Prupe.1G145000_v2.0.a1

LEC14B homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001751
Physical Location & Seq
Forward (+)
11387427 .. 11392694
5268 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G145000.1

Sequence Viewer

Length: 1434 bp
ATGAGTTACAGAACGAGGTTTGGAAAAGATAATAGCGCTTGTGATAGTGGAAATGCTGTTGAAGGTTCTGGTTCAAGTAAAGGACCCAATGAAGTATCAAATGATTTTGATCATGAAATTGCTCAACTCACTAAGCATAGATCAAGACCCCATCAGCTTTTGAGCCAGGACATGCCTGGAAAGTCAAGGTTACTGGTTTCAACAATGAAAATGTTGGTTGGTCGTGAAAGTAATCATTCAGGACGCGGGAGATTCTCGTCTGCTGACGGTTGCCATGTTTTGAGCCGGTATCTGCCCATCAATGGTCCTTGGGGGGTGGACGAGTCAACAAGTCCTGCTTATGTTTCTCAATTTTCAAATGATGGTTTGTTTTTTGTTGCTGGATTTCAGGGAGGCCATATTAAAATATATAATGTTGATAAGGGATGGAAAGTTCAGAAGGACATCCTAACCAAAAGCTTGAGATGGACAATTACTGATACATCTCTATCGCCAGATCAACGTTATCTTGTTTATGCTAGCATGACACCCACTGTCAATATTGTCAATGTTGGATCTTCTATGACAGAGTCACTTGCAAATGTTACGGAAATTCATGAAGGTCTGGATTTTTCTGTTGATGGTGATGAGGACGAATTTGGAATTTTCTCAGTTAGATTTTCAACTGATGGGCGAGAGCTTGTAGCTGCAAGTAGTGATGCTTCAATATATGTTTATGATCTCCAAGCAAATAAAGTTAACCTCCGAATACCAGCACACTCGTCTGATGTAAACACTGTTTGCTTTGCGGATGAGACTGGACATCTCATATATTCTGGCAGTGACGATAATCTCTGTAAGGTTTGGGATAGACGCTGCTTAATCACAAAAGGACAAGCAGCTGGGGTCCTGATGGGACATCTTGAAGGTGTTACATTTCTTGATAGTCGGGGAGATGGGCGTTACTTAATATCAAATGGGAAGGACCAGACTACCAAACTCTGGGATATAAGAAAGATGTCCTCTAGAGCCATGTACAGCCCAAGGCTTAGAGATCATGACTGGGATTACAGATGGATGGAGTACCCAGCTCATGCAAAAACTTTGAAACATCCAAATGATCAGTCATTGGCTACATATAGAGGCCATGGAGTCCTTCGTACTTTAATTCGCTGTTACTTTTCTCCAGCATATAGTACTGGACAAAAGTACATCTACACTGGATCTAGTGATCATTGTGTCTATATATATGATCTGGTGACCGGAGCTCAAGTTGCGAGACTCAACCATCACGAAGGACCTGTAAGAGACTGTAGTTGGCATCCTCTCTATCCGATGTTGGTCAGCTCTTCTTGGGATGGGACGATTGCCAGATGGGAATTTCCTGGGGATGACCAAGTACCCACCCTGGAGAGGCCGAGAGCACGCCGGAGGAGAGGCTACTATAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

478

Amino Acids

53.64

Weight (kDa)

