MD16G1177700.v1.1

LEC14B homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001751
Physical Location & Seq
Forward (+)
15123616 .. 15133918
10303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1177700.v1.1.491

Sequence Viewer

Length: 1425 bp
ATGAGTTACATAGCGAGGTTTGGTAAAGATAATAGCGCCTGTGATACCGGAAATTCTGCTGAAGGTTCGGGTTCAAGTAAAGGACCTGATGAAGTATCCAATGATTTTGATCATGAAATTGCTCAACTTACCAAGCATAAATCAAGACCCCATCAACTTTTGCGCGGGGATATGCCTGGGAAGTTGAGGTTACCGGTTTCAACGATGAAAATGCTGGTCGGTCGCGAAAGTAATCATTCAGGGCGGGGGAGGTTCTCATCGGCAGATTGTTGCCATGTTTTGAGCCGGTATCTGCCCCTAAATGGTCCTTGGGGGGTGGACCAGTCCAGAAGCCCTGCTTATGTTTCTCAATTTTCCAATGATGGTTTGTTTTTTGTTGCTGGATTTCAGGGAGGCCATATTAGAATATATAATGTTGAAAAGGGATGGAAAGTTCAGAAGGATATCCTGACCCAAAGCTTGAGATGGACAATTACCGATACGTCTCTATCACCGGACCAACGTTATCTAGTTTATGCTAGCATGACACCGGTTGTCAATATTGTGAATGTGGAATCTTCTAAGACAGAGTCACTTGCAAACGTTACGGAAATTCATGGAGGTCTAGATTTTTCTATTGATGACGATGAGGATGAGTTTGGAATTTTCTCAGTTAAATTTTCAACTGATGGGCGAGAGCTTGTAGCTGCAAGTAGTGATGCTTCAATATATGTTTATGATCTACAAGAAAATAAAGTTAGCCTCCGAATACCAGCACACCAGTCTGATGTAAACACTGTATGCTTTGCTGATGAGAGCGGCCATCTCATATATTCTGGCAGTGACGACAACCTCTGTAAGGTGTGGGATAGACGCTGCTTAATGACGAAAGGAAAAGCAGCTGGGGTCCTGATGGGACATCTTGAAGGTGTTACATTTATTGATAGTCGGGGAGATGGGCGTTACTTGATATCAAACGGGAAGGACCAGACTACCAAACTCTGGGATATAAGAAAGATGTCCTCTACAGCCATTTATCCAAGGCGTACATATCGTGACTGGGATTACAGGTGGATGGAGTACCCATCTCATGCAACAACTTTGAAACATCCCAATGATCAATCCTTGGCTACATATAGAGGCCATGCAGTCCTGCGCACTTTAATTCGCTGTTACTTTTCTCCAGCATATAGTACCGGACAGAAGTACATCTACACTGGCTCAAGTGATCATTCTGTCTATATATATGATCTGGTGACTGGAGCTCAAGTTGCGAGACTAGACCATCATGAAGGACCTGTAAGAGACTGTAGTTGGCATCCTCTATATCCCATTTTGGTCAGCTCTTCTTGGGATGGGACCGTTGCCCGATGGGAATTTCCTGGTGATGACCAAGTTCCCACCCTGATGAGGCCAAAATTACGCCCAAGGAGAGTCTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

475

Amino Acids

53.37

Weight (kDa)

