FvH4_2g24350

LEC14B protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001751
Physical Location & Seq
Reverse (-)
19895782 .. 19900389
4608 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24350.t1

Sequence Viewer

Length: 1476 bp
ATGCTTGTTACTTCAATTGATGATTTCGATGAGATGGGGTATTCACTGAGTAGATTGACTGTAGAGTCTGACTATTTTGATGATGGGAGTACCAATAACGGAACTAGTGAAGGACCCGACAAAGCTTTTGAGAGTTTAGAGCATGAAGTAGCTCAGCTCACAAAGCTGAGATCAGCACCCAATGAGAGATTGAGACAAGTCGGACCGGGGAGGCGGGGCAGGACTGTTTCGACGGTGAAGATGCTGGAAGGGAGAGAGGGTAATTATTCAGGAAGGGGGAGATTCTCCTCTGCTGATTGTTGTCATGTTTCAGGGAGATACTTGCCGCTGAATGGTCCTTGGCTTATTGACCTATTGCCTTGCCGAGCATATGTCTCGCAGTTTTCAGCAGATGGATCTCTTTTTATTGCTGGGTTTCAGGGTAGCCACATTAAAATATACAATGTGGATAAAGGGTGGAAAGTGCAGAAGGACATTCATGCCAAAAATCTGCGATGGACAGTGACTGATACATCTCTTTCCCCGGATCAACGCCATCTTGTCTATGCAAGCATGTCACCCATTGTTCATATAGTTAATCTGGGATCTGCAGAAACAGAATCTCGTGCTAATATAACGGAAATTCATGAGGGTTTGGATTTTACATCTAGTGATGATCGAGGATACTCCTTCGGTATTTTCTCCATAAAATTTTCAACAGATGGACGTGAAATTGTTGCTGGAAGTAGTGATGATTCCATATATGTTTATGATCTTGAAACTAATAAGCTCTCCCTTCGAATTTTGGCTCACACGTCTGATGTGAACACTGTTTGTTTTGCCGATGAAAGTGGCCATCTTATTTATTCTGGGAGTGATGATACTCTCTGTAAGGTGTGGGATAGACGTTGCTTTCAAGCCAGCGGGAAGCCAGCAGGAGTTTTGACAGGGCACCTAGAAGGTATTACATGCATTGACAGCAAAGGAGACGGACGTTACTTCATTTCAAATAGCAAAGATCAGAGCATAAAACTTTGGGATATCAGGAAAATGTGCTCTAGTGCTACTGCTGCGCGCAACCCAGGGCCTAGATATCATGAATGGGATTACAGATGGATGGACTACCCGCCTCAGGCAAAAGATATGAAACATCCATCTGATGTATCGGTGTCTACTTATAAAGGTCATTCGGTCTTGCGTACACTTATTCGCTCCTACTTCTCCCCAGCGTATAGCAGTGGTCAGAAGTACATCTACACTGGATCTCACAATTCATGCGTTTATATATATGATTTGGTGAGTGGAAAAGAAGTTGGAAAACTGGAGCATCATAAATCAGCTGTAAGAGATTGTAGTTGGCACCCATATTACCCTATGCTTGTCAGCTCTTCCTGGGATGGGGAGGTTGTTAGATGGGAATTCACTGGAAATGGAGACACACCACCACCTTTCACCAGAAGGGGAGCCTGGAGGAGAGAATATTCACCATTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

492

Amino Acids

55.2

Weight (kDa)

6.39

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 127 - 298 7.2e-17 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 138 - 290 1.4e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 142 - 294 4.1e-14 WDR3 first beta-propeller domain
WDR55 PF24796 167 - 297 2.4e-12 WDR55
WD40_MABP1-WDR62_2nd PF24782 212 - 294 2.1e-06 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 220 - 299 7e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 221 - 340 1.7e-15 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 224 - 343 1.5e-21 WDR5 beta-propeller domain
WD40_Prp19 PF24814 225 - 344 3.4e-16 Prp19 WD40 domain