7.01

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 981
AccII CGCG 1 cut(s) 246
AciI CCGC 2 cut(s) 246, 788
AclI AACGTT 1 cut(s) 502
AclWI GGATC 2 cut(s) 562, 1210
AcsI RAATTY 4 cut(s) 591, 635, 642, 1358
AfaI GTAC 6 cut(s) 1016, 1064, 1141, 1177, 1190, 1380
AfeI AGCGCT 1 cut(s) 37
AfiI CCNNNNNNNGG 3 cut(s) 302, 981, 1392
AgsI TTSAA 8 cut(s) 62, 75, 201, 357, 663, 705, 905, 1087
AjnI CCWGG 4 cut(s) 165, 175, 1363, 1386
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 8 cut(s) 157, 459, 679, 686, 881, 1070, 1247, 1326
AluI AGCT 8 cut(s) 157, 459, 679, 686, 881, 1070, 1247, 1326
Alw21I GWGCWC 2 cut(s) 1249, 1405
Alw26I GTCTC 3 cut(s) 788, 1252, 1281
AlwI GGATC 2 cut(s) 562, 1210
Aor51HI AGCGCT 1 cut(s) 37
AoxI GGCC 3 cut(s) 394, 1123, 1394
ApeKI GCWGC 3 cut(s) 686, 855, 878
ApoI RAATTY 4 cut(s) 591, 635, 642, 1358
ArsI GACNNNNNNTTYG 2 cut(s) 1088, 1120
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 5 cut(s) 83, 305, 886, 964, 1277
AsuHPI GGTGA 2 cut(s) 635, 1249
AsuNHI GCTAGC 1 cut(s) 518
AvaII GGWCC 5 cut(s) 83, 305, 886, 964, 1277
BanII GRGCYC 1 cut(s) 1249
BarI GAAGNNNNNNTAC 2 cut(s) 685, 717
Bbv12I GWGCWC 2 cut(s) 1249, 1405
BbvI GCAGC 3 cut(s) 673, 842, 890
BciT130I CCWGG 4 cut(s) 167, 177, 1365, 1388
BclI TGATCA 3 cut(s) 109, 1099, 1210
BcoDI GTCTC 3 cut(s) 788, 1252, 1281
BfaI CTAG 3 cut(s) 519, 1005, 1206
BfmI CTRYAG 1 cut(s) 1291
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 3 cut(s) 687, 856, 879
BlsI GCNGC 3 cut(s) 688, 857, 880
BmcAI AGTACT 1 cut(s) 1177
Bme1390I CCNGG 4 cut(s) 167, 177, 1365, 1388
Bme18I GGWCC 5 cut(s) 83, 305, 886, 964, 1277
BmgT120I GGNCC 5 cut(s) 83, 305, 886, 964, 1277
BmiI GGNNCC 2 cut(s) 85, 887
BmrFI CCNGG 4 cut(s) 167, 177, 1365, 1388
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 2 cut(s) 688, 1309
BmtI GCTAGC 1 cut(s) 522
BmuI ACTGGG 1 cut(s) 1051
BpmI CTGGAG 2 cut(s) 1149, 1409
BpuEI CTTGAG 2 cut(s) 481, 1233
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 5 cut(s) 308, 1022, 1126, 1364, 1386
BsaWI WCCGGW 1 cut(s) 1241
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 981, 1392
Bse118I RCCGGY 1 cut(s) 285
Bse1I ACTGG 5 cut(s) 198, 802, 1046, 1183, 1204
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 4 cut(s) 167, 177, 1365, 1388
BseDI CCNNGG 5 cut(s) 308, 1022, 1126, 1364, 1386
BseGI GGATG 8 cut(s) 431, 444, 796, 1062, 1090, 1300, 1342, 1375
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 3 cut(s) 302, 981, 1392
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 5 cut(s) 198, 802, 1046, 1183, 1204
BseRI GAGGAG 1 cut(s) 1426
BseXI GCAGC 3 cut(s) 673, 842, 890
BseYI CCCAGC 2 cut(s) 881, 1066
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 396, 1125, 1396
