Rmu_sc0017106.1_g000005

LEC14B protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017106.1
OG0001751
Physical Location & Seq
Reverse (-)
14230 .. 17458
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017106.1_g000005.1.cds

Sequence Viewer

Length: 1497 bp
atgtttgtaacatcaattgatgattttgatgagatggggtattcactcagtagattgactgtagaatctgactattttgatgatgggagtaccaacaacggatcagttgagagcattgatggacccaacaaacagtttgatagtttagaccatgaagtagctcagctcacaaagctgagatcagcacccaatgagaggcttaggcaagtcggaccggggaggcggggccgacctgtttcgacagtgaagatgctggaagggagagaaggtaattattcaggaagggggaggttctcttctgcagattgttgtcatgtttcaggtagatacttgccgctgaatggtccttggctcgttgaccagatgcctagccgagcatatgtctcgcagttttcagcagatggatctctttttgttgcggggtttcagggtagccacattagaatatacaatgtggataaagggtggaaagtccagaaggacattcatgccaagagtatgagatggacagtgactgatacatctctttccccagatcaacgccatcttgtctatgcgagcatgtcacctattgttcatatagttaatgtgggatctgctgaaacagaatctcgtgctaatataatggaaatccatgagggtttggatttttcttctgctgatgatggagtatactcctttggtattttctccataaaattttcaacagatggacgtgaagttgttgctggaagtagtgatgggtccatatatgtttatgatcttgaaactaataagctctcccttcgaattttggctcacacgtctgatgttaacactgtttgttttgccgatgaaagtggccatctcatttattctgggagtgatgatactctctgtaaggtatgggatagacgctgctttaaagctagtgggaagccagcaggagttttgacagggcacctagaaggtattacattcattgacagcaaaggagatggtcgttacttcatttcaaatagcaaagatcagagcataaaactttgggatatcaggaaaatgtcctctagtgctactgctgcccgcaatccagggcctaggtatcatgaatgggattacagatggatggactacccacctcaggcaaaagatatgaaacatccatctgatatatcagtgtctacttataaaggtcattcggtcttgcgtacacttattcgctcctacttctccccggcgtatagcactggtcagaagtacatctacactggatctcacaattcatgcgtttatatatatgatttggtcaatggagcccaagttggaaaattggagcatcataaatcagctgtaagagattgtagttggcacccatattaccctatgctcatcagctcttcctgggatggggaggttgttagatgggaattcacaggaaatggagaatcaccaccacctttcaccagcaagagagcctggaggagaccatattcaccaacaccagttatgatattatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

498

Amino Acids

55.79

Weight (kDa)

