Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002982

Physical Location & Seq

Genomic Coordinates Forward (+)

35520753 .. 35524413

Gene Structure 3661 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1272000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2760 bp

ATGTTTAAGTTTTTGCACCCACTGGGATCAAATTTTTGTGGGTTTTGTGAAATGGGAGTCGAGTTTCTGATGTTTTTGGAGAAATGGGTTGGTATTTCAACAGTTGGACTTGTGTGGCTGCATGCGTTATTGGATTTTAGCTGGGTTGCGCTTAATCTGTTTGTTTTCCAACTTTCTTACGATTTAGTGCTTCTAGGAGATGTTCTTTTTGTGCATATTTGTTCTCGCTTAGTAGCTCAGAGTATGAAAAAAATGGTCAACAGTGGTACCGGAAAAGGCAGTAAGAACAAGAGAAAAGCTGACCCTGCCCCGCAGAATGCAACTAACCCTGGTTTGCCCCTAACCGTAACCCCTCAGAGCGAATTACCTTCAGAGGAATGTAGAAATTTGAGTGACAAGGTGTTAGCTCAGGCGGGGGTGGTTCAGCCAGAAGAAGTGGAAGTGGGAGCTTTTGAGTGTGCAGATTGGGATGACCCAATTGCTTGCCAGCTTGAAGAACTACTACTGTCTTATTTGCAAGCAATTTTCCGGAGTGCTATTAAGCAGGTTGCTGAACATGGGTACAGTGAGGAGGTTGCTGAAAAGGCTGTTTCGAGAGGCGGCCTTTACATTGGCGGTAAAGACCCTCTAGCAAATATTCACCTGGTGGAATACACAATGCTAGAAATGATCAGTGTGGTTAGGGAGGTAAGGCTATCCTTATCAGTTGCGGAAGCAATGTGGTGGCTGTTGATATGTGACTTAAACATTTTGCTAGCCTGCACGTCGGAAGGAGATCCATTTAGTGCTCTTGGTTCTGAAGAATCCGCTGATTCGTCTTCTCCCCAATTAAGCTCAGAGGCAAATAGCTCTGAAACCATTCTTCCAAATCCCAATGAAGCAAATTCCTCAAAGCCACTGCCTGCCTATGCTCAAAGCAATCCTCCTGAAACACTCAAATTTGGAAGTTTCCGTATACATTCCAACAGCACACATTCTCATGCACCTGAAGAGGTAACCCCTGAGAAAGATAGTTTGTCATCTATGCTAGATAGTCTGGAAAAGTGCTTAGGATTCACTGGAGAAAATGTCCAATCAAAATCTCAGACATCCACCTCTGAAGAAAAGTCAGGAGCTGGTCTAACAGGGCGCACCAAGAAAGAATTAGCAGCACTTCGTAAGAAGTCCTTCCATACGGATCGAAATTACAGCGCTTATGGGAAAAAAGGAGGCTTCAAATCAGGGAAAGTCACGATTGGTGGTTTTGTTCTGGAGAAGAGGCTAAAGCCTCCATCTGAAATTCCCGGAGGACGAGAAACAGTTGCATCCTCAAAGTTGAGTGCCAAAGCTGGATCCACCATCCCTTCTTCAGATGGAAGACATAGGGCTTCAACCAAGATTTCATCTGCTTCACTAGCAACAGGTGGCACAGTAACATTACCTATATCTTACTTGCGATCCGCAGATCCCAATCTTTCCCAGAAGTCTTGTTTGGAAAACAAATCTAGACCTAAGGCCAAGATCCCGGTTGGCACCTCTCCATCCCCCAAGACTCATGATTGCTGTGCTGGCTTGCCATGTGATGACAAGTCTCTAGGAAAGAATATCCCAAAAGATGAGATCGATGAGCTGTTTTTGAAGGTAGCTCCTCGGCTAAAGGATCTTGTTCCTCTTGATTACCGTGCTGGCATCCCATATGATAAGTCTCTAGGAAAGTATGTCGCACAAGATGAGAAGGATGAGCTGATTTTAAAACTAGTACCTCGGCTGGAGGAGCTGCAGAATGAACTAGAAAGCTGGACTAATTGGGCCAACGAGAAGATTATGCAGGTTTCTCGTAGGCTTAGTAAGGAACAACCTGAACTTAAAATACTGAGGCAAGAGAAGGCAGAAGCAGAGAAATTTAAAAAAGAGAAACAAATGTTGGAGGAGAACACTGTGAAGAGAATCTCTGAGTTGGAGAATGCCTTAAATAATGCAACTGACCAGGTTGAGAAAGCTACCTCTAATATCTGTAGGCTTGAGTTGGAGAATTCCACTCTTAAAGAGGAGAGGAGTACTGCCGAAAGAATGGCTATAGATTCGACTGCACGTCACCAGGAAGCATTAGAGAGGGAGCAGAATGCACTGAAAAAGGTCCAGGCATGGGAGGGGCAGAGGAGCGCATTGCAGGAAGAGCTTGAGACAGGAAAGAAGAAGGTGGCTGCACTGCAACAGGATTTAGCCAAGGCAAAAAATGTCCATCATCAAATCGAGGCTAGATGGAAAAGGGAGAAGATAGAAAGGGAGAAAATACTTGTGCAGGCCGACTTCATAAGAAAAGAAAGAGAACAACGTCAAGCTTTGACAAAATTGGAGGAGGACAGGATCAAACTGAGCGCAGAATATGATATGCGGGAGTATATGGAAGACATCAGAAAAGTTGAGAGCAAGTTATCAGAACTTAAGTTGAAGTCCGACTCCTCGAGAATTGCAGCACTAAGAAGGGGAGCTGCCGGAAGCTTTGGAGGTTCCCCGTCAGACAGAAAAAGTGGGAAGGCAACAAAGGGAAACCAAAACTTTACCAGCCTCAAGAAGGTAAAGAATTCCCAGGACTTGAGACAGGACCGCGAATGTGTGATGTGCCTATCTGAGGAGATGTCGGTGGTGTTCCTTCCGTGTGCACATCAGGTTGTTTGTGCAAATTGCAATGAGCTTCACAAGAAACAAGGAATGAAGGATTGCCCTTCTTGTAGGACCCCGATTCAGCGGAGGATCAACGTGCAGTATGCTCATCCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009987 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

920

Amino Acids

102.1

Weight (kDa)

7.91

Isoelectric Point (pI)

49.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	862 - 910	2.4e-12	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002982)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03365 AT1G03365 AT4G03000 AT4G03000 AT4G03000 AT4G03000 AT4G03000 AT4G03000
fragaria_vesca	FvH4_2g24221 FvH4_2g24221 FvH4_2g24221
malus_domestica	MD03G1272000.v1.1 MD11G1292600.v1.1
prunus_persica	Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1 Prupe.8G248200_v2.0.a1
pyrus_communis	pycom03g21660 pycom11g25900
rosa_chinensis	RchiOBHm_Chr6g0292011
rosa_laevigata	RLG00000012070
rosa_multiflora	Rmu_co8265653.1_g000001 Rmu_co8382791.1_g000001 Rmu_sc0008666.1_g000003
rosa_roxburghii	Rroxscaffold_7G00175490 Rroxscaffold_7G00178030
rosa_rugosa	Rorug06G0222500 Rorug06G0222600 Rorug06G0222700 Rorug06G0222800 Rorug06G0222900
rosa_samantha	Rh6AG336400 Rh6BG343700 Rh6CG350000 Rh6DG336700
rosa_wichuraiana	Rw6G029280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	535, 1798
Acc65I	GGTACC	1 cut(s)	266
AccB1I	GGYRCC	2 cut(s)	266, 1511
AccI	GTMKAC	1 cut(s)	955
AccII	CGCG	1 cut(s)	2589