6.78

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 110 - 207 6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 120 - 332 1e-11 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 127 - 282 6.4e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 133 - 330 1.6e-18 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 156 - 286 2.5e-12 Prp19 WD40 domain
WDR55 PF24796 157 - 332 9.5e-15 WDR55
WD40_MABP1-WDR62_2nd PF24782 176 - 283 1e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 213 - 337 3.7e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 214 - 334 9.8e-21 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 215 - 336 4.1e-18 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 215 - 334 2.4e-10 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 215 - 349 5.5e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 215 - 335 4.8e-17 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 216 - 283 2.1e-06 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 225 - 336 1.2e-09 TEP-1 second beta-propeller
WD40 PF00400 249 - 283 2.9e-06 WD domain, G-beta repeat
EIF3I PF24805 253 - 447 1.9e-08 EIF3I
Beta-prop_WDR5 PF25175 365 - 451 9.4e-11 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 389 - 454 1.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 389 - 463 1.1e-10 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 391 - 459 2.3e-08 WDHD1 first WD40 domain
WD40_Prp19 PF24814 393 - 457 8.6e-09 Prp19 WD40 domain
WD40 PF00400 416 - 451 2.7e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1136
AccB7I CCANNNNNTGG 1 cut(s) 981
AccBSI CCGCTC 1 cut(s) 798
AccII CGCG 2 cut(s) 165, 225
AciI CCGC 3 cut(s) 165, 244, 798
AclI AACGTT 2 cut(s) 502, 582
AcoI YGGCCR 1 cut(s) 799
AcsI RAATTY 5 cut(s) 52, 591, 642, 656, 1355
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 4 cut(s) 1027, 1061, 1174, 1187
AfiI CCNNNNNNNGG 4 cut(s) 302, 838, 981, 1389
AgeI ACCGGT 2 cut(s) 193, 529
AgsI TTSAA 7 cut(s) 75, 201, 419, 663, 705, 905, 1084
AjnI CCWGG 2 cut(s) 175, 1360
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 6 cut(s) 459, 679, 686, 881, 1244, 1323
AluI AGCT 6 cut(s) 459, 679, 686, 881, 1244, 1323
Alw21I GWGCWC 1 cut(s) 1246
Alw26I GTCTC 3 cut(s) 489, 1249, 1278
AoxI GGCC 4 cut(s) 394, 799, 1120, 1391
ApeKI GCWGC 3 cut(s) 686, 855, 878
ApoI RAATTY 5 cut(s) 52, 591, 642, 656, 1355
AsiGI ACCGGT 2 cut(s) 193, 529
AspLEI GCGC 3 cut(s) 38, 165, 1137
AspS9I GGNCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
AsuHPI GGTGA 3 cut(s) 483, 1246, 1376
AsuNHI GCTAGC 1 cut(s) 518
AvaII GGWCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
BanII GRGCYC 1 cut(s) 1246
BarI GAAGNNNNNNTAC 4 cut(s) 685, 717, 1175, 1207
BbsI GAAGAC 1 cut(s) 1408
Bbv12I GWGCWC 1 cut(s) 1246
BbvI GCAGC 3 cut(s) 673, 842, 890
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 2 cut(s) 177, 1362
BciVI GTATCC 1 cut(s) 106
BclI TGATCA 3 cut(s) 109, 1096, 1207
BcoDI GTCTC 3 cut(s) 489, 1249, 1278
BfaI CTAG 4 cut(s) 509, 519, 605, 1259
BfmI CTRYAG 2 cut(s) 1005, 1288
BfoI RGCGCY 1 cut(s) 39
BfuI GTATCC 1 cut(s) 106
BisI GCNGC 4 cut(s) 687, 799, 856, 879
BlsI GCNGC 4 cut(s) 688, 800, 857, 880
Bme1390I CCNGG 2 cut(s) 177, 1362
