Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001751

Physical Location & Seq

Genomic Coordinates Reverse (-)

41315447 .. 41319659

Gene Structure 4213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1294100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGGATATTCACTCAGTAGATTGATACCAGAATCTGACCATTTTGATCATGGAAATGCTGCCCTCCATCCCAACAAACCATTGCATAATATAGAACATGAAGTAGCCCAGCTCACAAAGTTGAGATCAAATCCCAATGAGCGGCTTACCCGAATTGGACCTAGAAGGCAGGACTTACCTGTTTCCACGATCAGGATGCTTGCTGGTCGAGAGGGTAATTATTCGGGAAGAGGGGGAAGGTTCTCATCCGCAGATCGTTGCCATGTTCTAAGTAGATATTTGCCGGTCAATGGTCCTTGGCTTGTCGACAAAATGTCTAGCCGAGCTTATGTATCTCAGTTTTCGGCAGATGGATCTCTTTTCATTGCTGGGTTTCAGGATAGCCACATTAGAATATACAATGTGGATAGAGGGTGGAAAGTCCAGAAGAACATCCATGCCAAAAGTATGCAATGGTCTGTCACTGATACGTCTCTTTCCCCGGATCAACGCCATCTTGTTTATGCGAGTATGTCCCCTATTGTTCATATGGTAGATGTGGGATCTTCTGAAACGGAGTCTCCTGCAAATATAACGGAAATCCATGAGGGTTTGGATTTTTCCTCTGGTGATGATGATGGAGGGTACTCATTTGGTATTTTCTCCATAAAATTCTCAACAGATGGGCATGAACTTGTTGCTGGAAGTAGTGATGATTCCATATATGTTTATGATCTTGAAGCCAATAAGCTCTCCCTTCGGATTCCGGCACACTCTTCTGATGTGAACACTGTATGCTTTGCCGATGAAGGTGACCATCTTATTTATTCTGGGAGTGACGATACTCTCTGTAAGGTGTGGGACAGACGTTGCCTTAAAACCACAGGGAAGCCAGCAGGAGTCTTGACGGGACACCTAGAAGGTATTGCATTCATTGACAGCAAGGGAGATGGTCGTTATTTCATTTCGAACGGTAAAGATCAGACCATTAAACTTTGGGATATCAGGAAAATTTCTTCTAGTCCCACTCGCACTCCAGGGATTAGGATTTCTGATTGGGATTACGGATGGATGGACTACCCATCTCAGGCAAAAGATATGAAACATCCCTCTGATCAATCAGTAGCCACGTATAGAGGACATTCAGTCTTGCGCACGCTTATTCGCTGCTACTTTTCTCCGGCATATAGCACCGGTCAGAAGTACATCTACACGGGGTCTCATAACTCTTACGTTTATATATATGATTTGGTGACTGGAGCCCAAGTTGCAGCGCTCAAGCATCATAAAGAACCTGTAAGAGACTGTAGCTGGCACCCCTATTACCCGATGCTCATAAGCTCTTCTTGGGACGGGGGAGTTGTTAGATGGGAATTCCCCGGGAGTGGAGAAGCGCCGGCGCCTTACCCCCGAAAGAGACTCTGGAGGAGACAGTTTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

474

Amino Acids

53.2

Weight (kDa)

7.34

Isoelectric Point (pI)

46.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Prp19	PF24814	113 - 288	3.7e-15	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	120 - 331	6.8e-10	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	124 - 277	4.5e-08	WDR36/Utp21 first beta-propeller
