FvH4_2g28060

tetratricopeptide repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008353
Physical Location & Seq
Reverse (-)
22129593 .. 22140298
10706 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28060.t4

Sequence Viewer

Length: 657 bp
ATGAAACTAGCGTGGAAGAACAACAACAAGAAGCGGCCTCTGGCCACCGTCTCCAAGTACCCAAACCTCCCTTTTGATCACAAAAACAACAACCCCACTGCCCAAAACGACACACCCGACAAACCCAATGGTGATCAAAACGACACCGAGCTAAACAAGGAACAACACCCTGAACCGAACGATCCCTCAGTCTCTGGAGAAGAAGACGAAGACGTTACCAAACTCTTCACCTCGTTTCAGTCCCAGGGAACTAAGCTAGCCGAGGATGGGAAGTATCGCGAAGCATTGGGAAAATGGGAGTCTGCTCTTATGTTGATGCCCAAAAGTGCAGTTTTACACGAGCAGAAGGCGCAAGTTCTGATTGAAATTGGTGATGCGTGGAATGCAATAAAGGCAGCAACTCGAGCTACTGAGTTGGAGCCTTCATGGGCTGAGGCATGGGTTACCCTTGGCAGAGCTCAGTTAAACTTTGGGGAACCTGATAGTGCCGTCCAAAGCTTTGACAAAGCACTAGCCATCAAGCCTGATTCTGAGGAGGCTCGAGATGACAGACATACTGCGATGAATCTCCTGAAAAGGCGAAAACAGCTCCATTCATCAGGCTTAAGTCCTACAAGAAACCGTTATGCAGTTGGGGATAGCATTGATAGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC4 TPR_11 TPR_14 TPR_16 TPR_19 TPR_2 TPR_8

Protein Analysis

219

Amino Acids

24.42

Weight (kDa)

