Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001751

Physical Location & Seq

Genomic Coordinates Forward (+)

14552745 .. 14557783

Gene Structure 5039 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1176000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGAGTTACAGAGCGAGGTTTGGTAAAGATAATAGCGCCTGTGATAGCGGAAATTCTGCTGAAGGTTCCGGTTCAAGTAAAGGACCTGATGAAGTATCCAATGATTTTGATCATGAAATTGCTCAACTCACTAAGCATAGATCAAGACCCCACCGACTTTTGAGCGGGGATATGGCTGGGAAGTCGAGGTTACCGGTTTCAACGATGAAAATGCTGGCCGGCCGTGAAAGTAATTATTCAGGGCGGGGAAGATTCTCGTTGGCAGATTGTTGCCATGTTTTGAGCCGGTATCTGCCCATAAATGGTCCTTGGGGGGTGGACCAGTCCAGAAGTCCTGCTTATGTTTCTCAATTTTCGAATGATGGTTTGGCTTTTGTTGCTGGATTTCAGGGAGGCCATATTAGAATATATAATGTTGAAAAGGGATGGAAGGTTCAGAAGGATATCCTGACCAAAACCTTGAGATGGACTATTACCGATACATCTCTATCACCGGACCAACGTTATCTTGTTTATGCTAGCATGACACCGGTTGTCAATATTGTGAATGTGGGATCTTCGACGACGGTGTCAGTTGCAAATGTTATGGAAGTTCATGAAGGTCTGGATTTTTCTATTGAAGGCGATGAGGATGAATTTGGAATTTTCTCAGTTAAATTTTCAACTGATGGGCGAGAGCTTGTTGCTGCAAGTAGTGATGCTTCAATATATGTTTATGATCTCCAAGCAAATAAAGTTAGCCTCCGAATACCAGCTCACCAGTCTGATGTAAACACTGTGTGCTTTGCTGATGAGACCGGCCATCTCATATATTCTGGCAGTGACGACAACCTCTGTAAGGTGTGGGACAGACGCTGCTTAATGACGTACGGACAAGCATCTGGGGTCCTGATGGGACATCTTGAAGGTGTTACATTTATTGATAGCCGGGGAGATGGGCGTTACTTAATATCAAACGGGAAGGACCAGACAACCAAACTCTGGGATATAAGAAAGATGTCCTCTAGAGCCATGTACCCAAGGCTTACAGATCGTGACTGGGATTACAGATGGATGGAGTACCCAGCTCATGCAAGAACTCTGAAACATCCAAATGATCAGTCATTGGCTACATATAGAGGCCATGCAGTCCTGCGCACTTTAATTCGCTGTTACTTTTCTCCAGCATATAGTACTGGACAAAAGTACATCTACACTGGCTCAAGTGATCATTCCGTCTATATATATGATCTGGTAACCGGAGCTCAAGTTGCGAAACTAGATCATCATGAAGGACCTGTGAGAGACTGTAGTTGGCATCCTCTGTATCCCATTTTGGTCAGCTCGTCTTGGGACGGGACCGTCGCCCGATGGGAATTTCCTGGTGATGACCAAGTTCCCACCCTCGTGAGGCCGAAAATACGCCGGAGGAGAGGCTTCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

475

Amino Acids

53.18

Weight (kDa)

7.65

Isoelectric Point (pI)

44.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	121 - 332	1.9e-12	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	125 - 287	3.4e-13	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	126 - 282	6.8e-11	WDR36/Utp21 first beta-propeller
WDR55	PF24796	159 - 285	1.7e-12	WDR55
WD40_MABP1-WDR62_2nd	PF24782	160 - 284	4.5e-07	MABP1/WDR62 second WD40 domain
