Rh4CG239900

LEC14B homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001751
Physical Location & Seq
Reverse (-)
48560782 .. 48565931
5150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG239900.1

Sequence Viewer

Length: 1416 bp
ATGAGTTACAGAAGGAGGTTTGGGAAAGATAATAGTGCCTGTGATAGTGGGAATGCTGTTGAAGGTTCTGCTTCAAGTGAAGAACCCAATGAAGCATCAAAGAGCCTTGATCATGAAGTGGCTCACCTCACTAAGCTTAGATCAAGACCCCATCAGCATTTGAGGGGGCTCTTGCCCGGAAAGTTGAGGTTACCGGTGTCAACACTGAAAATGTTGATGGGCCGTGAAAGTAATTATTCGGGGCGAGGGAGGTTTTTGTCAGCAGATGCTTGTCATGTTTTAAGCCGGTATCTGCTCATTAATGGTCCATGGGGGGTGGACCAGTTGAATAGCCCTGCATATGTTTCTCAGTTTTCGAATGATGGCTTGTTTTTTGTTGCTGGATTTCAGGGAGGTCATATTAGAATATATAATGTTGAAAAGGGATGGACAGTTCAGAAGGACATTCTAACCAAAAGCTTGAGATGGACAATTACCGATACATCTCTATCACCAGATCAACGATTTCTTGCATATGCTAGCATGTCACCTATTGTCCATATTGTCAATGCTGGATCTTCTACGACAGAGTCACTTGCAAACATTACGGAAATACATGAAGATCTGGATTTTTCTGTTGATTATGATGAAGAATTTGGAATATTCTCTGTTAAATTTTCAACTGATGGGCGAGAGCTTGTGGTTGGAAGTAGTGATGCTTCAATACATGTTTATGATCTCCAAGCAAACAAAGTTAGCCTCAAAATACCAGCGCATATGTCAGATGTAAACACAGTATGCTTTGCAGATGAGACTGGCCATCTCATATTTTCCGGCGGTGATGATAACCTCTGTAAGGTGTGGGATAGACGCTGCTTTATCACGAAAGGGCAAGCAGCTGGGGTTCTGATGGGACATCTTGAAGGCATAACGTTTATTGATAGTCGGGGAGATGGACGTTACTTAATATCAAATGGGAAGGATCAGACTACCAAACTTTGGGATATACGGAAGATGTCCTCTAGAGATATGTATCCAAGGATTACAGATCGTGACTGGGATTACAGGTGGATGGAGTACCCAGCTCGTTCTTCGAGACATCCCTTGGATCAGTCGTTGGCTACATATAGAGACCATTCAGTCCTGCGCACTTTAATTCGCTGTTACTTTTCTCCAGCATATAGCACTGGACAAAAGTACATCTACACTGGATCTAGTGATCATTCGGTCTATATTTATGATCTGGTGACTGGCGCTCAAGTTGCGAAACTCTTCCATCATGATGGACCTGTAAGAGATTGTAGTTGGCATCCTACATATCCCATGTTGGTCAGTTCTTCCTGGGATGGGAGTATCGTGAGATGGGAGTTTCCGGGAGATGATCAAGTTCCTACCCTGGTCAAGCCAAGAGTAAGGCGGAGGAGAGCTTTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

471

Amino Acids

53.26

Weight (kDa)

