Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0004737

Physical Location & Seq

Genomic Coordinates Forward (+)

20521963 .. 20523589

Gene Structure 1627 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g25180.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 657 bp

ATGGTGCTCAAGCTGCATGGACTTTCTGTGTCGACCAACACAGCTCGTGTCGTGGCTTGTCTACATGAGAAAGATGTCGATTTCCAGCTTGTCCCGGTTGATCTCTTTGCCCGTGGAAACAAGGAGCCTTCTTTTCTTGCCAAGAATCCCTTTGGCCAGATTCCAGTATTAGAAGATGGTGACATCACTCTTTTTGAATCTAGGGCAATCTCATCCTACATAGCAGAAAAATTCAAGGAAACCGGAACCGATCTCATACGGCACGAAAGCTTCAGTGAAGCTGCACTGGTGAAGGTTTGGACAGAAGTTGAATCCCAACAATACCACCCAGCAATCTGTCCCATTATATACGAGTTTTTCGGAAAGCCAGTGCAAGGCTTGGAACCAGACCAGGCAGTGATAGATGCAAATTTGGAGAAGCTAGAGATGGTGCTCGATGTTTATGAGGCCAAGCTGAGCAGCACCAAGTACCTTGCTGGTGATTTCTACAGCCTAGCCGATCTTCACCATTTCCCTTACACTTTTTACTTCATGAAGACTCCTTTGGGCTCAGTGATCAGTGATCGTCCTCATGTTAAGGCCTGGTGGGATGACATTACAGCTAGGCCTGCTTTGAAGAAAGTTGCTGAGGGTATGACTTTCGGTGAGAATCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009635 GO:0009636 GO:0009987 GO:0016740 GO:0016765 GO:0019748 GO:0032991 GO:0034641 GO:0042221 GO:0042802 GO:0042803 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044464 GO:0046983 GO:0050896 GO:0051186 GO:0071704 GO:0098754 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_N GST_N_3

Protein Analysis

219

Amino Acids

24.58

Weight (kDa)

5.15

Isoelectric Point (pI)