WD40_Gbeta	PF25391	179 - 286	1.2e-08	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	180 - 338	4e-15	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	211 - 333	1.2e-17	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	214 - 334	9.8e-20	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	215 - 348	6.7e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	215 - 285	3.6e-11	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	216 - 283	7.1e-06	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	227 - 340	1.8e-08	TEP-1 second beta-propeller
WD40	PF00400	249 - 283	5.2e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	298 - 461	5.5e-11	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	362 - 451	5.5e-11	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	389 - 454	8.5e-07	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	389 - 464	2e-10	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	393 - 457	5.2e-09	Prp19 WD40 domain
WD40	PF00400	416 - 451	2.1e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001751)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03020 AT4G03020 AT4G03020
fragaria_vesca	FvH4_2g24350 FvH4_2g24350 FvH4_2g24350 FvH4_4g16870 FvH4_4g16870
malus_domestica	MD03G1273100.v1.1 MD11G1294100.v1.1 MD13G1176000.v1.1 MD16G1177700.v1.1
prunus_persica	Prupe.1G145000_v2.0.a1 Prupe.1G145000_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1 Prupe.8G249600_v2.0.a1
pyrus_communis	pycom03g21760 pycom11g26020 pycom13g15170
rosa_chinensis	RchiOBHm_Chr4g0420461 RchiOBHm_Chr6g0292221
rosa_laevigata	RLG00000007761 RLG00000012052
rosa_multiflora	Rmu_co8469755.1_g000001 Rmu_sc0002248.1_g000028 Rmu_sc0002993.1_g000006 Rmu_sc0017106.1_g000005
rosa_roxburghii	Rroxscaffold_5G00363080 Rroxscaffold_7G00175340
rosa_rugosa	Rorug04G0163900 Rorug06G0224500 Rorug06G0224500
rosa_samantha	Rh4CG239900 Rh6AG337600 Rh6BG344900 Rh6CG352000
rosa_wichuraiana	Rw4G019470 Rw6G029400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	568, 1340
Acc16I	TGCGCA	1 cut(s)	1136
AccB7I	CCANNNNNTGG	1 cut(s)	981
AccBSI	CCGCTC	1 cut(s)	165
AciI	CCGC	3 cut(s)	48, 165, 244
AclI	AACGTT	1 cut(s)	502
AclWI	GGATC	1 cut(s)	562
AcoI	YGGCCR	3 cut(s)	216, 220, 799
AcsI	RAATTY	5 cut(s)	52, 635, 642, 656, 1355
AcuI	CTGAAG	1 cut(s)	81
AdeI	CACNNNGTG	1 cut(s)	780
AfaI	GTAC	5 cut(s)	869, 1016, 1061, 1174, 1187
AfiI	CCNNNNNNNGG	5 cut(s)	302, 465, 838, 981, 1389
AgeI	ACCGGT	2 cut(s)	193, 529