7.29

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 108 - 207 2e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 117 - 448 1.5e-30 WDR5 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 121 - 331 6.3e-06 WDR90/POC16, second beta-propeller
Beta-prop_TEP1_2nd PF25047 124 - 331 6.7e-09 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 127 - 281 3.4e-09 WDR36/Utp21 first beta-propeller
WDR55 PF24796 157 - 332 6.5e-15 WDR55
WD40_Prp19 PF24814 159 - 284 2.2e-10 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 178 - 282 9.9e-08 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 180 - 284 2.3e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 208 - 328 7.2e-13 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 209 - 337 3.7e-15 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 213 - 332 1.3e-18 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 214 - 282 3e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 214 - 347 2.1e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 215 - 337 2.1e-16 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 298 - 458 3e-10 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 386 - 451 1.1e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 386 - 462 4.8e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 390 - 454 4.7e-08 Prp19 WD40 domain
WD40 PF00400 413 - 448 1.2e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1118
Acc16I TGCGCA 1 cut(s) 1127
AccB7I CCANNNNNTGG 1 cut(s) 978
AciI CCGC 2 cut(s) 816, 1396
AclI AACGTT 1 cut(s) 911
AclWI GGATC 4 cut(s) 562, 969, 1095, 1198
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 2 cut(s) 632, 653
AfaI GTAC 2 cut(s) 1058, 1178
AfiI CCNNNNNNNGG 3 cut(s) 835, 978, 1326
AflIII ACRYGT 1 cut(s) 706
AgeI ACCGGT 1 cut(s) 193
AgsI TTSAA 7 cut(s) 62, 75, 328, 419, 660, 702, 902
AjnI CCWGG 2 cut(s) 1319, 1374
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 6 cut(s) 136, 459, 676, 878, 1064, 1406
AluI AGCT 6 cut(s) 136, 459, 676, 878, 1064, 1406
Alw26I GTCTC 3 cut(s) 785, 1069, 1104
AlwI GGATC 4 cut(s) 562, 969, 1095, 1198
AoxI GGCC 2 cut(s) 220, 796
ApeKI GCWGC 2 cut(s) 852, 875
ApoI RAATTY 2 cut(s) 632, 653
AseI ATTAAT 1 cut(s) 300
AsiGI ACCGGT 1 cut(s) 193
Asp700I GAANNNNTTC 1 cut(s) 1250
AspLEI GCGC 3 cut(s) 754, 1128, 1235
AspS9I GGNCC 4 cut(s) 220, 305, 319, 1265
AsuC2I CCSGG 2 cut(s) 177, 1353
AsuHPI GGTGA 5 cut(s) 116, 483, 519, 830, 1237
AsuII TTCGAA 1 cut(s) 356
AsuNHI GCTAGC 1 cut(s) 518
AvaII GGWCC 3 cut(s) 305, 319, 1265
BalI TGGCCA 1 cut(s) 798
BanII GRGCYC 1 cut(s) 171
BarI GAAGNNNNNNTAC 2 cut(s) 682, 714
BbvI GCAGC 2 cut(s) 839, 887
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 2 cut(s) 1321, 1376
BciVI GTATCC 1 cut(s) 1023
BclI TGATCA 3 cut(s) 109, 1198, 1360
BcnI CCSGG 2 cut(s) 177, 1353
BcoDI GTCTC 3 cut(s) 785, 1069, 1104
BfaI CTAG 3 cut(s) 519, 1002, 1194
BfoI RGCGCY 1 cut(s) 1236
BfuI GTATCC 1 cut(s) 1023
BglII AGATCT 1 cut(s) 601
BisI GCNGC 2 cut(s) 853, 876
BlsI GCNGC 2 cut(s) 854, 877