Beta-prop_EML PF23409 226 - 330 8.1e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz PF24807 226 - 347 4e-14 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 226 - 351 2.5e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 226 - 349 2.8e-20 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 228 - 448 4e-16 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 228 - 352 2.4e-19 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 237 - 344 6.2e-11 TEP-1 second beta-propeller
EIF3I PF24805 264 - 460 3.1e-13 EIF3I
WD40 PF00400 302 - 340 1.5e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 382 - 464 5.1e-10 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 403 - 468 2.9e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 403 - 468 9.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 403 - 467 1.2e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 407 - 469 4.2e-07 Prp19 WD40 domain
WD40 PF00400 428 - 465 2e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1158
AasI GACNNNNNNGTC 1 cut(s) 64
AccB1I GGYRCC 2 cut(s) 930, 1338
AccI GTMKAC 1 cut(s) 1151
AccII CGCG 1 cut(s) 1054
AciI CCGC 4 cut(s) 214, 326, 903, 1106
AclWI GGATC 4 cut(s) 403, 534, 592, 1249
AcoI YGGCCR 1 cut(s) 832
AcsI RAATTY 4 cut(s) 621, 689, 780, 1397
AfaI GTAC 3 cut(s) 91, 1180, 1229
AfiI CCNNNNNNNGG 3 cut(s) 332, 1111, 1377
AflIII ACRYGT 1 cut(s) 792
AgsI TTSAA 5 cut(s) 15, 696, 758, 896, 987
AhlI ACTAGT 1 cut(s) 104
AjiI CACGTC 2 cut(s) 707, 795
AjnI CCWGG 3 cut(s) 1060, 1370, 1445
AluBI AGCT 7 cut(s) 125, 152, 157, 166, 769, 1319, 1365
AluI AGCT 7 cut(s) 125, 152, 157, 166, 769, 1319, 1365
Alw21I GWGCWC 1 cut(s) 1037
Alw26I GTCTC 4 cut(s) 187, 379, 960, 1407
AlwI GGATC 4 cut(s) 403, 534, 592, 1249
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 2 cut(s) 832, 1064
ApeKI GCWGC 1 cut(s) 1049
ApoI RAATTY 4 cut(s) 621, 689, 780, 1397
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AspLEI GCGC 2 cut(s) 1054, 1056
AspS9I GGNCC 4 cut(s) 113, 203, 335, 1064
AsuC2I CCSGG 2 cut(s) 207, 524
AsuHPI GGTGA 5 cut(s) 247, 549, 1288, 1423, 1455
AsuII TTCGAA 1 cut(s) 778
AvaII GGWCC 3 cut(s) 113, 203, 335
AxyI CCTNAGG 1 cut(s) 1110
BaeGI GKGCMC 1 cut(s) 933
BalI TGGCCA 1 cut(s) 834
BanI GGYRCC 2 cut(s) 930, 1338
BarI GAAGNNNNNNTAC 2 cut(s) 1217, 1249
BauI CACGAG 1 cut(s) 603
Bbv12I GWGCWC 1 cut(s) 1037
BbvI GCAGC 1 cut(s) 1036
BcgI CGANNNNNNTGC 2 cut(s) 357, 391
BciT130I CCWGG 3 cut(s) 1062, 1372, 1447
BciVI GTATCC 1 cut(s) 656
BcnI CCSGG 2 cut(s) 207, 524
