FvH4_2g34080
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008124
Physical Location & Seq
Forward (+)
25409339 .. 25412358
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34080.t3

Sequence Viewer

Length: 849 bp
ATGGGCGATGCCAAAGCGACGTCGTTTTACCTCCCACCCGGTTGCCGATTCTACCCCTCCGAGGAGCAGCTCCTCTGCTACTACCTCTCCCGCAAGAACACCGCCGCCTCCGACGAGTCCGACCCCGGCGACAACGGCTACGATTTGATTAAAGAGCTCGACTTGTACGACCGCGACCCGTTTGATTTGCCGGACTATGCTTGCTACTCTTACGGCCGCGGCGGCCGGAGGCGGCACTGGTTCTGCTACACGGTTAGGGTTTTGAAGGAGCGGCGGGCGAGGAGCGGGTATTGGAAGAGGAAGGGAAGGGTTAGGGACGTCGTCGGCGGCGGCGGCGGCGGAGGGAAGGCGGTGCTTGGCCGGAGGAGTAGCTTTGTGTTTTATTTGGGGAATTCGCCCAAGACTGCTGTGCGGACTGACTGGGTGCTCTATCAGTACGCTCAGGTTGATCATCTTCAGGCCTCTTTTGTCCTCTGCCGAGTATTTGTGAGATCTCAGGGTGGAACTAACATATCAGAGAATGGGTTAAGTTCATGTGCTGAAGAAACTGTTTCCACAGTACGTCACATTGGAATTCAGCATGATGGATTTTATACTCCTCATATTGTTCCTGAGATTGACGGTGACAAATCTGTGCCTAGGAACACTGACAAGTCAAAAGATCAAAAGAGACCGGAGACTGAGGTAGACAAGCAAGTTGAGACTCGGCTGGTATCAAGTATTCAGACTAATGGACAGGTGCCACTATTAAGTGTTAGCAATCCAGTATTGCTTGACGGTTTGTCCTCTGAGCATCTACTATCCCTTATAGAGGGAGACTTCATAGAGTTGGATGATCTCATCGACTAA

Protein Analysis

283

Amino Acids

31.65

Weight (kDa)

6.11

Isoelectric Point (pI)

