MD06G1012200.v1.1
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004737
Physical Location & Seq
Forward (+)
1531953 .. 1534667
2715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1012200.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGGAGTGAAAGTGCATGGTGTTGCTGTCTCGACATGCACATCTCGTGTCTTAACCATTCTCCATGAAAAAGGCCTTGATTTTGAGCTCGTCCCTGTTAACCTTCTTGCAGGAGAACACAAGCAGCCCCCTTTCCTCGCCAAAAATCCTTTCGGACAGATTCCAGTGCTTGAAGATGGTGATCTCACTCTTTTTGAGTCAAGAGCTATCACTGCATATGTGGCTGAGAAATTCAAGGAAAATGGTGTTGATCTGATCAGGCACAACAACCTCAATGAGGCAGCTCTGGTCAAGGTCTGGACAGAGGTGGAGTCCCAGAGTTTCCACCCTGCAATCTCTCCCATTGTCTTCCAACATATCATCGTCCCCATGCGCGGACAAACACCGGATCAGGCAGTCATCGACGCCAACTTGGAGAAGCTCGCGAAAGTTCTGGACGTGTACGAGGACAGGCTGAGTAGCACCAAGTACCTGGCTGGTGATTTCTACAGCCTTGCTGATCTCCACCACCTTTCCTACACTTACTACTTCATGAAGACTCCGGGGGCGTCGTTGGTGAATGAACGTCCACACGTTAAGGCTTGGTGGGAGGACATTTCTTCTAGGCCTGCTTTCAAGAAAGTGGCCGAGGCTATGACCTTGGGACAAAAGTAG

Protein Analysis

218

Amino Acids

24.28

Weight (kDa)