AgsI	TTSAA	7 cut(s)	75, 201, 419, 620, 663, 705, 905
AjnI	CCWGG	1 cut(s)	1360
AjuI	GAANNNNNNNTTGG	2 cut(s)	350, 382
AleI	CACNNNNGTG	1 cut(s)	1385
AloI	GAACNNNNNNTCC	2 cut(s)	417, 449
AluBI	AGCT	5 cut(s)	679, 755, 1067, 1244, 1323
AluI	AGCT	5 cut(s)	679, 755, 1067, 1244, 1323
Alw21I	GWGCWC	1 cut(s)	1246
Alw26I	GTCTC	2 cut(s)	788, 1278
AlwI	GGATC	1 cut(s)	562
AlwNI	CAGNNNCTG	1 cut(s)	855
AoxI	GGCC	6 cut(s)	216, 220, 394, 799, 1120, 1391
ApeKI	GCWGC	2 cut(s)	686, 855
ApoI	RAATTY	5 cut(s)	52, 635, 642, 656, 1355
ArsI	GACNNNNNNTTYG	2 cut(s)	1085, 1117
AsiGI	ACCGGT	2 cut(s)	193, 529
AspLEI	GCGC	2 cut(s)	38, 1137
AspS9I	GGNCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
AsuC2I	CCSGG	1 cut(s)	929
AsuHPI	GGTGA	3 cut(s)	483, 749, 1376
AsuII	TTCGAA	1 cut(s)	356
AsuNHI	GCTAGC	1 cut(s)	518
AvaII	GGWCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
BanII	GRGCYC	1 cut(s)	1246
BarI	GAAGNNNNNNTAC	2 cut(s)	685, 717
BauI	CACGAG	1 cut(s)	1385
Bbv12I	GWGCWC	1 cut(s)	1246
BbvI	GCAGC	2 cut(s)	673, 842
BceAI	ACGGC	1 cut(s)	207
BcgI	CGANNNNNNTGC	2 cut(s)	193, 227
BciT130I	CCWGG	1 cut(s)	1362
BciVI	GTATCC	2 cut(s)	106, 1317
BclI	TGATCA	3 cut(s)	109, 1096, 1207
BcnI	CCSGG	1 cut(s)	929
BcoDI	GTCTC	2 cut(s)	788, 1278
BfaI	CTAG	3 cut(s)	519, 1005, 1259
BfmI	CTRYAG	1 cut(s)	1288
BfoI	RGCGCY	1 cut(s)	39
BfuI	GTATCC	2 cut(s)	106, 1317
BisI	GCNGC	2 cut(s)	687, 856
BlsI	GCNGC	2 cut(s)	688, 857
BmcAI	AGTACT	1 cut(s)	1174
Bme1390I	CCNGG	2 cut(s)	929, 1362
Bme18I	GGWCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
BmgT120I	GGNCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
BmiI	GGNNCC	3 cut(s)	67, 887, 1339
BmrFI	CCNGG	2 cut(s)	929, 1362
BmrI	ACTGGG	1 cut(s)	1048
BmsI	GCATC	3 cut(s)	688, 887, 1306
BmtI	GCTAGC	1 cut(s)	522
BmuI	ACTGGG	1 cut(s)	1048
BpmI	CTGGAG	1 cut(s)	1146
Bpu14I	TTCGAA	1 cut(s)	356
BpuEI	CTTGAG	3 cut(s)	481, 1186, 1230
BpuMI	CCSGG	1 cut(s)	929
BsaBI	GATNNNNATC	1 cut(s)	108
BsaI	GGTCTC	1 cut(s)	788
BsaJI	CCNNGG	3 cut(s)	308, 928, 1019
BsaWI	WCCGGW	5 cut(s)	68, 193, 493, 529, 1238
BsaXI	ACNNNNNCTCC	2 cut(s)	1233, 1263
Bsc4I	CCNNNNNNNGG	5 cut(s)	302, 465, 838, 981, 1389
Bse118I	RCCGGY	5 cut(s)	193, 218, 285, 529, 797
Bse1I	ACTGG	5 cut(s)	322, 760, 1043, 1180, 1201
Bse8I	GATNNNNATC	1 cut(s)	108
BseBI	CCWGG	1 cut(s)	1362
BseDI	CCNNGG	3 cut(s)	308, 928, 1019
