FvH4_2g32920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013623
Physical Location & Seq
Reverse (-)
24828516 .. 24831857
3342 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32920.t1

Sequence Viewer

Length: 1437 bp
ATGGCGACTCTGCCGGCGAATTACGTCACTCTCCTCCAGCTGAAGGAGCGGTACCTGAAGGAGAAGGAAGAGCAGGAACGGAGGGAGAAGGAGGAGGAGGAGCGAGCGCAGAAGGAGAAGGAAGAGGAGGAGGAGGAGCTCCGGCGGCAGAAGCTGAAAGAAGAGGAGGCGAAATTGCGGAAGCCAGACGGCGTCGTTGTGGTTAGGAATAGGGGGAGGTTTGATCGGAGGGAGGTTCGGCAGGTGGTTGATCGGAGGGAGTTTCGGCCGGTGGTTGATCGGAGAGAGTTTCCGCCGGTGGTTGATCGGAATGTTTCGGAGTGCGAGGGCGGGGAGATAGAGGGGAAGAGCGAGGAGCCGGAGGAGGAGAAGAAATGGAAGAAGAAGAAGAAGAAATGGGGGAAGAGGAGGAAGAATGTTAGGGCAGAGAAGGAGGAGGTGGGTAGTGAGAAGGAAGGTATAGCAGTTCCGGTGGTTATAGAGAGTGGCGAGAAGGCAAGTGATAGTGTGAGAAGAAATGGAGGAGAAAGGAGGAGAGCTGGTGGTGTTGAGTTTAGGGCAAGGAGCAAGAATGTGGAGGAGATTCAACCGAAATTTAGGAATTTGAAGATAGCTGGTGAGACTGAAATTTCGGGTGATACGTTGAGGAGGCCGAATAGCAGCAATGCAGGTGCGAAAGATGAGATTGAGCCGAAAGTTAGTGGCTTCGGCGTGAATGGTGAGACCGAGAGAAGGAATGGTAGGTGGAGGAGGCCAAATTACAGGAATGTGGGAGCCAAGGAAGAGATTGAACAGGAAGGTAAGGACTTCAGGATGAATGGCGAGACTGAAAGAGGAAATGATAGGTGGAGGAGGCCAAATCGCAGGAATGGGCGTTCTAGGGAGGAGATTGAGCCAAAAGTGAGGGACTTTGAGATGAATGGTGAGACTGAAAGAGGGAGTGATAGGCTGATGGGGGCAAATATGGAGGTGAGTGAGCCAAAAGTTAAGGATGTAAGGAAGGATGGTGAGACTGAAAGAGAGGATAGGCTAAAGGGGCCGAATAGTGAGGAGGAGAGTGAACCAAAAGTCAGAGAATTAACAATGACTGGTGAGATTGGAGGAGGGAGAGATAGACTGTGGAGGCCGAAGGGCAAGAATGTGGGTGGGAAGGATGAGACTGGGCCGAAAGTTATGGACCCATTGGTGAAGGCTGATAGTGAAAGAGGGGATGAAAGGTTCAGGAGGCCAAGTTGGTCTGATTACCGTGGTAATAGAAAACCTTGGGGTTACAAGAAAGAACAGTGGGTTGGTCTGGCTTGGGTTAAGAAGGGAGAGGTTTCTGATGGCAATGCTGCTGGAGTTCAAAGCTCGGAGCATTCTATCTCTGGTTCAACTAGAGCGTCTTTGAATGCAAATCCCCTGAAATCGGGCGGCCCTAGGAGAGTGATCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

479

Amino Acids

55.21

Weight (kDa)

9.47

Isoelectric Point (pI)