AccIII	TCCGGA	1 cut(s)	528
AcsI	RAATTY	8 cut(s)	31, 385, 883, 938, 1278, 1880, 2011, 2563
AcuI	CTGAAG	5 cut(s)	354, 819, 1008, 1119, 1332
AdeI	CACNNNGTG	1 cut(s)	646
AfaI	GTAC	4 cut(s)	268, 563, 1740, 2038
AfeI	AGCGCT	1 cut(s)	1192
AfiI	CCNNNNNNNGG	3 cut(s)	2125, 2554, 2611
AflII	CTTAAG	1 cut(s)	2423
AgsI	TTSAA	6 cut(s)	99, 494, 1216, 1371, 1618, 2431
AhdI	GACNNNNNGTC	1 cut(s)	2070
AhlI	ACTAGT	1 cut(s)	1735
AjiI	CACGTC	2 cut(s)	765, 2072
AjnI	CCWGG	6 cut(s)	328, 642, 1965, 2076, 2118, 2568
AjuI	GAANNNNNNNTTGG	4 cut(s)	1454, 1486, 1886, 1918
Alw21I	GWGCWC	2 cut(s)	790, 2644
Alw26I	GTCTC	4 cut(s)	1575, 1689, 2156, 2572
Alw44I	GTGCAC	1 cut(s)	2640
AlwNI	CAGNNNCTG	1 cut(s)	1115
Ama87I	CYCGRG	1 cut(s)	2443
Aor13HI	TCCGGA	1 cut(s)	528
Aor51HI	AGCGCT	1 cut(s)	1192
AoxI	GGCC	4 cut(s)	601, 1494, 1788, 2283
ApaLI	GTGCAC	1 cut(s)	2640
ApeKI	GCWGC	6 cut(s)	118, 1148, 1756, 2183, 2453, 2471
ApoI	RAATTY	8 cut(s)	31, 385, 883, 938, 1278, 1880, 2011, 2563
Asp700I	GAANNNNTTC	2 cut(s)	858, 1166
Asp718I	GGTACC	1 cut(s)	266
AspLEI	GCGC	5 cut(s)	151, 1131, 1193, 2144, 2360
AspS9I	GGNCC	4 cut(s)	1788, 2116, 2584, 2715
AsuC2I	CCSGG	2 cut(s)	1284, 1505
AsuHPI	GGTGA	2 cut(s)	632, 2066
AsuNHI	GCTAGC	1 cut(s)	754
AvaI	CYCGRG	1 cut(s)	2443
AvaII	GGWCC	3 cut(s)	2116, 2584, 2715
AxyI	CCTNAGG	1 cut(s)	1491
BaeGI	GKGCMC	1 cut(s)	2644
BaeI	ACNNNNGTAYC	2 cut(s)	250, 283
BamHI	GGATCC	1 cut(s)	1331
BanI	GGYRCC	2 cut(s)	266, 1511
BarI	GAAGNNNNNNTAC	2 cut(s)	486, 518
BbsI	GAAGAC	3 cut(s)	810, 1363, 2394
Bbv12I	GWGCWC	2 cut(s)	790, 2644
BbvI	GCAGC	6 cut(s)	105, 1160, 1743, 2170, 2458, 2465
BccI	CCATC	6 cut(s)	1279, 1346, 1346, 1528, 2229, 2235
BcgI	CGANNNNNNTGC	2 cut(s)	1796, 1830
BciT130I	CCWGG	6 cut(s)	330, 644, 1967, 2078, 2120, 2570
BclI	TGATCA	1 cut(s)	669
BcnI	CCSGG	2 cut(s)	1284, 1505
BcoDI	GTCTC	4 cut(s)	1575, 1689, 2156, 2572
BcuI	ACTAGT	1 cut(s)	1735
BfmI	CTRYAG	3 cut(s)	1757, 1993, 2055
BfoI	RGCGCY	1 cut(s)	1194
BfrI	CTTAAG	1 cut(s)	2423
BfuAI	ACCTGC	2 cut(s)	535, 1798
BisI	GCNGC	7 cut(s)	119, 601, 1149, 1757, 2184, 2454, 2472
BlsI	GCNGC	7 cut(s)	120, 602, 1150, 1758, 2185, 2455, 2473
BmcAI	AGTACT	1 cut(s)	2038
