut(s)	387, 556, 1505, 1952, 2057, 2208
BshNI	GGYRCC	2 cut(s)	17, 1256
BsiHKAI	GWGCWC	1 cut(s)	683
BsiHKCI	CYCGRG	2 cut(s)	446, 2283
BsiSI	CCGG	3 cut(s)	21, 185, 2284
BslFI	GGGAC	2 cut(s)	1400, 2302
BslI	CCNNNNNNNGG	6 cut(s)	1154, 1226, 1253, 1638, 1840, 2335
BsmAI	GTCTC	4 cut(s)	46, 1391, 2266, 2296
BsmBI	CGTCTC	2 cut(s)	46, 2266
BsmFI	GGGAC	2 cut(s)	1400, 2302
BsnI	GGCC	6 cut(s)	387, 556, 1505, 1952, 2057, 2208
BsoBI	CYCGRG	2 cut(s)	446, 2283
Bsp1286I	GDGCHC	1 cut(s)	683
Bsp1407I	TGTACA	2 cut(s)	86, 623
Bsp143I	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
BspACI	CCGC	8 cut(s)	200, 328, 384, 399, 991, 1589, 1963, 2311
BspANI	GGCC	6 cut(s)	387, 556, 1505, 1952, 2057, 2208
BspCNI	CTCAG	7 cut(s)	129, 172, 799, 958, 1560, 1639, 2326
BspFNI	CGCG	1 cut(s)	2313
BspLI	GGNNCC	5 cut(s)	19, 232, 916, 1198, 1258
BspMAI	CTGCAG	1 cut(s)	1476
BspMI	ACCTGC	2 cut(s)	1228, 1513
BspOI	GCTAGC	1 cut(s)	620
BspPI	GGATC	4 cut(s)	251, 909, 922, 1020
BspT107I	GGYRCC	2 cut(s)	17, 1256
BsrDI	GCAATG	2 cut(s)	585, 2398
BsrFI	RCCGGY	2 cut(s)	20, 184
BsrGI	TGTACA	2 cut(s)	86, 623
BsrI	ACTGG	4 cut(s)	1200, 1337, 1459, 2194
BssAI	RCCGGY	2 cut(s)	20, 184
BssECI	CCNNGG	5 cut(s)	31, 116, 996, 2283, 2284
BssMI	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
BssT1I	CCWWGG	2 cut(s)	31, 996
Bst2UI	CCWGG	6 cut(s)	117, 506, 1191, 1682, 1835, 2210
Bst4CI	ACNGT	9 cut(s)	350, 814, 1186, 1243, 1366, 1376, 1691, 1767, 2260
Bst6I	CTCTTC	7 cut(s)	44, 703, 1016, 1631, 1847, 1941, 2097
BstAPI	GCANNNNNTGC	2 cut(s)	1471, 2000
BstAUI	TGTACA	2 cut(s)	86, 623
BstENI	CCTNNNNNAGG	3 cut(s)	1224, 1251, 2333
BstFNI	CGCG	1 cut(s)	2313
BstH2I	RGCGCY	1 cut(s)	2174
BstHHI	GCGC	2 cut(s)	2173, 2366
BstKTI	GATC	6 cut(s)	259, 640, 917, 1028, 1144, 2109
BstMAI	GTCTC	4 cut(s)	46, 1391, 2266, 2296
BstMBI	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
BstMWI	GCNNNNNNNGC	7 cut(s)	368, 1007, 1220, 1471, 1480, 2000, 2210
BstNI	CCWGG	6 cut(s)	117, 506, 1191, 1682, 1835, 2210
BstNSI	RCATGY	4 cut(s)	162, 270, 1661, 2391
BstPAI	GACNNNNGTC	1 cut(s)	1337
BstSCI	CCNGG	8 cut(s)	115, 504, 1189, 1680, 1833, 2208, 2282, 2283
BstSFI	CTRYAG	4 cut(s)	1048, 1472, 1484, 1770
BstUI	CGCG	1 cut(s)	2313
BstV1I	GCAGC	6 cut(s)	1022, 1081, 1768, 1987, 2180, 2385
BstV2I	GAAGAC	2 cut(s)	68, 1266
BstX2I	RGATCY	3 cut(s)	637, 914, 1025
BstXI	CCANNNNNNTGG	1 cut(s)	1684
BstYI	RGATCY	3 cut(s)	637, 914, 1025
Bsu36I	CCTNAGG	1 cut(s)	210