Bme1390I CCNGG 4 cut(s) 177, 1321, 1353, 1376
Bme18I GGWCC 3 cut(s) 305, 319, 1265
BmgT120I GGNCC 4 cut(s) 220, 305, 319, 1265
BmrFI CCNGG 4 cut(s) 177, 1321, 1353, 1376
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 4 cut(s) 104, 256, 685, 1297
BmtI GCTAGC 1 cut(s) 522
BmuI ACTGGG 1 cut(s) 1045
BpmI CTGGAG 1 cut(s) 1137
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 481, 1221
BpuMI CCSGG 2 cut(s) 177, 1353
BsaBI GATNNNNATC 1 cut(s) 1011
BsaI GGTCTC 1 cut(s) 1104
BsaJI CCNNGG 5 cut(s) 308, 1016, 1083, 1320, 1374
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 3 cut(s) 835, 978, 1326
Bse118I RCCGGY 2 cut(s) 193, 285
Bse1I ACTGG 6 cut(s) 322, 799, 1040, 1171, 1192, 1234
Bse8I GATNNNNATC 1 cut(s) 1011
BseBI CCWGG 2 cut(s) 1321, 1376
BseDI CCNNGG 5 cut(s) 308, 1016, 1083, 1320, 1374
BseGI GGATG 5 cut(s) 431, 1056, 1078, 1288, 1330
BseJI GATNNNNATC 1 cut(s) 1011
BseLI CCNNNNNNNGG 3 cut(s) 835, 978, 1326
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 6 cut(s) 322, 799, 1040, 1171, 1192, 1234
BseRI GAGGAG 1 cut(s) 1414
BseXI GCAGC 2 cut(s) 839, 887
BseYI CCCAGC 2 cut(s) 878, 1060
BshFI GGCC 2 cut(s) 222, 798
BshTI ACCGGT 1 cut(s) 193
BsiSI CCGG 5 cut(s) 177, 194, 286, 813, 1352
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 3 cut(s) 835, 978, 1326
BsmAI GTCTC 3 cut(s) 785, 1069, 1104
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 1 cut(s) 58
BsnI GGCC 2 cut(s) 222, 798
Bso31I GGTCTC 1 cut(s) 1104
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 171
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 2 cut(s) 816, 1396
BspANI GGCC 2 cut(s) 222, 798
BspCNI CTCAG 1 cut(s) 361
BspHI TCATGA 2 cut(s) 112, 1258
BspOI GCTAGC 1 cut(s) 522
BspPI GGATC 4 cut(s) 562, 969, 1095, 1198
BspT104I TTCGAA 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 1104
BsrFI RCCGGY 2 cut(s) 193, 285
BsrI ACTGG 6 cut(s) 322, 799, 1040, 1171, 1192, 1234
BssAI RCCGGY 2 cut(s) 193, 285
BssECI CCNNGG 5 cut(s) 308, 1016, 1083, 1320, 1374
BssT1I CCWWGG 3 cut(s) 308, 1016, 1083
Bst2UI CCWGG 2 cut(s) 1321, 1376
Bst4CI ACNGT 2 cut(s) 433, 775
Bst6I CTCTTC 1 cut(s) 1256
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 520, 873
BstDEI CTNAG 3 cut(s) 132, 137, 348
BstDSI CCRYGG 1 cut(s) 308
BstEII GGTNACC 1 cut(s) 189
BstENI CCTNNNNNAGG 1 cut(s) 833
BstF5I GGATG 5 cut(s) 431, 1056, 1078, 1288, 1330
BstH2I RGCGCY 1 cut(s) 1236
BstHHI GCGC 3 cut(s) 754, 1128, 1235
BstMAI GTCTC 3 cut(s) 785, 1069, 1104
BstMWI GCNNNNNNNGC 1 cut(s) 1241
BstNI CCWGG 2 cut(s) 1321, 1376
BstNSI RCATGY 2 cut(s) 526, 710
BstPI GGTNACC 1 cut(s) 189
BstSCI CCNGG 4 cut(s) 175, 1319, 1351, 1374
BstV1I GCAGC 2 cut(s) 839, 887
BstX2I RGATCY 3 cut(s) 554, 601, 1190
BstXI CCANNNNNNTGG 1 cut(s) 1262
BstYI RGATCY 3 cut(s) 554, 601, 1190
BsuI GTATCC 1 cut(s) 1023
BsuRI GGCC 2 cut(s) 222, 798
BtgI CCRYGG 1 cut(s) 308
BtsCI GGATG 5 cut(s) 431, 1056, 1078, 1288, 1330
BtsIMutI CAGTG 3 cut(s) 203, 1164, 1185
Cac8I GCNNGC 2 cut(s) 520, 873
CciI TCATGA 2 cut(s) 112, 1258
