pycom03g21760

LEC14B protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001751
Physical Location & Seq
Reverse (-)
21997219 .. 22000359
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21760.6

Sequence Viewer

Length: 1413 bp
ATGGGGTATTCACTCAGTAGATTGACACTAGAATCTGATCATTTTGATCCTGGAAATGCTGCCGTCCATGGAGTCGGTAACGGTCAAACACCCAACAAACCATTGCATAATCTAGAGCATGAAGTAGCCCAGCTCACAAAGTTGAGGTCAGCTCCCAATGAGCGGCTTACCCAAATTGGACCTAGAAGGCGGGACTTGCCCGTTTCCACGGTCAGGATGCTGGCTGGTCGAGAGGGCAATTATTCGGGAAGAGGAGGAAGGTTCTCTTCTGCAGATCGTTGCCATGTTCTAAGTAGATATTTGCCAGTCAATGGTCCTTGGTTTGTCGACCGAAGGTCTAGCCGAGCTTATGTCTCTCAGTTTTCGGCAGATGGATCCCTTTTCATTGCTGGGTTTCAGGATGGTCATATTAAAATATACAATGTGGATAGAGAGTGGAAAGTCCAGAAGAACATTCATGTCCAAAGTATGCGATGGACTGTCACTGATACGTCTCTTTCCCCGGATCAACGCCATCTTGTTTATGCGAGTATGTCCCCTATTGTTCATATGGTTGATGTGGGATCTTCTGAAGTGGAGTCTCGTGCAAATATAACGGAGATCCACGAGGGTTTGGATTTTTCCTCTGGTGATGACGGAGGGTACTCATTTGGTATTTTCTCCATAAAATTCTCAACAGATGGACATGAACTTGTTGCTGGAAGTAGCGATGATTCCATATATGTTTATGATCTTGAAGCCAATAAGCTCTCCCTTCGGATTCCCGCACACTCGTCTGATGTGAACACCGTATGCTTTGCTGATGAAAGTGGCCATCTTATTTATTCTGGGAGTGATGATACTCTCTGTAAGGTATGGGATAGACGTTGCTTTAACACCACAAGGAAGCCAGCAGGAGTCTTGACTGGACACCTAGAAGGTATTGCATTCATTGACAGCAAGGGAGATGGTCGTTATTTCATTTCGAATGGTAAAGATCAGACCATAAAACTTTGGGATATCAGAAAAATGTCTTCTAGTCCCGCTCGCACTCCAGGGCTCAGGAGTTCTGAGTGGGATTACAGATGGATGGACTACCCACCCCAGGCAAAAGATATGAAACATCCGTCTGATCAATCAGTAGCTACGTATAGAGGACATTCAGTCTTGCGTACGCTTATTCGCTGCTACTTTTCTCCGGCATATAGCACCGGTCAGAAGTACATATACACGGGATCGCATAACTGTTGTGTGTATATATATGATTTGGTTACCGGAGCCCAAGTTGCGACGCTCAAGTATCATAAAGCACCGGTGAGGGACTGTAGTTGGCACCCCTATTACCCGATGCTCGTAAGCTCTTCTTGGGACGGGACCGTTGCTAAATGGGAGTTCCCCGGCAGTGGAGAAGCGCCGGCCTCGTTGTATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

471

Amino Acids

52.61

Weight (kDa)