26.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	1 - 76	4.8e-17	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	5 - 80	3.7e-14	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	11 - 76	2.3e-12	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	109 - 203	1.9e-18	Glutathione S-transferase, C-terminal domain
GST_C_3	PF14497	139 - 207	5.7e-06	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0004737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62760
fragaria_vesca	FvH4_2g25180 FvH4_2g25180 FvH4_5g24660
malus_domestica	MD03G1283000.v1.1 MD06G1012200.v1.1 MD11G1303600.v1.1
prunus_persica	Prupe.5G014900_v2.0.a1 Prupe.8G256600_v2.0.a1 Prupe.8G260300_v2.0.a1
pyrus_communis	pycom06g01020
rosa_chinensis	RchiOBHm_Chr6g0293301 RchiOBHm_Chr7g0215041
rosa_laevigata	RLG00000002697 RLG00000011960
rosa_multiflora	Rmu_ssc0000134.1_g000067 Rmu_ssc0000162.1_g000037
rosa_roxburghii	Rroxscaffold_3G00244640 Rroxscaffold_7G00174270
rosa_rugosa	Rorug06G0234400 Rorug07G0149700
rosa_samantha	Rh6AG347200 Rh6BG354500 Rh6CG361000 Rh6DG347600 Rh7AG286700 Rh7BG278200 Rh7CG306100 Rh7DG290200
rosa_wichuraiana	Rw6G030220 Rw7G024450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	32, 61
AcoI	YGGCCR	1 cut(s)	154
AcsI	RAATTY	2 cut(s)	230, 409
AcuI	CTGAAG	1 cut(s)	256
AfaI	GTAC	1 cut(s)	470
AfiI	CCNNNNNNNGG	1 cut(s)	374
AgsI	TTSAA	4 cut(s)	197, 235, 311, 616
AjnI	CCWGG	2 cut(s)	390, 581
AjuI	GAANNNNNNNTTGG	2 cut(s)	527, 559
AluBI	AGCT	8 cut(s)	13, 44, 88, 270, 281, 421, 454, 602
AluI	AGCT	8 cut(s)	13, 44, 88, 270, 281, 421, 454, 602
Alw21I	GWGCWC	2 cut(s)	9, 435
AoxI	GGCC	4 cut(s)	154, 447, 579, 605
ApeKI	GCWGC	3 cut(s)	13, 281, 459
ApoI	RAATTY	2 cut(s)	230, 409
AsuC2I	CCSGG	1 cut(s)	95
AsuHPI	GGTGA	5 cut(s)	191, 301, 491, 497, 656
BalI	TGGCCA	1 cut(s)	156
BanII	GRGCYC	1 cut(s)	551
BauI	CACGAG	1 cut(s)	45
BbsI	GAAGAC	1 cut(s)	542
Bbv12I	GWGCWC	2 cut(s)	9, 435
BbvCI	CCTCAGC	1 cut(s)	627
BbvI	GCAGC	2 cut(s)	268, 471
BccI	CCATC	2 cut(s)	170, 421
BceAI	ACGGC	1 cut(s)	275
BciT130I	CCWGG	2 cut(s)	392, 583
BclI	TGATCA	1 cut(s)	555
BcnI	CCSGG	1 cut(s)	95
BfaI	CTAG	4 cut(s)	201, 422, 494, 603
BfmI	CTRYAG	1 cut(s)	487
BisI	GCNGC	3 cut(s)	14, 282, 460
BlpI	GCTNAGC	1 cut(s)	455
BlsI	GCNGC	3 cut(s)	15, 283, 461
Bme1390I	CCNGG	3 cut(s)	95, 392, 583
BmiI	GGNNCC	3 cut(s)	126, 247, 384
BmrFI	CCNGG	3 cut(s)	95, 392, 583
BmsI	GCATC	1 cut(s)	394
BpiI	GAAGAC	1 cut(s)	542
Bpu10I	CCTNAGC	1 cut(s)	627
Bpu1102I	GCTNAGC	1 cut(s)	455
BpuMI	CCSGG	1 cut(s)	95
BsaJI	CCNNGG	1 cut(s)	112
BsaWI	WCCGGW	1 cut(s)	242
Bsc4I	CCNNNNNNNGG	1 cut(s)	374
Bse1I	ACTGG	3 cut(s)	164, 291, 368