6.13

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_3 PF13417 6 - 81 4.7e-14 Glutathione S-transferase, N-terminal domain
GST_N PF02798 11 - 76 3.8e-18 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 11 - 76 2.4e-14 Glutathione S-transferase, N-terminal domain
GST_C PF00043 116 - 203 3.6e-18 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 138 - 207 3e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 375, 425
AciI CCGC 1 cut(s) 375
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 1 cut(s) 230
AcyI GRCGYC 2 cut(s) 405, 548
AfaI GTAC 2 cut(s) 443, 470
AfiI CCNNNNNNNGG 2 cut(s) 277, 374
AflIII ACRYGT 2 cut(s) 438, 571
AgsI TTSAA 3 cut(s) 173, 235, 616
AjiI CACGTC 1 cut(s) 439
AjnI CCWGG 1 cut(s) 471
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AluBI AGCT 4 cut(s) 88, 206, 284, 421
AluI AGCT 4 cut(s) 88, 206, 284, 421
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 34
AlwI GGATC 1 cut(s) 396
AoxI GGCC 3 cut(s) 73, 605, 624
ApeKI GCWGC 2 cut(s) 124, 281
ApoI RAATTY 1 cut(s) 230
AspLEI GCGC 1 cut(s) 375
AsuC2I CCSGG 1 cut(s) 543
AsuHPI GGTGA 3 cut(s) 191, 491, 568
BanII GRGCYC 1 cut(s) 90
BauI CACGAG 1 cut(s) 45
BbsI GAAGAC 2 cut(s) 340, 542
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 136, 293
BccI CCATC 1 cut(s) 170
BciT130I CCWGG 1 cut(s) 473
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 543
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 1 cut(s) 603
BfmI CTRYAG 1 cut(s) 487
BisI GCNGC 2 cut(s) 125, 282
BlsI GCNGC 2 cut(s) 126, 283
Bme1390I CCNGG 2 cut(s) 473, 543
BmgBI CACGTC 1 cut(s) 439
BmrFI CCNGG 2 cut(s) 473, 543
BpiI GAAGAC 2 cut(s) 340, 542
BpuMI CCSGG 1 cut(s) 543
BsaBI GATNNNNATC 1 cut(s) 180
BsaHI GRCGYC 2 cut(s) 405, 548
BsaJI CCNNGG 3 cut(s) 542, 627, 639
BsaWI WCCGGW 1 cut(s) 385
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 374
Bse1I ACTGG 1 cut(s) 164
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 3 cut(s) 542, 627, 639
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 277, 374
BseMII CTCAG 2 cut(s) 216, 446
BseNI ACTGG 1 cut(s) 164
BseXI GCAGC 2 cut(s) 136, 293
Bsh1236I CGCG 2 cut(s) 375, 425
BshFI GGCC 3 cut(s) 75, 607, 626
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 2 cut(s) 386, 542
BslFI GGGAC 3 cut(s) 77, 298, 350
BslI CCNNNNNNNGG 2 cut(s) 277, 374
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 3 cut(s) 77, 298, 350
BsnI GGCC 3 cut(s) 75, 607, 626
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 5 cut(s) 181, 250, 255, 388, 499
Bsp68I TCGCGA 1 cut(s) 425
BspACI CCGC 1 cut(s) 375
BspANI GGCC 3 cut(s) 75, 607, 626
BspCNI CTCAG 2 cut(s) 217, 447
BspFNI CGCG 2 cut(s) 375, 425
BspHI TCATGA 1 cut(s) 531
BspPI GGATC 1 cut(s) 396
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 3 cut(s) 542, 627, 639
BssMI GATC 5 cut(s) 181, 250, 255, 388, 499
BssNI GRCGYC 2 cut(s) 405, 548
BssSI CACGAG 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 639
Bst2BI CACGAG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 473
BstACI GRCGYC 2 cut(s) 405, 548
BstC8I GCNNGC 2 cut(s) 423, 609
BstDEI CTNAG 2 cut(s) 225, 455
BstENI CCTNNNNNAGG 1 cut(s) 275
BstFNI CGCG 2 cut(s) 375, 425
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 5 cut(s) 184, 253, 258, 391, 502
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 5 cut(s) 181, 250, 255, 388, 499
BstMWI GCNNNNNNNGC 2 cut(s) 212, 221
BstNI CCWGG 1 cut(s) 473
BstNSI RCATGY 1 cut(s) 39
BstSCI CCNGG 2 cut(s) 471, 541
BstSFI CTRYAG 1 cut(s) 487
BstUI CGCG 2 cut(s) 375, 425
BstV1I GCAGC 2 cut(s) 136, 293
BstV2I GAAGAC 2 cut(s) 340, 542
BstXI CCANNNNNNTGG 1 cut(s) 472
BsuRI GGCC 3 cut(s) 75, 607, 626
BtrI CACGTC 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 171, 210
BtuMI TCGCGA 1 cut(s) 425
Cac8I GCNNGC 2 cut(s) 423, 609
CciI TCATGA 1 cut(s) 531
CfoI GCGC 1 cut(s) 375
CseI GACGC 2 cut(s) 413, 537
Csp6I GTAC 2 cut(s) 442, 469
CviAII CATG 5 cut(s) 17, 36, 65, 370, 532
CviQI GTAC 2 cut(s) 442, 469
DdeI CTNAG 2 cut(s) 225, 455