CfoI GCGC 3 cut(s) 754, 1128, 1235
Cfr10I RCCGGY 2 cut(s) 193, 285
Cfr13I GGNCC 4 cut(s) 220, 305, 319, 1265
CseI GACGC 1 cut(s) 858
Csp6I GTAC 2 cut(s) 1057, 1177
CspAI ACCGGT 1 cut(s) 193
CviAII CATG 8 cut(s) 113, 275, 309, 523, 596, 707, 1259, 1303
CviQI GTAC 2 cut(s) 1057, 1177
DdeI CTNAG 3 cut(s) 132, 137, 348
DrdI GACNNNNNNGTC 1 cut(s) 1118
DseDI GACNNNNNNGTC 1 cut(s) 1118
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 1256
EarI CTCTTC 1 cut(s) 1256
EciI GGCGGA 1 cut(s) 1411
Eco130I CCWWGG 3 cut(s) 308, 1016, 1083
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 1104
Eco47I GGWCC 3 cut(s) 305, 319, 1265
Eco91I GGTNACC 1 cut(s) 189
EcoNI CCTNNNNNAGG 1 cut(s) 833
EcoO65I GGTNACC 1 cut(s) 189
EcoRII CCWGG 2 cut(s) 1319, 1374
EcoT14I CCWWGG 3 cut(s) 308, 1016, 1083
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 3 cut(s) 308, 1016, 1083
FaeI CATG 8 cut(s) 116, 278, 312, 526, 599, 710, 1262, 1306
FaqI GGGAC 1 cut(s) 906
FatI CATG 8 cut(s) 112, 274, 308, 522, 595, 706, 1258, 1302
FauNDI CATATG 3 cut(s) 340, 514, 756
FbaI TGATCA 3 cut(s) 109, 1198, 1360
Fnu4HI GCNGC 2 cut(s) 853, 876
FokI GGATG 5 cut(s) 438, 1063, 1065, 1275, 1337
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 2 cut(s) 853, 876
FspBI CTAG 3 cut(s) 519, 1002, 1194
FspI TGCGCA 1 cut(s) 1127
GlaI GCGC 3 cut(s) 753, 1127, 1234
GluI GCNGC 2 cut(s) 853, 876
GsaI CCCAGC 2 cut(s) 882, 1064
GsuI CTGGAG 1 cut(s) 1137
HaeII RGCGCY 1 cut(s) 1236
HaeIII GGCC 2 cut(s) 222, 798
HapII CCGG 5 cut(s) 177, 194, 286, 813, 1352
HgaI GACGC 1 cut(s) 858
HhaI GCGC 3 cut(s) 754, 1128, 1235
Hin1II CATG 8 cut(s) 116, 278, 312, 526, 599, 710, 1262, 1306
Hin6I GCGC 3 cut(s) 752, 1126, 1233
HinP1I GCGC 3 cut(s) 752, 1126, 1233
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HindIII AAGCTT 2 cut(s) 134, 457
HinfI GANTC 1 cut(s) 569
HpaII CCGG 5 cut(s) 177, 194, 286, 813, 1352
HphI GGTGA 5 cut(s) 116, 483, 519, 830, 1237
Hpy166II GTNNAC 3 cut(s) 201, 319, 769
Hpy188I TCNGA 4 cut(s) 438, 763, 888, 966
Hpy8I GTNNAC 3 cut(s) 201, 319, 769
HpyAV CCTTC 5 cut(s) 6, 56, 433, 896, 952
HpyCH4III ACNGT 2 cut(s) 433, 775
HpyCH4IV ACGT 2 cut(s) 911, 937
HpyCH4V TGCA 4 cut(s) 338, 512, 578, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 1241
HpyF3I CTNAG 3 cut(s) 132, 137, 348
HpySE526I ACGT 2 cut(s) 911, 937
Hsp92II CATG 8 cut(s) 116, 278, 312, 526, 599, 710, 1262, 1306
HspAI GCGC 3 cut(s) 752, 1126, 1233
Ksp22I TGATCA 3 cut(s) 109, 1198, 1360
Lsp1109I GCAGC 2 cut(s) 839, 887
LweI GCATC 4 cut(s) 104, 256, 685, 1297
MaeI CTAG 3 cut(s) 519, 1002, 1194
MaeII ACGT 2 cut(s) 911, 937
MaeIII GTNAC 8 cut(s) 5, 189, 525, 570, 938, 1031, 1142, 1225
MboII GAAGA 8 cut(s) 92, 549, 611, 641, 1003, 1062, 1243, 1308
MflI RGATCY 3 cut(s) 554, 601, 1190
MhlI GDGCHC 1 cut(s) 171
MlsI TGGCCA 1 cut(s) 798
MluCI AATT 5 cut(s) 232, 471, 632, 653, 1134
MluNI TGGCCA 1 cut(s) 798
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 664
Mox20I TGGCCA 1 cut(s) 798
MroXI GAANNNNTTC 1 cut(s) 1250
MscI TGGCCA 1 cut(s) 798
