Rroxscaffold_5G00363080

LEC14B homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001751
Physical Location & Seq
Reverse (-)
44176009 .. 44182730
6722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00363080.1

Sequence Viewer

Length: 1113 bp
ATGGGGGTGGACCAGTTGAATAGCCGTGCATATGTTTCTCAGTTTTCGAATGATGGCTTGTTTTTTGTTGCTGGATTTCAGGGAGGTCATATTAGAATATATAATGTTGAAAAGGGATGGACAGTTCAGAAGGACATTCTAACCAAAAGCTTGAGTTGGACAATTACTGATACATCTCTATCACCTGATCAACGATTTCTTGCATATGCTAGCATGTCACCTATTGTCCATGTTGTCAATGCTGGATCTTCTACGACAGAGTCACTTGCAAACATTACGGAAATACATGAAGATCTGGATTTTTCTGTTGATTATGATGAAGAATTTGGAATATTCTCTGTTAAATTTTCAACTGATGGGCGAGAGCTTGTGGCTGGAAGTAGTGATGCTTCAATACATGTTTATGATCTCCAAGCAAACAAAGTTAGCCTCAACATACCAGCGCATACGTCAGATGTAAACACAGTATGCTTTGCAGATGAGACTGGCCATCTCATATTTTCCGGCGGTGATGATAACCTCTGTAAGGTGTGGGATAGACGCTGCTTTATCACGAAAGGGCAAGCAGCTGGGGTTCTGATGGGACATCTTCAAGGCATAACGTTTATTGATAGTCGGGGAGATGGACGTTACTTAATATCAAATGGGAAGGATCAGACAACCAAACTTTGGGATATACGGAAGATGTCCTCTAGAAATATGTATCCAAGGATCACAGATTGTGACTGGGATTACAGGTGGATGGAGTACCCAGCTCATGTAAGAACTTCGAGACATCCCTTGGATCAGTCGTTGGCTACATATAGAGACCATTCAGTTCTGCGCACTTTAATTCGCTGTTACTTTTCTCCAGCATATAGCACTGGACAAAAGTACATCTACACTGGATCTAGTGATCATTCGGTCTATATTTATGATCTGGTGACTGGAGCTCAAGTTGCGAAACTCTTTCATCATGATGGACCTGTAAGAGATTGTAGTTGGCATCCTACATATCCCATGTTGGTCAGTTCTTCCTGGGATGGGAGTATCGTGAGATGGGAGTTTCCGGGAGATGATCAAGTTCCTACCCTGGTCAAGCCAAGAGTAAAGCGGAGGAGAACTTTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

370

Amino Acids

41.93

Weight (kDa)