WD40_Gbeta	PF25391	208 - 284	5.2e-08	G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd	PF25172	208 - 327	1e-11	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	209 - 283	8.9e-14	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	210 - 281	2.6e-06	MABP1/WDR62 second WD40 domain
WD40_Prp19	PF24814	212 - 330	5.8e-14	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	213 - 333	7.7e-14	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	213 - 282	1.7e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	213 - 328	8.6e-18	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	214 - 336	1.3e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	214 - 331	1e-17	THOC3 beta-propeller domain
WDR55	PF24796	215 - 330	4.3e-14	WDR55
WD40	PF00400	246 - 281	1.2e-06	WD domain, G-beta repeat
EIF3I	PF24805	250 - 444	9e-12	EIF3I
Beta-prop_WDR5	PF25175	364 - 447	3.3e-09	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	388 - 451	4.8e-08	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	392 - 453	1.8e-06	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001751)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03020 AT4G03020 AT4G03020
fragaria_vesca	FvH4_2g24350 FvH4_2g24350 FvH4_2g24350 FvH4_4g16870 FvH4_4g16870
malus_domestica	MD03G1273100.v1.1 MD11G1294100.v1.1 MD13G1176000.v1.1 MD16G1177700.v1.1
prunus_persica	Prupe.1G145000_v2.0.a1 Prupe.1G145000_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1
pyrus_communis	pycom03g21760 pycom11g26020 pycom13g15170
rosa_chinensis	RchiOBHm_Chr4g0420461 RchiOBHm_Chr6g0292221
rosa_laevigata	RLG00000007761 RLG00000012052
rosa_multiflora	Rmu_co8469755.1_g000001 Rmu_sc0002248.1_g000028 Rmu_sc0002993.1_g000006 Rmu_sc0017106.1_g000005
rosa_roxburghii	Rroxscaffold_5G00363080 Rroxscaffold_7G00175340
rosa_rugosa	Rorug04G0163900 Rorug06G0224500 Rorug06G0224500
rosa_samantha	Rh4CG239900 Rh6AG337600 Rh6BG344900 Rh6CG352000
rosa_wichuraiana	Rw4G019470 Rw6G029400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1133
AccB1I	GGYRCC	2 cut(s)	1293, 1378
AccBSI	CCGCTC	1 cut(s)	142
AccI	GTMKAC	1 cut(s)	306
AciI	CCGC	2 cut(s)	142, 249
AclWI	GGATC	3 cut(s)	361, 492, 550
AcsI	RAATTY	3 cut(s)	650, 990, 1352
AcyI	GRCGYC	1 cut(s)	1379