BsiHKAI GWGCWC 2 cut(s) 1249, 1405
BsiSI CCGG 3 cut(s) 286, 1242, 1408
BslFI GGGAC 2 cut(s) 909, 1354
BslI CCNNNNNNNGG 3 cut(s) 302, 981, 1392
BsmAI GTCTC 3 cut(s) 788, 1252, 1281
BsmFI GGGAC 2 cut(s) 909, 1354
BsnI GGCC 3 cut(s) 396, 1125, 1396
Bsp1286I GDGCHC 2 cut(s) 1249, 1405
Bsp1407I TGTACA 1 cut(s) 1014
Bsp19I CCATGG 1 cut(s) 1126
BspACI CCGC 2 cut(s) 246, 788
BspANI GGCC 3 cut(s) 396, 1125, 1396
BspCNI CTCAG 1 cut(s) 662
BspFNI CGCG 1 cut(s) 246
BspHI TCATGA 3 cut(s) 112, 595, 1036
BspLI GGNNCC 2 cut(s) 85, 887
BspOI GCTAGC 1 cut(s) 522
BspPI GGATC 2 cut(s) 562, 1210
BspQI GCTCTTC 1 cut(s) 1333
BsrFI RCCGGY 1 cut(s) 285
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 5 cut(s) 198, 802, 1046, 1183, 1204
BssAI RCCGGY 1 cut(s) 285
BssECI CCNNGG 5 cut(s) 308, 1022, 1126, 1364, 1386
BssT1I CCWWGG 3 cut(s) 308, 1022, 1126
Bst2UI CCWGG 4 cut(s) 167, 177, 1365, 1388
Bst4CI ACNGT 4 cut(s) 269, 535, 778, 1292
Bst6I CTCTTC 1 cut(s) 1333
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 2 cut(s) 520, 1405
BstDEI CTNAG 3 cut(s) 132, 649, 1028
BstDSI CCRYGG 1 cut(s) 1126
BstEII GGTNACC 1 cut(s) 1237
BstF5I GGATG 8 cut(s) 431, 444, 796, 1062, 1090, 1300, 1342, 1375
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 1 cut(s) 38
BstMAI GTCTC 3 cut(s) 788, 1252, 1281
BstMWI GCNNNNNNNGC 1 cut(s) 1253
BstNI CCWGG 4 cut(s) 167, 177, 1365, 1388
BstNSI RCATGY 1 cut(s) 175
BstPI GGTNACC 1 cut(s) 1237
BstSCI CCNGG 4 cut(s) 165, 175, 1363, 1386
BstSFI CTRYAG 1 cut(s) 1291
BstUI CGCG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 673, 842, 890
BstX2I RGATCY 2 cut(s) 554, 1202
BstYI RGATCY 2 cut(s) 554, 1202
BsuRI GGCC 3 cut(s) 396, 1125, 1396
BtgI CCRYGG 1 cut(s) 1126
BtsCI GGATG 8 cut(s) 431, 444, 796, 1062, 1090, 1300, 1342, 1375
BtsI GCAGTG 1 cut(s) 826
BtsIMutI CAGTG 4 cut(s) 531, 774, 826, 1197
Cac8I GCNNGC 2 cut(s) 520, 1405
CciI TCATGA 3 cut(s) 112, 595, 1036
CfoI GCGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 285
Cfr13I GGNCC 5 cut(s) 83, 305, 886, 964, 1277
CseI GACGC 2 cut(s) 252, 861
Csp6I GTAC 6 cut(s) 1015, 1063, 1140, 1176, 1189, 1379
CviAII CATG 9 cut(s) 113, 172, 275, 523, 596, 1012, 1037, 1073, 1127
CviQI GTAC 6 cut(s) 1015, 1063, 1140, 1176, 1189, 1379
DdeI CTNAG 3 cut(s) 132, 649, 1028
Eam1104I CTCTTC 1 cut(s) 1333
EarI CTCTTC 1 cut(s) 1333
Ecl136II GAGCTC 1 cut(s) 1247
Eco130I CCWWGG 3 cut(s) 308, 1022, 1126
Eco24I GRGCYC 1 cut(s) 1249
Eco47I GGWCC 5 cut(s) 83, 305, 886, 964, 1277
Eco47III AGCGCT 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 1247
Eco91I GGTNACC 1 cut(s) 1237
EcoICRI GAGCTC 1 cut(s) 1247
EcoO109I RGGNCCY 3 cut(s) 83, 886, 1277
EcoO65I GGTNACC 1 cut(s) 1237
EcoRII CCWGG 4 cut(s) 165, 175, 1363, 1386