6.15

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_RIG_2nd PF23775 6 - 170 1e-05 RIG second beta-propeller
Beta-prop_EML_2 PF23414 6 - 104 4.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 14 - 181 4.7e-16 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 24 - 178 1.4e-09 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 30 - 179 2.1e-12 WDR3 first beta-propeller domain
WDR55 PF24796 54 - 228 6.8e-15 WDR55
WD40_Prp19 PF24814 55 - 181 4.4e-11 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 76 - 179 3.2e-08 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 79 - 181 9.2e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 105 - 225 1.1e-13 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 106 - 235 9.7e-16 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 110 - 229 8.8e-19 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 111 - 179 3.1e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 111 - 245 9.6e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 111 - 181 6e-12 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 123 - 244 2.7e-08 TEP-1 second beta-propeller
WD40 PF00400 145 - 179 4.5e-06 WD domain, G-beta repeat
EIF3I PF24805 148 - 342 1.9e-10 EIF3I
WD40_WDHD1_1st PF24817 194 - 357 1.1e-11 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 271 - 347 1.2e-09 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 273 - 355 1e-05 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 285 - 350 7.6e-07 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 285 - 362 3e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 289 - 353 3.1e-08 Prp19 WD40 domain
WD40 PF00400 312 - 347 8.9e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 669
AciI CCGC 2 cut(s) 507, 1093
AclI AACGTT 1 cut(s) 602
AclWI GGATC 5 cut(s) 253, 660, 719, 792, 895
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 2 cut(s) 323, 344
AfaI GTAC 2 cut(s) 749, 875
AfiI CCNNNNNNNGG 3 cut(s) 526, 669, 1023
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 5 cut(s) 19, 110, 351, 393, 593
AjnI CCWGG 2 cut(s) 1016, 1071
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
AluBI AGCT 5 cut(s) 150, 367, 569, 755, 932
AluI AGCT 5 cut(s) 150, 367, 569, 755, 932
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 3 cut(s) 476, 766, 801
AlwI GGATC 5 cut(s) 253, 660, 719, 792, 895
AoxI GGCC 1 cut(s) 487
ApeKI GCWGC 2 cut(s) 543, 566
ApoI RAATTY 2 cut(s) 323, 344
AspLEI GCGC 2 cut(s) 445, 825
AspS9I GGNCC 2 cut(s) 10, 962
AsuC2I CCSGG 1 cut(s) 1050
AsuHPI GGTGA 4 cut(s) 174, 210, 521, 934
AsuII TTCGAA 1 cut(s) 47
AsuNHI GCTAGC 1 cut(s) 209
AvaII GGWCC 2 cut(s) 10, 962
BalI TGGCCA 1 cut(s) 489
BanII GRGCYC 1 cut(s) 934
BarI GAAGNNNNNNTAC 2 cut(s) 373, 405
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 2 cut(s) 530, 578
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 2 cut(s) 1018, 1073
BciVI GTATCC 1 cut(s) 714
BclI TGATCA 3 cut(s) 187, 895, 1057
BcnI CCSGG 1 cut(s) 1050
BcoDI GTCTC 3 cut(s) 476, 766, 801
BfaI CTAG 3 cut(s) 210, 693, 891
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 1 cut(s) 292
BisI GCNGC 2 cut(s) 544, 567
BlsI GCNGC 2 cut(s) 545, 568
Bme1390I CCNGG 3 cut(s) 1018, 1050, 1073
Bme18I GGWCC 2 cut(s) 10, 962
BmgT120I GGNCC 2 cut(s) 10, 962
BmrFI CCNGG 3 cut(s) 1018, 1050, 1073
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 2 cut(s) 376, 994
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 736
BpmI CTGGAG 2 cut(s) 834, 948
Bpu14I TTCGAA 1 cut(s) 47
BpuEI CTTGAG 2 cut(s) 172, 918
BpuMI CCSGG 1 cut(s) 1050
