Rroxscaffold_7G00158070

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008843
Physical Location & Seq
Reverse (-)
1653043 .. 1654068
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00158070.1

Sequence Viewer

Length: 564 bp
ATGAACAAGTACCAAAGTTGTGCCTCTTGCAGACACAACCGCTCAAAATGTGTATGGGATTGTCCACTGGCCCCGTACTTCCCTTCCCACATGCACCAAAAATTCAAGAATGCCCGCAAGGTGTTCGGCGAAAGTCACCTGAAGCAGTTGCTCCGGAGCCTACCCCCTCACAAAAGAAGCACTGCCATGAAGCACATTATAGCCGAAGCTGACATCCGAGTCGCCGACCCGGTTGGTGGAAGTTATGGACTTATAAGGAACTTGGAGCTGAAGATTGCGGAGGAAGAGGAGGAGCTCCGCCGCTTGCGCAATGTACTTAGTGTGTTTCGTGGCGGAGGTAGTGGTAGTGTTGTGGCACAGCCTTTGGTTCAGAAACAGGGATACATTGTTCAAGAACATGATAACATCAATTATCAGGGATATCATATTCCAGTATTCCCTCTTGTTCCTGGTGGATTTCTTCATGCACTCCTTCATCAAGAACAGCGCAACAACATGTTTGATTTAAGGGATGAAGTTGTGCTGGAACATGATGTCAACCCAAATCAGGGTGAGGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

187

Amino Acids

21.39

Weight (kDa)

7.78

Isoelectric Point (pI)

