Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004737

Physical Location & Seq

Genomic Coordinates Reverse (-)

36329385 .. 36330432

Gene Structure 1048 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1283000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGGTGCTCAAGCTGCATGGACTTTCTGTGTCTATTTCCACAGCTCGCGTCGTCGCTTGTCTCCATGAAAAAAGCGTTGATTTCGAGCTTGTCCCCGTTGATCTCTTCGCCTGCGAAAACAAGCAGCCTGAATTTCTAACCAAGAATCCCTTTGGCAAAGTTCCAGTACTAGAAGACGATGACATCACACTTTTCGAATCTAGGGCAATCACAGCATATGTAGCTGAAAAATTCAAGGAAACGGGACATGATCTCATTCGTCACGAAAACTTCAACGAGGCTGCATTGGTGAAGGTATGGACAGAAGTGGAATCTCAACAATACAACCCTGCAATCGATCCAATTATTTTCGAGTTTTTCGCAAAGCCTGTTGTAGGAATGGAACCAGATCAAACAGTGATGGATGCAAGTTTGGAGAAGCTGAAGAAGGTGCTGGACGTGTACGAAACCAGGCTGAGCAACAACAAGTTCCTTGCTGGTGATTTCTACAGCCTAGCTGACCTTCACCACTTTCCCGGCACTTTTTATTTCATGAAGACGCCTTGGTCTTCGTTGGTCCACGATCGTCCTCACGTCAAGGCGTGGTGGGAGGAGATTTCATCTAGGCCTGCCTCCAAGAAAGTGGCTGAGGGTATGAATTTTGGTGAAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006518 GO:0006575 GO:0006749 GO:0006790 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009404 GO:0009407 GO:0009635 GO:0009636 GO:0009987 GO:0016740 GO:0016765 GO:0019748 GO:0032991 GO:0034641 GO:0042221 GO:0042802 GO:0042803 GO:0043603 GO:0044237 GO:0044248 GO:0044424 GO:0044464 GO:0046983 GO:0050896 GO:0051186 GO:0071704 GO:0098754 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418

Pfam Domains

Protein Families

GST_C GST_N GST_N_3

Protein Analysis

218

Amino Acids

24.74

Weight (kDa)

5.28

Isoelectric Point (pI)

