RLG00000007761

LEC14B homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001751
Physical Location & Seq
Forward (+)
20727384 .. 20732429
5046 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007761

Sequence Viewer

Length: 1422 bp
ATGAGTTACAGAAGGAGGTTTGCGAAAGATAATAGTGCCAGTGATAGTGGAAATGCTGTTGAAGGTTCTGCTTCAAGTGAAGAAGCCAATGAAGCATCAAAGAGTCTTGATCATGAAGTGGCTCACCTCACCAAGCTTAGATCAAGACCCCATCAGCATTTGAGGGGGGTCTTGCCCGGAAAGTTGAGGTTACCGGTGTCAACACTGAAAATGTTGATGGGCCGTGAAAGTAATTATTCGGGGCGAGGGAGGTTTTCGTCAGCAGATGCTTGTCATGTTTTAAGCCGGTATCTGCCCATTAATGGTCCATGGGGGGTGGACCAGTTGAATAGCCCTGCATATGTTTCTCAGTTTTCGAATGATGGATTGTTTTTTGTTGCTGGATTTCAGGGAGGTCATATTAGAATATATAATGTTGAAAAGGGATGGACAGTTCAGAAGGACATTCTAACCAGAAGCTTGAGATGGACAATTACTGATACATCTCTATCACCAGATCAACGGTTTCTTGCATATGCTAGCATGTCACCTATTGTCCATATTGTCAATGCTGGATCTTCTACGACAGAGTCACTTGCAAACATTACGGAAATACATGAAGATCTGGATTTTTCTGTTGATTATGATGAAGAATTTGGAATATTCTCTGTTAAATTTTCAACTGATGGGCGAGAGCTTGTGGCTGGAAGTAATGATGCTTCAATACATGTTTATGATCTCCAAGCAAACAAAGTTAGCCTCAAAATACCAGCGCATACGTCAGATGTAAACACAGTATGCTTTGCAGATGAGACTGGCCATCTCATATTTTCCGGCAGTGATGATAACCTCTGTAAGGTGTGGGATAGACGCTGCTTTATCACGAAAGGGCAAGCAGCTGGGGTTCTGATGGGACATCTTGAAGGCATAACGTTTATTGATAGTCGGGGAGATGGACGTTACTTAATATCAAATGGGAAGGATCAGACTACCAAACTTTGGGATATACGGAAGATGTCCTCTAGAAATAAGTATCGAAGGAATACAGATTATGACTGGGATTACAGGTGGATGGAGTACCCAGCTCATGCAAGAACTTTGAGACATCCCTTGGATCAGTCGTTGGCTACATATAGAGACCATTCAGTCCTGCGCACTTTAATTCGCTGTTACTTTTCTCCAGCATATAGCACTGGACAAAAGTACATCTACACTGGATCTAGTGATCATTCGGTCTATATTTATGATCTGGTGACTGGCGCTCAAGTTGCGAAACTCTTCCATCATGATGGACCTGTAAGAGATTGTAGTTGGCATCCTACATATCCCATGTTGGTCAGTTCTTCCTGGGATGGGAGTATCGTGAGATGGGAGTTTCCGGGAGATGATCAAGTTCCTACCCTGGTCAAGCCAAGGGTAAGGCGGAGGAGAGTTTTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

474

Amino Acids

53.54

Weight (kDa)