BseBI	CCWGG	2 cut(s)	392, 583
BseDI	CCNNGG	1 cut(s)	112
BseGI	GGATG	2 cut(s)	212, 595
BseLI	CCNNNNNNNGG	1 cut(s)	374
BseMII	CTCAG	3 cut(s)	446, 564, 618
BseNI	ACTGG	3 cut(s)	164, 291, 368
BseXI	GCAGC	2 cut(s)	268, 471
BseYI	CCCAGC	1 cut(s)	328
BsgI	GTGCAG	1 cut(s)	267
BshFI	GGCC	4 cut(s)	156, 449, 581, 607
BsiHKAI	GWGCWC	2 cut(s)	9, 435
BsiSI	CCGG	2 cut(s)	95, 243
BslFI	GGGAC	2 cut(s)	77, 324
BslI	CCNNNNNNNGG	1 cut(s)	374
BsmFI	GGGAC	2 cut(s)	77, 324
BsnI	GGCC	4 cut(s)	156, 449, 581, 607
Bsp1286I	GDGCHC	3 cut(s)	9, 435, 551
Bsp143I	GATC	5 cut(s)	100, 250, 499, 555, 562
Bsp1720I	GCTNAGC	1 cut(s)	455
BspANI	GGCC	4 cut(s)	156, 449, 581, 607
BspCNI	CTCAG	3 cut(s)	447, 563, 619
BspHI	TCATGA	1 cut(s)	531
BspLI	GGNNCC	3 cut(s)	126, 247, 384
BsrI	ACTGG	3 cut(s)	164, 291, 368
BssECI	CCNNGG	1 cut(s)	112
BssMI	GATC	5 cut(s)	100, 250, 499, 555, 562
BssSI	CACGAG	1 cut(s)	45
Bst2BI	CACGAG	1 cut(s)	45
Bst2UI	CCWGG	2 cut(s)	392, 583
BstC8I	GCNNGC	1 cut(s)	609
BstDEI	CTNAG	3 cut(s)	455, 550, 627
BstDSI	CCRYGG	1 cut(s)	112
BstF5I	GGATG	2 cut(s)	212, 595
BstKTI	GATC	5 cut(s)	103, 253, 502, 558, 565
BstMBI	GATC	5 cut(s)	100, 250, 499, 555, 562
BstMWI	GCNNNNNNNGC	2 cut(s)	13, 608
BstNI	CCWGG	2 cut(s)	392, 583
BstSCI	CCNGG	3 cut(s)	93, 390, 581
BstSFI	CTRYAG	1 cut(s)	487
BstV1I	GCAGC	2 cut(s)	268, 471
BstV2I	GAAGAC	1 cut(s)	542
BsuRI	GGCC	4 cut(s)	156, 449, 581, 607
BtgI	CCRYGG	1 cut(s)	112
BtsCI	GGATG	2 cut(s)	212, 595
BtsI	GCAGTG	1 cut(s)	402
BtsIMutI	CAGTG	6 cut(s)	280, 284, 375, 402, 558, 565
Cac8I	GCNNGC	1 cut(s)	609
CciI	TCATGA	1 cut(s)	531
Csp6I	GTAC	1 cut(s)	469
CviAII	CATG	4 cut(s)	17, 65, 532, 572
CviQI	GTAC	1 cut(s)	469
DdeI	CTNAG	3 cut(s)	455, 550, 627
DpnI	GATC	5 cut(s)	102, 252, 501, 557, 564
DpnII	GATC	5 cut(s)	100, 250, 499, 555, 562
EaeI	YGGCCR	1 cut(s)	154
Eco147I	AGGCCT	2 cut(s)	581, 607
Eco24I	GRGCYC	1 cut(s)	551
Eco57I	CTGAAG	1 cut(s)	256
EcoRII	CCWGG	2 cut(s)	390, 581
EcoT38I	GRGCYC	1 cut(s)	551
FaeI	CATG	4 cut(s)	20, 68, 535, 575
FalI	AAGNNNNNCTT	2 cut(s)	134, 166
FaqI	GGGAC	2 cut(s)	77, 324
FatI	CATG	4 cut(s)	16, 64, 531, 571
FbaI	TGATCA	1 cut(s)	555
FblI	GTMKAC	2 cut(s)	32, 61
Fnu4HI	GCNGC	3 cut(s)	14, 282, 460
FokI	GGATG	2 cut(s)	199, 602
FriOI	GRGCYC	1 cut(s)	551
Fsp4HI	GCNGC	3 cut(s)	14, 282, 460
FspBI	CTAG	4 cut(s)	201, 422, 494, 603
GluI	GCNGC	3 cut(s)	14, 282, 460
GsaI	CCCAGC	1 cut(s)	332
HaeIII	GGCC	4 cut(s)	156, 449, 581, 607
HapII	CCGG	2 cut(s)	95, 243
Hin1II	CATG	4 cut(s)	20, 68, 535, 575
HincII	GTYRAC	1 cut(s)	33
HindII	GTYRAC	1 cut(s)	33
HindIII	AAGCTT	1 cut(s)	268
HinfI	GANTC	6 cut(s)	145, 160, 197, 311, 538, 649
HpaII	CCGG	2 cut(s)	95, 243