32.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GST_N	PF02798	1 - 76	5.3e-18	Glutathione S-transferase, N-terminal domain
GST_N_3	PF13417	5 - 80	7.9e-13	Glutathione S-transferase, N-terminal domain
GST_N_2	PF13409	14 - 76	1.3e-12	Glutathione S-transferase, N-terminal domain
GST_C	PF00043	118 - 203	1.4e-18	Glutathione S-transferase, C-terminal domain
GST_C_3	PF14497	136 - 204	1.2e-06	Glutathione S-transferase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G62760
fragaria_vesca	FvH4_2g25180 FvH4_2g25180 FvH4_5g24660
malus_domestica	MD03G1283000.v1.1 MD06G1012200.v1.1 MD11G1303600.v1.1
prunus_persica	Prupe.5G014900_v2.0.a1 Prupe.8G256600_v2.0.a1 Prupe.8G260300_v2.0.a1
pyrus_communis	pycom06g01020
rosa_chinensis	RchiOBHm_Chr6g0293301 RchiOBHm_Chr7g0215041
rosa_laevigata	RLG00000002697 RLG00000011960
rosa_multiflora	Rmu_ssc0000134.1_g000067 Rmu_ssc0000162.1_g000037
rosa_roxburghii	Rroxscaffold_3G00244640 Rroxscaffold_7G00174270
rosa_rugosa	Rorug06G0234400 Rorug07G0149700
rosa_samantha	Rh6AG347200 Rh6BG354500 Rh6CG361000 Rh6DG347600 Rh7AG286700 Rh7BG278200 Rh7CG306100 Rh7DG290200
rosa_wichuraiana	Rw6G030220 Rw7G024450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	544
AccII	CGCG	1 cut(s)	48
AclWI	GGATC	1 cut(s)	332
AcsI	RAATTY	3 cut(s)	131, 230, 637
AcuI	CTGAAG	1 cut(s)	443
AcyI	GRCGYC	1 cut(s)	539
AfaI	GTAC	2 cut(s)	168, 443
AfiI	CCNNNNNNNGG	1 cut(s)	374
AflIII	ACRYGT	1 cut(s)	438
AgsI	TTSAA	2 cut(s)	235, 274
AjiI	CACGTC	2 cut(s)	439, 574
AjnI	CCWGG	1 cut(s)	449
AluBI	AGCT	6 cut(s)	13, 44, 88, 224, 421, 497
AluI	AGCT	6 cut(s)	13, 44, 88, 224, 421, 497
Alw21I	GWGCWC	1 cut(s)	9
Alw26I	GTCTC	1 cut(s)	65
AlwI	GGATC	1 cut(s)	332
AoxI	GGCC	1 cut(s)	605
ApeKI	GCWGC	3 cut(s)	13, 124, 281
ApoI	RAATTY	3 cut(s)	131, 230, 637
AspS9I	GGNCC	1 cut(s)	556
AsuC2I	CCSGG	1 cut(s)	516
AsuHPI	GGTGA	3 cut(s)	301, 491, 497
AsuII	TTCGAA	1 cut(s)	195
AvaII	GGWCC	1 cut(s)	556
BbsI	GAAGAC	3 cut(s)	180, 540, 542
Bbv12I	GWGCWC	1 cut(s)	9
BbvCI	CCTCAGC	1 cut(s)	627
BbvI	GCAGC	2 cut(s)	136, 268
BccI	CCATC	1 cut(s)	394
BciT130I	CCWGG	1 cut(s)	451
BcnI	CCSGG	1 cut(s)	516
BcoDI	GTCTC	1 cut(s)	65
BfaI	CTAG	4 cut(s)	170, 201, 494, 603
BfmI	CTRYAG	1 cut(s)	487
BisI	GCNGC	3 cut(s)	14, 125, 282
BlpI	GCTNAGC	1 cut(s)	455
BlsI	GCNGC	3 cut(s)	15, 126, 283
BmcAI	AGTACT	1 cut(s)	168
Bme1390I	CCNGG	2 cut(s)	451, 516
Bme18I	GGWCC	1 cut(s)	556
BmgBI	CACGTC	2 cut(s)	439, 574
BmgT120I	GGNCC	1 cut(s)	556
BmiI	GGNNCC	1 cut(s)	384
BmrFI	CCNGG	2 cut(s)	451, 516
BmsI	GCATC	1 cut(s)	394
BpiI	GAAGAC	3 cut(s)	180, 540, 542
Bpu10I	CCTNAGC	1 cut(s)	627
Bpu1102I	GCTNAGC	1 cut(s)	455
Bpu14I	TTCGAA	1 cut(s)	195
BpuMI	CCSGG	1 cut(s)	516
Bsa29I	ATCGAT	1 cut(s)	336
BsaHI	GRCGYC	1 cut(s)	539
BsaJI	CCNNGG	1 cut(s)	542
Bsc4I	CCNNNNNNNGG	1 cut(s)	374
Bse1I	ACTGG	1 cut(s)	164
BseBI	CCWGG	1 cut(s)	451
BseCI	ATCGAT	1 cut(s)	336
BseDI	CCNNGG	1 cut(s)	542
BseGI	GGATG	1 cut(s)	409