8.11

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML_2 PF23414 108 - 207 2.8e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 117 - 338 4.6e-22 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 124 - 331 5.9e-10 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 127 - 281 8.1e-09 WDR36/Utp21 first beta-propeller
WDR55 PF24796 157 - 333 7.8e-16 WDR55
WD40_Prp19 PF24814 159 - 284 3.3e-11 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 178 - 341 2.1e-10 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 180 - 284 2.6e-09 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 205 - 340 1.9e-16 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 208 - 328 3.6e-13 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 212 - 336 1.2e-15 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 213 - 337 2.5e-19 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 214 - 282 4.6e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 214 - 347 1.6e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 214 - 341 7.1e-19 THOC3 beta-propeller domain
WD40 PF00400 248 - 282 2.2e-06 WD domain, G-beta repeat
EIF3I PF24805 258 - 445 8.7e-10 EIF3I
WD40_WDHD1_1st PF24817 297 - 460 1.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 380 - 450 2.1e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 388 - 464 4.7e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 388 - 453 1.1e-06 THOC3 beta-propeller domain
WD40_Prp19 PF24814 392 - 456 4.6e-08 Prp19 WD40 domain
WD40 PF00400 415 - 450 1.2e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1124
Acc16I TGCGCA 1 cut(s) 1133
AccB7I CCANNNNNTGG 1 cut(s) 978
AciI CCGC 1 cut(s) 1402
AclI AACGTT 1 cut(s) 911
AclWI GGATC 4 cut(s) 562, 969, 1101, 1204
AcoI YGGCCR 1 cut(s) 796
AcsI RAATTY 2 cut(s) 632, 653
AfaI GTAC 2 cut(s) 1058, 1184
AfiI CCNNNNNNNGG 4 cut(s) 302, 835, 978, 1332
AflIII ACRYGT 1 cut(s) 706
AgeI ACCGGT 1 cut(s) 193
AgsI TTSAA 7 cut(s) 62, 75, 328, 419, 660, 702, 902
AjnI CCWGG 2 cut(s) 1325, 1380
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 5 cut(s) 136, 459, 676, 878, 1064
AluI AGCT 5 cut(s) 136, 459, 676, 878, 1064
Alw26I GTCTC 3 cut(s) 785, 1075, 1110
AlwI GGATC 4 cut(s) 562, 969, 1101, 1204
AoxI GGCC 2 cut(s) 220, 796
ApeKI GCWGC 2 cut(s) 852, 875
ApoI RAATTY 2 cut(s) 632, 653
AseI ATTAAT 1 cut(s) 300
AsiGI ACCGGT 1 cut(s) 193
Asp700I GAANNNNTTC 1 cut(s) 1256
AspLEI GCGC 3 cut(s) 754, 1134, 1241
AspS9I GGNCC 4 cut(s) 220, 305, 319, 1271
AsuC2I CCSGG 2 cut(s) 177, 1359
AsuHPI GGTGA 5 cut(s) 116, 121, 483, 519, 1243
AsuII TTCGAA 1 cut(s) 356
AsuNHI GCTAGC 1 cut(s) 518
AvaII GGWCC 3 cut(s) 305, 319, 1271
BalI TGGCCA 1 cut(s) 798
BarI GAAGNNNNNNTAC 2 cut(s) 682, 714
BbvI GCAGC 2 cut(s) 839, 887
BceAI ACGGC 1 cut(s) 207
BciT130I CCWGG 2 cut(s) 1327, 1382
BclI TGATCA 3 cut(s) 109, 1204, 1366
BcnI CCSGG 2 cut(s) 177, 1359
BcoDI GTCTC 3 cut(s) 785, 1075, 1110
BfaI CTAG 3 cut(s) 519, 1002, 1200