BcoDI GTCTC 4 cut(s) 187, 379, 960, 1407
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 5 cut(s) 105, 648, 935, 1038, 1068
BfmI CTRYAG 2 cut(s) 60, 588
BfuI GTATCC 1 cut(s) 656
BisI GCNGC 2 cut(s) 326, 1050
BlpI GCTNAGC 1 cut(s) 153
BlsI GCNGC 2 cut(s) 327, 1051
Bme1390I CCNGG 5 cut(s) 207, 524, 1062, 1372, 1447
Bme18I GGWCC 3 cut(s) 113, 203, 335
BmgBI CACGTC 2 cut(s) 707, 795
BmgT120I GGNCC 4 cut(s) 113, 203, 335, 1064
BmiI GGNNCC 4 cut(s) 115, 932, 1340, 1444
BmrFI CCNGG 5 cut(s) 207, 524, 1062, 1372, 1447
BmsI GCATC 2 cut(s) 231, 1315
BpmI CTGGAG 2 cut(s) 1322, 1468
Bpu1102I GCTNAGC 1 cut(s) 153
Bpu14I TTCGAA 1 cut(s) 778
BpuMI CCSGG 2 cut(s) 207, 524
BsaJI CCNNGG 6 cut(s) 206, 338, 522, 1060, 1061, 1371
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 1111, 1377
Bse1I ACTGG 3 cut(s) 1243, 1305, 1408
Bse21I CCTNAGG 1 cut(s) 1110
BseBI CCWGG 3 cut(s) 1062, 1372, 1447
BseDI CCNNGG 6 cut(s) 206, 338, 522, 1060, 1061, 1371
BseGI GGATG 3 cut(s) 1101, 1129, 1381
BseLI CCNNNNNNNGG 3 cut(s) 332, 1111, 1377
BseMII CTCAG 4 cut(s) 38, 158, 167, 1124
BseNI ACTGG 3 cut(s) 1243, 1305, 1408
BsePI GCGCGC 1 cut(s) 1052
BseRI GAGGAG 2 cut(s) 277, 1465
BseSI GKGCMC 1 cut(s) 933
BseXI GCAGC 1 cut(s) 1036
BseYI CCCAGC 2 cut(s) 410, 1204
BsgI GTGCAG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 1054
BshFI GGCC 2 cut(s) 834, 1066
BshNI GGYRCC 2 cut(s) 930, 1338
BsiHKAI GWGCWC 1 cut(s) 1037
BsiSI CCGG 2 cut(s) 206, 524
BslI CCNNNNNNNGG 3 cut(s) 332, 1111, 1377
BsmAI GTCTC 4 cut(s) 187, 379, 960, 1407
BsmBI CGTCTC 1 cut(s) 960
BsnI GGCC 2 cut(s) 834, 1066
Bsp119I TTCGAA 1 cut(s) 778
Bsp1286I GDGCHC 2 cut(s) 933, 1037
Bsp143I GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
Bsp1720I GCTNAGC 1 cut(s) 153
BspACI CCGC 4 cut(s) 214, 326, 903, 1106
BspANI GGCC 2 cut(s) 834, 1066
BspCNI CTCAG 4 cut(s) 39, 159, 166, 1123
BspFNI CGCG 1 cut(s) 1054
BspHI TCATGA 2 cut(s) 625, 1075
BspLI GGNNCC 4 cut(s) 115, 932, 1340, 1444
BspMAI CTGCAG 1 cut(s) 592
BspPI GGATC 4 cut(s) 403, 534, 592, 1249
BspQI GCTCTTC 1 cut(s) 1372
BspT104I TTCGAA 1 cut(s) 778
BspT107I GGYRCC 2 cut(s) 930, 1338
BsrI ACTGG 3 cut(s) 1243, 1305, 1408
BssECI CCNNGG 6 cut(s) 206, 338, 522, 1060, 1061, 1371
BssHII GCGCGC 1 cut(s) 1052
BssMI GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
BssSI CACGAG 1 cut(s) 603
BssT1I CCWWGG 1 cut(s) 338
Bst2BI CACGAG 1 cut(s) 603
Bst2UI CCWGG 3 cut(s) 1062, 1372, 1447
Bst4CI ACNGT 5 cut(s) 61, 226, 235, 502, 811