66.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32920
malus_domestica MD15G1067700.v1.1
prunus_persica Prupe.1G421600_v2.0.a1
pyrus_communis pycom08g06650 pycom15g06420
rosa_chinensis RchiOBHm_Chr6g0309801
rosa_laevigata RLG00000010535
rosa_multiflora Rmu_co8328543.1_g000001 Rmu_ssc0000373.1_g000006
rosa_roxburghii Rroxscaffold_7G00159170
rosa_rugosa Rorug06G0374700 Rorug06G0374800 Rorug06G0374900
rosa_samantha Rh6AG488200 Rh6BG497800 Rh6CG502600 Rh6DG488700
rosa_wichuraiana Rw6G042520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 232, 659
Acc36I ACCTGC 2 cut(s) 232, 659
Acc65I GGTACC 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 49
AciI CCGC 6 cut(s) 49, 145, 178, 293, 330, 1413
AclWI GGATC 1 cut(s) 1423
AcoI YGGCCR 1 cut(s) 266
AcsI RAATTY 3 cut(s) 593, 601, 627
AcuI CTGAAG 3 cut(s) 62, 77, 793
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 3 cut(s) 43, 732, 1408
AgsI TTSAA 6 cut(s) 587, 607, 791, 1346, 1374, 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 1272, 1304
AloI GAACNNNNNNTCC 4 cut(s) 859, 891, 1202, 1234
AluBI AGCT 6 cut(s) 40, 139, 154, 539, 614, 1350
AluI AGCT 6 cut(s) 40, 139, 154, 539, 614, 1350
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 6 cut(s) 614, 716, 818, 920, 1004, 1151
AlwI GGATC 1 cut(s) 1423
AlwNI CAGNNNCTG 1 cut(s) 154
AoxI GGCC 9 cut(s) 266, 650, 752, 854, 1037, 1124, 1163, 1226, 1414
ApeKI GCWGC 2 cut(s) 660, 1334
ApoI RAATTY 3 cut(s) 593, 601, 627
ArsI GACNNNNNNTTYG 2 cut(s) 613, 645
Asp718I GGTACC 1 cut(s) 51
AspA2I CCTAGG 1 cut(s) 1418
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 4 cut(s) 1037, 1163, 1177, 1415
AsuHPI GGTGA 8 cut(s) 629, 647, 731, 935, 982, 1019, 1103, 1198
AvaII GGWCC 1 cut(s) 1177
AvrII CCTAGG 1 cut(s) 1418
BanI GGYRCC 1 cut(s) 51
BanII GRGCYC 1 cut(s) 141
BarI GAAGNNNNNNTAC 2 cut(s) 35, 67
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 2 cut(s) 672, 1321
BccI CCATC 3 cut(s) 946, 998, 1319
BceAI ACGGC 1 cut(s) 205
BcoDI GTCTC 6 cut(s) 614, 716, 818, 920, 1004, 1151
BfaI CTAG 3 cut(s) 879, 1377, 1419
BfuAI ACCTGC 2 cut(s) 232, 659
BisI GCNGC 4 cut(s) 146, 661, 1335, 1414
BlnI CCTAGG 1 cut(s) 1418
BlsI GCNGC 4 cut(s) 147, 662, 1336, 1415
Bme18I GGWCC 1 cut(s) 1177
BmgT120I GGNCC 4 cut(s) 1037, 1163, 1177, 1415
BmiI GGNNCC 5 cut(s) 53, 357, 775, 1038, 1179
BmrI ACTGGG 1 cut(s) 1170
BmuI ACTGGG 1 cut(s) 1170
BpmI CTGGAG 2 cut(s) 20, 1359
BsaHI GRCGYC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 716
BsaJI CCNNGG 4 cut(s) 777, 1246, 1262, 1418
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 4 cut(s) 526, 556, 1216, 1246
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 732, 1408
Bse118I RCCGGY 3 cut(s) 13, 268, 295
Bse1I ACTGG 2 cut(s) 1093, 1165
Bse3DI GCAATG 2 cut(s) 670, 1336
BseDI CCNNGG 4 cut(s) 777, 1246, 1262, 1418
BseGI GGATG 5 cut(s) 819, 997, 1009, 1159, 1216
BseLI CCNNNNNNNGG 3 cut(s) 43, 732, 1408
BseMI GCAATG 2 cut(s) 670, 1336
BseNI ACTGG 2 cut(s) 1093, 1165
BseX3I CGGCCG 1 cut(s) 266
BseXI GCAGC 2 cut(s) 672, 1321
Bsh1285I CGRYCG 1 cut(s) 269
BshFI GGCC 9 cut(s) 268, 652, 754, 856, 1039, 1126, 1165, 1228, 1416
BshNI GGYRCC 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 6 cut(s) 14, 142, 269, 296, 359, 470
BslFI GGGAC 1 cut(s) 920
BslI CCNNNNNNNGG 3 cut(s) 43, 732, 1408