BsaI GGTCTC 1 cut(s) 801
BsaJI CCNNGG 4 cut(s) 707, 780, 1017, 1071
BsaXI ACNNNNNCTCC 2 cut(s) 921, 951
Bsc4I CCNNNNNNNGG 3 cut(s) 526, 669, 1023
Bse1I ACTGG 6 cut(s) 13, 490, 731, 868, 889, 931
BseBI CCWGG 2 cut(s) 1018, 1073
BseDI CCNNGG 4 cut(s) 707, 780, 1017, 1071
BseGI GGATG 5 cut(s) 122, 747, 775, 985, 1027
BseLI CCNNNNNNNGG 3 cut(s) 526, 669, 1023
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 6 cut(s) 13, 490, 731, 868, 889, 931
BseRI GAGGAG 1 cut(s) 1111
BseXI GCAGC 2 cut(s) 530, 578
BseYI CCCAGC 2 cut(s) 569, 751
BshFI GGCC 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 934
BsiSI CCGG 2 cut(s) 504, 1049
BslFI GGGAC 1 cut(s) 597
BslI CCNNNNNNNGG 3 cut(s) 526, 669, 1023
BsmAI GTCTC 3 cut(s) 476, 766, 801
BsmFI GGGAC 1 cut(s) 597
BsnI GGCC 1 cut(s) 489
Bso31I GGTCTC 1 cut(s) 801
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 934
BspACI CCGC 2 cut(s) 507, 1093
BspANI GGCC 1 cut(s) 489
BspCNI CTCAG 1 cut(s) 52
BspHI TCATGA 1 cut(s) 955
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 5 cut(s) 253, 660, 719, 792, 895
BspT104I TTCGAA 1 cut(s) 47
BspTNI GGTCTC 1 cut(s) 801
BsrI ACTGG 6 cut(s) 13, 490, 731, 868, 889, 931
BssECI CCNNGG 4 cut(s) 707, 780, 1017, 1071
BssT1I CCWWGG 2 cut(s) 707, 780
Bst2UI CCWGG 2 cut(s) 1018, 1073
Bst4CI ACNGT 2 cut(s) 124, 466
BstBI TTCGAA 1 cut(s) 47
BstC8I GCNNGC 2 cut(s) 211, 564
BstDEI CTNAG 1 cut(s) 39
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 5 cut(s) 122, 747, 775, 985, 1027
BstHHI GCGC 2 cut(s) 445, 825
BstMAI GTCTC 3 cut(s) 476, 766, 801
BstMWI GCNNNNNNNGC 1 cut(s) 938
BstNI CCWGG 2 cut(s) 1018, 1073
BstNSI RCATGY 2 cut(s) 217, 401
BstSCI CCNGG 3 cut(s) 1016, 1048, 1071
BstV1I GCAGC 2 cut(s) 530, 578
BstX2I RGATCY 3 cut(s) 245, 292, 887
BstYI RGATCY 3 cut(s) 245, 292, 887
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 1 cut(s) 489
BtsCI GGATG 5 cut(s) 122, 747, 775, 985, 1027
BtsIMutI CAGTG 2 cut(s) 861, 882
Cac8I GCNNGC 2 cut(s) 211, 564
CciI TCATGA 1 cut(s) 955
CfoI GCGC 2 cut(s) 445, 825
Cfr13I GGNCC 2 cut(s) 10, 962
CseI GACGC 1 cut(s) 549
Csp6I GTAC 2 cut(s) 748, 874
CviAII CATG 7 cut(s) 214, 230, 287, 398, 758, 956, 1000
CviQI GTAC 2 cut(s) 748, 874
DdeI CTNAG 1 cut(s) 39
EaeI YGGCCR 1 cut(s) 487
Ecl136II GAGCTC 1 cut(s) 932
Eco130I CCWWGG 2 cut(s) 707, 780
Eco24I GRGCYC 1 cut(s) 934
Eco31I GGTCTC 1 cut(s) 801
Eco47I GGWCC 2 cut(s) 10, 962
Eco53kI GAGCTC 1 cut(s) 932
EcoICRI GAGCTC 1 cut(s) 932
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 1016, 1071
EcoT14I CCWWGG 2 cut(s) 707, 780
EcoT38I GRGCYC 1 cut(s) 934
ErhI CCWWGG 2 cut(s) 707, 780
FaeI CATG 7 cut(s) 217, 233, 290, 401, 761, 959, 1003
FaqI GGGAC 1 cut(s) 597
FatI CATG 7 cut(s) 213, 229, 286, 397, 757, 955, 999
FauNDI CATATG 2 cut(s) 31, 205
FbaI TGATCA 3 cut(s) 187, 895, 1057
Fnu4HI GCNGC 2 cut(s) 544, 567
FokI GGATG 5 cut(s) 129, 754, 762, 972, 1034
FriOI GRGCYC 1 cut(s) 934
Fsp4HI GCNGC 2 cut(s) 544, 567
FspBI CTAG 3 cut(s) 210, 693, 891
FspI TGCGCA 1 cut(s) 824
GlaI GCGC 2 cut(s) 444, 824
GluI GCNGC 2 cut(s) 544, 567
GsaI CCCAGC 2 cut(s) 573, 755
GsuI CTGGAG 2 cut(s) 834, 948
HaeIII GGCC 1 cut(s) 489
HapII CCGG 2 cut(s) 504, 1049
HgaI GACGC 1 cut(s) 549
HhaI GCGC 2 cut(s) 445, 825
Hin1II CATG 7 cut(s) 217, 233, 290, 401, 761, 959, 1003
Hin6I GCGC 2 cut(s) 443, 823
HinP1I GCGC 2 cut(s) 443, 823
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 1 cut(s) 260
HpaII CCGG 2 cut(s) 504, 1049
HphI GGTGA 4 cut(s) 174, 210, 521, 934
Hpy166II GTNNAC 2 cut(s) 10, 460
Hpy188I TCNGA 4 cut(s) 129, 454, 579, 657
