MD03G1273100.v1.1

LEC14B protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001751
Physical Location & Seq
Reverse (-)
35634859 .. 35640172
5314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1273100.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGGGTATTCACTCAGTAGATTGGCACTAGAATCTGATCATTTTGATCCTGGAAATGCTGCCGTCCATGGAGTCGGTAACGGTCAAACACCCAACAAACCATTGCATAATCTAGAGCATGAAGTAGCCCAGCTCACAAAGTTGAGGTCAGCTCCCAATGAGCGGCTTACCCAAATTGGACCTAGAAGGCGGGACTTGCCCGTTTCCACGGTCAGGATGCTGGCTGGTCGAGAGGGCAATTATTCGGGAAGAGGAGGCAGGTTCTCTTCTGCAGATCGTTGCCATGTTCTAAGTAGATATTTGCCAGTCAATGGTCCTTGGCCTGTCGGCCAAATGTCTAGCCGAGCTTATGTCTCTCAGTTTTCGGCAGATGGATCCCTTTTCATTGCTGGGTTTCAGGATAGCCATATTAAAATATACAATGTGGATAGAGAGTGGAAAGTCCAGAAGAACATTCATGCCAAAAGTATGCGATGGACTGTCACTGATACGTCTCTTTCCCCGGATCAACGCCATCTTGTTTATGCGAGTATGTCCCCTATTGTTCATATGGTTGATGTGGGATCTTCTGAAGTGGAGTCTCATGCAAATATAACGGAGATCCACGAGGGTTTGGATTTTTCCTCTGGTGATGACGGAGGGTACTCATTTGGTATTTTCTCCATAAAATTCTCAACAGATGGACATGAACTTGTTGCTGGAAGTAGCGATGATTCCATATATGTTTATGATCTTGAAGCCAATATGCTATCCCTTCGGATTCCAGCGCACGAGTCTGATGTGAACACCGTATGCTTTGCCGATGAAGGTGGCCATCTTATTTATTCTGGGAGTGATGATACTCTCTGTAAGGTATGGGATAGACGTTGCTTTAACGCCACAAGGAAGCCAGCAGGAGTCTTGACTGGACACCTAGAAGGTATTGCATTCATTGACAGCAAAGGAGATGGTCGTTATTTCATTTCGAATGGTAAAGATCAGACCATTAAACTTTGGGATATCAGGAAAATGTCTTCTAGTCCCGCTTGCACTCCAGGGGTCAGGAGTTCTGAGTGGGATTACAGATGGATGGACTACCCACCCCAGGCAAAAGATAAGAAACATCCGTCTGATCAATCAGTAGCTACGTATAGAGGACATTCAGTCTTGCGTACGCTTATTCGCTGCTACTTTTCTCCGGCATATAGCACCGGTCAGAAGTACATATACACGGGATCGCATAACTGTTCTGTGTATATATATGATTTGGTTACCGGAGCCCAAGTTGCGACGCTGAAGTATCATAAAGCACCGGTGAGGGACTGTAGTTGGCACCCCTATTACCCGATGCTCGTAAGCTCTTCTTGGGACGGGAAGGTTGCTAGATGGGAGATCCCCGGCAGTGGAGGAGCGCCGGCCTCATTGTATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

471

Amino Acids

52.25

Weight (kDa)

7.04

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 120 - 291 1.6e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 121 - 293 3.4e-14 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 128 - 287 3.4e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 131 - 283 2.7e-07 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 213 - 290 1.5e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 214 - 333 4.8e-13 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 219 - 287 5.6e-06 WDHD1 first WD40 domain
WDR55 PF24796 219 - 336 5.3e-14 WDR55
Beta-prop_EML_2 PF23414 219 - 353 1.8e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 219 - 339 1.3e-13 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 219 - 338 6.8e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 219 - 334 2.6e-17 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 230 - 342 6e-08 TEP-1 second beta-propeller
EIF3I PF24805 252 - 451 2.4e-10 EIF3I