BsuI	GTATCC	2 cut(s)	454, 1276
BsuRI	GGCC	6 cut(s)	387, 556, 1505, 1952, 2057, 2208
BtsI	GCAGTG	1 cut(s)	178
BtsIMutI	CAGTG	5 cut(s)	178, 355, 1565, 1820, 2352
BveI	ACCTGC	2 cut(s)	1228, 1513
CaiI	CAGNNNCTG	2 cut(s)	505, 1471
CfoI	GCGC	2 cut(s)	2173, 2366
Cfr10I	RCCGGY	2 cut(s)	20, 184
Cfr13I	GGNCC	4 cut(s)	993, 1503, 1831, 2308
Cfr9I	CCCGGG	1 cut(s)	2283
CsiI	ACCWGGT	1 cut(s)	504
Csp6I	GTAC	9 cut(s)	18, 87, 346, 514, 624, 1187, 1244, 1257, 2235
CspCI	CAANNNNNGTGG	1 cut(s)	30
CviAII	CATG	7 cut(s)	159, 267, 1633, 1658, 1839, 2388, 2433
CviQI	GTAC	9 cut(s)	18, 87, 346, 514, 624, 1187, 1244, 1257, 2235
DpnI	GATC	6 cut(s)	258, 639, 916, 1027, 1143, 2108
DpnII	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
DraIII	CACNNNGTG	1 cut(s)	508
Eam1104I	CTCTTC	7 cut(s)	44, 703, 1016, 1631, 1847, 1941, 2097
EarI	CTCTTC	7 cut(s)	44, 703, 1016, 1631, 1847, 1941, 2097
EciI	GGCGGA	1 cut(s)	1006
Ecl136II	GAGCTC	1 cut(s)	681
Eco130I	CCWWGG	2 cut(s)	31, 996
Eco24I	GRGCYC	1 cut(s)	683
Eco47I	GGWCC	3 cut(s)	993, 1831, 2308
Eco47III	AGCGCT	1 cut(s)	2172
Eco53kI	GAGCTC	1 cut(s)	681
Eco57I	CTGAAG	4 cut(s)	126, 981, 1170, 1700
Eco81I	CCTNAGG	1 cut(s)	210
Eco88I	CYCGRG	2 cut(s)	446, 2283
EcoICRI	GAGCTC	1 cut(s)	681
EcoNI	CCTNNNNNAGG	3 cut(s)	1224, 1251, 2333
EcoRII	CCWGG	6 cut(s)	115, 504, 1189, 1680, 1833, 2208
EcoT14I	CCWWGG	2 cut(s)	31, 996
EcoT22I	ATGCAT	1 cut(s)	1675
EcoT38I	GRGCYC	1 cut(s)	683
ErhI	CCWWGG	2 cut(s)	31, 996
Esp3I	CGTCTC	2 cut(s)	46, 2266
FaeI	CATG	7 cut(s)	162, 270, 1636, 1661, 1842, 2391, 2436
FalI	AAGNNNNNCTT	2 cut(s)	1013, 1045
FaqI	GGGAC	2 cut(s)	1400, 2302
FatI	CATG	7 cut(s)	158, 266, 1632, 1657, 1838, 2387, 2432
FauI	CCCGC	2 cut(s)	1582, 1956
FauNDI	CATATG	1 cut(s)	1390
FblI	GTMKAC	1 cut(s)	1534
Fnu4HI	GCNGC	7 cut(s)	385, 1011, 1095, 1782, 2001, 2169, 2399
FriOI	GRGCYC	1 cut(s)	683
Fsp4HI	GCNGC	7 cut(s)	385, 1011, 1095, 1782, 2001, 2169, 2399
FspAI	RTGCGCAY	1 cut(s)	2365
FspBI	CTAG	7 cut(s)	378, 524, 617, 747, 978, 1451, 1710
FspI	TGCGCA	1 cut(s)	2365
GlaI	GCGC	2 cut(s)	2172, 2365
GluI	GCNGC	7 cut(s)	385, 1011, 1095, 1782, 2001, 2169, 2399
GsuI	CTGGAG	2 cut(s)	99, 1320
HaeII	RGCGCY	1 cut(s)	2174
HaeIII	GGCC	6 cut(s)	387, 556, 1505, 1952, 2057, 2208
HapII	CCGG	3 cut(s)	21, 185, 2284
HhaI	GCGC	2 cut(s)	2173, 2366
Hin1II	CATG	7 cut(s)	162, 270, 1636, 1661, 1842, 2391, 2436
Hin6I	GCGC	2 cut(s)	2171, 2364
HinP1I	GCGC	2 cut(s)	2171, 2364
HincII	GTYRAC	1 cut(s)	2274