6.63

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
AccB1I GGYRCC 2 cut(s) 939, 1347
AccI GTMKAC 2 cut(s) 672, 1160
AciI CCGC 4 cut(s) 223, 335, 419, 1063
AclWI GGATC 4 cut(s) 109, 412, 601, 1258
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 3 cut(s) 698, 789, 1406
AfaI GTAC 3 cut(s) 91, 1189, 1238
AfiI CCNNNNNNNGG 4 cut(s) 195, 341, 1120, 1386
AflIII ACRYGT 1 cut(s) 801
AgsI TTSAA 3 cut(s) 705, 767, 996
AjiI CACGTC 2 cut(s) 716, 804
AjnI CCWGG 3 cut(s) 1069, 1379, 1454
AluBI AGCT 7 cut(s) 161, 166, 175, 778, 908, 1328, 1374
AluI AGCT 7 cut(s) 161, 166, 175, 778, 908, 1328, 1374
Alw26I GTCTC 2 cut(s) 388, 1456
AlwI GGATC 4 cut(s) 109, 412, 601, 1258
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 3 cut(s) 226, 841, 1073
ApeKI GCWGC 2 cut(s) 897, 1058
ApoI RAATTY 3 cut(s) 698, 789, 1406
AspA2I CCTAGG 1 cut(s) 1076
AspS9I GGNCC 6 cut(s) 122, 212, 226, 344, 744, 1073
AsuC2I CCSGG 2 cut(s) 216, 1214
AsuHPI GGTGA 4 cut(s) 558, 1419, 1432, 1464
AsuII TTCGAA 1 cut(s) 787
AvaII GGWCC 4 cut(s) 122, 212, 344, 744
AvrII CCTAGG 1 cut(s) 1076
AxyI CCTNAGG 1 cut(s) 1119
BaeGI GKGCMC 1 cut(s) 942
BalI TGGCCA 1 cut(s) 843
BanI GGYRCC 2 cut(s) 939, 1347
BanII GRGCYC 1 cut(s) 1297
BarI GAAGNNNNNNTAC 2 cut(s) 1226, 1258
BauI CACGAG 1 cut(s) 612
BbvI GCAGC 2 cut(s) 884, 1045
BcgI CGANNNNNNTGC 2 cut(s) 366, 400
BciT130I CCWGG 3 cut(s) 1071, 1381, 1456
BcnI CCSGG 2 cut(s) 216, 1214
BcoDI GTCTC 2 cut(s) 388, 1456
BfaI CTAG 5 cut(s) 369, 909, 944, 1047, 1077
BfmI CTRYAG 2 cut(s) 60, 300
BisI GCNGC 3 cut(s) 335, 898, 1059
BlnI CCTAGG 1 cut(s) 1076
BlpI GCTNAGC 1 cut(s) 162
BlsI GCNGC 3 cut(s) 336, 899, 1060
Bme1390I CCNGG 5 cut(s) 216, 1071, 1214, 1381, 1456
Bme18I GGWCC 4 cut(s) 122, 212, 344, 744
BmgBI CACGTC 2 cut(s) 716, 804
BmgT120I GGNCC 6 cut(s) 122, 212, 226, 344, 744, 1073
BmiI GGNNCC 6 cut(s) 124, 227, 745, 941, 1294, 1349
BmrFI CCNGG 5 cut(s) 216, 1071, 1214, 1381, 1456
BmsI GCATC 3 cut(s) 240, 354, 1324
BpmI CTGGAG 1 cut(s) 1477
Bpu10I CCTNAGC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 162
Bpu14I TTCGAA 1 cut(s) 787
BpuMI CCSGG 2 cut(s) 216, 1214
BsaI GGTCTC 1 cut(s) 1456
BsaJI CCNNGG 6 cut(s) 215, 347, 1070, 1076, 1212, 1380
BsaXI ACNNNNNCTCC 4 cut(s) 253, 283, 1284, 1314
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 341, 1120, 1386
Bse1I ACTGG 3 cut(s) 1231, 1252, 1481
Bse21I CCTNAGG 1 cut(s) 1119
BseBI CCWGG 3 cut(s) 1071, 1381, 1456
BseDI CCNNGG 6 cut(s) 215, 347, 1070, 1076, 1212, 1380
BseGI GGATG 3 cut(s) 1110, 1138, 1390
BseLI CCNNNNNNNGG 4 cut(s) 195, 341, 1120, 1386
BseMII CTCAG 4 cut(s) 61, 167, 176, 1133
BseNI ACTGG 3 cut(s) 1231, 1252, 1481
BseRI GAGGAG 1 cut(s) 1474
BseSI GKGCMC 1 cut(s) 942