59.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 12 - 147 4.2e-22 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 23, 321
AccB1I GGYRCC 1 cut(s) 739
AccBSI CCGCTC 2 cut(s) 271, 285
AccI GTMKAC 1 cut(s) 687
AccII CGCG 2 cut(s) 174, 219
AcoI YGGCCR 3 cut(s) 214, 223, 358
AcsI RAATTY 2 cut(s) 391, 573
AcuI CTGAAG 2 cut(s) 440, 561
AcyI GRCGYC 2 cut(s) 20, 318
AfaI GTAC 3 cut(s) 167, 437, 561
AfiI CCNNNNNNNGG 2 cut(s) 61, 811
AgsI TTSAA 1 cut(s) 265
AhdI GACNNNNNGTC 1 cut(s) 781
AluBI AGCT 3 cut(s) 70, 157, 372
AluI AGCT 3 cut(s) 70, 157, 372
Alw21I GWGCWC 2 cut(s) 159, 429
Alw26I GTCTC 4 cut(s) 664, 671, 695, 810
AoxI GGCC 4 cut(s) 214, 223, 358, 459
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 2 cut(s) 391, 573
AspA2I CCTAGG 1 cut(s) 638
AsuC2I CCSGG 2 cut(s) 39, 126
AsuHPI GGTGA 1 cut(s) 635
AvrII CCTAGG 1 cut(s) 638
BanI GGYRCC 1 cut(s) 739
BanII GRGCYC 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 159, 429
BbvI GCAGC 1 cut(s) 79
BccI CCATC 1 cut(s) 578
BceAI ACGGC 2 cut(s) 151, 229
BclI TGATCA 1 cut(s) 448
BcnI CCSGG 2 cut(s) 39, 126
BcoDI GTCTC 4 cut(s) 664, 671, 695, 810
BfaI CTAG 1 cut(s) 639
BglI GCCNNNNNGGC 1 cut(s) 222
BglII AGATCT 1 cut(s) 491
BlnI CCTAGG 1 cut(s) 638
Bme1390I CCNGG 2 cut(s) 39, 126
BmeRI GACNNNNNGTC 1 cut(s) 781
BmiI GGNNCC 1 cut(s) 741
BmrFI CCNGG 2 cut(s) 39, 126
BmrI ACTGGG 1 cut(s) 430
BmsI GCATC 1 cut(s) 802
BmuI ACTGGG 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 441
BpuMI CCSGG 2 cut(s) 39, 126
BsaHI GRCGYC 2 cut(s) 20, 318
BsaI GGTCTC 1 cut(s) 664
BsaJI CCNNGG 4 cut(s) 60, 124, 217, 638
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 811
Bse1I ACTGG 3 cut(s) 242, 425, 764
BseDI CCNNGG 4 cut(s) 60, 124, 217, 638
BseGI GGATG 1 cut(s) 838
BseLI CCNNNNNNNGG 2 cut(s) 61, 811
BseMII CTCAG 5 cut(s) 455, 509, 603, 672, 780
BseNI ACTGG 3 cut(s) 242, 425, 764
BseRI GAGGAG 5 cut(s) 62, 77, 295, 379, 588
BseX3I CGGCCG 2 cut(s) 214, 223
BseXI GCAGC 1 cut(s) 79
Bsh1236I CGCG 2 cut(s) 174, 219
Bsh1285I CGRYCG 3 cut(s) 172, 217, 226
BshFI GGCC 4 cut(s) 216, 225, 360, 461
BshNI GGYRCC 1 cut(s) 739
BsiEI CGRYCG 3 cut(s) 172, 217, 226
BsiHKAI GWGCWC 2 cut(s) 159, 429
BsiSI CCGG 6 cut(s) 39, 126, 191, 226, 361, 674
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 2 cut(s) 61, 811
BsmAI GTCTC 4 cut(s) 664, 671, 695, 810
BsmFI GGGAC 1 cut(s) 329
BsnI GGCC 4 cut(s) 216, 225, 360, 461
Bso31I GGTCTC 1 cut(s) 664
Bsp1286I GDGCHC 2 cut(s) 159, 429
Bsp143I GATC 4 cut(s) 448, 491, 661, 835
BspANI GGCC 4 cut(s) 216, 225, 360, 461
BspCNI CTCAG 5 cut(s) 454, 508, 604, 673, 781
BspFNI CGCG 2 cut(s) 174, 219
BspLI GGNNCC 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 739
BspTNI GGTCTC 1 cut(s) 664
BsrBI CCGCTC 2 cut(s) 271, 285
BsrI ACTGG 3 cut(s) 242, 425, 764