HindII	GTYRAC	1 cut(s)	2274
HindIII	AAGCTT	4 cut(s)	108, 1088, 2034, 2194
HinfI	GANTC	9 cut(s)	407, 665, 689, 1166, 1307, 1333, 1775, 2147, 2153
HpaI	GTTAAC	1 cut(s)	2274
HpaII	CCGG	3 cut(s)	21, 185, 2284
HphI	GGTGA	2 cut(s)	1209, 1978
Hpy166II	GTNNAC	6 cut(s)	176, 516, 624, 1535, 1687, 2274
Hpy8I	GTNNAC	6 cut(s)	176, 516, 624, 1535, 1687, 2274
HpyCH4III	ACNGT	9 cut(s)	350, 814, 1186, 1243, 1366, 1376, 1691, 1767, 2260
HpyF10VI	GCNNNNNNNGC	7 cut(s)	368, 1007, 1220, 1471, 1480, 2000, 2210
Hsp92II	CATG	7 cut(s)	162, 270, 1636, 1661, 1842, 2391, 2436
HspAI	GCGC	2 cut(s)	2171, 2364
KpnI	GGTACC	2 cut(s)	21, 1260
KroI	GCCGGC	1 cut(s)	184
KroNI	GCCGGC	1 cut(s)	186
KspAI	GTTAAC	1 cut(s)	2274
Kzo9I	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
LmnI	GCTCC	6 cut(s)	230, 686, 974, 1238, 1654, 1870
Lsp1109I	GCAGC	6 cut(s)	1022, 1081, 1768, 1987, 2180, 2385
LweI	GCATC	5 cut(s)	510, 1392, 1682, 2173, 2410
MabI	ACCWGGT	1 cut(s)	504
MaeI	CTAG	7 cut(s)	378, 524, 617, 747, 978, 1451, 1710
MaeIII	GTNAC	3 cut(s)	26, 599, 1180
MalI	GATC	6 cut(s)	258, 639, 916, 1027, 1143, 2108
MboI	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
MflI	RGATCY	3 cut(s)	637, 914, 1025
MhlI	GDGCHC	1 cut(s)	683
MlyI	GAGTC	3 cut(s)	401, 1327, 2147
MmeI	TCCRAC	1 cut(s)	1472
Mph1103I	ATGCAT	1 cut(s)	1675
MroNI	GCCGGC	1 cut(s)	184
MseI	TTAA	5 cut(s)	816, 1560, 2273, 2444, 2460
MspA1I	CMGCKG	2 cut(s)	104, 1781
MspI	CCGG	3 cut(s)	21, 185, 2284
MspR9I	CCNGG	8 cut(s)	117, 506, 1191, 1682, 1835, 2210, 2284, 2285
MvaI	CCWGG	6 cut(s)	117, 506, 1191, 1682, 1835, 2210
MvnI	CGCG	1 cut(s)	2313
MwoI	GCNNNNNNNGC	7 cut(s)	368, 1007, 1220, 1471, 1480, 2000, 2210
NaeI	GCCGGC	1 cut(s)	186
NciI	CCSGG	2 cut(s)	2284, 2285
NdeI	CATATG	1 cut(s)	1390
NdeII	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
NgoMIV	GCCGGC	1 cut(s)	184
NheI	GCTAGC	1 cut(s)	616
NlaIII	CATG	7 cut(s)	162, 270, 1636, 1661, 1842, 2391, 2436
NlaIV	GGNNCC	5 cut(s)	19, 232, 916, 1198, 1258
NmuCI	GTSAC	1 cut(s)	599
NsbI	TGCGCA	1 cut(s)	2365
NsiI	ATGCAT	1 cut(s)	1675
NspI	RCATGY	4 cut(s)	162, 270, 1661, 2391
PaeI	GCATGC	2 cut(s)	270, 1661
PaeR7I	CTCGAG	1 cut(s)	446
PaqCI	CACCTGC	1 cut(s)	1228
PdiI	GCCGGC	1 cut(s)	186
PfeI	GAWTC	6 cut(s)	665, 689, 1166, 1307, 1775, 2147
PkrI	GCNGC	7 cut(s)	386, 1012, 1096, 1783, 2002, 2170, 2400
PleI	GAGTC	3 cut(s)	401, 1327, 2147
PpsI	GAGTC	3 cut(s)	401, 1327, 2147
PshAI	GACNNNNGTC	1 cut(s)	1337
