MD15G1067700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013623
Physical Location & Seq
Reverse (-)
4703421 .. 4706562
3142 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1067700.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGGAGGTCACCAAATTCAAGAGGCCCCAATCTTTGCCCGCCAACTACGTCACCATCAACGATCTCAAGGAGCGCTGGCTCAGAGACCAGGAACGAAAACAGAAGGAGGAAGAACGACGGCAAGAGGAGAAGAAACGACAGGAAGAGGAGGAAAAACGACGACAAAAGGAGGAGGAACAACGAGAAGAAAAGCTCAGGCAGCGGAAGCTCCGAGAAGAGGAGGAAAAACGACGACAAGAGGAGAAGGAACGGCGAGGAGAAGAGCTCCGGCAGCGGAAGCTCCGAGAAGAGGAGGAAAAACGACGACAAGAGGAGAAGGACCGGCGAGAAGAAGAGCTCCGGCAGCGGAAGCTCCGAGAAGAGGAGGCGAATCAGAAGAAACAAAAGGCCGTCGTTTGGAAACCCGAAACCGGTTCCAAGCCTAGGTATGGTCGTAATCGGGGACAGTGTCGGCCGGTTAATCGGAGCGTTTCGGGGCGCGAGGGGGCTGAGCTGGAGGGGAAAAAAAGCGAGGAAGAAGAGCCCAAGGATAACGCCATCGTTAAGAGAACAGAAGCGGATGACAAGCCGAAGAATCGCGGTAATCGGCTAGAGTTTCGGCCGGTTAATCGGAGTGTTTCGGAATGTGAGGGTGCTGAGCTGGAGGGAAAAAGAAGCGAGGAAGGAGAACCGAAGGAAAGCGGCGTCGTTTGGAGAACCGGAACCGGTAATAATCCGAGAAATGAGACACAGTTTCGGACGGTGAATCGAAGTGGCCGGGCACCCTTGTTGGAGGGGAAAAGAAGCGTGGAAGAAGAGGCGAAGCAAAATGCCGTAGTCCAAAGAACCCAAACCGCTAACAAGCCGAGGAACCGAAGTGATCGGGGACAGTTTCGGCCGGTTAATAGTGGTGGTCGGGCACCCTCGCTGGAGGGGAAAAGAAGCGAGAAAGAAGAGCTTAAGCAAAACGCTGTTGTTCAGAGAACCGAAGCCGCTAACAAGCCGGGGAACCCAAGTGATCGGGGACAGCTTCGGCCTGTTAATCGGGGTGATCGGGTACCCTCGCTGGAGGGGAAAAGAAGCGAGGAAGAAGAGCCGAAGCAAAATGCCGTCGTTCAGGGAACCAAAATCGATAACAAGCCGAGGGATCGAAGTGAACGGGGACAGTTTCGGCCTGTTAATCGGGGCAATCGGGGCAATCGGGCACCCTCGCTGGATGGGAAAAGAAGCGAGCAAGAAGAGCTGAAGCAAAACGCCCTCGTTCAGAGAACCAAAATCGATAACAAGCGTCATCGGGCACAGTTTCGGCCAGTTAATCGTGGTGTCTCGGAGCGTGAGGGTGCTGAGATGGAGGGGAATGGAAGCGAGGAAGAACAGCCGAAGCAAAACGGCGTCGTTCAGAGAACCGAAACCGATATCAAGCCGAGGAATCGGAGAGATCGGGGGCAGTTTCGGCCGGTTAATCGGGGGGTTTCGGAGCTCGAGGTTGCTGAGCTGGAGGGGAAGAAAAGCGAGCGAGCAACCAGAGGAGAAGAAGAAATGGACGAAGAAGAAGAAGCAGAAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

516

Amino Acids

60.38

Weight (kDa)

9.62

Isoelectric Point (pI)

81.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013623)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32920
malus_domestica MD15G1067700.v1.1
prunus_persica Prupe.1G421600_v2.0.a1
pyrus_communis pycom08g06650 pycom15g06420
rosa_chinensis RchiOBHm_Chr6g0309801
rosa_laevigata RLG00000010535
rosa_multiflora Rmu_co8328543.1_g000001 Rmu_ssc0000373.1_g000006
rosa_roxburghii Rroxscaffold_7G00159170
rosa_rugosa Rorug06G0374700 Rorug06G0374800 Rorug06G0374900
rosa_samantha Rh6AG488200 Rh6BG497800 Rh6CG502600 Rh6DG488700
rosa_wichuraiana Rw6G042520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1036
AccB1I GGYRCC 4 cut(s) 760, 898, 1036, 1183
AccII CGCG 2 cut(s) 480, 579
AciI CCGC 9 cut(s) 39, 202, 274, 346, 557, 579, 681, 834, 972