BseXI GCAGC 2 cut(s) 884, 1045
BshFI GGCC 3 cut(s) 228, 843, 1075
BshNI GGYRCC 2 cut(s) 939, 1347
BsiSI CCGG 2 cut(s) 215, 1214
BslI CCNNNNNNNGG 4 cut(s) 195, 341, 1120, 1386
BsmAI GTCTC 2 cut(s) 388, 1456
BsnI GGCC 3 cut(s) 228, 843, 1075
Bso31I GGTCTC 1 cut(s) 1456
Bsp119I TTCGAA 1 cut(s) 787
Bsp1286I GDGCHC 2 cut(s) 942, 1297
Bsp143I GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
Bsp1720I GCTNAGC 1 cut(s) 162
BspACI CCGC 4 cut(s) 223, 335, 419, 1063
BspANI GGCC 3 cut(s) 228, 843, 1075
BspCNI CTCAG 4 cut(s) 60, 168, 175, 1132
BspHI TCATGA 1 cut(s) 1084
BspLI GGNNCC 6 cut(s) 124, 227, 745, 941, 1294, 1349
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 4 cut(s) 109, 412, 601, 1258
BspQI GCTCTTC 1 cut(s) 1381
BspT104I TTCGAA 1 cut(s) 787
BspT107I GGYRCC 2 cut(s) 939, 1347
BspTNI GGTCTC 1 cut(s) 1456
BsrI ACTGG 3 cut(s) 1231, 1252, 1481
BssECI CCNNGG 6 cut(s) 215, 347, 1070, 1076, 1212, 1380
BssMI GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
BssNAI GTATAC 1 cut(s) 673
BssSI CACGAG 1 cut(s) 612
BssT1I CCWWGG 2 cut(s) 347, 1076
Bst1107I GTATAC 1 cut(s) 673
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 3 cut(s) 1071, 1381, 1456
Bst4CI ACNGT 5 cut(s) 61, 135, 244, 511, 820
Bst6I CTCTTC 2 cut(s) 301, 1381
BstBI TTCGAA 1 cut(s) 787
BstC8I GCNNGC 3 cut(s) 559, 921, 1063
BstDEI CTNAG 5 cut(s) 47, 162, 176, 200, 1119
BstF5I GGATG 3 cut(s) 1110, 1138, 1390
BstKTI GATC 8 cut(s) 104, 182, 407, 538, 596, 763, 1009, 1253
BstMAI GTCTC 2 cut(s) 388, 1456
BstMBI GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
BstMWI GCNNNNNNNGC 2 cut(s) 172, 1058
BstNI CCWGG 3 cut(s) 1071, 1381, 1456
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 5 cut(s) 214, 1069, 1212, 1379, 1454
BstSFI CTRYAG 2 cut(s) 60, 300
BstSLI GKGCMC 1 cut(s) 942
BstV1I GCAGC 2 cut(s) 884, 1045
BstX2I RGATCY 3 cut(s) 404, 593, 1250
BstYI RGATCY 3 cut(s) 404, 593, 1250
BstZ17I GTATAC 1 cut(s) 673
Bsu36I CCTNAGG 1 cut(s) 1119
BsuRI GGCC 3 cut(s) 228, 843, 1075
BtrI CACGTC 2 cut(s) 716, 804
BtsCI GGATG 3 cut(s) 1110, 1138, 1390
BtsIMutI CAGTG 6 cut(s) 249, 516, 816, 1161, 1224, 1245
Cac8I GCNNGC 3 cut(s) 559, 921, 1063
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 1084
Cfr13I GGNCC 6 cut(s) 122, 212, 226, 344, 744, 1073
CpoI CGGWCCG 1 cut(s) 212
CseI GACGC 1 cut(s) 903
Csp6I GTAC 3 cut(s) 90, 1188, 1237
CspI CGGWCCG 1 cut(s) 212
CviAII CATG 7 cut(s) 152, 314, 488, 562, 635, 1085, 1263
CviQI GTAC 3 cut(s) 90, 1188, 1237
DdeI CTNAG 5 cut(s) 47, 162, 176, 200, 1119
DpnI GATC 8 cut(s) 103, 181, 406, 537, 595, 762, 1008, 1252
DpnII GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
DraI TTTAAA 1 cut(s) 904
EaeI YGGCCR 1 cut(s) 841
Eam1104I CTCTTC 2 cut(s) 301, 1381
