MD15G1058700.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008843
Physical Location & Seq
Forward (+)
3925673 .. 3927091
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1058700.v1.1.491

Sequence Viewer

Length: 546 bp
ATGAAGAAGTCCCCAAGTTGCGCCGCTTGTAAACACCAGCGCAAAAAATGCCCAGAGGACTGCCCCTTGGCCCCCTACTTCCCCTCCAACCGCCCCACAAACTTCCACAACGCACTCAAACTGTTCGGCGTCAGTAGACTCACCAAACTCGTCACCGACCTCCCTCCCCACCTCCGAACCCCCGCCATGTCCACCATCTTCGTCGAAGCCGACCACCGCGCAAAAGACCCTGTTGGCGGCTGCCACGCCGTCGTCCGCTCGCTGCTCTGGTCCATCAACAAGCACCAGGCCGAACTCGACCGCGTGCGCTACCAACTCGAATTGTGCCGCGGCGGCGGCAATGTTATGCCGATGGTTATGCCGATGGCGCAGAGTCTGAGGGACTTTAAGTTCAATGTGGGTGCGGGAAATGTGTACGATCAAACGCAGTTTCATCAGGTGCAGAAGAACGTTTTTCAAGAACAAGAAGAACAAGGCAATGTTGGTGGAGGAGTTGTTCCGCAGCCTCACGAAGAACAGAGCAATGTGTTTGATATAAGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

182

Amino Acids

20.32

Weight (kDa)

8.96

Isoelectric Point (pI)