AclWI GGATC 1 cut(s) 1134
AcoI YGGCCR 6 cut(s) 452, 599, 754, 875, 1286, 1433
AcsI RAATTY 1 cut(s) 14
AcuI CTGAAG 1 cut(s) 1244
AcyI GRCGYC 2 cut(s) 684, 1371
AfaI GTAC 1 cut(s) 1038
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 7 cut(s) 217, 289, 361, 396, 410, 423, 428
AflII CTTAAG 1 cut(s) 938
AgeI ACCGGT 2 cut(s) 410, 704
AgsI TTSAA 1 cut(s) 19
AjnI CCWGG 1 cut(s) 87
Alw21I GWGCWC 3 cut(s) 267, 339, 1461
Alw26I GTCTC 3 cut(s) 78, 719, 1309
AlwI GGATC 1 cut(s) 1134
Ama87I CYCGRG 1 cut(s) 1460
Aor51HI AGCGCT 1 cut(s) 74
ApeKI GCWGC 3 cut(s) 199, 271, 343
ApoI RAATTY 1 cut(s) 14
AsiGI ACCGGT 2 cut(s) 410, 704
Asp718I GGTACC 1 cut(s) 1036
AspA2I CCTAGG 1 cut(s) 422
AspLEI GCGC 2 cut(s) 75, 480
AspS9I GGNCC 2 cut(s) 24, 319
AsuC2I CCSGG 2 cut(s) 758, 984
AsuHPI GGTGA 3 cut(s) 43, 754, 1040
AvaI CYCGRG 1 cut(s) 1460
AvaII GGWCC 1 cut(s) 319
AvrII CCTAGG 1 cut(s) 422
BaeGI GKGCMC 4 cut(s) 763, 901, 1186, 1279
BanI GGYRCC 4 cut(s) 760, 898, 1036, 1183
BanII GRGCYC 4 cut(s) 267, 339, 525, 1461
Bbv12I GWGCWC 3 cut(s) 267, 339, 1461
BbvI GCAGC 3 cut(s) 211, 283, 355
BccI CCATC 4 cut(s) 62, 545, 1190, 1321
BceAI ACGGC 6 cut(s) 134, 266, 374, 797, 1073, 1384
BciT130I CCWGG 1 cut(s) 89
BcnI CCSGG 2 cut(s) 758, 984
BcoDI GTCTC 3 cut(s) 78, 719, 1309
BfaI CTAG 2 cut(s) 423, 590
BfoI RGCGCY 1 cut(s) 76
BfrI CTTAAG 1 cut(s) 938
BisI GCNGC 5 cut(s) 200, 272, 344, 682, 972
BlnI CCTAGG 1 cut(s) 422
BlpI GCTNAGC 3 cut(s) 489, 636, 1470
BlsI GCNGC 5 cut(s) 201, 273, 345, 683, 973
Bme1390I CCNGG 3 cut(s) 89, 758, 984
Bme18I GGWCC 1 cut(s) 319
BmeT110I CYCGRG 1 cut(s) 1460
BmgT120I GGNCC 2 cut(s) 24, 319
BmrFI CCNGG 3 cut(s) 89, 758, 984
BplI GAGNNNNNCTC 2 cut(s) 249, 281
BpmI CTGGAG 5 cut(s) 515, 662, 929, 1067, 1496
Bpu10I CCTNAGC 1 cut(s) 194
Bpu1102I GCTNAGC 3 cut(s) 489, 636, 1470
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 2 cut(s) 758, 984
Bsa29I ATCGAT 2 cut(s) 1110, 1257
BsaHI GRCGYC 2 cut(s) 684, 1371
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 6 cut(s) 422, 525, 845, 983, 1121, 1403
BsaWI WCCGGW 3 cut(s) 410, 698, 704
Bsc4I CCNNNNNNNGG 7 cut(s) 217, 289, 361, 396, 410, 423, 428
Bse118I RCCGGY 7 cut(s) 321, 410, 454, 601, 704, 877, 1435
Bse1I ACTGG 1 cut(s) 1289
BseBI CCWGG 1 cut(s) 89
BseCI ATCGAT 2 cut(s) 1110, 1257
BseDI CCNNGG 6 cut(s) 422, 525, 845, 983, 1121, 1403
BseGI GGATG 2 cut(s) 565, 1201
BseLI CCNNNNNNNGG 7 cut(s) 217, 289, 361, 396, 410, 423, 428
BseMII CTCAG 6 cut(s) 94, 208, 480, 627, 1314, 1461
BseNI ACTGG 1 cut(s) 1289
BseSI GKGCMC 4 cut(s) 763, 901, 1186, 1279
BseX3I CGGCCG 4 cut(s) 452, 599, 875, 1433
BseXI GCAGC 3 cut(s) 211, 283, 355