Psp124BI	GAGCTC	1 cut(s)	683
Psp6I	CCWGG	6 cut(s)	115, 504, 1189, 1680, 1833, 2208
PspGI	CCWGG	6 cut(s)	115, 504, 1189, 1680, 1833, 2208
PspN4I	GGNNCC	5 cut(s)	19, 232, 916, 1198, 1258
PspPI	GGNCC	4 cut(s)	993, 1503, 1831, 2308
PstI	CTGCAG	1 cut(s)	1476
PstNI	CAGNNNCTG	2 cut(s)	505, 1471
PsuI	RGATCY	3 cut(s)	637, 914, 1025
PvuII	CAGCTG	2 cut(s)	104, 1781
RsaI	GTAC	9 cut(s)	19, 88, 347, 515, 625, 1188, 1245, 1258, 2236
RsaNI	GTAC	9 cut(s)	18, 87, 346, 514, 624, 1187, 1244, 1257, 2235
SacI	GAGCTC	1 cut(s)	683
SaqAI	TTAA	5 cut(s)	816, 1560, 2273, 2444, 2460
SatI	GCNGC	7 cut(s)	385, 1011, 1095, 1782, 2001, 2169, 2399
Sau3AI	GATC	6 cut(s)	256, 637, 914, 1025, 1141, 2106
Sau96I	GGNCC	4 cut(s)	993, 1503, 1831, 2308
SchI	GAGTC	3 cut(s)	401, 1327, 2147
ScrFI	CCNGG	8 cut(s)	117, 506, 1191, 1682, 1835, 2210, 2284, 2285
SduI	GDGCHC	1 cut(s)	683
SexAI	ACCWGGT	1 cut(s)	504
SfaNI	GCATC	5 cut(s)	510, 1392, 1682, 2173, 2410
SfcI	CTRYAG	4 cut(s)	1048, 1472, 1484, 1770
Sfr274I	CTCGAG	1 cut(s)	446
SinI	GGWCC	3 cut(s)	993, 1831, 2308
SlaI	CTCGAG	1 cut(s)	446
SmaI	CCCGGG	1 cut(s)	2285
SmlI	CTYRAG	5 cut(s)	446, 1736, 2069, 2117, 2158
SmoI	CTYRAG	5 cut(s)	446, 1736, 2069, 2117, 2158
SphI	GCATGC	2 cut(s)	270, 1661
SsiI	CCGC	8 cut(s)	200, 328, 384, 399, 991, 1589, 1963, 2311
SspMI	CTAG	7 cut(s)	378, 524, 617, 747, 978, 1451, 1710
SstI	GAGCTC	1 cut(s)	683
StyD4I	CCNGG	8 cut(s)	115, 504, 1189, 1680, 1833, 2208, 2282, 2283
StyI	CCWWGG	2 cut(s)	31, 996
TaaI	ACNGT	9 cut(s)	350, 814, 1186, 1243, 1366, 1376, 1691, 1767, 2260
TaqI	TCGA	2 cut(s)	447, 1792
TatI	WGTACW	3 cut(s)	86, 513, 623
TauI	GCSGC	1 cut(s)	387
TfiI	GAWTC	6 cut(s)	665, 689, 1166, 1307, 1775, 2147
Tru1I	TTAA	5 cut(s)	816, 1560, 2273, 2444, 2460
Tru9I	TTAA	5 cut(s)	816, 1560, 2273, 2444, 2460
TscAI	CASTG	5 cut(s)	178, 355, 1572, 1827, 2352
TseFI	GTSAC	1 cut(s)	599
TseI	GCWGC	6 cut(s)	1010, 1094, 1781, 2000, 2168, 2398
Tsp45I	GTSAC	1 cut(s)	599
TspMI	CCCGGG	1 cut(s)	2283
TspRI	CASTG	5 cut(s)	178, 355, 1572, 1827, 2352
VpaK11BI	GGWCC	3 cut(s)	993, 1831, 2308
XagI	CCTNNNNNAGG	3 cut(s)	1224, 1251, 2333
XapI	RAATTY	6 cut(s)	440, 757, 818, 826, 1595, 2278
XbaI	TCTAGA	1 cut(s)	377
XceI	RCATGY	4 cut(s)	162, 270, 1661, 2391
XhoI	CTCGAG	1 cut(s)	446
XmaI	CCCGGG	1 cut(s)	2283
XmiI	GTMKAC	1 cut(s)	1534
XspI	CTAG	7 cut(s)	378, 524, 617, 747, 978, 1451, 1710
Zsp2I	ATGCAT	1 cut(s)	1675

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.