59.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 6 - 102 2.7e-32 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 136
AccII CGCG 3 cut(s) 219, 303, 330
AclI AACGTT 1 cut(s) 450
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 2 cut(s) 394, 458
AjnI CCWGG 1 cut(s) 285
AloI GAACNNNNNNTCC 4 cut(s) 374, 406, 480, 512
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 3 cut(s) 69, 288, 540
ApeKI GCWGC 3 cut(s) 240, 262, 502
AspLEI GCGC 5 cut(s) 23, 42, 221, 309, 370
AspS9I GGNCC 3 cut(s) 70, 270, 540
AsuHPI GGTGA 2 cut(s) 133, 145
AvaII GGWCC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 227, 249, 514
BccI CCATC 4 cut(s) 203, 281, 346, 358
BceAI ACGGC 1 cut(s) 233
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BciT130I CCWGG 1 cut(s) 287
BfaI CTAG 1 cut(s) 544
BglI GCCNNNNNGGC 1 cut(s) 333
BisI GCNGC 9 cut(s) 24, 238, 241, 263, 328, 331, 334, 337, 503
BlsI GCNGC 9 cut(s) 25, 239, 242, 264, 329, 332, 335, 338, 504
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 3 cut(s) 70, 270, 540
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 1 cut(s) 287
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 66, 328
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 480, 510
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse3DI GCAATG 3 cut(s) 346, 484, 529
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 66, 328
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 3 cut(s) 346, 484, 529
BseMII CTCAG 1 cut(s) 368
BseRI GAGGAG 1 cut(s) 504
BseXI GCAGC 3 cut(s) 227, 249, 514
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 3 cut(s) 219, 303, 330
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 3 cut(s) 71, 290, 542
BsiEI CGRYCG 1 cut(s) 301
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 3 cut(s) 71, 290, 542
Bsp143I GATC 1 cut(s) 418
BspANI GGCC 3 cut(s) 71, 290, 542
BspCNI CTCAG 1 cut(s) 369
BspFNI CGCG 3 cut(s) 219, 303, 330
BspLI GGNNCC 1 cut(s) 72
BsrBI CCGCTC 1 cut(s) 258
BsrDI GCAATG 3 cut(s) 346, 484, 529
BssECI CCNNGG 2 cut(s) 66, 328
BssMI GATC 1 cut(s) 418
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 260, 305
BstDEI CTNAG 1 cut(s) 377
BstDSI CCRYGG 1 cut(s) 328
BstFNI CGCG 3 cut(s) 219, 303, 330
BstHHI GCGC 5 cut(s) 23, 42, 221, 309, 370
BstKTI GATC 1 cut(s) 421
BstMBI GATC 1 cut(s) 418
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 4 cut(s) 48, 333, 336, 367
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstUI CGCG 3 cut(s) 219, 303, 330
BstV1I GCAGC 3 cut(s) 227, 249, 514
BsuRI GGCC 3 cut(s) 71, 290, 542
BtgI CCRYGG 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 260, 305
CaiI CAGNNNCTG 1 cut(s) 376
CfoI GCGC 5 cut(s) 23, 42, 221, 309, 370
Cfr13I GGNCC 3 cut(s) 70, 270, 540
Cfr42I CCGCGG 1 cut(s) 331
CseI GACGC 1 cut(s) 118
Csp6I GTAC 1 cut(s) 415
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviAII CATG 1 cut(s) 187
CviJI RGCY 6 cut(s) 71, 209, 240, 290, 505, 542
CviKI_1 RGCY 6 cut(s) 71, 209, 240, 290, 505, 542
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 1 cut(s) 377
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 1 cut(s) 190
FaiI YATR 4 cut(s) 188, 347, 359, 536
FaqI GGGAC 1 cut(s) 395
FatI CATG 1 cut(s) 186
FauI CCCGC 2 cut(s) 190, 397
FblI GTMKAC 1 cut(s) 136
Fnu4HI GCNGC 9 cut(s) 24, 238, 241, 263, 328, 331, 334, 337, 503
Fsp4HI GCNGC 9 cut(s) 24, 238, 241, 263, 328, 331, 334, 337, 503
FspBI CTAG 1 cut(s) 544
GlaI GCGC 5 cut(s) 22, 41, 220, 308, 369
GluI GCNGC 9 cut(s) 24, 238, 241, 263, 328, 331, 334, 337, 503
HaeIII GGCC 3 cut(s) 71, 290, 542
HgaI GACGC 1 cut(s) 118
HhaI GCGC 5 cut(s) 23, 42, 221, 309, 370
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 1 cut(s) 190
Hin6I GCGC 5 cut(s) 21, 40, 219, 307, 368
HinP1I GCGC 5 cut(s) 21, 40, 219, 307, 368
HinfI GANTC 2 cut(s) 138, 373
HphI GGTGA 2 cut(s) 133, 145
Hpy166II GTNNAC 4 cut(s) 32, 137, 192, 415
Hpy188I TCNGA 2 cut(s) 176, 378
Hpy188III TCNNGA 2 cut(s) 458, 509
Hpy8I GTNNAC 4 cut(s) 32, 137, 192, 415
Hpy99I CGWCG 2 cut(s) 206, 254
HpyCH4III ACNGT 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 1 cut(s) 442
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 333, 336, 367
HpyF3I CTNAG 1 cut(s) 377
HpySE526I ACGT 1 cut(s) 450
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 1 cut(s) 190
HspAI GCGC 5 cut(s) 21, 40, 219, 307, 368
KspI CCGCGG 1 cut(s) 331
Kzo9I GATC 1 cut(s) 418
LpnPI CCDG 7 cut(s) 50, 66, 243, 253, 272, 299, 422
Lsp1109I GCAGC 3 cut(s) 227, 249, 514
MaeI CTAG 1 cut(s) 544
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 1 cut(s) 151
MalI GATC 1 cut(s) 420
MbiI CCGCTC 1 cut(s) 258
MboI GATC 1 cut(s) 418
MboII GAAGA 5 cut(s) 16, 190, 457, 479, 524
MluCI AATT 1 cut(s) 320
MlyI GAGTC 2 cut(s) 132, 382
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 8 cut(s) 49, 94, 170, 174, 182, 372, 482, 516
MseI TTAA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 3 cut(s) 219, 303, 330
MwoI GCNNNNNNNGC 4 cut(s) 48, 333, 336, 367
NdeII GATC 1 cut(s) 418
NlaIII CATG 1 cut(s) 190
NlaIV GGNNCC 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PkrI GCNGC 9 cut(s) 25, 239, 242, 264, 329, 332, 335, 338, 504
PleI GAGTC 2 cut(s) 132, 381
PpsI GAGTC 2 cut(s) 132, 381
Psp1406I AACGTT 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 3 cut(s) 70, 270, 540
PstNI CAGNNNCTG 1 cut(s) 376
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SacII CCGCGG 1 cut(s) 331
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 9 cut(s) 24, 238, 241, 263, 328, 331, 334, 337, 503
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 3 cut(s) 70, 270, 540
SchI GAGTC 2 cut(s) 132, 382
ScrFI CCNGG 1 cut(s) 287
SetI ASST 4 cut(s) 162, 174, 441, 453
Sfr303I CCGCGG 1 cut(s) 331
SgrBI CCGCGG 1 cut(s) 331
SinI GGWCC 1 cut(s) 270
Sse9I AATT 1 cut(s) 320
SspMI CTAG 1 cut(s) 544
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 123
TaiI ACGT 1 cut(s) 453
TaqI TCGA 3 cut(s) 204, 297, 318
TasI AATT 1 cut(s) 320
TauI GCSGC 6 cut(s) 26, 240, 330, 333, 336, 339
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 3 cut(s) 240, 262, 502
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 2 cut(s) 17, 422
VpaK11BI GGWCC 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 136
XspI CTAG 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.