72.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 6 - 102 4.6e-29 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AasI GACNNNNNNGTC 1 cut(s) 218
Acc16I TGCGCA 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 42
AccIII TCCGGA 1 cut(s) 153
AciI CCGC 6 cut(s) 40, 115, 278, 298, 301, 333
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 2 cut(s) 161, 290
AfaI GTAC 3 cut(s) 11, 77, 315
AfiI CCNNNNNNNGG 3 cut(s) 236, 547, 548
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 2 cut(s) 106, 392
AjnI CCWGG 1 cut(s) 448
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 3 cut(s) 209, 268, 295
AluI AGCT 3 cut(s) 209, 268, 295
Alw21I GWGCWC 1 cut(s) 297
Aor13HI TCCGGA 1 cut(s) 153
AoxI GGCC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 101
AspLEI GCGC 2 cut(s) 309, 489
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 128, 563
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 450
BciVI GTATCC 1 cut(s) 374
BcnI CCSGG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 374
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
Bme1390I CCNGG 2 cut(s) 230, 450
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 72, 158
BmrFI CCNGG 2 cut(s) 230, 450
BpuMI CCSGG 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 153
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 547, 548
Bse1I ACTGG 2 cut(s) 72, 431
Bse3DI GCAATG 1 cut(s) 316
BseAI TCCGGA 1 cut(s) 153
BseBI CCWGG 1 cut(s) 450
BseGI GGATG 2 cut(s) 213, 517
BseLI CCNNNNNNNGG 3 cut(s) 236, 547, 548
BseMI GCAATG 1 cut(s) 316
BseNI ACTGG 2 cut(s) 72, 431
BseRI GAGGAG 2 cut(s) 302, 305
BshFI GGCC 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 2 cut(s) 154, 230
BslI CCNNNNNNNGG 3 cut(s) 236, 547, 548
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 153
BspACI CCGC 6 cut(s) 40, 115, 278, 298, 301, 333
BspANI GGCC 1 cut(s) 71
BspEI TCCGGA 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 72, 158
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 316
BsrI ACTGG 2 cut(s) 72, 431
Bst2UI CCWGG 1 cut(s) 450
Bst6I CTCTTC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 115, 305
BstDEI CTNAG 1 cut(s) 317
BstF5I GGATG 2 cut(s) 213, 517
BstHHI GCGC 2 cut(s) 309, 489
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 2 cut(s) 94, 499
BstSCI CCNGG 2 cut(s) 228, 448
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 71
BtsCI GGATG 2 cut(s) 213, 517
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 65, 180
Cac8I GCNNGC 2 cut(s) 115, 305
CfoI GCGC 2 cut(s) 309, 489
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 3 cut(s) 10, 76, 314
CviAII CATG 6 cut(s) 91, 187, 398, 464, 496, 530
CviJI RGCY 7 cut(s) 71, 159, 203, 209, 268, 295, 361
CviKI_1 RGCY 7 cut(s) 71, 159, 203, 209, 268, 295, 361
CviQI GTAC 3 cut(s) 10, 76, 314
DdeI CTNAG 1 cut(s) 317
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EciI GGCGGA 2 cut(s) 287, 348
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 161, 290
EcoICRI GAGCTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 448
EcoRV GATATC 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 6 cut(s) 94, 190, 401, 467, 499, 533
FatI CATG 6 cut(s) 90, 186, 397, 463, 495, 529
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 2 cut(s) 200, 524
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 301
FspI TGCGCA 1 cut(s) 308
GlaI GCGC 2 cut(s) 308, 488
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 1 cut(s) 71
HapII CCGG 2 cut(s) 154, 230
HhaI GCGC 2 cut(s) 309, 489
Hin1II CATG 6 cut(s) 94, 190, 401, 467, 499, 533
Hin6I GCGC 2 cut(s) 307, 487
HinP1I GCGC 2 cut(s) 307, 487
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 1 cut(s) 219
HpaII CCGG 2 cut(s) 154, 230
HphI GGTGA 2 cut(s) 128, 563
Hpy166II GTNNAC 2 cut(s) 65, 538
Hpy188I TCNGA 2 cut(s) 218, 372
Hpy188III TCNNGA 4 cut(s) 106, 154, 392, 479
Hpy8I GTNNAC 2 cut(s) 65, 538
HpyAV CCTTC 2 cut(s) 93, 482
HpyCH4V TGCA 3 cut(s) 30, 94, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 6 cut(s) 94, 190, 401, 467, 499, 533
HspAI GCGC 2 cut(s) 307, 487
Kpn2I TCCGGA 1 cut(s) 153
LmnI GCTCC 5 cut(s) 156, 156, 265, 292, 300
MaeIII GTNAC 1 cut(s) 134
MbiI CCGCTC 1 cut(s) 42
MboII GAAGA 3 cut(s) 283, 296, 452
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 2 cut(s) 101, 409
MlyI GAGTC 1 cut(s) 228
MnlI CCTC 9 cut(s) 34, 177, 274, 280, 283, 329, 450, 547, 550
MroI TCCGGA 1 cut(s) 153
MseI TTAA 1 cut(s) 506
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 2 cut(s) 154, 230
MspR9I CCNGG 2 cut(s) 230, 450
Mva1269I GAATGC 1 cut(s) 115
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 1 cut(s) 306
NciI CCSGG 1 cut(s) 230
NlaIII CATG 6 cut(s) 94, 190, 401, 467, 499, 533
NlaIV GGNNCC 2 cut(s) 72, 158
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 308
NspI RCATGY 2 cut(s) 94, 499
PciI ACATGT 1 cut(s) 495
PctI GAATGC 1 cut(s) 115
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PscI ACATGT 1 cut(s) 495
PsiI TTATAA 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 2 cut(s) 72, 158
PspPI GGNCC 1 cut(s) 70
RsaI GTAC 3 cut(s) 11, 77, 315
RsaNI GTAC 3 cut(s) 10, 76, 314
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 506
SatI GCNGC 1 cut(s) 301
Sau96I GGNCC 1 cut(s) 70
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 2 cut(s) 230, 450
SduI GDGCHC 1 cut(s) 297
SetI ASST 6 cut(s) 123, 141, 211, 270, 297, 340
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 2 cut(s) 101, 409
SsiI CCGC 6 cut(s) 40, 115, 278, 298, 301, 333
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 228, 448
TasI AATT 2 cut(s) 101, 409
TatI WGTACW 1 cut(s) 313
TauI GCSGC 1 cut(s) 303
Tru1I TTAA 1 cut(s) 506
Tru9I TTAA 1 cut(s) 506
TscAI CASTG 2 cut(s) 72, 187
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 5 cut(s) 17, 203, 452, 464, 528
TspRI CASTG 2 cut(s) 72, 187
XapI RAATTY 1 cut(s) 101
XceI RCATGY 2 cut(s) 94, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.