BfoI RGCGCY 1 cut(s) 1242
BglII AGATCT 1 cut(s) 601
BisI GCNGC 2 cut(s) 853, 876
BlsI GCNGC 2 cut(s) 854, 877
Bme1390I CCNGG 4 cut(s) 177, 1327, 1359, 1382
Bme18I GGWCC 3 cut(s) 305, 319, 1271
BmgT120I GGNCC 4 cut(s) 220, 305, 319, 1271
BmrFI CCNGG 4 cut(s) 177, 1327, 1359, 1382
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 4 cut(s) 104, 256, 685, 1303
BmtI GCTAGC 1 cut(s) 522
BmuI ACTGGG 1 cut(s) 1045
BpmI CTGGAG 1 cut(s) 1143
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 481, 1227
BpuMI CCSGG 2 cut(s) 177, 1359
BsaI GGTCTC 1 cut(s) 1110
BsaJI CCNNGG 5 cut(s) 308, 1089, 1326, 1380, 1391
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 4 cut(s) 302, 835, 978, 1332
Bse118I RCCGGY 2 cut(s) 193, 285
Bse1I ACTGG 7 cut(s) 39, 322, 799, 1040, 1177, 1198, 1240
BseBI CCWGG 2 cut(s) 1327, 1382
BseDI CCNNGG 5 cut(s) 308, 1089, 1326, 1380, 1391
BseGI GGATG 5 cut(s) 431, 1056, 1084, 1294, 1336
BseLI CCNNNNNNNGG 4 cut(s) 302, 835, 978, 1332
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 7 cut(s) 39, 322, 799, 1040, 1177, 1198, 1240
BseRI GAGGAG 1 cut(s) 1420
BseXI GCAGC 2 cut(s) 839, 887
BseYI CCCAGC 2 cut(s) 878, 1060
BshFI GGCC 2 cut(s) 222, 798
BshTI ACCGGT 1 cut(s) 193
BsiSI CCGG 5 cut(s) 177, 194, 286, 813, 1358
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 4 cut(s) 302, 835, 978, 1332
BsmAI GTCTC 3 cut(s) 785, 1075, 1110
BsmFI GGGAC 1 cut(s) 906
BsnI GGCC 2 cut(s) 222, 798
Bso31I GGTCTC 1 cut(s) 1110
Bsp119I TTCGAA 1 cut(s) 356
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 1 cut(s) 1402
BspANI GGCC 2 cut(s) 222, 798
BspCNI CTCAG 1 cut(s) 361
BspHI TCATGA 2 cut(s) 112, 1264
BspOI GCTAGC 1 cut(s) 522
BspPI GGATC 4 cut(s) 562, 969, 1101, 1204
BspT104I TTCGAA 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 1110
BsrFI RCCGGY 2 cut(s) 193, 285
BsrI ACTGG 7 cut(s) 39, 322, 799, 1040, 1177, 1198, 1240
BssAI RCCGGY 2 cut(s) 193, 285
BssECI CCNNGG 5 cut(s) 308, 1089, 1326, 1380, 1391
BssT1I CCWWGG 3 cut(s) 308, 1089, 1391
Bst2UI CCWGG 2 cut(s) 1327, 1382
Bst4CI ACNGT 3 cut(s) 433, 504, 775
Bst6I CTCTTC 1 cut(s) 1262
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 520, 873
BstDEI CTNAG 2 cut(s) 137, 348
BstDSI CCRYGG 1 cut(s) 308
BstEII GGTNACC 1 cut(s) 189
BstENI CCTNNNNNAGG 1 cut(s) 833
BstF5I GGATG 5 cut(s) 431, 1056, 1084, 1294, 1336
BstH2I RGCGCY 1 cut(s) 1242
BstHHI GCGC 3 cut(s) 754, 1134, 1241
BstMAI GTCTC 3 cut(s) 785, 1075, 1110
BstMWI GCNNNNNNNGC 2 cut(s) 92, 1247
BstNI CCWGG 2 cut(s) 1327, 1382
BstNSI RCATGY 2 cut(s) 526, 710
BstPI GGTNACC 1 cut(s) 189
BstSCI CCNGG 4 cut(s) 175, 1325, 1357, 1380
BstV1I GCAGC 2 cut(s) 839, 887
BstX2I RGATCY 3 cut(s) 554, 601, 1196
BstXI CCANNNNNNTGG 1 cut(s) 1268
BstYI RGATCY 3 cut(s) 554, 601, 1196
BsuRI GGCC 2 cut(s) 222, 798
BtgI CCRYGG 1 cut(s) 308
BtsCI GGATG 5 cut(s) 431, 1056, 1084, 1294, 1336
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 5 cut(s) 46, 203, 823, 1170, 1191
Cac8I GCNNGC 2 cut(s) 520, 873
