FvH4_2g31770

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008843
Physical Location & Seq
Reverse (-)
24284429 .. 24285334
906 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31770.t1

Sequence Viewer

Length: 507 bp
ATGAAGAAGCACCAGACTTGTGCTGCTTGCAGATTCAAGCGCTGCAAATGTGGACCTGATTGTCCAATGGCCCCGTACTTCCCTGCCCACATGCACCATCACTACAAGAATGCCCACAAGGTGTTCGGCGAAAGTCACATCAAGAAGTTGCTCCGCAGCGTACCCCCTCACAACAGAAGCACTGCCATGAAGCACATTATAGCCGAAGCTGGCATCCGCGTCGCCGACCCGGTTGGTGGAAGCTACGGTCTTATTAGGAACTTGCAGCTCAAGATTGCCGAGGAAGAGGAGAACCTCCGCCGCTTGCGAGATGTACTCAGCGTGTTTTGTGGCGGAGGGAGTGGTGATGGTGTTGTGGCACAGGGTGTGGTTCAGAAACAGGGCTGCATTGTTCCTGGTGGATTTCTTCATGCACTCTTTCTTCAAGAACAGGGGAACAACATGTGTGTAAGGGATGAAGTTGTGCCGAAACATGATGTCGAATCAATTCAGGGTGAGCAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

169

Amino Acids

18.56

Weight (kDa)