Bst6I CTCTTC 1 cut(s) 1372
BstBI TTCGAA 1 cut(s) 778
BstC8I GCNNGC 4 cut(s) 550, 901, 912, 1054
BstDEI CTNAG 4 cut(s) 47, 153, 167, 1110
BstF5I GGATG 3 cut(s) 1101, 1129, 1381
BstFNI CGCG 1 cut(s) 1054
BstHHI GCGC 2 cut(s) 1054, 1056
BstKTI GATC 8 cut(s) 173, 398, 529, 587, 658, 754, 1000, 1244
BstMAI GTCTC 4 cut(s) 187, 379, 960, 1407
BstMBI GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
BstMWI GCNNNNNNNGC 3 cut(s) 163, 957, 1049
BstNI CCWGG 3 cut(s) 1062, 1372, 1447
BstNSI RCATGY 2 cut(s) 556, 951
BstSCI CCNGG 5 cut(s) 205, 522, 1060, 1370, 1445
BstSFI CTRYAG 2 cut(s) 60, 588
BstSLI GKGCMC 1 cut(s) 933
BstUI CGCG 1 cut(s) 1054
BstV1I GCAGC 1 cut(s) 1036
BstX2I RGATCY 3 cut(s) 395, 584, 1241
BstYI RGATCY 3 cut(s) 395, 584, 1241
Bsu36I CCTNAGG 1 cut(s) 1110
BsuI GTATCC 1 cut(s) 656
BsuRI GGCC 2 cut(s) 834, 1066
BtgZI GCGATG 1 cut(s) 508
BtrI CACGTC 2 cut(s) 707, 795
BtsCI GGATG 3 cut(s) 1101, 1129, 1381
BtsI GCAGTG 1 cut(s) 1222
BtsIMutI CAGTG 6 cut(s) 44, 507, 807, 1222, 1236, 1401
Cac8I GCNNGC 4 cut(s) 550, 901, 912, 1054
CaiI CAGNNNCTG 1 cut(s) 506
CciI TCATGA 2 cut(s) 625, 1075
CfoI GCGC 2 cut(s) 1054, 1056
Cfr13I GGNCC 4 cut(s) 113, 203, 335, 1064
CpoI CGGWCCG 1 cut(s) 203
Csp6I GTAC 3 cut(s) 90, 1179, 1228
CspI CGGWCCG 1 cut(s) 203
CviAII CATG 8 cut(s) 143, 305, 479, 553, 626, 948, 1076, 1254
CviQI GTAC 3 cut(s) 90, 1179, 1228
DdeI CTNAG 4 cut(s) 47, 153, 167, 1110
DpnI GATC 8 cut(s) 172, 397, 528, 586, 657, 753, 999, 1243
DpnII GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 1372
EarI CTCTTC 1 cut(s) 1372
Eco130I CCWWGG 1 cut(s) 338
Eco32I GATATC 2 cut(s) 1021, 1073
Eco47I GGWCC 3 cut(s) 113, 203, 335
Eco81I CCTNAGG 1 cut(s) 1110
EcoO109I RGGNCCY 2 cut(s) 113, 1064
EcoRI GAATTC 1 cut(s) 1397
EcoRII CCWGG 3 cut(s) 1060, 1370, 1445
EcoRV GATATC 2 cut(s) 1021, 1073
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 953
ErhI CCWWGG 1 cut(s) 338
Esp3I CGTCTC 1 cut(s) 960
FaeI CATG 8 cut(s) 146, 308, 482, 556, 629, 951, 1079, 1257
FatI CATG 8 cut(s) 142, 304, 478, 552, 625, 947, 1075, 1253
FauI CCCGC 3 cut(s) 207, 896, 1113
FauNDI CATATG 1 cut(s) 370
FblI GTMKAC 1 cut(s) 1151
Fnu4HI GCNGC 2 cut(s) 326, 1050
FokI GGATG 3 cut(s) 1108, 1116, 1388
Fsp4HI GCNGC 2 cut(s) 326, 1050
FspBI CTAG 5 cut(s) 105, 648, 935, 1038, 1068
GlaI GCGC 2 cut(s) 1053, 1055
GluI GCNGC 2 cut(s) 326, 1050
GsaI CCCAGC 2 cut(s) 414, 1208
GsuI CTGGAG 2 cut(s) 1322, 1468
HaeIII GGCC 2 cut(s) 834, 1066
HapII CCGG 2 cut(s) 206, 524