BssECI CCNNGG 4 cut(s) 60, 124, 217, 638
BssMI GATC 4 cut(s) 448, 491, 661, 835
BssNI GRCGYC 2 cut(s) 20, 318
BssT1I CCWWGG 1 cut(s) 638
Bst4CI ACNGT 5 cut(s) 253, 550, 559, 623, 779
Bst6I CTCTTC 1 cut(s) 290
BstACI GRCGYC 2 cut(s) 20, 318
BstC8I GCNNGC 2 cut(s) 202, 276
BstDEI CTNAG 5 cut(s) 441, 495, 612, 681, 789
BstDSI CCRYGG 1 cut(s) 217
BstENI CCTNNNNNAGG 1 cut(s) 809
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 2 cut(s) 174, 219
BstKTI GATC 4 cut(s) 451, 494, 664, 838
BstMAI GTCTC 4 cut(s) 664, 671, 695, 810
BstMBI GATC 4 cut(s) 448, 491, 661, 835
BstMCI CGRYCG 3 cut(s) 172, 217, 226
BstMWI GCNNNNNNNGC 4 cut(s) 135, 222, 333, 336
BstSCI CCNGG 2 cut(s) 37, 124
BstUI CGCG 2 cut(s) 174, 219
BstV1I GCAGC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
BstZI CGGCCG 2 cut(s) 214, 223
BsuRI GGCC 4 cut(s) 216, 225, 360, 461
BtgI CCRYGG 1 cut(s) 217
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 838
BtsIMutI CAGTG 2 cut(s) 235, 645
Cac8I GCNNGC 2 cut(s) 202, 276
Cfr42I CCGCGG 1 cut(s) 220
Csp6I GTAC 3 cut(s) 166, 436, 560
CviAII CATG 2 cut(s) 534, 581
CviJI RGCY 9 cut(s) 70, 138, 157, 216, 225, 360, 372, 461, 709
CviKI_1 RGCY 9 cut(s) 70, 138, 157, 216, 225, 360, 372, 461, 709
CviQI GTAC 3 cut(s) 166, 436, 560
DdeI CTNAG 5 cut(s) 441, 495, 612, 681, 789
DpnI GATC 4 cut(s) 450, 493, 663, 837
DpnII GATC 4 cut(s) 448, 491, 661, 835
DriI GACNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 3 cut(s) 214, 223, 358
EagI CGGCCG 2 cut(s) 214, 223
Eam1104I CTCTTC 1 cut(s) 290
Eam1105I GACNNNNNGTC 1 cut(s) 781
EarI CTCTTC 1 cut(s) 290
EciI GGCGGA 1 cut(s) 354
Ecl136II GAGCTC 1 cut(s) 157
EclXI CGGCCG 2 cut(s) 214, 223
Eco130I CCWWGG 1 cut(s) 638
Eco147I AGGCCT 1 cut(s) 461
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 664
Eco52I CGGCCG 2 cut(s) 214, 223
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 2 cut(s) 440, 561
EcoICRI GAGCTC 1 cut(s) 157
EcoNI CCTNNNNNAGG 1 cut(s) 809
EcoRI GAATTC 2 cut(s) 391, 573
EcoT14I CCWWGG 1 cut(s) 638
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 638
FaeI CATG 2 cut(s) 537, 584
FaiI YATR 8 cut(s) 198, 512, 535, 582, 594, 603, 809, 824
FaqI GGGAC 1 cut(s) 329
FatI CATG 2 cut(s) 533, 580
FauI CCCGC 3 cut(s) 98, 267, 278
FbaI TGATCA 1 cut(s) 448
FblI GTMKAC 1 cut(s) 687
FokI GGATG 1 cut(s) 845
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 1 cut(s) 639
HaeIII GGCC 4 cut(s) 216, 225, 360, 461
HapII CCGG 6 cut(s) 39, 126, 191, 226, 361, 674
Hin1I GRCGYC 2 cut(s) 20, 318
Hin1II CATG 2 cut(s) 537, 584
HinfI GANTC 3 cut(s) 48, 116, 703
HpaII CCGG 6 cut(s) 39, 126, 191, 226, 361, 674
HphI GGTGA 1 cut(s) 635
Hpy166II GTNNAC 1 cut(s) 688
Hpy188I TCNGA 6 cut(s) 61, 112, 121, 517, 726, 790
Hpy188III TCNNGA 1 cut(s) 611
Hpy8I GTNNAC 1 cut(s) 688
Hpy99I CGWCG 5 cut(s) 22, 25, 116, 323, 326
