MD15G1131600.v1.1

tetratricopeptide repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008353
Physical Location & Seq
Forward (+)
9539170 .. 9542599
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1131600.v1.1.491

Sequence Viewer

Length: 621 bp
ATGAAGATAGCGTGGAAGAACAACAAGAAGCGGCCTCTGGGGATCGTATCAACTTACCCAAATCTCCCTTTCGAGCGGCAAAACGACGAACCCAGCAATCAACCGCACCCAGCAGAAAAGCCCAGCAATAATATTCAAAACAACACAGAGCTCGGCAAGCTAGCCGATGAGAGCGAGCCTTCCGACGCTGCTGAACTCTTCCACTCCTTTCAAGCTCAGGGAACTAAGCTCGCCGAGGATGGGAAGTACCGTGAAGCATTGGGAAAATGGGAGGCTGCTCTTATGTTGAGGCCTGAAAATGCAGTTTTACATGAACAGAAGGCACAAGTATTACTTGAAGTTGGAGATGCATGGAATGCGGTGAAGACAGCGACTCGAGCTACTGAGTTGGAGCCTTCATGGGCTGAGGCTTGGGTTACCCTTGGTAGAGCACAGTTAAACTTTGGGGAGCCTGATAGTTCCATTCAAAGCTTCGACCGAGCATTAGCCATCAAGCCTGATTCTGAGGAGGCTCGAAATGACAGACATACTGCAATGCAACTGGTGACAAAGCGAAAACAGCTCCATTCATCAGGTTTAAGTCCTACGAGGAAACGTTATGCAGTTGGTGATAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC4 TPR_11 TPR_14 TPR_16 TPR_19 TPR_2 TPR_8

Protein Analysis

207

Amino Acids

23.02

Weight (kDa)