HhaI GCGC 2 cut(s) 1054, 1056
Hin1II CATG 8 cut(s) 146, 308, 482, 556, 629, 951, 1079, 1257
Hin6I GCGC 2 cut(s) 1052, 1054
HinP1I GCGC 2 cut(s) 1052, 1054
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 4 cut(s) 65, 282, 599, 734
HpaII CCGG 2 cut(s) 206, 524
HphI GGTGA 5 cut(s) 247, 549, 1288, 1423, 1455
Hpy166II GTNNAC 3 cut(s) 805, 1152, 1181
Hpy188I TCNGA 6 cut(s) 70, 203, 799, 1002, 1138, 1224
Hpy188III TCNNGA 5 cut(s) 270, 626, 755, 1024, 1076
Hpy8I GTNNAC 3 cut(s) 805, 1152, 1181
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 8 cut(s) 104, 242, 267, 463, 679, 785, 932, 1431
HpyCH4III ACNGT 5 cut(s) 61, 226, 235, 502, 811
HpyCH4IV ACGT 4 cut(s) 706, 794, 886, 973
HpyCH4V TGCA 4 cut(s) 466, 548, 590, 951
HpyF10VI GCNNNNNNNGC 3 cut(s) 163, 957, 1049
HpyF3I CTNAG 4 cut(s) 47, 153, 167, 1110
HpySE526I ACGT 4 cut(s) 706, 794, 886, 973
Hsp92II CATG 8 cut(s) 146, 308, 482, 556, 629, 951, 1079, 1257
HspAI GCGC 2 cut(s) 1052, 1054
Kzo9I GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
LguI GCTCTTC 1 cut(s) 1372
LmnI GCTCC 3 cut(s) 1196, 1303, 1442
Lsp1109I GCAGC 1 cut(s) 1036
LweI GCATC 2 cut(s) 231, 1315
MaeI CTAG 5 cut(s) 105, 648, 935, 1038, 1068
MaeII ACGT 4 cut(s) 706, 794, 886, 973
MaeIII GTNAC 4 cut(s) 7, 502, 555, 974
MalI GATC 8 cut(s) 172, 397, 528, 586, 657, 753, 999, 1243
MboI GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
MboII GAAGA 2 cut(s) 250, 1359
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 3 cut(s) 395, 584, 1241
MhlI GDGCHC 2 cut(s) 933, 1037
MlsI TGGCCA 1 cut(s) 834
MluCI AATT 8 cut(s) 15, 262, 621, 689, 711, 780, 1249, 1397
MluNI TGGCCA 1 cut(s) 834
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 181, 1273
MnlI CCTC 8 cut(s) 204, 250, 298, 622, 653, 1119, 1375, 1443
Mox20I TGGCCA 1 cut(s) 834
Mph1103I ATGCAT 1 cut(s) 953
MscI TGGCCA 1 cut(s) 834
MseI TTAA 2 cut(s) 432, 576
Msp20I TGGCCA 1 cut(s) 834
MspA1I CMGCKG 3 cut(s) 328, 903, 1319
MspI CCGG 2 cut(s) 206, 524
MspR9I CCNGG 5 cut(s) 207, 524, 1062, 1372, 1447
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 3 cut(s) 1062, 1372, 1447
MvnI CGCG 1 cut(s) 1054
MwoI GCNNNNNNNGC 3 cut(s) 163, 957, 1049
NciI CCSGG 2 cut(s) 207, 524
NdeI CATATG 1 cut(s) 370
NdeII GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
NlaIII CATG 8 cut(s) 146, 308, 482, 556, 629, 951, 1079, 1257
NlaIV GGNNCC 4 cut(s) 115, 932, 1340, 1444
NmeAIII GCCGAG 1 cut(s) 389
NmuCI GTSAC 2 cut(s) 502, 555
NsiI ATGCAT 1 cut(s) 953
NspI RCATGY 2 cut(s) 556, 951