Bsh1236I CGCG 2 cut(s) 480, 579
Bsh1285I CGRYCG 4 cut(s) 455, 602, 878, 1436
BshNI GGYRCC 4 cut(s) 760, 898, 1036, 1183
BshTI ACCGGT 2 cut(s) 410, 704
BshVI ATCGAT 2 cut(s) 1110, 1257
BsiEI CGRYCG 4 cut(s) 455, 602, 878, 1436
BsiHKAI GWGCWC 3 cut(s) 267, 339, 1461
BsiHKCI CYCGRG 1 cut(s) 1460
BslFI GGGAC 4 cut(s) 456, 879, 1017, 1155
BslI CCNNNNNNNGG 7 cut(s) 217, 289, 361, 396, 410, 423, 428
BsmAI GTCTC 3 cut(s) 78, 719, 1309
BsmFI GGGAC 4 cut(s) 456, 879, 1017, 1155
Bso31I GGTCTC 1 cut(s) 78
BsoBI CYCGRG 1 cut(s) 1460
Bsp1286I GDGCHC 8 cut(s) 267, 339, 525, 763, 901, 1186, 1279, 1461
Bsp143I GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
Bsp1720I GCTNAGC 3 cut(s) 489, 636, 1470
BspACI CCGC 9 cut(s) 39, 202, 274, 346, 557, 579, 681, 834, 972
BspCNI CTCAG 6 cut(s) 93, 207, 481, 628, 1315, 1462
BspDI ATCGAT 2 cut(s) 1110, 1257
BspFNI CGCG 2 cut(s) 480, 579
BspPI GGATC 1 cut(s) 1134
BspQI GCTCTTC 6 cut(s) 255, 327, 513, 927, 1065, 1212
BspT107I GGYRCC 4 cut(s) 760, 898, 1036, 1183
BspTI CTTAAG 1 cut(s) 938
BspTNI GGTCTC 1 cut(s) 78
BsrFI RCCGGY 7 cut(s) 321, 410, 454, 601, 704, 877, 1435
BsrI ACTGG 1 cut(s) 1289
BssAI RCCGGY 7 cut(s) 321, 410, 454, 601, 704, 877, 1435
BssECI CCNNGG 6 cut(s) 422, 525, 845, 983, 1121, 1403
BssMI GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
BssNI GRCGYC 2 cut(s) 684, 1371
BssT1I CCWWGG 2 cut(s) 422, 525
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 6 cut(s) 447, 732, 742, 870, 1146, 1281
BstACI GRCGYC 2 cut(s) 684, 1371
BstAFI CTTAAG 1 cut(s) 938
BstC8I GCNNGC 5 cut(s) 39, 77, 1211, 1493, 1497
BstDEI CTNAG 6 cut(s) 80, 194, 489, 636, 1323, 1470
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 2 cut(s) 565, 1201
BstFNI CGCG 2 cut(s) 480, 579
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 75, 480
BstKTI GATC 6 cut(s) 64, 862, 1000, 1033, 1129, 1420
BstMAI GTCTC 3 cut(s) 78, 719, 1309
BstMBI GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
BstMCI CGRYCG 4 cut(s) 455, 602, 878, 1436
BstMWI GCNNNNNNNGC 9 cut(s) 199, 205, 271, 277, 343, 349, 1173, 1219, 1432
BstNI CCWGG 1 cut(s) 89
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 3 cut(s) 87, 756, 982
BstSLI GKGCMC 4 cut(s) 763, 901, 1186, 1279
BstUI CGCG 2 cut(s) 480, 579
BstV1I GCAGC 3 cut(s) 211, 283, 355
BstZI CGGCCG 4 cut(s) 452, 599, 875, 1433
Bsu15I ATCGAT 2 cut(s) 1110, 1257
BsuTUI ATCGAT 2 cut(s) 1110, 1257
BtsCI GGATG 2 cut(s) 565, 1201
BtsIMutI CAGTG 1 cut(s) 452
Cac8I GCNNGC 5 cut(s) 39, 77, 1211, 1493, 1497
CfoI GCGC 2 cut(s) 75, 480
Cfr10I RCCGGY 7 cut(s) 321, 410, 454, 601, 704, 877, 1435
Cfr13I GGNCC 2 cut(s) 24, 319
ClaI ATCGAT 2 cut(s) 1110, 1257