6.54

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_16 PF13432 107 - 168 2.6e-06 Tetratricopeptide repeat
TPR_CcmH_CycH PF23914 131 - 179 8.2e-06 Cytochrome c-type biogenesis protein H TPR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 76
AciI CCGC 4 cut(s) 31, 76, 104, 359
AclI AACGTT 1 cut(s) 595
AclWI GGATC 1 cut(s) 50
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 1 cut(s) 240
AgsI TTSAA 4 cut(s) 137, 212, 338, 467
AluBI AGCT 7 cut(s) 151, 160, 215, 229, 380, 471, 562
AluI AGCT 7 cut(s) 151, 160, 215, 229, 380, 471, 562
Alw21I GWGCWC 2 cut(s) 153, 433
AlwI GGATC 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 375
AoxI GGCC 2 cut(s) 32, 290
ApeKI GCWGC 2 cut(s) 188, 275
AsuHPI GGTGA 3 cut(s) 373, 556, 620
AsuNHI GCTAGC 1 cut(s) 160
AvaI CYCGRG 1 cut(s) 375
BanII GRGCYC 1 cut(s) 153
BbsI GAAGAC 1 cut(s) 371
Bbv12I GWGCWC 2 cut(s) 153, 433
BbvCI CCTCAGC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 175, 262
BccI CCATC 2 cut(s) 233, 497
BfaI CTAG 1 cut(s) 161
BisI GCNGC 4 cut(s) 32, 77, 189, 276
BlsI GCNGC 4 cut(s) 33, 78, 190, 277
BmeT110I CYCGRG 1 cut(s) 375
BmiI GGNNCC 2 cut(s) 393, 450
BmsI GCATC 1 cut(s) 337
BmtI GCTAGC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 371
Bpu10I CCTNAGC 2 cut(s) 216, 405
BsaJI CCNNGG 2 cut(s) 234, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 234, 421
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 240
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 4 cut(s) 230, 375, 396, 495
BseNI ACTGG 1 cut(s) 546
BseRI GAGGAG 1 cut(s) 521
BseXI GCAGC 2 cut(s) 175, 262
BseYI CCCAGC 3 cut(s) 92, 109, 122
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 2 cut(s) 34, 292
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 2 cut(s) 153, 433
BsiHKCI CYCGRG 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 240
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 2 cut(s) 34, 292
BsoBI CYCGRG 1 cut(s) 375
Bsp1286I GDGCHC 2 cut(s) 153, 433
Bsp143I GATC 1 cut(s) 42
BspACI CCGC 4 cut(s) 31, 76, 104, 359
BspANI GGCC 2 cut(s) 34, 292
BspCNI CTCAG 4 cut(s) 229, 376, 397, 496
BspLI GGNNCC 2 cut(s) 393, 450
BspOI GCTAGC 1 cut(s) 164
BspPI GGATC 1 cut(s) 50
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 1 cut(s) 546
BssECI CCNNGG 2 cut(s) 234, 421
BssMI GATC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 421
Bst4CI ACNGT 2 cut(s) 251, 435
Bst6I CTCTTC 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 4 cut(s) 158, 162, 176, 231
BstDEI CTNAG 5 cut(s) 216, 225, 384, 405, 504
BstEII GGTNACC 1 cut(s) 415
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 1 cut(s) 45
BstMBI GATC 1 cut(s) 42
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 4 cut(s) 157, 356, 377, 559
BstPI GGTNACC 1 cut(s) 415
BstV1I GCAGC 2 cut(s) 175, 262
BstV2I GAAGAC 1 cut(s) 371
BsuRI GGCC 2 cut(s) 34, 292
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 4 cut(s) 158, 162, 176, 231
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 247
CviAII CATG 4 cut(s) 311, 351, 399, 618
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 5 cut(s) 216, 225, 384, 405, 504
DpnI GATC 1 cut(s) 44
DpnII GATC 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Ecl136II GAGCTC 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 421
Eco147I AGGCCT 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 153
Eco53kI GAGCTC 1 cut(s) 151
Eco88I CYCGRG 1 cut(s) 375
Eco91I GGTNACC 1 cut(s) 415
EcoICRI GAGCTC 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 415
EcoT14I CCWWGG 1 cut(s) 421
EcoT22I ATGCAT 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 421
FaeI CATG 4 cut(s) 314, 354, 402, 621
FaiI YATR 7 cut(s) 284, 312, 352, 400, 528, 600, 619
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FatI CATG 4 cut(s) 310, 350, 398, 617
Fnu4HI GCNGC 4 cut(s) 32, 77, 189, 276
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 4 cut(s) 32, 77, 189, 276
FspBI CTAG 1 cut(s) 161
GluI GCNGC 4 cut(s) 32, 77, 189, 276
GsaI CCCAGC 3 cut(s) 96, 113, 126
HaeIII GGCC 2 cut(s) 34, 292
HgaI GACGC 1 cut(s) 194
Hin1II CATG 4 cut(s) 314, 354, 402, 621
HindIII AAGCTT 1 cut(s) 469
HinfI GANTC 2 cut(s) 373, 500
HphI GGTGA 3 cut(s) 373, 556, 620
Hpy188I TCNGA 2 cut(s) 184, 505
Hpy99I CGWCG 2 cut(s) 89, 188
HpyAV CCTTC 3 cut(s) 189, 313, 405
HpyCH4III ACNGT 2 cut(s) 251, 435
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 5 cut(s) 302, 350, 533, 538, 602
HpyF10VI GCNNNNNNNGC 4 cut(s) 157, 356, 377, 559
HpyF3I CTNAG 5 cut(s) 216, 225, 384, 405, 504
HpySE526I ACGT 1 cut(s) 595
Hsp92II CATG 4 cut(s) 314, 354, 402, 621
Kzo9I GATC 1 cut(s) 42
LmnI GCTCC 3 cut(s) 391, 448, 567
Lsp1109I GCAGC 2 cut(s) 175, 262
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 2 cut(s) 415, 544
MalI GATC 1 cut(s) 44
MbiI CCGCTC 1 cut(s) 76
MboI GATC 1 cut(s) 42
MboII GAAGA 4 cut(s) 16, 28, 190, 376
MhlI GDGCHC 2 cut(s) 153, 433
MlyI GAGTC 1 cut(s) 367
MmeI TCCRAC 3 cut(s) 207, 322, 369
MnlI CCTC 8 cut(s) 45, 229, 265, 282, 400, 499, 502, 582
Mph1103I ATGCAT 1 cut(s) 352
MseI TTAA 2 cut(s) 437, 578
Mva1269I GAATGC 1 cut(s) 361
MwoI GCNNNNNNNGC 4 cut(s) 157, 356, 377, 559
NdeII GATC 1 cut(s) 42
NheI GCTAGC 1 cut(s) 160
NlaIII CATG 4 cut(s) 314, 354, 402, 621
NlaIV GGNNCC 2 cut(s) 393, 450
NmeAIII GCCGAG 2 cut(s) 132, 259
NmuCI GTSAC 1 cut(s) 544
NsiI ATGCAT 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 375
PceI AGGCCT 1 cut(s) 292
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 4 cut(s) 33, 78, 190, 277
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 153
Psp1406I AACGTT 1 cut(s) 595
PspEI GGTNACC 1 cut(s) 415
PspFI CCCAGC 3 cut(s) 92, 109, 122
PspN4I GGNNCC 2 cut(s) 393, 450
PspXI VCTCGAGB 1 cut(s) 375
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 2 cut(s) 437, 578
SatI GCNGC 4 cut(s) 32, 77, 189, 276
Sau3AI GATC 1 cut(s) 42
SchI GAGTC 1 cut(s) 367
SduI GDGCHC 2 cut(s) 153, 433
SetI ASST 9 cut(s) 153, 162, 217, 231, 382, 473, 564, 577, 598
SfaNI GCATC 1 cut(s) 337
Sfr274I CTCGAG 1 cut(s) 375
SlaI CTCGAG 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 375
SmoI CTYRAG 1 cut(s) 375
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 4 cut(s) 31, 76, 104, 359
SspI AATATT 1 cut(s) 133
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 153
StuI AGGCCT 1 cut(s) 292
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 2 cut(s) 251, 435
TaiI ACGT 1 cut(s) 598
TaqI TCGA 4 cut(s) 72, 376, 474, 514
TaqII GACCGA 1 cut(s) 492
TauI GCSGC 2 cut(s) 34, 79
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 2 cut(s) 437, 578
Tru9I TTAA 2 cut(s) 437, 578
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 2 cut(s) 188, 275
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 4 cut(s) 17, 327, 387, 558
XhoI CTCGAG 1 cut(s) 375
XspI CTAG 1 cut(s) 161
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.