Bme1390I	CCNGG	8 cut(s)	330, 644, 1284, 1505, 1967, 2078, 2120, 2570
Bme18I	GGWCC	3 cut(s)	2116, 2584, 2715
BmeRI	GACNNNNNGTC	1 cut(s)	2070
BmeT110I	CYCGRG	1 cut(s)	2443
BmgBI	CACGTC	2 cut(s)	765, 2072
BmgT120I	GGNCC	4 cut(s)	1788, 2116, 2584, 2715
BmiI	GGNNCC	5 cut(s)	268, 1333, 1513, 2491, 2717
BmrFI	CCNGG	8 cut(s)	330, 644, 1284, 1505, 1967, 2078, 2120, 2570
BmrI	ACTGGG	1 cut(s)	32
BmsI	GCATC	2 cut(s)	1313, 1677
BmtI	GCTAGC	1 cut(s)	758
BmuI	ACTGGG	1 cut(s)	32
BpiI	GAAGAC	3 cut(s)	810, 1363, 2394
BpmI	CTGGAG	3 cut(s)	1080, 1271, 1769
Bpu10I	CCTNAGC	2 cut(s)	408, 1048
BpuEI	CTTGAG	4 cut(s)	2021, 2180, 2534, 2596
BpuMI	CCSGG	2 cut(s)	1284, 1505
Bsa29I	ATCGAT	1 cut(s)	1602
BsaBI	GATNNNNATC	1 cut(s)	1449
BsaJI	CCNNGG	5 cut(s)	328, 1628, 1742, 2205, 2568
BsaWI	WCCGGW	2 cut(s)	269, 528
BsaXI	ACNNNNNCTCC	2 cut(s)	1053, 1083
Bsc4I	CCNNNNNNNGG	3 cut(s)	2125, 2554, 2611
Bse1I	ACTGG	2 cut(s)	27, 1063
Bse21I	CCTNAGG	1 cut(s)	1491
Bse3DI	GCAATG	3 cut(s)	723, 2144, 2674
Bse8I	GATNNNNATC	1 cut(s)	1449
BseAI	TCCGGA	1 cut(s)	528
BseBI	CCWGG	6 cut(s)	330, 644, 1967, 2078, 2120, 2570
BseCI	ATCGAT	1 cut(s)	1602
BseDI	CCNNGG	5 cut(s)	328, 1628, 1742, 2205, 2568
BseGI	GGATG	8 cut(s)	475, 1088, 1304, 1338, 1520, 1668, 1723, 2752
BseJI	GATNNNNATC	1 cut(s)	1449
BseLI	CCNNNNNNNGG	3 cut(s)	2125, 2554, 2611
BseMI	GCAATG	3 cut(s)	723, 2144, 2674
BseNI	ACTGG	2 cut(s)	27, 1063
BseSI	GKGCMC	1 cut(s)	2644
BseXI	GCAGC	6 cut(s)	105, 1160, 1743, 2170, 2458, 2465
BseYI	CCCAGC	1 cut(s)	141
BsgI	GTGCAG	5 cut(s)	480, 745, 2052, 2169, 2300
Bsh1236I	CGCG	1 cut(s)	2589
BshFI	GGCC	4 cut(s)	603, 1496, 1790, 2285
BshNI	GGYRCC	2 cut(s)	266, 1511
BshVI	ATCGAT	1 cut(s)	1602
BsiHKAI	GWGCWC	2 cut(s)	790, 2644
BsiHKCI	CYCGRG	1 cut(s)	2443
BsiSI	CCGG	5 cut(s)	270, 529, 1284, 1505, 2475
BslI	CCNNNNNNNGG	3 cut(s)	2125, 2554, 2611
BsmAI	GTCTC	4 cut(s)	1575, 1689, 2156, 2572
BsmI	GAATGC	3 cut(s)	322, 1948, 2107
BsnI	GGCC	4 cut(s)	603, 1496, 1790, 2285
BsoBI	CYCGRG	1 cut(s)	2443
Bsp1286I	GDGCHC	2 cut(s)	790, 2644
Bsp13I	TCCGGA	1 cut(s)	528
BspANI	GGCC	4 cut(s)	603, 1496, 1790, 2285
BspDI	ATCGAT	1 cut(s)	1602
BspEI	TCCGGA	1 cut(s)	528
BspFNI	CGCG	1 cut(s)	2589
BspHI	TCATGA	1 cut(s)	1534