Bme18I GGWCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
BmgT120I GGNCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
BmiI GGNNCC 2 cut(s) 887, 1339
BmrFI CCNGG 2 cut(s) 177, 1362
BmrI ACTGGG 1 cut(s) 1048
BmsI GCATC 2 cut(s) 688, 1306
BmtI GCTAGC 1 cut(s) 522
BmuI ACTGGG 1 cut(s) 1048
BpiI GAAGAC 1 cut(s) 1408
BpmI CTGGAG 2 cut(s) 1146, 1260
BpuEI CTTGAG 3 cut(s) 481, 1186, 1230
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 5 cut(s) 176, 308, 1019, 1104, 1406
BsaWI WCCGGW 5 cut(s) 47, 193, 493, 529, 1175
BsaXI ACNNNNNCTCC 2 cut(s) 1233, 1263
Bsc4I CCNNNNNNNGG 4 cut(s) 302, 838, 981, 1389
Bse118I RCCGGY 3 cut(s) 193, 285, 529
Bse1I ACTGG 5 cut(s) 322, 760, 1043, 1201, 1243
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 2 cut(s) 177, 1362
BseDI CCNNGG 5 cut(s) 176, 308, 1019, 1104, 1406
BseGI GGATG 6 cut(s) 431, 637, 1059, 1087, 1297, 1339
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 4 cut(s) 302, 838, 981, 1389
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 5 cut(s) 322, 760, 1043, 1201, 1243
BseXI GCAGC 3 cut(s) 673, 842, 890
BseYI CCCAGC 1 cut(s) 881
Bsh1236I CGCG 2 cut(s) 165, 225
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 4 cut(s) 396, 801, 1122, 1393
BshTI ACCGGT 2 cut(s) 193, 529
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 1246
BsiSI CCGG 6 cut(s) 48, 194, 286, 494, 530, 1176
BslFI GGGAC 2 cut(s) 909, 1351
BslI CCNNNNNNNGG 4 cut(s) 302, 838, 981, 1389
BsmAI GTCTC 3 cut(s) 489, 1249, 1278
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 2 cut(s) 909, 1351
BsnI GGCC 4 cut(s) 396, 801, 1122, 1393
Bsp1286I GDGCHC 1 cut(s) 1246
Bsp143I GATC 5 cut(s) 109, 718, 1096, 1207, 1228
Bsp68I TCGCGA 1 cut(s) 225
BspACI CCGC 3 cut(s) 165, 244, 798
BspANI GGCC 4 cut(s) 396, 801, 1122, 1393
BspCNI CTCAG 1 cut(s) 662
BspFNI CGCG 2 cut(s) 165, 225
BspHI TCATGA 2 cut(s) 112, 1267
BspLI GGNNCC 2 cut(s) 887, 1339
BspOI GCTAGC 1 cut(s) 522
BspQI GCTCTTC 1 cut(s) 1330
BsrBI CCGCTC 1 cut(s) 798
BsrFI RCCGGY 3 cut(s) 193, 285, 529
BsrI ACTGG 5 cut(s) 322, 760, 1043, 1201, 1243
BssAI RCCGGY 3 cut(s) 193, 285, 529
BssECI CCNNGG 5 cut(s) 176, 308, 1019, 1104, 1406
BssMI GATC 5 cut(s) 109, 718, 1096, 1207, 1228
BssT1I CCWWGG 4 cut(s) 308, 1019, 1104, 1406
Bst2UI CCWGG 2 cut(s) 177, 1362
Bst4CI ACNGT 3 cut(s) 778, 1289, 1342
Bst6I CTCTTC 1 cut(s) 1330
BstC8I GCNNGC 1 cut(s) 520
BstDEI CTNAG 2 cut(s) 561, 649
BstEII GGTNACC 1 cut(s) 189
BstENI CCTNNNNNAGG 1 cut(s) 836
BstF5I GGATG 6 cut(s) 431, 637, 1059, 1087, 1297, 1339
BstFNI CGCG 2 cut(s) 165, 225
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 3 cut(s) 38, 165, 1137
BstKTI GATC 5 cut(s) 112, 721, 1099, 1210, 1231
BstMAI GTCTC 3 cut(s) 489, 1249, 1278
BstMBI GATC 5 cut(s) 109, 718, 1096, 1207, 1228
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 1 cut(s) 1250
BstNI CCWGG 2 cut(s) 177, 1362
BstPI GGTNACC 1 cut(s) 189
BstSCI CCNGG 2 cut(s) 175, 1360
BstSFI CTRYAG 2 cut(s) 1005, 1288
BstUI CGCG 2 cut(s) 165, 225
BstV1I GCAGC 3 cut(s) 673, 842, 890
BstV2I GAAGAC 1 cut(s) 1408
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 4 cut(s) 396, 801, 1122, 1393
BtsCI GGATG 6 cut(s) 431, 637, 1059, 1087, 1297, 1339
BtsI GCAGTG 1 cut(s) 826
BtsIMutI CAGTG 3 cut(s) 774, 826, 1194