WD40 PF00400 254 - 287 5.5e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 371 - 456 1.5e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 394 - 458 5.3e-09 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 395 - 458 6.4e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 398 - 460 1.4e-07 Prp19 WD40 domain
WD40 PF00400 420 - 456 3.4e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 1311
AccB7I CCANNNNNTGG 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 163
AciI CCGC 3 cut(s) 163, 190, 1023
AclWI GGATC 8 cut(s) 41, 369, 382, 513, 571, 595, 1222, 1366
AcoI YGGCCR 2 cut(s) 328, 811
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 2 cut(s) 591, 1295
AfaI GTAC 3 cut(s) 644, 1153, 1202
AfiI CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
AgeI ACCGGT 2 cut(s) 1190, 1291
AgsI TTSAA 1 cut(s) 737
AhdI GACNNNNNGTC 1 cut(s) 1142
AjnI CCWGG 3 cut(s) 49, 1033, 1083
AluBI AGCT 5 cut(s) 133, 152, 347, 1124, 1338
AluI AGCT 5 cut(s) 133, 152, 347, 1124, 1338
Alw26I GTCTC 3 cut(s) 358, 498, 585
AlwI GGATC 8 cut(s) 41, 369, 382, 513, 571, 595, 1222, 1366
AoxI GGCC 4 cut(s) 320, 328, 811, 1395
ApeKI GCWGC 2 cut(s) 59, 1164
ApoI RAATTY 1 cut(s) 668
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AsiGI ACCGGT 2 cut(s) 1190, 1291
AspLEI GCGC 2 cut(s) 769, 1393
AspS9I GGNCC 2 cut(s) 179, 314
AsuC2I CCSGG 2 cut(s) 503, 1377
AsuHPI GGTGA 2 cut(s) 641, 1306
AsuII TTCGAA 1 cut(s) 965
AvaII GGWCC 2 cut(s) 179, 314
BalI TGGCCA 1 cut(s) 813
BamHI GGATCC 1 cut(s) 374
BanI GGYRCC 1 cut(s) 1311
BanII GRGCYC 1 cut(s) 1261
BarI GAAGNNNNNNTAC 2 cut(s) 1190, 1222
BauI CACGAG 2 cut(s) 605, 770
BbsI GAAGAC 1 cut(s) 1005
BbvI GCAGC 2 cut(s) 46, 1151
BccI CCATC 9 cut(s) 365, 468, 522, 674, 822, 941, 1059, 1063, 1359
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 3 cut(s) 51, 1035, 1085
BclI TGATCA 2 cut(s) 37, 1111
BcnI CCSGG 2 cut(s) 503, 1377
BcoDI GTCTC 3 cut(s) 358, 498, 585
BfaI CTAG 7 cut(s) 29, 113, 183, 339, 914, 1017, 1362
BfmI CTRYAG 2 cut(s) 270, 1303
BfoI RGCGCY 1 cut(s) 1394
BfuAI ACCTGC 1 cut(s) 249
BisI GCNGC 3 cut(s) 60, 164, 1165
BlsI GCNGC 3 cut(s) 61, 165, 1166
Bme1390I CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
Bme18I GGWCC 2 cut(s) 179, 314
BmeRI GACNNNNNGTC 1 cut(s) 1142
BmgT120I GGNCC 2 cut(s) 179, 314
BmiI GGNNCC 3 cut(s) 376, 1258, 1313
BmrFI CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
BmsI GCATC 2 cut(s) 207, 1317
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 1017
Bpu14I TTCGAA 1 cut(s) 965
BpuMI CCSGG 2 cut(s) 503, 1377
BsaAI YACGTR 1 cut(s) 1128
BsaJI CCNNGG 7 cut(s) 67, 207, 317, 501, 1034, 1083, 1375
BsaWI WCCGGW 3 cut(s) 1190, 1253, 1291
BsaXI ACNNNNNCTCC 2 cut(s) 1248, 1278
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
Bse118I RCCGGY 3 cut(s) 1190, 1291, 1393
Bse1I ACTGG 2 cut(s) 305, 910
Bse3DI GCAATG 2 cut(s) 101, 384
BseBI CCWGG 3 cut(s) 51, 1035, 1085
BseDI CCNNGG 7 cut(s) 67, 207, 317, 501, 1034, 1083, 1375
BseGI GGATG 3 cut(s) 222, 1074, 1102