7.3

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1311
AccB7I CCANNNNNTGG 1 cut(s) 311
AccBSI CCGCTC 2 cut(s) 163, 1025
AccI GTMKAC 1 cut(s) 327
AciI CCGC 4 cut(s) 163, 190, 765, 1023
AclWI GGATC 7 cut(s) 41, 369, 382, 513, 571, 595, 1222
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 3 cut(s) 644, 1153, 1202
AfiI CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
AgeI ACCGGT 2 cut(s) 1190, 1291
AgsI TTSAA 1 cut(s) 737
AhdI GACNNNNNGTC 2 cut(s) 334, 1142
AjnI CCWGG 3 cut(s) 49, 1033, 1083
AluBI AGCT 6 cut(s) 133, 152, 347, 748, 1124, 1338
AluI AGCT 6 cut(s) 133, 152, 347, 748, 1124, 1338
Alw26I GTCTC 3 cut(s) 358, 498, 585
AlwI GGATC 7 cut(s) 41, 369, 382, 513, 571, 595, 1222
AoxI GGCC 2 cut(s) 811, 1395
ApeKI GCWGC 2 cut(s) 59, 1164
ApoI RAATTY 1 cut(s) 668
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AsiGI ACCGGT 2 cut(s) 1190, 1291
AspLEI GCGC 1 cut(s) 1393
AspS9I GGNCC 3 cut(s) 179, 314, 1353
AsuC2I CCSGG 2 cut(s) 503, 1377
AsuHPI GGTGA 2 cut(s) 641, 1306
AsuII TTCGAA 1 cut(s) 965
AvaII GGWCC 3 cut(s) 179, 314, 1353
BalI TGGCCA 1 cut(s) 813
BamHI GGATCC 1 cut(s) 374
BanI GGYRCC 1 cut(s) 1311
BanII GRGCYC 2 cut(s) 1041, 1261
BarI GAAGNNNNNNTAC 2 cut(s) 1190, 1222
BauI CACGAG 2 cut(s) 582, 605
BbsI GAAGAC 1 cut(s) 1005
BbvI GCAGC 2 cut(s) 46, 1151
BccI CCATC 9 cut(s) 365, 395, 468, 522, 674, 822, 941, 1059, 1063
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 3 cut(s) 51, 1035, 1085
BclI TGATCA 2 cut(s) 37, 1111
BcnI CCSGG 2 cut(s) 503, 1377
BcoDI GTCTC 3 cut(s) 358, 498, 585
BfaI CTAG 6 cut(s) 29, 113, 183, 339, 914, 1017
BfmI CTRYAG 2 cut(s) 270, 1303
BfoI RGCGCY 1 cut(s) 1394
BisI GCNGC 3 cut(s) 60, 164, 1165
BlsI GCNGC 3 cut(s) 61, 165, 1166
Bme1390I CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
Bme18I GGWCC 3 cut(s) 179, 314, 1353
BmeRI GACNNNNNGTC 2 cut(s) 334, 1142
BmgT120I GGNCC 3 cut(s) 179, 314, 1353
BmiI GGNNCC 4 cut(s) 376, 1258, 1313, 1354
BmrFI CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
BmsI GCATC 2 cut(s) 207, 1317
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 1 cut(s) 1017
Bpu10I CCTNAGC 1 cut(s) 1040
Bpu14I TTCGAA 1 cut(s) 965
BpuEI CTTGAG 1 cut(s) 1259
BpuMI CCSGG 2 cut(s) 503, 1377
BsaAI YACGTR 1 cut(s) 1128
BsaJI CCNNGG 7 cut(s) 67, 207, 317, 501, 1034, 1083, 1375
BsaWI WCCGGW 3 cut(s) 1190, 1253, 1291
BsaXI ACNNNNNCTCC 2 cut(s) 1248, 1278
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
Bse118I RCCGGY 3 cut(s) 1190, 1291, 1393
Bse1I ACTGG 2 cut(s) 305, 910
Bse3DI GCAATG 2 cut(s) 101, 384
BseBI CCWGG 3 cut(s) 51, 1035, 1085
BseDI CCNNGG 7 cut(s) 67, 207, 317, 501, 1034, 1083, 1375
BseGI GGATG 4 cut(s) 222, 406, 1074, 1102
BseLI CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
BseMI GCAATG 2 cut(s) 101, 384
BseMII CTCAG 4 cut(s) 28, 371, 1041, 1054
BseNI ACTGG 2 cut(s) 305, 910
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 2 cut(s) 46, 1151
