FvH4_4g16870

LEC14B homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001751
Physical Location & Seq
Reverse (-)
20761660 .. 20767924
6265 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16870.t2

Sequence Viewer

Length: 1215 bp
ATGTTGATGGGCCGTGAGAGTAATTATTCGGGACGAGGGAGGTTTTCATCAGCGGATGCTTGTCATGTTTTAAGTCGGTATCTGCCCGTTAATGGTCCCTGGGGGGTGGACCAGTCAAATAGCCCTGCATATGTTTCTCAGTTTTCGAACAATGGCTTGTTTTTTGTTGCTGGGTTTCAGGGAGGCCATATTAGAATATATGATGTTGAAAAGGGATGGACAATTCAGAAGGACATTCGAACCAAAAGCTTGAGATGGACAATTACTGATACATCTCTATCACCAGATCAACGAAATCTTGCATATGCTAGCATGTCACCTATTGTCCATATTGTCAATGCTGGATCTTCTACGACAGAGTCAGTTGCAAACATTACGGAAGTACATGAAGGTCTCGATTTTTCTGTTGATTATGATGATGAAGAATTTGGAATATTCTCTGTTAAATTTTCAACTGATGGAAGAGAGCTTGTGGCTGGAAGTAGTGATGCTTCAATACATGTTTATGATCTCCAAGCAAACAAAGTTAGCCTCAACATACCAGCACACACGTCAGATGTAAACACAGTATGCTTTGCAGATGAGACTGGCCATCTTATATTTTCCGGCAGTGATGATAACCTCTGTAAGGTGTGGGATAGACGCTGCTTTGTCACGAAAGGGCAAGCAGCCGGGGTTCTGATGGGACATCAGGAAGGCATAACGTTTATTGATAGTCGGGGAGATGGGCGTTACTTAATATCAAATGGGAAGGATCAGACTACCAAACTTTGGGATATACGGAAGATGTCCTCTAGAAATTTGTATCCAAGGATTACAGATCATGACTGGGATTACAGATGGATGGAGTACCCAGGTCATTTAAGAACTTCTAAACATCCCTTTGATCAGTCCTTGGCTACATTTAGAGACCATTCAGTCCTGCGCACTTTAATTCGCTGCTACTTTTCTCCAGCATATAGCACTGGACAAAAGTACATTTACACTGGATCTAGTGATCATGCAGTCTATATTTATGATCTGGTGACTGGTGCTCAAGTTGCAAAACTCGTCCATCATGATGAACCGGTAAGAGATTGTAGTTGGCATCCTACTTATCCCATGCTGGTCAGTTCATCCTGGGATGGAAGTATCGCGAGATGGGAGTTTCCTGGAGATGATCAAGTTCCTACAGTGGTAAAGCCAAGAGTAAGGCGGAGGAGGGCTTTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

405

Amino Acids

45.65

Weight (kDa)