BseLI CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
BseMI GCAATG 2 cut(s) 101, 384
BseMII CTCAG 3 cut(s) 28, 371, 1041
BseNI ACTGG 2 cut(s) 305, 910
BseRI GAGGAG 2 cut(s) 267, 1401
BseXI GCAGC 2 cut(s) 46, 1151
BseYI CCCAGC 2 cut(s) 129, 389
BshFI GGCC 4 cut(s) 322, 330, 813, 1397
BshNI GGYRCC 1 cut(s) 1311
BshTI ACCGGT 2 cut(s) 1190, 1291
BsiSI CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
BsiWI CGTACG 1 cut(s) 1151
BslFI GGGAC 5 cut(s) 206, 520, 1005, 1313, 1361
BslI CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
BsmAI GTCTC 3 cut(s) 358, 498, 585
BsmBI CGTCTC 1 cut(s) 498
BsmFI GGGAC 5 cut(s) 206, 520, 1005, 1313, 1361
BsmI GAATGC 1 cut(s) 926
BsnI GGCC 4 cut(s) 322, 330, 813, 1397
Bsp119I TTCGAA 1 cut(s) 965
Bsp1286I GDGCHC 1 cut(s) 1261
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 3 cut(s) 163, 190, 1023
BspANI GGCC 4 cut(s) 322, 330, 813, 1397
BspCNI CTCAG 3 cut(s) 27, 370, 1042
BspLI GGNNCC 3 cut(s) 376, 1258, 1313
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 249
BspPI GGATC 8 cut(s) 41, 369, 382, 513, 571, 595, 1222, 1366
BspQI GCTCTTC 1 cut(s) 1345
BspT104I TTCGAA 1 cut(s) 965
BspT107I GGYRCC 1 cut(s) 1311
BsrBI CCGCTC 1 cut(s) 163
BsrDI GCAATG 2 cut(s) 101, 384
BsrFI RCCGGY 3 cut(s) 1190, 1291, 1393
BsrI ACTGG 2 cut(s) 305, 910
BssAI RCCGGY 3 cut(s) 1190, 1291, 1393
BssECI CCNNGG 7 cut(s) 67, 207, 317, 501, 1034, 1083, 1375
BssSI CACGAG 2 cut(s) 605, 770
BssT1I CCWWGG 2 cut(s) 67, 317
Bst2BI CACGAG 2 cut(s) 605, 770
Bst2UI CCWGG 3 cut(s) 51, 1035, 1085
Bst4CI ACNGT 6 cut(s) 83, 211, 481, 790, 1226, 1304
Bst6I CTCTTC 3 cut(s) 244, 271, 1345
BstBAI YACGTR 1 cut(s) 1128
BstBI TTCGAA 1 cut(s) 965
BstC8I GCNNGC 4 cut(s) 222, 891, 1027, 1395
BstDEI CTNAG 4 cut(s) 14, 290, 357, 1050
BstDSI CCRYGG 2 cut(s) 67, 207
BstEII GGTNACC 1 cut(s) 1249
BstF5I GGATG 3 cut(s) 222, 1074, 1102
BstH2I RGCGCY 1 cut(s) 1394
BstHHI GCGC 2 cut(s) 769, 1393
BstMAI GTCTC 3 cut(s) 358, 498, 585
BstMWI GCNNNNNNNGC 2 cut(s) 196, 1265
BstNI CCWGG 3 cut(s) 51, 1035, 1085
BstPI GGTNACC 1 cut(s) 1249
BstSCI CCNGG 5 cut(s) 49, 501, 1033, 1083, 1375
BstSFI CTRYAG 2 cut(s) 270, 1303
BstSNI TACGTA 1 cut(s) 1128
BstV1I GCAGC 2 cut(s) 46, 1151
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 4 cut(s) 374, 563, 600, 1371
BstYI RGATCY 4 cut(s) 374, 563, 600, 1371
BsuRI GGCC 4 cut(s) 322, 330, 813, 1397
BtgI CCRYGG 2 cut(s) 67, 207
BtgZI GCGATG 2 cut(s) 487, 723
BtsCI GGATG 3 cut(s) 222, 1074, 1102
BtsI GCAGTG 1 cut(s) 1387
BtsIMutI CAGTG 2 cut(s) 483, 1387
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 4 cut(s) 222, 891, 1027, 1395
CfoI GCGC 2 cut(s) 769, 1393
Cfr10I RCCGGY 3 cut(s) 1190, 1291, 1393
Cfr13I GGNCC 2 cut(s) 179, 314
CseI GACGC 1 cut(s) 1279
Csp6I GTAC 3 cut(s) 643, 1152, 1201
CspAI ACCGGT 2 cut(s) 1190, 1291
CviAII CATG 6 cut(s) 68, 119, 284, 458, 584, 686
CviQI GTAC 3 cut(s) 643, 1152, 1201