BspLI	GGNNCC	5 cut(s)	268, 1333, 1513, 2491, 2717
BspMAI	CTGCAG	1 cut(s)	1761
BspMI	ACCTGC	2 cut(s)	535, 1798
BspOI	GCTAGC	1 cut(s)	758
BspQI	GCTCTTC	1 cut(s)	2148
BspT107I	GGYRCC	2 cut(s)	266, 1511
BspTI	CTTAAG	1 cut(s)	2423
BsrDI	GCAATG	3 cut(s)	723, 2144, 2674
BsrI	ACTGG	2 cut(s)	27, 1063
BssECI	CCNNGG	5 cut(s)	328, 1628, 1742, 2205, 2568
BssNAI	GTATAC	1 cut(s)	956
BssT1I	CCWWGG	1 cut(s)	2205
Bst1107I	GTATAC	1 cut(s)	956
Bst2UI	CCWGG	6 cut(s)	330, 644, 1967, 2078, 2120, 2570
Bst4CI	ACNGT	9 cut(s)	103, 263, 346, 507, 566, 1300, 1411, 1661, 1918
Bst6I	CTCTTC	4 cut(s)	984, 1250, 1916, 2148
BstAFI	CTTAAG	1 cut(s)	2423
BstEII	GGTNACC	1 cut(s)	994
BstENI	CCTNNNNNAGG	2 cut(s)	2552, 2609
BstF5I	GGATG	8 cut(s)	475, 1088, 1304, 1338, 1520, 1668, 1723, 2752
BstFNI	CGCG	1 cut(s)	2589
BstH2I	RGCGCY	1 cut(s)	1194
BstHHI	GCGC	5 cut(s)	151, 1131, 1193, 2144, 2360
BstMAI	GTCTC	4 cut(s)	1575, 1689, 2156, 2572
BstMWI	GCNNNNNNNGC	6 cut(s)	305, 584, 1394, 1548, 1753, 2155
BstNI	CCWGG	6 cut(s)	330, 644, 1967, 2078, 2120, 2570
BstNSI	RCATGY	1 cut(s)	125
BstPI	GGTNACC	1 cut(s)	994
BstSCI	CCNGG	8 cut(s)	328, 642, 1282, 1503, 1965, 2076, 2118, 2568
BstSFI	CTRYAG	3 cut(s)	1757, 1993, 2055
BstSLI	GKGCMC	1 cut(s)	2644
BstUI	CGCG	1 cut(s)	2589
BstV1I	GCAGC	6 cut(s)	105, 1160, 1743, 2170, 2458, 2465
BstV2I	GAAGAC	3 cut(s)	810, 1363, 2394
BstX2I	RGATCY	5 cut(s)	775, 1331, 1444, 1500, 1639
BstYI	RGATCY	5 cut(s)	775, 1331, 1444, 1500, 1639
BstZ17I	GTATAC	1 cut(s)	956
Bsu15I	ATCGAT	1 cut(s)	1602
Bsu36I	CCTNAGG	1 cut(s)	1491
BsuRI	GGCC	4 cut(s)	603, 1496, 1790, 2285
BsuTUI	ATCGAT	1 cut(s)	1602
BtrI	CACGTC	2 cut(s)	765, 2072
BtsCI	GGATG	8 cut(s)	475, 1088, 1304, 1338, 1520, 1668, 1723, 2752
BtsI	GCAGTG	2 cut(s)	896, 2186
BtsIMutI	CAGTG	9 cut(s)	20, 268, 571, 679, 896, 1056, 1914, 2105, 2186
BveI	ACCTGC	2 cut(s)	535, 1798
CaiI	CAGNNNCTG	1 cut(s)	1115
CciI	TCATGA	1 cut(s)	1534
CfoI	GCGC	5 cut(s)	151, 1131, 1193, 2144, 2360
Cfr13I	GGNCC	4 cut(s)	1788, 2116, 2584, 2715
ClaI	ATCGAT	1 cut(s)	1602
CsiI	ACCWGGT	2 cut(s)	642, 1965
Csp6I	GTAC	4 cut(s)	267, 562, 1739, 2037
CviAII	CATG	6 cut(s)	122, 557, 980, 1535, 1557, 2124
CviQI	GTAC	4 cut(s)	267, 562, 1739, 2037