EarI CTCTTC 2 cut(s) 301, 1381
Eco130I CCWWGG 2 cut(s) 347, 1076
Eco24I GRGCYC 1 cut(s) 1297
Eco31I GGTCTC 1 cut(s) 1456
Eco32I GATATC 1 cut(s) 1030
Eco47I GGWCC 4 cut(s) 122, 212, 344, 744
Eco81I CCTNAGG 1 cut(s) 1119
EcoO109I RGGNCCY 1 cut(s) 1073
EcoRI GAATTC 1 cut(s) 1406
EcoRII CCWGG 3 cut(s) 1069, 1379, 1454
EcoRV GATATC 1 cut(s) 1030
EcoT14I CCWWGG 2 cut(s) 347, 1076
EcoT38I GRGCYC 1 cut(s) 1297
ErhI CCWWGG 2 cut(s) 347, 1076
FaeI CATG 7 cut(s) 155, 317, 491, 565, 638, 1088, 1266
FatI CATG 7 cut(s) 151, 313, 487, 561, 634, 1084, 1262
FauI CCCGC 3 cut(s) 216, 412, 1070
FauNDI CATATG 1 cut(s) 379
FblI GTMKAC 2 cut(s) 672, 1160
Fnu4HI GCNGC 3 cut(s) 335, 898, 1059
FokI GGATG 3 cut(s) 1117, 1125, 1397
FriOI GRGCYC 1 cut(s) 1297
Fsp4HI GCNGC 3 cut(s) 335, 898, 1059
FspBI CTAG 5 cut(s) 369, 909, 944, 1047, 1077
GluI GCNGC 3 cut(s) 335, 898, 1059
GsuI CTGGAG 1 cut(s) 1477
HaeIII GGCC 3 cut(s) 228, 843, 1075
HapII CCGG 2 cut(s) 215, 1214
HgaI GACGC 1 cut(s) 903
Hin1II CATG 7 cut(s) 155, 317, 491, 565, 638, 1088, 1266
HincII GTYRAC 2 cut(s) 358, 814
HindII GTYRAC 2 cut(s) 358, 814
HinfI GANTC 3 cut(s) 65, 608, 1424
HpaI GTTAAC 1 cut(s) 814
HpaII CCGG 2 cut(s) 215, 1214
HphI GGTGA 4 cut(s) 558, 1419, 1432, 1464
Hpy166II GTNNAC 5 cut(s) 358, 673, 814, 1161, 1190
Hpy188I TCNGA 6 cut(s) 70, 212, 808, 1011, 1147, 1233
Hpy188III TCNNGA 5 cut(s) 279, 475, 764, 1033, 1085
Hpy8I GTNNAC 5 cut(s) 358, 673, 814, 1161, 1190
HpyAV CCTTC 6 cut(s) 251, 260, 276, 472, 794, 941
HpyCH4III ACNGT 5 cut(s) 61, 135, 244, 511, 820
HpyCH4IV ACGT 2 cut(s) 715, 803
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 1058
HpyF3I CTNAG 5 cut(s) 47, 162, 176, 200, 1119
HpySE526I ACGT 2 cut(s) 715, 803
Hsp92II CATG 7 cut(s) 155, 317, 491, 565, 638, 1088, 1266
KspAI GTTAAC 1 cut(s) 814
Kzo9I GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
LguI GCTCTTC 1 cut(s) 1381
LmnI GCTCC 3 cut(s) 1205, 1292, 1312
Lsp1109I GCAGC 2 cut(s) 884, 1045
LweI GCATC 3 cut(s) 240, 354, 1324
MaeI CTAG 5 cut(s) 369, 909, 944, 1047, 1077
MaeII ACGT 2 cut(s) 715, 803
MaeIII GTNAC 4 cut(s) 7, 511, 564, 983
MalI GATC 8 cut(s) 103, 181, 406, 537, 595, 762, 1008, 1252
MboI GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
MboII GAAGA 4 cut(s) 259, 288, 645, 1368
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 3 cut(s) 404, 593, 1250
MhlI GDGCHC 2 cut(s) 942, 1297
MlsI TGGCCA 1 cut(s) 843
MluCI AATT 7 cut(s) 15, 271, 698, 789, 1258, 1307, 1406
MluNI TGGCCA 1 cut(s) 843
MmeI TCCRAC 2 cut(s) 190, 1282
MnlI CCTC 8 cut(s) 189, 213, 282, 631, 1054, 1128, 1384, 1452
Mox20I TGGCCA 1 cut(s) 843
MscI TGGCCA 1 cut(s) 843
MseI TTAA 3 cut(s) 585, 813, 903
Msp20I TGGCCA 1 cut(s) 843
MspA1I CMGCKG 2 cut(s) 337, 1328