DdeI CTNAG 4 cut(s) 14, 290, 357, 1050
DriI GACNNNNNGTC 1 cut(s) 1142
EaeI YGGCCR 2 cut(s) 328, 811
Eam1104I CTCTTC 3 cut(s) 244, 271, 1345
Eam1105I GACNNNNNGTC 1 cut(s) 1142
EarI CTCTTC 3 cut(s) 244, 271, 1345
Eco105I TACGTA 1 cut(s) 1128
Eco130I CCWWGG 2 cut(s) 67, 317
Eco24I GRGCYC 1 cut(s) 1261
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 2 cut(s) 179, 314
Eco57I CTGAAG 2 cut(s) 591, 1295
Eco91I GGTNACC 1 cut(s) 1249
EcoO65I GGTNACC 1 cut(s) 1249
EcoRII CCWGG 3 cut(s) 49, 1033, 1083
EcoRV GATATC 1 cut(s) 1000
EcoT14I CCWWGG 2 cut(s) 67, 317
EcoT38I GRGCYC 1 cut(s) 1261
ErhI CCWWGG 2 cut(s) 67, 317
Esp3I CGTCTC 1 cut(s) 498
FaeI CATG 6 cut(s) 71, 122, 287, 461, 587, 689
FalI AAGNNNNNCTT 2 cut(s) 1327, 1359
FaqI GGGAC 5 cut(s) 206, 520, 1005, 1313, 1361
FatI CATG 6 cut(s) 67, 118, 283, 457, 583, 685
FauI CCCGC 2 cut(s) 183, 1030
FauNDI CATATG 1 cut(s) 549
FbaI TGATCA 2 cut(s) 37, 1111
Fnu4HI GCNGC 3 cut(s) 60, 164, 1165
FokI GGATG 3 cut(s) 229, 1081, 1089
FriOI GRGCYC 1 cut(s) 1261
Fsp4HI GCNGC 3 cut(s) 60, 164, 1165
FspBI CTAG 7 cut(s) 29, 113, 183, 339, 914, 1017, 1362
GlaI GCGC 2 cut(s) 768, 1392
GluI GCNGC 3 cut(s) 60, 164, 1165
GsaI CCCAGC 2 cut(s) 133, 393
GsuI CTGGAG 1 cut(s) 1017
HaeII RGCGCY 1 cut(s) 1394
HaeIII GGCC 4 cut(s) 322, 330, 813, 1397
HapII CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
HgaI GACGC 1 cut(s) 1279
HhaI GCGC 2 cut(s) 769, 1393
Hin1II CATG 6 cut(s) 71, 122, 287, 461, 587, 689
Hin6I GCGC 2 cut(s) 767, 1391
HinP1I GCGC 2 cut(s) 767, 1391
HinfI GANTC 7 cut(s) 32, 72, 578, 713, 760, 773, 897
HpaII CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
HphI GGTGA 2 cut(s) 641, 1306
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 8 cut(s) 37, 571, 759, 778, 981, 1051, 1111, 1197
Hpy8I GTNNAC 1 cut(s) 784
Hpy99I CGWCG 1 cut(s) 1273
HpyAV CCTTC 5 cut(s) 180, 764, 800, 911, 1348
HpyCH4III ACNGT 6 cut(s) 83, 211, 481, 790, 1226, 1304
HpyCH4IV ACGT 3 cut(s) 491, 865, 1127
HpyCH4V TGCA 5 cut(s) 106, 272, 587, 926, 1029
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 1265
HpyF3I CTNAG 4 cut(s) 14, 290, 357, 1050
HpySE526I ACGT 3 cut(s) 491, 865, 1127
Hsp92II CATG 6 cut(s) 71, 122, 287, 461, 587, 689
HspAI GCGC 2 cut(s) 767, 1391
KroI GCCGGC 1 cut(s) 1393
KroNI GCCGGC 1 cut(s) 1395
Ksp22I TGATCA 2 cut(s) 37, 1111
LguI GCTCTTC 1 cut(s) 1345
LmnI GCTCC 3 cut(s) 157, 1256, 1388
Lsp1109I GCAGC 2 cut(s) 46, 1151
LweI GCATC 2 cut(s) 207, 1317
MaeI CTAG 7 cut(s) 29, 113, 183, 339, 914, 1017, 1362
MaeII ACGT 3 cut(s) 491, 865, 1127
MaeIII GTNAC 3 cut(s) 77, 481, 1249
MbiI CCGCTC 1 cut(s) 163
MboII GAAGA 6 cut(s) 258, 261, 460, 558, 1005, 1332
MflI RGATCY 4 cut(s) 374, 563, 600, 1371
MhlI GDGCHC 1 cut(s) 1261
MlsI TGGCCA 1 cut(s) 813
MluCI AATT 3 cut(s) 174, 238, 668
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 4 cut(s) 81, 587, 782, 906
Mox20I TGGCCA 1 cut(s) 813