HpyAV CCTTC 4 cut(s) 259, 295, 300, 340
HpyCH4III ACNGT 5 cut(s) 253, 550, 559, 623, 779
HpyCH4IV ACGT 3 cut(s) 20, 318, 562
HpyF10VI GCNNNNNNNGC 4 cut(s) 135, 222, 333, 336
HpyF3I CTNAG 5 cut(s) 441, 495, 612, 681, 789
HpySE526I ACGT 3 cut(s) 20, 318, 562
Hsp92I GRCGYC 2 cut(s) 20, 318
Hsp92II CATG 2 cut(s) 537, 584
Ksp22I TGATCA 1 cut(s) 448
KspI CCGCGG 1 cut(s) 220
Kzo9I GATC 4 cut(s) 448, 491, 661, 835
LmnI GCTCC 4 cut(s) 64, 75, 268, 282
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 1 cut(s) 802
MaeI CTAG 1 cut(s) 639
MaeII ACGT 3 cut(s) 20, 318, 562
MaeIII GTNAC 2 cut(s) 563, 623
MalI GATC 4 cut(s) 450, 493, 663, 837
MbiI CCGCTC 2 cut(s) 271, 285
MboI GATC 4 cut(s) 448, 491, 661, 835
MboII GAAGA 3 cut(s) 307, 446, 554
MflI RGATCY 1 cut(s) 491
MhlI GDGCHC 2 cut(s) 159, 429
MluCI AATT 2 cut(s) 391, 573
MlyI GAGTC 2 cut(s) 125, 697
MmeI TCCRAC 3 cut(s) 135, 144, 810
MseI TTAA 3 cut(s) 150, 527, 749
MspA1I CMGCKG 1 cut(s) 219
MspI CCGG 6 cut(s) 39, 126, 191, 226, 361, 674
MspR9I CCNGG 2 cut(s) 39, 126
MvnI CGCG 2 cut(s) 174, 219
MwoI GCNNNNNNNGC 4 cut(s) 135, 222, 333, 336
NciI CCSGG 2 cut(s) 39, 126
NdeII GATC 4 cut(s) 448, 491, 661, 835
NlaIII CATG 2 cut(s) 537, 584
NlaIV GGNNCC 1 cut(s) 741
NmeAIII GCCGAG 2 cut(s) 503, 685
NmuCI GTSAC 2 cut(s) 563, 623
PceI AGGCCT 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfeI GAWTC 1 cut(s) 48
PflFI GACNNNGTC 1 cut(s) 320
PleI GAGTC 2 cut(s) 124, 697
PpsI GAGTC 2 cut(s) 124, 697
Psp124BI GAGCTC 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 741
PsuI RGATCY 1 cut(s) 491
PsyI GACNNNGTC 1 cut(s) 320
RsaI GTAC 3 cut(s) 167, 437, 561
RsaNI GTAC 3 cut(s) 166, 436, 560
SacI GAGCTC 1 cut(s) 159
SacII CCGCGG 1 cut(s) 220
SaqAI TTAA 3 cut(s) 150, 527, 749
Sau3AI GATC 4 cut(s) 448, 491, 661, 835
SchI GAGTC 2 cut(s) 125, 697
ScrFI CCNGG 2 cut(s) 39, 126
SduI GDGCHC 2 cut(s) 159, 429
SfaNI GCATC 1 cut(s) 802
SfiI GGCCNNNNNGGCC 1 cut(s) 222
Sfr303I CCGCGG 1 cut(s) 220
SgrBI CCGCGG 1 cut(s) 220
Sse9I AATT 2 cut(s) 391, 573
SseBI AGGCCT 1 cut(s) 461
SspMI CTAG 1 cut(s) 639
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 37, 124
StyI CCWWGG 1 cut(s) 638
TaaI ACNGT 5 cut(s) 253, 550, 559, 623, 779
TaiI ACGT 3 cut(s) 23, 321, 565
TaqI TCGA 2 cut(s) 159, 843
TasI AATT 2 cut(s) 391, 573
TfiI GAWTC 1 cut(s) 48
Tru1I TTAA 3 cut(s) 150, 527, 749
Tru9I TTAA 3 cut(s) 150, 527, 749
TscAI CASTG 2 cut(s) 242, 652
TseFI GTSAC 2 cut(s) 563, 623
TseI GCWGC 1 cut(s) 67
Tsp45I GTSAC 2 cut(s) 563, 623
TspDTI ATGAA 2 cut(s) 522, 811
TspRI CASTG 2 cut(s) 242, 652
Tth111I GACNNNGTC 1 cut(s) 320
XagI CCTNNNNNAGG 1 cut(s) 809
XapI RAATTY 2 cut(s) 391, 573
XmaJI CCTAGG 1 cut(s) 638
XmiI GTMKAC 1 cut(s) 687
XspI CTAG 1 cut(s) 639
ZraI GACGTC 2 cut(s) 21, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.