BsmAI GTCTC 6 cut(s) 614, 716, 818, 920, 1004, 1151
BsmFI GGGAC 1 cut(s) 920
BsmI GAATGC 2 cut(s) 1357, 1396
BsnI GGCC 9 cut(s) 268, 652, 754, 856, 1039, 1126, 1165, 1228, 1416
Bso31I GGTCTC 1 cut(s) 716
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 5 cut(s) 223, 250, 277, 304, 1428
BspACI CCGC 6 cut(s) 49, 145, 178, 293, 330, 1413
BspANI GGCC 9 cut(s) 268, 652, 754, 856, 1039, 1126, 1165, 1228, 1416
BspLI GGNNCC 5 cut(s) 53, 357, 775, 1038, 1179
BspMI ACCTGC 2 cut(s) 232, 659
BspPI GGATC 1 cut(s) 1423
BspQI GCTCTTC 2 cut(s) 63, 341
BspT107I GGYRCC 1 cut(s) 51
BspTNI GGTCTC 1 cut(s) 716
BsrBI CCGCTC 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 670, 1336
BsrFI RCCGGY 3 cut(s) 13, 268, 295
BsrI ACTGG 2 cut(s) 1093, 1165
BssAI RCCGGY 3 cut(s) 13, 268, 295
BssECI CCNNGG 4 cut(s) 777, 1246, 1262, 1418
BssMI GATC 5 cut(s) 223, 250, 277, 304, 1428
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 3 cut(s) 777, 1262, 1418
Bst4CI ACNGT 3 cut(s) 1119, 1247, 1284
Bst6I CTCTTC 6 cut(s) 63, 117, 156, 341, 398, 777
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 15, 105
BstDSI CCRYGG 1 cut(s) 1246
BstF5I GGATG 5 cut(s) 819, 997, 1009, 1159, 1216
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 5 cut(s) 226, 253, 280, 307, 1431
BstMAI GTCTC 6 cut(s) 614, 716, 818, 920, 1004, 1151
BstMBI GATC 5 cut(s) 223, 250, 277, 304, 1428
BstMCI CGRYCG 1 cut(s) 269
BstMWI GCNNNNNNNGC 4 cut(s) 46, 145, 151, 1036
BstV1I GCAGC 2 cut(s) 672, 1321
BstZI CGGCCG 1 cut(s) 266
BsuRI GGCC 9 cut(s) 268, 652, 754, 856, 1039, 1126, 1165, 1228, 1416
BtgI CCRYGG 1 cut(s) 1246
BtsCI GGATG 5 cut(s) 819, 997, 1009, 1159, 1216
BtsIMutI CAGTG 1 cut(s) 1289
BveI ACCTGC 2 cut(s) 232, 659
Cac8I GCNNGC 2 cut(s) 15, 105
CaiI CAGNNNCTG 1 cut(s) 154
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 3 cut(s) 13, 268, 295
Cfr13I GGNCC 4 cut(s) 1037, 1163, 1177, 1415
CseI GACGC 2 cut(s) 181, 1371
Csp6I GTAC 1 cut(s) 52
CviQI GTAC 1 cut(s) 52
DpnI GATC 5 cut(s) 225, 252, 279, 306, 1430
DpnII GATC 5 cut(s) 223, 250, 277, 304, 1428
EaeI YGGCCR 1 cut(s) 266
EagI CGGCCG 1 cut(s) 266
Eam1104I CTCTTC 6 cut(s) 63, 117, 156, 341, 398, 777
EarI CTCTTC 6 cut(s) 63, 117, 156, 341, 398, 777
EciI GGCGGA 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 139
EclXI CGGCCG 1 cut(s) 266
Eco130I CCWWGG 3 cut(s) 777, 1262, 1418
Eco24I GRGCYC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 716
Eco47I GGWCC 1 cut(s) 1177
Eco52I CGGCCG 1 cut(s) 266
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 3 cut(s) 62, 77, 793
EcoICRI GAGCTC 1 cut(s) 139
EcoT14I CCWWGG 3 cut(s) 777, 1262, 1418
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 3 cut(s) 777, 1262, 1418
FaiI YATR 4 cut(s) 461, 479, 965, 1175
FaqI GGGAC 1 cut(s) 920
FauI CCCGC 1 cut(s) 323
Fnu4HI GCNGC 4 cut(s) 146, 661, 1335, 1414
FokI GGATG 5 cut(s) 826, 1004, 1016, 1166, 1223
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 4 cut(s) 146, 661, 1335, 1414
FspBI CTAG 3 cut(s) 879, 1377, 1419
GlaI GCGC 1 cut(s) 108
GluI GCNGC 4 cut(s) 146, 661, 1335, 1414
GsuI CTGGAG 2 cut(s) 20, 1359
HaeIII GGCC 9 cut(s) 268, 652, 754, 856, 1039, 1126, 1165, 1228, 1416
HapII CCGG 6 cut(s) 14, 142, 269, 296, 359, 470
HgaI GACGC 2 cut(s) 181, 1371
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 192