MroNI GCCGGC 1 cut(s) 1393
MscI TGGCCA 1 cut(s) 813
MseI TTAA 4 cut(s) 411, 873, 987, 1411
Msp20I TGGCCA 1 cut(s) 813
MspI CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
MspR9I CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
Mva1269I GAATGC 1 cut(s) 926
MvaI CCWGG 3 cut(s) 51, 1035, 1085
MwoI GCNNNNNNNGC 2 cut(s) 196, 1265
NaeI GCCGGC 1 cut(s) 1395
NciI CCSGG 2 cut(s) 503, 1377
NcoI CCATGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 549
NgoMIV GCCGGC 1 cut(s) 1393
NlaIII CATG 6 cut(s) 71, 122, 287, 461, 587, 689
NlaIV GGNNCC 3 cut(s) 376, 1258, 1313
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 481
NspV TTCGAA 1 cut(s) 965
PciSI GCTCTTC 1 cut(s) 1345
PctI GAATGC 1 cut(s) 926
PdiI GCCGGC 1 cut(s) 1395
PfeI GAWTC 3 cut(s) 32, 713, 760
Pfl23II CGTACG 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 49
PinAI ACCGGT 2 cut(s) 1190, 1291
PkrI GCNGC 3 cut(s) 61, 165, 1166
PleI GAGTC 4 cut(s) 80, 586, 781, 905
PpsI GAGTC 4 cut(s) 80, 586, 781, 905
Ppu21I YACGTR 1 cut(s) 1128
Psp6I CCWGG 3 cut(s) 49, 1033, 1083
PspEI GGTNACC 1 cut(s) 1249
PspFI CCCAGC 2 cut(s) 129, 389
PspGI CCWGG 3 cut(s) 49, 1033, 1083
PspLI CGTACG 1 cut(s) 1151
PspN4I GGNNCC 3 cut(s) 376, 1258, 1313
PspPI GGNCC 2 cut(s) 179, 314
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 4 cut(s) 374, 563, 600, 1371
RsaI GTAC 3 cut(s) 644, 1153, 1202
RsaNI GTAC 3 cut(s) 643, 1152, 1201
SapI GCTCTTC 1 cut(s) 1345
SaqAI TTAA 4 cut(s) 411, 873, 987, 1411
SatI GCNGC 3 cut(s) 60, 164, 1165
Sau96I GGNCC 2 cut(s) 179, 314
SchI GAGTC 4 cut(s) 81, 587, 782, 906
ScrFI CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
SduI GDGCHC 1 cut(s) 1261
SfaNI GCATC 2 cut(s) 207, 1317
SfcI CTRYAG 2 cut(s) 270, 1303
SfuI TTCGAA 1 cut(s) 965
SgrAI CRCCGGYG 1 cut(s) 1291
SinI GGWCC 2 cut(s) 179, 314
SnaBI TACGTA 1 cut(s) 1128
Sse9I AATT 3 cut(s) 174, 238, 668
SsiI CCGC 3 cut(s) 163, 190, 1023
SspMI CTAG 7 cut(s) 29, 113, 183, 339, 914, 1017, 1362
StyD4I CCNGG 5 cut(s) 49, 501, 1033, 1083, 1375
StyI CCWWGG 2 cut(s) 67, 317
TaaI ACNGT 6 cut(s) 83, 211, 481, 790, 1226, 1304
TaiI ACGT 3 cut(s) 494, 868, 1130
TaqI TCGA 2 cut(s) 229, 965
TasI AATT 3 cut(s) 174, 238, 668
TatI WGTACW 1 cut(s) 1200
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 3 cut(s) 32, 713, 760
Tru1I TTAA 4 cut(s) 411, 873, 987, 1411
Tru9I TTAA 4 cut(s) 411, 873, 987, 1411
TscAI CASTG 2 cut(s) 490, 1387
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 2 cut(s) 59, 1164
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 8 cut(s) 135, 373, 446, 536, 702, 819, 919, 949
TspGWI ACGGA 3 cut(s) 611, 651, 1095
TspRI CASTG 2 cut(s) 490, 1387
Van91I CCANNNNNTGG 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 179, 314
XapI RAATTY 1 cut(s) 668
XbaI TCTAGA 1 cut(s) 112
XspI CTAG 7 cut(s) 29, 113, 183, 339, 914, 1017, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.