BseLI	CCNNNNNNNGG	1 cut(s)	374
BseMII	CTCAG	2 cut(s)	446, 618
BseNI	ACTGG	1 cut(s)	164
BseRI	GAGGAG	1 cut(s)	605
BseXI	GCAGC	2 cut(s)	136, 268
Bsh1236I	CGCG	1 cut(s)	48
Bsh1285I	CGRYCG	1 cut(s)	565
BshFI	GGCC	1 cut(s)	607
BshVI	ATCGAT	1 cut(s)	336
BsiEI	CGRYCG	1 cut(s)	565
BsiHKAI	GWGCWC	1 cut(s)	9
BsiSI	CCGG	1 cut(s)	516
BslFI	GGGAC	2 cut(s)	77, 258
BslI	CCNNNNNNNGG	1 cut(s)	374
BsmAI	GTCTC	1 cut(s)	65
BsmFI	GGGAC	2 cut(s)	77, 258
BsnI	GGCC	1 cut(s)	607
Bsp119I	TTCGAA	1 cut(s)	195
Bsp1286I	GDGCHC	1 cut(s)	9
Bsp143I	GATC	5 cut(s)	100, 250, 337, 388, 562
Bsp1720I	GCTNAGC	1 cut(s)	455
BspANI	GGCC	1 cut(s)	607
BspCNI	CTCAG	2 cut(s)	447, 619
BspDI	ATCGAT	1 cut(s)	336
BspFNI	CGCG	1 cut(s)	48
BspHI	TCATGA	1 cut(s)	531
BspLI	GGNNCC	1 cut(s)	384
BspPI	GGATC	1 cut(s)	332
BspT104I	TTCGAA	1 cut(s)	195
BsrI	ACTGG	1 cut(s)	164
BssECI	CCNNGG	1 cut(s)	542
BssMI	GATC	5 cut(s)	100, 250, 337, 388, 562
BssNI	GRCGYC	1 cut(s)	539
BssT1I	CCWWGG	1 cut(s)	542
Bst2UI	CCWGG	1 cut(s)	451
Bst4CI	ACNGT	1 cut(s)	397
Bst6I	CTCTTC	1 cut(s)	110
BstACI	GRCGYC	1 cut(s)	539
BstBI	TTCGAA	1 cut(s)	195
BstC8I	GCNNGC	3 cut(s)	46, 112, 609
BstDEI	CTNAG	2 cut(s)	455, 627
BstENI	CCTNNNNNAGG	1 cut(s)	372
BstF5I	GGATG	1 cut(s)	409
BstFNI	CGCG	1 cut(s)	48
BstKTI	GATC	5 cut(s)	103, 253, 340, 391, 565
BstMAI	GTCTC	1 cut(s)	65
BstMBI	GATC	5 cut(s)	100, 250, 337, 388, 562
BstMCI	CGRYCG	1 cut(s)	565
BstMWI	GCNNNNNNNGC	3 cut(s)	13, 212, 221
BstNI	CCWGG	1 cut(s)	451
BstSCI	CCNGG	2 cut(s)	449, 514
BstSFI	CTRYAG	1 cut(s)	487
BstUI	CGCG	1 cut(s)	48
BstV1I	GCAGC	2 cut(s)	136, 268
BstV2I	GAAGAC	3 cut(s)	180, 540, 542
BstXI	CCANNNNNNTGG	1 cut(s)	622
Bsu15I	ATCGAT	1 cut(s)	336
BsuRI	GGCC	1 cut(s)	607
BsuTUI	ATCGAT	1 cut(s)	336
BtrI	CACGTC	2 cut(s)	439, 574
BtsCI	GGATG	1 cut(s)	409
BtsIMutI	CAGTG	1 cut(s)	402
Cac8I	GCNNGC	3 cut(s)	46, 112, 609
CciI	TCATGA	1 cut(s)	531
Cfr13I	GGNCC	1 cut(s)	556
ClaI	ATCGAT	1 cut(s)	336
CseI	GACGC	2 cut(s)	37, 547
Csp6I	GTAC	2 cut(s)	167, 442
CviAII	CATG	4 cut(s)	17, 65, 248, 532
CviQI	GTAC	2 cut(s)	167, 442
DdeI	CTNAG	2 cut(s)	455, 627
DpnI	GATC	5 cut(s)	102, 252, 339, 390, 564
DpnII	GATC	5 cut(s)	100, 250, 337, 388, 562
DrdI	GACNNNNNNGTC	1 cut(s)	544
DseDI	GACNNNNNNGTC	1 cut(s)	544
Eam1104I	CTCTTC	1 cut(s)	110
EarI	CTCTTC	1 cut(s)	110
Eco130I	CCWWGG	1 cut(s)	542
Eco147I	AGGCCT	1 cut(s)	607
Eco47I	GGWCC	1 cut(s)	556
Eco57I	CTGAAG	1 cut(s)	443
EcoNI	CCTNNNNNAGG	1 cut(s)	372
EcoRII	CCWGG	1 cut(s)	449
EcoT14I	CCWWGG	1 cut(s)	542
ErhI	CCWWGG	1 cut(s)	542
FaeI	CATG	4 cut(s)	20, 68, 251, 535
FaiI	YATR	8 cut(s)	18, 66, 217, 219, 249, 298, 533, 635
FalI	AAGNNNNNCTT	2 cut(s)	134, 166
FaqI	GGGAC	2 cut(s)	77, 258
FatI	CATG	4 cut(s)	16, 64, 247, 531
FauNDI	CATATG	1 cut(s)	217
Fnu4HI	GCNGC	3 cut(s)	14, 125, 282
FokI	GGATG	1 cut(s)	416
Fsp4HI	GCNGC	3 cut(s)	14, 125, 282
FspBI	CTAG	4 cut(s)	170, 201, 494, 603
GluI	GCNGC	3 cut(s)	14, 125, 282