Hpy188III TCNNGA 6 cut(s) 296, 553, 693, 771, 956, 1033
Hpy8I GTNNAC 2 cut(s) 10, 460
HpyAV CCTTC 2 cut(s) 124, 643
HpyCH4III ACNGT 2 cut(s) 124, 466
HpyCH4IV ACGT 3 cut(s) 449, 602, 628
HpyCH4V TGCA 4 cut(s) 29, 203, 269, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 938
HpyF3I CTNAG 1 cut(s) 39
HpySE526I ACGT 3 cut(s) 449, 602, 628
Hsp92II CATG 7 cut(s) 217, 233, 290, 401, 761, 959, 1003
HspAI GCGC 2 cut(s) 443, 823
Ksp22I TGATCA 3 cut(s) 187, 895, 1057
LmnI GCTCC 1 cut(s) 929
Lsp1109I GCAGC 2 cut(s) 530, 578
LweI GCATC 2 cut(s) 376, 994
MaeI CTAG 3 cut(s) 210, 693, 891
MaeII ACGT 3 cut(s) 449, 602, 628
MaeIII GTNAC 6 cut(s) 216, 261, 629, 722, 839, 922
MboII GAAGA 6 cut(s) 240, 302, 332, 581, 694, 1005
MflI RGATCY 3 cut(s) 245, 292, 887
MhlI GDGCHC 1 cut(s) 934
MlsI TGGCCA 1 cut(s) 489
MluCI AATT 4 cut(s) 162, 323, 344, 831
MluNI TGGCCA 1 cut(s) 489
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 5 cut(s) 77, 440, 530, 700, 1089
Mox20I TGGCCA 1 cut(s) 489
MscI TGGCCA 1 cut(s) 489
MseI TTAA 3 cut(s) 342, 635, 830
MslI CAYNNNNRTG 2 cut(s) 402, 957
Msp20I TGGCCA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 2 cut(s) 504, 1049
MspR9I CCNGG 3 cut(s) 1018, 1050, 1073
MvaI CCWGG 2 cut(s) 1018, 1073
MwoI GCNNNNNNNGC 1 cut(s) 938
NciI CCSGG 1 cut(s) 1050
NdeI CATATG 2 cut(s) 31, 205
NheI GCTAGC 1 cut(s) 209
NlaIII CATG 7 cut(s) 217, 233, 290, 401, 761, 959, 1003
NmuCI GTSAC 4 cut(s) 216, 261, 722, 922
NsbI TGCGCA 1 cut(s) 824
NspI RCATGY 2 cut(s) 217, 401
NspV TTCGAA 1 cut(s) 47
PagI TCATGA 1 cut(s) 955
PciI ACATGT 1 cut(s) 397
PflFI GACNNNGTC 1 cut(s) 259
PflMI CCANNNNNTGG 1 cut(s) 669
PfoI TCCNGGA 1 cut(s) 1048
PkrI GCNGC 2 cut(s) 545, 568
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PscI ACATGT 1 cut(s) 397
Psp124BI GAGCTC 1 cut(s) 934
Psp1406I AACGTT 1 cut(s) 602
Psp6I CCWGG 2 cut(s) 1016, 1071
PspFI CCCAGC 2 cut(s) 569, 751
PspGI CCWGG 2 cut(s) 1016, 1071
PspPI GGNCC 2 cut(s) 10, 962
PsuI RGATCY 3 cut(s) 245, 292, 887
PsyI GACNNNGTC 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 2 cut(s) 749, 875
RsaNI GTAC 2 cut(s) 748, 874
RseI CAYNNNNRTG 2 cut(s) 402, 957
SacI GAGCTC 1 cut(s) 934
SaqAI TTAA 3 cut(s) 342, 635, 830
SatI GCNGC 2 cut(s) 544, 567
Sau96I GGNCC 2 cut(s) 10, 962
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 3 cut(s) 1018, 1050, 1073
SduI GDGCHC 1 cut(s) 934
SfaNI GCATC 2 cut(s) 376, 994
SfuI TTCGAA 1 cut(s) 47
SinI GGWCC 2 cut(s) 10, 962
SmiMI CAYNNNNRTG 2 cut(s) 402, 957
SmlI CTYRAG 2 cut(s) 151, 933
SmoI CTYRAG 2 cut(s) 151, 933
Sse9I AATT 4 cut(s) 162, 323, 344, 831
SsiI CCGC 2 cut(s) 507, 1093
SspI AATATT 1 cut(s) 333
SspMI CTAG 3 cut(s) 210, 693, 891
SstI GAGCTC 1 cut(s) 934
StyD4I CCNGG 3 cut(s) 1016, 1048, 1071
StyI CCWWGG 2 cut(s) 707, 780
TaaI ACNGT 2 cut(s) 124, 466
TaiI ACGT 3 cut(s) 452, 605, 631
TaqI TCGA 2 cut(s) 47, 770
TaqII GACCGA 1 cut(s) 892
TasI AATT 4 cut(s) 162, 323, 344, 831
TatI WGTACW 1 cut(s) 873
Tru1I TTAA 3 cut(s) 342, 635, 830
Tru9I TTAA 3 cut(s) 342, 635, 830
TscAI CASTG 2 cut(s) 868, 889
TseFI GTSAC 4 cut(s) 216, 261, 722, 922
TseI GCWGC 2 cut(s) 543, 566
Tsp45I GTSAC 4 cut(s) 216, 261, 722, 922
TspDTI ATGAA 3 cut(s) 303, 333, 941
TspGWI ACGGA 2 cut(s) 293, 694
TspRI CASTG 2 cut(s) 868, 889
Tth111I GACNNNGTC 1 cut(s) 259
Van91I CCANNNNNTGG 1 cut(s) 669
VpaK11BI GGWCC 2 cut(s) 10, 962
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 2 cut(s) 323, 344
XbaI TCTAGA 1 cut(s) 692
XceI RCATGY 2 cut(s) 217, 401
XspI CTAG 3 cut(s) 210, 693, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.