6.34

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR90_POC16_2nd PF23393 110 - 335 4.6e-06 WDR90/POC16, second beta-propeller
Beta-prop_RIG_2nd PF23775 115 - 249 9.4e-06 RIG second beta-propeller
Beta-prop_WDR5 PF25175 116 - 331 1.2e-21 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 128 - 282 1.3e-08 WDR36/Utp21 first beta-propeller
WDR55 PF24796 157 - 333 1.1e-15 WDR55
WD40_MABP1-WDR62_2nd PF24782 167 - 283 2.2e-08 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 186 - 285 2.4e-11 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 206 - 329 2.4e-13 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 206 - 348 3.7e-17 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 207 - 285 3.4e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 212 - 333 6.9e-20 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 213 - 333 1.3e-15 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 215 - 348 6.8e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 215 - 335 1.6e-18 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 216 - 283 2.6e-07 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 225 - 335 1.9e-09 TEP-1 second beta-propeller
WD40 PF00400 249 - 283 2.8e-06 WD domain, G-beta repeat
EIF3I PF24805 252 - 332 6.1e-07 EIF3I
WD40_WDHD1_1st PF24817 298 - 461 7.5e-12 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 372 - 459 7.6e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 386 - 452 7.8e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 389 - 465 3e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 389 - 455 5.9e-07 THOC3 beta-propeller domain
WD40_Prp19 PF24814 393 - 457 9.9e-09 Prp19 WD40 domain
WD40 PF00400 416 - 451 2.5e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 917
Acc16I TGCGCA 1 cut(s) 926
AccB7I CCANNNNNTGG 1 cut(s) 771
AccII CGCG 1 cut(s) 1136
AciI CCGC 2 cut(s) 53, 1195
AclI AACGTT 1 cut(s) 704
AclWI GGATC 3 cut(s) 352, 762, 997
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 3 cut(s) 425, 446, 799
AfaI GTAC 3 cut(s) 384, 851, 977
AfiI CCNNNNNNNGG 3 cut(s) 92, 628, 771
AflIII ACRYGT 2 cut(s) 499, 549
AgeI ACCGGT 1 cut(s) 1066
AgsI TTSAA 3 cut(s) 209, 453, 495
AjiI CACGTC 1 cut(s) 552
AjnI CCWGG 4 cut(s) 98, 853, 1118, 1150
AluBI AGCT 2 cut(s) 249, 469
AluI AGCT 2 cut(s) 249, 469
Alw21I GWGCWC 1 cut(s) 1036
Alw26I GTCTC 3 cut(s) 398, 578, 903
AlwI GGATC 3 cut(s) 352, 762, 997
AoxI GGCC 3 cut(s) 10, 184, 589
ApeKI GCWGC 3 cut(s) 645, 668, 939
ApoI RAATTY 3 cut(s) 425, 446, 799
AsiGI ACCGGT 1 cut(s) 1066
AspLEI GCGC 1 cut(s) 927
AspS9I GGNCC 3 cut(s) 10, 95, 109
AsuC2I CCSGG 1 cut(s) 673
AsuHPI GGTGA 3 cut(s) 273, 309, 1036
AsuII TTCGAA 2 cut(s) 146, 238
AsuNHI GCTAGC 1 cut(s) 308
AvaII GGWCC 2 cut(s) 95, 109
BalI TGGCCA 1 cut(s) 591
BarI GAAGNNNNNNTAC 2 cut(s) 475, 507
Bbv12I GWGCWC 1 cut(s) 1036
BbvI GCAGC 3 cut(s) 632, 680, 926
BciT130I CCWGG 4 cut(s) 100, 855, 1120, 1152
BciVI GTATCC 1 cut(s) 816
BclI TGATCA 3 cut(s) 886, 997, 1159