BseYI CCCAGC 2 cut(s) 129, 389
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 2 cut(s) 813, 1397
BshNI GGYRCC 1 cut(s) 1311
BshTI ACCGGT 2 cut(s) 1190, 1291
BsiEI CGRYCG 1 cut(s) 331
BsiSI CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
BsiWI CGTACG 1 cut(s) 1151
BslFI GGGAC 6 cut(s) 206, 520, 1005, 1313, 1361, 1366
BslI CCNNNNNNNGG 5 cut(s) 162, 213, 311, 1084, 1382
BsmAI GTCTC 3 cut(s) 358, 498, 585
BsmBI CGTCTC 1 cut(s) 498
BsmFI GGGAC 6 cut(s) 206, 520, 1005, 1313, 1361, 1366
BsmI GAATGC 1 cut(s) 926
BsnI GGCC 2 cut(s) 813, 1397
Bsp119I TTCGAA 1 cut(s) 965
Bsp1286I GDGCHC 2 cut(s) 1041, 1261
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 4 cut(s) 163, 190, 765, 1023
BspANI GGCC 2 cut(s) 813, 1397
BspCNI CTCAG 4 cut(s) 27, 370, 1042, 1053
BspLI GGNNCC 4 cut(s) 376, 1258, 1313, 1354
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 7 cut(s) 41, 369, 382, 513, 571, 595, 1222
BspQI GCTCTTC 1 cut(s) 1345
BspT104I TTCGAA 1 cut(s) 965
BspT107I GGYRCC 1 cut(s) 1311
BsrBI CCGCTC 2 cut(s) 163, 1025
BsrDI GCAATG 2 cut(s) 101, 384
BsrFI RCCGGY 3 cut(s) 1190, 1291, 1393
BsrI ACTGG 2 cut(s) 305, 910
BssAI RCCGGY 3 cut(s) 1190, 1291, 1393
BssECI CCNNGG 7 cut(s) 67, 207, 317, 501, 1034, 1083, 1375
BssSI CACGAG 2 cut(s) 582, 605
BssT1I CCWWGG 2 cut(s) 67, 317
Bst2BI CACGAG 2 cut(s) 582, 605
Bst2UI CCWGG 3 cut(s) 51, 1035, 1085
Bst4CI ACNGT 7 cut(s) 83, 211, 481, 790, 1226, 1304, 1357
Bst6I CTCTTC 3 cut(s) 244, 271, 1345
BstBAI YACGTR 1 cut(s) 1128
BstBI TTCGAA 1 cut(s) 965
BstC8I GCNNGC 4 cut(s) 222, 891, 1027, 1395
BstDEI CTNAG 5 cut(s) 14, 290, 357, 1040, 1050
BstDSI CCRYGG 2 cut(s) 67, 207
BstEII GGTNACC 1 cut(s) 1249
BstF5I GGATG 4 cut(s) 222, 406, 1074, 1102
BstH2I RGCGCY 1 cut(s) 1394
BstHHI GCGC 1 cut(s) 1393
BstMAI GTCTC 3 cut(s) 358, 498, 585
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 196, 1265
BstNI CCWGG 3 cut(s) 51, 1035, 1085
BstPI GGTNACC 1 cut(s) 1249
BstSCI CCNGG 5 cut(s) 49, 501, 1033, 1083, 1375
BstSFI CTRYAG 2 cut(s) 270, 1303
BstSNI TACGTA 1 cut(s) 1128
BstV1I GCAGC 2 cut(s) 46, 1151
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 3 cut(s) 374, 563, 600
BstYI RGATCY 3 cut(s) 374, 563, 600
BsuRI GGCC 2 cut(s) 813, 1397
BtgI CCRYGG 2 cut(s) 67, 207
BtgZI GCGATG 2 cut(s) 487, 723
BtsCI GGATG 4 cut(s) 222, 406, 1074, 1102
BtsI GCAGTG 1 cut(s) 1387
BtsIMutI CAGTG 2 cut(s) 483, 1387
Cac8I GCNNGC 4 cut(s) 222, 891, 1027, 1395
CfoI GCGC 1 cut(s) 1393
Cfr10I RCCGGY 3 cut(s) 1190, 1291, 1393
Cfr13I GGNCC 3 cut(s) 179, 314, 1353
CseI GACGC 1 cut(s) 1279
Csp6I GTAC 3 cut(s) 643, 1152, 1201
CspAI ACCGGT 2 cut(s) 1190, 1291
CviAII CATG 5 cut(s) 68, 119, 284, 458, 686
CviQI GTAC 3 cut(s) 643, 1152, 1201
DdeI CTNAG 5 cut(s) 14, 290, 357, 1040, 1050
DriI GACNNNNNGTC 2 cut(s) 334, 1142
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 3 cut(s) 244, 271, 1345
Eam1105I GACNNNNNGTC 2 cut(s) 334, 1142
EarI CTCTTC 3 cut(s) 244, 271, 1345