CseI GACGC 3 cut(s) 673, 1256, 1360
Csp6I GTAC 1 cut(s) 1037
CspAI ACCGGT 2 cut(s) 410, 704
CviQI GTAC 1 cut(s) 1037
DdeI CTNAG 6 cut(s) 80, 194, 489, 636, 1323, 1470
DpnI GATC 6 cut(s) 63, 861, 999, 1032, 1128, 1419
DpnII GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
EaeI YGGCCR 6 cut(s) 452, 599, 754, 875, 1286, 1433
EagI CGGCCG 4 cut(s) 452, 599, 875, 1433
Ecl136II GAGCTC 3 cut(s) 265, 337, 1459
EclXI CGGCCG 4 cut(s) 452, 599, 875, 1433
Eco130I CCWWGG 2 cut(s) 422, 525
Eco24I GRGCYC 4 cut(s) 267, 339, 525, 1461
Eco31I GGTCTC 1 cut(s) 78
Eco32I GATATC 1 cut(s) 1396
Eco47I GGWCC 1 cut(s) 319
Eco47III AGCGCT 1 cut(s) 74
Eco52I CGGCCG 4 cut(s) 452, 599, 875, 1433
Eco53kI GAGCTC 3 cut(s) 265, 337, 1459
Eco57I CTGAAG 1 cut(s) 1244
Eco88I CYCGRG 1 cut(s) 1460
Eco91I GGTNACC 1 cut(s) 7
EcoICRI GAGCTC 3 cut(s) 265, 337, 1459
EcoO109I RGGNCCY 1 cut(s) 24
EcoO65I GGTNACC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 87
EcoRV GATATC 1 cut(s) 1396
EcoT14I CCWWGG 2 cut(s) 422, 525
EcoT38I GRGCYC 4 cut(s) 267, 339, 525, 1461
ErhI CCWWGG 2 cut(s) 422, 525
FaiI YATR 1 cut(s) 429
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 4 cut(s) 456, 879, 1017, 1155
FauI CCCGC 1 cut(s) 46
Fnu4HI GCNGC 5 cut(s) 200, 272, 344, 682, 972
FokI GGATG 2 cut(s) 572, 1208
FriOI GRGCYC 4 cut(s) 267, 339, 525, 1461
Fsp4HI GCNGC 5 cut(s) 200, 272, 344, 682, 972
FspBI CTAG 2 cut(s) 423, 590
GlaI GCGC 2 cut(s) 74, 479
GluI GCNGC 5 cut(s) 200, 272, 344, 682, 972
GsuI CTGGAG 5 cut(s) 515, 662, 929, 1067, 1496
HaeII RGCGCY 1 cut(s) 76
HgaI GACGC 3 cut(s) 673, 1256, 1360
HhaI GCGC 2 cut(s) 75, 480
Hin1I GRCGYC 2 cut(s) 684, 1371
Hin6I GCGC 2 cut(s) 73, 478
HinP1I GCGC 2 cut(s) 73, 478
HinfI GANTC 4 cut(s) 370, 574, 745, 1408
HphI GGTGA 3 cut(s) 43, 754, 1040
Hpy166II GTNNAC 1 cut(s) 1136
Hpy188III TCNNGA 1 cut(s) 19
Hpy8I GTNNAC 1 cut(s) 1136
Hpy99I CGWCG 8 cut(s) 120, 162, 234, 306, 395, 689, 1094, 1376
HpyAV CCTTC 6 cut(s) 97, 238, 310, 656, 667, 1535
HpyCH4III ACNGT 6 cut(s) 447, 732, 742, 870, 1146, 1281
HpyCH4IV ACGT 1 cut(s) 48
HpyF10VI GCNNNNNNNGC 9 cut(s) 199, 205, 271, 277, 343, 349, 1173, 1219, 1432
HpyF3I CTNAG 6 cut(s) 80, 194, 489, 636, 1323, 1470
HpySE526I ACGT 1 cut(s) 48
Hsp92I GRCGYC 2 cut(s) 684, 1371
HspAI GCGC 2 cut(s) 73, 478
KpnI GGTACC 1 cut(s) 1040
Kzo9I GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
LguI GCTCTTC 6 cut(s) 255, 327, 513, 927, 1065, 1212
LmnI GCTCC 9 cut(s) 70, 213, 270, 285, 342, 357, 465, 1309, 1456
Lsp1109I GCAGC 3 cut(s) 211, 283, 355
MaeI CTAG 2 cut(s) 423, 590
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 2 cut(s) 7, 49
MalI GATC 6 cut(s) 63, 861, 999, 1032, 1128, 1419