8.95

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 7 - 102 4.1e-30 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 60
AccII CGCG 1 cut(s) 219
AciI CCGC 5 cut(s) 154, 217, 298, 301, 333
AdeI CACNNNGTG 2 cut(s) 121, 365
AfaI GTAC 3 cut(s) 77, 162, 315
AfeI AGCGCT 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 236
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 2 cut(s) 37, 425
AjnI CCWGG 1 cut(s) 394
AluBI AGCT 3 cut(s) 209, 243, 268
AluI AGCT 3 cut(s) 209, 243, 268
Aor51HI AGCGCT 1 cut(s) 41
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 5 cut(s) 23, 42, 156, 265, 384
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 2 cut(s) 53, 70
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 356, 506
AvaII GGWCC 1 cut(s) 53
BbvI GCAGC 5 cut(s) 10, 29, 168, 277, 371
BccI CCATC 2 cut(s) 105, 341
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BciT130I CCWGG 1 cut(s) 396
BcnI CCSGG 1 cut(s) 230
BfoI RGCGCY 1 cut(s) 43
BisI GCNGC 6 cut(s) 24, 43, 157, 266, 301, 385
BlsI GCNGC 6 cut(s) 25, 44, 158, 267, 302, 386
Bme1390I CCNGG 2 cut(s) 230, 396
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 53, 70
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 230, 396
BmsI GCATC 1 cut(s) 222
BplI GAGNNNNNCTC 2 cut(s) 300, 332
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 1 cut(s) 236
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 2 cut(s) 213, 460
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMII CTCAG 1 cut(s) 331
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 5 cut(s) 10, 29, 168, 277, 371
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 236
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 1 cut(s) 71
BspACI CCGC 5 cut(s) 154, 217, 298, 301, 333
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 330
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 279
BstC8I GCNNGC 3 cut(s) 28, 211, 305
BstDEI CTNAG 1 cut(s) 317
BstF5I GGATG 2 cut(s) 213, 460
BstFNI CGCG 1 cut(s) 219
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 1 cut(s) 42
BstNI CCWGG 1 cut(s) 396
BstNSI RCATGY 2 cut(s) 94, 445
BstSCI CCNGG 2 cut(s) 228, 394
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 5 cut(s) 10, 29, 168, 277, 371
BsuRI GGCC 1 cut(s) 71
BtsCI GGATG 2 cut(s) 213, 460
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 3 cut(s) 28, 211, 305
CfoI GCGC 1 cut(s) 42
Cfr13I GGNCC 2 cut(s) 53, 70
CseI GACGC 1 cut(s) 208
Csp6I GTAC 3 cut(s) 76, 161, 314
CviAII CATG 5 cut(s) 91, 187, 410, 442, 473
CviJI RGCY 6 cut(s) 71, 203, 209, 243, 268, 384
CviKI_1 RGCY 6 cut(s) 71, 203, 209, 243, 268, 384
CviQI GTAC 3 cut(s) 76, 161, 314
DdeI CTNAG 1 cut(s) 317
DraIII CACNNNGTG 2 cut(s) 121, 365
DrdI GACNNNNNNGTC 1 cut(s) 60
DseDI GACNNNNNNGTC 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EciI GGCGGA 2 cut(s) 287, 348
Eco47I GGWCC 1 cut(s) 53
Eco47III AGCGCT 1 cut(s) 41
EcoRII CCWGG 1 cut(s) 394
FaeI CATG 5 cut(s) 94, 190, 413, 445, 476
FaiI YATR 6 cut(s) 92, 188, 200, 411, 443, 474
FatI CATG 5 cut(s) 90, 186, 409, 441, 472
Fnu4HI GCNGC 6 cut(s) 24, 43, 157, 266, 301, 385
FokI GGATG 2 cut(s) 200, 467
Fsp4HI GCNGC 6 cut(s) 24, 43, 157, 266, 301, 385
GlaI GCGC 1 cut(s) 41
GluI GCNGC 6 cut(s) 24, 43, 157, 266, 301, 385
HaeII RGCGCY 1 cut(s) 43
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 230
HgaI GACGC 1 cut(s) 208
HhaI GCGC 1 cut(s) 42
Hin1II CATG 5 cut(s) 94, 190, 413, 445, 476
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 2 cut(s) 33, 482
HpaII CCGG 1 cut(s) 230
HphI GGTGA 2 cut(s) 356, 506
Hpy166II GTNNAC 1 cut(s) 53
Hpy188I TCNGA 1 cut(s) 375
Hpy188III TCNNGA 3 cut(s) 142, 271, 425
Hpy8I GTNNAC 1 cut(s) 53
Hpy99I CGWCG 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 6 cut(s) 30, 45, 94, 265, 387, 413
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 5 cut(s) 94, 190, 413, 445, 476
HspAI GCGC 1 cut(s) 40
LmnI GCTCC 1 cut(s) 156
Lsp1109I GCAGC 5 cut(s) 10, 29, 168, 277, 371
LweI GCATC 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 134
MboII GAAGA 4 cut(s) 16, 296, 398, 413
MluCI AATT 2 cut(s) 486, 502
MnlI CCTC 5 cut(s) 177, 274, 280, 305, 329
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 1 cut(s) 505
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 2 cut(s) 230, 396
Mva1269I GAATGC 1 cut(s) 115
MvaI CCWGG 1 cut(s) 396
MvnI CGCG 1 cut(s) 219
NciI CCSGG 1 cut(s) 230
NlaIII CATG 5 cut(s) 94, 190, 413, 445, 476
NlaIV GGNNCC 1 cut(s) 72
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 1 cut(s) 134
NspI RCATGY 2 cut(s) 94, 445
PciI ACATGT 1 cut(s) 441
PctI GAATGC 1 cut(s) 115
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 2 cut(s) 33, 482
PkrI GCNGC 6 cut(s) 25, 44, 158, 267, 302, 386
PscI ACATGT 1 cut(s) 441
Psp6I CCWGG 1 cut(s) 394
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 53, 70
RsaI GTAC 3 cut(s) 77, 162, 315
RsaNI GTAC 3 cut(s) 76, 161, 314
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 1 cut(s) 505
SatI GCNGC 6 cut(s) 24, 43, 157, 266, 301, 385
Sau96I GGNCC 2 cut(s) 53, 70
ScrFI CCNGG 2 cut(s) 230, 396
SetI ASST 6 cut(s) 58, 123, 211, 245, 270, 297
SfaNI GCATC 1 cut(s) 222
SinI GGWCC 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 2 cut(s) 486, 502
SsiI CCGC 5 cut(s) 154, 217, 298, 301, 333
StyD4I CCNGG 2 cut(s) 228, 394
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 480
TasI AATT 2 cut(s) 486, 502
TatI WGTACW 1 cut(s) 313
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 2 cut(s) 33, 482
Tru1I TTAA 1 cut(s) 505
Tru9I TTAA 1 cut(s) 505
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 5 cut(s) 23, 42, 156, 265, 384
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 17, 203, 398, 471
TspRI CASTG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 53
XceI RCATGY 2 cut(s) 94, 445
XmnI GAANNNNTTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.