AfaI	GTAC	2 cut(s)	626, 1184
AfeI	AGCGCT	1 cut(s)	1254
AfiI	CCNNNNNNNGG	5 cut(s)	141, 192, 290, 1066, 1364
AgeI	ACCGGT	1 cut(s)	1172
AgsI	TTSAA	1 cut(s)	719
AhdI	GACNNNNNGTC	2 cut(s)	313, 1124
AjnI	CCWGG	1 cut(s)	1015
AluBI	AGCT	5 cut(s)	112, 326, 730, 1290, 1320
AluI	AGCT	5 cut(s)	112, 326, 730, 1290, 1320
Alw26I	GTCTC	6 cut(s)	477, 564, 1203, 1275, 1390, 1402
AlwI	GGATC	3 cut(s)	361, 492, 550
AlwNI	CAGNNNCTG	1 cut(s)	35
Ama87I	CYCGRG	1 cut(s)	1358
Aor51HI	AGCGCT	1 cut(s)	1254
ApeKI	GCWGC	3 cut(s)	59, 1146, 1250
ApoI	RAATTY	3 cut(s)	650, 990, 1352
AsiGI	ACCGGT	1 cut(s)	1172
AspLEI	GCGC	4 cut(s)	1134, 1255, 1375, 1381
AspS9I	GGNCC	2 cut(s)	158, 293
AsuC2I	CCSGG	3 cut(s)	482, 1359, 1360
AsuHPI	GGTGA	3 cut(s)	620, 803, 1243
AsuII	TTCGAA	1 cut(s)	947
AvaI	CYCGRG	1 cut(s)	1358
AvaII	GGWCC	2 cut(s)	158, 293
BanI	GGYRCC	2 cut(s)	1293, 1378
BanII	GRGCYC	1 cut(s)	1243
BarI	GAAGNNNNNNTAC	2 cut(s)	1172, 1204
BbvI	GCAGC	3 cut(s)	46, 1133, 1262
BcgI	CGANNNNNNTGC	2 cut(s)	178, 212
BciT130I	CCWGG	1 cut(s)	1017
BclI	TGATCA	2 cut(s)	46, 1093
BcnI	CCSGG	3 cut(s)	482, 1359, 1360
BcoDI	GTCTC	6 cut(s)	477, 564, 1203, 1275, 1390, 1402
BfaI	CTAG	4 cut(s)	162, 318, 896, 999
BfmI	CTRYAG	1 cut(s)	1285
BfoI	RGCGCY	3 cut(s)	1256, 1376, 1382
BisI	GCNGC	4 cut(s)	60, 143, 1147, 1251
BlsI	GCNGC	4 cut(s)	61, 144, 1148, 1252
Bme1390I	CCNGG	4 cut(s)	482, 1017, 1359, 1360
Bme18I	GGWCC	2 cut(s)	158, 293
BmeRI	GACNNNNNGTC	2 cut(s)	313, 1124
BmeT110I	CYCGRG	1 cut(s)	1358
BmgT120I	GGNCC	2 cut(s)	158, 293
BmiI	GGNNCC	3 cut(s)	1240, 1295, 1380
BmrFI	CCNGG	4 cut(s)	482, 1017, 1359, 1360
BmsI	GCATC	3 cut(s)	186, 1270, 1299
BpmI	CTGGAG	2 cut(s)	999, 1257
Bpu14I	TTCGAA	1 cut(s)	947
BpuEI	CTTGAG	1 cut(s)	1241
BpuMI	CCSGG	3 cut(s)	482, 1359, 1360
BsaAI	YACGTR	1 cut(s)	1110
BsaHI	GRCGYC	1 cut(s)	1379
BsaI	GGTCTC	1 cut(s)	1203
BsaJI	CCNNGG	5 cut(s)	296, 480, 1016, 1357, 1358
BsaWI	WCCGGW	1 cut(s)	1172
BsaXI	ACNNNNNCTCC	6 cut(s)	544, 574, 997, 1027, 1230, 1260
Bsc4I	CCNNNNNNNGG	5 cut(s)	141, 192, 290, 1066, 1364
Bse118I	RCCGGY	3 cut(s)	283, 1172, 1375
Bse1I	ACTGG	1 cut(s)	1240
Bse3DI	GCAATG	3 cut(s)	80, 363, 458
BseBI	CCWGG	1 cut(s)	1017
