Rw7G024450
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004737
Physical Location & Seq
Reverse (-)
30081502 .. 30082753
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G024450.1

Sequence Viewer

Length: 654 bp
ATGGCACTCAAGCTTCATGGCCTGGCTTTCTCAACATGCACAGCTCGTGCCGCAACCGCCATTTACGAGAAAGGACAGCAATTCGAGCTTGTCCCTGTCAACCTTACTGCAGGAGAACACAAGCAGCCAGGTTTCCTCGCCAAAAATCCCTTTGGCCAAATTCCAGCCCTAGAGGATGGTGATCTCACTCTTTTTGAGTCTAGAGCAATTGCAACATATGTGGCTGAAAAATACAAGGACTCGGGTTGCGATCTGATCAGGCACCAAAACCTCAAGGAAGCTGCACTTGTCAAGGTGTGGTCGGAGGTGGAGTCCCAGACCTTCCACCCCGTAATCTCCGCCATTGTCTACCAGTTTCTCATTGCGCCATTGTTCGGACAGCAGACAGACCAAGCCGTGATCGACGCAAACCTGGAGAAGCTCAAGAACATCCTCGATGTCTACGAGTCAAGGTTGAACAGCAGCAAGTACTTGGCAGGAGATTTCTTCAGCCTTGCAGATCTTAACCACTGTCCCTACCTTTTCTACTTCATGAGGAGTCCGTGGACTTCGGTGGTGAATGAACGTCCTCATGTGAAAGCCTGGTGGGAGGACATTTCTTCAAGGCCAAGCTTCAAGAAAGTGGCCGAGGGAATGACCTTTGGTCAGAAGTAG

Protein Analysis

217

Amino Acids

24.44

Weight (kDa)