BseGI	GGATG	5 cut(s)	431, 637, 1059, 1087, 1297
BseJI	GATNNNNATC	1 cut(s)	108
BseLI	CCNNNNNNNGG	5 cut(s)	302, 465, 838, 981, 1389
BseMII	CTCAG	1 cut(s)	663
BseNI	ACTGG	5 cut(s)	322, 760, 1043, 1180, 1201
BseRI	GAGGAG	1 cut(s)	1423
BseX3I	CGGCCG	1 cut(s)	220
BseXI	GCAGC	2 cut(s)	673, 842
BseYI	CCCAGC	2 cut(s)	176, 1063
Bsh1285I	CGRYCG	1 cut(s)	223
BshFI	GGCC	6 cut(s)	218, 222, 396, 801, 1122, 1393
BshTI	ACCGGT	2 cut(s)	193, 529
BsiEI	CGRYCG	1 cut(s)	223
BsiHKAI	GWGCWC	1 cut(s)	1246
BsiWI	CGTACG	1 cut(s)	867
BslFI	GGGAC	4 cut(s)	860, 909, 1346, 1351
BslI	CCNNNNNNNGG	5 cut(s)	302, 465, 838, 981, 1389
BsmAI	GTCTC	2 cut(s)	788, 1278
BsmFI	GGGAC	4 cut(s)	860, 909, 1346, 1351
BsnI	GGCC	6 cut(s)	218, 222, 396, 801, 1122, 1393
Bso31I	GGTCTC	1 cut(s)	788
Bsp119I	TTCGAA	1 cut(s)	356
Bsp1286I	GDGCHC	1 cut(s)	1246
Bsp143I	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
BspACI	CCGC	3 cut(s)	48, 165, 244
BspANI	GGCC	6 cut(s)	218, 222, 396, 801, 1122, 1393
BspCNI	CTCAG	1 cut(s)	662
BspHI	TCATGA	3 cut(s)	112, 595, 1267
BspLI	GGNNCC	3 cut(s)	67, 887, 1339
BspOI	GCTAGC	1 cut(s)	522
BspPI	GGATC	1 cut(s)	562
BspT104I	TTCGAA	1 cut(s)	356
BspTNI	GGTCTC	1 cut(s)	788
BsrBI	CCGCTC	1 cut(s)	165
BsrFI	RCCGGY	5 cut(s)	193, 218, 285, 529, 797
BsrI	ACTGG	5 cut(s)	322, 760, 1043, 1180, 1201
BssAI	RCCGGY	5 cut(s)	193, 218, 285, 529, 797
BssECI	CCNNGG	3 cut(s)	308, 928, 1019
BssMI	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
BssSI	CACGAG	1 cut(s)	1385
BssT1I	CCWWGG	2 cut(s)	308, 1019
Bst2BI	CACGAG	1 cut(s)	1385
Bst2UI	CCWGG	1 cut(s)	1362
Bst4CI	ACNGT	4 cut(s)	568, 778, 1289, 1342
BstBI	TTCGAA	1 cut(s)	356
BstC8I	GCNNGC	3 cut(s)	216, 220, 520
BstDEI	CTNAG	2 cut(s)	132, 649
BstEII	GGTNACC	2 cut(s)	189, 1234
BstENI	CCTNNNNNAGG	1 cut(s)	836
BstF5I	GGATG	5 cut(s)	431, 637, 1059, 1087, 1297
BstH2I	RGCGCY	1 cut(s)	39
BstHHI	GCGC	2 cut(s)	38, 1137
BstKTI	GATC	9 cut(s)	112, 143, 557, 721, 1033, 1099, 1210, 1231, 1264
BstMAI	GTCTC	2 cut(s)	788, 1278
BstMBI	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
BstMCI	CGRYCG	1 cut(s)	223
BstMWI	GCNNNNNNNGC	2 cut(s)	377, 1250
BstNI	CCWGG	1 cut(s)	1362
BstPI	GGTNACC	2 cut(s)	189, 1234
BstSCI	CCNGG	2 cut(s)	927, 1360
BstSFI	CTRYAG	1 cut(s)	1288
BstV1I	GCAGC	2 cut(s)	673, 842
BstX2I	RGATCY	1 cut(s)	554
BstYI	RGATCY	1 cut(s)	554
BstZI	CGGCCG	1 cut(s)	220