BseDI	CCNNGG	5 cut(s)	296, 480, 1016, 1357, 1358
BseGI	GGATG	7 cut(s)	67, 201, 245, 432, 1052, 1056, 1084
BseLI	CCNNNNNNNGG	5 cut(s)	141, 192, 290, 1066, 1364
BseMI	GCAATG	3 cut(s)	80, 363, 458
BseMII	CTCAG	3 cut(s)	28, 350, 1079
BseNI	ACTGG	1 cut(s)	1240
BseRI	GAGGAG	1 cut(s)	1420
BseXI	GCAGC	3 cut(s)	46, 1133, 1262
BseYI	CCCAGC	2 cut(s)	108, 368
BshNI	GGYRCC	2 cut(s)	1293, 1378
BshTI	ACCGGT	1 cut(s)	1172
BsiHKCI	CYCGRG	1 cut(s)	1358
BsiSI	CCGG	7 cut(s)	284, 482, 746, 1160, 1173, 1359, 1376
BslFI	GGGAC	5 cut(s)	499, 854, 903, 987, 1343
BslI	CCNNNNNNNGG	5 cut(s)	141, 192, 290, 1066, 1364
BsmAI	GTCTC	6 cut(s)	477, 564, 1203, 1275, 1390, 1402
BsmBI	CGTCTC	1 cut(s)	477
BsmFI	GGGAC	5 cut(s)	499, 854, 903, 987, 1343
BsmI	GAATGC	1 cut(s)	908
Bso31I	GGTCTC	1 cut(s)	1203
BsoBI	CYCGRG	1 cut(s)	1358
Bsp119I	TTCGAA	1 cut(s)	947
Bsp1286I	GDGCHC	1 cut(s)	1243
BspACI	CCGC	2 cut(s)	142, 249
BspCNI	CTCAG	3 cut(s)	27, 349, 1078
BspLI	GGNNCC	3 cut(s)	1240, 1295, 1380
BspPI	GGATC	3 cut(s)	361, 492, 550
BspQI	GCTCTTC	1 cut(s)	1327
BspT104I	TTCGAA	1 cut(s)	947
BspT107I	GGYRCC	2 cut(s)	1293, 1378
BspTNI	GGTCTC	1 cut(s)	1203
BsrBI	CCGCTC	1 cut(s)	142
BsrDI	GCAATG	3 cut(s)	80, 363, 458
BsrFI	RCCGGY	3 cut(s)	283, 1172, 1375
BsrI	ACTGG	1 cut(s)	1240
BssAI	RCCGGY	3 cut(s)	283, 1172, 1375
BssECI	CCNNGG	5 cut(s)	296, 480, 1016, 1357, 1358
BssNI	GRCGYC	1 cut(s)	1379
BssT1I	CCWWGG	1 cut(s)	296
Bst2UI	CCWGG	1 cut(s)	1017
Bst4CI	ACNGT	4 cut(s)	772, 953, 1286, 1413
Bst6I	CTCTTC	3 cut(s)	223, 760, 1327
BstACI	GRCGYC	1 cut(s)	1379
BstBAI	YACGTR	1 cut(s)	1110
BstBI	TTCGAA	1 cut(s)	947
BstC8I	GCNNGC	5 cut(s)	201, 873, 1136, 1292, 1377
BstDEI	CTNAG	4 cut(s)	14, 269, 336, 1065
BstEII	GGTNACC	1 cut(s)	791
BstF5I	GGATG	7 cut(s)	67, 201, 245, 432, 1052, 1056, 1084
BstH2I	RGCGCY	3 cut(s)	1256, 1376, 1382
BstHHI	GCGC	4 cut(s)	1134, 1255, 1375, 1381
BstMAI	GTCTC	6 cut(s)	477, 564, 1203, 1275, 1390, 1402
BstMWI	GCNNNNNNNGC	1 cut(s)	1247
BstNI	CCWGG	1 cut(s)	1017
BstPI	GGTNACC	1 cut(s)	791
BstSCI	CCNGG	4 cut(s)	480, 1015, 1357, 1358
BstSFI	CTRYAG	1 cut(s)	1285
BstV1I	GCAGC	3 cut(s)	46, 1133, 1262
BstX2I	RGATCY	2 cut(s)	353, 542
BstYI	RGATCY	2 cut(s)	353, 542
