Rw6G043450

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008843
Physical Location & Seq
Forward (+)
66770291 .. 66770833
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043450.1

Sequence Viewer

Length: 543 bp
ATGAACAAGTACCAAAGTTGTGCCTCTTGCAGACACAACCGCTCAAAATGCGTACGGGATTGTCCACTGGCCCCGTACTTCCCTTCCCACATGCACCAAAAATTCAAGAATGCCCGCAAGGTGTTCGGCGAAAGTCACCTGAAGCAGTTGCTCCGGAGCCTACCTCCTCACAAAAGAAGCACTGCCATGAAGCACATTATAGCCGAAGCTGACATCCGAGTCGCCGATCCGGTTGGTGGAAGTTATGGACTTGTAAGGAAATTGGAGCTGAAGATTGCGGAGGAAGAGGAGGAGCTCCGCCGCTTGCGCAATGTACTTAGTGTGTTTCGTGGCAGAGGTAGTGGTAGTGTTGTGGCACAGCCTGTGGTTCAGAAACAGGGATACATTGTTCAAGAACATGATAACATCAATTATCAGGGATATCATATTCCAGTACTCCCTATTGTTCCTGGTGGATTTCTTCATGCACTCCTTCATCAAGAACAGCGCAACAACATGTTTGATTTAAGGTTTGTCCAAGTAACGATGGTAATAAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

180

Amino Acids

20.6

Weight (kDa)

9.79

Isoelectric Point (pI)