BcnI CCSGG 1 cut(s) 673
BcoDI GTCTC 3 cut(s) 398, 578, 903
BfaI CTAG 3 cut(s) 309, 795, 993
BfmI CTRYAG 1 cut(s) 1170
BfuI GTATCC 1 cut(s) 816
BisI GCNGC 3 cut(s) 646, 669, 940
BlsI GCNGC 3 cut(s) 647, 670, 941
Bme1390I CCNGG 5 cut(s) 100, 673, 855, 1120, 1152
Bme18I GGWCC 2 cut(s) 95, 109
BmgBI CACGTC 1 cut(s) 552
BmgT120I GGNCC 3 cut(s) 10, 95, 109
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 5 cut(s) 100, 673, 855, 1120, 1152
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 3 cut(s) 46, 478, 1096
BmtI GCTAGC 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 838
BpmI CTGGAG 2 cut(s) 936, 1173
Bpu14I TTCGAA 2 cut(s) 146, 238
BpuEI CTTGAG 2 cut(s) 271, 1020
BpuMI CCSGG 1 cut(s) 673
BsaI GGTCTC 2 cut(s) 398, 903
BsaJI CCNNGG 7 cut(s) 98, 99, 672, 809, 853, 894, 1119
BsaWI WCCGGW 1 cut(s) 1066
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 628, 771
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 6 cut(s) 112, 592, 833, 970, 991, 1033
BseBI CCWGG 4 cut(s) 100, 855, 1120, 1152
BseDI CCNNGG 7 cut(s) 98, 99, 672, 809, 853, 894, 1119
BseGI GGATG 7 cut(s) 61, 221, 849, 877, 1087, 1115, 1129
BseLI CCNNNNNNNGG 3 cut(s) 92, 628, 771
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 6 cut(s) 112, 592, 833, 970, 991, 1033
BseRI GAGGAG 1 cut(s) 1213
BseXI GCAGC 3 cut(s) 632, 680, 926
BseYI CCCAGC 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 1136
BshFI GGCC 3 cut(s) 12, 186, 591
BshTI ACCGGT 1 cut(s) 1066
BsiHKAI GWGCWC 1 cut(s) 1036
BsiSI CCGG 3 cut(s) 606, 672, 1067
BslFI GGGAC 3 cut(s) 45, 81, 699
BslI CCNNNNNNNGG 3 cut(s) 92, 628, 771
BsmAI GTCTC 3 cut(s) 398, 578, 903
BsmFI GGGAC 3 cut(s) 45, 81, 699
BsnI GGCC 3 cut(s) 12, 186, 591
Bso31I GGTCTC 2 cut(s) 398, 903
Bsp119I TTCGAA 2 cut(s) 146, 238
Bsp1286I GDGCHC 1 cut(s) 1036
Bsp68I TCGCGA 1 cut(s) 1136
BspACI CCGC 2 cut(s) 53, 1195
BspANI GGCC 3 cut(s) 12, 186, 591
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 1136
BspHI TCATGA 2 cut(s) 823, 1057
BspLI GGNNCC 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 312
BspPI GGATC 3 cut(s) 352, 762, 997
BspT104I TTCGAA 2 cut(s) 146, 238
BspTNI GGTCTC 2 cut(s) 398, 903
BsrFI RCCGGY 1 cut(s) 1066
BsrI ACTGG 6 cut(s) 112, 592, 833, 970, 991, 1033
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 7 cut(s) 98, 99, 672, 809, 853, 894, 1119
BssT1I CCWWGG 2 cut(s) 809, 894
Bst2UI CCWGG 4 cut(s) 100, 855, 1120, 1152
Bst4CI ACNGT 2 cut(s) 568, 1174
Bst6I CTCTTC 1 cut(s) 457
BstBI TTCGAA 2 cut(s) 146, 238
BstC8I GCNNGC 2 cut(s) 310, 666
BstDEI CTNAG 1 cut(s) 138
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 7 cut(s) 61, 221, 849, 877, 1087, 1115, 1129
BstFNI CGCG 1 cut(s) 1136
BstHHI GCGC 1 cut(s) 927
BstMAI GTCTC 3 cut(s) 398, 578, 903
BstMWI GCNNNNNNNGC 1 cut(s) 1040
BstNI CCWGG 4 cut(s) 100, 855, 1120, 1152
BstNSI RCATGY 2 cut(s) 316, 503
BstSCI CCNGG 5 cut(s) 98, 671, 853, 1118, 1150
BstSFI CTRYAG 1 cut(s) 1170
BstUI CGCG 1 cut(s) 1136
BstV1I GCAGC 3 cut(s) 632, 680, 926