DpnI GATC 5 cut(s) 183, 252, 257, 390, 501
DpnII GATC 5 cut(s) 181, 250, 255, 388, 499
EaeI YGGCCR 1 cut(s) 624
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 639
Eco147I AGGCCT 2 cut(s) 75, 607
Eco24I GRGCYC 1 cut(s) 90
Eco53kI GAGCTC 1 cut(s) 88
EcoICRI GAGCTC 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 639
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 639
FaeI CATG 5 cut(s) 20, 39, 68, 373, 535
FaiI YATR 9 cut(s) 18, 37, 66, 217, 219, 357, 371, 533, 635
FaqI GGGAC 3 cut(s) 77, 298, 350
FatI CATG 5 cut(s) 16, 35, 64, 369, 531
FauNDI CATATG 1 cut(s) 217
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 2 cut(s) 125, 282
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 2 cut(s) 125, 282
FspBI CTAG 1 cut(s) 603
GlaI GCGC 1 cut(s) 374
GluI GCNGC 2 cut(s) 125, 282
HaeIII GGCC 3 cut(s) 75, 607, 626
HapII CCGG 2 cut(s) 386, 542
HgaI GACGC 2 cut(s) 413, 537
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 2 cut(s) 405, 548
Hin1II CATG 5 cut(s) 20, 39, 68, 373, 535
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 4 cut(s) 160, 197, 311, 538
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 2 cut(s) 386, 542
HphI GGTGA 3 cut(s) 191, 491, 568
Hpy166II GTNNAC 3 cut(s) 100, 442, 569
Hpy188I TCNGA 2 cut(s) 155, 255
Hpy188III TCNNGA 7 cut(s) 31, 201, 298, 424, 434, 532, 616
Hpy8I GTNNAC 3 cut(s) 100, 442, 569
Hpy99I CGWCG 2 cut(s) 407, 553
HpyAV CCTTC 1 cut(s) 113
HpyCH4IV ACGT 3 cut(s) 438, 565, 573
HpyCH4V TGCA 5 cut(s) 16, 39, 110, 215, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 221
HpyF3I CTNAG 2 cut(s) 225, 455
HpySE526I ACGT 3 cut(s) 438, 565, 573
Hsp92I GRCGYC 2 cut(s) 405, 548
Hsp92II CATG 5 cut(s) 20, 39, 68, 373, 535
HspAI GCGC 1 cut(s) 373
Ksp22I TGATCA 1 cut(s) 255
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 5 cut(s) 181, 250, 255, 388, 499
Lsp1109I GCAGC 2 cut(s) 136, 293
MaeI CTAG 1 cut(s) 603
MaeII ACGT 3 cut(s) 438, 565, 573
MalI GATC 5 cut(s) 183, 252, 257, 390, 501
MboI GATC 5 cut(s) 181, 250, 255, 388, 499
MboII GAAGA 4 cut(s) 185, 340, 547, 591
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 1 cut(s) 230
MlyI GAGTC 3 cut(s) 206, 320, 532
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 7 cut(s) 146, 271, 281, 298, 439, 583, 622
MseI TTAA 3 cut(s) 53, 99, 576
MspI CCGG 2 cut(s) 386, 542
MspR9I CCNGG 2 cut(s) 473, 543
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 2 cut(s) 375, 425
MwoI GCNNNNNNNGC 2 cut(s) 212, 221
NciI CCSGG 1 cut(s) 543
NdeI CATATG 1 cut(s) 217
NdeII GATC 5 cut(s) 181, 250, 255, 388, 499
NlaIII CATG 5 cut(s) 20, 39, 68, 373, 535
NmeAIII GCCGAG 1 cut(s) 652
NruI TCGCGA 1 cut(s) 425
NspI RCATGY 1 cut(s) 39
PagI TCATGA 1 cut(s) 531
PceI AGGCCT 2 cut(s) 75, 607
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 2 cut(s) 126, 283
PleI GAGTC 3 cut(s) 205, 319, 532
PpsI GAGTC 3 cut(s) 205, 319, 532
Psp124BI GAGCTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
RruI TCGCGA 1 cut(s) 425
RsaI GTAC 2 cut(s) 443, 470
RsaNI GTAC 2 cut(s) 442, 469
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 3 cut(s) 53, 99, 576
SatI GCNGC 2 cut(s) 125, 282
Sau3AI GATC 5 cut(s) 181, 250, 255, 388, 499
SchI GAGTC 3 cut(s) 206, 320, 532
ScrFI CCNGG 2 cut(s) 473, 543
SduI GDGCHC 1 cut(s) 90
SfcI CTRYAG 1 cut(s) 487
Sse9I AATT 1 cut(s) 230
SseBI AGGCCT 2 cut(s) 75, 607
SsiI CCGC 1 cut(s) 375
SspMI CTAG 1 cut(s) 603
SstI GAGCTC 1 cut(s) 90
StuI AGGCCT 2 cut(s) 75, 607
StyD4I CCNGG 2 cut(s) 471, 541
StyI CCWWGG 1 cut(s) 639
TaiI ACGT 3 cut(s) 441, 568, 576
TaqI TCGA 2 cut(s) 32, 402
TasI AATT 1 cut(s) 230
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 3 cut(s) 53, 99, 576
Tru9I TTAA 3 cut(s) 53, 99, 576
TscAI CASTG 2 cut(s) 171, 217
TseI GCWGC 2 cut(s) 124, 281
TspDTI ATGAA 4 cut(s) 81, 520, 548, 576
TspRI CASTG 2 cut(s) 171, 217
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 1 cut(s) 230
XceI RCATGY 1 cut(s) 39
XspI CTAG 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.