BtuMI TCGCGA 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 520
CciI TCATGA 2 cut(s) 112, 1267
CfoI GCGC 3 cut(s) 38, 165, 1137
Cfr10I RCCGGY 3 cut(s) 193, 285, 529
Cfr13I GGNCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
CseI GACGC 1 cut(s) 861
Csp6I GTAC 4 cut(s) 1026, 1060, 1173, 1186
CspAI ACCGGT 2 cut(s) 193, 529
CviAII CATG 7 cut(s) 113, 275, 523, 596, 1070, 1124, 1268
CviQI GTAC 4 cut(s) 1026, 1060, 1173, 1186
DdeI CTNAG 2 cut(s) 561, 649
DpnI GATC 5 cut(s) 111, 720, 1098, 1209, 1230
DpnII GATC 5 cut(s) 109, 718, 1096, 1207, 1228
EaeI YGGCCR 1 cut(s) 799
Eam1104I CTCTTC 1 cut(s) 1330
EarI CTCTTC 1 cut(s) 1330
Ecl136II GAGCTC 1 cut(s) 1244
Eco130I CCWWGG 4 cut(s) 308, 1019, 1104, 1406
Eco24I GRGCYC 1 cut(s) 1246
Eco32I GATATC 2 cut(s) 445, 951
Eco47I GGWCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
Eco53kI GAGCTC 1 cut(s) 1244
Eco57I CTGAAG 1 cut(s) 81
Eco91I GGTNACC 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 1244
EcoNI CCTNNNNNAGG 1 cut(s) 836
EcoO109I RGGNCCY 3 cut(s) 83, 886, 1274
EcoO65I GGTNACC 1 cut(s) 189
EcoRII CCWGG 2 cut(s) 175, 1360
EcoRV GATATC 2 cut(s) 445, 951
EcoT14I CCWWGG 4 cut(s) 308, 1019, 1104, 1406
EcoT38I GRGCYC 1 cut(s) 1246
ErhI CCWWGG 4 cut(s) 308, 1019, 1104, 1406
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 7 cut(s) 116, 278, 526, 599, 1073, 1127, 1271
FalI AAGNNNNNCTT 2 cut(s) 322, 354
FaqI GGGAC 2 cut(s) 909, 1351
FatI CATG 7 cut(s) 112, 274, 522, 595, 1069, 1123, 1267
FauI CCCGC 2 cut(s) 158, 237
FbaI TGATCA 3 cut(s) 109, 1096, 1207
Fnu4HI GCNGC 4 cut(s) 687, 799, 856, 879
FokI GGATG 6 cut(s) 438, 644, 1066, 1074, 1284, 1346
FriOI GRGCYC 1 cut(s) 1246
Fsp4HI GCNGC 4 cut(s) 687, 799, 856, 879
FspBI CTAG 4 cut(s) 509, 519, 605, 1259
FspI TGCGCA 1 cut(s) 1136
GlaI GCGC 3 cut(s) 37, 164, 1136
GluI GCNGC 4 cut(s) 687, 799, 856, 879
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 2 cut(s) 1146, 1260
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 4 cut(s) 396, 801, 1122, 1393
HapII CCGG 6 cut(s) 48, 194, 286, 494, 530, 1176
HgaI GACGC 1 cut(s) 861
HhaI GCGC 3 cut(s) 38, 165, 1137
Hin1II CATG 7 cut(s) 116, 278, 526, 599, 1073, 1127, 1271
Hin6I GCGC 3 cut(s) 36, 163, 1135
HinP1I GCGC 3 cut(s) 36, 163, 1135
HindIII AAGCTT 1 cut(s) 457
HinfI GANTC 3 cut(s) 554, 569, 1413
HpaII CCGG 6 cut(s) 48, 194, 286, 494, 530, 1176
HphI GGTGA 3 cut(s) 483, 1246, 1376
Hpy166II GTNNAC 2 cut(s) 319, 772
Hpy188I TCNGA 3 cut(s) 438, 746, 766
Hpy8I GTNNAC 2 cut(s) 319, 772
HpyAV CCTTC 5 cut(s) 56, 433, 899, 955, 1265
HpyCH4III ACNGT 3 cut(s) 778, 1289, 1342
HpyCH4IV ACGT 3 cut(s) 482, 502, 582
HpyCH4V TGCA 4 cut(s) 578, 689, 1073, 1127
HpyF10VI GCNNNNNNNGC 1 cut(s) 1250
HpyF3I CTNAG 2 cut(s) 561, 649
HpySE526I ACGT 3 cut(s) 482, 502, 582
Hsp92II CATG 7 cut(s) 116, 278, 526, 599, 1073, 1127, 1271
HspAI GCGC 3 cut(s) 36, 163, 1135
Ksp22I TGATCA 3 cut(s) 109, 1096, 1207
Kzo9I GATC 5 cut(s) 109, 718, 1096, 1207, 1228
LguI GCTCTTC 1 cut(s) 1330
LmnI GCTCC 1 cut(s) 1241
Lsp1109I GCAGC 3 cut(s) 673, 842, 890
LweI GCATC 2 cut(s) 688, 1306
MaeI CTAG 4 cut(s) 509, 519, 605, 1259
MaeII ACGT 3 cut(s) 482, 502, 582
MalI GATC 5 cut(s) 111, 720, 1098, 1209, 1230
MbiI CCGCTC 1 cut(s) 798