DraI	TTTAAA	2 cut(s)	1731, 1885
DraIII	CACNNNGTG	1 cut(s)	646
DriI	GACNNNNNGTC	1 cut(s)	2070
Eam1104I	CTCTTC	4 cut(s)	984, 1250, 1916, 2148
Eam1105I	GACNNNNNGTC	1 cut(s)	2070
EarI	CTCTTC	4 cut(s)	984, 1250, 1916, 2148
Eco130I	CCWWGG	1 cut(s)	2205
Eco47I	GGWCC	3 cut(s)	2116, 2584, 2715
Eco47III	AGCGCT	1 cut(s)	1192
Eco57I	CTGAAG	5 cut(s)	354, 819, 1008, 1119, 1332
Eco81I	CCTNAGG	1 cut(s)	1491
Eco88I	CYCGRG	1 cut(s)	2443
Eco91I	GGTNACC	1 cut(s)	994
EcoNI	CCTNNNNNAGG	2 cut(s)	2552, 2609
EcoO109I	RGGNCCY	1 cut(s)	2715
EcoO65I	GGTNACC	1 cut(s)	994
EcoRI	GAATTC	2 cut(s)	2011, 2563
EcoRII	CCWGG	6 cut(s)	328, 642, 1965, 2076, 2118, 2568
EcoT14I	CCWWGG	1 cut(s)	2205
ErhI	CCWWGG	1 cut(s)	2205
FaeI	CATG	6 cut(s)	125, 560, 983, 1538, 1560, 2127
FalI	AAGNNNNNCTT	2 cut(s)	1151, 1183
FatI	CATG	6 cut(s)	121, 556, 979, 1534, 1556, 2123
FauI	CCCGC	3 cut(s)	318, 406, 2367
FauNDI	CATATG	1 cut(s)	1675
FbaI	TGATCA	1 cut(s)	669
FblI	GTMKAC	1 cut(s)	955
Fnu4HI	GCNGC	7 cut(s)	119, 601, 1149, 1757, 2184, 2454, 2472
FokI	GGATG	8 cut(s)	482, 1075, 1291, 1325, 1507, 1655, 1730, 2739
Fsp4HI	GCNGC	7 cut(s)	119, 601, 1149, 1757, 2184, 2454, 2472
GlaI	GCGC	5 cut(s)	150, 1130, 1192, 2143, 2359
GluI	GCNGC	7 cut(s)	119, 601, 1149, 1757, 2184, 2454, 2472
GsaI	CCCAGC	1 cut(s)	145
GsuI	CTGGAG	3 cut(s)	1080, 1271, 1769
HaeII	RGCGCY	1 cut(s)	1194
HaeIII	GGCC	4 cut(s)	603, 1496, 1790, 2285
HapII	CCGG	5 cut(s)	270, 529, 1284, 1505, 2475
HhaI	GCGC	5 cut(s)	151, 1131, 1193, 2144, 2360
Hin1II	CATG	6 cut(s)	125, 560, 983, 1538, 1560, 2127
Hin6I	GCGC	5 cut(s)	149, 1129, 1191, 2142, 2358
HinP1I	GCGC	5 cut(s)	149, 1129, 1191, 2142, 2358
HincII	GTYRAC	1 cut(s)	259
HindII	GTYRAC	1 cut(s)	259
HindIII	AAGCTT	2 cut(s)	2319, 2479
HinfI	GANTC	9 cut(s)	57, 803, 812, 1053, 1531, 1926, 2060, 2438, 2722
HpaII	CCGG	5 cut(s)	270, 529, 1284, 1505, 2475
HphI	GGTGA	2 cut(s)	632, 2066
Hpy166II	GTNNAC	3 cut(s)	259, 956, 2642
Hpy8I	GTNNAC	3 cut(s)	259, 956, 2642
Hpy99I	CGWCG	1 cut(s)	769
HpyCH4III	ACNGT	9 cut(s)	103, 263, 346, 507, 566, 1300, 1411, 1661, 1918
HpyCH4IV	ACGT	4 cut(s)	764, 2071, 2314, 2739
HpyF10VI	GCNNNNNNNGC	6 cut(s)	305, 584, 1394, 1548, 1753, 2155