BsuI	GTATCC	2 cut(s)	106, 1317
BsuRI	GGCC	6 cut(s)	218, 222, 396, 801, 1122, 1393
BtgZI	GCGATG	1 cut(s)	639
BtsCI	GGATG	5 cut(s)	431, 637, 1059, 1087, 1297
BtsI	GCAGTG	1 cut(s)	826
BtsIMutI	CAGTG	3 cut(s)	774, 826, 1194
Cac8I	GCNNGC	3 cut(s)	216, 220, 520
CaiI	CAGNNNCTG	1 cut(s)	855
CciI	TCATGA	3 cut(s)	112, 595, 1267
CfoI	GCGC	2 cut(s)	38, 1137
Cfr10I	RCCGGY	5 cut(s)	193, 218, 285, 529, 797
Cfr13I	GGNCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
CseI	GACGC	1 cut(s)	861
Csp6I	GTAC	5 cut(s)	868, 1015, 1060, 1173, 1186
CspAI	ACCGGT	2 cut(s)	193, 529
CviAII	CATG	8 cut(s)	113, 275, 523, 596, 1012, 1070, 1124, 1268
CviQI	GTAC	5 cut(s)	868, 1015, 1060, 1173, 1186
DdeI	CTNAG	2 cut(s)	132, 649
DpnI	GATC	9 cut(s)	111, 142, 556, 720, 1032, 1098, 1209, 1230, 1263
DpnII	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
DraIII	CACNNNGTG	1 cut(s)	780
DrdI	GACNNNNNNGTC	2 cut(s)	568, 1340
DseDI	GACNNNNNNGTC	2 cut(s)	568, 1340
EaeI	YGGCCR	3 cut(s)	216, 220, 799
EagI	CGGCCG	1 cut(s)	220
Ecl136II	GAGCTC	1 cut(s)	1244
EclXI	CGGCCG	1 cut(s)	220
Eco130I	CCWWGG	2 cut(s)	308, 1019
Eco24I	GRGCYC	1 cut(s)	1246
Eco31I	GGTCTC	1 cut(s)	788
Eco32I	GATATC	1 cut(s)	445
Eco47I	GGWCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
Eco52I	CGGCCG	1 cut(s)	220
Eco53kI	GAGCTC	1 cut(s)	1244
Eco57I	CTGAAG	1 cut(s)	81
Eco91I	GGTNACC	2 cut(s)	189, 1234
EcoICRI	GAGCTC	1 cut(s)	1244
EcoNI	CCTNNNNNAGG	1 cut(s)	836
EcoO109I	RGGNCCY	3 cut(s)	83, 886, 1274
EcoO65I	GGTNACC	2 cut(s)	189, 1234
EcoRII	CCWGG	1 cut(s)	1360
EcoRV	GATATC	1 cut(s)	445
EcoT14I	CCWWGG	2 cut(s)	308, 1019
EcoT38I	GRGCYC	1 cut(s)	1246
ErhI	CCWWGG	2 cut(s)	308, 1019
FaeI	CATG	8 cut(s)	116, 278, 526, 599, 1015, 1073, 1127, 1271
FalI	AAGNNNNNCTT	2 cut(s)	322, 354
FaqI	GGGAC	4 cut(s)	860, 909, 1346, 1351
FatI	CATG	8 cut(s)	112, 274, 522, 595, 1011, 1069, 1123, 1267
FauI	CCCGC	2 cut(s)	158, 237
FbaI	TGATCA	3 cut(s)	109, 1096, 1207
Fnu4HI	GCNGC	2 cut(s)	687, 856
FokI	GGATG	5 cut(s)	438, 644, 1066, 1074, 1284
FriOI	GRGCYC	1 cut(s)	1246
FseI	GGCCGGCC	1 cut(s)	222
Fsp4HI	GCNGC	2 cut(s)	687, 856
FspBI	CTAG	3 cut(s)	519, 1005, 1259
FspI	TGCGCA	1 cut(s)	1136
GlaI	GCGC	2 cut(s)	37, 1136
GluI	GCNGC	2 cut(s)	687, 856
GsaI	CCCAGC	2 cut(s)	180, 1067
GsuI	CTGGAG	1 cut(s)	1146
HaeII	RGCGCY	1 cut(s)	39
HaeIII	GGCC	6 cut(s)	218, 222, 396, 801, 1122, 1393