6.3

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 76 1.8e-19 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 5 - 80 7.9e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 10 - 76 1.9e-12 Glutathione S-transferase, N-terminal domain
GST_C PF00043 112 - 203 2.4e-17 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 136 - 207 8.2e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 261
AccI GTMKAC 2 cut(s) 348, 441
AciI CCGC 3 cut(s) 51, 57, 339
AcoI YGGCCR 2 cut(s) 154, 624
AcsI RAATTY 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 472
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 1 cut(s) 374
AgsI TTSAA 3 cut(s) 457, 603, 616
AhdI GACNNNNNGTC 1 cut(s) 642
AjnI CCWGG 4 cut(s) 21, 127, 411, 581
AluBI AGCT 6 cut(s) 13, 44, 88, 281, 421, 612
AluI AGCT 6 cut(s) 13, 44, 88, 281, 421, 612
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 4 cut(s) 19, 154, 605, 624
ApeKI GCWGC 3 cut(s) 124, 281, 462
ApoI RAATTY 1 cut(s) 159
AspLEI GCGC 1 cut(s) 367
AsuHPI GGTGA 2 cut(s) 191, 568
AvaI CYCGRG 1 cut(s) 241
BalI TGGCCA 1 cut(s) 156
BanI GGYRCC 1 cut(s) 261
BauI CACGAG 1 cut(s) 45
BbvI GCAGC 3 cut(s) 136, 268, 474
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 380
BciT130I CCWGG 4 cut(s) 23, 129, 413, 583
BclI TGATCA 1 cut(s) 255
BfaI CTAG 2 cut(s) 170, 201
BfmI CTRYAG 1 cut(s) 108
BglII AGATCT 1 cut(s) 499
BisI GCNGC 4 cut(s) 51, 125, 282, 463
BlsI GCNGC 4 cut(s) 52, 126, 283, 464
BmcAI AGTACT 1 cut(s) 470
Bme1390I CCNGG 4 cut(s) 23, 129, 413, 583
BmeRI GACNNNNNGTC 1 cut(s) 642
BmeT110I CYCGRG 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 4 cut(s) 23, 129, 413, 583
BpmI CTGGAG 1 cut(s) 434
BpuEI CTTGAG 2 cut(s) 257, 407
BsaBI GATNNNNATC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 542, 627
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 180
BseBI CCWGG 4 cut(s) 23, 129, 413, 583
BseDI CCNNGG 2 cut(s) 542, 627
BseGI GGATG 2 cut(s) 181, 429
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMI GCAATG 1 cut(s) 360
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 550
BseXI GCAGC 3 cut(s) 136, 268, 474
BsgI GTGCAG 1 cut(s) 267
BshFI GGCC 4 cut(s) 21, 156, 607, 626
BshNI GGYRCC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 241
BslFI GGGAC 3 cut(s) 77, 298, 498
BslI CCNNNNNNNGG 1 cut(s) 374
BsmFI GGGAC 3 cut(s) 77, 298, 498
BsnI GGCC 4 cut(s) 21, 156, 607, 626
BsoBI CYCGRG 1 cut(s) 241
Bsp143I GATC 5 cut(s) 181, 250, 255, 399, 499
BspACI CCGC 3 cut(s) 51, 57, 339
BspANI GGCC 4 cut(s) 21, 156, 607, 626
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 1 cut(s) 263
BspMAI CTGCAG 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 261
BsrDI GCAATG 1 cut(s) 360
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 542, 627
BssMI GATC 5 cut(s) 181, 250, 255, 399, 499
BssSI CACGAG 1 cut(s) 45
Bst2BI CACGAG 1 cut(s) 45
Bst2UI CCWGG 4 cut(s) 23, 129, 413, 583
Bst4CI ACNGT 1 cut(s) 512
BstDSI CCRYGG 1 cut(s) 542
BstF5I GGATG 2 cut(s) 181, 429
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 5 cut(s) 184, 253, 258, 402, 502
BstMBI GATC 5 cut(s) 181, 250, 255, 399, 499
BstMWI GCNNNNNNNGC 3 cut(s) 50, 56, 85
BstNI CCWGG 4 cut(s) 23, 129, 413, 583
BstNSI RCATGY 1 cut(s) 39
BstSCI CCNGG 4 cut(s) 21, 127, 411, 581
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 3 cut(s) 136, 268, 474
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 4 cut(s) 21, 156, 607, 626
BtgI CCRYGG 1 cut(s) 542
BtsCI GGATG 2 cut(s) 181, 429
BtsIMutI CAGTG 1 cut(s) 508
CciI TCATGA 1 cut(s) 531
CfoI GCGC 1 cut(s) 367
CseI GACGC 1 cut(s) 413
Csp6I GTAC 1 cut(s) 469
CspCI CAANNNNNGTGG 2 cut(s) 201, 236
CviAII CATG 4 cut(s) 17, 36, 532, 572
CviQI GTAC 1 cut(s) 469
DpnI GATC 5 cut(s) 183, 252, 257, 401, 501
DpnII GATC 5 cut(s) 181, 250, 255, 399, 499
DriI GACNNNNNGTC 1 cut(s) 642
EaeI YGGCCR 2 cut(s) 154, 624