HpySE526I	ACGT	4 cut(s)	764, 2071, 2314, 2739
Hsp92II	CATG	6 cut(s)	125, 560, 983, 1538, 1560, 2127
HspAI	GCGC	5 cut(s)	149, 1129, 1191, 2142, 2358
Kpn2I	TCCGGA	1 cut(s)	528
KpnI	GGTACC	1 cut(s)	270
Ksp22I	TGATCA	1 cut(s)	669
LguI	GCTCTTC	1 cut(s)	2148
LmnI	GCTCC	7 cut(s)	446, 1112, 1630, 1753, 2095, 2139, 2468
Lsp1109I	GCAGC	6 cut(s)	105, 1160, 1743, 2170, 2458, 2465
LweI	GCATC	2 cut(s)	1313, 1677
MabI	ACCWGGT	2 cut(s)	642, 1965
MaeII	ACGT	4 cut(s)	764, 2071, 2314, 2739
MaeIII	GTNAC	7 cut(s)	346, 392, 737, 994, 1228, 1411, 2072
MfeI	CAATTG	1 cut(s)	477
MflI	RGATCY	5 cut(s)	775, 1331, 1444, 1500, 1639
MhlI	GDGCHC	2 cut(s)	790, 2644
MlyI	GAGTC	3 cut(s)	66, 1525, 2432
MmeI	TCCRAC	8 cut(s)	85, 193, 747, 987, 1884, 1917, 1986, 2460
MroI	TCCGGA	1 cut(s)	528
MroXI	GAANNNNTTC	2 cut(s)	858, 1166
MslI	CAYNNNNRTG	1 cut(s)	978
MspA1I	CMGCKG	2 cut(s)	809, 2728
MspCI	CTTAAG	1 cut(s)	2423
MspI	CCGG	5 cut(s)	270, 529, 1284, 1505, 2475
MspR9I	CCNGG	8 cut(s)	330, 644, 1284, 1505, 1967, 2078, 2120, 2570
MunI	CAATTG	1 cut(s)	477
Mva1269I	GAATGC	3 cut(s)	322, 1948, 2107
MvaI	CCWGG	6 cut(s)	330, 644, 1967, 2078, 2120, 2570
MvnI	CGCG	1 cut(s)	2589
MwoI	GCNNNNNNNGC	6 cut(s)	305, 584, 1394, 1548, 1753, 2155
NciI	CCSGG	2 cut(s)	1284, 1505
NdeI	CATATG	1 cut(s)	1675
NheI	GCTAGC	1 cut(s)	754
NlaIII	CATG	6 cut(s)	125, 560, 983, 1538, 1560, 2127
NlaIV	GGNNCC	5 cut(s)	268, 1333, 1513, 2491, 2717
NmeAIII	GCCGAG	2 cut(s)	1609, 1723
NmuCI	GTSAC	4 cut(s)	392, 737, 1228, 2072
NspI	RCATGY	1 cut(s)	125
PaeI	GCATGC	1 cut(s)	125
PaeR7I	CTCGAG	1 cut(s)	2443
PagI	TCATGA	1 cut(s)	1534
PciSI	GCTCTTC	1 cut(s)	2148
PctI	GAATGC	3 cut(s)	322, 1948, 2107
PdmI	GAANNNNTTC	2 cut(s)	858, 1166
PfeI	GAWTC	6 cut(s)	803, 812, 1053, 1926, 2060, 2722
PfoI	TCCNGGA	1 cut(s)	1282
PkrI	GCNGC	7 cut(s)	120, 602, 1150, 1758, 2185, 2455, 2473
PleI	GAGTC	3 cut(s)	65, 1525, 2432
PpsI	GAGTC	3 cut(s)	65, 1525, 2432
PpuMI	RGGWCCY	1 cut(s)	2715
Psp5II	RGGWCCY	1 cut(s)	2715
Psp6I	CCWGG	6 cut(s)	328, 642, 1965, 2076, 2118, 2568
PspEI	GGTNACC	1 cut(s)	994
PspFI	CCCAGC	1 cut(s)	141
PspGI	CCWGG	6 cut(s)	328, 642, 1965, 2076, 2118, 2568
PspN4I	GGNNCC	5 cut(s)	268, 1333, 1513, 2491, 2717