EcoT14I CCWWGG 3 cut(s) 308, 1022, 1126
EcoT38I GRGCYC 1 cut(s) 1249
ErhI CCWWGG 3 cut(s) 308, 1022, 1126
FaeI CATG 9 cut(s) 116, 175, 278, 526, 599, 1015, 1040, 1076, 1130
FalI AAGNNNNNCTT 2 cut(s) 322, 354
FaqI GGGAC 2 cut(s) 909, 1354
FatI CATG 9 cut(s) 112, 171, 274, 522, 595, 1011, 1036, 1072, 1126
FauI CCCGC 1 cut(s) 239
FbaI TGATCA 3 cut(s) 109, 1099, 1210
Fnu4HI GCNGC 3 cut(s) 687, 856, 879
FokI GGATG 8 cut(s) 431, 438, 803, 1069, 1077, 1287, 1349, 1382
FriOI GRGCYC 1 cut(s) 1249
Fsp4HI GCNGC 3 cut(s) 687, 856, 879
FspBI CTAG 3 cut(s) 519, 1005, 1206
GlaI GCGC 1 cut(s) 37
GluI GCNGC 3 cut(s) 687, 856, 879
GsaI CCCAGC 2 cut(s) 885, 1070
GsuI CTGGAG 2 cut(s) 1149, 1409
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 3 cut(s) 396, 1125, 1396
HapII CCGG 3 cut(s) 286, 1242, 1408
HgaI GACGC 2 cut(s) 252, 861
HhaI GCGC 1 cut(s) 38
Hin1II CATG 9 cut(s) 116, 175, 278, 526, 599, 1015, 1040, 1076, 1130
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HincII GTYRAC 2 cut(s) 327, 739
HindII GTYRAC 2 cut(s) 327, 739
HindIII AAGCTT 1 cut(s) 457
HinfI GANTC 5 cut(s) 252, 323, 569, 1131, 1260
HpaI GTTAAC 1 cut(s) 739
HpaII CCGG 3 cut(s) 286, 1242, 1408
HphI GGTGA 2 cut(s) 635, 1249
Hpy166II GTNNAC 4 cut(s) 319, 327, 739, 772
Hpy188I TCNGA 4 cut(s) 438, 746, 766, 1314
Hpy8I GTNNAC 4 cut(s) 319, 327, 739, 772
HpyAV CCTTC 7 cut(s) 56, 433, 593, 899, 955, 1145, 1268
HpyCH4III ACNGT 4 cut(s) 269, 535, 778, 1292
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 3 cut(s) 578, 689, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 1253
HpyF3I CTNAG 3 cut(s) 132, 649, 1028
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 9 cut(s) 116, 175, 278, 526, 599, 1015, 1040, 1076, 1130
HspAI GCGC 1 cut(s) 36
Ksp22I TGATCA 3 cut(s) 109, 1099, 1210
KspAI GTTAAC 1 cut(s) 739
LguI GCTCTTC 1 cut(s) 1333
LmnI GCTCC 1 cut(s) 1244
Lsp1109I GCAGC 3 cut(s) 673, 842, 890
LweI GCATC 2 cut(s) 688, 1309
MaeI CTAG 3 cut(s) 519, 1005, 1206
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 9 cut(s) 5, 189, 570, 583, 821, 910, 941, 1154, 1237
MboII GAAGA 2 cut(s) 549, 1320
MflI RGATCY 2 cut(s) 554, 1202
MhlI GDGCHC 2 cut(s) 1249, 1405
MluCI AATT 8 cut(s) 117, 350, 471, 591, 635, 642, 1146, 1358
MlyI GAGTC 4 cut(s) 332, 578, 1140, 1254
MmeI TCCRAC 1 cut(s) 532
MseI TTAA 5 cut(s) 402, 738, 860, 947, 1145
MslI CAYNNNNRTG 1 cut(s) 1095
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 3 cut(s) 286, 1242, 1408
MspR9I CCNGG 4 cut(s) 167, 177, 1365, 1388
MvaI CCWGG 4 cut(s) 167, 177, 1365, 1388
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 1 cut(s) 1253
NcoI CCATGG 1 cut(s) 1126
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 9 cut(s) 116, 175, 278, 526, 599, 1015, 1040, 1076, 1130