CciI TCATGA 2 cut(s) 112, 1264
CfoI GCGC 3 cut(s) 754, 1134, 1241
Cfr10I RCCGGY 2 cut(s) 193, 285
Cfr13I GGNCC 4 cut(s) 220, 305, 319, 1271
CseI GACGC 1 cut(s) 858
Csp6I GTAC 2 cut(s) 1057, 1183
CspAI ACCGGT 1 cut(s) 193
CviAII CATG 9 cut(s) 113, 275, 309, 523, 596, 707, 1067, 1265, 1309
CviQI GTAC 2 cut(s) 1057, 1183
DdeI CTNAG 2 cut(s) 137, 348
DrdI GACNNNNNNGTC 1 cut(s) 1124
DseDI GACNNNNNNGTC 1 cut(s) 1124
EaeI YGGCCR 1 cut(s) 796
Eam1104I CTCTTC 1 cut(s) 1262
EarI CTCTTC 1 cut(s) 1262
EciI GGCGGA 1 cut(s) 1417
Eco130I CCWWGG 3 cut(s) 308, 1089, 1391
Eco31I GGTCTC 1 cut(s) 1110
Eco47I GGWCC 3 cut(s) 305, 319, 1271
Eco91I GGTNACC 1 cut(s) 189
EcoNI CCTNNNNNAGG 1 cut(s) 833
EcoO65I GGTNACC 1 cut(s) 189
EcoRII CCWGG 2 cut(s) 1325, 1380
EcoT14I CCWWGG 3 cut(s) 308, 1089, 1391
ErhI CCWWGG 3 cut(s) 308, 1089, 1391
FaeI CATG 9 cut(s) 116, 278, 312, 526, 599, 710, 1070, 1268, 1312
FaqI GGGAC 1 cut(s) 906
FatI CATG 9 cut(s) 112, 274, 308, 522, 595, 706, 1066, 1264, 1308
FauNDI CATATG 2 cut(s) 340, 514
FbaI TGATCA 3 cut(s) 109, 1204, 1366
Fnu4HI GCNGC 2 cut(s) 853, 876
FokI GGATG 5 cut(s) 438, 1063, 1071, 1281, 1343
Fsp4HI GCNGC 2 cut(s) 853, 876
FspBI CTAG 3 cut(s) 519, 1002, 1200
FspI TGCGCA 1 cut(s) 1133
GlaI GCGC 3 cut(s) 753, 1133, 1240
GluI GCNGC 2 cut(s) 853, 876
GsaI CCCAGC 2 cut(s) 882, 1064
GsuI CTGGAG 1 cut(s) 1143
HaeII RGCGCY 1 cut(s) 1242
HaeIII GGCC 2 cut(s) 222, 798
HapII CCGG 5 cut(s) 177, 194, 286, 813, 1358
HgaI GACGC 1 cut(s) 858
HhaI GCGC 3 cut(s) 754, 1134, 1241
Hin1II CATG 9 cut(s) 116, 278, 312, 526, 599, 710, 1070, 1268, 1312
Hin6I GCGC 3 cut(s) 752, 1132, 1239
HinP1I GCGC 3 cut(s) 752, 1132, 1239
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HindIII AAGCTT 2 cut(s) 134, 457
HinfI GANTC 2 cut(s) 103, 569
HpaII CCGG 5 cut(s) 177, 194, 286, 813, 1358
HphI GGTGA 5 cut(s) 116, 121, 483, 519, 1243
Hpy166II GTNNAC 3 cut(s) 201, 319, 769
Hpy188I TCNGA 4 cut(s) 438, 763, 888, 966
Hpy188III TCNNGA 9 cut(s) 107, 113, 144, 605, 862, 899, 1002, 1265, 1342
Hpy8I GTNNAC 3 cut(s) 201, 319, 769
HpyAV CCTTC 6 cut(s) 6, 56, 433, 896, 952, 1011
HpyCH4III ACNGT 3 cut(s) 433, 504, 775
HpyCH4IV ACGT 3 cut(s) 758, 911, 937
HpyCH4V TGCA 5 cut(s) 338, 512, 578, 785, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 1247
HpyF3I CTNAG 2 cut(s) 137, 348
HpySE526I ACGT 3 cut(s) 758, 911, 937
Hsp92II CATG 9 cut(s) 116, 278, 312, 526, 599, 710, 1070, 1268, 1312
HspAI GCGC 3 cut(s) 752, 1132, 1239
Ksp22I TGATCA 3 cut(s) 109, 1204, 1366
Lsp1109I GCAGC 2 cut(s) 839, 887
LweI GCATC 4 cut(s) 104, 256, 685, 1303
MaeI CTAG 3 cut(s) 519, 1002, 1200
MaeII ACGT 3 cut(s) 758, 911, 937
MaeIII GTNAC 7 cut(s) 5, 189, 525, 570, 938, 1148, 1231
MboII GAAGA 7 cut(s) 92, 549, 611, 641, 1003, 1249, 1314
MflI RGATCY 3 cut(s) 554, 601, 1196
MlsI TGGCCA 1 cut(s) 798
MluCI AATT 5 cut(s) 232, 471, 632, 653, 1140
MluNI TGGCCA 1 cut(s) 798
MlyI GAGTC 2 cut(s) 112, 578
Mox20I TGGCCA 1 cut(s) 798