HaeIII	GGCC	1 cut(s)	607
HapII	CCGG	1 cut(s)	516
HgaI	GACGC	2 cut(s)	37, 547
Hin1I	GRCGYC	1 cut(s)	539
Hin1II	CATG	4 cut(s)	20, 68, 251, 535
HinfI	GANTC	3 cut(s)	145, 197, 311
HpaII	CCGG	1 cut(s)	516
HphI	GGTGA	3 cut(s)	301, 491, 497
Hpy166II	GTNNAC	2 cut(s)	442, 559
Hpy188III	TCNNGA	2 cut(s)	263, 532
Hpy8I	GTNNAC	2 cut(s)	442, 559
Hpy99I	CGWCG	2 cut(s)	53, 56
HpyAV	CCTTC	3 cut(s)	286, 421, 512
HpyCH4III	ACNGT	1 cut(s)	397
HpyCH4IV	ACGT	2 cut(s)	438, 573
HpyCH4V	TGCA	4 cut(s)	16, 284, 332, 407
HpyF10VI	GCNNNNNNNGC	3 cut(s)	13, 212, 221
HpyF3I	CTNAG	2 cut(s)	455, 627
HpySE526I	ACGT	2 cut(s)	438, 573
Hsp92I	GRCGYC	1 cut(s)	539
Hsp92II	CATG	4 cut(s)	20, 68, 251, 535
Kzo9I	GATC	5 cut(s)	100, 250, 337, 388, 562
Lsp1109I	GCAGC	2 cut(s)	136, 268
LweI	GCATC	1 cut(s)	394
MaeI	CTAG	4 cut(s)	170, 201, 494, 603
MaeII	ACGT	2 cut(s)	438, 573
MaeIII	GTNAC	1 cut(s)	260
MalI	GATC	5 cut(s)	102, 252, 339, 390, 564
MboI	GATC	5 cut(s)	100, 250, 337, 388, 562
MboII	GAAGA	5 cut(s)	97, 185, 436, 540, 547
MhlI	GDGCHC	1 cut(s)	9
MluCI	AATT	4 cut(s)	131, 230, 342, 637
MnlI	CCTC	5 cut(s)	271, 579, 583, 622, 622
MspI	CCGG	1 cut(s)	516
MspR9I	CCNGG	2 cut(s)	451, 516
MvaI	CCWGG	1 cut(s)	451
MvnI	CGCG	1 cut(s)	48
MwoI	GCNNNNNNNGC	3 cut(s)	13, 212, 221
NciI	CCSGG	1 cut(s)	516
NdeI	CATATG	1 cut(s)	217
NdeII	GATC	5 cut(s)	100, 250, 337, 388, 562
NlaIII	CATG	4 cut(s)	20, 68, 251, 535
NlaIV	GGNNCC	1 cut(s)	384
NmuCI	GTSAC	1 cut(s)	260
NspV	TTCGAA	1 cut(s)	195
PagI	TCATGA	1 cut(s)	531
PceI	AGGCCT	1 cut(s)	607
PfeI	GAWTC	3 cut(s)	145, 197, 311
PkrI	GCNGC	3 cut(s)	15, 126, 283
Ple19I	CGATCG	1 cut(s)	565
Psp6I	CCWGG	1 cut(s)	449
PspGI	CCWGG	1 cut(s)	449
PspN4I	GGNNCC	1 cut(s)	384
PspPI	GGNCC	1 cut(s)	556
PvuI	CGATCG	1 cut(s)	565
RsaI	GTAC	2 cut(s)	168, 443
RsaNI	GTAC	2 cut(s)	167, 442
SatI	GCNGC	3 cut(s)	14, 125, 282
Sau3AI	GATC	5 cut(s)	100, 250, 337, 388, 562
Sau96I	GGNCC	1 cut(s)	556
ScaI	AGTACT	1 cut(s)	168
ScrFI	CCNGG	2 cut(s)	451, 516
SduI	GDGCHC	1 cut(s)	9
SfaNI	GCATC	1 cut(s)	394
SfcI	CTRYAG	1 cut(s)	487
SfuI	TTCGAA	1 cut(s)	195
SinI	GGWCC	1 cut(s)	556
SmlI	CTYRAG	1 cut(s)	8
SmoI	CTYRAG	1 cut(s)	8
Sse9I	AATT	4 cut(s)	131, 230, 342, 637
SseBI	AGGCCT	1 cut(s)	607
SspMI	CTAG	4 cut(s)	170, 201, 494, 603
StuI	AGGCCT	1 cut(s)	607
StyD4I	CCNGG	2 cut(s)	449, 514
StyI	CCWWGG	1 cut(s)	542
TaaI	ACNGT	1 cut(s)	397
TaiI	ACGT	2 cut(s)	441, 576
TaqI	TCGA	4 cut(s)	84, 195, 336, 351
TasI	AATT	4 cut(s)	131, 230, 342, 637
TatI	WGTACW	1 cut(s)	166
TfiI	GAWTC	3 cut(s)	145, 197, 311
TscAI	CASTG	1 cut(s)	402
TseFI	GTSAC	1 cut(s)	260
TseI	GCWGC	3 cut(s)	13, 124, 281
Tsp45I	GTSAC	1 cut(s)	260
TspDTI	ATGAA	5 cut(s)	81, 520, 548, 588, 650
TspRI	CASTG	1 cut(s)	402
VpaK11BI	GGWCC	1 cut(s)	556
XagI	CCTNNNNNAGG	1 cut(s)	372
XapI	RAATTY	3 cut(s)	131, 230, 637
XspI	CTAG	4 cut(s)	170, 201, 494, 603
ZrmI	AGTACT	1 cut(s)	168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.