Eco105I TACGTA 1 cut(s) 1128
Eco130I CCWWGG 2 cut(s) 67, 317
Eco24I GRGCYC 2 cut(s) 1041, 1261
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 3 cut(s) 179, 314, 1353
Eco57I CTGAAG 1 cut(s) 591
Eco91I GGTNACC 1 cut(s) 1249
EcoO65I GGTNACC 1 cut(s) 1249
EcoRII CCWGG 3 cut(s) 49, 1033, 1083
EcoRV GATATC 1 cut(s) 1000
EcoT14I CCWWGG 2 cut(s) 67, 317
EcoT38I GRGCYC 2 cut(s) 1041, 1261
ErhI CCWWGG 2 cut(s) 67, 317
Esp3I CGTCTC 1 cut(s) 498
FaeI CATG 5 cut(s) 71, 122, 287, 461, 689
FalI AAGNNNNNCTT 4 cut(s) 250, 282, 1327, 1359
FaqI GGGAC 6 cut(s) 206, 520, 1005, 1313, 1361, 1366
FatI CATG 5 cut(s) 67, 118, 283, 457, 685
FauI CCCGC 3 cut(s) 183, 772, 1030
FauNDI CATATG 1 cut(s) 549
FbaI TGATCA 2 cut(s) 37, 1111
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 3 cut(s) 60, 164, 1165
FokI GGATG 4 cut(s) 229, 413, 1081, 1089
FriOI GRGCYC 2 cut(s) 1041, 1261
Fsp4HI GCNGC 3 cut(s) 60, 164, 1165
FspBI CTAG 6 cut(s) 29, 113, 183, 339, 914, 1017
GlaI GCGC 1 cut(s) 1392
GluI GCNGC 3 cut(s) 60, 164, 1165
GsaI CCCAGC 2 cut(s) 133, 393
GsuI CTGGAG 1 cut(s) 1017
HaeII RGCGCY 1 cut(s) 1394
HaeIII GGCC 2 cut(s) 813, 1397
HapII CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
HgaI GACGC 1 cut(s) 1279
HhaI GCGC 1 cut(s) 1393
Hin1II CATG 5 cut(s) 71, 122, 287, 461, 689
Hin6I GCGC 1 cut(s) 1391
HinP1I GCGC 1 cut(s) 1391
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 6 cut(s) 32, 72, 578, 713, 760, 897
HpaII CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
HphI GGTGA 2 cut(s) 641, 1306
Hpy166II GTNNAC 2 cut(s) 328, 784
Hpy188I TCNGA 9 cut(s) 37, 571, 759, 778, 981, 1004, 1051, 1111, 1197
Hpy188III TCNNGA 9 cut(s) 113, 214, 230, 246, 398, 445, 734, 901, 1042
Hpy8I GTNNAC 2 cut(s) 328, 784
Hpy99I CGWCG 1 cut(s) 1273
HpyAV CCTTC 5 cut(s) 180, 252, 327, 764, 911
HpyCH4III ACNGT 7 cut(s) 83, 211, 481, 790, 1226, 1304, 1357
HpyCH4IV ACGT 3 cut(s) 491, 865, 1127
HpyCH4V TGCA 4 cut(s) 106, 272, 587, 926
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 1265
HpyF3I CTNAG 5 cut(s) 14, 290, 357, 1040, 1050
HpySE526I ACGT 3 cut(s) 491, 865, 1127
Hsp92II CATG 5 cut(s) 71, 122, 287, 461, 689
HspAI GCGC 1 cut(s) 1391
KroI GCCGGC 1 cut(s) 1393
KroNI GCCGGC 1 cut(s) 1395
Ksp22I TGATCA 2 cut(s) 37, 1111
LguI GCTCTTC 1 cut(s) 1345
LmnI GCTCC 2 cut(s) 157, 1256
Lsp1109I GCAGC 2 cut(s) 46, 1151
LweI GCATC 2 cut(s) 207, 1317
MaeI CTAG 6 cut(s) 29, 113, 183, 339, 914, 1017
MaeII ACGT 3 cut(s) 491, 865, 1127
MaeIII GTNAC 3 cut(s) 77, 481, 1249
MbiI CCGCTC 2 cut(s) 163, 1025
MboII GAAGA 6 cut(s) 258, 261, 460, 558, 1005, 1332
MflI RGATCY 3 cut(s) 374, 563, 600
MhlI GDGCHC 2 cut(s) 1041, 1261
MlsI TGGCCA 1 cut(s) 813
MluCI AATT 3 cut(s) 174, 238, 668
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 3 cut(s) 81, 587, 906
Mox20I TGGCCA 1 cut(s) 813
MroNI GCCGGC 1 cut(s) 1393
MscI TGGCCA 1 cut(s) 813
MseI TTAA 3 cut(s) 411, 873, 1411