PspPI	GGNCC	4 cut(s)	1788, 2116, 2584, 2715
PspPPI	RGGWCCY	1 cut(s)	2715
PsrI	GAACNNNNNNTAC	2 cut(s)	1833, 1865
PstI	CTGCAG	1 cut(s)	1761
PstNI	CAGNNNCTG	1 cut(s)	1115
PsuI	RGATCY	5 cut(s)	775, 1331, 1444, 1500, 1639
RsaI	GTAC	4 cut(s)	268, 563, 1740, 2038
RsaNI	GTAC	4 cut(s)	267, 562, 1739, 2037
RseI	CAYNNNNRTG	1 cut(s)	978
SapI	GCTCTTC	1 cut(s)	2148
SatI	GCNGC	7 cut(s)	119, 601, 1149, 1757, 2184, 2454, 2472
Sau96I	GGNCC	4 cut(s)	1788, 2116, 2584, 2715
ScaI	AGTACT	1 cut(s)	2038
SchI	GAGTC	3 cut(s)	66, 1525, 2432
ScrFI	CCNGG	8 cut(s)	330, 644, 1284, 1505, 1967, 2078, 2120, 2570
SduI	GDGCHC	2 cut(s)	790, 2644
SexAI	ACCWGGT	2 cut(s)	642, 1965
SfaNI	GCATC	2 cut(s)	1313, 1677
SfcI	CTRYAG	3 cut(s)	1757, 1993, 2055
Sfr274I	CTCGAG	1 cut(s)	2443
SinI	GGWCC	3 cut(s)	2116, 2584, 2715
SlaI	CTCGAG	1 cut(s)	2443
SmiMI	CAYNNNNRTG	1 cut(s)	978
SmlI	CTYRAG	6 cut(s)	2000, 2159, 2423, 2443, 2549, 2575
SmoI	CTYRAG	6 cut(s)	2000, 2159, 2423, 2443, 2549, 2575
SpeI	ACTAGT	1 cut(s)	1735
SphI	GCATGC	1 cut(s)	125
SspI	AATATT	1 cut(s)	637
StyD4I	CCNGG	8 cut(s)	328, 642, 1282, 1503, 1965, 2076, 2118, 2568
StyI	CCWWGG	1 cut(s)	2205
TaaI	ACNGT	9 cut(s)	103, 263, 346, 507, 566, 1300, 1411, 1661, 1918
TaiI	ACGT	4 cut(s)	767, 2074, 2317, 2742
TaqI	TCGA	7 cut(s)	60, 593, 1180, 1602, 2063, 2232, 2444
TatI	WGTACW	1 cut(s)	2036
TauI	GCSGC	1 cut(s)	603
TfiI	GAWTC	6 cut(s)	803, 812, 1053, 1926, 2060, 2722
TscAI	CASTG	9 cut(s)	27, 268, 571, 679, 903, 1063, 1921, 2112, 2193
TseFI	GTSAC	4 cut(s)	392, 737, 1228, 2072
TseI	GCWGC	6 cut(s)	118, 1148, 1756, 2183, 2453, 2471
Tsp45I	GTSAC	4 cut(s)	392, 737, 1228, 2072
TspDTI	ATGAA	6 cut(s)	260, 891, 1371, 1779, 2281, 2708
TspGWI	ACGGA	3 cut(s)	941, 1190, 2625
TspRI	CASTG	9 cut(s)	27, 268, 571, 679, 903, 1063, 1921, 2112, 2193
Vha464I	CTTAAG	1 cut(s)	2423
VneI	GTGCAC	1 cut(s)	2640
VpaK11BI	GGWCC	3 cut(s)	2116, 2584, 2715
XagI	CCTNNNNNAGG	2 cut(s)	2552, 2609
XapI	RAATTY	8 cut(s)	31, 385, 883, 938, 1278, 1880, 2011, 2563
XbaI	TCTAGA	1 cut(s)	1484
XceI	RCATGY	1 cut(s)	125
XhoI	CTCGAG	1 cut(s)	2443
XmiI	GTMKAC	1 cut(s)	955
XmnI	GAANNNNTTC	2 cut(s)	858, 1166
ZrmI	AGTACT	1 cut(s)	2038

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.