MboI GATC 5 cut(s) 109, 718, 1096, 1207, 1228
MboII GAAGA 3 cut(s) 549, 1317, 1408
MhlI GDGCHC 1 cut(s) 1246
MlyI GAGTC 2 cut(s) 578, 1422
MseI TTAA 3 cut(s) 654, 860, 1142
MslI CAYNNNNRTG 2 cut(s) 1092, 1385
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 6 cut(s) 48, 194, 286, 494, 530, 1176
MspR9I CCNGG 2 cut(s) 177, 1362
MvaI CCWGG 2 cut(s) 177, 1362
MvnI CGCG 2 cut(s) 165, 225
MwoI GCNNNNNNNGC 1 cut(s) 1250
NdeII GATC 5 cut(s) 109, 718, 1096, 1207, 1228
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 7 cut(s) 116, 278, 526, 599, 1073, 1127, 1271
NlaIV GGNNCC 2 cut(s) 887, 1339
NmuCI GTSAC 4 cut(s) 570, 821, 1034, 1234
NruI TCGCGA 1 cut(s) 225
NsbI TGCGCA 1 cut(s) 1136
PagI TCATGA 2 cut(s) 112, 1267
PciSI GCTCTTC 1 cut(s) 1330
PfeI GAWTC 1 cut(s) 554
PflFI GACNNNGTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 981
PinAI ACCGGT 2 cut(s) 193, 529
PkrI GCNGC 4 cut(s) 688, 800, 857, 880
PleI GAGTC 2 cut(s) 577, 1421
PpsI GAGTC 2 cut(s) 577, 1421
PpuMI RGGWCCY 3 cut(s) 83, 886, 1274
Psp124BI GAGCTC 1 cut(s) 1246
Psp1406I AACGTT 2 cut(s) 502, 582
Psp5II RGGWCCY 3 cut(s) 83, 886, 1274
Psp6I CCWGG 2 cut(s) 175, 1360
PspEI GGTNACC 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 881
PspGI CCWGG 2 cut(s) 175, 1360
PspN4I GGNNCC 2 cut(s) 887, 1339
PspPI GGNCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
PspPPI RGGWCCY 3 cut(s) 83, 886, 1274
PsyI GACNNNGTC 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 881
RruI TCGCGA 1 cut(s) 225
RsaI GTAC 4 cut(s) 1027, 1061, 1174, 1187
RsaNI GTAC 4 cut(s) 1026, 1060, 1173, 1186
RseI CAYNNNNRTG 2 cut(s) 1092, 1385
SacI GAGCTC 1 cut(s) 1246
SapI GCTCTTC 1 cut(s) 1330
SaqAI TTAA 3 cut(s) 654, 860, 1142
SatI GCNGC 4 cut(s) 687, 799, 856, 879
Sau3AI GATC 5 cut(s) 109, 718, 1096, 1207, 1228
Sau96I GGNCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
SchI GAGTC 2 cut(s) 578, 1422
ScrFI CCNGG 2 cut(s) 177, 1362
SduI GDGCHC 1 cut(s) 1246
SfaNI GCATC 2 cut(s) 688, 1306
SfcI CTRYAG 2 cut(s) 1005, 1288
SinI GGWCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
SmiMI CAYNNNNRTG 2 cut(s) 1092, 1385
SmlI CTYRAG 3 cut(s) 460, 1201, 1245
SmoI CTYRAG 3 cut(s) 460, 1201, 1245
SsiI CCGC 3 cut(s) 165, 244, 798
SspI AATATT 1 cut(s) 541
SspMI CTAG 4 cut(s) 509, 519, 605, 1259
SstI GAGCTC 1 cut(s) 1246
StyD4I CCNGG 2 cut(s) 175, 1360
StyI CCWWGG 4 cut(s) 308, 1019, 1104, 1406
TaaI ACNGT 3 cut(s) 778, 1289, 1342
TaiI ACGT 3 cut(s) 485, 505, 585
TaqII GACCGA 1 cut(s) 209
TatI WGTACW 1 cut(s) 1185
TauI GCSGC 1 cut(s) 801
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 3 cut(s) 654, 860, 1142
Tru9I TTAA 3 cut(s) 654, 860, 1142
TscAI CASTG 3 cut(s) 781, 826, 1201
TseFI GTSAC 4 cut(s) 570, 821, 1034, 1234
TseI GCWGC 3 cut(s) 686, 855, 878
Tsp45I GTSAC 4 cut(s) 570, 821, 1034, 1234
TspDTI ATGAA 5 cut(s) 105, 129, 221, 584, 1284
TspGWI ACGGA 1 cut(s) 602
TspRI CASTG 3 cut(s) 781, 826, 1201
Tth111I GACNNNGTC 1 cut(s) 568
Van91I CCANNNNNTGG 1 cut(s) 981
VpaK11BI GGWCC 8 cut(s) 83, 305, 319, 496, 886, 964, 1274, 1338
XagI CCTNNNNNAGG 1 cut(s) 836
XapI RAATTY 5 cut(s) 52, 591, 642, 656, 1355
XbaI TCTAGA 1 cut(s) 604
XspI CTAG 4 cut(s) 509, 519, 605, 1259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.