Msp20I TGGCCA 1 cut(s) 813
MspI CCGG 7 cut(s) 503, 1178, 1191, 1254, 1292, 1377, 1394
MspR9I CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
Mva1269I GAATGC 1 cut(s) 926
MvaI CCWGG 3 cut(s) 51, 1035, 1085
MwoI GCNNNNNNNGC 2 cut(s) 196, 1265
NaeI GCCGGC 1 cut(s) 1395
NciI CCSGG 2 cut(s) 503, 1377
NcoI CCATGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 549
NgoMIV GCCGGC 1 cut(s) 1393
NlaIII CATG 5 cut(s) 71, 122, 287, 461, 689
NlaIV GGNNCC 4 cut(s) 376, 1258, 1313, 1354
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 481
NspV TTCGAA 1 cut(s) 965
PciSI GCTCTTC 1 cut(s) 1345
PctI GAATGC 1 cut(s) 926
PdiI GCCGGC 1 cut(s) 1395
PfeI GAWTC 3 cut(s) 32, 713, 760
Pfl23II CGTACG 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 311
PfoI TCCNGGA 1 cut(s) 49
PinAI ACCGGT 2 cut(s) 1190, 1291
PkrI GCNGC 3 cut(s) 61, 165, 1166
PleI GAGTC 3 cut(s) 80, 586, 905
PpsI GAGTC 3 cut(s) 80, 586, 905
Ppu21I YACGTR 1 cut(s) 1128
Psp6I CCWGG 3 cut(s) 49, 1033, 1083
PspEI GGTNACC 1 cut(s) 1249
PspFI CCCAGC 2 cut(s) 129, 389
PspGI CCWGG 3 cut(s) 49, 1033, 1083
PspLI CGTACG 1 cut(s) 1151
PspN4I GGNNCC 4 cut(s) 376, 1258, 1313, 1354
PspPI GGNCC 3 cut(s) 179, 314, 1353
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 3 cut(s) 374, 563, 600
RsaI GTAC 3 cut(s) 644, 1153, 1202
RsaNI GTAC 3 cut(s) 643, 1152, 1201
SalI GTCGAC 1 cut(s) 326
SapI GCTCTTC 1 cut(s) 1345
SaqAI TTAA 3 cut(s) 411, 873, 1411
SatI GCNGC 3 cut(s) 60, 164, 1165
Sau96I GGNCC 3 cut(s) 179, 314, 1353
SchI GAGTC 3 cut(s) 81, 587, 906
ScrFI CCNGG 5 cut(s) 51, 503, 1035, 1085, 1377
SduI GDGCHC 2 cut(s) 1041, 1261
SfaNI GCATC 2 cut(s) 207, 1317
SfcI CTRYAG 2 cut(s) 270, 1303
SfuI TTCGAA 1 cut(s) 965
SgrAI CRCCGGYG 1 cut(s) 1291
SinI GGWCC 3 cut(s) 179, 314, 1353
SmlI CTYRAG 1 cut(s) 1274
SmoI CTYRAG 1 cut(s) 1274
SnaBI TACGTA 1 cut(s) 1128
Sse9I AATT 3 cut(s) 174, 238, 668
SsiI CCGC 4 cut(s) 163, 190, 765, 1023
SspMI CTAG 6 cut(s) 29, 113, 183, 339, 914, 1017
StyD4I CCNGG 5 cut(s) 49, 501, 1033, 1083, 1375
StyI CCWWGG 2 cut(s) 67, 317
TaaI ACNGT 7 cut(s) 83, 211, 481, 790, 1226, 1304, 1357
TaiI ACGT 3 cut(s) 494, 868, 1130
TaqI TCGA 3 cut(s) 229, 327, 965
TaqII GACCGA 1 cut(s) 345
TasI AATT 3 cut(s) 174, 238, 668
TatI WGTACW 1 cut(s) 1200
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 3 cut(s) 32, 713, 760
Tru1I TTAA 3 cut(s) 411, 873, 1411
Tru9I TTAA 3 cut(s) 411, 873, 1411
TscAI CASTG 2 cut(s) 490, 1387
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 2 cut(s) 59, 1164
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 9 cut(s) 135, 373, 446, 536, 702, 819, 919, 949, 1112
TspGWI ACGGA 3 cut(s) 611, 651, 1095
TspRI CASTG 2 cut(s) 490, 1387
Van91I CCANNNNNTGG 1 cut(s) 311
VpaK11BI GGWCC 3 cut(s) 179, 314, 1353
XapI RAATTY 1 cut(s) 668
XbaI TCTAGA 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 6 cut(s) 29, 113, 183, 339, 914, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.