Eam1105I GACNNNNNGTC 1 cut(s) 642
EciI GGCGGA 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 472
Eco88I CYCGRG 1 cut(s) 241
EcoRII CCWGG 4 cut(s) 21, 127, 411, 581
FaeI CATG 4 cut(s) 20, 39, 535, 575
FaiI YATR 6 cut(s) 18, 37, 217, 219, 533, 573
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 3 cut(s) 77, 298, 498
FatI CATG 4 cut(s) 16, 35, 531, 571
FauNDI CATATG 1 cut(s) 217
FbaI TGATCA 1 cut(s) 255
FblI GTMKAC 2 cut(s) 348, 441
Fnu4HI GCNGC 4 cut(s) 51, 125, 282, 463
FokI GGATG 2 cut(s) 188, 416
Fsp4HI GCNGC 4 cut(s) 51, 125, 282, 463
FspBI CTAG 2 cut(s) 170, 201
GlaI GCGC 1 cut(s) 366
GluI GCNGC 4 cut(s) 51, 125, 282, 463
GsuI CTGGAG 1 cut(s) 434
HaeIII GGCC 4 cut(s) 21, 156, 607, 626
HgaI GACGC 1 cut(s) 413
HhaI GCGC 1 cut(s) 367
Hin1II CATG 4 cut(s) 20, 39, 535, 575
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 2 cut(s) 11, 610
HinfI GANTC 5 cut(s) 197, 239, 311, 446, 538
HphI GGTGA 2 cut(s) 191, 568
Hpy166II GTNNAC 4 cut(s) 100, 349, 442, 546
Hpy188I TCNGA 4 cut(s) 255, 304, 377, 648
Hpy188III TCNNGA 4 cut(s) 201, 424, 532, 616
Hpy8I GTNNAC 4 cut(s) 100, 349, 442, 546
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 1 cut(s) 512
HpyCH4IV ACGT 1 cut(s) 565
HpyCH4V TGCA 5 cut(s) 39, 110, 212, 284, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 56, 85
HpySE526I ACGT 1 cut(s) 565
Hsp92II CATG 4 cut(s) 20, 39, 535, 575
HspAI GCGC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 5 cut(s) 181, 250, 255, 399, 499
Lsp1109I GCAGC 3 cut(s) 136, 268, 474
MaeI CTAG 2 cut(s) 170, 201
MaeII ACGT 1 cut(s) 565
MalI GATC 5 cut(s) 183, 252, 257, 401, 501
MboI GATC 5 cut(s) 181, 250, 255, 399, 499
MboII GAAGA 2 cut(s) 478, 591
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 1 cut(s) 499
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 3 cut(s) 80, 159, 207
MluNI TGGCCA 1 cut(s) 156
MlyI GAGTC 5 cut(s) 206, 233, 320, 455, 547
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 9 cut(s) 146, 166, 281, 298, 443, 528, 579, 583, 622
Mox20I TGGCCA 1 cut(s) 156
MscI TGGCCA 1 cut(s) 156
MseI TTAA 1 cut(s) 504
Msp20I TGGCCA 1 cut(s) 156
MspR9I CCNGG 4 cut(s) 23, 129, 413, 583
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 4 cut(s) 23, 129, 413, 583
MwoI GCNNNNNNNGC 3 cut(s) 50, 56, 85
NdeI CATATG 1 cut(s) 217
NdeII GATC 5 cut(s) 181, 250, 255, 399, 499
NlaIII CATG 4 cut(s) 20, 39, 535, 575
NlaIV GGNNCC 1 cut(s) 263
NmeAIII GCCGAG 1 cut(s) 652
NspI RCATGY 1 cut(s) 39
PagI TCATGA 1 cut(s) 531
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PkrI GCNGC 4 cut(s) 52, 126, 283, 464
PleI GAGTC 5 cut(s) 205, 233, 319, 454, 546
PpsI GAGTC 5 cut(s) 205, 233, 319, 454, 546
Psp6I CCWGG 4 cut(s) 21, 127, 411, 581
PspGI CCWGG 4 cut(s) 21, 127, 411, 581
PspN4I GGNNCC 1 cut(s) 263
PstI CTGCAG 1 cut(s) 112
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 4 cut(s) 51, 125, 282, 463
Sau3AI GATC 5 cut(s) 181, 250, 255, 399, 499
ScaI AGTACT 1 cut(s) 470
SchI GAGTC 5 cut(s) 206, 233, 320, 455, 547
ScrFI CCNGG 4 cut(s) 23, 129, 413, 583
SfcI CTRYAG 1 cut(s) 108
SmlI CTYRAG 3 cut(s) 8, 272, 422
SmoI CTYRAG 3 cut(s) 8, 272, 422
Sse9I AATT 3 cut(s) 80, 159, 207
SsiI CCGC 3 cut(s) 51, 57, 339
SspMI CTAG 2 cut(s) 170, 201
StyD4I CCNGG 4 cut(s) 21, 127, 411, 581
TaaI ACNGT 1 cut(s) 512
TaiI ACGT 1 cut(s) 568
TaqI TCGA 3 cut(s) 84, 402, 435
TasI AATT 3 cut(s) 80, 159, 207
TatI WGTACW 1 cut(s) 468
TauI GCSGC 1 cut(s) 53
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 1 cut(s) 515
TseI GCWGC 3 cut(s) 124, 281, 462
TspDTI ATGAA 3 cut(s) 5, 520, 576
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 1 cut(s) 515
XapI RAATTY 1 cut(s) 159
XbaI TCTAGA 1 cut(s) 200
XceI RCATGY 1 cut(s) 39
XmiI GTMKAC 2 cut(s) 348, 441
XspI CTAG 2 cut(s) 170, 201
ZrmI AGTACT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.