HgaI	GACGC	1 cut(s)	861
HhaI	GCGC	2 cut(s)	38, 1137
Hin1II	CATG	8 cut(s)	116, 278, 526, 599, 1015, 1073, 1127, 1271
Hin6I	GCGC	2 cut(s)	36, 1135
HinP1I	GCGC	2 cut(s)	36, 1135
HinfI	GANTC	1 cut(s)	252
HphI	GGTGA	3 cut(s)	483, 749, 1376
Hpy166II	GTNNAC	2 cut(s)	319, 772
Hpy188I	TCNGA	4 cut(s)	438, 746, 766, 1083
Hpy8I	GTNNAC	2 cut(s)	319, 772
Hpy99I	CGWCG	3 cut(s)	565, 568, 1346
HpyAV	CCTTC	8 cut(s)	56, 424, 433, 593, 614, 899, 955, 1265
HpyCH4III	ACNGT	4 cut(s)	568, 778, 1289, 1342
HpyCH4IV	ACGT	2 cut(s)	502, 866
HpyCH4V	TGCA	4 cut(s)	578, 689, 1073, 1127
HpyF10VI	GCNNNNNNNGC	2 cut(s)	377, 1250
HpyF3I	CTNAG	2 cut(s)	132, 649
HpySE526I	ACGT	2 cut(s)	502, 866
Hsp92II	CATG	8 cut(s)	116, 278, 526, 599, 1015, 1073, 1127, 1271
HspAI	GCGC	2 cut(s)	36, 1135
KroI	GCCGGC	1 cut(s)	218
KroNI	GCCGGC	1 cut(s)	220
Ksp22I	TGATCA	3 cut(s)	109, 1096, 1207
Kzo9I	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
LmnI	GCTCC	1 cut(s)	1241
Lsp1109I	GCAGC	2 cut(s)	673, 842
LweI	GCATC	3 cut(s)	688, 887, 1306
MaeI	CTAG	3 cut(s)	519, 1005, 1259
MaeII	ACGT	2 cut(s)	502, 866
MaeIII	GTNAC	8 cut(s)	5, 189, 821, 910, 941, 1034, 1151, 1234
MalI	GATC	9 cut(s)	111, 142, 556, 720, 1032, 1098, 1209, 1230, 1263
MbiI	CCGCTC	1 cut(s)	165
MboI	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
MboII	GAAGA	2 cut(s)	261, 549
MflI	RGATCY	1 cut(s)	554
MhlI	GDGCHC	1 cut(s)	1246
MluCI	AATT	9 cut(s)	52, 117, 232, 350, 635, 642, 656, 1143, 1355
MroNI	GCCGGC	1 cut(s)	218
MseI	TTAA	4 cut(s)	654, 860, 947, 1142
MslI	CAYNNNNRTG	2 cut(s)	1092, 1385
MspR9I	CCNGG	2 cut(s)	929, 1362
MvaI	CCWGG	1 cut(s)	1362
MwoI	GCNNNNNNNGC	2 cut(s)	377, 1250
NaeI	GCCGGC	1 cut(s)	220
NciI	CCSGG	1 cut(s)	929
NdeII	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
NgoMIV	GCCGGC	1 cut(s)	218
NheI	GCTAGC	1 cut(s)	518
NlaIII	CATG	8 cut(s)	116, 278, 526, 599, 1015, 1073, 1127, 1271
NlaIV	GGNNCC	3 cut(s)	67, 887, 1339
NmuCI	GTSAC	2 cut(s)	821, 1034
NsbI	TGCGCA	1 cut(s)	1136
NspV	TTCGAA	1 cut(s)	356
OliI	CACNNNNGTG	1 cut(s)	1385
PagI	TCATGA	3 cut(s)	112, 595, 1267
PdiI	GCCGGC	1 cut(s)	220
PfeI	GAWTC	1 cut(s)	252
Pfl23II	CGTACG	1 cut(s)	867
PflFI	GACNNNGTC	1 cut(s)	568
PflMI	CCANNNNNTGG	1 cut(s)	981
PinAI	ACCGGT	2 cut(s)	193, 529
PkrI	GCNGC	2 cut(s)	688, 857
PpuMI	RGGWCCY	3 cut(s)	83, 886, 1274
Psp124BI	GAGCTC	1 cut(s)	1246