MroXI GAANNNNTTC 1 cut(s) 1256
MscI TGGCCA 1 cut(s) 798
MseI TTAA 5 cut(s) 281, 300, 651, 944, 1139
MslI CAYNNNNRTG 2 cut(s) 711, 1266
Msp20I TGGCCA 1 cut(s) 798
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 5 cut(s) 177, 194, 286, 813, 1358
MspR9I CCNGG 4 cut(s) 177, 1327, 1359, 1382
MvaI CCWGG 2 cut(s) 1327, 1382
MwoI GCNNNNNNNGC 2 cut(s) 92, 1247
NciI CCSGG 2 cut(s) 177, 1359
NcoI CCATGG 1 cut(s) 308
NdeI CATATG 2 cut(s) 340, 514
NheI GCTAGC 1 cut(s) 518
NlaIII CATG 9 cut(s) 116, 278, 312, 526, 599, 710, 1070, 1268, 1312
NmuCI GTSAC 3 cut(s) 525, 570, 1231
NsbI TGCGCA 1 cut(s) 1133
NspI RCATGY 2 cut(s) 526, 710
NspV TTCGAA 1 cut(s) 356
PagI TCATGA 2 cut(s) 112, 1264
PciI ACATGT 1 cut(s) 706
PdmI GAANNNNTTC 1 cut(s) 1256
PflFI GACNNNGTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 978
PfoI TCCNGGA 1 cut(s) 1357
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 2 cut(s) 854, 877
PleI GAGTC 2 cut(s) 111, 577
PpsI GAGTC 2 cut(s) 111, 577
PscI ACATGT 1 cut(s) 706
PshBI ATTAAT 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 911
Psp6I CCWGG 2 cut(s) 1325, 1380
PspEI GGTNACC 1 cut(s) 189
PspFI CCCAGC 2 cut(s) 878, 1060
PspGI CCWGG 2 cut(s) 1325, 1380
PspPI GGNCC 4 cut(s) 220, 305, 319, 1271
PsuI RGATCY 3 cut(s) 554, 601, 1196
PsyI GACNNNGTC 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 2 cut(s) 1058, 1184
RsaNI GTAC 2 cut(s) 1057, 1183
RseI CAYNNNNRTG 2 cut(s) 711, 1266
SaqAI TTAA 5 cut(s) 281, 300, 651, 944, 1139
SatI GCNGC 2 cut(s) 853, 876
Sau96I GGNCC 4 cut(s) 220, 305, 319, 1271
SchI GAGTC 2 cut(s) 112, 578
ScrFI CCNGG 4 cut(s) 177, 1327, 1359, 1382
SfaNI GCATC 4 cut(s) 104, 256, 685, 1303
SfuI TTCGAA 1 cut(s) 356
SinI GGWCC 3 cut(s) 305, 319, 1271
SmiMI CAYNNNNRTG 2 cut(s) 711, 1266
SmlI CTYRAG 2 cut(s) 460, 1242
SmoI CTYRAG 2 cut(s) 460, 1242
Sse9I AATT 5 cut(s) 232, 471, 632, 653, 1140
SsiI CCGC 1 cut(s) 1402
SspI AATATT 1 cut(s) 642
SspMI CTAG 3 cut(s) 519, 1002, 1200
StyD4I CCNGG 4 cut(s) 175, 1325, 1357, 1380
StyI CCWWGG 3 cut(s) 308, 1089, 1391
TaaI ACNGT 3 cut(s) 433, 504, 775
TaiI ACGT 3 cut(s) 761, 914, 940
TaqI TCGA 2 cut(s) 356, 1015
TaqII GACCGA 1 cut(s) 1201
TasI AATT 5 cut(s) 232, 471, 632, 653, 1140
TatI WGTACW 1 cut(s) 1182
Tru1I TTAA 5 cut(s) 281, 300, 651, 944, 1139
Tru9I TTAA 5 cut(s) 281, 300, 651, 944, 1139
TscAI CASTG 5 cut(s) 46, 210, 823, 1177, 1198
TseFI GTSAC 3 cut(s) 525, 570, 1231
TseI GCWGC 2 cut(s) 852, 875
Tsp45I GTSAC 3 cut(s) 525, 570, 1231
TspDTI ATGAA 4 cut(s) 105, 129, 612, 642
TspGWI ACGGA 2 cut(s) 602, 1003
TspRI CASTG 5 cut(s) 46, 210, 823, 1177, 1198
Tth111I GACNNNGTC 1 cut(s) 568
Van91I CCANNNNNTGG 1 cut(s) 978
VpaK11BI GGWCC 3 cut(s) 305, 319, 1271
VspI ATTAAT 1 cut(s) 300
XagI CCTNNNNNAGG 1 cut(s) 833
XapI RAATTY 2 cut(s) 632, 653
XbaI TCTAGA 1 cut(s) 1001
XceI RCATGY 2 cut(s) 526, 710
XmnI GAANNNNTTC 1 cut(s) 1256
XspI CTAG 3 cut(s) 519, 1002, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.