HphI	GGTGA	5 cut(s)	191, 301, 491, 497, 656
Hpy166II	GTNNAC	2 cut(s)	33, 62
Hpy188I	TCNGA	1 cut(s)	362
Hpy188III	TCNNGA	1 cut(s)	532
Hpy8I	GTNNAC	2 cut(s)	33, 62
HpyAV	CCTTC	2 cut(s)	138, 286
HpyCH4V	TGCA	4 cut(s)	16, 284, 373, 407
HpyF10VI	GCNNNNNNNGC	2 cut(s)	13, 608
HpyF3I	CTNAG	3 cut(s)	455, 550, 627
Hsp92II	CATG	4 cut(s)	20, 68, 535, 575
Ksp22I	TGATCA	1 cut(s)	555
Kzo9I	GATC	5 cut(s)	100, 250, 499, 555, 562
LmnI	GCTCC	1 cut(s)	124
Lsp1109I	GCAGC	2 cut(s)	268, 471
LweI	GCATC	1 cut(s)	394
MaeI	CTAG	4 cut(s)	201, 422, 494, 603
MaeIII	GTNAC	1 cut(s)	179
MalI	GATC	5 cut(s)	102, 252, 501, 557, 564
MboI	GATC	5 cut(s)	100, 250, 499, 555, 562
MboII	GAAGA	4 cut(s)	185, 494, 547, 628
MhlI	GDGCHC	3 cut(s)	9, 435, 551
MlsI	TGGCCA	1 cut(s)	156
MluCI	AATT	2 cut(s)	230, 409
MluNI	TGGCCA	1 cut(s)	156
MlyI	GAGTC	1 cut(s)	532
MnlI	CCTC	3 cut(s)	439, 579, 622
Mox20I	TGGCCA	1 cut(s)	156
MscI	TGGCCA	1 cut(s)	156
MseI	TTAA	1 cut(s)	576
Msp20I	TGGCCA	1 cut(s)	156
MspI	CCGG	2 cut(s)	95, 243
MspR9I	CCNGG	3 cut(s)	95, 392, 583
MvaI	CCWGG	2 cut(s)	392, 583
MwoI	GCNNNNNNNGC	2 cut(s)	13, 608
NciI	CCSGG	1 cut(s)	95
NdeII	GATC	5 cut(s)	100, 250, 499, 555, 562
NlaIII	CATG	4 cut(s)	20, 68, 535, 575
NlaIV	GGNNCC	3 cut(s)	126, 247, 384
NmuCI	GTSAC	1 cut(s)	179
PagI	TCATGA	1 cut(s)	531
PceI	AGGCCT	2 cut(s)	581, 607
PfeI	GAWTC	5 cut(s)	145, 160, 197, 311, 649
PkrI	GCNGC	3 cut(s)	15, 283, 461
PleI	GAGTC	1 cut(s)	532
PpsI	GAGTC	1 cut(s)	532
Psp6I	CCWGG	2 cut(s)	390, 581
PspFI	CCCAGC	1 cut(s)	328
PspGI	CCWGG	2 cut(s)	390, 581
PspN4I	GGNNCC	3 cut(s)	126, 247, 384
RsaI	GTAC	1 cut(s)	470
RsaNI	GTAC	1 cut(s)	469
SalI	GTCGAC	1 cut(s)	31
SaqAI	TTAA	1 cut(s)	576
SatI	GCNGC	3 cut(s)	14, 282, 460
Sau3AI	GATC	5 cut(s)	100, 250, 499, 555, 562
SchI	GAGTC	1 cut(s)	532
ScrFI	CCNGG	3 cut(s)	95, 392, 583
SduI	GDGCHC	3 cut(s)	9, 435, 551
SfaNI	GCATC	1 cut(s)	394
SfcI	CTRYAG	1 cut(s)	487
SmlI	CTYRAG	1 cut(s)	8
SmoI	CTYRAG	1 cut(s)	8
Sse9I	AATT	2 cut(s)	230, 409
SseBI	AGGCCT	2 cut(s)	581, 607
SspMI	CTAG	4 cut(s)	201, 422, 494, 603
StuI	AGGCCT	2 cut(s)	581, 607
StyD4I	CCNGG	3 cut(s)	93, 390, 581
TaqI	TCGA	3 cut(s)	32, 78, 435
TasI	AATT	2 cut(s)	230, 409
TfiI	GAWTC	5 cut(s)	145, 160, 197, 311, 649
Tru1I	TTAA	1 cut(s)	576
Tru9I	TTAA	1 cut(s)	576
TscAI	CASTG	6 cut(s)	280, 291, 375, 402, 558, 565
TseFI	GTSAC	1 cut(s)	179
TseI	GCWGC	3 cut(s)	13, 281, 459
Tsp45I	GTSAC	1 cut(s)	179
TspDTI	ATGAA	2 cut(s)	520, 548
TspRI	CASTG	6 cut(s)	280, 291, 375, 402, 558, 565
XapI	RAATTY	2 cut(s)	230, 409
XmiI	GTMKAC	2 cut(s)	32, 61
XspI	CTAG	4 cut(s)	201, 422, 494, 603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.