BtsCI	GGATG	7 cut(s)	67, 201, 245, 432, 1052, 1056, 1084
BtsIMutI	CAGTG	2 cut(s)	462, 768
Cac8I	GCNNGC	5 cut(s)	201, 873, 1136, 1292, 1377
CaiI	CAGNNNCTG	1 cut(s)	35
CfoI	GCGC	4 cut(s)	1134, 1255, 1375, 1381
Cfr10I	RCCGGY	3 cut(s)	283, 1172, 1375
Cfr13I	GGNCC	2 cut(s)	158, 293
Cfr9I	CCCGGG	1 cut(s)	1358
Csp6I	GTAC	2 cut(s)	625, 1183
CspAI	ACCGGT	1 cut(s)	1172
CviAII	CATG	6 cut(s)	50, 98, 263, 437, 584, 668
CviQI	GTAC	2 cut(s)	625, 1183
DdeI	CTNAG	4 cut(s)	14, 269, 336, 1065
DinI	GGCGCC	1 cut(s)	1380
DriI	GACNNNNNGTC	2 cut(s)	313, 1124
Eam1104I	CTCTTC	3 cut(s)	223, 760, 1327
Eam1105I	GACNNNNNGTC	2 cut(s)	313, 1124
EarI	CTCTTC	3 cut(s)	223, 760, 1327
Eco130I	CCWWGG	1 cut(s)	296
Eco24I	GRGCYC	1 cut(s)	1243
Eco31I	GGTCTC	1 cut(s)	1203
Eco32I	GATATC	1 cut(s)	982
Eco47I	GGWCC	2 cut(s)	158, 293
Eco47III	AGCGCT	1 cut(s)	1254
Eco88I	CYCGRG	1 cut(s)	1358
Eco91I	GGTNACC	1 cut(s)	791
EcoO65I	GGTNACC	1 cut(s)	791
EcoRI	GAATTC	1 cut(s)	1352
EcoRII	CCWGG	1 cut(s)	1015
EcoRV	GATATC	1 cut(s)	982
EcoT14I	CCWWGG	1 cut(s)	296
EcoT38I	GRGCYC	1 cut(s)	1243
EgeI	GGCGCC	1 cut(s)	1380
EheI	GGCGCC	1 cut(s)	1380
ErhI	CCWWGG	1 cut(s)	296
Esp3I	CGTCTC	1 cut(s)	477
FaeI	CATG	6 cut(s)	53, 101, 266, 440, 587, 671
FalI	AAGNNNNNCTT	2 cut(s)	1309, 1341
FaqI	GGGAC	5 cut(s)	499, 854, 903, 987, 1343
FatI	CATG	6 cut(s)	49, 97, 262, 436, 583, 667
FauNDI	CATATG	1 cut(s)	528
FbaI	TGATCA	2 cut(s)	46, 1093
FblI	GTMKAC	1 cut(s)	306
Fnu4HI	GCNGC	4 cut(s)	60, 143, 1147, 1251
FokI	GGATG	7 cut(s)	54, 208, 232, 419, 1059, 1063, 1071
FriOI	GRGCYC	1 cut(s)	1243
Fsp4HI	GCNGC	4 cut(s)	60, 143, 1147, 1251
FspBI	CTAG	4 cut(s)	162, 318, 896, 999
FspI	TGCGCA	1 cut(s)	1133
GlaI	GCGC	4 cut(s)	1133, 1254, 1374, 1380
GluI	GCNGC	4 cut(s)	60, 143, 1147, 1251
GsaI	CCCAGC	2 cut(s)	112, 372
GsuI	CTGGAG	2 cut(s)	999, 1257
HaeII	RGCGCY	3 cut(s)	1256, 1376, 1382
HapII	CCGG	7 cut(s)	284, 482, 746, 1160, 1173, 1359, 1376
HhaI	GCGC	4 cut(s)	1134, 1255, 1375, 1381
Hin1I	GRCGYC	1 cut(s)	1379
Hin1II	CATG	6 cut(s)	53, 101, 266, 440, 587, 671
Hin6I	GCGC	4 cut(s)	1132, 1253, 1373, 1379
HinP1I	GCGC	4 cut(s)	1132, 1253, 1373, 1379