Psp1406I	AACGTT	1 cut(s)	502
Psp5II	RGGWCCY	3 cut(s)	83, 886, 1274
Psp6I	CCWGG	1 cut(s)	1360
PspEI	GGTNACC	2 cut(s)	189, 1234
PspFI	CCCAGC	2 cut(s)	176, 1063
PspGI	CCWGG	1 cut(s)	1360
PspLI	CGTACG	1 cut(s)	867
PspN4I	GGNNCC	3 cut(s)	67, 887, 1339
PspPI	GGNCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
PspPPI	RGGWCCY	3 cut(s)	83, 886, 1274
PstNI	CAGNNNCTG	1 cut(s)	855
PsuI	RGATCY	1 cut(s)	554
PsyI	GACNNNGTC	1 cut(s)	568
RigI	GGCCGGCC	1 cut(s)	222
RsaI	GTAC	5 cut(s)	869, 1016, 1061, 1174, 1187
RsaNI	GTAC	5 cut(s)	868, 1015, 1060, 1173, 1186
RseI	CAYNNNNRTG	2 cut(s)	1092, 1385
SacI	GAGCTC	1 cut(s)	1246
SaqAI	TTAA	4 cut(s)	654, 860, 947, 1142
SatI	GCNGC	2 cut(s)	687, 856
Sau3AI	GATC	9 cut(s)	109, 140, 554, 718, 1030, 1096, 1207, 1228, 1261
Sau96I	GGNCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
ScaI	AGTACT	1 cut(s)	1174
ScrFI	CCNGG	2 cut(s)	929, 1362
SduI	GDGCHC	1 cut(s)	1246
SfaNI	GCATC	3 cut(s)	688, 887, 1306
SfcI	CTRYAG	1 cut(s)	1288
SfuI	TTCGAA	1 cut(s)	356
SinI	GGWCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
SmiMI	CAYNNNNRTG	2 cut(s)	1092, 1385
SmlI	CTYRAG	3 cut(s)	460, 1201, 1245
SmoI	CTYRAG	3 cut(s)	460, 1201, 1245
Sse9I	AATT	9 cut(s)	52, 117, 232, 350, 635, 642, 656, 1143, 1355
SsiI	CCGC	3 cut(s)	48, 165, 244
SspI	AATATT	1 cut(s)	541
SspMI	CTAG	3 cut(s)	519, 1005, 1259
SstI	GAGCTC	1 cut(s)	1246
StyD4I	CCNGG	2 cut(s)	927, 1360
StyI	CCWWGG	2 cut(s)	308, 1019
TaaI	ACNGT	4 cut(s)	568, 778, 1289, 1342
TaiI	ACGT	2 cut(s)	505, 869
TaqI	TCGA	3 cut(s)	185, 356, 560
TasI	AATT	9 cut(s)	52, 117, 232, 350, 635, 642, 656, 1143, 1355
TatI	WGTACW	2 cut(s)	1172, 1185
TfiI	GAWTC	1 cut(s)	252
Tru1I	TTAA	4 cut(s)	654, 860, 947, 1142
Tru9I	TTAA	4 cut(s)	654, 860, 947, 1142
TscAI	CASTG	3 cut(s)	781, 826, 1201
TseFI	GTSAC	2 cut(s)	821, 1034
TseI	GCWGC	2 cut(s)	686, 855
Tsp45I	GTSAC	2 cut(s)	821, 1034
TspDTI	ATGAA	7 cut(s)	105, 129, 221, 584, 612, 648, 1284
TspGWI	ACGGA	2 cut(s)	885, 1204
TspRI	CASTG	3 cut(s)	781, 826, 1201
Tth111I	GACNNNGTC	1 cut(s)	568
Van91I	CCANNNNNTGG	1 cut(s)	981
VpaK11BI	GGWCC	8 cut(s)	83, 305, 319, 496, 886, 964, 1274, 1338
XagI	CCTNNNNNAGG	1 cut(s)	836
XapI	RAATTY	5 cut(s)	52, 635, 642, 656, 1355
XbaI	TCTAGA	1 cut(s)	1004
XspI	CTAG	3 cut(s)	519, 1005, 1259
ZrmI	AGTACT	1 cut(s)	1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.