68.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 6 - 102 1.8e-29 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
Acc16I TGCGCA 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 42
AccIII TCCGGA 1 cut(s) 153
AciI CCGC 5 cut(s) 40, 115, 278, 298, 301
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 1 cut(s) 101
AcuI CTGAAG 2 cut(s) 161, 290
AfaI GTAC 5 cut(s) 11, 54, 77, 315, 435
AfiI CCNNNNNNNGG 1 cut(s) 236
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 2 cut(s) 106, 392
AjnI CCWGG 1 cut(s) 448
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 3 cut(s) 209, 268, 295
AluI AGCT 3 cut(s) 209, 268, 295
Alw21I GWGCWC 1 cut(s) 297
AlwI GGATC 1 cut(s) 221
Aor13HI TCCGGA 1 cut(s) 153
AoxI GGCC 1 cut(s) 69
ApoI RAATTY 1 cut(s) 101
AspLEI GCGC 2 cut(s) 309, 489
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 128
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 297
BccI CCATC 1 cut(s) 520
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 450
BciVI GTATCC 1 cut(s) 374
BfuI GTATCC 1 cut(s) 374
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
BmcAI AGTACT 1 cut(s) 435
Bme1390I CCNGG 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 72, 158
BmrFI CCNGG 1 cut(s) 450
BplI GAGNNNNNCTC 2 cut(s) 148, 180
BsaWI WCCGGW 2 cut(s) 153, 229
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 72, 431
Bse3DI GCAATG 1 cut(s) 316
BseAI TCCGGA 1 cut(s) 153
BseBI CCWGG 1 cut(s) 450
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 1 cut(s) 236
BseMI GCAATG 1 cut(s) 316
BseNI ACTGG 2 cut(s) 72, 431
BseRI GAGGAG 3 cut(s) 156, 302, 305
BshFI GGCC 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 2 cut(s) 154, 230
BsiWI CGTACG 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 236
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 153
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 5 cut(s) 40, 115, 278, 298, 301
BspANI GGCC 1 cut(s) 71
BspEI TCCGGA 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 72, 158
BspPI GGATC 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 316
BsrI ACTGG 2 cut(s) 72, 431
BssMI GATC 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 450
Bst6I CTCTTC 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 115, 305
BstDEI CTNAG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 213
BstHHI GCGC 2 cut(s) 309, 489
BstKTI GATC 1 cut(s) 229
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 48, 306
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 2 cut(s) 94, 499
BstSCI CCNGG 1 cut(s) 448
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 1 cut(s) 71
BtsCI GGATG 1 cut(s) 213
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 65, 180
Cac8I GCNNGC 2 cut(s) 115, 305
CfoI GCGC 2 cut(s) 309, 489
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 5 cut(s) 10, 53, 76, 314, 434
CviAII CATG 5 cut(s) 91, 187, 398, 464, 496
CviJI RGCY 7 cut(s) 71, 159, 203, 209, 268, 295, 361
CviKI_1 RGCY 7 cut(s) 71, 159, 203, 209, 268, 295, 361
CviQI GTAC 5 cut(s) 10, 53, 76, 314, 434
DdeI CTNAG 1 cut(s) 317
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 2 cut(s) 161, 290
EcoICRI GAGCTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 448
EcoRV GATATC 1 cut(s) 422
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 5 cut(s) 94, 190, 401, 467, 499
FaiI YATR 8 cut(s) 92, 188, 200, 246, 399, 426, 465, 497
FatI CATG 5 cut(s) 90, 186, 397, 463, 495
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 301
FspI TGCGCA 1 cut(s) 308
GlaI GCGC 2 cut(s) 308, 488
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 1 cut(s) 71
HapII CCGG 2 cut(s) 154, 230
HhaI GCGC 2 cut(s) 309, 489
Hin1II CATG 5 cut(s) 94, 190, 401, 467, 499
Hin6I GCGC 2 cut(s) 307, 487
HinP1I GCGC 2 cut(s) 307, 487
HinfI GANTC 1 cut(s) 219
HpaII CCGG 2 cut(s) 154, 230
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 65
Hpy188I TCNGA 2 cut(s) 218, 372
Hpy188III TCNNGA 4 cut(s) 106, 154, 392, 479
Hpy8I GTNNAC 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 93, 482
HpyCH4V TGCA 3 cut(s) 30, 94, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 306
HpyF3I CTNAG 1 cut(s) 317
Hsp92II CATG 5 cut(s) 94, 190, 401, 467, 499
HspAI GCGC 2 cut(s) 307, 487
Kpn2I TCCGGA 1 cut(s) 153
Kzo9I GATC 1 cut(s) 226
LmnI GCTCC 5 cut(s) 156, 156, 265, 292, 300
MaeIII GTNAC 2 cut(s) 134, 520
MalI GATC 1 cut(s) 228
MbiI CCGCTC 1 cut(s) 42
MboI GATC 1 cut(s) 226
MboII GAAGA 3 cut(s) 283, 296, 452
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 3 cut(s) 101, 260, 409
MlyI GAGTC 1 cut(s) 228
MnlI CCTC 7 cut(s) 34, 174, 177, 274, 280, 283, 329
MroI TCCGGA 1 cut(s) 153
MseI TTAA 1 cut(s) 506
MslI CAYNNNNRTG 1 cut(s) 185
MspI CCGG 2 cut(s) 154, 230
MspR9I CCNGG 1 cut(s) 450
Mva1269I GAATGC 1 cut(s) 115
MvaI CCWGG 1 cut(s) 450
MwoI GCNNNNNNNGC 2 cut(s) 48, 306
NdeII GATC 1 cut(s) 226
NlaIII CATG 5 cut(s) 94, 190, 401, 467, 499
NlaIV GGNNCC 2 cut(s) 72, 158
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 308
NspI RCATGY 2 cut(s) 94, 499
PciI ACATGT 1 cut(s) 495
PctI GAATGC 1 cut(s) 115
Pfl23II CGTACG 1 cut(s) 52
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PscI ACATGT 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspLI CGTACG 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 72, 158
PspPI GGNCC 1 cut(s) 70
RsaI GTAC 5 cut(s) 11, 54, 77, 315, 435
RsaNI GTAC 5 cut(s) 10, 53, 76, 314, 434
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 297
SaqAI TTAA 1 cut(s) 506
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 1 cut(s) 70
ScaI AGTACT 1 cut(s) 435
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 450
SduI GDGCHC 1 cut(s) 297
SetI ASST 8 cut(s) 123, 141, 166, 211, 270, 297, 340, 512
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 3 cut(s) 101, 260, 409
SsiI CCGC 5 cut(s) 40, 115, 278, 298, 301
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 448
TasI AATT 3 cut(s) 101, 260, 409
TatI WGTACW 2 cut(s) 313, 433
TauI GCSGC 1 cut(s) 303
Tru1I TTAA 1 cut(s) 506
Tru9I TTAA 1 cut(s) 506
TscAI CASTG 2 cut(s) 72, 187
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 17, 203, 452, 464
TspRI CASTG 2 cut(s) 72, 187
XapI RAATTY 1 cut(s) 101
XceI RCATGY 2 cut(s) 94, 499
ZrmI AGTACT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.