MseI TTAA 5 cut(s) 281, 300, 651, 944, 1133
MslI CAYNNNNRTG 2 cut(s) 711, 1260
Msp20I TGGCCA 1 cut(s) 798
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 5 cut(s) 177, 194, 286, 813, 1352
MspR9I CCNGG 4 cut(s) 177, 1321, 1353, 1376
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 2 cut(s) 1321, 1376
MwoI GCNNNNNNNGC 1 cut(s) 1241
NciI CCSGG 2 cut(s) 177, 1353
NcoI CCATGG 1 cut(s) 308
NdeI CATATG 3 cut(s) 340, 514, 756
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 8 cut(s) 116, 278, 312, 526, 599, 710, 1262, 1306
NmuCI GTSAC 4 cut(s) 525, 570, 1031, 1225
NsbI TGCGCA 1 cut(s) 1127
NspI RCATGY 2 cut(s) 526, 710
NspV TTCGAA 1 cut(s) 356
PagI TCATGA 2 cut(s) 112, 1258
PciI ACATGT 1 cut(s) 706
PctI GAATGC 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 1250
PflFI GACNNNGTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 978
PfoI TCCNGGA 1 cut(s) 1351
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 2 cut(s) 854, 877
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
PscI ACATGT 1 cut(s) 706
PshBI ATTAAT 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 911
Psp6I CCWGG 2 cut(s) 1319, 1374
PspEI GGTNACC 1 cut(s) 189
PspFI CCCAGC 2 cut(s) 878, 1060
PspGI CCWGG 2 cut(s) 1319, 1374
PspPI GGNCC 4 cut(s) 220, 305, 319, 1265
PsuI RGATCY 3 cut(s) 554, 601, 1190
PsyI GACNNNGTC 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 2 cut(s) 1058, 1178
RsaNI GTAC 2 cut(s) 1057, 1177
RseI CAYNNNNRTG 2 cut(s) 711, 1260
SaqAI TTAA 5 cut(s) 281, 300, 651, 944, 1133
SatI GCNGC 2 cut(s) 853, 876
Sau96I GGNCC 4 cut(s) 220, 305, 319, 1265
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 4 cut(s) 177, 1321, 1353, 1376
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 4 cut(s) 104, 256, 685, 1297
SfuI TTCGAA 1 cut(s) 356
SinI GGWCC 3 cut(s) 305, 319, 1265
SmiMI CAYNNNNRTG 2 cut(s) 711, 1260
SmlI CTYRAG 2 cut(s) 460, 1236
SmoI CTYRAG 2 cut(s) 460, 1236
Sse9I AATT 5 cut(s) 232, 471, 632, 653, 1134
SsiI CCGC 2 cut(s) 816, 1396
SspI AATATT 1 cut(s) 642
SspMI CTAG 3 cut(s) 519, 1002, 1194
StyD4I CCNGG 4 cut(s) 175, 1319, 1351, 1374
StyI CCWWGG 3 cut(s) 308, 1016, 1083
TaaI ACNGT 2 cut(s) 433, 775
TaiI ACGT 2 cut(s) 914, 940
TaqI TCGA 2 cut(s) 356, 1073
TaqII GACCGA 1 cut(s) 1195
TasI AATT 5 cut(s) 232, 471, 632, 653, 1134
TatI WGTACW 1 cut(s) 1176
Tru1I TTAA 5 cut(s) 281, 300, 651, 944, 1133
Tru9I TTAA 5 cut(s) 281, 300, 651, 944, 1133
TscAI CASTG 3 cut(s) 210, 1171, 1192
TseFI GTSAC 4 cut(s) 525, 570, 1031, 1225
TseI GCWGC 2 cut(s) 852, 875
Tsp45I GTSAC 4 cut(s) 525, 570, 1031, 1225
TspDTI ATGAA 4 cut(s) 105, 129, 612, 642
TspGWI ACGGA 2 cut(s) 602, 1003
TspRI CASTG 3 cut(s) 210, 1171, 1192
Tth111I GACNNNGTC 1 cut(s) 568
Van91I CCANNNNNTGG 1 cut(s) 978
VpaK11BI GGWCC 3 cut(s) 305, 319, 1265
VspI ATTAAT 1 cut(s) 300
XagI CCTNNNNNAGG 1 cut(s) 833
XapI RAATTY 2 cut(s) 632, 653
XbaI TCTAGA 1 cut(s) 1001
XceI RCATGY 2 cut(s) 526, 710
XmnI GAANNNNTTC 1 cut(s) 1250
XspI CTAG 3 cut(s) 519, 1002, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.