5.8

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 279
AciI CCGC 1 cut(s) 34
AclWI GGATC 1 cut(s) 176
AcoI YGGCCR 1 cut(s) 42
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 267
AflII CTTAAG 1 cut(s) 604
AgsI TTSAA 1 cut(s) 365
AjnI CCWGG 1 cut(s) 243
AluBI AGCT 6 cut(s) 151, 256, 407, 458, 498, 589
AluI AGCT 6 cut(s) 151, 256, 407, 458, 498, 589
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 2 cut(s) 55, 196
AlwI GGATC 1 cut(s) 176
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 2 cut(s) 402, 540
AoxI GGCC 2 cut(s) 35, 42
ApeKI GCWGC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 352
AsuHPI GGTGA 3 cut(s) 143, 220, 383
AsuNHI GCTAGC 1 cut(s) 256
AvaI CYCGRG 2 cut(s) 402, 540
BalI TGGCCA 1 cut(s) 44
BanII GRGCYC 1 cut(s) 460
BauI CACGAG 1 cut(s) 338
BbsI GAAGAC 2 cut(s) 210, 216
Bbv12I GWGCWC 1 cut(s) 460
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 1 cut(s) 407
BccI CCATC 2 cut(s) 260, 524
BceAI ACGGC 1 cut(s) 473
BciT130I CCWGG 1 cut(s) 245
BclI TGATCA 2 cut(s) 76, 133
BcoDI GTCTC 2 cut(s) 55, 196
BfaI CTAG 3 cut(s) 8, 257, 512
BfrI CTTAAG 1 cut(s) 604
BisI GCNGC 2 cut(s) 35, 396
BlsI GCNGC 2 cut(s) 36, 397
Bme1390I CCNGG 1 cut(s) 245
BmeT110I CYCGRG 2 cut(s) 402, 540
BmiI GGNNCC 2 cut(s) 420, 477
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 2 cut(s) 306, 364
BmtI GCTAGC 1 cut(s) 260
BpiI GAAGAC 2 cut(s) 210, 216
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 432
BsaJI CCNNGG 4 cut(s) 243, 244, 261, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 267
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 4 cut(s) 243, 244, 261, 448
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMII CTCAG 5 cut(s) 201, 402, 423, 473, 522
BseRI GAGGAG 1 cut(s) 548
BseXI GCAGC 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 2 cut(s) 37, 44
BsiHKAI GWGCWC 1 cut(s) 460
BsiHKCI CYCGRG 2 cut(s) 402, 540
BslFI GGGAC 1 cut(s) 226
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 2 cut(s) 55, 196
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 226
BsmI GAATGC 1 cut(s) 388
BsnI GGCC 2 cut(s) 37, 44
BsoBI CYCGRG 2 cut(s) 402, 540
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 3 cut(s) 76, 133, 181
Bsp68I TCGCGA 1 cut(s) 279
BspACI CCGC 1 cut(s) 34
BspANI GGCC 2 cut(s) 37, 44
BspCNI CTCAG 5 cut(s) 200, 403, 424, 472, 523
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 2 cut(s) 420, 477
BspOI GCTAGC 1 cut(s) 260
BspPI GGATC 1 cut(s) 176
BspTI CTTAAG 1 cut(s) 604
BssECI CCNNGG 4 cut(s) 243, 244, 261, 448
BssMI GATC 3 cut(s) 76, 133, 181
BssSI CACGAG 1 cut(s) 338
BssT1I CCWWGG 1 cut(s) 448
Bst2BI CACGAG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 2 cut(s) 49, 623
Bst6I CTCTTC 1 cut(s) 230
BstAFI CTTAAG 1 cut(s) 604
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 6 cut(s) 187, 252, 411, 432, 459, 531
BstEII GGTNACC 1 cut(s) 442
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 3 cut(s) 79, 136, 184
BstMAI GTCTC 2 cut(s) 55, 196
BstMBI GATC 3 cut(s) 76, 133, 181
BstMWI GCNNNNNNNGC 5 cut(s) 349, 383, 392, 404, 586
BstNI CCWGG 1 cut(s) 245
BstPI GGTNACC 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 243
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 1 cut(s) 407
BstV2I GAAGAC 2 cut(s) 210, 216
BsuRI GGCC 2 cut(s) 37, 44
BtgZI GCGATG 1 cut(s) 575
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
BtuMI TCGCGA 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 258
CaiI CAGNNNCTG 1 cut(s) 194
CfoI GCGC 1 cut(s) 352
Csp6I GTAC 1 cut(s) 58
CviAII CATG 3 cut(s) 426, 438, 654
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 6 cut(s) 187, 252, 411, 432, 459, 531
DpnI GATC 3 cut(s) 78, 135, 183
DpnII GATC 3 cut(s) 76, 133, 181
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 230
EarI CTCTTC 1 cut(s) 230
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 458
Eco88I CYCGRG 2 cut(s) 402, 540
Eco91I GGTNACC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 458
EcoO65I GGTNACC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 448
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 3 cut(s) 429, 441, 657
FaiI YATR 6 cut(s) 311, 427, 439, 555, 627, 655
FaqI GGGAC 1 cut(s) 226
FatI CATG 3 cut(s) 425, 437, 653
FbaI TGATCA 2 cut(s) 76, 133
Fnu4HI GCNGC 2 cut(s) 35, 396
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 2 cut(s) 35, 396
FspBI CTAG 3 cut(s) 8, 257, 512
GlaI GCGC 1 cut(s) 351
GluI GCNGC 2 cut(s) 35, 396
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 2 cut(s) 37, 44
HhaI GCGC 1 cut(s) 352
Hin1II CATG 3 cut(s) 429, 441, 657
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 3 cut(s) 299, 527, 565
HphI GGTGA 3 cut(s) 143, 220, 383
Hpy188I TCNGA 2 cut(s) 360, 532
Hpy188III TCNNGA 4 cut(s) 195, 278, 542, 571
HpyAV CCTTC 2 cut(s) 340, 432
HpyCH4III ACNGT 2 cut(s) 49, 623
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 3 cut(s) 329, 386, 629
HpyF10VI GCNNNNNNNGC 5 cut(s) 349, 383, 392, 404, 586
HpyF3I CTNAG 6 cut(s) 187, 252, 411, 432, 459, 531
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 3 cut(s) 429, 441, 657
HspAI GCGC 1 cut(s) 350
Ksp22I TGATCA 2 cut(s) 76, 133
Kzo9I GATC 3 cut(s) 76, 133, 181
LmnI GCTCC 2 cut(s) 418, 594
LpnPI CCDG 9 cut(s) 26, 180, 183, 230, 257, 492, 537, 584, 585
Lsp1109I GCAGC 1 cut(s) 407
LweI GCATC 2 cut(s) 306, 364
MaeI CTAG 3 cut(s) 8, 257, 512
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 214, 442
MalI GATC 3 cut(s) 78, 135, 183
MboI GATC 3 cut(s) 76, 133, 181
MboII GAAGA 5 cut(s) 28, 212, 215, 217, 221
MhlI GDGCHC 1 cut(s) 460
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 1 cut(s) 366
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 8 cut(s) 48, 77, 196, 241, 256, 427, 526, 529
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 464, 605
Msp20I TGGCCA 1 cut(s) 44
MspCI CTTAAG 1 cut(s) 604
MspR9I CCNGG 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 388
MvaI CCWGG 1 cut(s) 245
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 5 cut(s) 349, 383, 392, 404, 586
NdeII GATC 3 cut(s) 76, 133, 181
NheI GCTAGC 1 cut(s) 256
NlaIII CATG 3 cut(s) 429, 441, 657
NlaIV GGNNCC 2 cut(s) 420, 477
NmeAIII GCCGAG 1 cut(s) 286
NruI TCGCGA 1 cut(s) 279
PaeR7I CTCGAG 2 cut(s) 402, 540
PasI CCCWGGG 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 388
PfeI GAWTC 2 cut(s) 527, 565
PkrI GCNGC 2 cut(s) 36, 397
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 243
PspEI GGTNACC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 420, 477
PspXI VCTCGAGB 1 cut(s) 402
PstNI CAGNNNCTG 1 cut(s) 194
RruI TCGCGA 1 cut(s) 279
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SacI GAGCTC 1 cut(s) 460
SaqAI TTAA 2 cut(s) 464, 605
SatI GCNGC 2 cut(s) 35, 396
Sau3AI GATC 3 cut(s) 76, 133, 181
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 2 cut(s) 306, 364
Sfr274I CTCGAG 2 cut(s) 402, 540
SlaI CTCGAG 2 cut(s) 402, 540
SmlI CTYRAG 3 cut(s) 402, 540, 604
SmoI CTYRAG 3 cut(s) 402, 540, 604
Sse9I AATT 1 cut(s) 366
SsiI CCGC 1 cut(s) 34
SspMI CTAG 3 cut(s) 8, 257, 512
SstI GAGCTC 1 cut(s) 460
StyD4I CCNGG 1 cut(s) 243
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 2 cut(s) 49, 623
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 403, 541
TasI AATT 1 cut(s) 366
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 2 cut(s) 527, 565
Tru1I TTAA 2 cut(s) 464, 605
Tru9I TTAA 2 cut(s) 464, 605
TscAI CASTG 1 cut(s) 103
TseI GCWGC 1 cut(s) 395
TspDTI ATGAA 4 cut(s) 17, 414, 578, 585
TspRI CASTG 1 cut(s) 103
Vha464I CTTAAG 1 cut(s) 604
XhoI CTCGAG 2 cut(s) 402, 540
XspI CTAG 3 cut(s) 8, 257, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.