MspI CCGG 2 cut(s) 215, 1214
MspR9I CCNGG 5 cut(s) 216, 1071, 1214, 1381, 1456
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 3 cut(s) 1071, 1381, 1456
MwoI GCNNNNNNNGC 2 cut(s) 172, 1058
NciI CCSGG 2 cut(s) 216, 1214
NdeI CATATG 1 cut(s) 379
NdeII GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
NlaIII CATG 7 cut(s) 155, 317, 491, 565, 638, 1088, 1266
NlaIV GGNNCC 6 cut(s) 124, 227, 745, 941, 1294, 1349
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 2 cut(s) 511, 564
NspI RCATGY 1 cut(s) 565
NspV TTCGAA 1 cut(s) 787
PagI TCATGA 1 cut(s) 1084
PciSI GCTCTTC 1 cut(s) 1381
PfeI GAWTC 3 cut(s) 65, 608, 1424
PkrI GCNGC 3 cut(s) 336, 899, 1060
PsiI TTATAA 1 cut(s) 1167
Psp6I CCWGG 3 cut(s) 1069, 1379, 1454
PspGI CCWGG 3 cut(s) 1069, 1379, 1454
PspN4I GGNNCC 6 cut(s) 124, 227, 745, 941, 1294, 1349
PspPI GGNCC 6 cut(s) 122, 212, 226, 344, 744, 1073
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 3 cut(s) 404, 593, 1250
PvuII CAGCTG 1 cut(s) 1328
RsaI GTAC 3 cut(s) 91, 1189, 1238
RsaNI GTAC 3 cut(s) 90, 1188, 1237
Rsr2I CGGWCCG 1 cut(s) 212
RsrII CGGWCCG 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 1381
SaqAI TTAA 3 cut(s) 585, 813, 903
SatI GCNGC 3 cut(s) 335, 898, 1059
Sau3AI GATC 8 cut(s) 101, 179, 404, 535, 593, 760, 1006, 1250
Sau96I GGNCC 6 cut(s) 122, 212, 226, 344, 744, 1073
ScrFI CCNGG 5 cut(s) 216, 1071, 1214, 1381, 1456
SduI GDGCHC 2 cut(s) 942, 1297
SfaNI GCATC 3 cut(s) 240, 354, 1324
SfcI CTRYAG 2 cut(s) 60, 300
SfuI TTCGAA 1 cut(s) 787
SinI GGWCC 4 cut(s) 122, 212, 344, 744
Sse9I AATT 7 cut(s) 15, 271, 698, 789, 1258, 1307, 1406
SsiI CCGC 4 cut(s) 223, 335, 419, 1063
SspMI CTAG 5 cut(s) 369, 909, 944, 1047, 1077
StyD4I CCNGG 5 cut(s) 214, 1069, 1212, 1379, 1454
StyI CCWWGG 2 cut(s) 347, 1076
TaaI ACNGT 5 cut(s) 61, 135, 244, 511, 820
TaiI ACGT 2 cut(s) 718, 806
TaqI TCGA 2 cut(s) 239, 787
TaqII GACCGA 1 cut(s) 1168
TasI AATT 7 cut(s) 15, 271, 698, 789, 1258, 1307, 1406
TatI WGTACW 1 cut(s) 1236
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 3 cut(s) 65, 608, 1424
Tru1I TTAA 3 cut(s) 585, 813, 903
Tru9I TTAA 3 cut(s) 585, 813, 903
TscAI CASTG 6 cut(s) 249, 516, 823, 1161, 1231, 1252
TseFI GTSAC 2 cut(s) 511, 564
TseI GCWGC 2 cut(s) 897, 1058
Tsp45I GTSAC 2 cut(s) 511, 564
TspDTI ATGAA 9 cut(s) 168, 476, 566, 849, 949, 979, 1101, 1148, 1251
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 6 cut(s) 249, 516, 823, 1161, 1231, 1252
VpaK11BI GGWCC 4 cut(s) 122, 212, 344, 744
XapI RAATTY 3 cut(s) 698, 789, 1406
XceI RCATGY 1 cut(s) 565
XmaJI CCTAGG 1 cut(s) 1076
XmiI GTMKAC 2 cut(s) 672, 1160
XspI CTAG 5 cut(s) 369, 909, 944, 1047, 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.