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HinfI GANTC 2 cut(s) 7, 583
HpaII CCGG 6 cut(s) 14, 142, 269, 296, 359, 470
HphI GGTGA 8 cut(s) 629, 647, 731, 935, 982, 1019, 1103, 1198
Hpy166II GTNNAC 1 cut(s) 1061
Hpy188I TCNGA 9 cut(s) 228, 255, 282, 309, 319, 1073, 1240, 1324, 1354
Hpy188III TCNNGA 2 cut(s) 811, 1222
Hpy8I GTNNAC 1 cut(s) 1061
Hpy99I CGWCG 1 cut(s) 197
HpyCH4III ACNGT 3 cut(s) 1119, 1247, 1284
HpyCH4IV ACGT 2 cut(s) 24, 641
HpyCH4V TGCA 2 cut(s) 668, 1394
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 145, 151, 1036
HpySE526I ACGT 2 cut(s) 24, 641
Hsp92I GRCGYC 1 cut(s) 192
HspAI GCGC 1 cut(s) 107
KpnI GGTACC 1 cut(s) 55
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Kzo9I GATC 5 cut(s) 223, 250, 277, 304, 1428
LguI GCTCTTC 2 cut(s) 63, 341
LmnI GCTCC 8 cut(s) 46, 100, 136, 144, 355, 564, 773, 1354
Lsp1109I GCAGC 2 cut(s) 672, 1321
MaeI CTAG 3 cut(s) 879, 1377, 1419
MaeII ACGT 2 cut(s) 24, 641
MaeIII GTNAC 2 cut(s) 25, 1268
MalI GATC 5 cut(s) 225, 252, 279, 306, 1430
MbiI CCGCTC 1 cut(s) 49
MboI GATC 5 cut(s) 223, 250, 277, 304, 1428
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 7 cut(s) 19, 173, 593, 601, 627, 757, 1076
MroNI GCCGGC 1 cut(s) 13
MseI TTAA 3 cut(s) 987, 1079, 1305
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 6 cut(s) 14, 142, 269, 296, 359, 470
Mva1269I GAATGC 2 cut(s) 1357, 1396
MwoI GCNNNNNNNGC 4 cut(s) 46, 145, 151, 1036
NaeI GCCGGC 1 cut(s) 15
NdeII GATC 5 cut(s) 223, 250, 277, 304, 1428
NgoMIV GCCGGC 1 cut(s) 13
NlaIV GGNNCC 5 cut(s) 53, 357, 775, 1038, 1179
NmuCI GTSAC 1 cut(s) 25
PaqCI CACCTGC 2 cut(s) 232, 659
PciSI GCTCTTC 2 cut(s) 63, 341
PcsI WCGNNNNNNNCGW 1 cut(s) 638
PctI GAATGC 2 cut(s) 1357, 1396
PdiI GCCGGC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 583
PflFI GACNNNGTC 1 cut(s) 191
PkrI GCNGC 4 cut(s) 147, 662, 1336, 1415
Psp124BI GAGCTC 1 cut(s) 141
PspN4I GGNNCC 5 cut(s) 53, 357, 775, 1038, 1179
PspPI GGNCC 4 cut(s) 1037, 1163, 1177, 1415
PstNI CAGNNNCTG 1 cut(s) 154
PsyI GACNNNGTC 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 2 cut(s) 63, 341
SaqAI TTAA 3 cut(s) 987, 1079, 1305
SatI GCNGC 4 cut(s) 146, 661, 1335, 1414
Sau3AI GATC 5 cut(s) 223, 250, 277, 304, 1428
Sau96I GGNCC 4 cut(s) 1037, 1163, 1177, 1415
SduI GDGCHC 1 cut(s) 141
SgrAI CRCCGGYG 1 cut(s) 295
SinI GGWCC 1 cut(s) 1177
Sse9I AATT 7 cut(s) 19, 173, 593, 601, 627, 757, 1076
SsiI CCGC 6 cut(s) 49, 145, 178, 293, 330, 1413
SspMI CTAG 3 cut(s) 879, 1377, 1419
SstI GAGCTC 1 cut(s) 141
StyI CCWWGG 3 cut(s) 777, 1262, 1418
TaaI ACNGT 3 cut(s) 1119, 1247, 1284
TaiI ACGT 2 cut(s) 27, 644
TaqII GACCGA 1 cut(s) 740
TasI AATT 7 cut(s) 19, 173, 593, 601, 627, 757, 1076
TauI GCSGC 2 cut(s) 148, 1416
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 3 cut(s) 987, 1079, 1305
Tru9I TTAA 3 cut(s) 987, 1079, 1305
TscAI CASTG 1 cut(s) 1289
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 2 cut(s) 660, 1334
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 3 cut(s) 830, 932, 1227
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 1 cut(s) 1289
Tth111I GACNNNGTC 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 1177
XapI RAATTY 3 cut(s) 593, 601, 627
XmaJI CCTAGG 1 cut(s) 1418
XspI CTAG 3 cut(s) 879, 1377, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.