MboI GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
MhlI GDGCHC 8 cut(s) 267, 339, 525, 763, 901, 1186, 1279, 1461
MluCI AATT 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 750
MseI TTAA 9 cut(s) 459, 543, 606, 882, 939, 1020, 1158, 1293, 1440
MspA1I CMGCKG 3 cut(s) 202, 274, 346
MspCI CTTAAG 1 cut(s) 938
MspR9I CCNGG 3 cut(s) 89, 758, 984
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 2 cut(s) 480, 579
MwoI GCNNNNNNNGC 9 cut(s) 199, 205, 271, 277, 343, 349, 1173, 1219, 1432
NciI CCSGG 2 cut(s) 758, 984
NdeII GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
NmeAIII GCCGAG 3 cut(s) 870, 1146, 1428
NmuCI GTSAC 2 cut(s) 7, 49
PaeR7I CTCGAG 1 cut(s) 1460
PciSI GCTCTTC 6 cut(s) 255, 327, 513, 927, 1065, 1212
PfeI GAWTC 4 cut(s) 370, 574, 745, 1408
PflFI GACNNNGTC 1 cut(s) 447
PinAI ACCGGT 2 cut(s) 410, 704
PkrI GCNGC 5 cut(s) 201, 273, 345, 683, 973
Psp124BI GAGCTC 3 cut(s) 267, 339, 1461
Psp6I CCWGG 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 87
PspPI GGNCC 2 cut(s) 24, 319
PspXI VCTCGAGB 1 cut(s) 1460
PsyI GACNNNGTC 1 cut(s) 447
RsaI GTAC 1 cut(s) 1038
RsaNI GTAC 1 cut(s) 1037
SacI GAGCTC 3 cut(s) 267, 339, 1461
SapI GCTCTTC 6 cut(s) 255, 327, 513, 927, 1065, 1212
SaqAI TTAA 9 cut(s) 459, 543, 606, 882, 939, 1020, 1158, 1293, 1440
SatI GCNGC 5 cut(s) 200, 272, 344, 682, 972
Sau3AI GATC 6 cut(s) 61, 859, 997, 1030, 1126, 1417
Sau96I GGNCC 2 cut(s) 24, 319
ScrFI CCNGG 3 cut(s) 89, 758, 984
SduI GDGCHC 8 cut(s) 267, 339, 525, 763, 901, 1186, 1279, 1461
Sfr274I CTCGAG 1 cut(s) 1460
SinI GGWCC 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 1460
SmlI CTYRAG 3 cut(s) 65, 938, 1460
SmoI CTYRAG 3 cut(s) 65, 938, 1460
Sse9I AATT 1 cut(s) 14
SsiI CCGC 9 cut(s) 39, 202, 274, 346, 557, 579, 681, 834, 972
SspMI CTAG 2 cut(s) 423, 590
SstI GAGCTC 3 cut(s) 267, 339, 1461
StyD4I CCNGG 3 cut(s) 87, 756, 982
StyI CCWWGG 2 cut(s) 422, 525
TaaI ACNGT 6 cut(s) 447, 732, 742, 870, 1146, 1281
TaiI ACGT 1 cut(s) 51
TaqI TCGA 5 cut(s) 748, 1110, 1129, 1257, 1461
TasI AATT 1 cut(s) 14
TauI GCSGC 2 cut(s) 684, 974
TfiI GAWTC 4 cut(s) 370, 574, 745, 1408
Tru1I TTAA 9 cut(s) 459, 543, 606, 882, 939, 1020, 1158, 1293, 1440
Tru9I TTAA 9 cut(s) 459, 543, 606, 882, 939, 1020, 1158, 1293, 1440
TscAI CASTG 1 cut(s) 452
TseFI GTSAC 2 cut(s) 7, 49
TseI GCWGC 3 cut(s) 199, 271, 343
Tsp45I GTSAC 2 cut(s) 7, 49
TspRI CASTG 1 cut(s) 452
Tth111I GACNNNGTC 1 cut(s) 447
Vha464I CTTAAG 1 cut(s) 938
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 1 cut(s) 14
XhoI CTCGAG 1 cut(s) 1460
XmaJI CCTAGG 1 cut(s) 422
XspI CTAG 2 cut(s) 423, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.