BstX2I RGATCY 2 cut(s) 344, 989
BstYI RGATCY 2 cut(s) 344, 989
BsuI GTATCC 1 cut(s) 816
BsuRI GGCC 3 cut(s) 12, 186, 591
BtrI CACGTC 1 cut(s) 552
BtsCI GGATG 7 cut(s) 61, 221, 849, 877, 1087, 1115, 1129
BtsI GCAGTG 1 cut(s) 616
BtsIMutI CAGTG 4 cut(s) 616, 963, 984, 1179
BtuMI TCGCGA 1 cut(s) 1136
Cac8I GCNNGC 2 cut(s) 310, 666
CciI TCATGA 2 cut(s) 823, 1057
CfoI GCGC 1 cut(s) 927
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 3 cut(s) 10, 95, 109
CseI GACGC 1 cut(s) 651
Csp6I GTAC 3 cut(s) 383, 850, 976
CspAI ACCGGT 1 cut(s) 1066
CviAII CATG 8 cut(s) 65, 313, 386, 500, 824, 1001, 1058, 1102
CviQI GTAC 3 cut(s) 383, 850, 976
DdeI CTNAG 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 917
DseDI GACNNNNNNGTC 1 cut(s) 917
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 457
EciI GGCGGA 1 cut(s) 1210
Eco130I CCWWGG 2 cut(s) 809, 894
Eco31I GGTCTC 2 cut(s) 398, 903
Eco47I GGWCC 2 cut(s) 95, 109
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoRII CCWGG 4 cut(s) 98, 853, 1118, 1150
EcoT14I CCWWGG 2 cut(s) 809, 894
ErhI CCWWGG 2 cut(s) 809, 894
FaeI CATG 8 cut(s) 68, 316, 389, 503, 827, 1004, 1061, 1105
FaqI GGGAC 3 cut(s) 45, 81, 699
FatI CATG 8 cut(s) 64, 312, 385, 499, 823, 1000, 1057, 1101
FauNDI CATATG 2 cut(s) 130, 304
FbaI TGATCA 3 cut(s) 886, 997, 1159
Fnu4HI GCNGC 3 cut(s) 646, 669, 940
FokI GGATG 7 cut(s) 68, 228, 856, 864, 1074, 1102, 1136
Fsp4HI GCNGC 3 cut(s) 646, 669, 940
FspBI CTAG 3 cut(s) 309, 795, 993
FspI TGCGCA 1 cut(s) 926
GlaI GCGC 1 cut(s) 926
GluI GCNGC 3 cut(s) 646, 669, 940
GsaI CCCAGC 1 cut(s) 174
GsuI CTGGAG 2 cut(s) 936, 1173
HaeIII GGCC 3 cut(s) 12, 186, 591
HapII CCGG 3 cut(s) 606, 672, 1067
HgaI GACGC 1 cut(s) 651
HhaI GCGC 1 cut(s) 927
Hin1II CATG 8 cut(s) 68, 316, 389, 503, 827, 1004, 1061, 1105
Hin6I GCGC 1 cut(s) 925
HinP1I GCGC 1 cut(s) 925
HindIII AAGCTT 1 cut(s) 247
HinfI GANTC 1 cut(s) 359
HpaII CCGG 3 cut(s) 606, 672, 1067
HphI GGTGA 3 cut(s) 273, 309, 1036
Hpy166II GTNNAC 2 cut(s) 109, 562
Hpy188I TCNGA 4 cut(s) 228, 556, 681, 759
Hpy188III TCNNGA 8 cut(s) 30, 395, 655, 692, 795, 824, 1058, 1135
Hpy8I GTNNAC 2 cut(s) 109, 562
HpyAV CCTTC 4 cut(s) 223, 383, 689, 745
HpyCH4III ACNGT 2 cut(s) 568, 1174
HpyCH4IV ACGT 2 cut(s) 551, 704
HpyCH4V TGCA 6 cut(s) 128, 302, 368, 578, 1004, 1043
HpyF10VI GCNNNNNNNGC 1 cut(s) 1040
HpyF3I CTNAG 1 cut(s) 138
HpySE526I ACGT 2 cut(s) 551, 704
Hsp92II CATG 8 cut(s) 68, 316, 389, 503, 827, 1004, 1061, 1105
HspAI GCGC 1 cut(s) 925
Ksp22I TGATCA 3 cut(s) 886, 997, 1159
Lsp1109I GCAGC 3 cut(s) 632, 680, 926
LweI GCATC 3 cut(s) 46, 478, 1096
MaeI CTAG 3 cut(s) 309, 795, 993
MaeII ACGT 2 cut(s) 551, 704
MaeIII GTNAC 4 cut(s) 315, 652, 731, 1024
MboII GAAGA 4 cut(s) 339, 434, 474, 796
MflI RGATCY 2 cut(s) 344, 989
MhlI GDGCHC 1 cut(s) 1036
MlsI TGGCCA 1 cut(s) 591
MluCI AATT 7 cut(s) 22, 222, 261, 425, 446, 799, 933
MluNI TGGCCA 1 cut(s) 591
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 8 cut(s) 29, 33, 176, 542, 632, 802, 1191, 1194