NspV TTCGAA 1 cut(s) 778
PagI TCATGA 2 cut(s) 625, 1075
PasI CCCWGGG 1 cut(s) 1061
PauI GCGCGC 1 cut(s) 1052
PciSI GCTCTTC 1 cut(s) 1372
PfeI GAWTC 3 cut(s) 282, 599, 734
PkrI GCNGC 2 cut(s) 327, 1051
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 113
PsiI TTATAA 1 cut(s) 1158
Psp5II RGGWCCY 1 cut(s) 113
Psp6I CCWGG 3 cut(s) 1060, 1370, 1445
PspFI CCCAGC 2 cut(s) 410, 1204
PspGI CCWGG 3 cut(s) 1060, 1370, 1445
PspN4I GGNNCC 4 cut(s) 115, 932, 1340, 1444
PspPI GGNCC 4 cut(s) 113, 203, 335, 1064
PspPPI RGGWCCY 1 cut(s) 113
PstI CTGCAG 1 cut(s) 592
PstNI CAGNNNCTG 1 cut(s) 506
PsuI RGATCY 3 cut(s) 395, 584, 1241
PteI GCGCGC 1 cut(s) 1052
PvuII CAGCTG 1 cut(s) 1319
RsaI GTAC 3 cut(s) 91, 1180, 1229
RsaNI GTAC 3 cut(s) 90, 1179, 1228
Rsr2I CGGWCCG 1 cut(s) 203
RsrII CGGWCCG 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 1372
SaqAI TTAA 2 cut(s) 432, 576
SatI GCNGC 2 cut(s) 326, 1050
Sau3AI GATC 8 cut(s) 170, 395, 526, 584, 655, 751, 997, 1241
Sau96I GGNCC 4 cut(s) 113, 203, 335, 1064
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 5 cut(s) 207, 524, 1062, 1372, 1447
SduI GDGCHC 2 cut(s) 933, 1037
SfaNI GCATC 2 cut(s) 231, 1315
SfcI CTRYAG 2 cut(s) 60, 588
SfuI TTCGAA 1 cut(s) 778
SinI GGWCC 3 cut(s) 113, 203, 335
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 8 cut(s) 15, 262, 621, 689, 711, 780, 1249, 1397
SsiI CCGC 4 cut(s) 214, 326, 903, 1106
SspI AATATT 1 cut(s) 1460
SspMI CTAG 5 cut(s) 105, 648, 935, 1038, 1068
StyD4I CCNGG 5 cut(s) 205, 522, 1060, 1370, 1445
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 5 cut(s) 61, 226, 235, 502, 811
TaiI ACGT 4 cut(s) 709, 797, 889, 976
TaqI TCGA 4 cut(s) 27, 230, 658, 778
TaqII GACCGA 1 cut(s) 1159
TasI AATT 8 cut(s) 15, 262, 621, 689, 711, 780, 1249, 1397
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 1 cut(s) 328
TfiI GAWTC 3 cut(s) 282, 599, 734
Tru1I TTAA 2 cut(s) 432, 576
Tru9I TTAA 2 cut(s) 432, 576
TscAI CASTG 6 cut(s) 51, 507, 814, 1222, 1243, 1408
TseFI GTSAC 2 cut(s) 502, 555
TseI GCWGC 1 cut(s) 1049
Tsp45I GTSAC 2 cut(s) 502, 555
TspDTI ATGAA 9 cut(s) 159, 467, 557, 614, 840, 970, 1092, 1139, 1242
TspGWI ACGGA 3 cut(s) 114, 632, 984
TspRI CASTG 6 cut(s) 51, 507, 814, 1222, 1243, 1408
VpaK11BI GGWCC 3 cut(s) 113, 203, 335
XapI RAATTY 4 cut(s) 621, 689, 780, 1397
XceI RCATGY 2 cut(s) 556, 951
XmiI GTMKAC 1 cut(s) 1151
XspI CTAG 5 cut(s) 105, 648, 935, 1038, 1068
Zsp2I ATGCAT 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.