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HinfI	GANTC	6 cut(s)	32, 557, 695, 742, 879, 1398
HpaII	CCGG	7 cut(s)	284, 482, 746, 1160, 1173, 1359, 1376
HphI	GGTGA	3 cut(s)	620, 803, 1243
Hpy166II	GTNNAC	2 cut(s)	307, 766
Hpy188I	TCNGA	8 cut(s)	37, 550, 741, 760, 963, 1033, 1093, 1179
Hpy188III	TCNNGA	9 cut(s)	193, 209, 225, 377, 424, 716, 883, 985, 1402
Hpy8I	GTNNAC	2 cut(s)	307, 766
HpyAV	CCTTC	5 cut(s)	159, 231, 746, 782, 893
HpyCH4III	ACNGT	4 cut(s)	772, 953, 1286, 1413
HpyCH4IV	ACGT	4 cut(s)	470, 847, 1109, 1212
HpyCH4V	TGCA	5 cut(s)	85, 451, 566, 908, 1250
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1247
HpyF3I	CTNAG	4 cut(s)	14, 269, 336, 1065
HpySE526I	ACGT	4 cut(s)	470, 847, 1109, 1212
Hsp92I	GRCGYC	1 cut(s)	1379
Hsp92II	CATG	6 cut(s)	53, 101, 266, 440, 587, 671
HspAI	GCGC	4 cut(s)	1132, 1253, 1373, 1379
KasI	GGCGCC	1 cut(s)	1378
KroI	GCCGGC	1 cut(s)	1375
KroNI	GCCGGC	1 cut(s)	1377
Ksp22I	TGATCA	2 cut(s)	46, 1093
LguI	GCTCTTC	1 cut(s)	1327
LmnI	GCTCC	1 cut(s)	1238
Lsp1109I	GCAGC	3 cut(s)	46, 1133, 1262
LweI	GCATC	3 cut(s)	186, 1270, 1299
MaeI	CTAG	4 cut(s)	162, 318, 896, 999
MaeII	ACGT	4 cut(s)	470, 847, 1109, 1212
MaeIII	GTNAC	4 cut(s)	460, 791, 815, 1231
MbiI	CCGCTC	1 cut(s)	142
MboII	GAAGA	6 cut(s)	240, 439, 537, 747, 987, 1314
MflI	RGATCY	2 cut(s)	353, 542
MhlI	GDGCHC	1 cut(s)	1243
MluCI	AATT	5 cut(s)	153, 217, 650, 990, 1352
Mly113I	GGCGCC	1 cut(s)	1379
MlyI	GAGTC	3 cut(s)	566, 888, 1392
MreI	CGCCGGCG	1 cut(s)	1375
MroNI	GCCGGC	1 cut(s)	1375
MseI	TTAA	2 cut(s)	855, 969
MslI	CAYNNNNRTG	1 cut(s)	54
MspI	CCGG	7 cut(s)	284, 482, 746, 1160, 1173, 1359, 1376
MspR9I	CCNGG	4 cut(s)	482, 1017, 1359, 1360
Mva1269I	GAATGC	1 cut(s)	908
MvaI	CCWGG	1 cut(s)	1017
MwoI	GCNNNNNNNGC	1 cut(s)	1247
NaeI	GCCGGC	1 cut(s)	1377
NarI	GGCGCC	1 cut(s)	1379
NciI	CCSGG	3 cut(s)	482, 1359, 1360
NdeI	CATATG	1 cut(s)	528
NgoMIV	GCCGGC	1 cut(s)	1375
NlaIII	CATG	6 cut(s)	53, 101, 266, 440, 587, 671
NlaIV	GGNNCC	3 cut(s)	1240, 1295, 1380
NmeAIII	GCCGAG	1 cut(s)	347
NmuCI	GTSAC	4 cut(s)	460, 791, 815, 1231
NsbI	TGCGCA	1 cut(s)	1133
NspV	TTCGAA	1 cut(s)	947
PciSI	GCTCTTC	1 cut(s)	1327
PctI	GAATGC	1 cut(s)	908
PdiI	GCCGGC	1 cut(s)	1377