Mox20I TGGCCA 1 cut(s) 591
MscI TGGCCA 1 cut(s) 591
MseI TTAA 6 cut(s) 71, 90, 444, 737, 863, 932
MslI CAYNNNNRTG 2 cut(s) 504, 1059
Msp20I TGGCCA 1 cut(s) 591
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 3 cut(s) 606, 672, 1067
MspR9I CCNGG 5 cut(s) 100, 673, 855, 1120, 1152
MvaI CCWGG 4 cut(s) 100, 855, 1120, 1152
MvnI CGCG 1 cut(s) 1136
MwoI GCNNNNNNNGC 1 cut(s) 1040
NciI CCSGG 1 cut(s) 673
NdeI CATATG 2 cut(s) 130, 304
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 8 cut(s) 68, 316, 389, 503, 827, 1004, 1061, 1105
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 3 cut(s) 315, 652, 1024
NruI TCGCGA 1 cut(s) 1136
NsbI TGCGCA 1 cut(s) 926
NspI RCATGY 2 cut(s) 316, 503
NspV TTCGAA 2 cut(s) 146, 238
PagI TCATGA 2 cut(s) 823, 1057
PasI CCCWGGG 1 cut(s) 99
PciI ACATGT 1 cut(s) 499
PflFI GACNNNGTC 1 cut(s) 358
PflMI CCANNNNNTGG 1 cut(s) 771
PfoI TCCNGGA 1 cut(s) 1150
PinAI ACCGGT 1 cut(s) 1066
PkrI GCNGC 3 cut(s) 647, 670, 941
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PscI ACATGT 1 cut(s) 499
Psp1406I AACGTT 1 cut(s) 704
Psp6I CCWGG 4 cut(s) 98, 853, 1118, 1150
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 4 cut(s) 98, 853, 1118, 1150
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 3 cut(s) 10, 95, 109
PsuI RGATCY 2 cut(s) 344, 989
PsyI GACNNNGTC 1 cut(s) 358
RruI TCGCGA 1 cut(s) 1136
RsaI GTAC 3 cut(s) 384, 851, 977
RsaNI GTAC 3 cut(s) 383, 850, 976
RseI CAYNNNNRTG 2 cut(s) 504, 1059
SaqAI TTAA 6 cut(s) 71, 90, 444, 737, 863, 932
SatI GCNGC 3 cut(s) 646, 669, 940
Sau96I GGNCC 3 cut(s) 10, 95, 109
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 5 cut(s) 100, 673, 855, 1120, 1152
SduI GDGCHC 1 cut(s) 1036
SfaNI GCATC 3 cut(s) 46, 478, 1096
SfcI CTRYAG 1 cut(s) 1170
SfuI TTCGAA 2 cut(s) 146, 238
SinI GGWCC 2 cut(s) 95, 109
SmiMI CAYNNNNRTG 2 cut(s) 504, 1059
SmlI CTYRAG 2 cut(s) 250, 1035
SmoI CTYRAG 2 cut(s) 250, 1035
Sse9I AATT 7 cut(s) 22, 222, 261, 425, 446, 799, 933
SsiI CCGC 2 cut(s) 53, 1195
SspI AATATT 1 cut(s) 435
SspMI CTAG 3 cut(s) 309, 795, 993
StyD4I CCNGG 5 cut(s) 98, 671, 853, 1118, 1150
StyI CCWWGG 2 cut(s) 809, 894
TaaI ACNGT 2 cut(s) 568, 1174
TaiI ACGT 2 cut(s) 554, 707
TaqI TCGA 3 cut(s) 146, 238, 396
TasI AATT 7 cut(s) 22, 222, 261, 425, 446, 799, 933
TatI WGTACW 2 cut(s) 382, 975
Tru1I TTAA 6 cut(s) 71, 90, 444, 737, 863, 932
Tru9I TTAA 6 cut(s) 71, 90, 444, 737, 863, 932
TscAI CASTG 4 cut(s) 616, 970, 991, 1179
TseFI GTSAC 3 cut(s) 315, 652, 1024
TseI GCWGC 3 cut(s) 645, 668, 939
Tsp45I GTSAC 3 cut(s) 315, 652, 1024
TspDTI ATGAA 5 cut(s) 36, 402, 435, 1077, 1104
TspGWI ACGGA 2 cut(s) 392, 796
TspRI CASTG 4 cut(s) 616, 970, 991, 1179
Tth111I GACNNNGTC 1 cut(s) 358
Van91I CCANNNNNTGG 1 cut(s) 771
VpaK11BI GGWCC 2 cut(s) 95, 109
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 3 cut(s) 425, 446, 799
XbaI TCTAGA 1 cut(s) 794
XceI RCATGY 2 cut(s) 316, 503
XspI CTAG 3 cut(s) 309, 795, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.