NlaIV GGNNCC 2 cut(s) 85, 887
NmeAIII GCCGAG 1 cut(s) 1422
NmuCI GTSAC 3 cut(s) 570, 821, 1237
NspI RCATGY 1 cut(s) 175
PagI TCATGA 3 cut(s) 112, 595, 1036
PciSI GCTCTTC 1 cut(s) 1333
PfeI GAWTC 1 cut(s) 252
PflFI GACNNNGTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 981
PkrI GCNGC 3 cut(s) 688, 857, 880
PleI GAGTC 4 cut(s) 331, 577, 1139, 1254
PpsI GAGTC 4 cut(s) 331, 577, 1139, 1254
PpuMI RGGWCCY 3 cut(s) 83, 886, 1277
Psp124BI GAGCTC 1 cut(s) 1249
Psp1406I AACGTT 1 cut(s) 502
Psp5II RGGWCCY 3 cut(s) 83, 886, 1277
Psp6I CCWGG 4 cut(s) 165, 175, 1363, 1386
PspEI GGTNACC 1 cut(s) 1237
PspFI CCCAGC 2 cut(s) 881, 1066
PspGI CCWGG 4 cut(s) 165, 175, 1363, 1386
PspN4I GGNNCC 2 cut(s) 85, 887
PspPI GGNCC 5 cut(s) 83, 305, 886, 964, 1277
PspPPI RGGWCCY 3 cut(s) 83, 886, 1277
PsuI RGATCY 2 cut(s) 554, 1202
PsyI GACNNNGTC 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 881
RsaI GTAC 6 cut(s) 1016, 1064, 1141, 1177, 1190, 1380
RsaNI GTAC 6 cut(s) 1015, 1063, 1140, 1176, 1189, 1379
RseI CAYNNNNRTG 1 cut(s) 1095
SacI GAGCTC 1 cut(s) 1249
SapI GCTCTTC 1 cut(s) 1333
SaqAI TTAA 5 cut(s) 402, 738, 860, 947, 1145
SatI GCNGC 3 cut(s) 687, 856, 879
Sau96I GGNCC 5 cut(s) 83, 305, 886, 964, 1277
ScaI AGTACT 1 cut(s) 1177
SchI GAGTC 4 cut(s) 332, 578, 1140, 1254
ScrFI CCNGG 4 cut(s) 167, 177, 1365, 1388
SduI GDGCHC 2 cut(s) 1249, 1405
SfaNI GCATC 2 cut(s) 688, 1309
SfcI CTRYAG 1 cut(s) 1291
SinI GGWCC 5 cut(s) 83, 305, 886, 964, 1277
SmiMI CAYNNNNRTG 1 cut(s) 1095
SmlI CTYRAG 2 cut(s) 460, 1248
SmoI CTYRAG 2 cut(s) 460, 1248
Sse9I AATT 8 cut(s) 117, 350, 471, 591, 635, 642, 1146, 1358
SsiI CCGC 2 cut(s) 246, 788
SspI AATATT 1 cut(s) 541
SspMI CTAG 3 cut(s) 519, 1005, 1206
SstI GAGCTC 1 cut(s) 1249
StyD4I CCNGG 4 cut(s) 165, 175, 1363, 1386
StyI CCWWGG 3 cut(s) 308, 1022, 1126
TaaI ACNGT 4 cut(s) 269, 535, 778, 1292
TaiI ACGT 1 cut(s) 505
TasI AATT 8 cut(s) 117, 350, 471, 591, 635, 642, 1146, 1358
TatI WGTACW 3 cut(s) 1014, 1175, 1188
TfiI GAWTC 1 cut(s) 252
Tru1I TTAA 5 cut(s) 402, 738, 860, 947, 1145
Tru9I TTAA 5 cut(s) 402, 738, 860, 947, 1145
TscAI CASTG 4 cut(s) 538, 781, 826, 1204
TseFI GTSAC 3 cut(s) 570, 821, 1237
TseI GCWGC 3 cut(s) 686, 855, 878
Tsp45I GTSAC 3 cut(s) 570, 821, 1237
TspDTI ATGAA 5 cut(s) 105, 129, 221, 584, 612
TspGWI ACGGA 1 cut(s) 602
TspRI CASTG 4 cut(s) 538, 781, 826, 1204
Tth111I GACNNNGTC 1 cut(s) 568
Van91I CCANNNNNTGG 1 cut(s) 981
VpaK11BI GGWCC 5 cut(s) 83, 305, 886, 964, 1277
XapI RAATTY 4 cut(s) 591, 635, 642, 1358
XbaI TCTAGA 1 cut(s) 1004
XceI RCATGY 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XspI CTAG 3 cut(s) 519, 1005, 1206
ZrmI AGTACT 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.