PfeI	GAWTC	3 cut(s)	32, 695, 742
PinAI	ACCGGT	1 cut(s)	1172
PkrI	GCNGC	4 cut(s)	61, 144, 1148, 1252
PleI	GAGTC	3 cut(s)	565, 887, 1392
PluTI	GGCGCC	1 cut(s)	1382
PpsI	GAGTC	3 cut(s)	565, 887, 1392
Ppu21I	YACGTR	1 cut(s)	1110
Psp6I	CCWGG	1 cut(s)	1015
PspEI	GGTNACC	1 cut(s)	791
PspFI	CCCAGC	2 cut(s)	108, 368
PspGI	CCWGG	1 cut(s)	1015
PspN4I	GGNNCC	3 cut(s)	1240, 1295, 1380
PspPI	GGNCC	2 cut(s)	158, 293
PstNI	CAGNNNCTG	1 cut(s)	35
PsuI	RGATCY	2 cut(s)	353, 542
RsaI	GTAC	2 cut(s)	626, 1184
RsaNI	GTAC	2 cut(s)	625, 1183
RseI	CAYNNNNRTG	1 cut(s)	54
SalI	GTCGAC	1 cut(s)	305
SapI	GCTCTTC	1 cut(s)	1327
SaqAI	TTAA	2 cut(s)	855, 969
SatI	GCNGC	4 cut(s)	60, 143, 1147, 1251
Sau96I	GGNCC	2 cut(s)	158, 293
SchI	GAGTC	3 cut(s)	566, 888, 1392
ScrFI	CCNGG	4 cut(s)	482, 1017, 1359, 1360
SduI	GDGCHC	1 cut(s)	1243
SfaNI	GCATC	3 cut(s)	186, 1270, 1299
SfcI	CTRYAG	1 cut(s)	1285
SfoI	GGCGCC	1 cut(s)	1380
SfuI	TTCGAA	1 cut(s)	947
SgrAI	CRCCGGYG	1 cut(s)	1375
SinI	GGWCC	2 cut(s)	158, 293
SmaI	CCCGGG	1 cut(s)	1360
SmiMI	CAYNNNNRTG	1 cut(s)	54
SmlI	CTYRAG	1 cut(s)	1256
SmoI	CTYRAG	1 cut(s)	1256
Sse9I	AATT	5 cut(s)	153, 217, 650, 990, 1352
SsiI	CCGC	2 cut(s)	142, 249
SspDI	GGCGCC	1 cut(s)	1378
SspMI	CTAG	4 cut(s)	162, 318, 896, 999
StyD4I	CCNGG	4 cut(s)	480, 1015, 1357, 1358
StyI	CCWWGG	1 cut(s)	296
TaaI	ACNGT	4 cut(s)	772, 953, 1286, 1413
TaiI	ACGT	4 cut(s)	473, 850, 1112, 1215
TaqI	TCGA	3 cut(s)	208, 306, 947
TasI	AATT	5 cut(s)	153, 217, 650, 990, 1352
TatI	WGTACW	1 cut(s)	1182
TauI	GCSGC	1 cut(s)	145
TfiI	GAWTC	3 cut(s)	32, 695, 742
Tru1I	TTAA	2 cut(s)	855, 969
Tru9I	TTAA	2 cut(s)	855, 969
TscAI	CASTG	2 cut(s)	469, 775
TseFI	GTSAC	4 cut(s)	460, 791, 815, 1231
TseI	GCWGC	3 cut(s)	59, 1146, 1250
Tsp45I	GTSAC	4 cut(s)	460, 791, 815, 1231
TspDTI	ATGAA	8 cut(s)	114, 352, 515, 684, 801, 901, 931, 1094
TspGWI	ACGGA	3 cut(s)	569, 590, 1059
TspMI	CCCGGG	1 cut(s)	1358
TspRI	CASTG	2 cut(s)	469, 775
VpaK11BI	GGWCC	2 cut(s)	158, 293
XapI	RAATTY	3 cut(s)	650, 990, 1352
XcmI	CCANNNNNNNNNTGG	1 cut(s)	47
XmaI	CCCGGG	1 cut(s)	1358
XmiI	GTMKAC	1 cut(s)	306
XspI	CTAG	4 cut(s)	162, 318, 896, 999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.